AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044430 MtrunA17_Chr8g0374581 89.595 173 11 1 1 166 1 173 2.38e-111 316
MsaG044430 MtrunA17_Chr8g0374601 53.801 171 64 2 1 157 19 188 2.63e-55 175
MsaG044430 MtrunA17_Chr8g0374591 56.667 120 51 1 1 120 1 119 6.02e-44 141
MsaG044430 MtrunA17_Chr3g0115431 56.757 111 47 1 8 118 9 118 1.57e-38 130
MsaG044430 MtrunA17_Chr5g0402611 52.066 121 57 1 1 121 1 120 1.17e-37 131
MsaG044430 MtrunA17_Chr3g0097221 44.172 163 66 4 8 146 7 168 9.84e-36 125
MsaG044430 MtrunA17_Chr3g0106101 50.000 102 50 1 8 109 7 107 8.78e-33 114
MsaG044430 MtrunA17_Chr5g0437571 50.980 102 49 1 8 109 6 106 6.42e-32 112
MsaG044430 MtrunA17_Chr4g0058651 50.476 105 51 1 8 112 11 114 2.88e-31 111
MsaG044430 MtrunA17_Chr3g0114061 47.706 109 56 1 1 109 1 108 7.71e-31 109
MsaG044430 MtrunA17_Chr3g0117121 48.182 110 56 1 8 117 10 118 4.49e-30 108
MsaG044430 MtrunA17_Chr2g0311601 48.148 108 55 1 3 110 8 114 5.18e-30 107
MsaG044430 MtrunA17_Chr7g0227511 49.524 105 52 1 8 112 11 114 1.92e-29 106
MsaG044430 MtrunA17_Chr4g0030001 46.667 105 56 0 5 109 21 125 2.24e-29 106
MsaG044430 MtrunA17_Chr3g0129071 50.467 107 52 1 6 112 45 150 2.59e-29 107
MsaG044430 MtrunA17_Chr5g0438091 50.476 105 51 1 8 112 39 142 4.14e-29 105
MsaG044430 MtrunA17_Chr6g0449641 48.077 104 53 1 8 111 9 111 5.29e-28 101
MsaG044430 MtrunA17_Chr4g0043421 50.495 101 49 1 8 108 15 114 8.92e-28 102
MsaG044430 MtrunA17_Chr5g0432451 52.336 107 50 1 6 112 41 146 1.47e-27 102
MsaG044430 MtrunA17_Chr1g0200201 49.020 102 51 1 9 110 73 173 3.17e-27 102
MsaG044430 MtrunA17_Chr5g0444351 45.098 102 55 1 9 110 6 106 3.74e-27 99.4
MsaG044430 MtrunA17_Chr6g0449651 46.667 105 55 1 8 112 67 170 3.97e-27 100
MsaG044430 MtrunA17_Chr7g0225111 45.283 106 57 1 5 110 2 106 4.88e-27 98.2
MsaG044430 MtrunA17_Chr8g0353981 49.091 110 55 1 8 117 7 115 1.09e-25 98.2
MsaG044430 MtrunA17_Chr5g0437401 45.631 103 55 1 9 111 12 113 3.10e-25 95.5
MsaG044430 MtrunA17_Chr6g0460951 43.519 108 59 2 3 109 35 141 5.28e-24 93.6
MsaG044430 MtrunA17_Chr3g0113891 47.959 98 50 1 9 106 15 111 6.73e-24 92.4
MsaG044430 MtrunA17_Chr7g0260971 41.667 108 61 2 8 114 14 120 3.71e-21 85.9
MsaG044430 MtrunA17_Chr4g0055441 42.718 103 57 2 9 110 3 104 4.25e-21 84.3
MsaG044430 MtrunA17_Chr8g0351701 41.509 106 60 2 8 112 23 127 8.56e-21 85.5
MsaG044430 MtrunA17_Chr7g0245871 43.689 103 56 2 8 109 27 128 1.22e-20 85.1
MsaG044430 MtrunA17_Chr4g0040831 38.462 104 63 1 5 108 12 114 1.81e-20 84.0
MsaG044430 MtrunA17_Chr1g0184281 35.862 145 74 5 8 138 15 154 2.81e-20 83.6
