AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044352


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044352 MtrunA17_Chr7g0225681 86.694 729 33 3 26 690 1 729 0.0 1115
MsaG044352 MtrunA17_Chr8g0373581 74.510 459 37 6 278 690 2 426 0.0 620
MsaG044352 MtrunA17_Chr8g0373591 95.911 269 11 0 26 294 1 269 6.60e-136 400
MsaG044352 MtrunA17_Chr8g0373591 70.000 120 32 4 196 314 45 161 6.97e-22 96.3
MsaG044352 MtrunA17_Chr1g0188151 94.565 92 5 0 599 690 1 92 9.73e-57 187
MsaG044352 MtrunA17_Chr1g0188141 82.828 99 8 1 26 124 1 90 1.11e-32 121
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044352 AT5G41020.1 54.770 283 127 1 405 687 302 583 1.11e-111 349

Find 127 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATAAACCCGTCCATTAT+AGG 0.233522 8:+58944928 None:intergenic
TCAATAAATTCAAGCTCTTC+AGG 0.254261 8:+58943206 None:intergenic
ATCATCATCTTCACATTCAT+TGG 0.267885 8:+58944067 None:intergenic
ACATTGCTTTCGGACATATA+TGG 0.292466 8:+58944809 None:intergenic
AACAAATGACCCACCATATT+GGG 0.299291 8:-58942722 MsaT044352.1:CDS
TTGATCTTCTCATCTTCTTC+GGG 0.322099 8:+58943425 None:intergenic
TTTGATCTTCTCATCTTCTT+CGG 0.324153 8:+58943424 None:intergenic
CTATATAGATTCTCATGGTT+TGG 0.339430 8:-58943387 MsaT044352.1:CDS
AAACATGCCGTGTCCTATAA+TGG 0.342941 8:-58944941 MsaT044352.1:CDS
GAACAAATGACCCACCATAT+TGG 0.350597 8:-58942723 MsaT044352.1:CDS
TATATAGATTCTCATGGTTT+GGG 0.352407 8:-58943386 MsaT044352.1:CDS
TCTCTATCTTTCAACAAGTT+GGG 0.365789 8:+58942642 None:intergenic
CTCTCTATCTTTCAACAAGT+TGG 0.365952 8:+58942641 None:intergenic
CAGATCAGTTACATTGCTTT+CGG 0.367004 8:+58944799 None:intergenic
TTATGTGTGCTTTCTTCACT+TGG 0.367181 8:+58944302 None:intergenic
TAGCCCTCTCAAGCAGATCT+AGG 0.372193 8:+58943021 None:intergenic
GATAGACGCTTTATATGCTT+TGG 0.375237 8:-58942823 MsaT044352.1:CDS
CTGATCTGCCTTTGATCAAA+AGG 0.395638 8:-58944784 MsaT044352.1:CDS
TGGCGGCATGCTCCCCAATA+TGG 0.396320 8:+58942709 None:intergenic
GGGAGACGAAGGTCTTGATA+TGG 0.410993 8:-58943366 MsaT044352.1:CDS
TTCCAACAGCCGCTAACCTC+AGG 0.416232 8:+58943323 None:intergenic
AGAAGCAATGAAGGTGATAA+TGG 0.417384 8:-58944548 MsaT044352.1:CDS
GTTTCATTATGTGCACAGTT+AGG 0.421260 8:+58943819 None:intergenic
GAGAGCAGTAGCTGACGCTT+TGG 0.427556 8:-58943153 MsaT044352.1:CDS
TGCTCATACTTTGTTAGAAA+AGG 0.429545 8:-58943249 MsaT044352.1:CDS
GTTGCGAGATAATATTTGTT+GGG 0.434220 8:-58942967 MsaT044352.1:CDS
TCATCATCTTCACATTCATT+GGG 0.442787 8:+58944068 None:intergenic
