AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044373

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044373 MtrunA17_Chr8g0373781 96.262 535 16 1 1 535 1 531 0.0 1069
MsaG044373 MtrunA17_Chr1g0154501 57.757 535 159 8 1 535 1 468 0.0 582
MsaG044373 MtrunA17_Chr5g0436051 65.416 373 119 3 163 535 253 615 3.59e-174 505
MsaG044373 MtrunA17_Chr5g0403261 66.845 374 84 4 1 342 1 366 7.01e-173 492
MsaG044373 MtrunA17_Chr3g0112601 57.271 447 171 6 105 535 70 512 1.67e-166 482
MsaG044373 MtrunA17_Chr5g0403301 73.355 304 57 2 232 535 27 306 1.01e-151 436
MsaG044373 MtrunA17_Chr5g0403271 80.769 156 27 1 380 535 1 153 2.15e-77 240
MsaG044373 MtrunA17_Chr5g0403291 63.441 93 33 1 156 247 374 466 1.05e-34 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044373 AT4G36860.1 68.327 562 142 5 1 535 1 553 0.0 735
MsaG044373 AT4G36860.3 65.641 585 143 5 1 535 1 577 0.0 726
MsaG044373 AT4G36860.2 66.962 451 119 4 1 424 1 448 0.0 587
MsaG044373 AT1G19270.1 53.097 565 201 8 1 535 1 531 0.0 573
MsaG044373 AT1G19270.2 53.097 565 201 8 1 535 1 531 0.0 573
MsaG044373 AT2G39830.3 58.797 449 156 7 111 535 75 518 0.0 520
MsaG044373 AT2G39830.1 66.220 373 121 3 163 535 160 527 1.24e-179 516
MsaG044373 AT2G39830.2 65.691 376 121 4 163 535 160 530 2.69e-177 510
MsaG044373 AT5G66610.1 44.250 513 249 11 28 529 65 551 2.17e-133 399
MsaG044373 AT2G39830.4 65.233 279 94 3 163 440 160 436 5.69e-133 394
MsaG044373 AT5G66610.3 48.592 426 197 8 111 529 14 424 4.86e-130 386
MsaG044373 AT5G66620.1 45.010 491 244 10 44 528 170 640 7.57e-129 390
MsaG044373 AT5G66620.3 45.010 491 244 10 44 528 161 631 1.05e-128 390
MsaG044373 AT5G66620.4 45.010 491 244 10 44 528 161 631 1.05e-128 390
MsaG044373 AT5G66610.2 42.037 540 249 12 28 529 65 578 2.63e-127 384
MsaG044373 AT5G17890.1 51.309 382 174 6 159 535 1234 1608 1.84e-112 366
MsaG044373 AT5G66630.1 47.989 373 176 7 159 528 341 698 4.96e-109 341
MsaG044373 AT5G66640.5 40.272 514 236 18 33 528 10 470 1.49e-100 312
MsaG044373 AT5G66640.1 48.023 354 173 8 182 528 89 438 1.89e-100 311
MsaG044373 AT5G66640.7 48.023 354 173 8 182 528 89 438 1.89e-100 311
MsaG044373 AT5G66640.4 48.023 354 173 8 182 528 120 469 5.47e-100 310
MsaG044373 AT5G66640.3 49.691 324 154 7 212 528 10 331 5.13e-96 295
MsaG044373 AT5G66640.6 36.965 514 249 19 33 528 10 466 2.34e-83 267
MsaG044373 AT5G66640.2 43.220 354 186 9 182 528 120 465 4.69e-83 266
