AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044566


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044566 MtrunA17_Chr4g0053911 98.283 233 4 0 1 233 1 233 2.11e-170 468
MsaG044566 MtrunA17_Chr5g0401401 71.008 238 57 5 1 233 1 231 2.18e-111 319
MsaG044566 MtrunA17_Chr7g0215281 61.417 127 46 1 1 124 3 129 5.47e-55 178
MsaG044566 MtrunA17_Chr6g0457431 55.319 141 51 1 1 141 3 131 9.50e-53 167
MsaG044566 MtrunA17_Chr1g0207471 63.566 129 43 1 1 129 1 125 2.89e-52 167
MsaG044566 MtrunA17_Chr6g0469271 60.550 109 41 1 1 107 3 111 1.54e-46 154
MsaG044566 MtrunA17_Chr6g0470651 51.667 120 54 3 1 118 3 120 8.73e-42 142
MsaG044566 MtrunA17_Chr6g0470631 49.194 124 59 3 1 122 3 124 1.13e-40 139
MsaG044566 MtrunA17_Chr1g0209841 50.000 120 46 3 1 118 3 110 1.73e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044566 AT4G37540.1 58.233 249 79 8 1 233 1 240 2.59e-83 248
MsaG044566 AT3G49940.1 57.540 252 81 7 1 232 1 246 1.30e-82 247
MsaG044566 AT5G67420.2 54.331 254 91 7 1 233 1 250 1.26e-75 229
MsaG044566 AT5G67420.1 54.331 254 91 7 1 233 1 250 1.26e-75 229
MsaG044566 AT1G67100.1 66.667 111 37 0 1 111 3 113 8.13e-54 173
MsaG044566 AT3G02550.1 49.718 177 73 4 1 173 3 167 3.80e-52 170
MsaG044566 AT1G68510.1 63.462 104 38 0 1 104 3 106 2.87e-48 159

