AlfalfaGEDB | MsaG044585

M. sativa ssp. caerulea / MsaG044585

KEGG: K09422
GO: GO:0000228 GO:0000723 GO:0000781 GO:0000784 GO:0000785 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003697 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006139 GO:0006259 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009751 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010468 GO:0010556 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031627 GO:0031974 GO:0031981 GO:0032200 GO:0033993 GO:0034641 GO:0042162 GO:0042221 GO:0042493 GO:0042592 GO:0042802 GO:0042803 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046677 GO:0046686 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097305 GO:0098687 GO:0098847 GO:0140110 GO:1901360 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044585	XP_013457503.1	98.058	309	5	1	1	308	1	309	0.0	606

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044585	sp\|Q9FJW5\|TRB2_ARATH	50.000	310	142	4	1	308	1	299	7.43e-71	224

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044585	tr\|A0A072UNW9\|A0A072UNW9_MEDTR	98.058	309	5	1	1	308	1	309	0.0	606

TFs/TRs:

Gene ID	Type	Classification
MsaG044585	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG005839	MsaG044585	0.823998	1.812132e-53	4.435537e-51
MsaG006331	MsaG044585	0.800849	1.988755e-48	2.740934e-46
MsaG010053	MsaG044585	0.800651	2.182257e-48	2.994308e-46
MsaG012422	MsaG044585	0.806685	1.237496e-49	1.951835e-47
MsaG044585	MsaG045125	0.804647	3.298273e-49	4.959530e-47
MsaG044585	MsaG045446	0.801368	1.559317e-48	2.174458e-46
MsaG044585	MsaG046423	0.806862	1.135882e-49	1.798985e-47
MsaG044585	MsaG008796	0.802707	8.298967e-49	1.193128e-46
MsaG014014	MsaG044585	0.822031	5.181818e-53	1.202717e-50
MsaG014421	MsaG044585	0.843515	2.493779e-58	1.088712e-55
MsaG014422	MsaG044585	0.822467	4.109859e-53	9.651356e-51
MsaG021016	MsaG044585	0.801557	1.426793e-48	1.998186e-46
MsaG021896	MsaG044585	0.813890	3.511543e-51	6.601465e-49
MsaG028375	MsaG044585	0.800525	2.314331e-48	3.166413e-46
MsaG029899	MsaG044585	0.820635	1.083980e-52	2.424425e-50
MsaG030801	MsaG044585	0.805052	2.717515e-49	4.124971e-47
MsaG039739	MsaG044585	0.801259	1.640982e-48	2.282648e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044585	MtrunA17_Chr4g0054131	98.058	309	5	1	1	308	1	309	0.0	606
MsaG044585	MtrunA17_Chr5g0401061	66.346	312	83	7	1	308	1	294	8.75e-106	310
MsaG044585	MtrunA17_Chr2g0293381	45.276	254	120	6	1	250	1	239	3.37e-66	209
MsaG044585	MtrunA17_Chr8g0384671	44.531	256	117	6	1	250	1	237	5.65e-64	204
MsaG044585	MtrunA17_Chr8g0342871	36.585	287	142	8	1	280	1	254	4.74e-33	123
MsaG044585	MtrunA17_Chr1g0166681	69.048	84	25	1	1	84	1	83	8.26e-33	120
MsaG044585	MtrunA17_Chr2g0329921	38.785	214	114	5	1	201	1	210	3.36e-32	121
MsaG044585	MtrunA17_Chr8g0383821	48.305	118	57	3	1	114	1	118	2.56e-29	108
MsaG044585	MtrunA17_Chr1g0195261	70.000	50	15	0	1	50	1	50	5.36e-17	73.6
MsaG044585	MtrunA17_Chr1g0195251	37.398	123	67	5	136	255	296	411	2.54e-13	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044585	AT5G67580.2	50.000	310	142	4	1	308	1	299	7.55e-72	224
MsaG044585	AT5G67580.1	50.000	310	142	4	1	308	1	299	7.55e-72	224
MsaG044585	AT3G49850.1	47.386	306	143	6	1	304	1	290	2.14e-65	207
MsaG044585	AT3G49850.2	47.386	306	143	6	1	304	1	290	2.14e-65	207
MsaG044585	AT1G49950.3	42.188	256	128	7	1	252	1	240	4.91e-61	196
MsaG044585	AT1G49950.1	42.188	256	128	7	1	252	1	240	4.91e-61	196
MsaG044585	AT1G49950.2	42.188	256	128	7	1	252	1	240	4.91e-61	196
MsaG044585	AT1G72740.2	31.959	291	145	7	1	280	1	249	1.24e-30	117
MsaG044585	AT1G72740.1	31.773	299	141	9	1	280	1	255	2.27e-28	111
MsaG044585	AT1G17520.4	31.802	283	165	6	1	280	1	258	5.51e-28	110
MsaG044585	AT1G17520.1	32.155	283	170	7	1	280	1	264	3.86e-27	108
MsaG044585	AT1G17520.3	32.042	284	164	7	1	280	1	259	1.15e-26	106
MsaG044585	AT1G17520.2	32.394	284	169	8	1	280	1	265	8.80e-26	104

