AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044379


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044379 MtrunA17_Chr8g0373841 87.347 490 52 4 1 480 1 490 0.0 871
MsaG044379 MtrunA17_Chr4g0075321 59.236 314 103 3 39 345 384 679 2.48e-114 362
MsaG044379 MtrunA17_Chr5g0403241 58.271 266 100 5 218 475 817 1079 4.07e-91 303
MsaG044379 MtrunA17_Chr5g0403241 41.096 146 79 1 56 194 507 652 2.81e-26 113
MsaG044379 MtrunA17_Chr8g0373821 59.756 246 51 2 20 228 6 240 2.20e-87 278
MsaG044379 MtrunA17_Chr4g0075341 34.586 266 69 5 58 322 205 366 3.22e-35 135
MsaG044379 MtrunA17_Chr4g0075341 56.897 58 25 0 58 115 55 112 3.62e-14 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044379 AT2G23740.1 50.254 197 96 2 252 448 722 916 4.14e-55 199
MsaG044379 AT2G23740.1 41.353 133 70 2 45 169 404 536 5.67e-21 97.4
MsaG044379 AT2G23740.3 50.254 197 96 2 252 448 729 923 4.40e-55 199
MsaG044379 AT2G23740.3 41.353 133 70 2 45 169 411 543 6.32e-21 97.4
MsaG044379 AT2G23740.6 50.254 197 96 2 252 448 729 923 4.40e-55 199
MsaG044379 AT2G23740.6 41.353 133 70 2 45 169 411 543 6.32e-21 97.4
MsaG044379 AT2G23740.2 50.254 197 96 2 252 448 729 923 4.40e-55 199
MsaG044379 AT2G23740.2 41.353 133 70 2 45 169 411 543 6.32e-21 97.4
MsaG044379 AT2G23740.4 50.254 197 96 2 252 448 729 923 4.86e-55 199
MsaG044379 AT2G23740.4 41.353 133 70 2 45 169 411 543 5.63e-21 97.4
MsaG044379 AT2G23740.5 50.254 197 96 2 252 448 729 923 4.86e-55 199
MsaG044379 AT2G23740.5 41.353 133 70 2 45 169 411 543 5.63e-21 97.4

