AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044640 MtrunA17_Chr4g0054561 88.854 323 29 3 301 623 13 328 0.0 583
MsaG044640 MtrunA17_Chr4g0054561 44.751 181 81 4 121 297 155 320 1.98e-38 144
MsaG044640 MtrunA17_Chr4g0054551 86.687 323 36 3 301 623 13 328 0.0 570
MsaG044640 MtrunA17_Chr4g0054551 41.989 181 86 4 121 297 155 320 4.62e-34 132
MsaG044640 MtrunA17_Chr4g0054571 92.466 292 19 2 11 301 39 328 4.37e-178 508
MsaG044640 MtrunA17_Chr4g0054571 47.059 187 78 6 450 621 150 330 2.26e-35 136
MsaG044640 MtrunA17_Chr5g0400671 45.190 343 131 13 301 619 13 322 2.21e-72 236
MsaG044640 MtrunA17_Chr5g0400671 30.721 319 176 5 6 301 26 322 1.98e-31 125
MsaG044640 MtrunA17_Chr5g0400661 44.985 329 132 10 11 301 16 333 5.40e-67 222
MsaG044640 MtrunA17_Chr5g0400661 36.624 314 154 10 331 619 40 333 2.47e-45 164
MsaG044640 MtrunA17_Chr5g0400621 44.689 273 124 7 32 300 78 327 4.09e-51 179
MsaG044640 MtrunA17_Chr5g0400621 50.000 170 81 2 449 615 156 324 4.00e-40 149
MsaG044640 MtrunA17_Chr2g0280161 38.012 342 173 13 301 615 16 345 1.78e-49 176
MsaG044640 MtrunA17_Chr2g0280161 52.514 179 75 4 121 298 177 346 2.56e-48 172
MsaG044640 MtrunA17_Chr4g0049331 45.417 240 121 6 388 623 77 310 3.75e-49 173
MsaG044640 MtrunA17_Chr4g0049331 42.910 268 123 8 38 298 59 303 7.03e-48 170
MsaG044640 MtrunA17_Chr8g0336681 46.809 188 87 4 443 619 149 334 1.79e-47 170
MsaG044640 MtrunA17_Chr8g0336681 39.370 254 131 7 59 301 93 334 4.86e-36 138
MsaG044640 MtrunA17_Chr2g0333621 47.396 192 94 3 433 619 121 310 1.76e-44 161
MsaG044640 MtrunA17_Chr2g0333621 40.467 257 132 8 50 298 64 307 2.39e-40 149
MsaG044640 MtrunA17_Chr6g0468491 47.093 172 87 3 447 615 134 304 3.62e-44 160
MsaG044640 MtrunA17_Chr6g0468491 45.213 188 92 3 113 299 129 306 2.41e-41 152
MsaG044640 MtrunA17_Chr4g0054581 42.636 258 118 8 66 300 122 372 5.34e-44 161
MsaG044640 MtrunA17_Chr4g0054581 44.550 211 93 6 414 615 174 369 1.60e-42 157
MsaG044640 MtrunA17_Chr4g0002801 40.833 240 120 6 65 298 103 326 7.83e-42 154
MsaG044640 MtrunA17_Chr4g0002801 40.816 245 121 7 386 619 98 329 1.44e-40 150
MsaG044640 MtrunA17_Chr4g0033391 43.062 209 100 5 418 623 127 319 3.25e-41 152
MsaG044640 MtrunA17_Chr4g0033391 44.149 188 85 3 118 301 144 315 1.35e-38 145
MsaG044640 MtrunA17_Chr4g0002781 40.873 252 115 9 386 619 113 348 9.19e-41 152
MsaG044640 MtrunA17_Chr4g0002781 37.021 235 140 3 65 298 118 345 8.12e-40 149
MsaG044640 MtrunA17_Chr2g0307131 47.368 171 86 2 448 615 177 346 9.21e-41 152
MsaG044640 MtrunA17_Chr2g0307131 43.415 205 105 3 95 298 153 347 1.83e-40 151
MsaG044640 MtrunA17_Chr2g0307141 46.199 171 88 2 448 615 181 350 1.78e-40 151
MsaG044640 MtrunA17_Chr2g0307141 45.810 179 86 3 121 298 183 351 1.85e-37 142