MsaG044373 AT5G66620.2 38.810 353 191 9 44 390 161 494 1.34e-72 240

Find 119 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCCTTATAGCATAATTTA+TGG 0.189171 8:+59215508 None:intergenic
ATCCTCGTCCTGAATGTTTA+TGG 0.190323 8:+59216900 None:intergenic
AGTATAGAGCACATCCTTTC+TGG 0.193526 8:-59214975 MsaT044373.1:CDS
CATGCTTGGCTTAGACTTAA+AGG 0.236159 8:-59213830 MsaT044373.1:intron
GTGTCTCGATTCGGCTATTA+TGG 0.241072 8:-59214707 MsaT044373.1:CDS
AACCAGGCAGTGCTCAAGTA+TGG 0.287660 8:-59213452 MsaT044373.1:CDS
CATATTCCTTCTTCAACTTC+AGG 0.292654 8:+59213701 None:intergenic
CATCGACGTATCAGCCTAAA+AGG 0.297552 8:+59214201 None:intergenic
CGAGGATACACCGGGAAATA+TGG 0.299410 8:-59216884 MsaT044373.1:CDS
GTCCTCTGGTTTCAGGTAAA+TGG 0.302701 8:+59214410 None:intergenic
AAGCAAAGTCCTCTGGTTTC+AGG 0.302816 8:+59214403 None:intergenic
GCAGCAAATTCCTATGCTTT+TGG 0.308444 8:-59214614 MsaT044373.1:CDS
AACTTCCGGTCATTCGGATA+TGG 0.336770 8:+59213411 None:intergenic
TCATTACAGTATTCCATGTC+TGG 0.337779 8:-59215543 MsaT044373.1:intron
GGCGAAAAGAGGTTTGTAAA+AGG 0.349058 8:+59216114 None:intergenic
ACCAAGTTCCTTCTCAAAAT+CGG 0.357058 8:+59213541 None:intergenic
TGATGATGCTTCTTCACTTC+CGG 0.357113 8:+59213631 None:intergenic
TTACCATAAATTATGCTATA+AGG 0.387755 8:-59215511 MsaT044373.1:CDS
GTTCGAACCCTTTAGAAACT+TGG 0.408812 8:+59216922 None:intergenic
TGCTTCTTCACTTCCGGAGC+CGG 0.411249 8:+59213637 None:intergenic
TTAGATTCAGAGATCTATTC+CGG 0.411303 8:-59213656 MsaT044373.1:CDS
GGTTCGAACCATAAACATTC+AGG 0.417018 8:-59216908 MsaT044373.1:CDS
CTGGCTTACCAAGTTTCTAA+AGG 0.419265 8:-59216930 MsaT044373.1:CDS
GTTACAGAGGAAGATCATAA+AGG 0.421978 8:-59216653 MsaT044373.1:CDS
GTGAGCTATCTGTCTCAATC+TGG 0.433359 8:+59213504 None:intergenic
ATGGAAAACTTCCGGTCATT+CGG 0.433899 8:+59213405 None:intergenic
TCGCATAGTTACCTGTATAC+AGG 0.438440 8:+59216093 None:intergenic
GCTGGCTGCAATGCCGAGAT+TGG 0.442433 8:-59215813 MsaT044373.1:CDS
TTCTGACAAGCAAAGTCCTC+TGG 0.446916 8:+59214396 None:intergenic
TGGCTTACCAAGTTTCTAAA+GGG 0.451933 8:-59216929 MsaT044373.1:CDS
TTACAGAGGAAGATCATAAA+GGG 0.469977 8:-59216652 MsaT044373.1:CDS
GAAAATAAGATTGTATGGGC+TGG 0.473993 8:-59216949 None:intergenic
ATTTCATTATGGAAAACTTC+CGG 0.475409 8:+59213397 None:intergenic
TTCCTCTACATCTTCAAAGA+AGG 0.477132 8:-59213573 MsaT044373.1:CDS
CGAACCTCGTAATCAGTGAT+TGG 0.478079 8:+59215717 None:intergenic
