AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044565

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044565 MtrunA17_Chr4g0053901 98.427 445 5 1 1 443 25 469 0.0 912
MsaG044565 MtrunA17_Chr5g0401421 68.502 454 125 8 1 443 1 447 0.0 617
MsaG044565 MtrunA17_Chr2g0323111 46.569 204 90 1 240 443 123 307 1.06e-59 197
MsaG044565 MtrunA17_Chr2g0323111 49.451 91 40 1 28 118 45 129 1.02e-18 86.7
MsaG044565 MtrunA17_Chr5g0401131 29.469 414 251 8 35 443 114 491 3.04e-53 185
MsaG044565 MtrunA17_Chr2g0329591 29.508 427 237 12 34 443 73 452 1.07e-48 172
MsaG044565 MtrunA17_Chr3g0125811 27.358 424 264 7 19 441 189 569 5.02e-43 159
MsaG044565 MtrunA17_Chr1g0159461 27.073 410 257 7 33 441 223 591 6.21e-42 156
MsaG044565 MtrunA17_Chr5g0447691 27.163 416 251 9 32 441 174 543 4.17e-39 148
MsaG044565 MtrunA17_Chr4g0076091 27.381 420 248 10 32 442 172 543 1.46e-38 146
MsaG044565 MtrunA17_Chr8g0357601 27.119 413 250 12 36 441 183 551 8.78e-37 142
MsaG044565 MtrunA17_Chr5g0445331 25.728 412 266 8 32 441 158 531 1.85e-36 140
MsaG044565 MtrunA17_Chr3g0101141 24.703 421 270 9 23 441 166 541 7.47e-36 139
MsaG044565 MtrunA17_Chr2g0319091 25.240 416 259 7 32 441 209 578 5.17e-35 137
MsaG044565 MtrunA17_Chr8g0344101 28.276 435 240 14 28 441 216 599 5.88e-35 137
MsaG044565 MtrunA17_Chr7g0224271 26.316 418 252 9 35 441 300 672 1.12e-33 134
MsaG044565 MtrunA17_Chr2g0290241 25.728 412 263 9 32 441 54 424 5.20e-32 126
MsaG044565 MtrunA17_Chr2g0306641 25.412 425 244 14 34 444 124 489 1.70e-30 123
MsaG044565 MtrunA17_Chr4g0031711 23.608 449 291 11 3 444 332 735 2.16e-30 124
MsaG044565 MtrunA17_Chr3g0085681 24.091 440 270 10 36 441 26 435 2.21e-30 122
MsaG044565 MtrunA17_Chr3g0110971 25.296 423 243 10 36 441 220 586 3.05e-30 123
MsaG044565 MtrunA17_Chr2g0328391 23.558 416 273 10 32 443 296 670 4.43e-28 117
MsaG044565 MtrunA17_Chr4g0031731 23.923 418 268 13 32 442 251 625 1.06e-27 115
MsaG044565 MtrunA17_Chr4g0057891 26.168 428 240 14 34 441 148 519 1.06e-27 115
MsaG044565 MtrunA17_Chr4g0038851 24.124 485 286 13 5 441 18 468 3.09e-27 113
MsaG044565 MtrunA17_Chr8g0376381 24.757 412 244 12 36 441 170 521 3.30e-27 114
MsaG044565 MtrunA17_Chr4g0031721 24.762 420 263 12 32 444 313 686 3.76e-27 114
MsaG044565 MtrunA17_Chr5g0396491 23.881 469 286 10 15 441 38 477 4.94e-27 113