MsaG044640 MtrunA17_Chr4g0033291 42.995 207 109 4 418 619 121 323 3.76e-40 149
MsaG044640 MtrunA17_Chr4g0033291 44.737 190 93 2 112 301 146 323 5.09e-38 143
MsaG044640 MtrunA17_Chr7g0225901 47.134 157 79 3 447 600 85 240 1.13e-39 145
MsaG044640 MtrunA17_Chr7g0225901 45.930 172 82 3 113 283 80 241 4.51e-39 144
MsaG044640 MtrunA17_Chr5g0400641 47.273 165 78 3 124 288 1 156 4.73e-39 141
MsaG044640 MtrunA17_Chr5g0400641 44.099 161 78 4 453 606 1 156 7.37e-36 132
MsaG044640 MtrunA17_Chr2g0333641 43.085 188 105 2 433 619 120 306 4.02e-37 140
MsaG044640 MtrunA17_Chr2g0333641 39.662 237 125 8 65 297 80 302 1.21e-33 130
MsaG044640 MtrunA17_Chr4g0054621 41.989 181 100 2 435 615 157 332 1.00e-36 140
MsaG044640 MtrunA17_Chr4g0054621 35.662 272 149 6 30 300 89 335 3.90e-35 135
MsaG044640 MtrunA17_Chr4g0033281 41.089 202 111 4 418 615 119 316 3.17e-34 133
MsaG044640 MtrunA17_Chr4g0033281 42.932 191 94 3 112 301 144 320 1.20e-32 128
MsaG044640 MtrunA17_Chr7g0249811 44.134 179 93 2 441 619 156 327 5.45e-34 132
MsaG044640 MtrunA17_Chr7g0249811 33.475 236 129 3 63 298 117 324 5.46e-28 115
MsaG044640 MtrunA17_Chr2g0333611 39.674 184 97 6 443 619 38 214 2.27e-33 127
MsaG044640 MtrunA17_Chr2g0333611 39.459 185 91 4 122 301 46 214 1.11e-30 119
MsaG044640 MtrunA17_Chr6g0468461 35.401 274 137 7 16 282 71 311 1.27e-30 123
MsaG044640 MtrunA17_Chr6g0468461 40.645 155 80 2 446 600 169 311 9.44e-27 112
MsaG044640 MtrunA17_Chr2g0333541 41.622 185 94 6 443 618 87 266 3.03e-29 117
MsaG044640 MtrunA17_Chr2g0333541 38.500 200 110 4 101 299 78 265 1.30e-26 109
MsaG044640 MtrunA17_Chr7g0233441 34.177 158 85 4 144 301 57 195 9.16e-20 87.8
MsaG044640 MtrunA17_Chr7g0233441 38.095 147 82 4 469 615 54 191 1.64e-19 87.0
MsaG044640 MtrunA17_Chr8g0365711 31.844 179 118 2 101 279 224 398 1.69e-19 91.3
MsaG044640 MtrunA17_Chr4g0002831 33.149 181 99 4 120 299 42 201 1.86e-19 87.0
MsaG044640 MtrunA17_Chr4g0002831 36.250 160 92 5 461 619 53 203 2.27e-19 87.0
MsaG044640 MtrunA17_Chr4g0002851 31.176 170 89 3 132 301 54 195 2.26e-19 86.7
MsaG044640 MtrunA17_Chr4g0002851 34.375 160 78 4 461 615 54 191 1.64e-18 84.3
MsaG044640 MtrunA17_Chr8g0346161 29.557 203 119 8 431 617 270 464 2.04e-15 79.3
MsaG044640 MtrunA17_Chr8g0346161 30.481 187 111 6 106 287 276 448 4.85e-15 78.2
MsaG044640 MtrunA17_Chr8g0366751 35.762 151 87 3 121 265 462 608 4.27e-15 79.0
MsaG044640 MtrunA17_Chr8g0366751 31.928 166 95 5 433 584 448 609 5.17e-12 68.9
MsaG044640 MtrunA17_Chr7g0275821 54.237 59 27 0 121 179 252 310 2.39e-14 75.5
MsaG044640 MtrunA17_Chr7g0275821 50.000 56 28 0 450 505 252 307 9.88e-12 67.4
MsaG044640 MtrunA17_Chr1g0185621 30.000 150 96 4 121 266 475 619 2.07e-13 73.6
MsaG044640 MtrunA17_Chr1g0185621 50.000 64 30 1 450 513 475 536 3.06e-13 72.8
