AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044434


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044434 MtrunA17_Chr8g0374601 81.439 264 47 2 1 262 19 282 2.14e-148 416
MsaG044434 MtrunA17_Chr8g0374591 66.949 118 37 1 1 116 1 118 2.22e-50 161
MsaG044434 MtrunA17_Chr8g0374581 47.399 173 83 5 1 169 1 169 1.64e-43 147
MsaG044434 MtrunA17_Chr3g0097221 47.826 115 57 3 8 119 7 121 1.17e-27 107
MsaG044434 MtrunA17_Chr5g0402611 43.363 113 62 2 8 118 8 120 2.84e-26 104
MsaG044434 MtrunA17_Chr3g0115431 42.727 110 61 2 8 115 9 118 6.59e-25 99.0
MsaG044434 MtrunA17_Chr6g0449651 37.500 104 63 2 8 109 67 170 5.82e-19 82.4
MsaG044434 MtrunA17_Chr4g0058651 38.462 104 62 2 8 109 11 114 7.81e-19 81.6
MsaG044434 MtrunA17_Chr7g0227511 36.792 106 65 2 8 111 11 116 8.13e-19 81.3
MsaG044434 MtrunA17_Chr2g0311601 39.806 103 60 2 6 106 11 113 4.22e-18 79.0
MsaG044434 MtrunA17_Chr3g0106101 37.624 101 61 2 8 106 7 107 7.24e-18 79.0
MsaG044434 MtrunA17_Chr5g0437571 40.594 101 58 2 8 106 6 106 7.51e-18 78.6
MsaG044434 MtrunA17_Chr3g0117121 38.462 104 62 2 8 109 10 113 7.53e-18 79.3
MsaG044434 MtrunA17_Chr3g0114061 39.604 101 59 2 8 106 8 108 8.80e-18 78.2
MsaG044434 MtrunA17_Chr6g0449641 37.864 103 62 2 8 108 9 111 1.02e-17 77.8
MsaG044434 MtrunA17_Chr8g0353981 46.479 71 37 1 8 77 7 77 2.52e-17 78.6
MsaG044434 MtrunA17_Chr4g0043421 37.302 126 74 4 8 131 15 137 3.48e-17 77.0
MsaG044434 MtrunA17_Chr4g0030001 35.938 128 64 5 4 113 20 147 4.17e-17 77.0
MsaG044434 MtrunA17_Chr4g0055441 39.806 103 57 3 9 107 3 104 1.63e-16 74.7
MsaG044434 MtrunA17_Chr5g0438091 37.143 105 57 2 8 103 39 143 2.50e-16 75.1
MsaG044434 MtrunA17_Chr3g0129071 37.736 106 64 2 6 109 45 150 2.73e-16 75.9
MsaG044434 MtrunA17_Chr5g0432451 37.815 119 64 3 6 114 41 159 1.68e-15 73.6
MsaG044434 MtrunA17_Chr6g0460941 41.772 79 43 3 1 77 1 78 6.70e-15 68.6
MsaG044434 MtrunA17_Chr5g0444351 31.304 115 70 2 4 109 1 115 1.95e-14 68.9
MsaG044434 MtrunA17_Chr1g0200201 36.000 100 62 2 9 106 73 172 2.12e-14 70.5
MsaG044434 MtrunA17_Chr7g0225111 33.962 106 68 2 4 107 1 106 2.22e-14 67.8
MsaG044434 MtrunA17_Chr6g0460951 33.333 105 60 3 5 99 37 141 1.20e-13 68.6
MsaG044434 MtrunA17_Chr8g0342351 32.039 103 68 1 9 109 13 115 2.29e-13 68.2