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATTCGCGATCCGAATTT+CGG 0.249148 8:-62086818 MsaT044566.1:CDS
GTAGCGTGGCCTTGAGCTTC+TGG 0.259138 8:+62087268 None:intergenic
TTACCTGGTCTCTGTGGTTC+AGG 0.282554 8:+62087187 None:intergenic
ACCAGCTCGACCAAAGAATT+TGG 0.305659 8:+62087234 None:intergenic
GACTTCTTTGGACTGGAAAC+TGG 0.306937 8:-62086982 MsaT044566.1:CDS
ACGGTGCTGTTGGACTTCTT+TGG 0.315165 8:-62086994 MsaT044566.1:CDS
TTTATCGGAGCTGACACTTC+TGG 0.333923 8:-62086906 MsaT044566.1:CDS
AGATTCGCGATCCGAATTTC+GGG 0.347093 8:-62086817 MsaT044566.1:CDS
ACGGTGGCGCTGATGCTATT+TGG 0.350213 8:-62086841 MsaT044566.1:CDS
ACGACGGCGTGTTTAGAAAC+TGG 0.362464 8:-62086611 MsaT044566.1:CDS
TCTAGTAATAAGGTGTGTTC+TGG 0.372758 8:-62086779 MsaT044566.1:CDS
TGGCACGCTTAGGCCTTTAT+CGG 0.389750 8:-62086921 MsaT044566.1:CDS
GAGCTCGCCAGCCCGAAATT+CGG 0.420698 8:+62086806 None:intergenic
GAGCTCACGATCTAGTAATA+AGG 0.427686 8:-62086789 MsaT044566.1:CDS
GTCGATGACGTCGGAGGAAT+CGG 0.439790 8:-62086639 MsaT044566.1:CDS
AGAAGTGTCAGCTCCGATAA+AGG 0.449767 8:+62086908 None:intergenic
CAGAAAATCCTCATATGAAA+AGG 0.454062 8:+62086543 None:intergenic
TACCTGGTCTCTGTGGTTCA+GGG 0.463570 8:+62087188 None:intergenic
GCCAAATTCTTTGGTCGAGC+TGG 0.464513 8:-62087235 MsaT044566.1:CDS
GCTGTTGGACTTCTTTGGAC+TGG 0.482667 8:-62086989 MsaT044566.1:CDS
GACGTCATCGACGGTGAACC+AGG 0.485410 8:+62086650 None:intergenic
AATAGAAACATGAGTTGCAA+TGG 0.497518 8:-62087361 None:intergenic
GCAGTGGAGGAACGCCGGCC+TGG 0.500212 8:-62086668 MsaT044566.1:CDS
CGACGGCGTGTTTAGAAACT+GGG 0.509803 8:-62086610 MsaT044566.1:CDS
AGTCCAACAGCACCGTTCAC+AGG 0.514245 8:+62087001 None:intergenic
GGGTGCCGAGTTCTTCGAAA+AGG 0.515559 8:-62087340 MsaT044566.1:CDS
GTTTCGATCCATTGCAAGCA+AGG 0.521828 8:+62087292 None:intergenic
ATAGAAACATGAGTTGCAAT+GGG 0.529336 8:-62087360 None:intergenic
TTCAGGGACGTTAGAAATGA+AGG 0.532305 8:+62087204 None:intergenic
AAATCAAGATTAATCGCCGC+TGG 0.534004 8:+62086719 None:intergenic
GGTGGAGTCACGGTGGTGAT+CGG 0.543236 8:-62086577 MsaT044566.1:CDS
TAGAAACTGGGATTGGAGAT+CGG 0.545733 8:-62086598 MsaT044566.1:CDS
ATTGGAGATCGGTGGAGTCA+CGG 0.549508 8:-62086587 MsaT044566.1:CDS
AACCCTGTGAACGGTGCTGT+TGG 0.557626 8:-62087004 MsaT044566.1:CDS
GCGTGTTTAGAAACTGGGAT+TGG 0.557756 8:-62086605 MsaT044566.1:CDS
TTCTTCGCGGTGGCACGCTT+AGG 0.561098 8:-62086931 MsaT044566.1:CDS
GTTTGAAGCGTGTGGAAGAA+CGG 0.563405 8:-62087029 MsaT044566.1:CDS
TCGCGATCCGAATTTCGGGC+TGG 0.563771 8:-62086813 MsaT044566.1:CDS
TGGAAGCAAACGGAGGAGAT+CGG 0.569877 8:-62086759 MsaT044566.1:CDS
TGCCACTGATGAAGCTTCTG+AGG 0.578795 8:-62086870 MsaT044566.1:CDS
TCATCGACGGTGAACCAGGC+CGG 0.580860 8:+62086654 None:intergenic
AGGTGTGTTCTGGAAGCAAA+CGG 0.583068 8:-62086769 MsaT044566.1:CDS
AGAAGGCAGTGGAGGAACGC+CGG 0.585660 8:-62086673 MsaT044566.1:CDS
GAAGCTTCTGAGGCGGAAGA+CGG 0.587046 8:-62086860 MsaT044566.1:CDS
GTCCCTGAACCACAGAGACC+AGG 0.593241 8:-62087190 MsaT044566.1:intron
CACTGATGAAGCTTCTGAGG+CGG 0.596477 8:-62086867 MsaT044566.1:CDS
TCTCTACTGTTTGAAGCGTG+TGG 0.600037 8:-62087037 MsaT044566.1:CDS
GTCCAACAGCACCGTTCACA+GGG 0.600320 8:+62087002 None:intergenic
GCTTCATCAGTGGCAGTGAG+AGG 0.608069 8:+62086878 None:intergenic
CGCCTCAGAAGCTTCATCAG+TGG 0.613409 8:+62086868 None:intergenic
GAGATCGGAGGAGTTTGTGA+AGG 0.623682 8:-62086744 MsaT044566.1:CDS
GCGGTTGAGACAGTTCTTCG+CGG 0.626359 8:-62086944 MsaT044566.1:CDS
AAATAATTACCTGGTCTCTG+TGG 0.626571 8:+62087181 None:intergenic
GCTTCTGAGGCGGAAGACGG+TGG 0.641101 8:-62086857 MsaT044566.1:CDS
GATTCCTCCGACGTCATCGA+CGG 0.648497 8:+62086641 None:intergenic
TGGTTCACCGTCGATGACGT+CGG 0.655686 8:-62086648 MsaT044566.1:CDS
AAGCAAACGGAGGAGATCGG+AGG 0.657755 8:-62086756 MsaT044566.1:CDS
GGAGGAATCGGTGACGACGA+CGG 0.660492 8:-62086627 MsaT044566.1:CDS
CTGGCACGTGTGTCAAGCAG+CGG 0.661568 8:-62086963 MsaT044566.1:CDS
AGAACGGTTAACCCTGTGAA+CGG 0.663812 8:-62087013 MsaT044566.1:CDS
GGAGTTTGTGAAGGTTCCAG+CGG 0.666158 8:-62086735 MsaT044566.1:CDS
GTTGAGACAGTTCTTCGCGG+TGG 0.669818 8:-62086941 MsaT044566.1:CDS
ATCGAAACTCCAGAAGCTCA+AGG 0.686071 8:-62087277 MsaT044566.1:CDS
AAACTGGGATTGGAGATCGG+TGG 0.696279 8:-62086595 MsaT044566.1:CDS
TTCACCGTCGATGACGTCGG+AGG 0.699966 8:-62086645 MsaT044566.1:CDS
TGGCTACGAAAACAGTAGCG+TGG 0.700795 8:+62087254 None:intergenic
TGTGTTCTGGAAGCAAACGG+AGG 0.716901 8:-62086766 MsaT044566.1:CDS
GGAGATCGGTGGAGTCACGG+TGG 0.745894 8:-62086584 MsaT044566.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 62086553 62087374 62086553 ID=MsaG044566
Chr8 mRNA 62086553 62087374 62086553 ID=MsaT044566.1;Parent=MsaG044566
Chr8 exon 62086553 62087070 62086553 ID=MsaT044566.1.exon2;Parent=MsaT044566.1
Chr8 CDS 62086553 62087070 62086553 ID=cds.MsaT044566.1;Parent=MsaT044566.1
Chr8 exon 62087191 62087374 62087191 ID=MsaT044566.1.exon1;Parent=MsaT044566.1
Chr8 CDS 62087191 62087374 62087191 ID=cds.MsaT044566.1;Parent=MsaT044566.1
Gene Sequence