CATCTTCAAAGAAGGGTAAA+CGG 0.478692 8:-59213565 MsaT044373.1:CDS
AAGGCCATACAAAACAAGTA+TGG 0.479856 8:+59214167 None:intergenic
AGGAAGATGAACAACTTGCC+AGG 0.481242 8:-59216200 MsaT044373.1:CDS
CTCATGAAATGATGCATGCT+TGG 0.482818 8:-59213844 MsaT044373.1:CDS
TGATTGGAAGTTTGCAAGCA+TGG 0.482821 8:+59215733 None:intergenic
CAAACTTTCTTGAATTGCCC+TGG 0.485539 8:+59216182 None:intergenic
CCATGTTGCTAAAATTCAAC+AGG 0.488050 8:-59216256 MsaT044373.1:CDS
CGGTAGCCTGCTCCTATACT+TGG 0.493239 8:+59214246 None:intergenic
GTATTTCAAGATAGAGAGGT+TGG 0.497395 8:+59214671 None:intergenic
TCAGAGATCTATTCCGGCTC+CGG 0.497689 8:-59213650 MsaT044373.1:CDS
TGAGTTGCATGGGAGCTGTA+TGG 0.499390 8:-59215776 MsaT044373.1:CDS
TTATAGCATAATTTATGGTA+AGG 0.500224 8:+59215513 None:intergenic
TCTGTCGTGTCCATATTTCC+CGG 0.502358 8:+59216874 None:intergenic
GTGCCATCGCGCTCGTGTGA+TGG 0.502362 8:+59214937 None:intergenic
GTGTCTTGAGTGTCTCGATT+CGG 0.506058 8:-59214716 MsaT044373.1:CDS
ACAGATAGCTCACCAGCCTA+TGG 0.509351 8:-59213494 MsaT044373.1:CDS
GCCCATACTTGAGCACTGCC+TGG 0.512523 8:+59213450 None:intergenic
ATATGGACACGACAGAGATT+CGG 0.513953 8:-59216867 MsaT044373.1:CDS
TATATTTATAGGTTACTGAC+AGG 0.516589 8:-59213878 MsaT044373.1:intron
TGGATTTAGAGAGGGTAACC+AGG 0.516593 8:-59213468 MsaT044373.1:CDS
CAGATCCCACAAAATTCAGC+TGG 0.520653 8:-59215006 MsaT044373.1:CDS
TTGTTGTAGCTGTCAAAGAA+TGG 0.521248 8:-59214908 MsaT044373.1:CDS
CATTACAGTATTCCATGTCT+GGG 0.527623 8:-59215542 MsaT044373.1:intron
ATAGAATCAGTTTGAGATGG+AGG 0.527868 8:+59216144 None:intergenic
AAACCTTCCATGGCCAATCT+CGG 0.530954 8:+59215800 None:intergenic
AGGAGACCAAGTATAGGAGC+AGG 0.531668 8:-59214252 MsaT044373.1:CDS
CTAAATCCATCTCCATAGGC+TGG 0.535720 8:+59213482 None:intergenic
AGCTCACCAGCCTATGGAGA+TGG 0.537937 8:-59213488 MsaT044373.1:CDS
CTGAGGCCTGAAGTTGAAGA+AGG 0.538408 8:-59213707 MsaT044373.1:CDS
CATGCATCATTTCATGAGCT+AGG 0.540468 8:+59213849 None:intergenic
ACCTGTATACAGGCGAAAAG+AGG 0.540632 8:+59216103 None:intergenic
AATATAGAATCAGTTTGAGA+TGG 0.540843 8:+59216141 None:intergenic
TCTTGCAAACATCACACCTT+GGG 0.542837 8:+59215478 None:intergenic
TGCTCAAGTATGGGCTTAGA+CGG 0.544906 8:-59213442 MsaT044373.1:CDS
CATTCAGGACGAGGATACAC+CGG 0.549451 8:-59216893 MsaT044373.1:CDS
AGAAGGAATATGCCAAGTCT+TGG 0.551472 8:-59213690 MsaT044373.1:CDS
ACCACATATGAGCCAAGACT+TGG 0.555232 8:+59213678 None:intergenic