MsaG044434 MtrunA17_Chr5g0437401 36.275 102 63 2 9 108 12 113 3.12e-13 66.2
MsaG044434 MtrunA17_Chr4g0040831 32.407 108 66 2 7 113 14 115 4.01e-13 66.6
MsaG044434 MtrunA17_Chr2g0326041 51.064 47 23 0 3 49 28 74 4.94e-13 63.5
MsaG044434 MtrunA17_Chr1g0184281 33.083 133 73 4 8 124 15 147 7.38e-13 65.9
MsaG044434 MtrunA17_Chr6g0452671 31.897 116 66 4 7 111 10 123 2.36e-12 64.3
MsaG044434 MtrunA17_Chr6g0452691 41.538 65 37 1 9 72 10 74 3.40e-12 63.5
MsaG044434 MtrunA17_Chr6g0452661 36.986 73 42 2 5 73 3 75 2.17e-11 62.4
MsaG044434 MtrunA17_Chr2g0330201 36.364 66 42 0 7 72 18 83 2.53e-11 62.8
MsaG044434 MtrunA17_Chr3g0113891 41.429 70 40 1 9 77 15 84 6.25e-11 60.1
MsaG044434 MtrunA17_Chr4g0059901 37.879 66 41 0 7 72 9 74 9.19e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044434 AT5G66870.1 42.982 114 63 2 8 119 7 120 1.56e-23 97.4
MsaG044434 AT2G23660.2 42.727 110 61 2 8 115 5 114 4.19e-22 93.6
MsaG044434 AT2G23660.3 42.727 110 61 2 8 115 5 114 4.19e-22 93.6
MsaG044434 AT2G23660.1 42.727 110 61 2 8 115 5 114 4.19e-22 93.6
MsaG044434 AT3G26660.1 38.614 101 55 2 9 108 6 100 6.55e-20 83.2
MsaG044434 AT3G26620.1 38.614 101 55 2 9 108 6 100 7.50e-20 83.2
MsaG044434 AT5G63090.4 39.423 104 61 2 8 109 11 114 3.18e-19 83.2
MsaG044434 AT5G63090.3 39.423 104 61 2 8 109 11 114 3.18e-19 83.2
MsaG044434 AT5G63090.2 39.423 104 61 2 8 109 11 114 3.18e-19 83.2
MsaG044434 AT5G63090.1 39.423 104 61 2 8 109 11 114 3.18e-19 83.2
MsaG044434 AT1G65620.1 43.396 106 58 2 8 111 9 114 5.96e-19 82.8
MsaG044434 AT1G65620.4 43.396 106 58 2 8 111 9 114 5.96e-19 82.8
MsaG044434 AT1G65620.3 43.396 106 58 2 8 111 9 114 5.96e-19 82.8
MsaG044434 AT1G65620.5 43.396 106 58 2 8 111 9 114 5.96e-19 82.8
MsaG044434 AT1G65620.2 43.396 106 58 2 8 111 9 114 5.96e-19 82.8
MsaG044434 AT2G30130.1 40.594 101 58 2 8 106 8 108 1.15e-18 82.0
MsaG044434 AT1G07900.1 36.792 106 58 3 3 99 28 133 3.57e-18 80.5
MsaG044434 AT3G27650.1 38.462 104 62 2 8 109 39 142 6.39e-18 79.0
MsaG044434 AT2G28500.1 37.143 105 57 3 4 99 51 155 1.42e-17 79.7
MsaG044434 AT1G16530.1 37.500 104 62 3 6 106 12 115 1.66e-16 75.5
MsaG044434 AT2G30340.2 36.792 106 65 2 6 109 51 156 3.01e-16 76.6
MsaG044434 AT1G31320.1 46.479 71 37 1 8 77 13 83 3.04e-16 74.7