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTCAGATAGAGCCTTTC+TGG 0.146763 8:+59325054 None:intergenic
TCCAAACCTCTTTCAAATTT+AGG 0.154205 8:+59325241 None:intergenic
GGCGAAACGCTCAAGCTTTC+CGG 0.154625 8:-59325919 MsaT044379.1:CDS
ATTCCTTGTGGTTGTCATTT+TGG 0.199979 8:-59325607 MsaT044379.1:CDS
TTGATCTCCAATGGACTTTC+CGG 0.248032 8:+59325994 None:intergenic
CCAGCCTCATGCAGATAAAT+TGG 0.273277 8:-59325125 MsaT044379.1:CDS
TTGGAAATATAGAGGAATTA+TGG 0.274132 8:-59325588 MsaT044379.1:CDS
GGCAAATAAGTTGCTTGAAA+TGG 0.279485 8:-59325161 MsaT044379.1:CDS
CCTCTTTCAAATTTAGGATT+AGG 0.281407 8:+59325247 None:intergenic
TTCTTCGCAAAGAACATCTT+TGG 0.285228 8:-59325940 MsaT044379.1:CDS
ACGCAAATATGTTCTTCATT+AGG 0.299340 8:+59326435 None:intergenic
CTTGACATTGTCGACCCTTT+TGG 0.303194 8:+59326328 None:intergenic
TCCTAAATTTGAAAGAGGTT+TGG 0.365758 8:-59325242 MsaT044379.1:CDS
TGTACGAGATATTGAATTTG+AGG 0.372830 8:+59326089 None:intergenic
CCATCATCAAGCTGCTCATA+TGG 0.373746 8:-59325353 MsaT044379.1:CDS
GCCTGTCCACTGATCATTAC+CGG 0.388651 8:+59325900 None:intergenic
GCAGTTGTACCTCCACAAAA+TGG 0.390067 8:+59326195 None:intergenic
TCCAATGGTTGCCAAGTGTT+TGG 0.392168 8:+59325782 None:intergenic
GCAAATAAGTTGCTTGAAAT+GGG 0.399729 8:-59325160 MsaT044379.1:CDS
AAGAGACACTATGGGCAGTT+TGG 0.400556 8:-59325655 MsaT044379.1:CDS
AGTCTTTGCAAATCAGGCTC+TGG 0.401375 8:+59326031 None:intergenic
CGATCTAGCCAGCAGGCGCT+TGG 0.413605 8:-59325326 MsaT044379.1:CDS
ACACCAAGTCTTTGCAAATC+AGG 0.417723 8:+59326025 None:intergenic
GGAAGAATCTCAATGCTTAG+AGG 0.420258 8:-59325104 MsaT044379.1:CDS
AGTTATCGAAAGCTTAAATT+CGG 0.424504 8:+59325540 None:intergenic
CCTAATCCTAAATTTGAAAG+AGG 0.427935 8:-59325247 MsaT044379.1:CDS
GTTTGGAAGAAGCATCAAAT+AGG 0.428853 8:-59325225 MsaT044379.1:CDS
AGACTTGGTGTTTGTACATC+CGG 0.429428 8:-59326013 MsaT044379.1:CDS
ATCGAAAGCTTAAATTCGGC+AGG 0.430592 8:+59325544 None:intergenic
TTTCATGACTTTCCATCCAA+TGG 0.430775 8:+59325767 None:intergenic
CCAATTTATCTGCATGAGGC+TGG 0.431124 8:+59325125 None:intergenic
ACTCTTTGCCAAGCGCCTGC+TGG 0.443750 8:+59325318 None:intergenic
CAGTGACAGTTCATCTGTAA+TGG 0.451819 8:-59326370 MsaT044379.1:CDS
GTTCGCGGAAGTTTAGTTGC+AGG 0.454226 8:-59325417 MsaT044379.1:CDS
TAATTTCAAGAATAGAAATC+AGG 0.462085 8:-59325182 MsaT044379.1:CDS
TTCTCCTAAAATGATTGAGC+AGG 0.465200 8:-59325485 MsaT044379.1:CDS
TGATGATGGCGACCGAGATC+AGG 0.469915 8:+59325367 None:intergenic
GTCTTTGCAAATCAGGCTCT+GGG 0.470035 8:+59326032 None:intergenic
ACCAAACACTTGGCAACCAT+TGG 0.477256 8:-59325783 MsaT044379.1:CDS
GACTCTTATAGAAAACCAAA+AGG 0.481717 8:-59326343 MsaT044379.1:CDS
CCATATGAGCAGCTTGATGA+TGG 0.488631 8:+59325353 None:intergenic
TGAGAGTGCTGTTAAGTGTA+AGG 0.490329 8:-59325830 MsaT044379.1:CDS
TTGGATGGAAAGTCATGAAA+AGG 0.491304 8:-59325764 MsaT044379.1:CDS
TCATCATTGACGATTGACTC+TGG 0.492547 8:+59325970 None:intergenic
AGTGAAGGAATCAAAACAAA+AGG 0.493161 8:+59325708 None:intergenic
CATGTTCAGAAGAGACACTA+TGG 0.495523 8:-59325664 MsaT044379.1:CDS
ACATCTGCACAAAGCTGAGA+TGG 0.500567 8:-59326118 MsaT044379.1:CDS
GAGATATTGAATTTGAGGTC+TGG 0.501478 8:+59326094 None:intergenic
CAGTGCGTGAGAACCGCAAT+TGG 0.508352 8:-59326282 MsaT044379.1:CDS
