AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044429

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044429 MtrunA17_Chr8g0374601 61.450 262 46 2 1 207 19 280 5.84e-105 304
MsaG044429 MtrunA17_Chr8g0374591 90.244 123 12 0 1 123 1 123 1.24e-79 234
MsaG044429 MtrunA17_Chr8g0374581 52.632 133 55 2 1 132 1 126 2.46e-43 145
MsaG044429 MtrunA17_Chr5g0402611 43.713 167 77 2 8 172 8 159 4.12e-39 137
MsaG044429 MtrunA17_Chr3g0097221 55.263 114 50 1 8 120 7 120 6.12e-37 130
MsaG044429 MtrunA17_Chr3g0115431 53.704 108 50 0 8 115 9 116 7.48e-37 128
MsaG044429 MtrunA17_Chr4g0058651 42.647 136 65 1 8 130 11 146 1.97e-33 119
MsaG044429 MtrunA17_Chr7g0227511 41.270 126 73 1 8 133 11 135 1.79e-32 115
MsaG044429 MtrunA17_Chr3g0117121 49.038 104 53 0 8 111 10 113 2.23e-32 116
MsaG044429 MtrunA17_Chr2g0311601 47.115 104 55 0 5 108 10 113 6.60e-31 111
MsaG044429 MtrunA17_Chr3g0106101 43.564 101 57 0 8 108 7 107 9.58e-31 111
MsaG044429 MtrunA17_Chr8g0353981 42.857 147 81 2 8 152 7 152 2.51e-30 112
MsaG044429 MtrunA17_Chr5g0437571 47.525 101 53 0 8 108 6 106 3.39e-30 109
MsaG044429 MtrunA17_Chr5g0437401 49.020 102 52 0 9 110 12 113 6.06e-30 109
MsaG044429 MtrunA17_Chr3g0114061 45.545 101 55 0 8 108 8 108 6.50e-30 108
MsaG044429 MtrunA17_Chr4g0043421 48.000 100 52 0 8 107 15 114 3.17e-29 107
MsaG044429 MtrunA17_Chr6g0449641 45.133 113 61 1 8 120 9 120 9.63e-29 105
MsaG044429 MtrunA17_Chr6g0449651 43.269 104 59 0 8 111 67 170 1.22e-28 106
MsaG044429 MtrunA17_Chr1g0200201 43.750 128 69 2 9 134 73 199 1.73e-28 107
MsaG044429 MtrunA17_Chr4g0030001 45.714 105 56 1 5 108 21 125 4.34e-28 105
MsaG044429 MtrunA17_Chr3g0129071 44.340 106 59 0 6 111 45 150 2.00e-27 104
MsaG044429 MtrunA17_Chr5g0438091 44.231 104 58 0 8 111 39 142 5.43e-27 102
MsaG044429 MtrunA17_Chr3g0113891 47.423 97 51 0 9 105 15 111 1.33e-25 98.6
MsaG044429 MtrunA17_Chr6g0460951 41.667 108 62 1 2 108 34 141 7.68e-25 97.4
MsaG044429 MtrunA17_Chr7g0225111 37.069 116 72 1 5 119 2 117 1.23e-24 94.0
MsaG044429 MtrunA17_Chr4g0055441 45.098 102 55 1 9 109 3 104 1.30e-24 95.1
MsaG044429 MtrunA17_Chr8g0351701 45.714 105 56 1 8 111 23 127 1.69e-24 96.7
MsaG044429 MtrunA17_Chr5g0432451 44.340 106 59 0 6 111 41 146 2.34e-24 96.3