>MsaT044566.1

ATGAGTTGCAATGGGTGCCGAGTTCTTCGAAAAGGTTGCAGTGAGTCATGTATTTTACGACCTTGCTTGCAATGGATCGAAACTCCAGAAGCTCAAGGCCACGCTACTGTTTTCGTAGCCAAATTCTTTGGTCGAGCTGGTCTCATGTCCTTCATTTCTAACGTCCCTGAACCACAGAGACCAGCTCTGTTTCAGTCTCTACTGTTTGAAGCGTGTGGAAGAACGGTTAACCCTGTGAACGGTGCTGTTGGACTTCTTTGGACTGGAAACTGGCACGTGTGTCAAGCAGCGGTTGAGACAGTTCTTCGCGGTGGCACGCTTAGGCCTTTATCGGAGCTGACACTTCTGGACGCACCTCTCACTGCCACTGATGAAGCTTCTGAGGCGGAAGACGGTGGCGCTGATGCTATTTGGAAGATTCGCGATCCGAATTTCGGGCTGGCGAGCTCACGATCTAGTAATAAGGTGTGTTCTGGAAGCAAACGGAGGAGATCGGAGGAGTTTGTGAAGGTTCCAGCGGCGATTAATCTTGATTTACGATTGACGCCAATTTTTCAACAGAAGGCAGTGGAGGAACGCCGGCCTGGTTCACCGTCGATGACGTCGGAGGAATCGGTGACGACGACGGCGTGTTTAGAAACTGGGATTGGAGATCGGTGGAGTCACGGTGGTGATCGGAAAGTTCTCAACCTTTTCATATGA

Protein sequence

>MsaT044566.1

MSCNGCRVLRKGCSESCILRPCLQWIETPEAQGHATVFVAKFFGRAGLMSFISNVPEPQRPALFQSLLFEACGRTVNPVNGAVGLLWTGNWHVCQAAVETVLRGGTLRPLSELTLLDAPLTATDEASEAEDGGADAIWKIRDPNFGLASSRSSNKVCSGSKRRRSEEFVKVPAAINLDLRLTPIFQQKAVEERRPGSPSMTSEESVTTTACLETGIGDRWSHGGDRKVLNLFI*