MsaG044565 MtrunA17_Chr4g0031741 25.783 415 255 12 35 443 284 651 5.98e-27 114
MsaG044565 MtrunA17_Chr7g0238891 23.923 418 269 9 32 444 369 742 6.97e-27 114
MsaG044565 MtrunA17_Chr4g0031701 23.741 417 270 12 32 442 251 625 7.77e-27 113
MsaG044565 MtrunA17_Chr2g0328381 23.611 432 279 10 17 443 257 642 4.06e-25 108
MsaG044565 MtrunA17_Chr2g0328341 23.684 418 268 10 32 444 269 640 9.91e-25 107
MsaG044565 MtrunA17_Chr6g0468561 26.190 420 252 10 31 441 291 661 1.17e-24 107
MsaG044565 MtrunA17_Chr3g0085361 24.576 354 213 9 120 441 18 349 1.36e-22 98.6
MsaG044565 MtrunA17_Chr2g0328411 22.628 411 270 8 35 442 289 654 1.71e-22 100
MsaG044565 MtrunA17_Chr4g0057291 21.770 418 274 11 35 444 292 664 1.26e-21 97.8
MsaG044565 MtrunA17_Chr4g0039661 25.882 425 244 16 30 441 188 554 1.77e-21 97.1
MsaG044565 MtrunA17_Chr2g0328431 21.739 414 274 8 35 444 289 656 2.42e-21 97.1
MsaG044565 MtrunA17_Chr1g0183641 24.578 415 244 15 35 441 123 476 6.78e-21 95.1
MsaG044565 MtrunA17_Chr7g0236851 24.451 319 199 5 128 441 375 656 8.91e-20 92.0
MsaG044565 MtrunA17_Chr7g0245601 23.301 412 251 14 38 443 441 793 6.58e-19 89.7
MsaG044565 MtrunA17_Chr2g0328401 22.513 382 247 8 35 413 290 625 1.45e-18 88.2
MsaG044565 MtrunA17_Chr4g0038761 22.927 410 259 14 38 443 87 443 3.12e-18 86.7
MsaG044565 MtrunA17_Chr1g0200401 23.918 439 242 17 35 441 80 458 1.98e-17 84.3
MsaG044565 MtrunA17_Chr2g0328361 20.714 420 253 10 32 444 271 617 3.69e-16 80.9
MsaG044565 MtrunA17_Chr5g0424131 24.941 421 261 16 34 441 120 498 8.26e-16 79.7
MsaG044565 MtrunA17_Chr8g0383981 22.912 419 253 14 35 443 139 497 1.40e-15 79.0
MsaG044565 MtrunA17_Chr3g0114841 23.515 421 269 14 34 441 119 499 1.45e-15 79.0
MsaG044565 MtrunA17_Chr4g0054071 34.211 114 66 3 33 144 131 237 1.35e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044565 AT3G49950.1 56.591 440 146 10 12 444 9 410 2.18e-171 487
MsaG044565 AT5G67411.1 55.947 227 73 4 159 383 1 202 9.31e-77 238
MsaG044565 AT4G37650.1 29.621 422 251 8 33 443 144 530 4.18e-52 184
MsaG044565 AT1G21450.1 25.791 411 262 7 33 441 223 592 3.21e-39 149
MsaG044565 AT2G04890.1 26.034 411 255 8 32 441 50 412 2.45e-38 144
MsaG044565 AT1G50600.3 25.412 425 268 10 20 441 147 525 1.16e-37 144
MsaG044565 AT1G50600.2 25.412 425 268 10 20 441 222 600 2.02e-37 144
MsaG044565 AT1G50600.1 25.412 425 268 10 20 441 218 596 2.19e-37 144
