AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044473


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044473 MtrunA17_Chr8g0375041 88.417 259 27 2 1 259 1 256 5.37e-170 469
MsaG044473 MtrunA17_Chr5g0402291 49.248 266 107 5 1 248 1 256 6.80e-77 234
MsaG044473 MtrunA17_Chr7g0257331 48.795 166 79 1 3 168 16 175 1.10e-47 158
MsaG044473 MtrunA17_Chr3g0134031 41.014 217 82 4 2 175 17 230 2.89e-47 161
MsaG044473 MtrunA17_Chr5g0397881 43.719 199 83 3 2 171 17 215 3.85e-47 160
MsaG044473 MtrunA17_Chr5g0441531 38.961 231 124 4 2 221 18 242 3.46e-46 155
MsaG044473 MtrunA17_Chr1g0203601 43.850 187 93 2 2 176 11 197 2.38e-42 146
MsaG044473 MtrunA17_Chr6g0472421 40.000 205 93 1 2 176 26 230 1.15e-41 145
MsaG044473 MtrunA17_Chr5g0410881 45.509 167 83 1 6 172 29 187 8.86e-41 146
MsaG044473 MtrunA17_Chr4g0039891 47.742 155 72 2 9 163 12 157 5.06e-39 141
MsaG044473 MtrunA17_Chr7g0260331 61.458 96 37 0 5 100 45 140 8.71e-37 133
MsaG044473 MtrunA17_Chr6g0467041 60.215 93 37 0 8 100 21 113 2.09e-36 131
MsaG044473 MtrunA17_Chr1g0204741 41.317 167 82 3 7 173 38 188 6.58e-36 130
MsaG044473 MtrunA17_Chr6g0485571 57.000 100 43 0 1 100 3 102 1.19e-35 130
MsaG044473 MtrunA17_Chr1g0185131 56.881 109 46 1 5 112 28 136 1.29e-35 129
MsaG044473 MtrunA17_Chr8g0392001 59.406 101 40 1 7 106 20 120 1.75e-35 130
MsaG044473 MtrunA17_Chr8g0379701 38.333 180 106 3 9 186 12 188 3.48e-35 129
MsaG044473 MtrunA17_Chr3g0135831 40.884 181 95 4 5 176 42 219 1.52e-34 127
MsaG044473 MtrunA17_Chr2g0284481 39.884 173 87 3 7 175 13 172 2.99e-34 127
MsaG044473 MtrunA17_Chr4g0071051 57.895 95 40 0 5 99 97 191 2.29e-33 125
MsaG044473 MtrunA17_Chr4g0047421 36.413 184 109 2 6 185 30 209 4.92e-33 119
MsaG044473 MtrunA17_Chr8g0378061 58.242 91 38 0 10 100 23 113 5.68e-32 122
MsaG044473 MtrunA17_Chr2g0330821 53.763 93 43 0 8 100 78 170 1.93e-29 113
MsaG044473 MtrunA17_Chr1g0179321 58.730 63 26 0 6 68 46 108 4.94e-23 90.5
MsaG044473 MtrunA17_Chr4g0023771 56.667 60 26 0 7 66 13 72 1.13e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044473 AT4G36990.1 45.492 244 114 5 2 227 7 249 1.91e-59 190
MsaG044473 AT1G46264.1 43.333 210 87 4 6 183 31 240 2.76e-45 155
MsaG044473 AT2G41690.1 45.810 179 85 3 6 172 37 215 9.93e-44 148
MsaG044473 AT4G11660.1 35.448 268 122 4 2 219 52 318 1.07e-42 149
MsaG044473 AT3G02990.1 45.665 173 80 3 6 178 20 178 7.70e-41 146
MsaG044473 AT5G62020.1 38.318 214 108 5 2 193 16 227 8.65e-41 142
MsaG044473 AT5G16820.1 66.316 95 32 0 6 100 24 118 1.55e-39 143
MsaG044473 AT5G16820.2 66.316 95 32 0 6 100 24 118 1.55e-39 143
MsaG044473 AT1G32330.1 46.707 167 83 3 6 172 34 194 2.29e-39 142
MsaG044473 AT1G32330.2 46.707 167 83 3 6 172 34 194 2.40e-39 142
MsaG044473 AT4G17750.1 63.158 95 35 0 7 101 50 144 4.10e-38 139
MsaG044473 AT3G24520.1 42.169 166 75 4 8 171 16 162 1.49e-37 135
MsaG044473 AT3G51910.1 52.101 119 48 1 6 124 26 135 3.79e-37 132
MsaG044473 AT2G26150.1 42.286 175 93 2 9 176 44 217 8.51e-37 133
MsaG044473 AT2G26150.4 42.286 175 93 2 9 176 44 217 8.51e-37 133
MsaG044473 AT3G22830.1 62.366 93 35 0 9 101 61 153 1.50e-35 131
MsaG044473 AT5G03720.2 60.638 94 37 0 8 101 54 147 2.72e-34 128
MsaG044473 AT5G03720.1 60.638 94 37 0 8 101 54 147 2.72e-34 128
MsaG044473 AT5G43840.1 40.994 161 80 1 3 163 13 158 3.02e-34 125
MsaG044473 AT3G63350.1 60.870 92 36 0 8 99 27 118 3.50e-34 125
MsaG044473 AT1G67970.1 43.750 160 74 3 7 165 17 161 2.72e-33 124
MsaG044473 AT4G13980.1 41.104 163 75 2 10 172 24 165 4.43e-33 125
MsaG044473 AT5G54070.1 55.435 92 41 0 8 99 70 161 1.26e-32 122
MsaG044473 AT5G45710.3 42.484 153 71 2 6 157 10 146 1.63e-30 116
MsaG044473 AT5G45710.1 42.484 153 71 2 6 157 10 146 1.63e-30 116
MsaG044473 AT4G18880.1 43.506 154 69 2 6 157 12 149 3.23e-30 117
MsaG044473 AT4G18870.1 40.659 91 54 0 6 96 143 233 1.18e-18 83.6
MsaG044473 AT4G18870.1 38.462 91 55 1 6 96 10 99 6.33e-15 73.2
MsaG044473 AT1G77570.1 39.080 87 50 2 10 93 27 113 2.34e-12 63.5