GTTTCGTTTATCTACCAGAA+AGG 0.508712 8:-59325068 MsaT044379.1:CDS
TATGCTCAGAGACTAAAATC+TGG 0.515386 8:-59325283 MsaT044379.1:CDS
GAGCCTGATTTGCAAAGACT+TGG 0.521361 8:-59326028 MsaT044379.1:CDS
TTCAAATTTAGGATTAGGAC+GGG 0.522702 8:+59325252 None:intergenic
ATTAGCTTTAGCCCTTCTCC+TGG 0.523241 8:+59325201 None:intergenic
TCCGGTAATGATCAGTGGAC+AGG 0.523358 8:-59325901 MsaT044379.1:CDS
TGGAGCGCGATCTAGCCAGC+AGG 0.523628 8:-59325333 MsaT044379.1:CDS
AGCAGGCGCTTGGCAAAGAG+TGG 0.523735 8:-59325316 MsaT044379.1:CDS
ACAAATCAAGGAGCAGCTTA+CGG 0.524363 8:-59326459 MsaT044379.1:CDS
CAATATCTCGTACATTAAAG+AGG 0.524991 8:-59326080 MsaT044379.1:CDS
GACTCTGGATTGATCTCCAA+TGG 0.529884 8:+59325985 None:intergenic
CTGAGAATCGAAGTGGTTCG+CGG 0.530378 8:-59325432 MsaT044379.1:CDS
GTGTATATTGTTACCAATTG+CGG 0.530995 8:+59326269 None:intergenic
TGTACATCCGGAAAGTCCAT+TGG 0.533385 8:-59326001 MsaT044379.1:CDS
CATCAAATAGGACCAGGAGA+AGG 0.534319 8:-59325213 MsaT044379.1:CDS
TTTGATTTACTACCTGATCT+CGG 0.536142 8:-59325379 MsaT044379.1:CDS
AACACTTGGCAACCATTGGA+TGG 0.536290 8:-59325779 MsaT044379.1:CDS
TTTCAAATTTAGGATTAGGA+CGG 0.543514 8:+59325251 None:intergenic
ATCAAATAGGACCAGGAGAA+GGG 0.546017 8:-59325212 MsaT044379.1:CDS
TTTATCTGCATGAGGCTGGA+TGG 0.546716 8:+59325129 None:intergenic
TCTTCCAATTTATCTGCATG+AGG 0.563398 8:+59325121 None:intergenic
GATTGCTCTGAGACAAATCA+AGG 0.567116 8:-59326471 MsaT044379.1:CDS
GACAGTTCATCTGTAATGGA+AGG 0.570153 8:-59326366 MsaT044379.1:CDS
ACCAGAAAGGCTCTATCTGA+AGG 0.579442 8:-59325055 MsaT044379.1:CDS
CATCTGCACAAAGCTGAGAT+GGG 0.579947 8:-59326117 MsaT044379.1:CDS
ACTCTTATAGAAAACCAAAA+GGG 0.580021 8:-59326342 MsaT044379.1:CDS
TGGACAGGCTCTCCTAGCTA+TGG 0.583120 8:-59325886 MsaT044379.1:CDS
TTATGATCATTACCATAGCT+AGG 0.583706 8:+59325874 None:intergenic
TGTGGAGGTACAACTGCTGC+TGG 0.586893 8:-59326189 MsaT044379.1:CDS
TCTCCTAAAATGATTGAGCA+GGG 0.608885 8:-59325484 MsaT044379.1:CDS
GAAGCACAGTGGTTGTTCAG+AGG 0.608970 8:-59325742 MsaT044379.1:CDS
GATAATTCTGAGAATCGAAG+TGG 0.614192 8:-59325439 MsaT044379.1:CDS
AGCTTTCCGGTAATGATCAG+TGG 0.616263 8:-59325906 MsaT044379.1:CDS
CATATATCGAAGACAAGGGA+AGG 0.617551 8:-59326305 MsaT044379.1:CDS
GAGCAACTAACATTGTCTAC+CGG 0.626990 8:-59325514 MsaT044379.1:CDS
TCAAGCATATATCGAAGACA+AGG 0.630094 8:-59326310 MsaT044379.1:CDS
ACAGTTCATCTGTAATGGAA+GGG 0.632090 8:-59326365 MsaT044379.1:CDS
AAGAAGCATCAAATAGGACC+AGG 0.634670 8:-59325219 MsaT044379.1:CDS
ATGTTCAGAAGAGACACTAT+GGG 0.654163 8:-59325663 MsaT044379.1:CDS
TCGAAAGCTTAAATTCGGCA+GGG 0.657128 8:+59325545 None:intergenic
AAAGCTGAACAAAATTGCAG+TGG 0.668916 8:-59326057 MsaT044379.1:CDS
CAAGCATATATCGAAGACAA+GGG 0.692685 8:-59326309 MsaT044379.1:CDS
TTTCCAAAATGACAACCACA+AGG 0.715599 8:+59325604 None:intergenic
GTCATGAAAAGGAAGCACAG+TGG 0.767507 8:-59325753 MsaT044379.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 59325036 59326511 59325036 ID=MsaG044379
Chr8 mRNA 59325036 59326511 59325036 ID=MsaT044379.1;Parent=MsaG044379
Chr8 exon 59325036 59326511 59325036 ID=MsaT044379.1.exon1;Parent=MsaT044379.1
Chr8 CDS 59325036 59326511 59325036 ID=cds.MsaT044379.1;Parent=MsaT044379.1
Gene Sequence

