AlfalfaGEDB | MsaG044269

M. sativa ssp. caerulea / MsaG044269

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009937 GO:0009938 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010476 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0080090 GO:0090567 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF05142.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044269	XP_013446370.1	95.575	339	7	3	1	339	1	331	0.0	658

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044269	sp\|Q9SD40\|SRS1_ARATH	44.784	393	140	12	1	339	1	370	7.10e-79	248

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044269	tr\|A0A072TSM8\|A0A072TSM8_MEDTR	95.575	339	7	3	1	339	1	331	0.0	658

TFs/TRs:

Gene ID	Type	Classification
MsaG044269	TF	SRS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG010790	MsaG044269	0.801316	1.597690e-48	2.225347e-46
MsaG043410	MsaG044269	0.812258	7.974059e-51	1.439126e-48
MsaG044269	MsaG044609	0.803791	4.962869e-49	7.315934e-47
MsaG044269	MsaG031596	0.805642	2.046936e-49	3.150003e-47
MsaG018989	MsaG044269	0.803876	4.765387e-49	7.038647e-47
MsaG020137	MsaG044269	0.823517	2.345644e-53	5.667003e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044269	MtrunA17_Chr8g0372461	95.575	339	7	3	1	339	1	331	0.0	658
MsaG044269	MtrunA17_Chr5g0404781	52.198	364	108	13	1	339	1	323	4.54e-91	275
MsaG044269	MtrunA17_Chr3g0142171	42.939	347	126	16	1	334	1	288	3.91e-63	202
MsaG044269	MtrunA17_Chr1g0155791	44.205	371	142	15	1	339	1	338	2.72e-58	191
MsaG044269	MtrunA17_Chr5g0441921	50.323	155	54	4	129	260	124	278	8.11e-41	145
MsaG044269	MtrunA17_Chr4g0035591	47.887	142	65	2	125	266	16	148	3.13e-40	140
MsaG044269	MtrunA17_Chr3g0082511	39.869	153	69	1	129	258	134	286	1.50e-33	126
MsaG044269	MtrunA17_Chr8g0353111	39.884	173	73	3	116	258	111	282	4.17e-33	125
MsaG044269	MtrunA17_Chr0c01g0489181	56.410	39	17	0	125	163	63	101	5.82e-14	67.4
MsaG044269	MtrunA17_Chr2g0281741	61.538	39	15	0	125	163	146	184	4.09e-13	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044269	AT3G51060.1	44.784	393	140	12	1	339	1	370	7.22e-80	248
MsaG044269	AT5G66350.1	46.257	374	123	16	1	339	1	331	1.77e-73	231
MsaG044269	AT5G66350.3	46.006	363	120	15	1	328	1	322	4.56e-71	224
MsaG044269	AT5G66350.2	46.133	362	119	15	1	327	1	321	6.21e-71	224
MsaG044269	AT1G19790.2	42.667	375	147	16	1	338	1	344	6.94e-69	219
MsaG044269	AT1G19790.1	42.667	375	147	16	1	338	1	344	6.94e-69	219
MsaG044269	AT1G75520.1	41.711	374	155	14	1	339	1	346	1.39e-63	206
MsaG044269	AT4G36260.1	39.779	362	150	13	1	337	1	319	3.69e-54	180
MsaG044269	AT2G21400.2	47.701	174	83	3	126	295	7	176	6.39e-49	162
MsaG044269	AT2G21400.4	47.701	174	83	3	126	295	6	175	8.02e-49	162
MsaG044269	AT2G21400.3	47.647	170	74	3	126	295	7	161	3.07e-48	160
MsaG044269	AT2G21400.1	47.647	170	74	3	126	295	6	160	3.62e-48	160
MsaG044269	AT2G18120.1	53.548	155	57	3	118	263	61	209	3.26e-47	159
MsaG044269	AT2G18120.2	52.288	153	60	2	118	263	61	207	1.62e-46	157
MsaG044269	AT5G12330.1	47.205	161	55	4	129	260	112	271	2.33e-38	139
MsaG044269	AT5G12330.2	47.205	161	55	4	129	260	112	271	2.33e-38	139
MsaG044269	AT5G12330.4	47.205	161	55	4	129	260	199	358	4.38e-38	140
MsaG044269	AT5G33210.1	42.405	158	70	5	95	237	14	165	4.82e-29	110
MsaG044269	AT5G33210.2	61.176	85	23	2	127	201	15	99	4.01e-27	103
MsaG044269	AT3G54430.1	42.553	141	59	6	129	258	41	170	9.65e-26	102
MsaG044269	AT5G12330.3	82.609	46	8	0	129	174	112	157	3.78e-21	90.9

