AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044351

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044351 MtrunA17_Chr8g0373571 68.156 358 15 7 1 277 1 340 1.05e-135 386
MsaG044351 MtrunA17_Chr8g0369161 33.515 367 130 13 9 267 17 377 8.09e-33 124
MsaG044351 MtrunA17_Chr7g0275321 32.537 335 138 13 30 277 32 365 1.13e-31 121
MsaG044351 MtrunA17_Chr1g0162721 30.508 295 156 9 19 276 19 301 3.92e-29 112
MsaG044351 MtrunA17_Chr8g0347741 31.831 355 145 15 1 265 1 348 6.86e-27 108
MsaG044351 MtrunA17_Chr5g0410531 30.831 373 140 11 3 264 10 375 1.92e-24 101
MsaG044351 MtrunA17_Chr4g0065261 43.750 80 44 1 197 276 91 169 2.82e-14 69.3
MsaG044351 MtrunA17_Chr3g0139301 45.070 71 39 0 197 267 124 194 3.67e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044351 AT4G36730.2 47.923 313 114 10 1 277 1 300 2.12e-74 230
MsaG044351 AT4G36730.1 47.239 326 99 11 1 277 1 302 2.84e-73 227
MsaG044351 AT2G35530.1 32.973 370 122 13 15 264 9 372 8.68e-34 127
MsaG044351 AT2G46270.1 31.894 301 137 9 30 265 30 327 1.34e-27 110
MsaG044351 AT2G46270.3 31.894 301 137 9 30 265 30 327 1.34e-27 110
MsaG044351 AT2G46270.2 34.892 278 136 10 30 265 30 304 9.77e-27 107
MsaG044351 AT2G46270.4 34.892 278 136 10 30 265 30 304 9.77e-27 107
MsaG044351 AT1G32150.1 33.708 356 135 14 4 264 13 362 1.67e-26 107
MsaG044351 AT4G01120.1 31.692 325 147 12 2 265 7 317 4.79e-26 105
MsaG044351 AT3G30530.1 43.038 79 43 1 199 277 81 157 5.78e-11 60.5

Find 79 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAATATTGAATGGACTTA+TGG 0.194622 8:-58925346 MsaT044351.1:CDS
ATCTGAAAACGATTCTATTA+AGG 0.259096 8:-58924319 MsaT044351.1:intron
CTTTCCACTCTCTCCTGCTT+TGG 0.297217 8:+58926052 None:intergenic
AGCAACAGTTGAGGCATAAT+AGG 0.367655 8:+58926588 None:intergenic
ACAAAAGAGGGTGGAGGCGC+TGG 0.372335 8:-58924406 MsaT044351.1:CDS
ATTTCTCAGTCATCTGTTCC+TGG 0.375045 8:-58925400 MsaT044351.1:intron
TCAAGGTTAGCAACTACTTC+AGG 0.401006 8:+58924016 None:intergenic
TATTTAGGCCTACTATAATC+CGG 0.403097 8:-58926626 MsaT044351.1:intron
TCAATATTAAGATTAGTTGC+AGG 0.411493 8:+58925358 None:intergenic
TTCCCAGGAGGGTACATTGC+AGG 0.414100 8:+58926401 None:intergenic
TGCATGGAGGTTGACCAATC+TGG 0.418724 8:+58927012 None:intergenic
ACTGTTGTTTGTTTCATTCA+AGG 0.431117 8:+58923999 None:intergenic
ATCGAGACAACAGGATTTCC+AGG 0.434892 8:+58925382 None:intergenic
CAACTAATCTTAATATTGAA+TGG 0.437227 8:-58925354 MsaT044351.1:CDS