MsaG044434 AT2G30340.1 36.792 106 65 2 6 109 50 155 3.55e-16 76.6
MsaG044434 AT2G40470.2 37.069 116 71 2 6 119 18 133 1.47e-15 73.6
MsaG044434 AT3G50510.1 34.483 116 56 2 5 109 9 115 3.59e-15 72.4
MsaG044434 AT3G50510.2 34.483 116 56 2 5 109 9 115 3.59e-15 72.4
MsaG044434 AT2G40470.1 37.069 116 71 2 6 119 43 158 3.86e-15 72.8
MsaG044434 AT1G72980.1 34.000 100 64 1 9 106 14 113 7.04e-15 72.0
MsaG044434 AT5G35900.1 31.731 104 69 1 9 110 6 109 1.12e-12 65.9
MsaG044434 AT3G47870.1 33.333 105 68 1 9 111 37 141 1.72e-12 66.6
MsaG044434 AT3G03760.1 33.636 110 63 4 8 107 51 160 6.67e-12 64.7
MsaG044434 AT3G11090.1 33.000 100 65 2 9 106 12 111 1.05e-11 62.4
MsaG044434 AT1G06280.1 36.364 66 42 0 7 72 23 88 9.02e-11 60.5

Find 70 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTTCTTGTTCGTGATTC+TGG 0.092323 8:-60184274 MsaT044434.1:CDS
ATCTCTCTTATTATTCTTGT+TGG 0.231044 8:+60184149 None:intergenic
TACCTTGAAGGTCATTTCTT+TGG 0.258996 8:+60184683 None:intergenic
CTCGCCTTTCCTGATTGTTT+CGG 0.296820 8:-60184577 MsaT044434.1:CDS
ATGTTCTTGTTCGTGATTCT+GGG 0.333393 8:-60184273 MsaT044434.1:CDS
ATCAAGGTACACTCTGTGTT+TGG 0.360225 8:-60184820 MsaT044434.1:CDS
TGAACAAGTTATTGAATCAT+TGG 0.367700 8:-60184749 MsaT044434.1:CDS
GACCTGAGGACTGAGGTATT+TGG 0.383512 8:+60184612 None:intergenic
TTAAAACAAAAGCTTCTTAG+TGG 0.403319 8:-60184442 MsaT044434.1:CDS
ATAGTATCAGCCCTAGAAAA+AGG 0.404708 8:+60184193 None:intergenic
AGATGCTTGCATGCTGCGCA+CGG 0.410315 8:+60184910 None:intergenic
TTGACAGAACCACCACCAAT+AGG 0.414297 8:+60184334 None:intergenic
GTTCGTGATTCTGGGTTGGC+AGG 0.416595 8:-60184265 MsaT044434.1:CDS
GGCTTAAAGCCGAAACAATC+AGG 0.421196 8:+60184568 None:intergenic
GAGGAACGTCGGGAAGCATA+TGG 0.427266 8:+60184239 None:intergenic
ATAGTCGATGCAGATGCATT+TGG 0.432023 8:+60184547 None:intergenic
GAGGCGGGTTGAGGACTCTA+TGG 0.432182 8:-60184716 MsaT044434.1:CDS
CAAGAACATCAGCACCCATC+TGG 0.437219 8:+60184287 None:intergenic
ATGAAACGCTCCGGATTGTC+AGG 0.439135 8:+60184841 None:intergenic
ATTGGTGGTGGTTCTGTCAA+TGG 0.446383 8:-60184331 MsaT044434.1:CDS
GTATGAGTCGAGGCGGGTTG+AGG 0.446873 8:-60184725 MsaT044434.1:CDS
AGCATGCAAGCATCTCCGTC+AGG 0.456773 8:-60184902 MsaT044434.1:CDS