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTAAGCCCCTGACAATTTC+TGG	0.156688	8:-62319235	MsaT044585.1:CDS
AATGCAAAATGCTTCATATT+TGG	0.195627	8:-62317007	MsaT044585.1:CDS
TTGATAGCTCTGCCTCAATT+TGG	0.196523	8:+62317198	None:intergenic
CAATTGGTTATTGTCAGTTT+TGG	0.201241	8:+62319297	None:intergenic
ATTCGAACGCGTACGCAAAA+TGG	0.224697	8:+62319536	None:intergenic
GACAATAACTTTCTAAGATT+TGG	0.247003	8:+62318091	None:intergenic
CTCCACCTCCGGAGAATCTT+TGG	0.268223	8:+62317253	None:intergenic
TGTATTGTGAACAGGATAAA+TGG	0.295045	8:-62319393	MsaT044585.1:intron
TGATAGCTCTGCCTCAATTT+GGG	0.303115	8:+62317199	None:intergenic
ATGTTCCACTACAAATAGTT+AGG	0.338025	8:+62319188	None:intergenic
TCAAACACGGGGCTGGAAAA+TGG	0.376865	8:-62319590	MsaT044585.1:CDS
TCTGCAGTCCATTTCTGCTT+AGG	0.379164	8:+62319642	None:intergenic
CTACTTTACTCAAGGCCAAT+TGG	0.386042	8:+62319281	None:intergenic
ATATAAATGTCACAGCAATA+TGG	0.388272	8:-62319366	MsaT044585.1:CDS
CGCCTCCCTAGCTGCTGCTT+CGG	0.390489	8:+62317094	None:intergenic
ATGTTCCACCAGAAATTGTC+AGG	0.418565	8:+62319227	None:intergenic
TTCTCAGAGACCGCTGAACT+TGG	0.424839	8:+62317314	None:intergenic
GAAGAAGCGGCGTTGAAAGC+TGG	0.427269	8:-62319618	MsaT044585.1:CDS
TGGGTGCTCCTAAGCAGAAA+TGG	0.427716	8:-62319650	MsaT044585.1:CDS
TGCTGCTTCGGCCTGTGCAA+TGG	0.434469	8:+62317106	None:intergenic
TATAAATGTCACAGCAATAT+GGG	0.435123	8:-62319365	MsaT044585.1:CDS
GGGAGACAATAATGTATTAG+AGG	0.441902	8:-62318493	MsaT044585.1:CDS
AATAACTTTCTAAGATTTGG+TGG	0.446528	8:+62318094	None:intergenic
GCAGCCTTGTCAGAACCCTT+TGG	0.463786	8:+62318450	None:intergenic
AGCTGCAAAAGCAGTTGCAG+AGG	0.469233	8:-62317138	MsaT044585.1:CDS
CGAAGCAGCAGCTAGGGAGG+CGG	0.477225	8:-62317093	MsaT044585.1:CDS
CAAAACTGACAATAACCAAT+TGG	0.479938	8:-62319296	MsaT044585.1:CDS
GGAGTAGTCAAACACGGGGC+TGG	0.481269	8:-62319597	MsaT044585.1:CDS
CTTTGCAAAAGCAGCGATGA+AGG	0.481859	8:-62317036	MsaT044585.1:CDS
GTCCAAAGATTCTCCGGAGG+TGG	0.481885	8:-62317255	MsaT044585.1:CDS
ACAGGCCGAAGCAGCAGCTA+GGG	0.483254	8:-62317099	MsaT044585.1:CDS
GGCCGAAGCAGCAGCTAGGG+AGG	0.499766	8:-62317096	MsaT044585.1:CDS
TGTTCCACTACAAATAGTTA+GGG	0.502655	8:+62319189	None:intergenic
AGCGAGGTCCAAAGATTCTC+CGG	0.502665	8:-62317261	MsaT044585.1:CDS
TCACACATCTTCTCCACCTC+CGG	0.506821	8:+62317242	None:intergenic
CACAGGCCGAAGCAGCAGCT+AGG	0.511915	8:-62317100	MsaT044585.1:CDS
TGCCATTGCTTCTTACATAG+AGG	0.516855	8:-62318430	MsaT044585.1:intron
GTCAATATGAAGGAGCCAAA+GGG	0.526790	8:-62318465	MsaT044585.1:CDS
GTTCAAAAGAACAAGTATCA+AGG	0.540828	8:-62319153	MsaT044585.1:intron
CCTTTCAAAATCCCAAATTG+AGG	0.542802	8:-62317210	MsaT044585.1:CDS
TACCTCTATGTAAGAAGCAA+TGG	0.545469	8:+62318428	None:intergenic
TCACAGCAATATGGGGATCC+CGG	0.546612	8:-62319357	MsaT044585.1:CDS
GGCAGAAGTTGCCATTGCAC+AGG	0.551573	8:-62317117	MsaT044585.1:CDS
CGCAAAATGGAACTGAACTC+AGG	0.555443	8:+62319549	None:intergenic
AAGCTCTTCTCTGATGCTTG+TGG	0.556765	8:-62317285	MsaT044585.1:CDS
TTTCTGCTTAGGAGCACCCA+TGG	0.559262	8:+62319653	None:intergenic