MsaG044640 MtrunA17_Chr8g0346151 28.144 167 102 5 431 584 258 419 2.75e-13 72.4
MsaG044640 MtrunA17_Chr8g0346151 31.447 159 99 3 80 238 243 391 4.84e-13 71.6
MsaG044640 MtrunA17_Chr7g0260841 55.556 54 24 0 121 174 355 408 3.26e-13 72.4
MsaG044640 MtrunA17_Chr7g0260841 46.250 80 38 2 433 510 341 417 4.59e-13 72.0
MsaG044640 MtrunA17_Chr5g0411341 43.023 86 49 0 89 174 461 546 6.68e-13 72.0
MsaG044640 MtrunA17_Chr5g0411341 30.909 165 93 5 433 579 479 640 7.54e-12 68.6
MsaG044640 MtrunA17_Chr2g0320291 28.415 183 116 4 123 304 135 303 2.30e-12 68.6
MsaG044640 MtrunA17_Chr3g0131691 26.768 198 117 6 89 276 275 454 7.88e-12 68.2
MsaG044640 MtrunA17_Chr2g0298751 28.571 182 113 6 122 291 53 229 2.48e-11 64.3
MsaG044640 MtrunA17_Chr8g0386921 41.791 67 39 0 447 513 403 469 4.41e-11 65.9
MsaG044640 MtrunA17_Chr7g0252041 26.875 160 110 3 122 276 287 444 6.52e-11 65.1
MsaG044640 MtrunA17_Chr4g0028071 26.250 160 102 4 123 278 151 298 6.69e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044640 AT4G37850.2 39.873 316 158 10 4 301 23 324 5.05e-51 179
MsaG044640 AT4G37850.2 46.798 203 99 5 428 623 128 328 1.96e-47 170
MsaG044640 AT4G37850.1 39.873 316 158 10 4 301 23 324 5.05e-51 179
MsaG044640 AT4G37850.1 46.798 203 99 5 428 623 128 328 1.96e-47 170
MsaG044640 AT2G22760.1 50.000 180 82 5 443 615 112 290 1.67e-48 171
MsaG044640 AT2G22760.1 40.796 201 104 5 109 301 101 294 5.44e-37 140
MsaG044640 AT2G22760.2 50.000 164 75 4 443 600 112 274 2.04e-43 157
MsaG044640 AT2G22760.2 40.884 181 93 4 109 282 101 274 1.19e-32 127
MsaG044640 AT2G22770.1 43.439 221 103 5 420 619 99 318 1.15e-39 148
MsaG044640 AT2G22770.1 38.021 192 113 2 117 302 128 319 5.01e-34 132
MsaG044640 AT2G22750.2 46.277 188 91 3 437 616 113 298 6.97e-39 145
MsaG044640 AT2G22750.2 40.925 281 137 8 36 301 35 301 9.90e-38 142
MsaG044640 AT2G22750.3 46.277 188 91 3 437 616 141 326 8.10e-39 146
MsaG044640 AT2G22750.3 38.486 317 160 9 5 301 28 329 1.64e-38 145
MsaG044640 AT2G22750.5 46.277 188 91 3 437 616 137 322 9.47e-39 146
MsaG044640 AT2G22750.5 38.486 317 160 9 5 301 24 325 2.07e-38 145
MsaG044640 AT2G22750.1 46.277 188 90 4 437 616 113 297 1.50e-37 142
MsaG044640 AT2G22750.1 40.925 281 136 9 36 301 35 300 1.85e-36 139
MsaG044640 AT2G22750.4 46.277 188 90 4 437 616 141 325 1.79e-37 142
MsaG044640 AT2G22750.4 38.486 317 159 10 5 301 28 328 4.11e-37 141
MsaG044640 AT4G29930.1 29.000 200 115 5 110 285 33 229 4.06e-16 79.0
MsaG044640 AT4G29930.3 28.934 197 113 5 110 282 33 226 1.48e-15 77.8
MsaG044640 AT4G29930.7 28.934 197 113 5 110 282 33 226 1.48e-15 77.8
MsaG044640 AT2G46510.1 58.929 56 23 0 121 176 395 450 2.02e-15 80.1
MsaG044640 AT2G46510.1 47.561 82 40 1 424 505 372 450 1.56e-14 77.4