Find 78 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGGTGCTGCTGAAGATA+TGG 0.236484 8:-60740042 MsaT044473.1:CDS
TTGTTGTGCTTGAAATATTT+TGG 0.239756 8:+60743831 None:intergenic
TCACAAATGATGTGATTTCT+TGG 0.292322 8:-60743914 MsaT044473.1:CDS
CGAGAACAAAAGACTCAAAA+TGG 0.305997 8:-60739976 MsaT044473.1:CDS
ATTTGTCGGCAGCAGCTTTC+CGG 0.311222 8:+60740189 None:intergenic
CCCCTCTCTTAAAATTCTCT+TGG 0.321950 8:+60740156 None:intergenic
TTTCAATTTCTCAGGGGTTC+CGG 0.336163 8:-60740208 MsaT044473.1:intron
CACAAATGATGTGATTTCTT+GGG 0.344507 8:-60743913 MsaT044473.1:CDS
GAAAGGGTATAAGACTGTTT+GGG 0.345034 8:-60739767 MsaT044473.1:CDS
GTATAAGACTGTTTGGGGTT+TGG 0.352030 8:-60739761 MsaT044473.1:CDS
TTCAGGGCGGTCTCAACATC+GGG 0.365918 8:-60739887 MsaT044473.1:CDS
AGAATTGCTTGCTTATCTTC+AGG 0.372663 8:-60739904 MsaT044473.1:CDS
TCATTGTTGGCACCATCAAT+TGG 0.373020 8:+60739660 None:intergenic
GGAAAGGGTATAAGACTGTT+TGG 0.390575 8:-60739768 MsaT044473.1:CDS
GGAAAGCTGCTGCCGACAAA+TGG 0.392444 8:-60740187 MsaT044473.1:CDS
TCTCTTGTGGCAGCTACCCT+TGG 0.404436 8:+60739695 None:intergenic
GTCTTCCGACGCTTGATTGT+CGG 0.414985 8:+60740119 None:intergenic
GTCTGATCGGTCCACCGATC+CGG 0.438684 8:+60740007 None:intergenic
CCCGCTGCTGTAAGATCTGT+TGG 0.442824 8:-60740089 MsaT044473.1:CDS
CCCTCTCTTAAAATTCTCTT+GGG 0.443216 8:+60740157 None:intergenic
GAATTGCTTGCTTATCTTCA+GGG 0.444946 8:-60739903 MsaT044473.1:CDS
ATCATCATCCGTATCATGGC+TGG 0.456429 8:+60739821 None:intergenic
AACAGCCTTGAACTCATTGT+TGG 0.460694 8:+60739647 None:intergenic
CATTGTTGGCACCATCAATT+GGG 0.461669 8:+60739661 None:intergenic
GGGCGTGAAGACCCAATTGA+TGG 0.486085 8:-60739672 MsaT044473.1:CDS
TCGTTCCTTTGCCAAGGATA+CGG 0.487676 8:+60739850 None:intergenic
GATGAAATTAACCGTATCCT+TGG 0.490434 8:-60739861 MsaT044473.1:CDS
GAAAGCTGCTGCCGACAAAT+GGG 0.492919 8:-60740186 MsaT044473.1:CDS
ATCAATCATCTCATAAGTCT+TGG 0.495138 8:+60743944 None:intergenic
CATCATCATCATCCGTATCA+TGG 0.496633 8:+60739817 None:intergenic
TTGGGCGAATTCCCATTTGT+CGG 0.497343 8:+60740175 None:intergenic
TCTGGTGCTGCTGAAGATAT+GGG 0.499437 8:-60740041 MsaT044473.1:CDS
CCAACAGATCTTACAGCAGC+GGG 0.502479 8:+60740089 None:intergenic
GTACGTCGTGAATGCGGAAA+GGG 0.504721 8:-60739783 MsaT044473.1:CDS
GCTGTAAGATCTGTTGGAGT+TGG 0.513039 8:-60740083 MsaT044473.1:CDS
GAAGAAAATGTCTAAGAGGT+TGG 0.514254 8:-60743985 None:intergenic
GTTTGGCTGAAGGAAGAAGA+GGG 0.514660 8:-60739744 MsaT044473.1:CDS
TGAGTTCAAGGCTGTTGTCT+AGG 0.515335 8:-60739640 None:intergenic
CTTCACGAGTGCCAGTTCAC+TGG 0.517956 8:+60739938 None:intergenic
TAAGATCTGTTGGAGTTGGC+GGG 0.518928 8:-60740079 MsaT044473.1:CDS
GGGAATTCACCTTCGCACTC+TGG 0.523578 8:-60740059 MsaT044473.1:CDS
ACCCATTCGTTCCTTTGCCA+AGG 0.525178 8:+60739844 None:intergenic