AGAGGCTATTGTCAATATGA+AGG	0.562785	8:-62318475	MsaT044585.1:CDS
CTCTGATGCTTGTGGAAGCG+AGG	0.566860	8:-62317277	MsaT044585.1:CDS
TGTCAATATGAAGGAGCCAA+AGG	0.567981	8:-62318466	MsaT044585.1:CDS
GTCAGCAAAATTGCAGCAGA+TGG	0.568574	8:-62318071	MsaT044585.1:CDS
GACAAGTGGCAAAATAGTCC+AGG	0.570447	8:-62318047	MsaT044585.1:intron
AAAATGGCGCACAATACTCA+TGG	0.571706	8:-62319574	MsaT044585.1:CDS
TCAGCGGTCTCTGAGAAAAG+AGG	0.571845	8:-62317308	MsaT044585.1:CDS
ACGTTGAACTACTTTACTCA+AGG	0.573317	8:+62319273	None:intergenic
CTACAAATAGTTAGGGGCGT+AGG	0.586074	8:+62319196	None:intergenic
GGCAGAGCTATCAAAAGTGA+GGG	0.588740	8:-62317189	MsaT044585.1:CDS
CAAAATTATGCCAAGTTCAG+CGG	0.601681	8:-62317324	MsaT044585.1:CDS
GCGTTCGAATGTAGATCTCA+AGG	0.619214	8:-62319523	MsaT044585.1:intron
AAGCTGGAGTAGTCAAACAC+GGG	0.628346	8:-62319602	MsaT044585.1:CDS
AGGCAGAGCTATCAAAAGTG+AGG	0.628730	8:-62317190	MsaT044585.1:CDS
ACGCCCCTAACTATTTGTAG+TGG	0.636478	8:-62319193	MsaT044585.1:CDS
GATTGCAGAAGCTGAAGCTG+AGG	0.636628	8:-62317069	MsaT044585.1:CDS
GGGGATGTCAGCTCAAGAGG+CGG	0.637055	8:-62317168	MsaT044585.1:CDS
AAGCCCCTGACAATTTCTGG+TGG	0.637720	8:-62319232	MsaT044585.1:CDS
GGAGCCAAAGGGTTCTGACA+AGG	0.637821	8:-62318454	MsaT044585.1:CDS
TTGCAGCAGATGGTGACAAG+TGG	0.648634	8:-62318061	MsaT044585.1:CDS
ATAAATGTCACAGCAATATG+GGG	0.648999	8:-62319364	MsaT044585.1:CDS
TGTTCCACCAGAAATTGTCA+GGG	0.649357	8:+62319228	None:intergenic
ATGGACTGCAGAAGAAGAAG+CGG	0.652524	8:-62319631	MsaT044585.1:CDS
ATTGTGAACAGGATAAATGG+AGG	0.664041	8:-62319390	MsaT044585.1:intron
CAGAGCTATCAAAAGTGAGG+GGG	0.676061	8:-62317187	MsaT044585.1:CDS
AAAGCTGGAGTAGTCAAACA+CGG	0.681254	8:-62319603	MsaT044585.1:CDS
GCAGAGCTATCAAAAGTGAG+GGG	0.684532	8:-62317188	MsaT044585.1:CDS
GTTCCACCAGAAATTGTCAG+GGG	0.692592	8:+62319229	None:intergenic
GAGGTCCAAAGATTCTCCGG+AGG	0.723417	8:-62317258	MsaT044585.1:CDS
GTTCCACTACAAATAGTTAG+GGG	0.726078	8:+62319190	None:intergenic
AGCTGGAGTAGTCAAACACG+GGG	0.727088	8:-62319601	MsaT044585.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	62317004	62319673	62317004	ID=MsaG044585
Chr8	mRNA	62317004	62319673	62317004	ID=MsaT044585.1;Parent=MsaG044585
Chr8	exon	62317004	62317360	62317004	ID=MsaT044585.1.exon5;Parent=MsaT044585.1
Chr8	CDS	62317004	62317360	62317004	ID=cds.MsaT044585.1;Parent=MsaT044585.1
Chr8	exon	62318048	62318131	62318048	ID=MsaT044585.1.exon4;Parent=MsaT044585.1
Chr8	CDS	62318048	62318131	62318048	ID=cds.MsaT044585.1;Parent=MsaT044585.1
Chr8	exon	62318431	62318518	62318431	ID=MsaT044585.1.exon3;Parent=MsaT044585.1
Chr8	CDS	62318431	62318518	62318431	ID=cds.MsaT044585.1;Parent=MsaT044585.1
Chr8	exon	62319154	62319401	62319154	ID=MsaT044585.1.exon2;Parent=MsaT044585.1
Chr8	CDS	62319154	62319401	62319154	ID=cds.MsaT044585.1;Parent=MsaT044585.1
Chr8	exon	62319524	62319673	62319524	ID=MsaT044585.1.exon1;Parent=MsaT044585.1
Chr8	CDS	62319524	62319673	62319524	ID=cds.MsaT044585.1;Parent=MsaT044585.1