MsaG044429 MtrunA17_Chr7g0260971 42.718 103 58 1 8 109 14 116 5.96e-24 95.1
MsaG044429 MtrunA17_Chr5g0444351 37.624 101 63 0 9 109 6 106 1.79e-23 91.7
MsaG044429 MtrunA17_Chr6g0460941 45.652 92 49 1 4 94 3 94 2.80e-23 89.7
MsaG044429 MtrunA17_Chr8g0342351 37.864 103 64 0 9 111 13 115 1.25e-22 92.4
MsaG044429 MtrunA17_Chr6g0452691 39.496 119 66 2 7 119 8 126 1.83e-22 90.1
MsaG044429 MtrunA17_Chr6g0452661 38.710 124 67 3 5 119 3 126 5.39e-22 90.5
MsaG044429 MtrunA17_Chr4g0059901 39.216 102 62 0 7 108 9 110 1.13e-21 90.1
MsaG044429 MtrunA17_Chr7g0245871 44.118 102 56 1 8 108 27 128 1.77e-21 89.0
MsaG044429 MtrunA17_Chr1g0184281 37.692 130 73 3 8 129 15 144 1.79e-21 88.2
MsaG044429 MtrunA17_Chr1g0184271 43.269 104 58 1 8 110 12 115 3.56e-21 88.2
MsaG044429 MtrunA17_Chr8g0351721 42.718 103 58 1 8 109 18 120 1.40e-20 85.9
MsaG044429 MtrunA17_Chr7g0261031 40.385 104 61 1 8 110 8 111 5.00e-20 84.7
MsaG044429 MtrunA17_Chr5g0439111 39.837 123 65 4 5 119 3 124 5.66e-20 84.7
MsaG044429 MtrunA17_Chr6g0452671 35.354 99 64 0 7 105 10 108 1.89e-19 82.8
MsaG044429 MtrunA17_Chr4g0040831 35.398 113 66 1 1 106 1 113 3.56e-19 82.0
MsaG044429 MtrunA17_Chr2g0330201 33.981 103 68 0 7 109 18 120 2.91e-18 81.3
MsaG044429 MtrunA17_Chr5g0435641 37.864 103 63 1 9 110 8 110 3.13e-18 79.3
MsaG044429 MtrunA17_Chr4g0046201 37.209 86 54 0 9 94 7 92 3.71e-17 77.8
MsaG044429 MtrunA17_Chr7g0245861 43.678 87 46 2 8 91 12 98 2.72e-15 70.1
MsaG044429 MtrunA17_Chr2g0326041 50.000 50 25 0 5 54 30 79 2.03e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044429 AT5G66870.1 50.806 124 59 1 8 131 7 128 2.62e-36 130
MsaG044429 AT1G65620.1 54.167 120 52 1 8 127 9 125 6.13e-34 120
MsaG044429 AT1G65620.4 54.167 120 52 1 8 127 9 125 6.13e-34 120
MsaG044429 AT1G65620.3 54.167 120 52 1 8 127 9 125 6.13e-34 120
MsaG044429 AT1G65620.5 54.167 120 52 1 8 127 9 125 6.13e-34 120
MsaG044429 AT1G65620.2 54.167 120 52 1 8 127 9 125 6.13e-34 120
MsaG044429 AT5G63090.4 50.962 104 51 0 8 111 11 114 1.11e-32 117
MsaG044429 AT5G63090.3 50.962 104 51 0 8 111 11 114 1.11e-32 117
MsaG044429 AT5G63090.2 50.962 104 51 0 8 111 11 114 1.11e-32 117
MsaG044429 AT5G63090.1 50.962 104 51 0 8 111 11 114 1.11e-32 117
MsaG044429 AT2G23660.2 52.336 107 51 0 8 114 5 111 2.59e-32 119