GGTCTTCACGCCCTCTCTTG+TGG 0.525936 8:+60739682 None:intergenic
GTAAGATCTGTTGGAGTTGG+CGG 0.525989 8:-60740080 MsaT044473.1:CDS
ATCCTTGGCAAAGGAACGAA+TGG 0.527436 8:-60739846 MsaT044473.1:CDS
CTTCAGGGCGGTCTCAACAT+CGG 0.528747 8:-60739888 MsaT044473.1:CDS
ACTGTTTGGGGTTTGGCTGA+AGG 0.533700 8:-60739754 MsaT044473.1:CDS
AGGGTAGCTGCCACAAGAGA+GGG 0.548965 8:-60739692 MsaT044473.1:CDS
AGAAGAGGGGAAAGACAAGG+TGG 0.549839 8:-60739730 MsaT044473.1:CDS
GAATGGGTCCAGCCATGATA+CGG 0.552219 8:-60739829 MsaT044473.1:CDS
GGGATCAACTTCGACCGGAT+CGG 0.553641 8:-60740021 MsaT044473.1:CDS
GGAAGAAGAAAATGTCTAAG+AGG 0.554539 8:-60743989 None:intergenic
CTTGATTGTCGGAAGCAGCT+CGG 0.555478 8:+60740130 None:intergenic
TGGTGCCAACAATGAGTTCA+AGG 0.555667 8:-60739652 MsaT044473.1:CDS
TCCAACAGATCTTACAGCAG+CGG 0.556507 8:+60740088 None:intergenic
GTGATTTCTTGGGGTGAAAG+TGG 0.563498 8:-60743903 MsaT044473.1:CDS
AAGGGTAGCTGCCACAAGAG+AGG 0.563776 8:-60739693 MsaT044473.1:CDS
GGTACGTCGTGAATGCGGAA+AGG 0.565791 8:-60739784 MsaT044473.1:CDS
GATATGGGATCAACTTCGAC+CGG 0.569648 8:-60740026 MsaT044473.1:CDS
TCCTTGGCAAAGGAACGAAT+GGG 0.573236 8:-60739845 MsaT044473.1:CDS
GGAAGAAGAGGGGAAAGACA+AGG 0.573733 8:-60739733 MsaT044473.1:CDS
AAAGGGTATAAGACTGTTTG+GGG 0.582432 8:-60739766 MsaT044473.1:CDS
ATCAACTTCGACCGGATCGG+TGG 0.586784 8:-60740018 MsaT044473.1:CDS
ATTAACCGTATCCTTGGCAA+AGG 0.593236 8:-60739855 MsaT044473.1:CDS
GGTTTGGCTGAAGGAAGAAG+AGG 0.605675 8:-60739745 MsaT044473.1:CDS
TGCTTCCGACAATCAAGCGT+CGG 0.618599 8:-60740124 MsaT044473.1:CDS
GAAATCACATCATTTGTGAG+AGG 0.623568 8:+60743918 None:intergenic
GGTGGAGATTGACACTGCCA+AGG 0.626051 8:-60739712 MsaT044473.1:CDS
AACAGATCTTACAGCAGCGG+GGG 0.636718 8:+60740091 None:intergenic
ACAAATGATGTGATTTCTTG+GGG 0.640243 8:-60743912 MsaT044473.1:CDS
TCTCGATGGATAAGTCTGAT+CGG 0.645977 8:+60739994 None:intergenic
TCAGCAGCACCAGAGTGCGA+AGG 0.648598 8:+60740050 None:intergenic
AATGTGGTACGTCGTGAATG+CGG 0.667673 8:-60739789 MsaT044473.1:CDS
TTTGGCTGAAGGAAGAAGAG+GGG 0.668209 8:-60739743 MsaT044473.1:CDS
GTGGAGATTGACACTGCCAA+GGG 0.681976 8:-60739711 MsaT044473.1:CDS
CAACAGATCTTACAGCAGCG+GGG 0.700402 8:+60740090 None:intergenic
TCAGGGCGGTCTCAACATCG+GGG 0.720922 8:-60739886 MsaT044473.1:CDS
TTGCTTGCTTATCTTCAGGG+CGG 0.721162 8:-60739900 MsaT044473.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 60739641 60744000 60739641 ID=MsaG044473
Chr8 mRNA 60739641 60744000 60739641 ID=MsaT044473.1;Parent=MsaG044473
Chr8 exon 60739641 60740216 60739641 ID=MsaT044473.1.exon2;Parent=MsaT044473.1
Chr8 CDS 60739641 60740216 60739641 ID=cds.MsaT044473.1;Parent=MsaT044473.1
Chr8 exon 60743797 60744000 60743797 ID=MsaT044473.1.exon1;Parent=MsaT044473.1
Chr8 CDS 60743797 60744000 60743797 ID=cds.MsaT044473.1;Parent=MsaT044473.1
Gene Sequence

