AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044639

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044639 MtrunA17_Chr4g0054551 86.826 334 37 4 1 333 1 328 0.0 580
MsaG044639 MtrunA17_Chr4g0054561 86.527 334 38 4 1 333 1 328 0.0 577
MsaG044639 MtrunA17_Chr5g0400671 43.978 357 137 11 1 329 1 322 5.76e-78 241
MsaG044639 MtrunA17_Chr2g0280161 39.489 352 174 14 2 325 5 345 2.13e-57 189
MsaG044639 MtrunA17_Chr4g0049331 42.049 283 138 9 72 333 33 310 2.72e-56 185
MsaG044639 MtrunA17_Chr8g0336681 47.340 188 86 4 153 329 149 334 8.31e-51 172
MsaG044639 MtrunA17_Chr5g0400661 49.468 188 78 4 158 329 147 333 1.30e-50 171
MsaG044639 MtrunA17_Chr6g0468491 37.153 288 153 6 42 325 41 304 1.07e-48 166
MsaG044639 MtrunA17_Chr2g0307131 50.292 171 81 2 158 325 177 346 1.11e-48 166
MsaG044639 MtrunA17_Chr2g0307141 49.123 171 83 2 158 325 181 350 3.38e-48 166
MsaG044639 MtrunA17_Chr5g0400621 44.248 226 119 4 105 325 101 324 3.81e-48 164
MsaG044639 MtrunA17_Chr2g0333621 48.677 189 90 3 146 329 124 310 8.58e-48 163
MsaG044639 MtrunA17_Chr4g0054581 41.767 249 110 9 90 325 143 369 9.57e-47 162
MsaG044639 MtrunA17_Chr4g0033391 44.019 209 98 5 128 333 127 319 3.37e-46 159
MsaG044639 MtrunA17_Chr7g0225901 47.134 157 79 3 157 310 85 240 1.19e-43 150
MsaG044639 MtrunA17_Chr4g0033291 42.512 207 110 4 128 329 121 323 1.59e-43 152
MsaG044639 MtrunA17_Chr4g0002801 45.161 186 92 4 153 329 145 329 8.34e-43 150
MsaG044639 MtrunA17_Chr4g0002781 45.109 184 94 3 152 329 166 348 5.24e-41 146
MsaG044639 MtrunA17_Chr4g0054621 41.989 181 100 2 145 325 157 332 1.08e-40 145
MsaG044639 MtrunA17_Chr5g0400641 47.134 157 79 2 163 316 1 156 2.24e-40 139
MsaG044639 MtrunA17_Chr4g0054571 48.148 189 75 6 160 332 150 331 1.00e-39 142
MsaG044639 MtrunA17_Chr2g0333641 44.324 185 101 2 146 329 123 306 1.86e-39 141
MsaG044639 MtrunA17_Chr4g0033281 42.365 203 107 5 128 325 119 316 2.88e-38 139
MsaG044639 MtrunA17_Chr2g0333611 38.919 185 103 4 153 332 38 217 1.37e-35 128
MsaG044639 MtrunA17_Chr7g0249811 45.251 179 91 2 151 329 156 327 2.43e-35 131
MsaG044639 MtrunA17_Chr2g0333541 42.623 183 95 5 153 328 87 266 1.10e-31 120
MsaG044639 MtrunA17_Chr6g0468461 41.290 155 79 2 156 310 169 311 2.24e-30 118
MsaG044639 MtrunA17_Chr7g0233441 35.749 207 105 8 127 325 5 191 2.38e-22 92.8
MsaG044639 MtrunA17_Chr4g0002831 36.875 160 91 6 171 329 53 203 4.99e-21 89.4
MsaG044639 MtrunA17_Chr4g0002851 35.625 160 76 4 171 325 54 191 3.09e-20 87.0
MsaG044639 MtrunA17_Chr8g0346161 28.333 180 111 6 160 327 291 464 9.67e-16 77.8
MsaG044639 MtrunA17_Chr1g0185621 34.459 148 80 4 160 292 475 620 8.87e-15 75.1
MsaG044639 MtrunA17_Chr5g0411341 32.258 155 73 7 160 289 493 640 1.87e-13 71.2
MsaG044639 MtrunA17_Chr7g0260841 48.571 70 33 1 144 213 342 408 2.98e-13 70.5
