AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044641

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044641 MtrunA17_Chr4g0054581 92.167 383 27 1 1 383 1 380 0.0 655
MsaG044641 MtrunA17_Chr2g0307131 60.102 391 110 11 1 381 1 355 2.95e-135 390
MsaG044641 MtrunA17_Chr2g0307141 58.015 393 123 10 1 383 1 361 1.24e-125 366
MsaG044641 MtrunA17_Chr6g0468491 55.435 368 101 7 24 382 1 314 6.57e-122 355
MsaG044641 MtrunA17_Chr4g0033391 52.699 389 105 10 1 380 1 319 1.11e-114 337
MsaG044641 MtrunA17_Chr4g0054621 50.503 398 125 14 1 382 1 342 2.26e-108 322
MsaG044641 MtrunA17_Chr4g0033291 52.941 391 113 10 1 382 1 329 1.20e-106 317
MsaG044641 MtrunA17_Chr6g0468461 52.255 377 107 11 1 365 1 316 1.39e-106 317
MsaG044641 MtrunA17_Chr4g0033281 50.636 393 120 9 1 384 1 328 2.56e-104 311
MsaG044641 MtrunA17_Chr7g0225901 45.238 336 86 8 24 357 1 240 1.02e-72 227
MsaG044641 MtrunA17_Chr5g0400621 51.527 262 97 6 121 381 101 333 1.94e-69 221
MsaG044641 MtrunA17_Chr2g0280161 50.802 187 82 4 196 380 175 353 4.47e-55 185
MsaG044641 MtrunA17_Chr5g0400661 36.605 377 179 13 13 377 6 334 5.26e-55 184
MsaG044641 MtrunA17_Chr4g0002801 49.738 191 89 3 191 378 145 331 8.29e-54 181
MsaG044641 MtrunA17_Chr2g0333641 40.602 266 127 6 108 372 67 302 4.09e-50 171
MsaG044641 MtrunA17_Chr5g0400641 54.268 164 66 3 201 363 1 156 3.76e-49 163
MsaG044641 MtrunA17_Chr4g0002781 40.741 270 121 6 116 378 113 350 7.47e-49 168
MsaG044641 MtrunA17_Chr8g0336681 39.474 266 134 6 111 373 90 331 5.33e-47 163
MsaG044641 MtrunA17_Chr4g0049331 43.781 201 102 4 182 380 119 310 5.52e-46 160
MsaG044641 MtrunA17_Chr2g0333621 38.710 279 135 7 108 381 68 315 1.60e-44 156
MsaG044641 MtrunA17_Chr4g0054561 47.120 191 86 6 185 372 142 320 2.37e-44 156
MsaG044641 MtrunA17_Chr4g0054571 38.170 317 161 11 69 379 44 331 3.05e-44 155
MsaG044641 MtrunA17_Chr4g0054551 44.737 190 92 5 185 372 142 320 4.13e-44 155
MsaG044641 MtrunA17_Chr5g0400671 33.832 334 160 13 68 377 27 323 3.28e-39 142
MsaG044641 MtrunA17_Chr2g0333611 38.914 221 106 7 167 379 18 217 9.27e-39 138
MsaG044641 MtrunA17_Chr2g0333541 40.000 220 125 3 159 376 53 267 1.17e-38 139
MsaG044641 MtrunA17_Chr7g0249811 35.081 248 132 6 140 385 112 332 5.08e-36 134
MsaG044641 MtrunA17_Chr4g0002831 41.212 165 61 5 221 375 64 202 8.12e-28 108
MsaG044641 MtrunA17_Chr7g0233441 39.024 164 65 4 221 375 57 194 6.54e-26 103
MsaG044641 MtrunA17_Chr4g0002851 37.423 163 61 4 221 375 65 194 5.25e-24 98.2