AGAGCAGTAGCTGACGCTTT+GGG 0.446113 8:-58943152 MsaT044352.1:CDS
GCTGCATGGAGGAGGTAGAC+TGG 0.446516 8:-58942794 MsaT044352.1:CDS
CTAAACGCAAACAAGTAACT+TGG 0.447239 8:-58943508 MsaT044352.1:intron
TATTCTTCTTCGTCTTGCCT+TGG 0.449662 8:+58943991 None:intergenic
CGCCAAGTCATTCATTGAGC+AGG 0.451714 8:-58942691 MsaT044352.1:CDS
CATTGGTTGCTAGTGGTGAT+TGG 0.452021 8:-58942869 MsaT044352.1:CDS
AATATTTGTTGGGAGGCAAT+TGG 0.463198 8:-58942957 MsaT044352.1:CDS
GTCACTGAAGTTAATGATCA+AGG 0.467385 8:-58943891 MsaT044352.1:CDS
CGTCGATACACACTCTCATA+AGG 0.471062 8:+58943272 None:intergenic
ACACTCTCATAAGGCCTCTC+AGG 0.477449 8:+58943281 None:intergenic
AAGAAGCACAAACTAATTGA+AGG 0.478439 8:-58944221 MsaT044352.1:CDS
ACTAGCTCCATGTTGCTCAT+AGG 0.479373 8:+58943178 None:intergenic
TGCTTTGGATGCTTGCTGCA+TGG 0.479442 8:-58942808 MsaT044352.1:CDS
AAAAGGAGGGAAGGTGTAAG+TGG 0.481580 8:-58943232 MsaT044352.1:CDS
TAGAAGCAATGGAGGTGAAG+AGG 0.481860 8:-58944171 MsaT044352.1:CDS
ACGGCTTATTGCGTGGGAAA+AGG 0.484774 8:-58943454 MsaT044352.1:CDS
TGTTGCGAGATAATATTTGT+TGG 0.489321 8:-58942968 MsaT044352.1:CDS
GGATGCTTGCTGCATGGAGG+AGG 0.492182 8:-58942802 MsaT044352.1:CDS
ACCCTGAGGTTAGCGGCTGT+TGG 0.493985 8:-58943325 MsaT044352.1:CDS
AAGTGAAGAAAGCACACATA+AGG 0.495960 8:-58944300 MsaT044352.1:CDS
AAGTGAAGAAAGTAAACATA+AGG 0.498172 8:-58944108 MsaT044352.1:CDS
TGAAGGTGATAATGGCGAGC+AGG 0.499793 8:-58944540 MsaT044352.1:CDS
GAGTGACGAAAGCAAACATA+AGG 0.503590 8:-58944489 MsaT044352.1:CDS
CTCTATCTTTCAACAAGTTG+GGG 0.504361 8:+58942643 None:intergenic
AGAAACTAATTGAGGAAGGT+GGG 0.510841 8:-58944022 MsaT044352.1:CDS
TGTAATGACTTTAGAAGCAA+TGG 0.511180 8:-58944182 MsaT044352.1:CDS
AAAAGCAATAAAAGTGAAGA+TGG 0.511576 8:-58944740 MsaT044352.1:CDS
CTGCTCAATGAATGACTTGG+CGG 0.516982 8:+58942692 None:intergenic
AAGAAACTAATTGAGGAAGG+TGG 0.519932 8:-58944023 MsaT044352.1:CDS
CGGCATGCTCCCCAATATGG+TGG 0.523841 8:+58942712 None:intergenic
AATCTACTTGAGCATAGGTC+TGG 0.523977 8:-58942768 MsaT044352.1:CDS
CTGCATGGAGGAGGTAGACT+GGG 0.526890 8:-58942793 MsaT044352.1:CDS
TCAGCATCACCTTCATTGCT+AGG 0.528214 8:+58943795 None:intergenic
TCATACTTTGTTAGAAAAGG+AGG 0.529507 8:-58943246 MsaT044352.1:CDS
AATGAAGAAGAAACTAATTG+AGG 0.530342 8:-58944030 MsaT044352.1:CDS
TTTGACTATATAGATTCTCA+TGG 0.532297 8:-58943392 MsaT044352.1:CDS
AAGAAGCGGAAGCTAATTGA+AGG 0.534553 8:-58944599 MsaT044352.1:CDS
CATATATGGATCGCCTCCAT+TGG 0.534788 8:+58944823 None:intergenic