MsaG044565 AT5G48150.1 26.988 415 246 10 36 441 123 489 2.30e-37 142
MsaG044565 AT5G48150.4 26.988 415 246 10 36 441 123 489 2.30e-37 142
MsaG044565 AT5G48150.2 26.988 415 246 10 36 441 123 489 2.30e-37 142
MsaG044565 AT5G48150.3 26.988 415 246 10 36 441 123 489 2.30e-37 142
MsaG044565 AT4G17230.1 26.988 415 257 8 32 443 155 526 4.86e-34 134
MsaG044565 AT4G17230.2 26.988 415 257 8 32 443 155 526 4.86e-34 134
MsaG044565 AT1G50420.1 25.835 449 264 11 35 441 57 478 1.58e-31 126
MsaG044565 AT3G03450.1 25.362 414 248 9 36 441 184 544 2.17e-30 124
MsaG044565 AT5G17490.1 24.818 411 252 8 34 441 159 515 2.97e-30 123
MsaG044565 AT2G29065.1 26.316 418 261 11 35 444 252 630 8.09e-30 122
MsaG044565 AT3G46600.2 25.060 419 260 9 32 443 1 372 1.14e-28 116
MsaG044565 AT3G46600.1 25.000 420 261 9 32 444 209 581 5.35e-28 117
MsaG044565 AT5G52510.1 23.278 421 268 7 31 441 264 639 6.03e-28 117
MsaG044565 AT3G46600.3 25.060 419 260 9 32 443 177 548 6.42e-28 116
MsaG044565 AT2G29060.2 24.709 429 263 12 28 444 277 657 2.02e-26 112
MsaG044565 AT2G29060.1 24.709 429 263 12 28 444 314 694 2.27e-26 112
MsaG044565 AT1G07530.1 23.776 429 281 10 19 443 380 766 3.75e-26 112
MsaG044565 AT2G01570.1 24.146 410 253 12 36 441 225 580 7.23e-26 110
MsaG044565 AT3G13840.1 26.730 419 242 15 33 441 146 509 1.02e-25 109
MsaG044565 AT5G59450.1 23.490 447 290 10 6 444 198 600 3.68e-25 108
MsaG044565 AT1G14920.1 23.659 410 255 11 36 441 173 528 5.62e-24 104
MsaG044565 AT2G37650.2 23.317 416 270 11 32 443 332 702 8.26e-24 105
MsaG044565 AT2G37650.1 23.317 416 270 11 32 443 344 714 8.38e-24 105
MsaG044565 AT5G41920.1 25.542 415 243 15 35 441 43 399 4.98e-21 95.1
MsaG044565 AT1G55580.1 22.273 440 276 11 32 441 41 444 6.42e-20 92.0
MsaG044565 AT3G54220.1 24.213 413 249 15 38 443 296 651 7.38e-20 92.8
MsaG044565 AT1G63100.2 22.642 424 264 13 35 441 277 653 3.93e-19 90.5
MsaG044565 AT1G63100.1 22.642 424 264 13 35 441 252 628 6.12e-19 90.1
MsaG044565 AT5G66770.1 24.084 382 234 12 34 410 219 549 8.68e-19 89.4
MsaG044565 AT4G08250.1 24.942 429 243 20 34 441 106 476 4.21e-13 71.6
MsaG044565 AT3G50650.1 23.393 389 236 12 64 441 204 541 2.54e-11 66.2

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGGTTTGGTAGGCTTATC+TGG 0.192122 8:+62042940 None:intergenic