>MsaT044379.1

ATGGAAACTCTTTATACTGATTGCTCTGAGACAAATCAAGGAGCAGCTTACGGACCTAATGAAGAACATATTTGCGTTGAAAATCGCGAATATGATATTGCAGAACTTGATGATGAACTCAGTGACAGTTCATCTGTAATGGAAGGGACTCTTATAGAAAACCAAAAGGGTCGACAATGTCAAGCATATATCGAAGACAAGGGAAGGCAGTGCGTGAGAACCGCAATTGGTAACAATATACACTGTTGTGTGCATTTCTTAAGTAAAAAAGAAAGGCGTGTTAAAGCAATCACTCCATTTTGTGGAGGTACAACTGCTGCTGGTACTAGATGTAAGAATCGTTCCCTTCCCAGATTATCTTTTTGTAAGAAACATCTGCACAAAGCTGAGATGGGCCAGACCTCAAATTCAATATCTCGTACATTAAAGAGGAAAGCTGAACAAAATTGCAGTGGTTCCCAGAGCCTGATTTGCAAAGACTTGGTGTTTGTACATCCGGAAAGTCCATTGGAGATCAATCCAGAGTCAATCGTCAATGATGATGATTCTTTCTTCGCAAAGAACATCTTTGGCGAAACGCTCAAGCTTTCCGGTAATGATCAGTGGACAGGCTCTCCTAGCTATGGTAATGATCATAACGAAGACTCTTGTATTGATAATGAGAGTGCTGTTAAGTGTAAGGTCTGCTTTGAAGAATTTTCTGATAACCAAACACTTGGCAACCATTGGATGGAAAGTCATGAAAAGGAAGCACAGTGGTTGTTCAGAGGTTATGCATGTGCCTTTTGTTTTGATTCCTTCACTAACAAAAAACTATTGGAATCACATGTTCAGAAGAGACACTATGGGCAGTTTGGTGAACACTGCTTGCTTTTTCTATGTATTCCTTGTGGTTGTCATTTTGGAAATATAGAGGAATTATGGTTGCATGTTAAGTCAGTTCACCCTGCCGAATTTAAGCTTTCGATAACTCATGAGCAACTAACATTGTCTACCGGTGATGATTCTCCTAAAATGATTGAGCAGGGAAATGAAGCTTCTTTGTACAATGATAATTCTGAGAATCGAAGTGGTTCGCGGAAGTTTAGTTGCAGGTTTTGTGATTTGAAATTTGATTTACTACCTGATCTCGGTCGCCATCATCAAGCTGCTCATATGGAGCGCGATCTAGCCAGCAGGCGCTTGGCAAAGAGTGGAGTTTGTTATTATGCTCAGAGACTAAAATCTGGCAGACTTACCCGTCCTAATCCTAAATTTGAAAGAGGTTTGGAAGAAGCATCAAATAGGACCAGGAGAAGGGCTAAAGCTAATTTCAAGAATAGAAATCAGGCAAATAAGTTGCTTGAAATGGGAGAAATATCCATCCAGCCTCATGCAGATAAATTGGAAGAATCTCAATGCTTAGAGGCTGCAAATGTTTTGTTTCGTTTATCTACCAGAAAGGCTCTATCTGAAGGCAGCAAAATGTTGCAGTGA

Protein sequence

>MsaT044379.1

METLYTDCSETNQGAAYGPNEEHICVENREYDIAELDDELSDSSSVMEGTLIENQKGRQCQAYIEDKGRQCVRTAIGNNIHCCVHFLSKKERRVKAITPFCGGTTAAGTRCKNRSLPRLSFCKKHLHKAEMGQTSNSISRTLKRKAEQNCSGSQSLICKDLVFVHPESPLEINPESIVNDDDSFFAKNIFGETLKLSGNDQWTGSPSYGNDHNEDSCIDNESAVKCKVCFEEFSDNQTLGNHWMESHEKEAQWLFRGYACAFCFDSFTNKKLLESHVQKRHYGQFGEHCLLFLCIPCGCHFGNIEELWLHVKSVHPAEFKLSITHEQLTLSTGDDSPKMIEQGNEASLYNDNSENRSGSRKFSCRFCDLKFDLLPDLGRHHQAAHMERDLASRRLAKSGVCYYAQRLKSGRLTRPNPKFERGLEEASNRTRRRAKANFKNRNQANKLLEMGEISIQPHADKLEESQCLEAANVLFRLSTRKALSEGSKMLQ*