GCTGATGTTGAACAAAATGA+AGG 0.556484 8:-59216282 MsaT044373.1:CDS
ATGCCGAGATTGGCCATGGA+AGG 0.556967 8:-59215803 MsaT044373.1:CDS
ACCAGGCAGTGCTCAAGTAT+GGG 0.557753 8:-59213451 MsaT044373.1:CDS
ACCCTTCTTTGAAGATGTAG+AGG 0.559513 8:+59213571 None:intergenic
TCCTCTACATCTTCAAAGAA+GGG 0.564606 8:-59213572 MsaT044373.1:CDS
GCCAAGTCTTGGCTCATATG+TGG 0.565359 8:-59213679 MsaT044373.1:CDS
GAATCAGTTTGAGATGGAGG+AGG 0.568408 8:+59216147 None:intergenic
CTTGACCATATCCGAATGAC+CGG 0.569122 8:-59213416 MsaT044373.1:CDS
TGTGCTTGCAGAATCTGTGC+TGG 0.570167 8:-59215831 MsaT044373.1:intron
CTCTCTAAATCCATCTCCAT+AGG 0.573587 8:+59213478 None:intergenic
ATCTTGCAAACATCACACCT+TGG 0.577586 8:+59215477 None:intergenic
TTCTTTGAAGATGTAGAGGA+AGG 0.578442 8:+59213575 None:intergenic
TCTTTCAGAGGTTACAGAGG+AGG 0.581657 8:-59216675 MsaT044373.1:CDS
AAGTATCTGTTGCTTGATGA+CGG 0.585206 8:-59214748 MsaT044373.1:CDS
TCTTGTATTTCAAGATAGAG+AGG 0.585747 8:+59214667 None:intergenic
ATTCAAGAAAGTTTGAGCGT+TGG 0.587908 8:-59216174 MsaT044373.1:CDS
TGCAATGCCGAGATTGGCCA+TGG 0.592397 8:-59215807 MsaT044373.1:CDS
AATTGCGATCTCTCTTTCAG+AGG 0.593587 8:-59216687 MsaT044373.1:CDS
TGTCTCTCAACCAAAAGCAT+AGG 0.601813 8:+59214604 None:intergenic
ACAAGCACTAAATGAAGCCA+TGG 0.603423 8:-59214584 MsaT044373.1:CDS
CTCTCTTTCAGAGGTTACAG+AGG 0.604714 8:-59216678 MsaT044373.1:CDS
CGACAGGCTATGGGAATCTG+AGG 0.609358 8:-59213724 MsaT044373.1:intron
AGATTCTGAATCTGAAGATG+AGG 0.610392 8:-59216343 MsaT044373.1:CDS
ATTCAGGACGAGGATACACC+GGG 0.612301 8:-59216892 MsaT044373.1:CDS
TCATCTTCCTCGTCATCAAG+TGG 0.612402 8:+59216312 None:intergenic
ATAAGGAGCTGCATCACCCA+AGG 0.612966 8:-59215494 MsaT044373.1:CDS
CTATGGAGATGGATTTAGAG+AGG 0.615579 8:-59213477 MsaT044373.1:CDS
CTGATGTTGAACAAAATGAA+GGG 0.615941 8:-59216281 MsaT044373.1:CDS
GTAAGGACGATTCCCAGACA+TGG 0.624532 8:+59215530 None:intergenic
GGAAGATGAACAACTTGCCA+GGG 0.625754 8:-59216199 MsaT044373.1:CDS
CAGTTATCATATCGATGACA+CGG 0.628026 8:+59214226 None:intergenic
AACCATAAACATTCAGGACG+AGG 0.628809 8:-59216902 MsaT044373.1:CDS
TGTTGACGAAGTTCAACTTG+AGG 0.636560 8:-59216220 MsaT044373.1:CDS
TATGGAGATGGATTTAGAGA+GGG 0.638780 8:-59213476 MsaT044373.1:CDS
TATCAGAAGGATACCTAGGA+AGG 0.638978 8:+59214148 None:intergenic
GGTTACAGAGGAGGTTACAG+AGG 0.639025 8:-59216666 MsaT044373.1:CDS
GATGTGCTCTATACTCAATG+AGG 0.649586 8:+59214983 None:intergenic
ACTTCCAATCACTGATTACG+AGG 0.658587 8:-59215721 MsaT044373.1:intron