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATTGTTGGTACTACTTTC+TGG	0.121760	8:-58115998	None:intergenic
AAAGGAATTCTCTATGATTT+TGG	0.200894	8:+58115974	MsaT044269.1:CDS
ACAGAGGTAATTTAGAATTA+TGG	0.238498	8:+58114663	MsaT044269.1:CDS
CACCACAAGAGACACTATTT+TGG	0.271662	8:+58114609	MsaT044269.1:CDS
TGATCCTTCTTCATTGTATT+CGG	0.283339	8:+58116159	MsaT044269.1:CDS
AATAATAGTAATTACATGAT+TGG	0.330195	8:+58116040	MsaT044269.1:CDS
ATAATAGTAATTACATGATT+GGG	0.347728	8:+58116041	MsaT044269.1:CDS
TACCACTGTTGTTGCCTCCT+CGG	0.375579	8:+58116126	MsaT044269.1:CDS
ATGGCTGGTTTGTTCTCATT+AGG	0.380675	8:+58114530	MsaT044269.1:CDS
TAAGCTCACCAGCGGAGTTC+AGG	0.381652	8:+58115870	MsaT044269.1:CDS
CAGCGGAGTTCAGGTGTGTA+AGG	0.390315	8:+58115879	MsaT044269.1:CDS
GTTGTTGTGATTCTTCTCCT+TGG	0.392328	8:-58114574	None:intergenic
TACATGATTGGGGAGACTTC+CGG	0.393571	8:+58116052	MsaT044269.1:CDS
GTTGTTGCTGGTGGTGTTGT+TGG	0.401531	8:-58114709	None:intergenic
GTTGTTGGTGCTGTTGTTGT+TGG	0.405595	8:-58114694	None:intergenic
ATAACTGTTGTTGACGTTCA+CGG	0.415997	8:-58115039	None:intergenic
TACGGTCCAGGAGTTGGTTT+AGG	0.417465	8:+58114782	MsaT044269.1:CDS
ACCACTGTTGTTGCCTCCTC+GGG	0.418082	8:+58116127	MsaT044269.1:CDS
ACTATTGTTGCTGTTATTGT+TGG	0.418687	8:-58116011	None:intergenic
CCAGGAGTTGGTTTAGGTGT+TGG	0.426741	8:+58114788	MsaT044269.1:CDS
GTTTAGGTGTTGGACCAAGC+AGG	0.429835	8:+58114798	MsaT044269.1:CDS
GGTGCTGTTGTTGTTGGTGT+TGG	0.440333	8:-58114688	None:intergenic
TGGTATCAGAGTCGGTGATT+AGG	0.442544	8:-58116108	None:intergenic
GCCGTCATGAAGGCATTAAT+CGG	0.448399	8:-58116184	None:intergenic
TTGTTCTCATTAGGAGGTGG+TGG	0.459228	8:+58114539	MsaT044269.1:CDS
TTCTCATTAGGAGGTGGTGG+TGG	0.461573	8:+58114542	MsaT044269.1:CDS
GAGGACAATCTTTCTTAGCT+TGG	0.464592	8:-58114931	None:intergenic
TTCTTCTTCAAAGAAATGGC+TGG	0.464677	8:+58114515	None:intergenic
AACGCCAACACCGCCTTCAC+CGG	0.468847	8:-58116071	None:intergenic
TTTGAAATATCTCTTTGTAA+AGG	0.469835	8:-58115070	None:intergenic
GAGATCGGCAGCGTTTACTA+TGG	0.472056	8:+58114856	MsaT044269.1:CDS
ACTTCCGGTGAAGGCGGTGT+TGG	0.482952	8:+58116067	MsaT044269.1:CDS
CAATTGATGATGCAGATGAT+AGG	0.486383	8:+58115909	MsaT044269.1:CDS
TTAGGAGGTGGTGGTGGAAG+AGG	0.490541	8:+58114548	MsaT044269.1:CDS
AAACTCATGTTAAAAGCACT+TGG	0.502171	8:+58114999	MsaT044269.1:CDS
ACATCAATCGTCGTCGAGAT+CGG	0.503948	8:+58114841	MsaT044269.1:CDS
GGTTTGTTCTCATTAGGAGG+TGG	0.504419	8:+58114536	MsaT044269.1:CDS
GCTGGTGAGCTTACCACAGC+AGG	0.505962	8:-58115860	None:intergenic
CGTTCACGGCGTCTAGAAGC+AGG	0.507852	8:-58115025	None:intergenic
GATCTCTACGGTCCAGGAGT+TGG	0.516227	8:+58114776	MsaT044269.1:CDS
GCTGGTTTGTTCTCATTAGG+AGG	0.517479	8:+58114533	MsaT044269.1:CDS
ATTGGAGGACATTTGTTCAA+AGG	0.517913	8:+58115956	MsaT044269.1:CDS
CCGCGAGATCTCTACGGTCC+AGG	0.520475	8:+58114770	MsaT044269.1:CDS
AGAGCATTTGAACCATCTCT+AGG	0.524317	8:-58115097	None:intergenic
ATTGGGGAGACTTCCGGTGA+AGG	0.525108	8:+58116058	MsaT044269.1:CDS