GATCTTTCTTACGACGAATT+AGG 0.234419 8:-62042370 MsaT044565.1:CDS
TAACACCTTGGCATCGTTTC+GGG 0.237807 8:-62042597 MsaT044565.1:CDS
CTTTGTTCCCATTTGCCTAT+AGG 0.258304 8:+62041827 None:intergenic
GGTCTGCATTTAATTATCTA+TGG 0.266633 8:-62041982 MsaT044565.1:CDS
GTTCAACGTTTGGTTTATTC+AGG 0.267502 8:-62042241 MsaT044565.1:CDS
CATATGAGTTTGAATTTGAT+AGG 0.282770 8:+62042164 None:intergenic
ACAACTTGGTTAGTAGATTA+AGG 0.285644 8:-62042003 MsaT044565.1:CDS
CTTCTTTGATTGAACATTTG+AGG 0.307298 8:-62042264 MsaT044565.1:CDS
TACTTGATTGTTCTTGTTCA+TGG 0.313905 8:+62042966 None:intergenic
CACTTGCTCAACAAATCCTT+TGG 0.324306 8:-62042819 MsaT044565.1:CDS
CACGATTTGTGAGGGCTCTA+AGG 0.339226 8:+62042737 None:intergenic
TCCAAAGCTTTAACTACCTT+TGG 0.348338 8:-62042907 MsaT044565.1:CDS
TTCTTTGATTGAACATTTGA+GGG 0.357439 8:-62042263 MsaT044565.1:CDS
TTAACACCTTGGCATCGTTT+CGG 0.361682 8:-62042598 MsaT044565.1:CDS
CTGTTACTACTACGTCTCTT+AGG 0.373908 8:-62042105 MsaT044565.1:CDS
TAGTTAAAGCTTTGGATGTT+GGG 0.379512 8:+62042914 None:intergenic
ATGAACATATTGTGCTTACT+TGG 0.379983 8:-62041697 MsaT044565.1:CDS
TGAGAATCTGTGTGTATCTA+TGG 0.382234 8:+62042648 None:intergenic
AGCTGCTAATGCAGCAATTC+TGG 0.384262 8:-62042569 MsaT044565.1:CDS
ACATTTGAGGGTTCAACGTT+TGG 0.395244 8:-62042251 MsaT044565.1:CDS
GGTTTATTCAGGTGAAGCTT+TGG 0.398229 8:-62042230 MsaT044565.1:CDS
TTGTTTCTTAACGCGCTTCG+AGG 0.401307 8:-62042079 MsaT044565.1:CDS
ACCAAAGGTAGTTAAAGCTT+TGG 0.401348 8:+62042906 None:intergenic
GAATTTGATAGGGTTTCATC+TGG 0.416336 8:+62042175 None:intergenic
AAATGTTGAACTAATTAAAC+AGG 0.426121 8:+62041638 None:intergenic
ACAAATCGTGCAGCGAAAAC+CGG 0.428781 8:-62042724 MsaT044565.1:CDS
GTAGTTAAAGCTTTGGATGT+TGG 0.435151 8:+62042913 None:intergenic
GGTATGAAGCAGATATTTGT+TGG 0.435178 8:-62041904 MsaT044565.1:CDS
ACTTGCTCAACAAATCCTTT+GGG 0.443862 8:-62042818 MsaT044565.1:CDS
GAGTTGAACCTATAGGCAAA+TGG 0.454475 8:-62041835 MsaT044565.1:CDS
TGATGAACATGCTGCTGGAT+GGG 0.466685 8:-62041735 MsaT044565.1:CDS
GTTGGGAACCATGGTTTGGT+AGG 0.471948 8:+62042931 None:intergenic
TCGTAAGAAAGATCAAGTTT+AGG 0.472515 8:+62042379 None:intergenic
GGATGTTGGGAACCATGGTT+TGG 0.473234 8:+62042927 None:intergenic
CATTGAGCTAGCTAACTTCG+TGG 0.473787 8:-62042623 MsaT044565.1:CDS
GATGAACATGCTGCTGGATG+GGG 0.481456 8:-62041734 MsaT044565.1:CDS
CGACGAATTAGGTTCTAAGT+TGG 0.484399 8:-62042359 MsaT044565.1:CDS
