AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044397

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044397 MtrunA17_Chr8g0374111 86.777 242 19 5 1 242 1 229 2.58e-122 347
MsaG044397 MtrunA17_Chr8g0341671 74.286 70 17 1 17 85 7 76 4.36e-32 117
MsaG044397 MtrunA17_Chr4g0031411 54.717 106 35 3 17 109 54 159 7.90e-31 116
MsaG044397 MtrunA17_Chr2g0328021 64.000 75 27 0 22 96 55 129 8.14e-31 115
MsaG044397 MtrunA17_Chr8g0346551 41.844 141 80 1 23 163 18 156 1.77e-30 114
MsaG044397 MtrunA17_Chr2g0326001 42.515 167 89 3 12 174 24 187 4.13e-30 113
MsaG044397 MtrunA17_Chr4g0047001 61.842 76 29 0 31 106 57 132 4.80e-30 115
MsaG044397 MtrunA17_Chr4g0010151 41.304 184 85 6 17 190 61 231 1.56e-29 113
MsaG044397 MtrunA17_Chr4g0030051 61.728 81 23 1 1 81 21 93 3.87e-29 110
MsaG044397 MtrunA17_Chr8g0374251 58.228 79 33 0 26 104 65 143 4.17e-29 112
MsaG044397 MtrunA17_Chr7g0237921 44.156 154 53 4 17 142 55 203 6.90e-29 111
MsaG044397 MtrunA17_Chr7g0223311 73.770 61 16 0 31 91 79 139 2.92e-28 110
MsaG044397 MtrunA17_Chr1g0188851 62.857 70 26 0 14 83 28 97 4.24e-28 108
MsaG044397 MtrunA17_Chr3g0127201 70.588 68 18 2 18 85 17 82 7.31e-28 107
MsaG044397 MtrunA17_Chr3g0092111 34.737 190 103 4 29 211 136 311 2.56e-27 108
MsaG044397 MtrunA17_Chr4g0047761 78.947 57 11 1 27 83 70 125 7.12e-27 105
MsaG044397 MtrunA17_Chr5g0411951 71.429 56 16 0 24 79 38 93 9.97e-27 105
MsaG044397 MtrunA17_Chr8g0367161 54.321 81 34 1 29 109 32 109 1.13e-26 105
MsaG044397 MtrunA17_Chr2g0282051 75.439 57 13 1 27 83 44 99 2.36e-26 103
MsaG044397 MtrunA17_Chr2g0282511 83.673 49 8 0 29 77 45 93 4.75e-26 103
MsaG044397 MtrunA17_Chr4g0061761 79.245 53 11 0 27 79 38 90 4.97e-26 103
MsaG044397 MtrunA17_Chr3g0117601 70.312 64 15 1 16 79 52 111 5.22e-26 103
MsaG044397 MtrunA17_Chr2g0282441 61.972 71 23 1 10 80 10 76 6.75e-26 103
MsaG044397 MtrunA17_Chr7g0217581 38.065 155 71 4 29 178 232 366 1.69e-25 104
MsaG044397 MtrunA17_Chr3g0126201 47.748 111 45 2 2 112 34 131 3.68e-25 101
MsaG044397 MtrunA17_Chr2g0308011 81.633 49 9 0 29 77 37 85 3.90e-25 98.6
MsaG044397 MtrunA17_Chr4g0042481 63.934 61 22 0 29 89 122 182 4.01e-25 102
MsaG044397 MtrunA17_Chr6g0453321 37.662 154 76 4 29 176 136 275 4.19e-25 102
MsaG044397 MtrunA17_Chr2g0283811 67.273 55 18 0 26 80 44 98 1.19e-24 95.9
MsaG044397 MtrunA17_Chr8g0355261 62.500 64 23 1 29 92 118 180 2.66e-24 100
MsaG044397 MtrunA17_Chr5g0417571 63.934 61 22 0 21 81 130 190 3.00e-24 98.6
MsaG044397 MtrunA17_Chr1g0177251 51.163 86 36 2 31 110 14 99 3.25e-24 97.8
MsaG044397 MtrunA17_Chr7g0254161 39.655 116 63 2 31 139 103 218 4.18e-24 99.8
MsaG044397 MtrunA17_Chr6g0460731 39.695 131 73 3 29 156 96 223 9.50e-24 97.8
MsaG044397 MtrunA17_Chr5g0417551 73.585 53 14 0 29 81 127 179 3.29e-23 96.7
MsaG044397 MtrunA17_Chr5g0417561 73.585 53 14 0 29 81 119 171 3.96e-23 96.3
MsaG044397 MtrunA17_Chr6g0460721 56.716 67 28 1 29 94 89 155 7.30e-23 95.1
MsaG044397 MtrunA17_Chr5g0417601 62.295 61 23 0 21 81 132 192 1.47e-22 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044397 AT3G50410.1 48.289 263 105 9 1 242 1 253 1.70e-58 186
MsaG044397 AT5G66940.1 46.186 236 94 9 4 223 3 221 8.42e-50 163
MsaG044397 AT1G51700.1 47.561 164 53 6 24 171 28 174 2.04e-35 125
MsaG044397 AT3G21270.1 43.293 164 76 5 29 184 29 183 1.04e-32 118
MsaG044397 AT5G60850.1 66.265 83 25 2 27 109 49 128 1.01e-31 118
MsaG044397 AT5G60200.1 63.636 88 24 2 22 109 48 127 7.66e-31 115
MsaG044397 AT3G45610.1 52.294 109 43 2 2 110 13 112 1.03e-30 114
MsaG044397 AT1G47655.1 39.496 238 101 9 11 242 9 209 1.50e-30 113
MsaG044397 AT3G55370.3 63.514 74 25 2 21 93 114 186 2.18e-28 110
MsaG044397 AT1G07640.1 74.138 58 15 0 29 86 21 78 2.19e-28 108
MsaG044397 AT3G55370.1 63.514 74 25 2 21 93 69 141 3.02e-28 109
MsaG044397 AT5G65590.1 72.131 61 17 0 23 83 35 95 3.09e-28 109
MsaG044397 AT3G55370.2 63.514 74 25 2 21 93 69 141 3.50e-28 110
MsaG044397 AT1G07640.2 74.138 58 15 0 29 86 77 134 4.08e-28 109
MsaG044397 AT1G07640.3 74.138 58 15 0 29 86 85 142 5.25e-28 109
MsaG044397 AT1G28310.1 75.862 58 14 0 29 86 27 84 6.44e-28 108
MsaG044397 AT5G62430.1 63.158 76 27 1 29 103 54 129 8.57e-28 108
MsaG044397 AT1G28310.2 75.862 58 14 0 29 86 51 108 8.88e-28 108
MsaG044397 AT4G38000.1 44.615 130 66 3 10 133 19 148 1.90e-27 105
MsaG044397 AT5G39660.3 56.098 82 34 1 29 108 138 219 2.94e-27 108
MsaG044397 AT5G39660.1 56.098 82 34 1 29 108 138 219 2.94e-27 108
MsaG044397 AT5G39660.2 56.098 82 34 1 29 108 138 219 2.94e-27 108
MsaG044397 AT3G61850.2 70.769 65 18 1 23 87 56 119 3.02e-27 106
MsaG044397 AT3G61850.3 70.769 65 18 1 23 87 56 119 3.02e-27 106
MsaG044397 AT4G24060.1 52.083 96 43 2 18 111 43 137 3.91e-27 107
MsaG044397 AT5G02460.1 63.636 77 17 2 17 83 74 149 4.01e-27 107
MsaG044397 AT3G47500.1 40.741 135 64 3 1 121 84 216 4.06e-27 108
MsaG044397 AT3G61850.1 70.769 65 18 1 23 87 68 131 4.11e-27 106
MsaG044397 AT3G61850.5 70.769 65 18 1 23 87 56 119 5.34e-27 106
MsaG044397 AT3G61850.4 70.769 65 18 1 23 87 68 131 5.94e-27 106
MsaG044397 AT2G28810.2 73.684 57 15 0 24 80 72 128 8.31e-27 105
MsaG044397 AT2G28810.1 73.684 57 15 0 24 80 89 145 1.13e-26 105
MsaG044397 AT2G28510.1 38.922 167 66 4 31 162 50 215 2.85e-26 103
MsaG044397 AT2G37590.1 59.722 72 29 0 12 83 72 143 4.45e-26 103
MsaG044397 AT4G21050.1 64.179 67 23 1 31 97 26 91 1.56e-25 100
MsaG044397 AT1G21340.1 64.706 68 21 1 25 89 31 98 2.08e-25 100
MsaG044397 AT4G00940.1 70.690 58 17 0 26 83 65 122 4.24e-25 100
MsaG044397 AT4G00940.3 70.690 58 17 0 26 83 65 122 4.24e-25 100
MsaG044397 AT4G00940.2 70.690 58 17 0 26 83 65 122 4.24e-25 100
MsaG044397 AT3G52440.1 63.636 66 24 0 18 83 14 79 1.16e-24 98.6
MsaG044397 AT5G62940.1 67.188 64 17 1 16 79 64 123 1.16e-24 100
MsaG044397 AT1G26790.1 60.000 70 27 1 29 97 111 180 1.35e-24 100
MsaG044397 AT3G52440.2 63.636 66 24 0 18 83 33 98 1.53e-24 98.6
MsaG044397 AT2G46590.1 74.074 54 14 0 23 76 62 115 1.68e-24 100
MsaG044397 AT2G46590.2 74.074 54 14 0 23 76 74 127 1.96e-24 100
MsaG044397 AT1G29160.1 45.000 100 55 0 29 128 62 161 2.93e-24 95.5
MsaG044397 AT4G21040.1 62.687 67 23 1 31 97 27 91 6.19e-24 96.3
MsaG044397 AT1G69570.1 69.811 53 16 0 29 81 132 184 6.21e-24 99.4
MsaG044397 AT4G21080.1 64.179 67 22 1 31 97 27 91 2.05e-23 95.5
MsaG044397 AT1G64620.1 60.811 74 20 2 14 78 25 98 3.71e-23 96.7
MsaG044397 AT2G34140.1 67.925 53 17 0 29 81 58 110 6.69e-23 92.0
MsaG044397 AT4G21030.1 60.000 50 20 0 31 80 23 72 1.91e-13 67.4

Find 89 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCAAAACTCTGGGTTTC+TGG 0.121250 8:-59612237 MsaT044397.1:CDS
TTCTGGCATTAGGTGGATTT+GGG 0.223026 8:-59612220 MsaT044397.1:CDS
CCGTCAATACCGCCGGAAAA+TGG 0.237022 8:+59612320 None:intergenic
GGTGTTCCTGTTGTGTCTTC+TGG 0.239637 8:-59612107 MsaT044397.1:CDS
TTTCTGGCATTAGGTGGATT+TGG 0.249923 8:-59612221 MsaT044397.1:CDS
CATTAGGTGGATTTGGGCTT+GGG 0.256673 8:-59612214 MsaT044397.1:CDS
GCTATTTCAACACCAGTAAA+TGG 0.284985 8:-59612002 MsaT044397.1:CDS
TCCTCCTTGGACGGCGGAGA+TGG 0.289163 8:+59612352 None:intergenic
GCATTAGGTGGATTTGGGCT+TGG 0.292718 8:-59612215 MsaT044397.1:CDS
GGTGGATTTGGGCTTGGGCT+TGG 0.292816 8:-59612209 MsaT044397.1:CDS
TTTGGGCTTGGGCTTGGACA+TGG 0.300293 8:-59612203 MsaT044397.1:CDS
GCAAAGCTTGTCGCCGCTAC+TGG 0.340843 8:-59612532 MsaT044397.1:CDS
TTGGGCTTGGGCTTGGACAT+GGG 0.342240 8:-59612202 MsaT044397.1:CDS
TTTGATCCATCCACCATTCC+TGG 0.347481 8:+59612134 None:intergenic
GGGCTTGAAGAAATGGGCTT+TGG 0.347490 8:-59612182 MsaT044397.1:CDS
GTTGTGTCTTCTGGTGTTGT+TGG 0.348865 8:-59612098 MsaT044397.1:CDS
AAGAAATGGGCTTTGGGATT+GGG 0.351153 8:-59612175 MsaT044397.1:CDS
TGCTCCGGTGCCGGTGCACC+GGG 0.353622 8:+59612644 None:intergenic
TGGACATGGGCTTGAAGAAA+TGG 0.357925 8:-59612189 MsaT044397.1:CDS
TGGGATTGGGAGAACAGGTT+GGG 0.361604 8:-59612162 MsaT044397.1:CDS
GGTGATTGTTTCTCTTGGCC+TGG 0.369523 8:-59612029 MsaT044397.1:CDS
GGCTTTGGGATTGGGAGAAC+AGG 0.373009 8:-59612167 MsaT044397.1:CDS
TTGCTCCGGTGCCGGTGCAC+CGG 0.375742 8:+59612643 None:intergenic
GGCTTGAAGAAATGGGCTTT+GGG 0.379257 8:-59612181 MsaT044397.1:CDS
GTTCATTTGAGACCATTTAC+TGG 0.380286 8:+59611990 None:intergenic
GAAGAAATGGGCTTTGGGAT+TGG 0.382875 8:-59612176 MsaT044397.1:CDS
GTGGTGGTGATTGTTTCTCT+TGG 0.384765 8:-59612034 MsaT044397.1:CDS
CCTCCTTGGACGGCGGAGAT+GGG 0.390226 8:+59612353 None:intergenic
TGTTGATCGGCGGCTTTCAC+CGG 0.408674 8:+59612682 None:intergenic
GGTGCACCGGGGCGGATTTG+AGG 0.417132 8:+59612656 None:intergenic
AACTCTGGGTTTCTGGCATT+AGG 0.420912 8:-59612230 MsaT044397.1:CDS
CCGCCCCGGTGCACCGGCAC+CGG 0.422541 8:-59612648 MsaT044397.1:CDS
TGCCGTGACTTCAGCGGCGC+CGG 0.425658 8:-59612402 MsaT044397.1:CDS
AACAACAACAATCAAAACTC+TGG 0.431082 8:-59612245 MsaT044397.1:CDS
AGATTCTCTTGCTCCGGTGC+CGG 0.436640 8:+59612635 None:intergenic
ATCTCCGCCGTCCAAGGAGG+AGG 0.437854 8:-59612350 MsaT044397.1:CDS
GGACATGGGCTTGAAGAAAT+GGG 0.438047 8:-59612188 MsaT044397.1:CDS
TTGGGATTGGGAGAACAGGT+TGG 0.446014 8:-59612163 MsaT044397.1:CDS
ATGCTGAGTACGAGAGCGTT+TGG 0.449036 8:+59612454 None:intergenic
ACAACACCAGAAGACACAAC+AGG 0.458334 8:+59612101 None:intergenic
CCGGCGGTATTGACGGTGAT+GGG 0.466545 8:-59612313 MsaT044397.1:CDS
GGAAACACGTGGCAGTTAGA+AGG 0.471025 8:-59612077 MsaT044397.1:CDS
ATGGAAAAGACCCTCCTCCT+TGG 0.474800 8:+59612339 None:intergenic
CTCCGGCGCCGCTGAAGTCA+CGG 0.478425 8:+59612400 None:intergenic
TTTCTGGTACCACCGCCGAC+GGG 0.484650 8:+59612482 None:intergenic
CGTGACATCCCCGTCGGCGG+TGG 0.490474 8:-59612491 MsaT044397.1:CDS
CATGGGAGATTCTCTTGCTC+CGG 0.492080 8:+59612629 None:intergenic
CAGTTAGAAGGTGGTGAAGG+TGG 0.495978 8:-59612065 MsaT044397.1:CDS
TCTCTAAACAATGACATCTT+CGG 0.498140 8:-59612708 None:intergenic
TCAAATCCGCCCCGGTGCAC+CGG 0.515212 8:-59612654 MsaT044397.1:CDS
TCCGGCGGTATTGACGGTGA+TGG 0.517628 8:-59612314 MsaT044397.1:CDS
CGCCGCTGAAGTCACGGCAG+AGG 0.520050 8:+59612406 None:intergenic
AAGCAGAATATGAGTGTTTG+TGG 0.522514 8:-59612290 MsaT044397.1:CDS
GCTTGATAGTGGTGGTGCTC+CGG 0.532605 8:+59612383 None:intergenic
GTTGAATCACAACGTGGACA+TGG 0.538502 8:+59612611 None:intergenic
TCTGGGTTTCTGGCATTAGG+TGG 0.547157 8:-59612227 MsaT044397.1:CDS
CACCGCCGACGGGGATGTCA+CGG 0.552285 8:+59612492 None:intergenic
CACGGCAGAGGAAGAAGATG+AGG 0.555442 8:+59612418 None:intergenic
GTAGTAACAAAACTTAGTGT+TGG 0.560310 8:+59612589 None:intergenic
ACACCCATCTCCGCCGTCCA+AGG 0.561242 8:-59612356 MsaT044397.1:CDS
TTCTGGTACCACCGCCGACG+GGG 0.573655 8:+59612483 None:intergenic
ACAACAACAATCAAAACTCT+GGG 0.575835 8:-59612244 MsaT044397.1:CDS
CCCATCACCGTCAATACCGC+CGG 0.580375 8:+59612313 None:intergenic
TCTCCGCCGTCCAAGGAGGA+GGG 0.582119 8:-59612349 MsaT044397.1:CDS
ACAATGACATCTTCGGACTC+CGG 0.583202 8:-59612701 MsaT044397.1:CDS
GGTGTTGAAATAGCAAGTCC+AGG 0.592777 8:+59612011 None:intergenic
TGGGTGTCATTGCTTGATAG+TGG 0.598785 8:+59612372 None:intergenic
CCGGTGCCGGTGCACCGGGG+CGG 0.610074 8:+59612648 None:intergenic
AGACCCTCCTCCTTGGACGG+CGG 0.612466 8:+59612346 None:intergenic
CTCCGTGACATCCCCGTCGG+CGG 0.612747 8:-59612494 MsaT044397.1:CDS
TTCCTCTGCCGTGACTTCAG+CGG 0.618657 8:-59612408 MsaT044397.1:CDS
TGTCGCCGCTACTGGACTCA+CGG 0.619346 8:-59612524 MsaT044397.1:CDS
ACAAAACCTCAAATCCGCCC+CGG 0.621584 8:-59612662 MsaT044397.1:CDS
TGGCAGTTAGAAGGTGGTGA+AGG 0.635231 8:-59612068 MsaT044397.1:CDS
CAAGCTTTGCAGAAATGACG+TGG 0.636873 8:+59612545 None:intergenic
ACCCTCCGTGACATCCCCGT+CGG 0.638321 8:-59612497 MsaT044397.1:CDS
AAAAGACCCTCCTCCTTGGA+CGG 0.641146 8:+59612343 None:intergenic
TTGAATCACAACGTGGACAT+GGG 0.644805 8:+59612612 None:intergenic
GTGTCATTGCTTGATAGTGG+TGG 0.649877 8:+59612375 None:intergenic
CCCATCTCCGCCGTCCAAGG+AGG 0.650434 8:-59612353 MsaT044397.1:CDS
TACCACCGTGAGTCCAGTAG+CGG 0.656577 8:+59612519 None:intergenic
GCTCCGGTGCCGGTGCACCG+GGG 0.657998 8:+59612645 None:intergenic
GCCGACGGGGATGTCACGGA+GGG 0.667751 8:+59612496 None:intergenic
AACACGTGGCAGTTAGAAGG+TGG 0.673819 8:-59612074 MsaT044397.1:CDS
CGCCGACGGGGATGTCACGG+AGG 0.674019 8:+59612495 None:intergenic
ATGGTGGATGGATCAAACAT+TGG 0.699118 8:-59612128 MsaT044397.1:CDS
CTGGTGTTGTTGGAAACACG+TGG 0.703407 8:-59612088 MsaT044397.1:CDS
CGCCGCTACTGGACTCACGG+TGG 0.745530 8:-59612521 MsaT044397.1:CDS
GTGTTGGTTGAATCACAACG+TGG 0.795975 8:+59612605 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 59611992 59612720 59611992 ID=MsaG044397
Chr8 mRNA 59611992 59612720 59611992 ID=MsaT044397.1;Parent=MsaG044397
Chr8 exon 59611992 59612720 59611992 ID=MsaT044397.1.exon1;Parent=MsaT044397.1
Chr8 CDS 59611992 59612720 59611992 ID=cds.MsaT044397.1;Parent=MsaT044397.1
Gene Sequence

>MsaT044397.1

ATGACATCTTCGGACTCCGGTGAAAGCCGCCGATCAACAAAACCTCAAATCCGCCCCGGTGCACCGGCACCGGAGCAAGAGAATCTCCCATGTCCACGTTGTGATTCAACCAACACTAAGTTTTGTTACTACAACAACTACAACTACTCTCAGCCACGTCATTTCTGCAAAGCTTGTCGCCGCTACTGGACTCACGGTGGTACCCTCCGTGACATCCCCGTCGGCGGTGGTACCAGAAAAAACGCCAAACGCTCTCGTACTCAGCATGTTGCTGTCACTTCCTCATCTTCTTCCTCTGCCGTGACTTCAGCGGCGCCGGAGCACCACCACTATCAAGCAATGACACCCATCTCCGCCGTCCAAGGAGGAGGGTCTTTTCCATTTTCCGGCGGTATTGACGGTGATGGGAAGCAGAATATGAGTGTTTGTGGAAGCTTCACTTCATTGCTGAACAACAACAACAATCAAAACTCTGGGTTTCTGGCATTAGGTGGATTTGGGCTTGGGCTTGGACATGGGCTTGAAGAAATGGGCTTTGGGATTGGGAGAACAGGTTGGGCTTTTCCAGGAATGGTGGATGGATCAAACATTGGTGTTCCTGTTGTGTCTTCTGGTGTTGTTGGAAACACGTGGCAGTTAGAAGGTGGTGAAGGTGGTTTTGTTGGTGGTGGTGATTGTTTCTCTTGGCCTGGACTTGCTATTTCAACACCAGTAAATGGTCTCAAATGA
Protein sequence

>MsaT044397.1

MTSSDSGESRRSTKPQIRPGAPAPEQENLPCPRCDSTNTKFCYYNNYNYSQPRHFCKACRRYWTHGGTLRDIPVGGGTRKNAKRSRTQHVAVTSSSSSSAVTSAAPEHHHYQAMTPISAVQGGGSFPFSGGIDGDGKQNMSVCGSFTSLLNNNNNQNSGFLALGGFGLGLGHGLEEMGFGIGRTGWAFPGMVDGSNIGVPVVSSGVVGNTWQLEGGEGGFVGGGDCFSWPGLAISTPVNGLK*