MsaG044640 AT1G32640.1 36.709 158 83 7 433 579 438 589 3.70e-15 79.3
MsaG044640 AT1G32640.1 31.875 160 101 2 106 265 437 588 1.53e-13 74.3
MsaG044640 AT5G46760.1 34.437 151 82 4 121 265 415 554 3.72e-15 79.3
MsaG044640 AT5G46760.1 31.481 162 94 5 433 584 401 555 9.01e-12 68.6
MsaG044640 AT4G29930.2 27.895 190 101 5 110 285 33 200 3.46e-14 72.8
MsaG044640 AT4G29930.6 28.571 189 104 5 110 285 33 203 4.28e-14 72.4
MsaG044640 AT5G57150.1 27.807 187 129 3 122 307 55 236 7.78e-14 72.0
MsaG044640 AT4G29930.5 29.101 189 94 6 110 282 33 197 9.54e-14 71.6
MsaG044640 AT2G16910.1 27.528 178 116 3 122 286 315 492 2.38e-13 73.6
MsaG044640 AT1G01260.1 31.293 147 64 3 121 264 433 545 2.93e-13 73.2
MsaG044640 AT1G01260.1 49.153 59 30 0 447 505 430 488 1.15e-11 68.2
MsaG044640 AT1G01260.2 31.293 147 64 3 121 264 433 545 2.93e-13 73.2
MsaG044640 AT1G01260.2 49.153 59 30 0 447 505 430 488 1.15e-11 68.2
MsaG044640 AT1G01260.3 31.293 147 64 3 121 264 433 545 2.93e-13 73.2
MsaG044640 AT1G01260.3 49.153 59 30 0 447 505 430 488 1.15e-11 68.2
MsaG044640 AT5G58010.1 40.741 108 57 2 119 222 107 211 4.26e-13 70.9
MsaG044640 AT5G57150.3 30.667 150 100 3 122 268 56 204 5.74e-13 68.9
MsaG044640 AT5G57150.2 30.667 150 100 3 122 268 56 204 6.11e-13 69.3
MsaG044640 AT5G57150.5 30.667 150 100 3 122 268 55 203 6.16e-13 68.9
MsaG044640 AT4G17880.1 32.468 154 77 3 121 265 416 551 7.16e-13 72.0
MsaG044640 AT4G17880.1 31.447 159 94 5 433 584 402 552 1.49e-11 67.8
MsaG044640 AT5G57150.4 30.667 150 100 3 122 268 56 204 1.44e-12 68.9
MsaG044640 AT5G41315.1 28.161 174 110 3 119 278 439 611 1.55e-12 70.9
MsaG044640 AT5G41315.3 28.161 174 110 3 119 278 444 616 1.56e-12 70.9
MsaG044640 AT5G41315.2 28.161 174 110 3 119 278 447 619 1.62e-12 70.9
MsaG044640 AT4G16430.1 36.250 80 51 0 99 178 298 377 9.60e-12 68.2
MsaG044640 AT4G16430.1 34.532 139 72 5 373 503 246 373 3.11e-11 66.6
MsaG044640 AT1G12860.2 29.375 160 100 3 122 276 268 419 1.38e-11 67.4
MsaG044640 AT1G12860.1 28.402 169 108 3 122 285 268 428 1.51e-11 67.4
MsaG044640 AT1G63650.3 26.590 173 117 4 115 278 399 570 3.14e-11 66.6
MsaG044640 AT1G63650.3 44.068 59 33 0 445 503 400 458 6.01e-11 65.9
MsaG044640 AT1G63650.1 26.590 173 117 4 115 278 399 570 3.14e-11 66.6
MsaG044640 AT1G63650.1 44.068 59 33 0 445 503 400 458 6.01e-11 65.9
MsaG044640 AT1G63650.2 26.590 173 117 4 115 278 399 570 3.14e-11 66.6
MsaG044640 AT1G63650.2 44.068 59 33 0 445 503 400 458 6.01e-11 65.9
MsaG044640 AT4G09820.1 27.933 179 105 5 443 619 356 512 3.15e-11 66.6
MsaG044640 AT4G09820.1 27.586 145 73 3 122 266 364 476 7.02e-11 65.5
MsaG044640 AT4G29930.4 32.203 118 68 2 110 217 33 148 6.69e-11 62.4
MsaG044640 AT2G28160.1 28.125 160 80 4 123 268 133 271 7.31e-11 64.3