Gene Sequence

>MsaT044585.1

ATGGGTGCTCCTAAGCAGAAATGGACTGCAGAAGAAGAAGCGGCGTTGAAAGCTGGAGTAGTCAAACACGGGGCTGGAAAATGGCGCACAATACTCATGGATCCTGAGTTCAGTTCCATTTTGCGTACGCGTTCGAATGTAGATCTCAAGGATAAATGGAGGAATATAAATGTCACAGCAATATGGGGATCCCGGCAGAAAGCAAAACTTGCCCTTAAGAGCCTTCCAGCCCCCAAAACTGACAATAACCAATTGGCCTTGAGTAAAGTAGTTCAACGTGAAGACTTTCTCGATATTAAGCCCCTGACAATTTCTGGTGGAACATTGCAATCTCCTACGCCCCTAACTATTTGTAGTGGAACATTGCAATCTCCTAGTTCAAAAGAACAAGTATCAAGGTTGGGAGACAATAATGTATTAGAGGCTATTGTCAATATGAAGGAGCCAAAGGGTTCTGACAAGGCTGCCATTGCTTCTTACATAGAGGAAAAATATCAGTGTCCACCAAATCTTAGAAAGTTATTGTCAGCAAAATTGCAGCAGATGGTGACAAGTGGCAAAATAGTCCAGGAAAAGCACAAGTACAAAATTATGCCAAGTTCAGCGGTCTCTGAGAAAAGAGGAAGCTCTTCTCTGATGCTTGTGGAAGCGAGGTCCAAAGATTCTCCGGAGGTGGAGAAGATGTGTGATGTCAACATCCTTTCAAAATCCCAAATTGAGGCAGAGCTATCAAAAGTGAGGGGGATGTCAGCTCAAGAGGCGGCTGCTGCAGCTGCAAAAGCAGTTGCAGAGGCAGAAGTTGCCATTGCACAGGCCGAAGCAGCAGCTAGGGAGGCGGAGATTGCAGAAGCTGAAGCTGAGGCTGCTCAAGTCTTTGCAAAAGCAGCGATGAAGGCACTCAAATGCAAAATGCTTCATATTTGGTAG

Protein sequence

>MsaT044585.1

MGAPKQKWTAEEEAALKAGVVKHGAGKWRTILMDPEFSSILRTRSNVDLKDKWRNINVTAIWGSRQKAKLALKSLPAPKTDNNQLALSKVVQREDFLDIKPLTISGGTLQSPTPLTICSGTLQSPSSKEQVSRLGDNNVLEAIVNMKEPKGSDKAAIASYIEEKYQCPPNLRKLLSAKLQQMVTSGKIVQEKHKYKIMPSSAVSEKRGSSSLMLVEARSKDSPEVEKMCDVNILSKSQIEAELSKVRGMSAQEAAAAAAKAVAEAEVAIAQAEAAAREAEIAEAEAEAAQVFAKAAMKALKCKMLHIW*