MsaG044641 MtrunA17_Chr8g0366751 33.537 164 96 3 190 342 448 609 1.96e-17 84.0
MsaG044641 MtrunA17_Chr1g0185621 30.851 188 108 6 198 372 475 653 5.28e-17 82.8
MsaG044641 MtrunA17_Chr5g0411341 31.111 180 111 3 190 358 479 656 9.96e-16 79.0
MsaG044641 MtrunA17_Chr8g0346161 29.139 151 88 4 198 342 291 428 1.42e-15 78.2
MsaG044641 MtrunA17_Chr7g0275821 53.571 56 26 0 198 253 252 307 6.20e-14 72.8
MsaG044641 MtrunA17_Chr7g0260841 47.059 68 30 1 190 251 341 408 5.07e-13 70.5
MsaG044641 MtrunA17_Chr8g0365711 24.699 166 119 3 191 354 237 398 1.56e-12 68.6
MsaG044641 MtrunA17_Chr8g0346151 32.203 118 74 2 198 314 279 391 5.13e-12 67.0
MsaG044641 MtrunA17_Chr1g0211081 24.277 173 111 3 190 342 309 481 7.21e-12 66.6
MsaG044641 MtrunA17_Chr7g0270871 46.875 64 34 0 187 250 201 264 3.44e-11 64.3
MsaG044641 MtrunA17_Chr3g0131691 25.806 155 113 2 183 335 283 437 3.57e-11 64.7
MsaG044641 MtrunA17_Chr1g0153241 36.538 104 63 2 153 254 49 151 4.52e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044641 AT4G37850.2 38.095 294 141 10 101 380 62 328 6.73e-50 171
MsaG044641 AT4G37850.1 38.095 294 141 10 101 380 62 328 6.73e-50 171
MsaG044641 AT2G22750.3 37.008 381 181 14 7 379 3 332 3.15e-44 156
MsaG044641 AT2G22750.2 46.465 198 102 2 183 379 110 304 5.90e-44 155
MsaG044641 AT2G22750.5 46.465 198 102 2 183 379 134 328 9.87e-44 155
MsaG044641 AT2G22750.4 37.008 381 180 15 7 379 3 331 8.44e-43 152
MsaG044641 AT2G22750.1 46.465 198 101 3 183 379 110 303 1.59e-42 151
MsaG044641 AT2G22760.1 41.837 196 103 5 186 377 107 295 2.92e-40 144
MsaG044641 AT2G22760.2 41.143 175 93 4 186 357 107 274 7.26e-34 127
MsaG044641 AT2G22770.1 34.539 304 159 10 82 375 44 317 9.24e-31 120
MsaG044641 AT4G17880.1 31.461 178 102 3 190 358 402 568 1.31e-15 79.0
MsaG044641 AT1G32640.1 33.121 157 82 4 190 335 438 582 2.01e-15 78.6
MsaG044641 AT5G46760.1 27.041 196 115 5 190 361 401 592 3.33e-15 77.8
MsaG044641 AT2G46510.1 47.297 74 33 1 190 257 381 454 5.63e-15 77.0
MsaG044641 AT1G01260.1 47.143 70 37 0 192 261 427 496 1.44e-14 75.9
MsaG044641 AT1G01260.2 47.143 70 37 0 192 261 427 496 1.44e-14 75.9
MsaG044641 AT1G01260.3 47.143 70 37 0 192 261 427 496 1.44e-14 75.9
MsaG044641 AT4G16430.1 49.254 67 34 0 192 258 314 380 4.42e-14 73.9
MsaG044641 AT1G63650.3 29.605 152 89 4 198 335 405 552 3.17e-13 71.6
MsaG044641 AT1G63650.1 29.605 152 89 4 198 335 405 552 3.17e-13 71.6
MsaG044641 AT1G63650.2 29.605 152 89 4 198 335 405 552 3.17e-13 71.6
