AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044571


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044571 MtrunA17_Chr4g0053961 98.058 412 7 1 1 412 1 411 0.0 833
MsaG044571 MtrunA17_Chr5g0401351 71.078 408 107 4 5 412 7 403 0.0 609
MsaG044571 MtrunA17_Chr4g0001411 47.381 420 182 9 3 412 19 409 1.24e-124 366
MsaG044571 MtrunA17_Chr3g0105631 48.872 399 172 7 17 411 17 387 1.47e-120 355
MsaG044571 MtrunA17_Chr4g0068331 33.729 421 240 12 7 412 11 407 1.25e-62 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044571 AT2G23060.1 70.192 416 111 4 5 412 3 413 0.0 596
MsaG044571 AT4G37580.1 70.762 407 109 3 9 412 4 403 0.0 576
MsaG044571 AT4G37580.2 71.182 347 93 2 66 412 7 346 1.48e-175 494
MsaG044571 AT5G67430.1 62.162 407 127 8 6 412 7 386 4.19e-174 491
MsaG044571 AT2G23060.2 73.214 280 74 1 133 412 1 279 1.34e-152 433
MsaG044571 AT5G67430.2 62.573 342 106 7 70 411 1 320 8.95e-146 417
MsaG044571 AT2G30090.1 41.849 411 191 7 9 411 15 385 7.60e-99 300