GAGAGTGGAAAGGCAGGATC+AGG 0.441081 8:-58926041 MsaT044351.1:CDS
GGAAAGGCAGGATCAGGTTC+AGG 0.442394 8:-58926035 MsaT044351.1:CDS
GCAACAGTTGAGGCATAATA+GGG 0.445311 8:+58926589 None:intergenic
GTTGCAGGCATCGAGACAAC+AGG 0.464141 8:+58925373 None:intergenic
CAAAAGAGGGTGGAGGCGCT+GGG 0.468781 8:-58924405 MsaT044351.1:CDS
TTATGGAATGCATCTTCTGC+TGG 0.478101 8:-58925329 MsaT044351.1:intron
TGTATTTGCAGAAGTGCCCT+TGG 0.487321 8:+58926079 None:intergenic
GGGACCTGATGGAAAGGATA+AGG 0.491928 8:-58926114 MsaT044351.1:CDS
GAATCCTTATCCTTTCCATC+AGG 0.492059 8:+58926110 None:intergenic
GATCCTGCAATGTACCCTCC+TGG 0.499747 8:-58926404 MsaT044351.1:CDS
TAGGGAGGTGGAGCGGCTCC+CGG 0.502280 8:+58926607 None:intergenic
GGATCAGGTTCAGGCAATGA+TGG 0.509231 8:-58926026 MsaT044351.1:CDS
ATGCTAGCGATGAAACCAAC+AGG 0.509298 8:-58925872 MsaT044351.1:CDS
GCAGGAGAGAGTGGAAAGGC+AGG 0.510414 8:-58926047 MsaT044351.1:CDS
GTAATCTGTCATATACCTGT+TGG 0.523354 8:+58925857 None:intergenic
ATTTAGGCCTACTATAATCC+GGG 0.529964 8:-58926625 MsaT044351.1:intron
TAAGGATTCATCTAAAACCA+AGG 0.535406 8:-58926096 MsaT044351.1:CDS
AGGAGGGTACATTGCAGGAT+CGG 0.536165 8:+58926406 None:intergenic
GGAATCAGCTAGAAACAAGA+AGG 0.536581 8:-58925563 MsaT044351.1:CDS
GTTTGAAGGAAGGGACCTGA+TGG 0.537574 8:-58926125 MsaT044351.1:intron
ATCCTGCAATGTACCCTCCT+GGG 0.538664 8:-58926403 MsaT044351.1:CDS
TGACTAACCACTACCATGCT+AGG 0.543160 8:+58926365 None:intergenic
TGCTCATCCTAGCATGGTAG+TGG 0.548395 8:-58926372 MsaT044351.1:intron
AGTGCCAAAGCAGGAGAGAG+TGG 0.548817 8:-58926056 MsaT044351.1:CDS
TACAACCATCATATCCAGAT+TGG 0.548946 8:-58927026 MsaT044351.1:CDS
TAAAACATCTTCATCAACTC+AGG 0.549376 8:-58927152 MsaT044351.1:intron
GCAAATACAAGTGCCAAAGC+AGG 0.553074 8:-58926065 MsaT044351.1:CDS
ACATATAAGGATCACGGAGT+TGG 0.553334 8:+58926562 None:intergenic
CAGTGGTGACAGTGGTTCAG+AGG 0.554290 8:-58925904 MsaT044351.1:CDS
GTCGTGTAAACAGGAAGAGT+TGG 0.557803 8:-58924053 MsaT044351.1:intron
GGATATGATGGTTGTACCGT+TGG 0.566518 8:+58927033 None:intergenic
GAAGAGTTGGAACGGTTGTG+TGG 0.569549 8:-58924040 MsaT044351.1:CDS
GTTGACCAATCTGGATATGA+TGG 0.570626 8:+58927021 None:intergenic
AGATTGGTCAACCTCCATGC+AGG 0.576648 8:-58927010 MsaT044351.1:intron
CATATGTTCCATTACCTGCA+TGG 0.578688 8:+58926996 None:intergenic
GGATGAGCATACATATTCCC+AGG 0.579201 8:+58926386 None:intergenic
GCGGTCAAGACTACGCAAGC+AGG 0.579234 8:-58924994 MsaT044351.1:intron
AGTCTAATCGAGAGTCAGCT+AGG 0.579925 8:-58925016 MsaT044351.1:CDS