>MsaT044473.1

ATGTCTAAGAGGTTGGCAGCAGCACCATTTTTGACCAAGACTTATGAGATGATTGATGATCCTCTCACAAATGATGTGATTTCTTGGGGTGAAAGTGGCAACACTTTTGTTGTGGTGAAACAAGTTGAATTTTCAAGGGATTTGCTTCCAAAATATTTCAAGCACAACAATTTCTCAAGCTTTGTTCGTCAACTTAACACCTATGGGTTCCGGAAAGCTGCTGCCGACAAATGGGAATTCGCCCAAGAGAATTTTAAGAGAGGGGAGACCGAGCTGCTTCCGACAATCAAGCGTCGGAAGACCCAGTCCCCCGCTGCTGTAAGATCTGTTGGAGTTGGCGGGAATTCACCTTCGCACTCTGGTGCTGCTGAAGATATGGGATCAACTTCGACCGGATCGGTGGACCGATCAGACTTATCCATCGAGAACAAAAGACTCAAAATGGATAATGAAAAATTAACCAGTGAACTGGCACTCGTGAAGAAAAAGTGTGAAGAATTGCTTGCTTATCTTCAGGGCGGTCTCAACATCGGGGCTGATGAAATTAACCGTATCCTTGGCAAAGGAACGAATGGGTCCAGCCATGATACGGATGATGATGATGATGACAATGTGGTACGTCGTGAATGCGGAAAGGGTATAAGACTGTTTGGGGTTTGGCTGAAGGAAGAAGAGGGGAAAGACAAGGTGGAGATTGACACTGCCAAGGGTAGCTGCCACAAGAGAGGGCGTGAAGACCCAATTGATGGTGCCAACAATGAGTTCAAGGCTGTTGTCTAG

Protein sequence

>MsaT044473.1

MSKRLAAAPFLTKTYEMIDDPLTNDVISWGESGNTFVVVKQVEFSRDLLPKYFKHNNFSSFVRQLNTYGFRKAAADKWEFAQENFKRGETELLPTIKRRKTQSPAAVRSVGVGGNSPSHSGAAEDMGSTSTGSVDRSDLSIENKRLKMDNEKLTSELALVKKKCEELLAYLQGGLNIGADEINRILGKGTNGSSHDTDDDDDDNVVRRECGKGIRLFGVWLKEEEGKDKVEIDTAKGSCHKRGREDPIDGANNEFKAVV*