MsaG044640 AT2G28160.2 28.125 160 80 4 123 268 133 271 7.56e-11 64.3

Find 108 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATCAAATGAAATGATTT+TGG 0.105789 8:+62990962 None:intergenic
TGCATTTCAGTTTGATGATA+TGG 0.178721 8:-62991165 MsaT044640.1:CDS
GATGGACAAGGCAACTATTT+TGG 0.186609 8:-62990514 MsaT044640.1:CDS
TACGGAGAAAACTTGAATTT+TGG 0.226888 8:-62990879 MsaT044640.1:CDS
TTTGAATTAACAGGGATTAA+TGG 0.241351 8:-62991234 MsaT044640.1:intron
GCTCTTTCCTCCATTCTTCC+TGG 0.259749 8:-62990723 MsaT044640.1:CDS
AAAATCGAAGCAAGAGTTTC+TGG 0.260282 8:-62990327 MsaT044640.1:CDS
CATTCTTCCTGGACTGAAAA+AGG 0.273836 8:-62990712 MsaT044640.1:intron
GGCCGCAATACTCAACTTAT+TGG 0.296853 8:-62990253 MsaT044640.1:CDS
ATATAATTGCTGAGAGAATA+AGG 0.332685 8:-62996939 MsaT044640.1:CDS
CCTTATTCCAAACCTCAAAA+AGG 0.354140 8:-62996878 MsaT044640.1:intron
GGAATATTGCTTAACAATAA+AGG 0.354244 8:-62990038 MsaT044640.1:CDS
TGTAAATAGAAACCCTATTC+AGG 0.354615 8:-62990805 MsaT044640.1:CDS
TCCTGATTCTTTACACGTCT+TGG 0.356377 8:+62997018 None:intergenic
ACATGATCTTGAGCCTGAAT+AGG 0.366395 8:+62990792 None:intergenic
TTCATTTGGTTCCTCTGCTC+TGG 0.370847 8:-62996021 MsaT044640.1:CDS
CATGATCTTGAGCCTGAATA+GGG 0.371001 8:+62990793 None:intergenic
CGGGGAAGAATGTGCTGATT+AGG 0.377824 8:-62996133 MsaT044640.1:CDS
GAACATGAGCATCCAGAAAC+TGG 0.387862 8:-62995111 MsaT044640.1:CDS
TCGCTTGTTGTTGATGTTGT+TGG 0.394714 8:+62991088 None:intergenic
GAAGTTAAAGCTAGTTTGTC+GGG 0.404928 8:-62996152 MsaT044640.1:CDS
AATTTCTTCACAACCGTAAC+CGG 0.405974 8:+62996272 None:intergenic
TTTCCGCGTTTATGCTTGAT+TGG 0.407164 8:+62997247 None:intergenic
AATAGAGAAACTTCATCTCT+TGG 0.413889 8:-62996063 MsaT044640.1:CDS
AGCATAAACGCGGAAATTGT+TGG 0.417963 8:-62997240 MsaT044640.1:CDS
TGATCTGGACTACTTTCAAT+GGG 0.422677 8:+62991032 None:intergenic
TGATATAACCATTGTTGCTC+AGG 0.429067 8:-62990172 MsaT044640.1:intron
AAGGTTGGTACAACCTTCAT+TGG 0.432513 8:+62997078 None:intergenic
TTGATCTGGACTACTTTCAA+TGG 0.434031 8:+62991031 None:intergenic
CTCTCAGCAATTATATGATC+TGG 0.438168 8:+62996946 None:intergenic
ATTCTTTGAGTTCACAACTT+TGG 0.438205 8:+62997044 None:intergenic
GACAAACTAGCTTTAACTTC+AGG 0.442233 8:+62996155 None:intergenic
GTCTGCGGATGTCAATGTTT+CGG 0.442533 8:-62996240 MsaT044640.1:CDS
ATGGACAAGGCCTCGGTGTT+GGG 0.445192 8:-62996374 MsaT044640.1:CDS
CAAAAGCAAAAGTGTAGAAC+CGG 0.446629 8:-62996291 MsaT044640.1:CDS
TGTTCTGTGGGCTTTCAGAA+TGG 0.448273 8:+62991063 None:intergenic
ACCAAGACGTGTAAAGAATC+AGG 0.456019 8:-62997019 MsaT044640.1:CDS
GTTTCCATTACATGAATTTG+AGG 0.464190 8:+62996211 None:intergenic
GGTACAACCTTCATTGGTGG+TGG 0.464980 8:+62997084 None:intergenic
AATTCATATGAATAGCATAA+AGG 0.468791 8:-62999137 MsaT044640.1:intron
AAAGTATGTGAAGGAGCTTA+AGG 0.470002 8:-62996342 MsaT044640.1:CDS