AGGTCTTCTTCTTCAGCTGA+AGG	0.529357	8:+58114884	MsaT044269.1:CDS
TTTAGGTGTTGGACCAAGCA+GGG	0.533902	8:+58114799	MsaT044269.1:CDS
GGGATAAGTTGTCAAGATTG+TGG	0.548229	8:+58114905	MsaT044269.1:CDS
CCAACACCTAAACCAACTCC+TGG	0.554565	8:-58114788	None:intergenic
CAAAATAGTGTCTCTTGTGG+TGG	0.555466	8:-58114608	None:intergenic
TAGAGATCTCGCGGGAAGAA+CGG	0.556385	8:-58114761	None:intergenic
TATCAAACGGCTGTTAACAT+TGG	0.556930	8:+58115938	MsaT044269.1:CDS
TGATAGGTATGCATATCAAA+CGG	0.559733	8:+58115925	MsaT044269.1:CDS
CAAACGGCTGTTAACATTGG+AGG	0.566332	8:+58115941	MsaT044269.1:CDS
CGTACCACTAGCCGTCATGA+AGG	0.569674	8:-58116194	None:intergenic
ATTTCAAAACGCCCTAGAGA+TGG	0.570524	8:+58115085	MsaT044269.1:CDS
TTCTTCCCGCGAGATCTCTA+CGG	0.572634	8:+58114764	MsaT044269.1:CDS
TCATCTGATGAAACCCTGCT+TGG	0.577114	8:-58114812	None:intergenic
AACTCATGTTAAAAGCACTT+GGG	0.578823	8:+58115000	MsaT044269.1:CDS
CTTACACACCTGAACTCCGC+TGG	0.583863	8:-58115878	None:intergenic
TAATAGTAATTACATGATTG+GGG	0.584644	8:+58116042	MsaT044269.1:CDS
GGTCTTCTTCTTCAGCTGAA+GGG	0.597588	8:+58114885	MsaT044269.1:CDS
GAGCATTTGAACCATCTCTA+GGG	0.600057	8:-58115096	None:intergenic
AGCGGAGTTCAGGTGTGTAA+GGG	0.600996	8:+58115880	MsaT044269.1:CDS
AGGATCAACAAGTGCGCCCG+AGG	0.604938	8:-58116143	None:intergenic
GGAGCCGAATACAATGAAGA+AGG	0.606433	8:-58116163	None:intergenic
GAGTCGGTGATTAGGTTCAA+CGG	0.611827	8:-58116100	None:intergenic
AATGCCTTCATGACGGCTAG+TGG	0.616126	8:+58116190	MsaT044269.1:CDS
ATCAACAAGTGCGCCCGAGG+AGG	0.617141	8:-58116140	None:intergenic
GGGGAGACTTCCGGTGAAGG+CGG	0.620239	8:+58116061	MsaT044269.1:CDS
AACAACAGTGGTATCAGAGT+CGG	0.622301	8:-58116116	None:intergenic
TCCGATTAATGCCTTCATGA+CGG	0.631102	8:+58116183	MsaT044269.1:CDS
TGCTGTGGTAAGCTCACCAG+CGG	0.638941	8:+58115862	MsaT044269.1:CDS
GATGTTTCATCTTACAACAG+AGG	0.641152	8:+58114647	MsaT044269.1:CDS
CCTGGACCGTAGAGATCTCG+CGG	0.642572	8:-58114770	None:intergenic
AGAACTTGTTGTAAGAGTCG+CGG	0.648244	8:+58114965	MsaT044269.1:CDS
CAGCGTTTACTATGGCTATG+AGG	0.650670	8:+58114864	MsaT044269.1:CDS
TACCAAAATAGTGTCTCTTG+TGG	0.654055	8:-58114611	None:intergenic
AAGATGATATGCATGCTGCA+CGG	0.670001	8:+58114738	MsaT044269.1:CDS
GCCCGAGGAGGCAACAACAG+TGG	0.675386	8:-58116128	None:intergenic
GGTGGTGGAAGAGGAAACCA+AGG	0.679788	8:+58114557	MsaT044269.1:CDS
CAAGTTCTACATCTCATGTG+AGG	0.701177	8:-58114950	None:intergenic
CTGGACCGTAGAGATCTCGC+GGG	0.715374	8:-58114769	None:intergenic
GGAGAAGAATCACAACAACA+AGG	0.732085	8:+58114578	MsaT044269.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	58114530	58116243	58114530	ID=MsaG044269
Chr8	mRNA	58114530	58116243	58114530	ID=MsaT044269.1;Parent=MsaG044269
Chr8	exon	58114530	58115145	58114530	ID=MsaT044269.1.exon1;Parent=MsaT044269.1
Chr8	CDS	58114530	58115145	58114530	ID=cds.MsaT044269.1;Parent=MsaT044269.1
Chr8	exon	58115840	58116243	58115840	ID=MsaT044269.1.exon2;Parent=MsaT044269.1
Chr8	CDS	58115840	58116243	58115840	ID=cds.MsaT044269.1;Parent=MsaT044269.1