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCGTTTGGTGTGAGTTTA+AGG 0.156297 8:-62163809 None:intergenic
CATAATCTTGCTATGGAAAA+TGG 0.159585 8:+62164251 MsaT044571.1:CDS
ACACTTTCTCTATCTTTATT+AGG 0.193995 8:-62163161 None:intergenic
ATTCCCATTCTTCTATGATT+TGG 0.243356 8:-62163582 None:intergenic
GTTCCGAGAGACGGAAAATA+TGG 0.259285 8:+62163933 MsaT044571.1:CDS
TGTGGTGTATTAAGAGATTA+GGG 0.277467 8:+62164363 MsaT044571.1:CDS
TTCACCATCTTCAACACTTC+TGG 0.289161 8:-62164209 None:intergenic
GAGTTACGGACTCTGCAAAT+AGG 0.309584 8:-62163257 None:intergenic
TTGTGGTGTATTAAGAGATT+AGG 0.337855 8:+62164362 MsaT044571.1:CDS
GTGTTTACCCTCGAAGTAAA+AGG 0.339022 8:+62164047 MsaT044571.1:CDS
TTTGGATTTCATCTAATGTA+TGG 0.345767 8:+62164170 MsaT044571.1:CDS
CGTGGGCTTTAGTAAGTGTT+TGG 0.349245 8:+62164012 MsaT044571.1:CDS
GAACAATGGTTTCGTGAAAA+TGG 0.352645 8:+62163630 MsaT044571.1:CDS
TAGGCTAGTTTAGTGAAAAC+AGG 0.396155 8:-62163537 None:intergenic
TTGAGACGGTGTTTGAAAAC+GGG 0.405778 8:-62163762 None:intergenic
AAACTTAATTAAAACTCTCT+AGG 0.411336 8:-62164455 None:intergenic
ATAATCTTGCTATGGAAAAT+GGG 0.419756 8:+62164252 MsaT044571.1:CDS
AATGGGAATAGGACTAAAAC+TGG 0.424053 8:+62163596 MsaT044571.1:CDS
TATAGCAATTCAGCGTCGTT+TGG 0.426241 8:-62163822 None:intergenic
AATCATAGAAGAATGGGAAT+AGG 0.430636 8:+62163585 MsaT044571.1:CDS
TGTCTTGTGAAGAAGATTTG+TGG 0.432512 8:+62164345 MsaT044571.1:CDS
CTCTCCAAATCATAGAAGAA+TGG 0.438768 8:+62163578 MsaT044571.1:CDS
AGTTACGGACTCTGCAAATA+GGG 0.441613 8:-62163256 None:intergenic
GTCTCTCGGAACGGCTAAGA+AGG 0.443084 8:-62163922 None:intergenic
GTGATGGTTCTGTTGGTGAT+TGG 0.444763 8:+62164396 MsaT044571.1:CDS
ATGGGGCCGGATCAAATAAC+TGG 0.446614 8:+62163952 MsaT044571.1:CDS
CTTACCAGCATGAGAAAAGT+TGG 0.450426 8:-62163281 None:intergenic
TCTCCAAATCATAGAAGAAT+GGG 0.450649 8:+62163579 MsaT044571.1:CDS
TTTCATCTAATGTATGGAAT+TGG 0.454495 8:+62164176 MsaT044571.1:CDS
AGTGAAAACAGGGACATGAT+TGG 0.457232 8:-62163526 None:intergenic
GCCGGACCAGTTATTTGATC+CGG 0.460530 8:-62163958 None:intergenic
TTTGAGACGGTGTTTGAAAA+CGG 0.461617 8:-62163763 None:intergenic
TGAGAAAAGTTGGTGAGTTA+CGG 0.469288 8:-62163271 None:intergenic
TGATGGACCCACCGAGTTCG+TGG 0.472987 8:+62163994 MsaT044571.1:CDS
TTCTTAGCCGTTCCGAGAGA+CGG 0.475720 8:+62163924 MsaT044571.1:CDS
CTTTGAGTAACCACATTTGT+CGG 0.477857 8:-62163712 None:intergenic
GAGTTTAAGGATTGTTACTT+TGG 0.478195 8:-62163796 None:intergenic
TTCCGAGAGACGGAAAATAT+GGG 0.480561 8:+62163934 MsaT044571.1:CDS
GCCGGATCAAATAACTGGTC+CGG 0.494724 8:+62163957 MsaT044571.1:CDS
CATCTAATGTATGGAATTGG+TGG 0.508655 8:+62164179 MsaT044571.1:CDS
AACTTAATTAAAACTCTCTA+GGG 0.509353 8:-62164454 None:intergenic
GAAGAGGGAAAGTTTACCAC+TGG 0.510294 8:-62163216 None:intergenic
TAACAAGGATTGACGGTGTA+CGG 0.511747 8:-62163737 None:intergenic
TACATTAGATGAAATCCAAA+CGG 0.515685 8:-62164167 None:intergenic
ATCTAATGTATGGAATTGGT+GGG 0.530410 8:+62164180 MsaT044571.1:CDS
GTTTGAAAACGGGGTTAACA+AGG 0.535510 8:-62163752 None:intergenic
GATTGGACTAAATCCAAACC+TGG 0.541245 8:+62164413 MsaT044571.1:CDS
GATGGACCCACCGAGTTCGT+GGG 0.542101 8:+62163995 MsaT044571.1:CDS
TCCCTCTTCACCGACTTGCA+CGG 0.552369 8:+62163230 MsaT044571.1:CDS
GATTATAGTGATGGTTCTGT+TGG 0.554056 8:+62164389 MsaT044571.1:CDS
TTGCTAACACACGTCTCACG+CGG 0.558176 8:-62164076 None:intergenic
TTAGGGGAAGATTATAGTGA+TGG 0.558836 8:+62164380 MsaT044571.1:CDS
ATGTATGGAATTGGTGGGGA+AGG 0.560905 8:+62164185 MsaT044571.1:CDS
AGGACCAGAAGTGTTGAAGA+TGG 0.565220 8:+62164205 MsaT044571.1:CDS
GAGATAGGCAATGAGACAGT+CGG 0.571965 8:+62163414 MsaT044571.1:CDS
CACCGTGCAAGTCGGTGAAG+AGG 0.582711 8:-62163232 None:intergenic
TGATAGACAAGGCTTTACCT+TGG 0.583323 8:+62164111 MsaT044571.1:CDS
CTTACTAAAGCCCACGAACT+CGG 0.584602 8:-62164005 None:intergenic
ACCGTGCAAGTCGGTGAAGA+GGG 0.587032 8:-62163231 None:intergenic
GACTCTTAAGCCTAAAACGT+AGG 0.591943 8:-62163556 None:intergenic
AGAGACGGAAAATATGGGGC+CGG 0.595183 8:+62163939 MsaT044571.1:CDS
TGGTGCTGAGTATTCATACA+TGG 0.597000 8:+62163650 MsaT044571.1:CDS
ACGGGGTTAACAAGGATTGA+CGG 0.599326 8:-62163744 None:intergenic
TGCTAACACACGTCTCACGC+GGG 0.608765 8:-62164075 None:intergenic
CTAAAGCCCACGAACTCGGT+GGG 0.630773 8:-62164001 None:intergenic
ATATGTGAAGTTGGACCCAG+TGG 0.635969 8:+62163200 MsaT044571.1:CDS
AACAACTCGCGTGATAGACA+AGG 0.641023 8:+62164100 MsaT044571.1:CDS
TCTAATGTATGGAATTGGTG+GGG 0.644769 8:+62164181 MsaT044571.1:CDS
TGAGACGGTGTTTGAAAACG+GGG 0.645003 8:-62163761 None:intergenic
AAGAGGGAAAGTTTACCACT+GGG 0.649201 8:-62163215 None:intergenic
TCCGAGAGACGGAAAATATG+GGG 0.649828 8:+62163935 MsaT044571.1:CDS
ACTAAAGCCCACGAACTCGG+TGG 0.653999 8:-62164002 None:intergenic
AAGATGGTGAAAGCTTTGTG+TGG 0.659803 8:+62164221 MsaT044571.1:CDS
AATAGGGTCACCGTGCAAGT+CGG 0.663384 8:-62163240 None:intergenic
CAAGACAAAACCTTCCAATG+AGG 0.667319 8:-62164329 None:intergenic
CAGTGGTGGAGAATTTGTAA+CGG 0.668778 8:-62163845 None:intergenic
TGAGGAATAGCAAAACGTAA+CGG 0.672089 8:-62164311 None:intergenic
GGAAAATGGGTGTAGTGCTG+TGG 0.676685 8:+62164265 MsaT044571.1:CDS
AGGCTAGTTTAGTGAAAACA+GGG 0.690252 8:-62163536 None:intergenic
ATTGGACTAAATCCAAACCT+GGG 0.691102 8:+62164414 MsaT044571.1:CDS
TGTATCAAAACCGTTACATG+CGG 0.691594 8:+62163450 MsaT044571.1:CDS
AAAACAGAATCAATGTCACG+AGG 0.691646 8:-62163876 None:intergenic
GTGGTGTATTAAGAGATTAG+GGG 0.708222 8:+62164364 MsaT044571.1:CDS
GAGACAGTCGGAATGATACG+AGG 0.713167 8:+62163426 MsaT044571.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 62163122 62164469 62163122 ID=MsaG044571
Chr8 mRNA 62163122 62164469 62163122 ID=MsaT044571.1;Parent=MsaG044571
Chr8 exon 62163122 62163298 62163122 ID=MsaT044571.1.exon1;Parent=MsaT044571.1
Chr8 CDS 62163122 62163298 62163122 ID=cds.MsaT044571.1;Parent=MsaT044571.1
Chr8 exon 62163408 62164469 62163408 ID=MsaT044571.1.exon2;Parent=MsaT044571.1
Chr8 CDS 62163408 62164469 62163408 ID=cds.MsaT044571.1;Parent=MsaT044571.1
Gene Sequence