TGATTTAACTTCAAACAACT+TGG 0.488787 8:-62042017 MsaT044565.1:CDS
GTTGAGAGAGTTGAACCTAT+AGG 0.489058 8:-62041842 MsaT044565.1:CDS
GCGATAGCATCGATTAATGT+AGG 0.493202 8:+62042481 None:intergenic
TTGAGCAAGTGTTACATCGT+TGG 0.495134 8:+62042831 None:intergenic
GTTTCTTCAAGTTACACAAA+TGG 0.496382 8:-62042292 MsaT044565.1:CDS
AGTTGAACCTATAGGCAAAT+GGG 0.497812 8:-62041834 MsaT044565.1:CDS
TCTACTAAAATAACAAGTGT+TGG 0.502927 8:+62042049 None:intergenic
ATGCTTGATGAACATGCTGC+TGG 0.503248 8:-62041740 MsaT044565.1:CDS
CTAAGTTGGTGAATTTCGCT+AGG 0.512357 8:-62042345 MsaT044565.1:CDS
CATATTGTGCTTACTTGGAA+AGG 0.528509 8:-62041692 MsaT044565.1:CDS
ATATGAGTTTGAATTTGATA+GGG 0.529498 8:+62042165 None:intergenic
AATGTGTCCACAGTATCATA+AGG 0.533273 8:+62041956 None:intergenic
TTTCGCTGCACGATTTGTGA+GGG 0.537309 8:+62042729 None:intergenic
GCTTTGGATGTTGGGAACCA+TGG 0.537432 8:+62042922 None:intergenic
TTGATGAACATGCTGCTGGA+TGG 0.537625 8:-62041736 MsaT044565.1:CDS
TCAAGGGATTGGATTTGGTG+AGG 0.546032 8:-62041786 MsaT044565.1:CDS
TGATCAAAGGAGCAACTTCG+TGG 0.560682 8:+62042452 None:intergenic
AGTAGTAGCAGTAGTAGTAG+AGG 0.575130 8:+62042126 None:intergenic
TAAGAAAGATCAAGTTTAGG+TGG 0.577766 8:+62042382 None:intergenic
TGATTTGAGTCACCATCTTG+TGG 0.581981 8:+62042778 None:intergenic
GGATTTGGTGAGGAATGTGT+TGG 0.588738 8:-62041776 MsaT044565.1:CDS
CTTTGGTGACACAAACTGCA+TGG 0.596433 8:-62042890 MsaT044565.1:CDS
GCAATTCTGGAAGCAACAGA+AGG 0.597475 8:-62042556 MsaT044565.1:CDS
TGGTGAGGAATGTGTTGGTG+AGG 0.600886 8:-62041771 MsaT044565.1:CDS
CATGCAGTTTGTGTCACCAA+AGG 0.602334 8:+62042891 None:intergenic
TAGGTCAAGAAACGTGACAT+TGG 0.616837 8:-62042326 MsaT044565.1:CDS
ACTTCGTGGATTTAACACCT+TGG 0.617259 8:-62042609 MsaT044565.1:CDS
GAAACAGTGAGTTTGATCAA+AGG 0.618628 8:+62042439 None:intergenic
ATGGATACCTTATGATACTG+TGG 0.620359 8:-62041963 MsaT044565.1:CDS
AACAACATAGCACCACAAGA+TGG 0.622233 8:-62042790 MsaT044565.1:CDS
GCGTACCCGAAACGATGCCA+AGG 0.644547 8:+62042592 None:intergenic
GTGGAAGTAAACAAAGGCAA+TGG 0.648634 8:-62041925 MsaT044565.1:CDS
GGTTTGGTAGGCTTATCTGG+TGG 0.669809 8:+62042943 None:intergenic
CTATGTTGTTGAGAACCCAA+AGG 0.670186 8:+62042803 None:intergenic
TGCCTTTGTTTACTTCCACG+TGG 0.684183 8:+62041929 None:intergenic
GAGAATGTGATAGCTCATGA+AGG 0.690740 8:-62041875 MsaT044565.1:CDS
CAGATAAGCCTACCAAACCA+TGG 0.694388 8:-62042939 MsaT044565.1:CDS