CTTCATTCAACCTACCTTGA+AGG 0.457276 8:+60184671 None:intergenic
AGTACTAAGTCCCACCCTAT+TGG 0.458721 8:-60184349 MsaT044434.1:CDS
GACAGTCGATGCAAGACAAT+TGG 0.458838 8:-60184524 MsaT044434.1:CDS
AGTGGCCCCGATGGTGATGA+TGG 0.469173 8:-60184424 MsaT044434.1:CDS
CAGGAACATTAACATCCATC+TGG 0.471455 8:+60184389 None:intergenic
GATGGTGATGATGGGTTAGC+AGG 0.475686 8:-60184415 MsaT044434.1:CDS
GTCCAAAGAAATGACCTTCA+AGG 0.477311 8:-60184685 MsaT044434.1:CDS
TGACAGAACCACCACCAATA+GGG 0.490016 8:+60184335 None:intergenic
TTACTTTCCACCTGACAATC+CGG 0.490172 8:-60184851 MsaT044434.1:CDS
GGATTGTCAGGTGGAAAGTA+AGG 0.497768 8:+60184853 None:intergenic
GCATCAACATGAGGAACGTC+GGG 0.507797 8:+60184229 None:intergenic
GCTAACCCATCATCACCATC+GGG 0.507921 8:+60184418 None:intergenic
GGCCAAATACCTCAGTCCTC+AGG 0.517957 8:-60184614 MsaT044434.1:CDS
TGCTAACCCATCATCACCAT+CGG 0.521414 8:+60184417 None:intergenic
GATGCTTGCATGCTGCGCAC+GGG 0.523329 8:+60184911 None:intergenic
GTTAATGTTCCTGTTGAAAA+CGG 0.528434 8:-60184379 MsaT044434.1:CDS
AGGAACGTCGGGAAGCATAT+GGG 0.553787 8:+60184240 None:intergenic
TGAGTCGTCAACTCAGCAGC+TGG 0.557390 8:+60184487 None:intergenic
AAGTCCCACCCTATTGGTGG+TGG 0.560438 8:-60184343 MsaT044434.1:CDS
CAAAAGTGTGAAGAACGAAC+TGG 0.569336 8:-60184635 MsaT044434.1:CDS
ACTAAGTCCCACCCTATTGG+TGG 0.579669 8:-60184346 MsaT044434.1:CDS
CAATCCGGAGCGTTTCATCA+AGG 0.580538 8:-60184836 MsaT044434.1:CDS
TCTTGTTCGTGATTCTGGGT+TGG 0.583224 8:-60184269 MsaT044434.1:CDS
AAGCTTCTTAGTGGCCCCGA+TGG 0.587409 8:-60184433 MsaT044434.1:CDS
TGACTCGGCAAGATTCCAGA+TGG 0.587492 8:-60184302 MsaT044434.1:CDS
AGCATCAACATGAGGAACGT+CGG 0.589738 8:+60184228 None:intergenic
TGTCAATGGCAGTAGTGACT+CGG 0.589855 8:-60184317 MsaT044434.1:CDS
AGCTTGAGTCGCACTCCTGA+CGG 0.594185 8:+60184887 None:intergenic
ATTGGTGTATGAGTCGAGGC+GGG 0.595725 8:-60184731 MsaT044434.1:CDS
TGTACCTTGATGAAACGCTC+CGG 0.604580 8:+60184832 None:intergenic
CAGAACCACCACCAATAGGG+TGG 0.611871 8:+60184338 None:intergenic
GACTCGGCAAGATTCCAGAT+GGG 0.616618 8:-60184301 MsaT044434.1:CDS
TAGGTTGAATGAAGTTCGAA+CGG 0.624296 8:-60184662 MsaT044434.1:CDS
AAAGAAATGACCTTCAAGGT+AGG 0.624918 8:-60184681 MsaT044434.1:CDS
AAAGCCGAAACAATCAGGAA+AGG 0.640067 8:+60184573 None:intergenic
CATTGGTGTATGAGTCGAGG+CGG 0.642100 8:-60184732 MsaT044434.1:CDS