GCACTAAATGAAGCCATGGA+GGG 0.659884 8:-59214580 MsaT044373.1:CDS
TGTCCATCACACGAGCGCGA+TGG 0.661781 8:-59214940 MsaT044373.1:CDS
AGCACTAAATGAAGCCATGG+AGG 0.665183 8:-59214581 MsaT044373.1:CDS
TTGTAGCTGTCAAAGAATGG+AGG 0.683641 8:-59214905 MsaT044373.1:intron
GTTGTGTCCACTTGATGACG+AGG 0.696227 8:-59216319 MsaT044373.1:CDS
CACCATTTACCTGAAACCAG+AGG 0.704277 8:-59214412 MsaT044373.1:CDS
TGATGTTGAACAAAATGAAG+GGG 0.704807 8:-59216280 MsaT044373.1:CDS
CTTTGACAGCTACAACAACG+AGG 0.719508 8:+59214913 None:intergenic
GGATAATCATGATAATTCCG+CGG 0.721301 8:-59216846 MsaT044373.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 59213403 59216957 59213403 ID=MsaG044373
Chr8 mRNA 59213403 59216957 59213403 ID=MsaT044373.1;Parent=MsaG044373
Chr8 exon 59213403 59213740 59213403 ID=MsaT044373.1.exon11;Parent=MsaT044373.1
Chr8 CDS 59213403 59213740 59213403 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59213831 59213889 59213831 ID=MsaT044373.1.exon10;Parent=MsaT044373.1
Chr8 CDS 59213831 59213889 59213831 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59214162 59214280 59214162 ID=MsaT044373.1.exon9;Parent=MsaT044373.1
Chr8 CDS 59214162 59214280 59214162 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59214378 59214443 59214378 ID=MsaT044373.1.exon8;Parent=MsaT044373.1
Chr8 CDS 59214378 59214443 59214378 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59214570 59214782 59214570 ID=MsaT044373.1.exon7;Parent=MsaT044373.1
Chr8 CDS 59214570 59214782 59214570 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59214906 59215025 59214906 ID=MsaT044373.1.exon6;Parent=MsaT044373.1
Chr8 CDS 59214906 59215025 59214906 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59215473 59215556 59215473 ID=MsaT044373.1.exon5;Parent=MsaT044373.1
Chr8 CDS 59215473 59215556 59215473 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59215722 59215842 59215722 ID=MsaT044373.1.exon4;Parent=MsaT044373.1
Chr8 CDS 59215722 59215842 59215722 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59216105 59216369 59216105 ID=MsaT044373.1.exon3;Parent=MsaT044373.1
Chr8 CDS 59216105 59216369 59216105 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59216639 59216750 59216639 ID=MsaT044373.1.exon2;Parent=MsaT044373.1
Chr8 CDS 59216639 59216750 59216639 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Chr8 exon 59216847 59216957 59216847 ID=MsaT044373.1.exon1;Parent=MsaT044373.1