TTGATGAAACTGTAATCATT+TGG 0.470599 8:+62997270 None:intergenic
CAAAAGTGTAGAACCGGTTA+CGG 0.471591 8:-62996285 MsaT044640.1:CDS
ACTTCCTCAAATTCATGTAA+TGG 0.472899 8:-62996215 MsaT044640.1:CDS
GGAACAAGTAGCCGACAAAA+AGG 0.474291 8:-62990439 MsaT044640.1:CDS
TCAAGATAGTTATTTCCAAA+TGG 0.476177 8:+62990192 None:intergenic
AGGAAAGAGCAATGAATCTC+TGG 0.478665 8:+62990736 None:intergenic
GCCTTGAGTACTTTAGTTGG+CGG 0.485847 8:+62990990 None:intergenic
TGTGTGATGAGTTGTAGATT+TGG 0.488926 8:+62996969 None:intergenic
AAACTGTACCTGGGCAATGA+TGG 0.489078 8:+62995992 None:intergenic
AAGAAGCTTAAAACCGAGAT+CGG 0.489570 8:-62990903 MsaT044640.1:CDS
GTTTAGAGAGTAACTTAGAA+AGG 0.489805 8:-62997215 MsaT044640.1:CDS
GATGGACAAGGCCTCGGTGT+TGG 0.491255 8:-62996375 MsaT044640.1:CDS
GAAGCCTTGAGTACTTTAGT+TGG 0.494828 8:+62990987 None:intergenic
AAAGTTGTGAACTCAAAGAA+TGG 0.494937 8:-62997042 MsaT044640.1:CDS
TAATGATTATGAGTACCCTA+TGG 0.498020 8:-62991195 MsaT044640.1:CDS
TTTGTAGATGGACAAGGCCT+CGG 0.501079 8:-62996381 MsaT044640.1:intron
CGAGAATCCACCACCAATGA+AGG 0.502517 8:-62997091 MsaT044640.1:CDS
AGGAATAAGTACTCTCTTGT+GGG 0.507070 8:+62991117 None:intergenic
GCTGAGACAACAGATTGATC+TGG 0.515599 8:+62991017 None:intergenic
CTTAAAACCGAGATCGGGTA+CGG 0.516391 8:-62990897 MsaT044640.1:CDS
GGAACGAGTGAAGACATTAG+AGG 0.517587 8:-62990460 MsaT044640.1:CDS
TCAGGCTCAAGATCATGTTA+TGG 0.518678 8:-62990787 MsaT044640.1:CDS
TCTATTCTCTTCGACGATGA+TGG 0.521622 8:-62990390 MsaT044640.1:CDS
TGAAATGCAGATGAATCCAT+AGG 0.526233 8:+62991179 None:intergenic
TAAGGAACAAGTAAAACTGT+TGG 0.530262 8:-62996324 MsaT044640.1:CDS
CTGAAAGAGCTATGAACTGT+TGG 0.532934 8:+62996902 None:intergenic
TGAAGTTAAAGCTAGTTTGT+CGG 0.533689 8:-62996153 MsaT044640.1:CDS
TCTCGTAAGAAGCATAAGTC+AGG 0.535454 8:-62990014 MsaT044640.1:CDS
TAAACATATGAAGCAACTAA+AGG 0.541156 8:-62990481 MsaT044640.1:CDS
GTTGATGTTGTTGGTATGAG+AGG 0.543066 8:+62991097 None:intergenic
GAGGAATAAGTACTCTCTTG+TGG 0.546666 8:+62991116 None:intergenic
AGAAGCTTAAAACCGAGATC+GGG 0.550283 8:-62990902 MsaT044640.1:CDS
GGAACAAGTAAAACTGTTGG+AGG 0.550452 8:-62996321 MsaT044640.1:CDS
TTTGGAAGTGTGATATCTCA+AGG 0.551675 8:-62990861 MsaT044640.1:CDS
GGCCATAACCATCATTGCCC+AGG 0.553840 8:-62996000 MsaT044640.1:intron
TACCTGGGCAATGATGGTTA+TGG 0.564191 8:+62995998 None:intergenic
GTTGGTACAACCTTCATTGG+TGG 0.564633 8:+62997081 None:intergenic
TTCCAATAAGTTGAGTATTG+CGG 0.565334 8:+62990251 None:intergenic
TGGGGAAAGAACTGTTCTGT+GGG 0.565404 8:+62991051 None:intergenic
TGAGTATTGCGGCCGCAGAT+CGG 0.574788 8:+62990262 None:intergenic
GATCCTCTGTGAGAAAGATA+AGG 0.589750 8:-62996111 MsaT044640.1:CDS
ACCGCCAACTAAAGTACTCA+AGG 0.590049 8:-62990991 MsaT044640.1:CDS
ATGGGGAAAGAACTGTTCTG+TGG 0.590789 8:+62991050 None:intergenic