Gene Sequence

>MsaT044269.1

ATGGCTGGTTTGTTCTCATTAGGAGGTGGTGGTGGAAGAGGAAACCAAGGAGAAGAATCACAACAACAAGGTCATATTCCACCACAAGAGACACTATTTTGGTACAACAAAAACGATGATGTTTCATCTTACAACAGAGGTAATTTAGAATTATGGAACCAACACCAACAACAACAGCACCAACAACACCACCAGCAACAACAACAACAAGATGATATGCATGCTGCACGGCCGTTCTTCCCGCGAGATCTCTACGGTCCAGGAGTTGGTTTAGGTGTTGGACCAAGCAGGGTTTCATCAGATGATCATGAACATCAATCGTCGTCGAGATCGGCAGCGTTTACTATGGCTATGAGGTCTTCTTCTTCAGCTGAAGGGATAAGTTGTCAAGATTGTGGTAACCAAGCTAAGAAAGATTGTCCTCACATGAGATGTAGAACTTGTTGTAAGAGTCGCGGCTTTCAGTGTCAAACTCATGTTAAAAGCACTTGGGTTCCTGCTTCTAGACGCCGTGAACGTCAACAACAGTTATCTTCTTCTCCTTTACAAAGAGATATTTCAAAACGCCCTAGAGATGGTTCAAATGCTCTTGTTTCAACTCGTAATTTTCCTTCAGGGTTAGAGGAAGCAAATTTTCCTGCTGTGGTAAGCTCACCAGCGGAGTTCAGGTGTGTAAGGGTTAGTTCAATTGATGATGCAGATGATAGGTATGCATATCAAACGGCTGTTAACATTGGAGGACATTTGTTCAAAGGAATTCTCTATGATTTTGGTCCAGAAAGTAGTACCAACAATAACAGCAACAATAGTAATTATAATAATAGTAATTACATGATTGGGGAGACTTCCGGTGAAGGCGGTGTTGGCGTTGCTCAACCGTTGAACCTAATCACCGACTCTGATACCACTGTTGTTGCCTCCTCGGGCGCACTTGTTGATCCTTCTTCATTGTATTCGGCTCCGATTAATGCCTTCATGACGGCTAGTGGTACGCAATTTTTCCCTCGCCCAAGATCTTGA

Protein sequence

>MsaT044269.1

MAGLFSLGGGGGRGNQGEESQQQGHIPPQETLFWYNKNDDVSSYNRGNLELWNQHQQQQHQQHHQQQQQQDDMHAARPFFPRDLYGPGVGLGVGPSRVSSDDHEHQSSSRSAAFTMAMRSSSSAEGISCQDCGNQAKKDCPHMRCRTCCKSRGFQCQTHVKSTWVPASRRRERQQQLSSSPLQRDISKRPRDGSNALVSTRNFPSGLEEANFPAVVSSPAEFRCVRVSSIDDADDRYAYQTAVNIGGHLFKGILYDFGPESSTNNNSNNSNYNNSNYMIGETSGEGGVGVAQPLNLITDSDTTVVASSGALVDPSSLYSAPINAFMTASGTQFFPRPRS*