GTCGTCAACTCAGCAGCTGG+CGG 0.642102 8:+60184490 None:intergenic
TGGGTTAGCAGGATTCCAGA+TGG 0.646029 8:-60184404 MsaT044434.1:CDS
AAACGCTCCGGATTGTCAGG+TGG 0.648048 8:+60184844 None:intergenic
AGAACCACCACCAATAGGGT+GGG 0.649851 8:+60184339 None:intergenic
GTGGCCCCGATGGTGATGAT+GGG 0.652149 8:-60184423 MsaT044434.1:CDS
AATCATTGGTGTATGAGTCG+AGG 0.661385 8:-60184735 MsaT044434.1:CDS
AGAACAAATTGAACGACCTG+AGG 0.665667 8:+60184598 None:intergenic
AGGGCTGATACTATTGACAA+TGG 0.685373 8:-60184184 MsaT044434.1:CDS
ACAGAAGGAGCATCAACATG+AGG 0.691100 8:+60184220 None:intergenic
ATTGAACGACCTGAGGACTG+AGG 0.711426 8:+60184605 None:intergenic
AATGTTCCTGTTGAAAACGG+TGG 0.735325 8:-60184376 MsaT044434.1:CDS
CTAACCCATCATCACCATCG+GGG 0.785753 8:+60184419 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 60184159 60184953 60184159 ID=MsaG044434
Chr8 mRNA 60184159 60184953 60184159 ID=MsaT044434.1;Parent=MsaG044434
Chr8 exon 60184159 60184953 60184159 ID=MsaT044434.1.exon1;Parent=MsaT044434.1
Chr8 CDS 60184159 60184953 60184159 ID=cds.MsaT044434.1;Parent=MsaT044434.1
Gene Sequence

>MsaT044434.1

ATGGCAAGAGTGATTATTCGCCCGTGCGCAGCATGCAAGCATCTCCGTCAGGAGTGCGACTCAAGCTGTGACGTTGCTCCTTACTTTCCACCTGACAATCCGGAGCGTTTCATCAAGGTACACTCTGTGTTTGGAAAGAACAATGTGTCGAATATGTTCAAAAAACTTGATGTTTCACATCATGAACAAGTTATTGAATCATTGGTGTATGAGTCGAGGCGGGTTGAGGACTCTATGGTCATGCGAGTCCAAAGAAATGACCTTCAAGGTAGGTTGAATGAAGTTCGAACGGAACTCAAAAGTGTGAAGAACGAACTGGCCAAATACCTCAGTCCTCAGGTCGTTCAATTTGTTCTCGCCTTTCCTGATTGTTTCGGCTTTAAGCCAAATGCATCTGCATCGACTATGACAGTCGATGCAAGACAATTGGCTTTTAATCAGCCGCCAGCTGCTGAGTTGACGACTCAACAGATTCACAACTTAGAAGTGTTAAAACAAAAGCTTCTTAGTGGCCCCGATGGTGATGATGGGTTAGCAGGATTCCAGATGGATGTTAATGTTCCTGTTGAAAACGGTGGCACTAGTACTAAGTCCCACCCTATTGGTGGTGGTTCTGTCAATGGCAGTAGTGACTCGGCAAGATTCCAGATGGGTGCTGATGTTCTTGTTCGTGATTCTGGGTTGGCAGGATCCCATATGCTTCCCGACGTTCCTCATGTTGATGCTCCTTCTGTGCTTCCTTTTTCTAGGGCTGATACTATTGACAATGGTGATACTAGCAACCAACAAGAATAA

Protein sequence

>MsaT044434.1

MARVIIRPCAACKHLRQECDSSCDVAPYFPPDNPERFIKVHSVFGKNNVSNMFKKLDVSHHEQVIESLVYESRRVEDSMVMRVQRNDLQGRLNEVRTELKSVKNELAKYLSPQVVQFVLAFPDCFGFKPNASASTMTVDARQLAFNQPPAAELTTQQIHNLEVLKQKLLSGPDGDDGLAGFQMDVNVPVENGGTSTKSHPIGGGSVNGSSDSARFQMGADVLVRDSGLAGSHMLPDVPHVDAPSVLPFSRADTIDNGDTSNQQE*