GCAAATGAGAGCACATGTGA+TGG 0.588499 8:+58925835 None:intergenic
AAGGAAGGGACCTGATGGAA+AGG 0.592851 8:-58926120 MsaT044351.1:intron
GTTGAGGCATAATAGGGAGG+TGG 0.593384 8:+58926595 None:intergenic
AAGAGTTGGAACGGTTGTGT+GGG 0.601166 8:-58924039 MsaT044351.1:CDS
AGCGGCTCCCGGATTATAGT+AGG 0.602344 8:+58926618 None:intergenic
GATGGTTGTACCGTTGGTGT+TGG 0.603834 8:+58927039 None:intergenic
GTGTGAAGAGCTACAAAAGA+GGG 0.605519 8:-58924418 MsaT044351.1:CDS
AGTGGTGACAGTGGTTCAGA+GGG 0.609578 8:-58925903 MsaT044351.1:CDS
AAGAGGGTGGAGGCGCTGGG+AGG 0.616742 8:-58924402 MsaT044351.1:CDS
GAATCAGCTAGAAACAAGAA+GGG 0.620018 8:-58925562 MsaT044351.1:CDS
TATGTATGCTCATCCTAGCA+TGG 0.624800 8:-58926378 MsaT044351.1:CDS
TGAAGAGCTACAAAAGAGGG+TGG 0.630738 8:-58924415 MsaT044351.1:CDS
AGTGTGAAGAGCTACAAAAG+AGG 0.632849 8:-58924419 MsaT044351.1:CDS
CAAAGCAGGAGAGAGTGGAA+AGG 0.636173 8:-58926051 MsaT044351.1:CDS
TCCTTAAACAGTGGTGACAG+TGG 0.636548 8:-58925912 MsaT044351.1:intron
AGAGCTACAAAAGAGGGTGG+AGG 0.641292 8:-58924412 MsaT044351.1:CDS
AAGGATTCATCTAAAACCAA+GGG 0.643002 8:-58926095 MsaT044351.1:CDS
GGCATAATAGGGAGGTGGAG+CGG 0.650234 8:+58926600 None:intergenic
AGTTGGTGAAGCAACAGTTG+AGG 0.658757 8:+58926579 None:intergenic
GTAAACAGGAAGAGTTGGAA+CGG 0.665668 8:-58924048 MsaT044351.1:intron
GAGCATACATATTCCCAGGA+GGG 0.682181 8:+58926390 None:intergenic
ACAGGAAGTACCAACACCAA+CGG 0.687437 8:-58927049 MsaT044351.1:intron
ACATACCATCAACAAGCATG+AGG 0.688310 8:+58925530 None:intergenic
ATGTTCCATTACCTGCATGG+AGG 0.690307 8:+58926999 None:intergenic
ACAGTTGAGGCATAATAGGG+AGG 0.694953 8:+58926592 None:intergenic
TGAGCATACATATTCCCAGG+AGG 0.720547 8:+58926389 None:intergenic
CTAATCGAGAGTCAGCTAGG+CGG 0.772559 8:-58925013 MsaT044351.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 58924012 58927209 58924012 ID=MsaG044351
Chr8 mRNA 58924012 58927209 58924012 ID=MsaT044351.1;Parent=MsaG044351
Chr8 exon 58924012 58924062 58924012 ID=MsaT044351.1.exon11;Parent=MsaT044351.1
Chr8 CDS 58924012 58924062 58924012 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58924320 58924445 58924320 ID=MsaT044351.1.exon10;Parent=MsaT044351.1
Chr8 CDS 58924320 58924445 58924320 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58924995 58925072 58924995 ID=MsaT044351.1.exon9;Parent=MsaT044351.1
Chr8 CDS 58924995 58925072 58924995 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58925330 58925413 58925330 ID=MsaT044351.1.exon8;Parent=MsaT044351.1