MsaG044430 MtrunA17_Chr6g0452691 39.806 103 60 2 9 110 10 111 4.66e-19 79.7
MsaG044430 MtrunA17_Chr1g0184271 42.000 100 56 2 8 106 12 110 6.75e-19 80.5
MsaG044430 MtrunA17_Chr7g0261031 39.216 102 60 2 8 108 8 108 5.45e-18 77.8
MsaG044430 MtrunA17_Chr6g0460941 41.489 94 53 2 4 96 3 95 5.54e-18 74.3
MsaG044430 MtrunA17_Chr6g0452661 37.736 106 61 3 5 106 3 107 6.09e-18 78.2
MsaG044430 MtrunA17_Chr8g0351721 43.269 104 57 2 8 110 18 120 1.04e-17 77.0
MsaG044430 MtrunA17_Chr5g0439111 40.000 110 61 3 5 110 3 111 1.90e-17 76.6
MsaG044430 MtrunA17_Chr4g0059901 38.835 103 62 1 7 109 9 110 3.26e-17 76.3
MsaG044430 MtrunA17_Chr6g0452671 38.000 100 61 1 7 106 10 108 3.30e-17 75.1
MsaG044430 MtrunA17_Chr5g0435641 40.000 95 55 2 9 102 8 101 4.77e-17 74.7
MsaG044430 MtrunA17_Chr7g0245861 45.000 80 42 1 8 85 12 91 2.00e-15 68.9
MsaG044430 MtrunA17_Chr2g0330201 32.692 104 69 1 7 110 18 120 9.58e-14 67.4
MsaG044430 MtrunA17_Chr8g0342351 34.653 101 58 2 9 109 13 105 1.09e-13 66.6
MsaG044430 MtrunA17_Chr2g0326041 53.571 56 24 2 1 55 25 79 3.21e-13 62.0
MsaG044430 MtrunA17_Chr4g0046201 37.975 79 48 1 9 87 7 84 3.66e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044430 AT5G66870.1 49.606 127 62 2 8 134 7 131 6.22e-35 124
MsaG044430 AT2G23660.2 51.351 111 53 1 8 118 5 114 3.49e-34 122
MsaG044430 AT2G23660.3 51.351 111 53 1 8 118 5 114 3.49e-34 122
MsaG044430 AT2G23660.1 51.351 111 53 1 8 118 5 114 3.49e-34 122
MsaG044430 AT1G16530.1 50.000 104 52 0 6 109 12 115 3.30e-32 113
MsaG044430 AT2G30130.1 50.000 102 50 1 8 109 8 108 2.77e-30 109
MsaG044430 AT1G07900.1 48.113 106 54 1 5 110 30 134 2.89e-30 109
MsaG044430 AT2G28500.1 47.619 105 54 1 5 109 52 155 2.10e-29 108
MsaG044430 AT3G27650.1 48.571 105 53 1 8 112 39 142 3.01e-29 105
MsaG044430 AT5G63090.4 42.361 144 75 2 8 151 11 146 3.05e-29 106
MsaG044430 AT5G63090.3 42.361 144 75 2 8 151 11 146 3.05e-29 106
MsaG044430 AT5G63090.2 42.361 144 75 2 8 151 11 146 3.05e-29 106
MsaG044430 AT5G63090.1 42.361 144 75 2 8 151 11 146 3.05e-29 106
MsaG044430 AT2G40470.2 47.244 127 64 2 6 130 18 143 8.33e-29 105
MsaG044430 AT2G30340.2 46.281 121 62 2 6 124 51 170 1.06e-28 107
MsaG044430 AT2G30340.1 46.281 121 62 2 6 124 50 169 1.12e-28 107
MsaG044430 AT2G40470.1 47.244 127 64 2 6 130 43 168 2.35e-28 105
MsaG044430 AT1G31320.1 56.818 88 37 1 8 95 13 99 4.93e-28 102
MsaG044430 AT1G65620.1 54.286 105 47 1 8 112 9 112 6.01e-28 103
MsaG044430 AT1G65620.4 54.286 105 47 1 8 112 9 112 6.01e-28 103
MsaG044430 AT1G65620.3 54.286 105 47 1 8 112 9 112 6.01e-28 103