MsaG044429 AT2G23660.3 52.336 107 51 0 8 114 5 111 2.59e-32 119
MsaG044429 AT2G23660.1 52.336 107 51 0 8 114 5 111 2.59e-32 119
MsaG044429 AT1G07900.1 42.975 121 68 1 3 123 28 147 4.73e-30 110
MsaG044429 AT3G27650.1 47.115 104 55 0 8 111 39 142 8.51e-30 108
MsaG044429 AT2G28500.1 47.115 104 55 0 5 108 52 155 1.34e-29 110
MsaG044429 AT2G30130.1 46.535 101 54 0 8 108 8 108 1.40e-29 109
MsaG044429 AT1G16530.1 43.478 115 64 1 6 119 12 126 3.71e-29 107
MsaG044429 AT1G31320.1 50.000 88 44 0 8 95 13 100 5.19e-28 104
MsaG044429 AT2G40470.2 39.098 133 81 0 6 138 18 150 1.95e-26 101
MsaG044429 AT3G26660.1 41.584 101 59 0 9 109 6 106 4.36e-26 98.2
MsaG044429 AT2G30340.2 42.373 118 66 1 6 121 51 168 5.11e-26 102
MsaG044429 AT3G26620.1 41.584 101 59 0 9 109 6 106 5.17e-26 97.8
MsaG044429 AT2G30340.1 42.373 118 66 1 6 121 50 167 5.74e-26 102
MsaG044429 AT2G40470.1 39.098 133 81 0 6 138 43 175 7.31e-26 100
MsaG044429 AT3G11090.1 44.000 100 56 0 9 108 12 111 1.09e-25 98.6
MsaG044429 AT3G50510.1 42.342 111 63 1 1 111 6 115 4.27e-25 97.8
MsaG044429 AT3G50510.2 42.342 111 63 1 1 111 6 115 4.27e-25 97.8
MsaG044429 AT5G35900.1 43.269 104 59 0 9 112 6 109 1.33e-24 96.7
MsaG044429 AT3G47870.1 39.806 103 62 0 9 111 37 139 1.69e-23 96.3
MsaG044429 AT1G72980.1 39.000 100 61 0 9 108 14 113 1.47e-22 91.7
MsaG044429 AT3G03760.1 43.137 102 57 1 8 108 51 152 6.01e-22 91.3
MsaG044429 AT4G00210.1 43.689 103 57 1 8 109 11 113 6.06e-22 90.1
MsaG044429 AT2G42430.1 40.777 103 60 1 8 109 15 117 9.08e-22 90.1
MsaG044429 AT3G13850.1 37.864 103 64 0 9 111 37 139 1.54e-21 90.1
MsaG044429 AT3G58190.1 42.308 104 59 1 8 110 11 114 5.13e-21 87.8
MsaG044429 AT2G45420.1 43.000 100 56 1 11 109 40 139 2.39e-20 86.7
MsaG044429 AT2G42440.1 38.462 104 63 1 8 110 7 110 8.24e-20 85.1
MsaG044429 AT4G00220.1 44.000 100 55 1 11 109 20 119 1.13e-19 84.3
MsaG044429 AT2G45410.1 42.000 100 57 1 11 109 19 118 3.48e-18 79.3
MsaG044429 AT5G06080.1 29.333 150 89 2 8 140 7 156 5.17e-17 75.9
MsaG044429 AT2G31310.1 36.538 104 65 1 8 110 7 110 5.78e-17 76.3
MsaG044429 AT4G22700.1 36.364 110 68 2 9 117 6 114 6.19e-17 76.3
MsaG044429 AT1G06280.1 29.586 169 111 4 7 172 23 186 4.87e-14 68.6
MsaG044429 AT2G19820.1 28.972 107 64 3 8 113 12 107 7.25e-11 58.2