GAAATGCAGATGAATCCATA+GGG 0.600361 8:+62991180 None:intergenic
GAATTAACAGGGATTAATGG+AGG 0.608513 8:-62991231 MsaT044640.1:intron
GACATGCTCATCAGAATCCA+CGG 0.608624 8:-62990300 MsaT044640.1:CDS
GATGGTTATGGCCAGAGCAG+AGG 0.614054 8:+62996010 None:intergenic
TTATTATAAACGAGAGAACA+AGG 0.617033 8:-62990835 MsaT044640.1:CDS
GGCCGCAATGTTTATCACCG+TGG 0.620508 8:+62990283 None:intergenic
TGAGAAAGATAAGGCAGTGA+TGG 0.630057 8:-62996102 MsaT044640.1:CDS
TAAACATTGCGGCCGATCTG+CGG 0.633204 8:-62990274 MsaT044640.1:CDS
GGAATAAGTACTCTCTTGTG+GGG 0.633544 8:+62991118 None:intergenic
CAGGAGAGCAACAAGAAGAA+CGG 0.634139 8:-62997000 MsaT044640.1:CDS
TTTCAGTCCAGGAAGAATGG+AGG 0.639981 8:+62990716 None:intergenic
AAGTTAAAGCTAGTTTGTCG+GGG 0.643559 8:-62996151 MsaT044640.1:CDS
ACTTTGCATGCAGATGGACA+AGG 0.646531 8:-62990526 MsaT044640.1:intron
GAAATTGTCTGAACTGTCTG+CGG 0.654800 8:-62996255 MsaT044640.1:CDS
TATGGCCGAAAGAAGACGAA+GGG 0.657033 8:-62990769 MsaT044640.1:CDS
TGAAGGTTGTACCAACCTTG+AGG 0.663007 8:-62997074 MsaT044640.1:CDS
CATATGTTTGTAGATGGACA+AGG 0.664887 8:-62996387 MsaT044640.1:intron
CAACCAATCAAGCATAAACG+CGG 0.676384 8:-62997250 MsaT044640.1:CDS
CTGCCTTATCTTTCTCACAG+AGG 0.691029 8:+62996108 None:intergenic
TGATGCTATAAAGTATGTGA+AGG 0.702262 8:-62996351 MsaT044640.1:CDS
TTATGGCCGAAAGAAGACGA+AGG 0.705183 8:-62990770 MsaT044640.1:CDS
GATCTGGACTACTTTCAATG+GGG 0.710553 8:+62991033 None:intergenic
ATCCACGGTGATAAACATTG+CGG 0.719186 8:-62990285 MsaT044640.1:CDS
TATAGCATCACCCAACACCG+AGG 0.755825 8:+62996364 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 62989994 62999167 62989994 ID=MsaG044640
Chr8 mRNA 62989994 62999167 62989994 ID=MsaT044640.1;Parent=MsaG044640
Chr8 exon 62989994 62990068 62989994 ID=MsaT044640.1.exon7;Parent=MsaT044640.1
Chr8 CDS 62989994 62990068 62989994 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Chr8 exon 62990173 62990535 62990173 ID=MsaT044640.1.exon6;Parent=MsaT044640.1
Chr8 CDS 62990173 62990535 62990173 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Chr8 exon 62990713 62991243 62990713 ID=MsaT044640.1.exon5;Parent=MsaT044640.1
Chr8 CDS 62990713 62991243 62990713 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Chr8 exon 62995088 62995147 62995088 ID=MsaT044640.1.exon4;Parent=MsaT044640.1
Chr8 CDS 62995088 62995147 62995088 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Chr8 exon 62996001 62996396 62996001 ID=MsaT044640.1.exon3;Parent=MsaT044640.1
Chr8 CDS 62996001 62996396 62996001 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Chr8 exon 62996879 62997295 62996879 ID=MsaT044640.1.exon2;Parent=MsaT044640.1
Chr8 CDS 62996879 62997295 62996879 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Chr8 exon 62999138 62999167 62999138 ID=MsaT044640.1.exon1;Parent=MsaT044640.1