MsaG044641 AT4G09820.1 40.000 95 50 2 189 276 352 446 2.96e-12 68.6
MsaG044641 AT2G16910.1 28.144 167 94 4 199 345 315 475 4.80e-12 67.8
MsaG044641 AT4G00870.1 32.031 128 64 2 199 317 250 363 8.49e-12 66.6
MsaG044641 AT5G41315.1 25.126 199 123 6 192 370 435 627 1.48e-11 66.6
MsaG044641 AT5G41315.3 25.126 199 123 6 192 370 440 632 1.56e-11 66.2
MsaG044641 AT5G41315.2 25.126 199 123 6 192 370 443 635 1.58e-11 66.2
MsaG044641 AT5G57150.3 29.240 171 106 4 196 358 53 216 1.69e-11 63.9
MsaG044641 AT4G29930.3 28.090 178 103 6 199 357 55 226 2.03e-11 64.3
MsaG044641 AT4G29930.7 28.090 178 103 6 199 357 55 226 2.03e-11 64.3
MsaG044641 AT4G29930.1 28.333 180 104 6 199 359 55 228 2.08e-11 64.3
MsaG044641 AT5G57150.4 29.412 170 105 4 196 357 53 215 2.79e-11 63.9
MsaG044641 AT5G57150.1 29.651 172 106 4 196 359 52 216 2.86e-11 63.5
MsaG044641 AT5G57150.2 29.412 170 105 4 196 357 53 215 3.07e-11 63.2
MsaG044641 AT5G57150.5 29.412 170 105 4 196 357 52 214 3.14e-11 63.2
MsaG044641 AT3G06120.1 23.280 189 121 4 198 368 2 184 4.23e-11 62.4
MsaG044641 AT2G28160.2 27.381 168 109 3 177 343 115 270 6.34e-11 63.5
MsaG044641 AT2G28160.1 27.381 168 109 3 177 343 115 270 6.46e-11 63.5
MsaG044641 AT4G29930.6 25.926 162 105 3 199 359 55 202 8.42e-11 62.0
MsaG044641 AT4G29930.2 25.309 162 103 2 199 359 55 199 9.68e-11 61.6

Find 67 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAATCCCCTTGTGCTTC+TGG 0.171911 8:+63004116 None:intergenic
GTGTTGGTAGGAGGAGAATT+TGG 0.206656 8:+63004959 None:intergenic
CAGCAAATGGCTTTCTGATT+TGG 0.214444 8:-63005389 MsaT044641.1:intron
TAAAGAGCGTTTGGAAGTTT+TGG 0.276908 8:-63004335 MsaT044641.1:CDS
GACATGAATGTTTCTCATTT+GGG 0.281293 8:+63004862 None:intergenic
AGACATGAATGTTTCTCATT+TGG 0.284212 8:+63004861 None:intergenic
CATTAGCATCAAGGAAGTTT+AGG 0.295193 8:+63005239 None:intergenic
AAGTTTAGGTTGCATTCTTC+AGG 0.308908 8:+63005253 None:intergenic
CCAGAAGCACAAGGGGATTC+TGG 0.313026 8:-63004116 MsaT044641.1:CDS
TGTTGGTAGGAGGAGAATTT+GGG 0.316269 8:+63004960 None:intergenic
TCTTGTTCCTAACCTGAAAA+AGG 0.316557 8:-63004687 MsaT044641.1:intron
GTTTCTTCTGATACGTTCAT+TGG 0.319465 8:+63005412 None:intergenic
CAGGAGTGAGTCCACAAAAC+TGG 0.341350 8:+63004933 None:intergenic
ATAGCAGTGTCTTACCCTTT+GGG 0.356671 8:-63004048 MsaT044641.1:CDS
CAAATCAGAAAGCCATTTGC+TGG 0.362893 8:+63005390 None:intergenic
GGCGTTGGCTCGAGACCTTT+TGG 0.371700 8:+63004778 None:intergenic
GATGATGATGGAGAAAGTTT+TGG 0.378456 8:+63005025 None:intergenic
AATAGCAGTGTCTTACCCTT+TGG 0.385303 8:-63004049 MsaT044641.1:CDS