Find 83 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTTCCTGTTCATGATTC+TGG 0.107641 8:+60093885 MsaT044429.1:CDS
CTTCTGTGCTTCCTTCTTCT+AGG 0.193943 8:+60093955 MsaT044429.1:CDS
ATGTTCCTGTTCATGATTCT+GGG 0.297022 8:+60093886 MsaT044429.1:CDS
GTTCATGATTCTGGGTTGGC+TGG 0.362539 8:+60093894 MsaT044429.1:CDS
AAACGCTCCGGGTTGTCCTT+TGG 0.364971 8:-60093349 None:intergenic
GACCTGAGGATTGAGGTATT+TGG 0.365999 8:-60093587 None:intergenic
CTACCTTGAAGGTCATTTGC+TGG 0.366573 8:-60093517 None:intergenic
GCCAAGGTACACTCGGTGTT+TGG 0.371149 8:+60093373 MsaT044429.1:CDS
AGCAACCAGGGTCGATTGCC+GGG 0.393568 8:+60094011 MsaT044429.1:CDS
TGTACCTTGGCGAAACGCTC+CGG 0.395772 8:-60093361 None:intergenic
AAGCAACCAGGGTCGATTGC+CGG 0.397461 8:+60094010 MsaT044429.1:CDS
TTCTGTGCTTCCTTCTTCTA+GGG 0.409577 8:+60093956 MsaT044429.1:CDS
GAGGAACATGAGGAAGCATC+TGG 0.409895 8:-60093920 None:intergenic
CTAAAACAAAAGCTTCTTAG+TGG 0.418812 8:+60093753 MsaT044429.1:CDS
CAGGAACATCAGCACCCATC+TGG 0.437219 8:-60093872 None:intergenic
AGCACAGAAGGAGCATCAAC+AGG 0.442967 8:-60093942 None:intergenic
AGCTAGGGTGGCACTTCCTA+TGG 0.445033 8:-60093306 None:intergenic
TCCTGTTCATGATTCTGGGT+TGG 0.449021 8:+60093890 MsaT044429.1:CDS
CTTCATTCAACCTACCTTGA+AGG 0.452245 8:-60093528 None:intergenic
GCCAACCCATCATCACCATC+GGG 0.452840 8:-60093777 None:intergenic
GGGTTGTCCTTTGGAAAGTA+AGG 0.456967 8:-60093340 None:intergenic
AGTGGCCCCGATGGTGATGA+TGG 0.469173 8:+60093771 MsaT044429.1:CDS
GGTGGTTCAGTCGGTGGTAG+TGG 0.471163 8:+60093834 MsaT044429.1:CDS
CAGGAACATTAACATCCATC+TGG 0.471455 8:-60093806 None:intergenic
GACCCTATCCATGGTTGTGC+GGG 0.480158 8:+60093493 MsaT044429.1:CDS
GGCTCCCCGGCAATCGACCC+TGG 0.481884 8:-60094016 None:intergenic
GCCAACCCAGAATCATGAAC+AGG 0.484253 8:-60093891 None:intergenic
TGGTGTATGAAGCTGATGCC+TGG 0.491815 8:+60093464 MsaT044429.1:CDS
CCGACTGAACCACCACCAAC+AGG 0.496150 8:-60093825 None:intergenic
TAAGGAGCTAAGACACAGCT+AGG 0.498376 8:-60093322 None:intergenic
TGCCAACCCATCATCACCAT+CGG 0.501173 8:-60093778 None:intergenic
TGAACAAGCTGTTGAATCCT+TGG 0.502541 8:+60093444 MsaT044429.1:CDS
GTTAATGTTCCTGTTGGTGG+TGG 0.504572 8:+60093816 MsaT044429.1:CDS
GATGTTAATGTTCCTGTTGG+TGG 0.510237 8:+60093813 MsaT044429.1:CDS
