AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044253

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTAGTCTTTGCAAATTT+TGG 0.090976 8:+57807927 None:intergenic
AAATTGCTTCTTGTGTTAAA+TGG 0.133780 8:-57808062 MsaT044253.1:CDS
TCCTTACCATCCTTCTTATT+TGG 0.212526 8:+57809719 None:intergenic
ATAGCTGTGTGGAGAAAATT+TGG 0.222355 8:-57808098 MsaT044253.1:CDS
TTCTACTTCGTCAGGAATAT+TGG 0.232123 8:+57807959 None:intergenic
TTTAATTCATCATCACAATT+TGG 0.253101 8:+57809905 None:intergenic
AGGACAGATGATTTGATTCT+TGG 0.289591 8:-57807984 MsaT044253.1:CDS
AATTGCTTCTTGTGTTAAAT+GGG 0.315429 8:-57808061 MsaT044253.1:CDS
TCAAATACTTGTCCAATTGT+AGG 0.344020 8:+57809881 None:intergenic
AAACAATTGGATGGATTTGA+AGG 0.422078 8:-57808023 MsaT044253.1:CDS
GGAAGGATTTCTACTTCGTC+AGG 0.426183 8:+57807951 None:intergenic
GATGAATTAAAGCCTACAAT+TGG 0.432711 8:-57809893 MsaT044253.1:CDS
GGACAGATGATTTGATTCTT+GGG 0.434817 8:-57807983 MsaT044253.1:CDS
CCAAAATTTGCAAAGACTAA+AGG 0.474060 8:-57807927 MsaT044253.1:CDS
GAGAAAATTTGGAAAAGATA+AGG 0.485689 8:-57808087 MsaT044253.1:CDS
TCTCCACACAGCTATGTAAG+TGG 0.490166 8:+57808106 None:intergenic
GGGTTATTTGCCAAATAAGA+AGG 0.490320 8:-57809729 MsaT044253.1:CDS
GTTAAATGGGGTTGTTAACA+TGG 0.491983 8:-57808048 MsaT044253.1:CDS
GCCAAATAAGAAGGATGGTA+AGG 0.493986 8:-57809720 MsaT044253.1:CDS
TGACATAGCTGACAATGTCT+TGG 0.510088 8:-57808163 MsaT044253.1:intron
TGGAAGAATGGCATAGAAGA+AGG 0.510515 8:-57807656 MsaT044253.1:CDS
ATGGCATAGAAGAAGGATGA+AGG 0.534902 8:-57807649 MsaT044253.1:CDS
GGTAGTGGTAAAAGAATCAA+AGG 0.539060 8:-57807906 MsaT044253.1:CDS
AAGAATCAAATCATCTGTCC+TGG 0.550254 8:+57807986 None:intergenic
TTTGCAAAGACTAAAGGTAG+TGG 0.573810 8:-57807921 MsaT044253.1:CDS
AACAATTGGATGGATTTGAA+GGG 0.574388 8:-57808022 MsaT044253.1:CDS
ACAAACTCATTTCTGATGTG+TGG 0.578358 8:-57808131 MsaT044253.1:CDS
TACATTTGAAAGTTTCACAT+AGG 0.579229 8:+57812325 None:intergenic
AATCAAAGGTGGAAAAGAGA+AGG 0.592127 8:-57807892 MsaT044253.1:CDS
TATTTGCCAAATAAGAAGGA+TGG 0.592394 8:-57809725 MsaT044253.1:CDS
AAGGGAGTTCTAAGAGAACC+AGG 0.594888 8:-57808004 MsaT044253.1:CDS
GCAAAGTGAATCTACTGTCA+AGG 0.595204 8:-57809696 MsaT044253.1:intron
GTATTTGATACGTTAGCAGA+AGG 0.613843 8:-57809866 MsaT044253.1:CDS
AGTGGTAAAAGAATCAAAGG+TGG 0.614504 8:-57807903 MsaT044253.1:CDS
GCAATACTTTGTAAGTCATG+CGG 0.649820 8:-57807846 MsaT044253.1:intron
ATTGCTTCTTGTGTTAAATG+GGG 0.659997 8:-57808060 MsaT044253.1:CDS
GAACCACTTACATAGCTGTG+TGG 0.775450 8:-57808109 MsaT044253.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 57807629 57812357 57807629 ID=MsaG044253
Chr8 mRNA 57807629 57812357 57807629 ID=MsaT044253.1;Parent=MsaG044253
Chr8 exon 57807629 57807681 57807629 ID=MsaT044253.1.exon4;Parent=MsaT044253.1
Chr8 CDS 57807629 57807681 57807629 ID=cds.MsaT044253.1;Parent=MsaT044253.1
Chr8 exon 57807847 57808177 57807847 ID=MsaT044253.1.exon3;Parent=MsaT044253.1
Chr8 CDS 57807847 57808177 57807847 ID=cds.MsaT044253.1;Parent=MsaT044253.1
Chr8 exon 57809697 57809942 57809697 ID=MsaT044253.1.exon2;Parent=MsaT044253.1
Chr8 CDS 57809697 57809942 57809697 ID=cds.MsaT044253.1;Parent=MsaT044253.1
Chr8 exon 57812328 57812357 57812328 ID=MsaT044253.1.exon1;Parent=MsaT044253.1
Chr8 CDS 57812328 57812357 57812328 ID=cds.MsaT044253.1;Parent=MsaT044253.1
Gene Sequence

>MsaT044253.1

ATGAGTAGTGCCTATGTGAAACTTTCAAATGAATATGAATGTGTGCCAAATTGTGATGATGAATTAAAGCCTACAATTGGACAAGTATTTGATACGTTAGCAGAAGGTAAAAATTTTTACGAAAAATATGCACTCTCTGTCGGATTTAGCGTGCGTTCATCATCATCGACTATAGATAAAAACGGCGTGAAGCGTTGGAAGTATTTTGTTTGCTCGAAAGAGGGTTATTTGCCAAATAAGAAGGATGGTAAGGAGCAAAGTGAATCTACTGTCAAGCTGACAATGTCTTGGAAGAAGACAACAAACTCATTTCTGATGTGTGGAACCACTTACATAGCTGTGTGGAGAAAATTTGGAAAAGATAAGGAAAAATTGCTTCTTGTGTTAAATGGGGTTGTTAACATGGAAAAACAATTGGATGGATTTGAAGGGAGTTCTAAGAGAACCAGGACAGATGATTTGATTCTTGGGTCCAATATTCCTGACGAAGTAGAAATCCTTCCACCAAAATTTGCAAAGACTAAAGGTAGTGGTAAAAGAATCAAAGGTGGAAAAGAGAAGGCTATTGAACAACAACAAAAAAAAGCAATACTTTGTAAGTCATGCGGATTGGAAGAATGGCATAGAAGAAGGATGAAGGAAGTTAGACAAACAAGATGA
Protein sequence

>MsaT044253.1

MSSAYVKLSNEYECVPNCDDELKPTIGQVFDTLAEGKNFYEKYALSVGFSVRSSSSTIDKNGVKRWKYFVCSKEGYLPNKKDGKEQSESTVKLTMSWKKTTNSFLMCGTTYIAVWRKFGKDKEKLLLVLNGVVNMEKQLDGFEGSSKRTRTDDLILGSNIPDEVEILPPKFAKTKGSGKRIKGGKEKAIEQQQKKAILCKSCGLEEWHRRRMKEVRQTR*