MsaG044639 MtrunA17_Chr8g0346151 31.034 116 69 2 160 266 279 392 7.19e-13 68.9
MsaG044639 MtrunA17_Chr8g0366751 34.677 124 67 3 160 269 462 585 1.11e-12 68.9
MsaG044639 MtrunA17_Chr7g0275821 51.786 56 27 0 160 215 252 307 1.32e-12 68.2
MsaG044639 MtrunA17_Chr8g0365711 29.448 163 106 3 153 307 237 398 1.73e-12 67.8
MsaG044639 MtrunA17_Chr8g0386921 41.791 67 39 0 157 223 403 469 7.48e-12 66.2
MsaG044639 MtrunA17_Chr4g0028071 28.289 152 91 5 162 302 151 295 7.06e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044639 AT2G22760.1 51.667 180 79 5 153 325 112 290 5.42e-53 176
MsaG044639 AT4G37850.2 50.000 192 89 4 147 333 139 328 2.19e-52 176
MsaG044639 AT4G37850.1 50.000 192 89 4 147 333 139 328 2.19e-52 176
MsaG044639 AT2G22760.2 51.829 164 72 4 153 310 112 274 1.20e-47 162
MsaG044639 AT2G22770.1 44.690 226 95 6 129 329 98 318 3.46e-47 162
MsaG044639 AT2G22750.2 44.223 251 107 9 103 332 66 304 6.42e-47 161
MsaG044639 AT2G22750.3 44.223 251 107 9 103 332 94 332 8.74e-47 161
MsaG044639 AT2G22750.5 44.223 251 107 9 103 332 90 328 9.79e-47 161
MsaG044639 AT2G22750.1 44.223 251 106 10 103 332 66 303 1.81e-45 157
MsaG044639 AT2G22750.4 44.223 251 106 10 103 332 94 331 2.78e-45 157
MsaG044639 AT1G32640.1 36.691 139 78 6 160 289 452 589 1.33e-15 78.2
MsaG044639 AT2G46510.1 58.929 56 23 0 160 215 395 450 8.06e-15 75.9
MsaG044639 AT1G01260.1 26.556 241 142 7 27 267 316 521 5.48e-14 73.2
MsaG044639 AT1G01260.2 26.556 241 142 7 27 267 316 521 5.48e-14 73.2
MsaG044639 AT1G01260.3 26.556 241 142 7 27 267 316 521 5.48e-14 73.2
MsaG044639 AT5G46760.1 34.188 117 70 2 160 269 415 531 3.35e-12 67.8
MsaG044639 AT1G63650.3 35.955 89 56 1 155 242 400 488 4.10e-12 67.4
MsaG044639 AT1G63650.1 35.955 89 56 1 155 242 400 488 4.10e-12 67.4
MsaG044639 AT1G63650.2 35.955 89 56 1 155 242 400 488 4.10e-12 67.4
MsaG044639 AT4G16430.1 42.045 88 46 2 141 223 297 384 4.57e-12 67.0
MsaG044639 AT4G17880.1 33.333 120 63 3 160 269 416 528 5.60e-12 67.0
MsaG044639 AT4G09820.1 50.820 61 30 0 153 213 356 416 1.59e-11 65.5
MsaG044639 AT1G12860.1 29.518 166 101 6 161 314 268 429 5.88e-11 63.5
MsaG044639 AT1G12860.2 30.769 156 92 6 161 304 268 419 7.81e-11 63.2
MsaG044639 AT5G67110.1 36.842 95 51 2 122 212 60 149 8.46e-11 61.2
MsaG044639 AT2G16910.1 50.000 56 28 0 161 216 315 370 8.97e-11 63.2

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTGAAGCAAGGGTTTC+TGG 0.149381 8:-62962817 MsaT044639.1:CDS
GATGGACAAGGCAACTATTT+TGG 0.186609 8:-62963004 MsaT044639.1:CDS
TGCATTCCAGTTTGATGATA+TGG 0.223416 8:-62963642 MsaT044639.1:CDS
TTTGAATTAACAGGGATTAA+TGG 0.241351 8:-62963711 MsaT044639.1:intron
GCTCTTTCATCTCTTCTTCC+TGG 0.251328 8:-62963206 MsaT044639.1:CDS
TTCACCTTTGCATCCATGTT+TGG 0.279378 8:+62963407 None:intergenic