TAGGTTGAATGAAGCTCAAA+CGG 0.515884 8:+60093537 MsaT044429.1:CDS
AAAGTATCAGCCCTAGAAGA+AGG 0.518742 8:-60093966 None:intergenic
GTACCTTGGCGAAACGCTCC+GGG 0.518769 8:-60093360 None:intergenic
CAAGAGTATGAAGAATGAAC+TGG 0.518901 8:+60093564 MsaT044429.1:CDS
GATGGTGATGATGGGTTGGC+AGG 0.524172 8:+60093780 MsaT044429.1:CDS
CTAGAAGAAGGAAGCACAGA+AGG 0.524800 8:-60093954 None:intergenic
GACCCGCACAACCATGGATA+GGG 0.528727 8:-60093495 None:intergenic
TTGCTGGACCCGCACAACCA+TGG 0.531067 8:-60093501 None:intergenic
CTTAGCTCCTTACTTTCCAA+AGG 0.539710 8:+60093333 MsaT044429.1:CDS
CCCCGATGGTGATGATGGGT+TGG 0.540092 8:+60093776 MsaT044429.1:CDS
GATCAAACGTTGTGGCTCCC+CGG 0.541997 8:-60094029 None:intergenic
ATGAAGCTGATGCCTGGTTG+AGG 0.542839 8:+60093470 MsaT044429.1:CDS
TCCAAACACCGAGTGTACCT+TGG 0.546759 8:-60093374 None:intergenic
GGTCCAGCAAATGACCTTCA+AGG 0.548176 8:+60093514 MsaT044429.1:CDS
AGTCGGTGGTAGTGGTGGCT+CGG 0.550407 8:+60093842 MsaT044429.1:CDS
GGCCAAATACCTCAATCCTC+AGG 0.557788 8:+60093585 MsaT044429.1:CDS
TGGCTCGGCAAGATTCCAGA+TGG 0.560308 8:+60093857 MsaT044429.1:CDS
GGACCCGCACAACCATGGAT+AGG 0.560372 8:-60093496 None:intergenic
CCTGTTGGTGGTGGTTCAGT+CGG 0.562105 8:+60093825 MsaT044429.1:CDS
AGATGCTTGCAAGCTGCACA+TGG 0.564745 8:-60093283 None:intergenic
GGACCCTATCCATGGTTGTG+CGG 0.567733 8:+60093492 MsaT044429.1:CDS
TAGCACTAGCACAAGCAACC+AGG 0.569645 8:+60093998 MsaT044429.1:CDS
TGAAGCTGATGCCTGGTTGA+GGG 0.570610 8:+60093471 MsaT044429.1:CDS
GGCTCGGCAAGATTCCAGAT+GGG 0.571382 8:+60093858 MsaT044429.1:CDS
ATGGATAGGGTCCCTCAACC+AGG 0.574346 8:-60093482 None:intergenic
ATTCAACGACCTGAGGATTG+AGG 0.583762 8:-60093594 None:intergenic
CAGCTTGCAAGCATCTCCAT+AGG 0.587034 8:+60093290 MsaT044429.1:CDS
AAGCTTCTTAGTGGCCCCGA+TGG 0.587409 8:+60093762 MsaT044429.1:CDS
ATGGATGTTAATGTTCCTGT+TGG 0.590588 8:+60093810 MsaT044429.1:CDS
TGGTTGAGGGACCCTATCCA+TGG 0.595646 8:+60093484 MsaT044429.1:CDS
GCAACCAGGGTCGATTGCCG+GGG 0.604916 8:+60094012 MsaT044429.1:CDS
GGTTCAGTCGGTGGTAGTGG+TGG 0.608000 8:+60093837 MsaT044429.1:CDS
GCATCAACAGGAGGAACATG+AGG 0.622008 8:-60093930 None:intergenic
AGGAACATGAGGAAGCATCT+GGG 0.622177 8:-60093919 None:intergenic
CGCCGCAAGATCAAACGTTG+TGG 0.625416 8:-60094037 None:intergenic
GAGCTAAGACACAGCTAGGG+TGG 0.629501 8:-60093318 None:intergenic