GCTTTCTTTCAGCCATGATA+TGG 0.385864 8:+63004750 None:intergenic
GAAAGAGCTTAAAGAGCGTT+TGG 0.403263 8:-63004344 MsaT044641.1:CDS
CTGCAAGAGCAATGAAGCTT+TGG 0.405262 8:+63004711 None:intergenic
CATCATCATCAATGATACTA+TGG 0.407350 8:+63004257 None:intergenic
GTCTCAACTCAAAATCCTAA+AGG 0.422120 8:-63004842 MsaT044641.1:CDS
GTTGGTGATAGTTGTGGAAT+TGG 0.425475 8:+63005162 None:intergenic
TGCTGTTGGGTTTGTTCTTG+TGG 0.426188 8:+63005202 None:intergenic
CAGGGAAAGAGATGCTGATT+CGG 0.434047 8:-63004147 MsaT044641.1:CDS
CAAGTGCAAGCAAAAGTGTC+AGG 0.441082 8:-63004166 MsaT044641.1:CDS
AGGAGTGAGTCCACAAAACT+GGG 0.451243 8:+63004934 None:intergenic
TCTCAACTCAAAATCCTAAA+GGG 0.458505 8:-63004841 MsaT044641.1:CDS
TGAACTCAAACATTGCTGTT+GGG 0.459835 8:+63005189 None:intergenic
CTGAACTCAAACATTGCTGT+TGG 0.465443 8:+63005188 None:intergenic
TCATATGATGAGAGTATTGA+AGG 0.466240 8:-63004208 MsaT044641.1:CDS
TGACATTACTATCATTGCTC+AGG 0.494765 8:-63004017 MsaT044641.1:intron
AGCAATGATAGTAATGTCAA+TGG 0.495292 8:+63004021 None:intergenic
TTTGACTTTGAGTTTAAGGC+AGG 0.501893 8:+63004914 None:intergenic
TTCAGCCATGATATGGTCAT+CGG 0.521311 8:+63004757 None:intergenic
CATGATATGGTCATCGGCGT+TGG 0.524246 8:+63004763 None:intergenic
AAACTTCCTTGATGCTAATG+AGG 0.524526 8:-63005236 MsaT044641.1:CDS
TCATCAATGATACTATGGTC+TGG 0.549545 8:+63004262 None:intergenic
ACTCTTCCTCATTAGCATCA+AGG 0.553454 8:+63005230 None:intergenic
TATCAGAAGAAACCAGCAAA+TGG 0.554188 8:-63005402 MsaT044641.1:CDS
TGAATGTTTCTCATTTGGGG+TGG 0.558418 8:+63004866 None:intergenic
CAACGCCGATGACCATATCA+TGG 0.570050 8:-63004762 MsaT044641.1:CDS
TGAAATTGAGAGACCAACAA+TGG 0.572716 8:-63005095 MsaT044641.1:CDS
AAAGATGAGTTAGATGATGA+TGG 0.577216 8:+63005013 None:intergenic
TAGCAGTGTCTTACCCTTTG+GGG 0.577291 8:-63004047 MsaT044641.1:CDS
TAAAATTTGGTAGATGGACA+AGG 0.590351 8:-63004398 MsaT044641.1:intron
ATGTCAATGGAATCCCCAAA+GGG 0.596532 8:+63004034 None:intergenic
AGGGTACAACTTGAGCATAA+AGG 0.600355 8:-63002759 MsaT044641.1:CDS
ACTTCAATGCTGCCATGCGT+AGG 0.601033 8:+63002723 None:intergenic
TGGTAGGAGGAGAATTTGGG+AGG 0.603292 8:+63004963 None:intergenic
GAATGAGTTGGTGATAGTTG+TGG 0.603767 8:+63005156 None:intergenic
GGGGATTCTGGTGAAAGTCA+TGG 0.606251 8:-63004104 MsaT044641.1:CDS
GATTCACTGCCAGAAGCACA+AGG 0.606463 8:-63004125 MsaT044641.1:CDS
TTCACTGCCAGAAGCACAAG+GGG 0.606586 8:-63004123 MsaT044641.1:CDS
AATGTCAATGGAATCCCCAA+AGG 0.614554 8:+63004033 None:intergenic
TGTTTCATCAGTGAATGAGT+TGG 0.619624 8:+63005144 None:intergenic