TCTTCTTCCTGGACTCAAAA+AGG 0.317343 8:-62963195 MsaT044639.1:intron
TGTTGTATACTTACAAATTC+AGG 0.318270 8:+62963942 None:intergenic
AGAATTGTTCTGTGTGCTTT+CGG 0.323320 8:+62963535 None:intergenic
AGAATATTGCTTGACAATAA+AGG 0.327738 8:-62962255 MsaT044639.1:CDS
TATGGAGAAAACTTGAATTT+CGG 0.349571 8:-62963362 MsaT044639.1:CDS
TGTAAATAGAAACCCTATTC+AGG 0.354615 8:-62963288 MsaT044639.1:CDS
AGCAGTTCTACTTTGCCATT+TGG 0.363549 8:-62962697 MsaT044639.1:CDS
ACATGATCTTGAGCCTGAAT+AGG 0.366395 8:+62963275 None:intergenic
CATGATCTTGAGCCTGAATA+GGG 0.371001 8:+62963276 None:intergenic
TCGCTTGTTGTTGATGTTGT+TGG 0.394714 8:+62963565 None:intergenic
TGATATAACAATTGTTGCTC+AGG 0.416686 8:-62962662 MsaT044639.1:intron
AGTATGCCATATCATCAAAC+TGG 0.434714 8:+62963636 None:intergenic
TTGACCAGGACTACTTTCAA+TGG 0.436916 8:+62963508 None:intergenic
ATGAAAGAGCAATGAATCTC+TGG 0.443321 8:+62963219 None:intergenic
TGACCAGGACTACTTTCAAT+GGG 0.452873 8:+62963509 None:intergenic
CAGCAGCAACCTTGTTCTCT+CGG 0.454650 8:+62963309 None:intergenic
TGAGTATTGCGGTCGCAGTT+CGG 0.460089 8:+62962752 None:intergenic
TTCCCCATTGAAAGTAGTCC+TGG 0.464737 8:-62963512 MsaT044639.1:CDS
TCAAGATAGTTATTTCCAAA+TGG 0.471769 8:+62962682 None:intergenic
AAGAAGCCTAAAACTGAGAT+CGG 0.487088 8:-62963386 MsaT044639.1:CDS
CCTAAAACTGAGATCGGGTA+TGG 0.488086 8:-62963380 MsaT044639.1:CDS
TGGAATGCATATGAATCCAT+TGG 0.489021 8:+62963656 None:intergenic
GGAACGAGTGAAGACTTTAG+AGG 0.491147 8:-62962950 MsaT044639.1:CDS
TACAAATTCAGGCATGTAGT+TGG 0.494277 8:+62963953 None:intergenic
TGGTTCAAAAGAAATGATCT+TGG 0.499628 8:+62963427 None:intergenic
AGGAATAAGTACTCTCTTGT+GGG 0.507070 8:+62963594 None:intergenic
TCAGGCTCAAGATCATGTTA+TGG 0.510343 8:-62963270 MsaT044639.1:CDS
ACTTCCGAAAATTGAAGCAA+GGG 0.522336 8:-62962824 MsaT044639.1:CDS
TCTCGTAAGGAGCATAAGTC+AGG 0.525482 8:-62962231 MsaT044639.1:CDS
CACTTCCGAAAATTGAAGCA+AGG 0.531807 8:-62962825 MsaT044639.1:CDS
TGTTCTGTGTGCTTTCGGAA+TGG 0.533620 8:+62963540 None:intergenic
AAATTCAGGCATGTAGTTGG+TGG 0.541463 8:+62963956 None:intergenic
GTTGATGTTGTTGGTATGAG+AGG 0.543066 8:+62963574 None:intergenic
GAGGAATAAGTACTCTCTTG+TGG 0.546666 8:+62963593 None:intergenic
AGAAGCCTAAAACTGAGATC+GGG 0.557163 8:-62963385 MsaT044639.1:CDS
TGAACCAAACATGGATGCAA+AGG 0.563886 8:-62963411 MsaT044639.1:CDS
AAAACATATGAAGCAACTAC+AGG 0.565504 8:-62962971 MsaT044639.1:CDS
ATCCATTGTGACAAATATTG+TGG 0.568305 8:-62962775 MsaT044639.1:CDS
TTCGGAAGTGTGATATCTCA+AGG 0.572553 8:-62963344 MsaT044639.1:CDS
TTCTAATTCGATGAGTATTG+CGG 0.578276 8:+62962741 None:intergenic
GGCCACAATATTTGTCACAA+TGG 0.583000 8:+62962773 None:intergenic
TAGTGATCATGAATATCCAA+TGG 0.584018 8:-62963672 MsaT044639.1:CDS
TGACAATAAAGGATCTCGTA+AGG 0.599743 8:-62962244 MsaT044639.1:CDS