CAGCAAATGACCTTCAAGGT+AGG 0.629800 8:+60093518 MsaT044429.1:CDS
GTTGGTGGTGGTTCAGTCGG+TGG 0.631838 8:+60093828 MsaT044429.1:CDS
GCGTTTCGCCAAGGTACACT+CGG 0.633186 8:+60093366 MsaT044429.1:CDS
TTACTTTCCAAAGGACAACC+CGG 0.635497 8:+60093342 MsaT044429.1:CDS
AAGGAGCTAAGACACAGCTA+GGG 0.637825 8:-60093321 None:intergenic
GTGGCCCCGATGGTGATGAT+GGG 0.639305 8:+60093772 MsaT044429.1:CDS
CAACCCGGAGCGTTTCGCCA+AGG 0.640771 8:+60093357 MsaT044429.1:CDS
GATGCTTGCAAGCTGCACAT+GGG 0.660822 8:-60093282 None:intergenic
AGCACTAGCACAAGCAACCA+GGG 0.667332 8:+60093999 MsaT044429.1:CDS
TGGGTTGGCAGGATTCCAGA+TGG 0.675884 8:+60093791 MsaT044429.1:CDS
ACAGAAGGAGCATCAACAGG+AGG 0.677829 8:-60093939 None:intergenic
GGCATCAGCTTCATACACCA+AGG 0.679242 8:-60093461 None:intergenic
CCAACCCATCATCACCATCG+GGG 0.738435 8:-60093776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 60093262 60094049 60093262 ID=MsaG044429
Chr8 mRNA 60093262 60094049 60093262 ID=MsaT044429.1;Parent=MsaG044429
Chr8 exon 60093262 60093606 60093262 ID=MsaT044429.1.exon1;Parent=MsaT044429.1
Chr8 CDS 60093262 60093606 60093262 ID=cds.MsaT044429.1;Parent=MsaT044429.1
Chr8 exon 60093732 60094049 60093732 ID=MsaT044429.1.exon2;Parent=MsaT044429.1
Chr8 CDS 60093732 60094049 60093732 ID=cds.MsaT044429.1;Parent=MsaT044429.1
Gene Sequence

>MsaT044429.1

ATGGATAGAGAGATTATTCGCCCATGTGCAGCTTGCAAGCATCTCCATAGGAAGTGCCACCCTAGCTGTGTCTTAGCTCCTTACTTTCCAAAGGACAACCCGGAGCGTTTCGCCAAGGTACACTCGGTGTTTGGAAAGAGAAATGTGACTAATCTGTTGAAAAAACTGGATGTTTCACATCATGAACAAGCTGTTGAATCCTTGGTGTATGAAGCTGATGCCTGGTTGAGGGACCCTATCCATGGTTGTGCGGGTCCAGCAAATGACCTTCAAGGTAGGTTGAATGAAGCTCAAACGGAGCTCAAGAGTATGAAGAATGAACTGGCCAAATACCTCAATCCTCAGCAGCTTCACAGCCTAGAAGTTCTAAAACAAAAGCTTCTTAGTGGCCCCGATGGTGATGATGGGTTGGCAGGATTCCAGATGGATGTTAATGTTCCTGTTGGTGGTGGTTCAGTCGGTGGTAGTGGTGGCTCGGCAAGATTCCAGATGGGTGCTGATGTTCCTGTTCATGATTCTGGGTTGGCTGGATCCCAGATGCTTCCTCATGTTCCTCCTGTTGATGCTCCTTCTGTGCTTCCTTCTTCTAGGGCTGATACTTTTGACAATGGTAGCACTAGCACAAGCAACCAGGGTCGATTGCCGGGGAGCCACAACGTTTGA
Protein sequence

>MsaT044429.1

MDREIIRPCAACKHLHRKCHPSCVLAPYFPKDNPERFAKVHSVFGKRNVTNLLKKLDVSHHEQAVESLVYEADAWLRDPIHGCAGPANDLQGRLNEAQTELKSMKNELAKYLNPQQLHSLEVLKQKLLSGPDGDDGLAGFQMDVNVPVGGGSVGGSGGSARFQMGADVPVHDSGLAGSQMLPHVPPVDAPSVLPSSRADTFDNGSTSTSNQGRLPGSHNV*