AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044320 MtrunA17_Chr8g0373111 97.222 72 2 0 1 72 1 72 2.71e-49 149
MsaG044320 MtrunA17_Chr8g0373121 97.222 72 2 0 1 72 1 72 2.71e-49 149
MsaG044320 MtrunA17_Chr8g0373131 95.833 72 3 0 1 72 1 72 8.54e-49 148
MsaG044320 MtrunA17_Chr5g0404301 80.645 62 12 0 3 64 2 63 2.39e-35 114
MsaG044320 MtrunA17_Chr8g0373101 70.000 70 20 1 1 70 1 69 5.07e-33 108
MsaG044320 MtrunA17_Chr3g0144631 64.615 65 22 1 5 68 16 80 1.34e-27 95.1
MsaG044320 MtrunA17_Chr1g0155181 55.224 67 29 1 2 67 4 70 7.77e-26 90.5
MsaG044320 MtrunA17_Chr6g0449111 56.061 66 28 1 2 66 7 72 6.92e-25 88.2
MsaG044320 MtrunA17_Chr5g0404101 54.688 64 28 1 5 67 19 82 9.82e-25 88.2
MsaG044320 MtrunA17_Chr3g0141601 54.286 70 31 1 2 70 4 73 8.22e-24 85.5
MsaG044320 MtrunA17_Chr3g0081631 55.072 69 29 1 2 68 7 75 9.87e-23 82.8
MsaG044320 MtrunA17_Chr7g0255841 58.065 62 25 1 7 67 14 75 3.77e-22 81.6
MsaG044320 MtrunA17_Chr5g0429521 56.338 71 30 1 1 70 25 95 1.62e-21 84.7
MsaG044320 MtrunA17_Chr3g0104071 55.882 68 29 1 2 68 40 107 1.66e-20 82.0
MsaG044320 MtrunA17_Chr3g0141641 55.385 65 28 1 4 67 2 66 9.06e-20 75.5
MsaG044320 MtrunA17_Chr7g0241761 52.857 70 32 1 2 70 32 101 1.29e-18 77.0
MsaG044320 MtrunA17_Chr8g0365011 43.836 73 40 1 1 72 4 76 4.78e-17 72.4
MsaG044320 MtrunA17_Chr5g0436501 52.308 65 30 1 3 66 25 89 8.46e-17 71.6
MsaG044320 MtrunA17_Chr1g0194071 48.571 70 34 1 3 70 9 78 6.02e-16 65.9
MsaG044320 MtrunA17_Chr7g0270711 52.459 61 27 1 3 61 9 69 2.14e-15 64.3
MsaG044320 MtrunA17_Chr1g0162081 38.571 70 42 1 2 70 9 78 7.14e-14 63.9
MsaG044320 MtrunA17_Chr8g0364741 37.500 72 44 1 2 72 7 78 2.02e-13 60.1
MsaG044320 MtrunA17_Chr1g0210471 40.000 65 38 1 3 66 22 86 1.07e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044320 AT4G39250.1 64.615 65 22 1 5 68 14 78 4.17e-28 97.1
MsaG044320 AT1G75250.2 62.687 67 24 1 3 68 10 76 5.43e-27 94.4
MsaG044320 AT1G75250.1 64.615 65 22 1 5 68 12 76 7.84e-27 94.7
MsaG044320 AT2G21650.1 56.522 69 29 1 5 72 14 82 9.49e-26 91.3
MsaG044320 AT1G19510.1 61.290 62 23 1 8 68 15 76 1.11e-23 85.9
MsaG044320 AT2G38090.1 52.857 70 32 1 2 70 26 95 6.12e-19 78.2
MsaG044320 AT2G18328.1 55.385 65 27 2 8 71 14 77 1.47e-18 72.4
MsaG044320 AT5G58900.1 50.725 69 33 1 2 69 31 99 3.01e-17 73.6
MsaG044320 AT5G05790.2 47.143 70 36 1 2 70 28 97 1.76e-16 71.2
MsaG044320 AT5G05790.1 47.143 70 36 1 2 70 28 97 1.76e-16 71.2
MsaG044320 AT3G11280.2 50.794 63 30 1 5 66 33 95 4.82e-16 70.1
MsaG044320 AT3G11280.1 50.794 63 30 1 5 66 33 95 4.82e-16 70.1
MsaG044320 AT4G36570.1 65.909 44 14 1 5 47 12 55 7.71e-16 65.1
MsaG044320 AT5G08520.1 47.826 69 35 1 5 72 12 80 4.76e-14 64.7
MsaG044320 AT5G04760.1 43.284 67 37 1 1 66 1 67 1.74e-13 62.4
MsaG044320 AT1G49010.1 42.857 70 36 2 1 66 4 73 1.01e-12 61.2

Find 13 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACATTGTGCCATCTTTC+AGG 0.193556 8:-58626429 None:intergenic
ATGGCACAATGTTGCCAAAA+TGG 0.377575 8:+58626437 MsaT044320.1:CDS
TGTAACGAGGAAAAGGGATA+TGG 0.417505 8:-58626533 None:intergenic
TACGTTGAACATCTTCTTTG+AGG 0.435409 8:-58626500 None:intergenic
ATCAACAAATGGCAACCTCT+TGG 0.450563 8:+58626349 None:intergenic
CAAACTGTTTGAACAAGCAT+TGG 0.467435 8:+58626386 MsaT044320.1:CDS
CACAATGTTGCCAAAATGGT+TGG 0.522014 8:+58626441 MsaT044320.1:CDS
TGGCAACCTCTTGGACTGCA+AGG 0.539984 8:+58626358 MsaT044320.1:CDS
ATAGAGAAACTCCTGAAAGA+TGG 0.546752 8:+58626418 MsaT044320.1:CDS
AAAACTAACACCAACTCCTA+AGG 0.547909 8:+58626555 MsaT044320.1:CDS
ATATATTATACCTTAGGAGT+TGG 0.560505 8:-58626565 None:intergenic
GTTCTGCCTTGCAGTCCAAG+AGG 0.596751 8:-58626364 None:intergenic
GATGTTCAACGTATTGAGCG+TGG 0.669371 8:+58626510 MsaT044320.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 58626357 58626575 58626357 ID=MsaG044320
Chr8 mRNA 58626357 58626575 58626357 ID=MsaT044320.1;Parent=MsaG044320
Chr8 exon 58626357 58626575 58626357 ID=MsaT044320.1.exon1;Parent=MsaT044320.1
Chr8 CDS 58626357 58626575 58626357 ID=cds.MsaT044320.1;Parent=MsaT044320.1
Gene Sequence

>MsaT044320.1

ATGGCAACCTCTTGGACTGCAAGGCAGAACAAACTGTTTGAACAAGCATTGGCTTTATATGATAGAGAAACTCCTGAAAGATGGCACAATGTTGCCAAAATGGTTGGAAAATCAGTTGAAGATGTTAAAAGTCACTATGAGATCCTCAAAGAAGATGTTCAACGTATTGAGCGTGGCCATATCCCTTTTCCTCGTTACAAAACTAACACCAACTCCTAA
Protein sequence

>MsaT044320.1

MATSWTARQNKLFEQALALYDRETPERWHNVAKMVGKSVEDVKSHYEILKEDVQRIERGHIPFPRYKTNTNS*