GGAGGCAATTGGTCACAAGT+TGG 0.536501 8:-58942946 MsaT044352.1:CDS
AAAAGTGAAGATGGCGAGCA+AGG 0.538269 8:-58944731 MsaT044352.1:CDS
ATAGGTCTGGTGATGTGTGC+AGG 0.541652 8:-58942755 MsaT044352.1:CDS
ATGAGCAACATGGAGCTAGT+TGG 0.543870 8:-58943175 MsaT044352.1:CDS
TGCCGTGTCCTATAATGGAC+GGG 0.547590 8:-58944936 MsaT044352.1:CDS
AATCACAGGTTTGTTGTCAA+AGG 0.550299 8:+58942617 None:intergenic
TTTGGGTAAAAGTCGAGCTC+AGG 0.550583 8:-58943135 MsaT044352.1:CDS
AGTAACTTGGGCTGATGAAG+TGG 0.552578 8:-58943495 MsaT044352.1:CDS
AAGAAGAAACTAATTGAGGA+AGG 0.552685 8:-58944026 MsaT044352.1:CDS
AATGAAACTCCTAGCAATGA+AGG 0.554019 8:-58943804 MsaT044352.1:intron
AAGAGAACTGAAGGCGAAGA+GGG 0.556027 8:-58944851 MsaT044352.1:CDS
GACGGCTTTAGAAGCAATGA+AGG 0.559488 8:-58944557 MsaT044352.1:CDS
TGTTGTGACGGCTTATTGCG+TGG 0.561335 8:-58943461 MsaT044352.1:CDS
TGAGCAATTAACATCAACAT+TGG 0.568371 8:-58942886 MsaT044352.1:CDS
TAAACCTAGATCTGCTTGAG+AGG 0.570723 8:-58943025 MsaT044352.1:CDS
AGAAGCAATGGAGGTGAAGA+GGG 0.572295 8:-58944170 MsaT044352.1:CDS
TAGAAACCTCAACAAATCAC+AGG 0.579568 8:+58942603 None:intergenic
GAAGGTGATAATGGCGAGCA+GGG 0.580967 8:-58944539 MsaT044352.1:CDS
CATACTTTGTTAGAAAAGGA+GGG 0.581182 8:-58943245 MsaT044352.1:CDS
ACATCAACATTGGTTGCTAG+TGG 0.583463 8:-58942876 MsaT044352.1:CDS
AACAAACCTGTGATTTGTTG+AGG 0.586181 8:-58942609 None:intergenic
GGCATGCTCCCCAATATGGT+GGG 0.586599 8:+58942713 None:intergenic
GCGAGATAATATTTGTTGGG+AGG 0.587211 8:-58942964 MsaT044352.1:CDS
CATATAAAGCGTCTATCAAG+CGG 0.589919 8:+58942829 None:intergenic
GGCGAAGAGGGTGAAACCAA+TGG 0.590103 8:-58944839 MsaT044352.1:CDS
GAGGAGAAGTTATGTTGTGA+CGG 0.591020 8:-58943473 MsaT044352.1:CDS
AAATTACACCCTGAGGTTAG+CGG 0.591381 8:-58943332 MsaT044352.1:CDS
CTTTGTTAGAAAAGGAGGGA+AGG 0.592917 8:-58943241 MsaT044352.1:CDS
AGAAGCAATGATGATGAAGA+TGG 0.594055 8:-58944362 MsaT044352.1:CDS
ATGCCGTGTCCTATAATGGA+CGG 0.599001 8:-58944937 MsaT044352.1:CDS
AAACCTAGATCTGCTTGAGA+GGG 0.602034 8:-58943024 MsaT044352.1:CDS
TCCAACAGCCGCTAACCTCA+GGG 0.602654 8:+58943324 None:intergenic
TAAACGCAAACAAGTAACTT+GGG 0.603412 8:-58943507 MsaT044352.1:intron
CAACTTGTTGAAAGATAGAG+AGG 0.613256 8:-58942640 MsaT044352.1:CDS
AACTTGGGCTGATGAAGTGG+AGG 0.617904 8:-58943492 MsaT044352.1:CDS
GGGACAATCTACTTGAGCAT+AGG 0.618965 8:-58942773 MsaT044352.1:CDS
CAAGAGAACTGAAGGCGAAG+AGG 0.619279 8:-58944852 MsaT044352.1:CDS
GAAGGAAACACACTTGAGGA+AGG 0.622295 8:-58944581 MsaT044352.1:CDS