TTATTATATCCGAGAGAACA+AGG 0.602794 8:-62963318 MsaT044639.1:CDS
GAATTAACAGGGATTAATGG+AGG 0.608513 8:-62963708 MsaT044639.1:intron
GGAATAAGTACTCTCTTGTG+GGG 0.633544 8:+62963595 None:intergenic
ACTTTGCATGCAGATGGACA+AGG 0.646531 8:-62963016 MsaT044639.1:intron
TATGGCAGAAAGAAGACGAA+GGG 0.657033 8:-62963252 MsaT044639.1:CDS
GCTGAAGCAACAGATTGACC+AGG 0.668418 8:+62963494 None:intergenic
TTATGGCAGAAAGAAGACGA+AGG 0.702887 8:-62963253 MsaT044639.1:CDS
GACCAGGACTACTTTCAATG+GGG 0.715740 8:+62963510 None:intergenic
TAGAACTGCTCTGAACAGTG+AGG 0.757817 8:+62962710 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 62962211 62963994 62962211 ID=MsaG044639
Chr8 mRNA 62962211 62963994 62962211 ID=MsaT044639.1;Parent=MsaG044639
Chr8 exon 62962211 62962285 62962211 ID=MsaT044639.1.exon4;Parent=MsaT044639.1
Chr8 CDS 62962211 62962285 62962211 ID=cds.MsaT044639.1;Parent=MsaT044639.1
Chr8 exon 62962663 62963025 62962663 ID=MsaT044639.1.exon3;Parent=MsaT044639.1
Chr8 CDS 62962663 62963025 62962663 ID=cds.MsaT044639.1;Parent=MsaT044639.1
Chr8 exon 62963196 62963720 62963196 ID=MsaT044639.1.exon2;Parent=MsaT044639.1
Chr8 CDS 62963196 62963720 62963196 ID=cds.MsaT044639.1;Parent=MsaT044639.1
Chr8 exon 62963956 62963994 62963956 ID=MsaT044639.1.exon1;Parent=MsaT044639.1
Chr8 CDS 62963956 62963994 62963956 ID=cds.MsaT044639.1;Parent=MsaT044639.1
Gene Sequence

>MsaT044639.1

ATGATTCAAATTTCGTCCACCAACTACATGCCTGAATTTGGATTAATGGAGGACAATACTTTGTTTAGTGATCATGAATATCCAATGGATTCATATGCATTCCAGTTTGATGATATGGCATACTTCAAGTCTTTCTCAGCATCCCCACAAGAGAGTACTTATTCCTCTCATACCAACAACATCAACAACAAGCGAATCCATTCCGAAAGCACACAGAACAATTCTTTCCCCATTGAAAGTAGTCCTGGTCAATCTGTTGCTTCAGCTACACCAACAAAACTAAGACTCAAGTCTTCTTCATCTTCATCTTCCAAGATCATTTCTTTTGAACCAAACATGGATGCAAAGGTGAAGAAGCCTAAAACTGAGATCGGGTATGGAGAAAACTTGAATTTCGGAAGTGTGATATCTCAAGGTGATTATTATATCCGAGAGAACAAGGTTGCTGCTGTAAATAGAAACCCTATTCAGGCTCAAGATCATGTTATGGCAGAAAGAAGACGAAGGGAAAAGCTTAGCCAGAGATTCATTGCTCTTTCATCTCTTCTTCCTGGACTCAAAAAGATGGACAAGGCAACTATTTTGGAAGATGCTATAAAACATATGAAGCAACTACAGGAACGAGTGAAGACTTTAGAGGAACAAGTAACCAATAAAAAAGTCGAATCAGCTGTTTTTGTGAAGAGATCTATTCTCTTTGACGAAGATGATAGCTCATCTTGCGACAAAAATTCAGACCAATCACTTCCGAAAATTGAAGCAAGGGTTTCTGGAAAAGACATGCTCATCAGAATCCATTGTGACAAATATTGTGGCCGAACTGCGACCGCAATACTCATCGAATTAGAAAAGCATCACCTCACTGTTCAGAGCAGTTCTACTTTGCCATTTGGAAATAACTATCTTGATATAACAATTGTTGCTCAGATGAATAAAGAATATTGCTTGACAATAAAGGATCTCGTAAGGAGCATAAGTCAGGTTTTGAGGCAGCTCATTTGA
Protein sequence

>MsaT044639.1

MIQISSTNYMPEFGLMEDNTLFSDHEYPMDSYAFQFDDMAYFKSFSASPQESTYSSHTNNINNKRIHSESTQNNSFPIESSPGQSVASATPTKLRLKSSSSSSSKIISFEPNMDAKVKKPKTEIGYGENLNFGSVISQGDYYIRENKVAAVNRNPIQAQDHVMAERRRREKLSQRFIALSSLLPGLKKMDKATILEDAIKHMKQLQERVKTLEEQVTNKKVESAVFVKRSILFDEDDSSSCDKNSDQSLPKIEARVSGKDMLIRIHCDKYCGRTATAILIELEKHHLTVQSSSTLPFGNNYLDITIVAQMNKEYCLTIKDLVRSISQVLRQLI*