Chr8 CDS 62999138 62999167 62999138 ID=cds.MsaT044640.1;Parent=MsaT044640.1
Gene Sequence

>MsaT044640.1

ATGTCTATAATTCATATGAATAGCATAAAGGAACCAAATGATTACAGTTTCATCAACCAATCAAGCATAAACGCGGAAATTGTTGGTAGTTTAGAGAGTAACTTAGAAAGGCCTTCAAAATCACTCAAAACTACTACTACTTGCAGTTCAAGCTATGTCAAAACAGAACATCTCTCACAAAAAACAGCTCCATTTGTTCTTTCGTTTGAAAACGAGAATCCACCACCAATGAAGGTTGTACCAACCTTGAGGTCAAAAACCAAAGTTGTGAACTCAAAGAATGGACCAAGACGTGTAAAGAATCAGGAGAGCAACAAGAAGAACGGTTCATTTTCCAAATCTACAACTCATCACACACCAGATCATATAATTGCTGAGAGAATAAGGAGAGAAAAGATTAGCCAACAGTTCATAGCTCTTTCAGCCCTTATTCCAAACCTCAAAAAGATGGACAAGGCCTCGGTGTTGGGTGATGCTATAAAGTATGTGAAGGAGCTTAAGGAACAAGTAAAACTGTTGGAGGAGCAAAGCAAAAGCAAAAGTGTAGAACCGGTTACGGTTGTGAAGAAATTGTCTGAACTGTCTGCGGATGTCAATGTTTCGGACACTTCCTCAAATTCATGTAATGGAAACTCTGATGAAACATCAAAAACAAATCTGTCACTACCTGAAGTTAAAGCTAGTTTGTCGGGGAAGAATGTGCTGATTAGGATCCTCTGTGAGAAAGATAAGGCAGTGATGGTGAATGTATATAGAGAAATAGAGAAACTTCATCTCTTGGTTATCAATGCTAGTTCGTTTTCATTTGGTTCCTCTGCTCTGGCCATAACCATCATTGCCCAGATGGAGAATGAATTGAACATGAGCATCCAGAAACTGGCAAAAAAACTGAGAGTTGGACTGGGATTAATGGAGGACACTACTTTGTTTAATGATTATGAGTACCCTATGGATTCATCTGCATTTCAGTTTGATGATATGGCATACTTCAAGTCTTTCTCAGCATCCCCACAAGAGAGTACTTATTCCTCTCATACCAACAACATCAACAACAAGCGAATCCATTCTGAAAGCCCACAGAACAGTTCTTTCCCCATTGAAAGTAGTCCAGATCAATCTGTTGTCTCAGCAACACCGCCAACTAAAGTACTCAAGGCTTCTCCCAAAATCATTTCATTTGATAATTCTTCTCAACAATTTTACAACATGGATGCAAAGGTTAAGAAGCTTAAAACCGAGATCGGGTACGGAGAAAACTTGAATTTTGGAAGTGTGATATCTCAAGGTGATTATTATAAACGAGAGAACAAGGTTGCTGCTGTAAATAGAAACCCTATTCAGGCTCAAGATCATGTTATGGCCGAAAGAAGACGAAGGGAAAAGCTTAGCCAGAGATTCATTGCTCTTTCCTCCATTCTTCCTGGACTGAAAAAGATGGACAAGGCAACTATTTTGGAAGATGCTATTAAACATATGAAGCAACTAAAGGAACGAGTGAAGACATTAGAGGAACAAGTAGCCGACAAAAAGGTCGAATCAGCAGTCTTTGTGAAGAGATCTATTCTCTTCGACGATGATGGCAGCTCTTCTTGCGACGACAACTCAGACAAATCACTCCCAAAAATCGAAGCAAGAGTTTCTGGAAAAGACATGCTCATCAGAATCCACGGTGATAAACATTGCGGCCGATCTGCGGCCGCAATACTCAACTTATTGGAAAAGCATCATCTCACTGTTCAAAGTAGTTCTATTTTACCATTTGGAAATAACTATCTTGATATAACCATTGTTGCTCAGATGAATAAGGAATATTGCTTAACAATAAAGGATCTCGTAAGAAGCATAAGTCAGGTTTTGAGGCAGCATATTTGA
Protein sequence

>MsaT044640.1

MSIIHMNSIKEPNDYSFINQSSINAEIVGSLESNLERPSKSLKTTTTCSSSYVKTEHLSQKTAPFVLSFENENPPPMKVVPTLRSKTKVVNSKNGPRRVKNQESNKKNGSFSKSTTHHTPDHIIAERIRREKISQQFIALSALIPNLKKMDKASVLGDAIKYVKELKEQVKLLEEQSKSKSVEPVTVVKKLSELSADVNVSDTSSNSCNGNSDETSKTNLSLPEVKASLSGKNVLIRILCEKDKAVMVNVYREIEKLHLLVINASSFSFGSSALAITIIAQMENELNMSIQKLAKKLRVGLGLMEDTTLFNDYEYPMDSSAFQFDDMAYFKSFSASPQESTYSSHTNNINNKRIHSESPQNSSFPIESSPDQSVVSATPPTKVLKASPKIISFDNSSQQFYNMDAKVKKLKTEIGYGENLNFGSVISQGDYYKRENKVAAVNRNPIQAQDHVMAERRRREKLSQRFIALSSILPGLKKMDKATILEDAIKHMKQLKERVKTLEEQVADKKVESAVFVKRSILFDDDGSSSCDDNSDKSLPKIEARVSGKDMLIRIHGDKHCGRSAAAILNLLEKHHLTVQSSSILPFGNNYLDITIVAQMNKEYCLTIKDLVRSISQVLRQHI*