TCTCATGGTTTGGGAGACGA+AGG 0.623440 8:-58943377 MsaT044352.1:CDS
AACCTGCTCAATGAATGACT+TGG 0.625939 8:+58942689 None:intergenic
GTGATGTGTGCAGGAAACGA+TGG 0.634053 8:-58942746 MsaT044352.1:CDS
GAAGGTGGGAATCGTGACCA+AGG 0.636683 8:-58944008 MsaT044352.1:CDS
CTGCCTTTGATCAAAAGGAA+TGG 0.637174 8:-58944779 MsaT044352.1:CDS
ACAAATGACCCACCATATTG+GGG 0.637544 8:-58942721 MsaT044352.1:CDS
GAAAAGACCTATGAGCAACA+TGG 0.638568 8:-58943185 MsaT044352.1:CDS
GTTGTGACGGCTTATTGCGT+GGG 0.642851 8:-58943460 MsaT044352.1:CDS
AACCCGTCCATTATAGGACA+CGG 0.642991 8:+58944934 None:intergenic
TTTGGATGCTTGCTGCATGG+AGG 0.643384 8:-58942805 MsaT044352.1:CDS
TGATGTGTGCAGGAAACGAT+GGG 0.644218 8:-58942745 MsaT044352.1:CDS
AATGAATGTGAAGATGATGA+TGG 0.645642 8:-58944065 MsaT044352.1:CDS
AATTGAAGGAAACACACTGA+AGG 0.653637 8:-58944399 MsaT044352.1:CDS
AATGACTTTAGAAGCAATGG+AGG 0.658645 8:-58944179 MsaT044352.1:CDS
AACACACTTGAGGAAGGTGA+CGG 0.661228 8:-58944575 MsaT044352.1:CDS
AAATGTCAAATTACACCCTG+AGG 0.666420 8:-58943339 MsaT044352.1:CDS
AAAAGGACATTGGTCCCAAG+AGG 0.666914 8:-58943072 MsaT044352.1:CDS
AATTGAAGGAAACACACTTG+AGG 0.669341 8:-58944585 MsaT044352.1:CDS
TGTGAAGATGATGATGGCCA+AGG 0.683186 8:-58944059 MsaT044352.1:CDS
GAGCTCAGGTAAAAGATGCG+TGG 0.687675 8:-58943121 MsaT044352.1:CDS
GAAGAGGGTGAAACCAATGG+AGG 0.700449 8:-58944836 MsaT044352.1:CDS
GAATGTGAAGCTGATGACCA+AGG 0.711673 8:-58944443 MsaT044352.1:CDS
AATGAATGTGAAGATGACCA+AGG 0.719006 8:-58944257 MsaT044352.1:CDS
GAATGTGAAGATGATGACCA+AGG 0.731540 8:-58944635 MsaT044352.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 58942611 58944972 58942611 ID=MsaG044352
Chr8 mRNA 58942611 58944972 58942611 ID=MsaT044352.1;Parent=MsaG044352
Chr8 exon 58942611 58943515 58942611 ID=MsaT044352.1.exon2;Parent=MsaT044352.1
Chr8 CDS 58942611 58943515 58942611 ID=cds.MsaT044352.1;Parent=MsaT044352.1
Chr8 exon 58943805 58944972 58943805 ID=MsaT044352.1.exon1;Parent=MsaT044352.1
Chr8 CDS 58943805 58944972 58943805 ID=cds.MsaT044352.1;Parent=MsaT044352.1
Gene Sequence

>MsaT044352.1

ATGTATTATAAACATGCCGTGTCCTATAATGGACGGGTTTATTTTGCAGTCATTGAAGAGGGGCACAAAACGAAGATGAAGACTCCGAGCAAGAAAAACAAGAGAACTGAAGGCGAAGAGGGTGAAACCAATGGAGGCGATCCATATATGTCCGAAAGCAATGTAACTGATCTGCCTTTGATCAAAAGGAATGGTGATCAACATTTGATCAAAAGCAATAAAAGTGAAGATGGCGAGCAAGGCAAAAAGAAAAAGAAAAAGAAGAAACTGAGTGAAGAAAGCAATCATAAGAAGTATAATGAGTTCAATAGCAATGAATGTGAAGATGATGACCAAGGAAAGAAGATGAAAAAGAAGCGGAAGCTAATTGAAGGAAACACACTTGAGGAAGGTGACGGCTTTAGAAGCAATGAAGGTGATAATGGCGAGCAGGGAAAGAAGAAAAAGAAAAAGAAGAAATTGAGTGACGAAAGCAAACATAAGGAGTATAACGAGTTCAAAAGTAATGAATGTGAAGCTGATGACCAAGGAAAGAAGATGAAAAAGAAGCTAATTGAAGGAAACACACTGAAGGAATGTAATGACTTCAGAAGCAATGATGATGAAGATGGTGAGCGAGAGAAAAAGATAAAGAAAAAGAAGAAGAAACCAAGTGAAGAAAGCACACATAAGGAGTATAGTGAGTTCAAAAGCAATGAATGTGAAGATGACCAAGGAAAAAAGACGAAAAAGAAGCACAAACTAATTGAAGGAAGCACACGTACTGAGTGTAATGACTTTAGAAGCAATGGAGGTGAAGAGGGTGAGCAAGACAAGAAGATGAAGAAAAAGAAGAAGAAACTAAGTGAAGAAAGTAAACATAAGGAGTATAGCAAGTTTGAACCCAATGAATGTGAAGATGATGATGGCCAAGGAAAGAAAATGAAGAAGAAACTAATTGAGGAAGGTGGGAATCGTGACCAAGGCAAGACGAAGAAGAATAAACCGTGTCAGAAAAAGAAAAGTAAGGAGAACAATGATTTTAATAGCAAAGAAGGTGATGCTGATACTTGTTTCTCTGTCACTGAAGTTAATGATCAAGGCAAAAAGATGAAGAAAAAAAAGAAAGCAAAGACAGTTACAAGCGAGTCTCCTAACTGTGCACATAATGAAACTCCTAGCAATGAAGTAACTTGGGCTGATGAAGTGGAGGAGAAGTTATGTTGTGACGGCTTATTGCGTGGGAAAAGGTATACACCCGAAGAAGATGAGAAGATCAAAGCAGCTGTTTTTGACTATATAGATTCTCATGGTTTGGGAGACGAAGGTCTTGATATGGTTCTAAATGTCAAATTACACCCTGAGGTTAGCGGCTGTTGGAAAGAAATTGCAAAAAGCATACCTGAGAGGCCTTATGAGAGTGTGTATCGACGTGCTCATACTTTGTTAGAAAAGGAGGGAAGGTGTAAGTGGACACCTGAAGAGCTTGAATTTATTGAAAAGACCTATGAGCAACATGGAGCTAGTTGGAGAGCAGTAGCTGACGCTTTGGGTAAAAGTCGAGCTCAGGTAAAAGATGCGTGGCGCAGATTGAAATATACCAAAGCAAAAAAAGGACATTGGTCCCAAGAGGAGTATCAAAATTTATTTAACTTAGTAAACCTAGATCTGCTTGAGAGGGCTAAAGAGCCTTATAAAAAATCACAACATGGTATGTTGCGAGATAATATTTGTTGGGAGGCAATTGGTCACAAGTTGGAGACTCGAAACAGTGCATTTTGCTGCAAGAAGTGGTATGAGCAATTAACATCAACATTGGTTGCTAGTGGTGATTGGTGTGATACTGATGACTTCCGCTTGATAGACGCTTTATATGCTTTGGATGCTTGCTGCATGGAGGAGGTAGACTGGGACAATCTACTTGAGCATAGGTCTGGTGATGTGTGCAGGAAACGATGGGAACAAATGACCCACCATATTGGGGAGCATGCCGCCAAGTCATTCATTGAGCAGGTTGAAGTTCTTGCAAAGAGATTCTGCCCCAACTTGTTGAAAGATAGAGAGGCCTTTGACAACAAACCTGTGATTTGTTGA

Protein sequence

>MsaT044352.1

MYYKHAVSYNGRVYFAVIEEGHKTKMKTPSKKNKRTEGEEGETNGGDPYMSESNVTDLPLIKRNGDQHLIKSNKSEDGEQGKKKKKKKKLSEESNHKKYNEFNSNECEDDDQGKKMKKKRKLIEGNTLEEGDGFRSNEGDNGEQGKKKKKKKKLSDESKHKEYNEFKSNECEADDQGKKMKKKLIEGNTLKECNDFRSNDDEDGEREKKIKKKKKKPSEESTHKEYSEFKSNECEDDQGKKTKKKHKLIEGSTRTECNDFRSNGGEEGEQDKKMKKKKKKLSEESKHKEYSKFEPNECEDDDGQGKKMKKKLIEEGGNRDQGKTKKNKPCQKKKSKENNDFNSKEGDADTCFSVTEVNDQGKKMKKKKKAKTVTSESPNCAHNETPSNEVTWADEVEEKLCCDGLLRGKRYTPEEDEKIKAAVFDYIDSHGLGDEGLDMVLNVKLHPEVSGCWKEIAKSIPERPYESVYRRAHTLLEKEGRCKWTPEELEFIEKTYEQHGASWRAVADALGKSRAQVKDAWRRLKYTKAKKGHWSQEEYQNLFNLVNLDLLERAKEPYKKSQHGMLRDNICWEAIGHKLETRNSAFCCKKWYEQLTSTLVASGDWCDTDDFRLIDALYALDACCMEEVDWDNLLEHRSGDVCRKRWEQMTHHIGEHAAKSFIEQVEVLAKRFCPNLLKDREAFDNKPVIC*