Chr8 CDS 59216847 59216957 59216847 ID=cds.MsaT044373.1;Parent=MsaT044373.1
Gene Sequence

>MsaT044373.1

ATGGGCTGGCTTACCAAGTTTCTAAAGGGTTCGAACCATAAACATTCAGGACGAGGATACACCGGGAAATATGGACACGACAGAGATTCGGATAATCATGATAATTCCGCGGATGATTTGAATGATTTTGAGAGAGAAGAAATTGATCGTGCAATTGCGATCTCTCTTTCAGAGGTTACAGAGGAGGTTACAGAGGAAGATCATAAAGGGAAAAAAGTTATCGAAGAAGATTCTGAATCTGAAGATGAGGAGTTGTGTCCACTTGATGACGAGGAAGATGAGCATGTTGCTGATGTTGAACAAAATGAAGGGGACCATGTTGCTAAAATTCAACAGGAAGAAGAAGAAAATGTTGACGAAGTTCAACTTGAGGAAGATGAACAACTTGCCAGGGCAATTCAAGAAAGTTTGAGCGTTGGTTCACCTCCTCCATCTCAAACTGATTCTATATTTCAACCTTTTACAAACCTCTTTTCGCCTGTATACAGAATCTGTGCTGGCTGCAATGCCGAGATTGGCCATGGAAGGTTTTTGAGTTGCATGGGAGCTGTATGGCATCCAGAATGTTTTTGTTGCCATGCTTGCAAACTTCCAATCACTGATTACGAGTATTCCATGTCTGGGAATCGTCCTTACCATAAATTATGCTATAAGGAGCTGCATCACCCAAGGTGTGATGTTTGCAAGATCTTTATCCCACAAAATTCAGCTGGCCTCATTGAGTATAGAGCACATCCTTTCTGGCTACAAAAATACTGTCCATCACACGAGCGCGATGGCACTCCTCGTTGTTGTAGCTGTCAAAGAATGGAGTCAACGGATACAAAGTATCTGTTGCTTGATGACGGTCGAAAACTGTGTCTTGAGTGTCTCGATTCGGCTATTATGGATACTCATGAATGCCAACCTCTCTATCTTGAAATACAAGAATTTTACGAAGGTTTGCATATGAAAATAGAGCAGCAAATTCCTATGCTTTTGGTTGAGAGACAAGCACTAAATGAAGCCATGGAGGGCGAAAAGAATGGTCATAATCACCATTTACCTGAAACCAGAGGACTTTGCTTGTCAGAAGAGCAAACTGTCCCCACTATTTTAAGGAGACCAAGTATAGGAGCAGGCTACCGTGTCATCGATATGATAACTGAGCCTTTTAGGCTGATACGTCGATGTGAAGTGACAGCCATACTTGTTTTGTATGGCCTTCCTAGGTTACTGACAGGATCAATCCTAGCTCATGAAATGATGCATGCTTGGCTTAGACTTAAAGGCTATGGGAATCTGAGGCCTGAAGTTGAAGAAGGAATATGCCAAGTCTTGGCTCATATGTGGTTAGATTCAGAGATCTATTCCGGCTCCGGAAGTGAAGAAGCATCATCATCTTCATCTTCATCTTCGTCGTCTTCATCATCACCTTCCTCTACATCTTCAAAGAAGGGTAAACGGTCCGATTTTGAGAAGGAACTTGGTAAATTTTTTAAACACCAGATTGAGACAGATAGCTCACCAGCCTATGGAGATGGATTTAGAGAGGGTAACCAGGCAGTGCTCAAGTATGGGCTTAGACGGACTCTTGACCATATCCGAATGACCGGAAGTTTTCCATAA
Protein sequence

>MsaT044373.1

MGWLTKFLKGSNHKHSGRGYTGKYGHDRDSDNHDNSADDLNDFEREEIDRAIAISLSEVTEEVTEEDHKGKKVIEEDSESEDEELCPLDDEEDEHVADVEQNEGDHVAKIQQEEEENVDEVQLEEDEQLARAIQESLSVGSPPPSQTDSIFQPFTNLFSPVYRICAGCNAEIGHGRFLSCMGAVWHPECFCCHACKLPITDYEYSMSGNRPYHKLCYKELHHPRCDVCKIFIPQNSAGLIEYRAHPFWLQKYCPSHERDGTPRCCSCQRMESTDTKYLLLDDGRKLCLECLDSAIMDTHECQPLYLEIQEFYEGLHMKIEQQIPMLLVERQALNEAMEGEKNGHNHHLPETRGLCLSEEQTVPTILRRPSIGAGYRVIDMITEPFRLIRRCEVTAILVLYGLPRLLTGSILAHEMMHAWLRLKGYGNLRPEVEEGICQVLAHMWLDSEIYSGSGSEEASSSSSSSSSSSSSPSSTSSKKGKRSDFEKELGKFFKHQIETDSSPAYGDGFREGNQAVLKYGLRRTLDHIRMTGSFP*