Chr8 CDS 58925330 58925413 58925330 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58925543 58925584 58925543 ID=MsaT044351.1.exon7;Parent=MsaT044351.1
Chr8 CDS 58925543 58925584 58925543 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58925830 58925923 58925830 ID=MsaT044351.1.exon6;Parent=MsaT044351.1
Chr8 CDS 58925830 58925923 58925830 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58926014 58926135 58926014 ID=MsaT044351.1.exon5;Parent=MsaT044351.1
Chr8 CDS 58926014 58926135 58926014 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58926373 58926430 58926373 ID=MsaT044351.1.exon4;Parent=MsaT044351.1
Chr8 CDS 58926373 58926430 58926373 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58926577 58926641 58926577 ID=MsaT044351.1.exon3;Parent=MsaT044351.1
Chr8 CDS 58926577 58926641 58926577 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58927011 58927067 58927011 ID=MsaT044351.1.exon2;Parent=MsaT044351.1
Chr8 CDS 58927011 58927067 58927011 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Chr8 exon 58927153 58927209 58927153 ID=MsaT044351.1.exon1;Parent=MsaT044351.1
Chr8 CDS 58927153 58927209 58927153 ID=cds.MsaT044351.1;Parent=MsaT044351.1
Gene Sequence

>MsaT044351.1

ATGGGGACTAAGGAGGATAGCACAACTAAGCCTTCTAAAACATCTTCATCAACTCAGGAAGTACCAACACCAACGGTACAACCATCATATCCAGATTGGTCAACCTCCATGCAGGCCTACTATAATCCGGGAGCCGCTCCACCTCCCTATTATGCCTCAACTGTTGCTTCACCAACTCCTTCCGATCCTGCAATGTACCCTCCTGGGAATATGTATGCTCATCCTAGCATGGTAGTGGGACCTGATGGAAAGGATAAGGATTCATCTAAAACCAAGGGCACTTCTGCAAATACAAGTGCCAAAGCAGGAGAGAGTGGAAAGGCAGGATCAGGTTCAGGCAATGATGGCTTTTCACATAGTGGTGACAGTGGTTCAGAGGGTTCATCTAATGCTAGCGATGAAACCAACAGGTATATGACAGATTACCATCACATGTGCTCTCATTTGCTTTTAGAATCAGCTAGAAACAAGAAGGGAAGCTTTGACCTCATGCTTTCATCTGTTCCTGGAAATCCTGTTGTCTCGATGCCTGCAACTAATCTTAATATTGAATGGACTTATGGAATGCATCTTCTGCTGGATGATCGTGAGTTGAAAAGACAGAAGAGAAAACAGTCTAATCGAGAGTCAGCTAGGCGGTCAAGACTACGCAAGCAGGCTGAGTGTGAAGAGCTACAAAAGAGGGTGGAGGCGCTGGGAGGTGAGAATCGAACTCTCAGAGAAGAGCTTCAAAAACTTTCTGAAGAGTGTGAGAAGCTTACATCTGAAAACGATTCTATTAAGGAAGAGTTGGAACGGTTGTGTGGGCCTGAAGTAGTTGCTAACCTTGAATGA
Protein sequence

>MsaT044351.1

MGTKEDSTTKPSKTSSSTQEVPTPTVQPSYPDWSTSMQAYYNPGAAPPPYYASTVASPTPSDPAMYPPGNMYAHPSMVVGPDGKDKDSSKTKGTSANTSAKAGESGKAGSGSGNDGFSHSGDSGSEGSSNASDETNRYMTDYHHMCSHLLLESARNKKGSFDLMLSSVPGNPVVSMPATNLNIEWTYGMHLLLDDRELKRQKRKQSNRESARRSRLRKQAECEELQKRVEALGGENRTLREELQKLSEECEKLTSENDSIKEELERLCGPEVVANLE*