>MsaT044571.1

ATGGGTGAAGTAGTAGTTGTGAATGTGAGAGAGTTTGATCCTAATAAAGATAGAGAAAGTGTTGAAGCAGTTGAAAAAATATGTGAAGTTGGACCCAGTGGTAAACTTTCCCTCTTCACCGACTTGCACGGTGACCCTATTTGCAGAGTCCGTAACTCACCAACTTTTCTCATGCTGGTAGCAGAGATAGGCAATGAGACAGTCGGAATGATACGAGGTTGTATCAAAACCGTTACATGCGGCAAAAAACTAACAAGACCAACAAAAAACACCACCACAGAAACAAATCAAAATTCCAATCATGTCCCTGTTTTCACTAAACTAGCCTACGTTTTAGGCTTAAGAGTCTCTCCAAATCATAGAAGAATGGGAATAGGACTAAAACTGGTAGAAAAAATGGAACAATGGTTTCGTGAAAATGGTGCTGAGTATTCATACATGGCTACCGAAAACGACAACGTTGCATCAGTTAAACTTTTCACCGACAAATGTGGTTACTCAAAGTTCCGTACACCGTCAATCCTTGTTAACCCCGTTTTCAAACACCGTCTCAAAACATCATCGTCCAAAGTAACAATCCTTAAACTCACACCAAACGACGCTGAATTGCTATACCGTTACAAATTCTCCACCACTGAGTTTTTTCCTCGTGACATTGATTCTGTTTTAAAAAACAAACTTACTCTTGGAACCTTCTTAGCCGTTCCGAGAGACGGAAAATATGGGGCCGGATCAAATAACTGGTCCGGCTCCGAAAGCTTTTTGATGGACCCACCGAGTTCGTGGGCTTTAGTAAGTGTTTGGAACTGCAAAGACGTGTTTACCCTCGAAGTAAAAGGCGCGTCCCGCGTGAGACGTGTGTTAGCAAAAACAACTCGCGTGATAGACAAGGCTTTACCTTGGTTAAAATTACCTTCGATTCCGAATTTTTTCAAACCGTTTGGATTTCATCTAATGTATGGAATTGGTGGGGAAGGACCAGAAGTGTTGAAGATGGTGAAAGCTTTGTGTGGTTTTGCTCATAATCTTGCTATGGAAAATGGGTGTAGTGCTGTGGCTACTGAAGTTTCTAGCTGTGAACCGTTACGTTTTGCTATTCCTCATTGGAAGGTTTTGTCTTGTGAAGAAGATTTGTGGTGTATTAAGAGATTAGGGGAAGATTATAGTGATGGTTCTGTTGGTGATTGGACTAAATCCAAACCTGGGTTCTCCATTTTTGTTGACCCTAGAGAGTTTTAA

Protein sequence

>MsaT044571.1

MGEVVVVNVREFDPNKDRESVEAVEKICEVGPSGKLSLFTDLHGDPICRVRNSPTFLMLVAEIGNETVGMIRGCIKTVTCGKKLTRPTKNTTTETNQNSNHVPVFTKLAYVLGLRVSPNHRRMGIGLKLVEKMEQWFRENGAEYSYMATENDNVASVKLFTDKCGYSKFRTPSILVNPVFKHRLKTSSSKVTILKLTPNDAELLYRYKFSTTEFFPRDIDSVLKNKLTLGTFLAVPRDGKYGAGSNNWSGSESFLMDPPSSWALVSVWNCKDVFTLEVKGASRVRRVLAKTTRVIDKALPWLKLPSIPNFFKPFGFHLMYGIGGEGPEVLKMVKALCGFAHNLAMENGCSAVATEVSSCEPLRFAIPHWKVLSCEEDLWCIKRLGEDYSDGSVGDWTKSKPGFSIFVDPREF*