ACATGAATGTTTCTCATTTG+GGG 0.622328 8:+63004863 None:intergenic
AAGTGCAAGCAAAAGTGTCA+GGG 0.633121 8:-63004165 MsaT044641.1:CDS
GAAAACAAAGGTAGAGTCCG+TGG 0.640082 8:-63004299 MsaT044641.1:CDS
GAGTGAGTCCACAAAACTGG+GGG 0.649560 8:+63004936 None:intergenic
TGGTTTCTTCAGAACAACCA+CGG 0.659948 8:+63004282 None:intergenic
GGAGTGAGTCCACAAAACTG+GGG 0.660208 8:+63004935 None:intergenic
ATTCACTGCCAGAAGCACAA+GGG 0.669813 8:-63004124 MsaT044641.1:CDS
AGAACAAAACAAGAAAACAA+AGG 0.679620 8:-63004311 MsaT044641.1:CDS
ACTTGTCAAGAACCTACGCA+TGG 0.686710 8:-63002735 MsaT044641.1:CDS
AGTGAGTCCACAAAACTGGG+GGG 0.773710 8:+63004937 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 63002700 63005455 63002700 ID=MsaG044641
Chr8 mRNA 63002700 63005455 63002700 ID=MsaT044641.1;Parent=MsaG044641
Chr8 exon 63002700 63002789 63002700 ID=MsaT044641.1.exon4;Parent=MsaT044641.1
Chr8 CDS 63002700 63002789 63002700 ID=cds.MsaT044641.1;Parent=MsaT044641.1
Chr8 exon 63004018 63004407 63004018 ID=MsaT044641.1.exon3;Parent=MsaT044641.1
Chr8 CDS 63004018 63004407 63004018 ID=cds.MsaT044641.1;Parent=MsaT044641.1
Chr8 exon 63004688 63005299 63004688 ID=MsaT044641.1.exon2;Parent=MsaT044641.1
Chr8 CDS 63004688 63005299 63004688 ID=cds.MsaT044641.1;Parent=MsaT044641.1
Chr8 exon 63005390 63005455 63005390 ID=MsaT044641.1.exon1;Parent=MsaT044641.1
Chr8 CDS 63005390 63005455 63005390 ID=cds.MsaT044641.1;Parent=MsaT044641.1
Gene Sequence

>MsaT044641.1

ATGGAAGAAATCAACAACACACCAATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCCACAAGAACAAACCCAACAGCAATGTTTGAGTTCAGAGTCCAATTCCACAACTATCACCAACTCATTCACTGATGAAACAACTTTAGACTCTTTTGACTTTGGTTTTGAAATTGAGAGACCAACAATGGAGCTGAAAACAATTGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTTGTCTTTTGACAACCTCCCAAATTCTCCTCCTACCAACACCCCCCAGTTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAAACAGAACAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAGAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCTTACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA
Protein sequence

>MsaT044641.1

MEEINNTPMNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLPQEQTQQQCLSSESNSTTITNSFTDETTLDSFDFGFEIERPTMELKTIVSDNNIIETISPKLSPSSSNSSLHSQILSFDNLPNSPPTNTPQFCGLTPALNSKSKQNKTVSVFPPQMRNIHVSTQNPKGLSKTQNFATKTSQTKRSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKERLEVLEEQNKKTKVESVVVLKKPDHSIIDDDDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSLQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE*