AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044649

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044649 MtrunA17_Chr4g0054701 99.213 127 1 0 3 129 28 154 4.49e-89 255
MsaG044649 MtrunA17_Chr8g0381011 73.451 113 30 0 6 118 2 114 5.66e-58 177
MsaG044649 MtrunA17_Chr2g0303181 60.526 114 42 2 6 116 2 115 1.84e-47 150
MsaG044649 MtrunA17_Chr4g0047341 56.000 75 33 0 45 119 40 114 1.87e-24 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044649 AT1G32540.1 94.215 121 7 0 5 125 64 184 9.02e-80 233
MsaG044649 AT1G32540.3 94.215 121 7 0 5 125 31 151 4.66e-79 230
MsaG044649 AT1G32540.2 94.215 121 7 0 5 125 31 151 4.66e-79 230
MsaG044649 AT1G32540.5 92.500 120 9 0 6 125 32 151 3.08e-77 226
MsaG044649 AT1G32540.7 97.368 76 2 0 5 80 64 139 1.07e-48 153
MsaG044649 AT1G32540.4 100.000 74 0 0 6 79 32 105 2.38e-48 151
MsaG044649 AT1G32540.4 48.529 68 34 1 46 112 33 100 1.25e-11 57.8
MsaG044649 AT1G32540.6 100.000 74 0 0 6 79 32 105 2.38e-48 151
MsaG044649 AT1G32540.6 48.529 68 34 1 46 112 33 100 1.25e-11 57.8
MsaG044649 AT4G20380.2 60.484 124 38 3 6 118 7 130 8.41e-41 134
MsaG044649 AT4G20380.2 45.455 77 39 1 47 120 9 85 2.84e-15 68.9
MsaG044649 AT4G20380.7 60.484 124 38 3 6 118 7 130 8.41e-41 134
MsaG044649 AT4G20380.7 45.455 77 39 1 47 120 9 85 2.84e-15 68.9
MsaG044649 AT4G20380.6 60.484 124 38 3 6 118 2 125 9.57e-41 134
MsaG044649 AT4G20380.6 45.455 77 39 1 47 120 4 80 2.79e-15 68.9
MsaG044649 AT4G20380.3 60.484 124 38 3 6 118 2 125 9.57e-41 134
MsaG044649 AT4G20380.3 45.455 77 39 1 47 120 4 80 2.79e-15 68.9
MsaG044649 AT4G20380.4 60.484 124 38 3 6 118 2 125 9.57e-41 134
MsaG044649 AT4G20380.4 45.455 77 39 1 47 120 4 80 2.79e-15 68.9
MsaG044649 AT4G20380.1 60.484 124 38 3 6 118 2 125 9.57e-41 134
MsaG044649 AT4G20380.1 45.455 77 39 1 47 120 4 80 2.79e-15 68.9
MsaG044649 AT4G20380.5 60.484 124 38 3 6 118 2 125 9.57e-41 134
MsaG044649 AT4G20380.5 45.455 77 39 1 47 120 4 80 2.79e-15 68.9
MsaG044649 AT4G20380.9 60.656 122 37 3 6 116 2 123 1.71e-40 132
MsaG044649 AT4G20380.9 45.455 77 39 1 47 120 4 80 2.40e-15 67.8
MsaG044649 AT4G20380.8 50.667 150 37 3 6 118 2 151 1.46e-37 127
MsaG044649 AT4G20380.8 46.154 78 39 1 47 121 4 81 1.67e-15 70.1
MsaG044649 AT4G21610.1 55.294 85 35 1 44 125 57 141 5.78e-28 100
MsaG044649 AT4G21610.2 60.274 73 29 0 44 116 57 129 8.83e-27 97.4

Find 25 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTGCCACTTAATGTTAAA+TGG 0.249121 8:-63178901 None:intergenic
CAGAGTCAACTTGTGTGTTC+TGG 0.355331 8:-63178803 MsaT044649.1:CDS
ATGCTACTTATGTATCAATA+TGG 0.369271 8:-63178260 MsaT044649.1:CDS
ATTGCAAACTGCACATTTCA+CGG 0.413666 8:+63178229 None:intergenic
GTGTATTGTGACAGCAAATC+AGG 0.446436 8:-63178312 MsaT044649.1:intron
CATACAGAAGTGGCTCCAAC+TGG 0.466065 8:+63178758 None:intergenic
GGATGTACATGAGTAAAGTA+TGG 0.469461 8:+63178536 None:intergenic
GTCACAGCAGTGCCACCTCC+TGG 0.473850 8:-63178716 MsaT044649.1:intron
GAAATGGCTCAGTTAGTTTG+TGG 0.475051 8:-63178565 MsaT044649.1:CDS
TGTATTCACAGGCACAGAAA+TGG 0.481941 8:-63178581 MsaT044649.1:intron
TCACACAGTCAATCTAGCTC+TGG 0.518089 8:-63178482 MsaT044649.1:CDS
AACCTTCTTATGTATCCAGT+TGG 0.523909 8:-63178773 MsaT044649.1:CDS
CTCCAACTGGATACATAAGA+AGG 0.572621 8:+63178771 None:intergenic
CTGCTCCGTCGTGCTTGCTG+AGG 0.583212 8:+63178026 None:intergenic
TATTTCATCATACCAGGAGG+TGG 0.599736 8:+63178704 None:intergenic
AACAGCACAGCATACAGAAG+TGG 0.604154 8:+63178748 None:intergenic
ATGGCTCAGTTAGTTTGTGG+AGG 0.620989 8:-63178562 MsaT044649.1:CDS
ACAGTCAATCTAGCTCTGGA+AGG 0.636792 8:-63178478 MsaT044649.1:intron
ATGTCAACTGTGGTAACTGC+AGG 0.637282 8:-63178283 MsaT044649.1:CDS
AACTATACTAACCCCAACTG+AGG 0.647011 8:+63178199 None:intergenic
GCAGGCCTCAGCAAGCACGA+CGG 0.676096 8:-63178031 MsaT044649.1:intron
ATTGAACACTCGTCGCTCCG+CGG 0.692743 8:+63178515 None:intergenic
ACTTTACTCATGTACATCCG+CGG 0.709515 8:-63178532 MsaT044649.1:CDS
CAGGTGGCACATGTCAACTG+TGG 0.714575 8:-63178293 MsaT044649.1:CDS
TATTGTGACAGCAAATCAGG+TGG 0.775426 8:-63178309 MsaT044649.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 63178008 63178911 63178008 ID=MsaG044649
Chr8 mRNA 63178008 63178911 63178008 ID=MsaT044649.1;Parent=MsaG044649
Chr8 exon 63178008 63178049 63178008 ID=MsaT044649.1.exon5;Parent=MsaT044649.1
Chr8 CDS 63178008 63178049 63178008 ID=cds.MsaT044649.1;Parent=MsaT044649.1
Chr8 exon 63178211 63178320 63178211 ID=MsaT044649.1.exon4;Parent=MsaT044649.1
Chr8 CDS 63178211 63178320 63178211 ID=cds.MsaT044649.1;Parent=MsaT044649.1
Chr8 exon 63178479 63178592 63178479 ID=MsaT044649.1.exon3;Parent=MsaT044649.1
Chr8 CDS 63178479 63178592 63178479 ID=cds.MsaT044649.1;Parent=MsaT044649.1
Chr8 exon 63178717 63178830 63178717 ID=MsaT044649.1.exon2;Parent=MsaT044649.1
Chr8 CDS 63178717 63178830 63178717 ID=cds.MsaT044649.1;Parent=MsaT044649.1
Chr8 exon 63178902 63178911 63178902 ID=MsaT044649.1.exon1;Parent=MsaT044649.1
Chr8 CDS 63178902 63178911 63178902 ID=cds.MsaT044649.1;Parent=MsaT044649.1
Gene Sequence

>MsaT044649.1

ATGTTAAATGGTGCACAGAGTCAACTTGTGTGTTCTGGTTGTAGAAACCTTCTTATGTATCCAGTTGGAGCCACTTCTGTATGCTGTGCTGTTTGCAATGCTGTCACAGCAGTGCCACCTCCTGGCACAGAAATGGCTCAGTTAGTTTGTGGAGGATGCCATACTTTACTCATGTACATCCGCGGAGCGACGAGTGTTCAATGCTCTTGTTGTCACACAGTCAATCTAGCTCTGGAAGCAAATCAGGTGGCACATGTCAACTGTGGTAACTGCAGGATGCTACTTATGTATCAATATGGAGCAAGATCCGTGAAATGTGCAGTTTGCAATTTTGTTACCTCAGTTGGGGCCTCAGCAAGCACGACGGAGCAGCAGAAGTTCAGCACCTAA
Protein sequence

>MsaT044649.1

MLNGAQSQLVCSGCRNLLMYPVGATSVCCAVCNAVTAVPPPGTEMAQLVCGGCHTLLMYIRGATSVQCSCCHTVNLALEANQVAHVNCGNCRMLLMYQYGARSVKCAVCNFVTSVGASASTTEQQKFST*