MsaG044430 AT1G65620.5 54.286 105 47 1 8 112 9 112 6.01e-28 103
MsaG044430 AT1G65620.2 54.286 105 47 1 8 112 9 112 6.01e-28 103
MsaG044430 AT3G26660.1 43.137 102 57 1 9 110 6 106 1.45e-25 95.1
MsaG044430 AT3G26620.1 43.137 102 57 1 9 110 6 106 1.65e-25 95.1
MsaG044430 AT3G11090.1 45.192 104 56 1 9 112 12 114 1.14e-23 91.7
MsaG044430 AT3G50510.1 41.818 110 63 1 3 112 7 115 2.01e-23 91.7
MsaG044430 AT3G50510.2 41.818 110 63 1 3 112 7 115 2.01e-23 91.7
MsaG044430 AT4G00210.1 45.192 104 55 2 8 110 11 113 6.58e-21 85.9
MsaG044430 AT3G03760.1 43.689 103 56 2 8 109 51 152 7.52e-21 86.7
MsaG044430 AT3G47870.1 38.462 104 63 1 9 112 37 139 8.34e-20 84.7
MsaG044430 AT2G42430.1 39.623 106 62 2 8 112 15 119 3.51e-19 81.6
MsaG044430 AT3G58190.1 40.952 105 60 2 8 111 11 114 5.95e-19 80.5
MsaG044430 AT2G42440.1 36.066 122 75 3 8 128 7 126 8.64e-19 80.5
MsaG044430 AT2G45410.1 38.732 142 63 5 11 151 19 137 1.28e-17 76.3
MsaG044430 AT5G35900.1 33.333 117 77 1 9 125 6 121 3.22e-17 75.9
MsaG044430 AT4G00220.1 40.594 101 58 2 11 110 20 119 3.99e-16 73.2
MsaG044430 AT2G45420.1 38.235 102 61 2 11 111 40 140 1.06e-15 72.8
MsaG044430 AT1G06280.1 42.500 80 45 1 7 86 23 101 5.11e-15 69.7
MsaG044430 AT3G13850.1 33.019 106 70 1 9 114 37 141 6.62e-15 70.5
MsaG044430 AT5G06080.1 35.849 106 66 2 8 112 7 111 8.88e-15 68.6
MsaG044430 AT1G72980.1 36.275 102 64 1 8 109 13 113 1.03e-14 69.3
MsaG044430 AT2G31310.1 34.286 105 67 2 8 111 7 110 7.18e-14 66.6

Find 39 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTGAGGGACAATGTTTA+TGG 0.185300 8:+60120217 MsaT044430.1:CDS
CAAACAAGTCGCTGATTCTT+TGG 0.280466 8:+60120177 MsaT044430.1:CDS
GTCTTGTTCTTGCAATAGTT+TGG 0.290230 8:-60120143 None:intergenic
CTGTCCAATCCTGATCGTTT+CGG 0.322703 8:+60120355 MsaT044430.1:CDS
CGCTGATTCTTTGGTGTATG+AGG 0.387590 8:+60120186 MsaT044430.1:CDS
CTCTGAAAGAAAAGATTGTT+AGG 0.410113 8:+60120458 MsaT044430.1:CDS
CTTTCTTGAAAAGACATTGA+AGG 0.413713 8:+60120258 MsaT044430.1:CDS
AACCTCGTACACTTGGTGTT+TGG 0.420273 8:+60120106 MsaT044430.1:CDS
GGTGCAAAGCCGAAACGATC+AGG 0.465489 8:-60120364 None:intergenic
AAACGTTGTGGGTTGTCCGC+TGG 0.468485 8:-60120082 None:intergenic
TGAGGCCGAGGCACGTTTGA+GGG 0.480357 8:+60120204 MsaT044430.1:CDS
GGGTTGTCCGCTGGAAAGTA+AGG 0.487972 8:-60120073 None:intergenic
GACAATGTTTATGGATGTGT+TGG 0.490676 8:+60120226 MsaT044430.1:CDS
TGATGCTTGCACGCTGCACA+CGG 0.493660 8:-60120013 None:intergenic
AGAACCTCTTGAATGATCTC+AGG 0.498563 8:-60120334 None:intergenic
AATAGAGTTGATCACGTGTC+GGG 0.500142 8:-60120481 None:intergenic