TGCCACGTGGAAGTAAACAA+AGG 0.696360 8:-62041931 MsaT044565.1:CDS
GTGGACACATTTCTGCCACG+TGG 0.714542 8:-62041944 MsaT044565.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 62041652 62042986 62041652 ID=MsaG044565
Chr8 mRNA 62041652 62042986 62041652 ID=MsaT044565.1;Parent=MsaG044565
Chr8 exon 62041652 62042986 62041652 ID=MsaT044565.1.exon1;Parent=MsaT044565.1
Chr8 CDS 62041652 62042986 62041652 ID=cds.MsaT044565.1;Parent=MsaT044565.1
Gene Sequence

>MsaT044565.1

ATGAACAAGAACAATCAAGTACCACCAGATAAGCCTACCAAACCATGGTTCCCAACATCCAAAGCTTTAACTACCTTTGGTGACACAAACTGCATGGAACAGTTACTTGTTCATTGCGCAAACGCAATCGAAACCAACGATGTAACACTTGCTCAACAAATCCTTTGGGTTCTCAACAACATAGCACCACAAGATGGTGACTCAAATCAACGCTTAGCTTATAGTTTCCTTAGAGCCCTCACAAATCGTGCAGCGAAAACCGGTACCTGTAAAATGCTAGTAGAACAAGTTTATTCAAATGCTCATAACAATCTCACCATAGATACACACAGATTCTCAGTCATTGAGCTAGCTAACTTCGTGGATTTAACACCTTGGCATCGTTTCGGGTACGCAGCTGCTAATGCAGCAATTCTGGAAGCAACAGAAGGTTTCTCAGTGATTCATATAGTTGATTTGAGTTCAACTCATTGCATGCAGATTCCTACATTAATCGATGCTATCGCTAATCGCCACGAAGTTGCTCCTTTGATCAAACTCACTGTTTCTGATCATGCTAACAACAACAGTGATCAACTAATTCCACCTAAACTTGATCTTTCTTACGACGAATTAGGTTCTAAGTTGGTGAATTTCGCTAGGTCAAGAAACGTGACATTGGAATTTAGAGTAGTTTCTTCAAGTTACACAAATGGTTTTGCTTCTTTGATTGAACATTTGAGGGTTCAACGTTTGGTTTATTCAGGTGAAGCTTTGGTTATAAATTGTCACATGATGCTTCATTATATTCCAGATGAAACCCTATCAAATTCAAACTCATATGTTTATGATTCTATGTCCTCTACTACTACTGCTACTACTGTTACTACTACGTCTCTTAGGTCGTTGTTTCTTAACGCGCTTCGAGGTTTAGATCCAACACTTGTTATTTTAGTAGATGAAGATGTTGATTTAACTTCAAACAACTTGGTTAGTAGATTAAGGTCTGCATTTAATTATCTATGGATACCTTATGATACTGTGGACACATTTCTGCCACGTGGAAGTAAACAAAGGCAATGGTATGAAGCAGATATTTGTTGGAAGATTGAGAATGTGATAGCTCATGAAGGTGTTCAAAGAGTTGAGAGAGTTGAACCTATAGGCAAATGGGAACAAAGAATGAGAAATGCTAATTTTCAAGGGATTGGATTTGGTGAGGAATGTGTTGGTGAGGTGAAAGCTATGCTTGATGAACATGCTGCTGGATGGGGATTGAAGAAAGAAGATGAACATATTGTGCTTACTTGGAAAGGACATAATGTTGTTTTTGCTTCTGCTTGGTTACCTGTTTAA
Protein sequence

>MsaT044565.1

MNKNNQVPPDKPTKPWFPTSKALTTFGDTNCMEQLLVHCANAIETNDVTLAQQILWVLNNIAPQDGDSNQRLAYSFLRALTNRAAKTGTCKMLVEQVYSNAHNNLTIDTHRFSVIELANFVDLTPWHRFGYAAANAAILEATEGFSVIHIVDLSSTHCMQIPTLIDAIANRHEVAPLIKLTVSDHANNNSDQLIPPKLDLSYDELGSKLVNFARSRNVTLEFRVVSSSYTNGFASLIEHLRVQRLVYSGEALVINCHMMLHYIPDETLSNSNSYVYDSMSSTTTATTVTTTSLRSLFLNALRGLDPTLVILVDEDVDLTSNNLVSRLRSAFNYLWIPYDTVDTFLPRGSKQRQWYEADICWKIENVIAHEGVQRVERVEPIGKWEQRMRNANFQGIGFGEECVGEVKAMLDEHAAGWGLKKEDEHIVLTWKGHNVVFASAWLPV*