CTTGAATGATCTCAGGATCA+AGG 0.520815 8:-60120327 None:intergenic
TTCTTTGGTGTATGAGGCCG+AGG 0.523568 8:+60120192 MsaT044430.1:CDS
AATCAGCGACTTGTTTGCGA+TGG 0.533026 8:-60120171 None:intergenic
ATGAGGCCGAGGCACGTTTG+AGG 0.549695 8:+60120203 MsaT044430.1:CDS
CGTGCAAGCATCATCGTCGA+AGG 0.549844 8:+60120023 MsaT044430.1:CDS
ACGTTTCAACCTCGTACACT+TGG 0.555564 8:+60120099 MsaT044430.1:CDS
GTCAACTGATCAAGAGTTGG+CGG 0.563316 8:-60120430 None:intergenic
TGTACGAGGTTGAAACGTTG+TGG 0.572380 8:-60120094 None:intergenic
ATCAGCGACTTGTTTGCGAT+GGG 0.575116 8:-60120170 None:intergenic
GTCGTCAACTGATCAAGAGT+TGG 0.576558 8:-60120433 None:intergenic
ATTGTCCCTCAAACGTGCCT+CGG 0.577681 8:-60120209 None:intergenic
TGATCCTGAGATCATTCAAG+AGG 0.583673 8:+60120330 MsaT044430.1:CDS
AAAGCCGAAACGATCAGGAT+TGG 0.585751 8:-60120359 None:intergenic
TGTTCAAGATGAACTCAAGA+AGG 0.590687 8:+60120282 MsaT044430.1:CDS
TCGTACACTTGGTGTTTGGA+AGG 0.594785 8:+60120110 MsaT044430.1:CDS
GTACGAGGTTGAAACGTTGT+GGG 0.597027 8:-60120093 None:intergenic
CAATAGAGTTGATCACGTGT+CGG 0.598555 8:-60120482 None:intergenic
CCGCTGGAAAGTAAGGAGCT+AGG 0.607576 8:-60120066 None:intergenic
GATGCTTGCACGCTGCACAC+GGG 0.622773 8:-60120012 None:intergenic
CCTAGCTCCTTACTTTCCAG+CGG 0.652064 8:+60120066 MsaT044430.1:CDS
TGAGAAACAAGATTTCCTTG+TGG 0.660079 8:-60120403 None:intergenic
TTCCAAACACCAAGTGTACG+AGG 0.663049 8:-60120108 None:intergenic
AGCCCTAATCAAAATCCACA+AGG 0.702653 8:+60120388 MsaT044430.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 60119992 60120492 60119992 ID=MsaG044430
Chr8 mRNA 60119992 60120492 60119992 ID=MsaT044430.1;Parent=MsaG044430
Chr8 exon 60119992 60120492 60119992 ID=MsaT044430.1.exon1;Parent=MsaT044430.1
Chr8 CDS 60119992 60120492 60119992 ID=cds.MsaT044430.1;Parent=MsaT044430.1
Gene Sequence

>MsaT044430.1

ATGGCAGAAGAGATTATTCGCCCGTGTGCAGCGTGCAAGCATCATCGTCGAAGGTGCGACGAAACAAGCTGCAACCTAGCTCCTTACTTTCCAGCGGACAACCCACAACGTTTCAACCTCGTACACTTGGTGTTTGGAAGGAGCAAAGTGTCCAAACTATTGCAAGAACAAGACATTTCCCATCGCAAACAAGTCGCTGATTCTTTGGTGTATGAGGCCGAGGCACGTTTGAGGGACAATGTTTATGGATGTGTTGGTTCTGCATCCTTTCTTGAAAAGACATTGAAGGATGTTCAAGATGAACTCAAGAAGGTGAAGAATGAACTGACTAAATACCTTGATCCTGAGATCATTCAAGAGGTTCTGTCCAATCCTGATCGTTTCGGCTTTGCACCAAGCCCTAATCAAAATCCACAAGGAAATCTTGTTTCTCAGCAGCCGCCAACTCTTGATCAGTTGACGACTTCTCTGAAAGAAAAGATTGTTAGGCCCGACACGTGA
Protein sequence

>MsaT044430.1

MAEEIIRPCAACKHHRRRCDETSCNLAPYFPADNPQRFNLVHLVFGRSKVSKLLQEQDISHRKQVADSLVYEAEARLRDNVYGCVGSASFLEKTLKDVQDELKKVKNELTKYLDPEIIQEVLSNPDRFGFAPSPNQNPQGNLVSQQPPTLDQLTTSLKEKIVRPDT*