AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044650 MtrunA17_Chr1g0203051 55.963 218 86 2 1 216 1 210 3.98e-74 240
MsaG044650 MtrunA17_Chr1g0203051 57.143 63 27 0 233 295 411 473 6.88e-19 87.4
MsaG044650 MtrunA17_Chr8g0389811 47.101 138 71 1 61 196 58 195 1.51e-44 151
MsaG044650 MtrunA17_Chr2g0331371 40.625 128 74 1 68 193 36 163 1.23e-28 110
MsaG044650 MtrunA17_Chr1g0171741 33.493 209 130 4 11 215 59 262 1.70e-28 110
MsaG044650 MtrunA17_Chr6g0473421 42.105 95 53 1 68 160 36 130 9.45e-24 95.5
MsaG044650 MtrunA17_Chr2g0287651 30.247 162 111 1 70 229 58 219 1.60e-20 92.4
MsaG044650 MtrunA17_Chr1g0172931 41.818 110 62 1 60 167 49 158 7.94e-20 84.7
MsaG044650 MtrunA17_Chr2g0308841 52.542 59 28 0 233 291 4 62 4.00e-18 82.8
MsaG044650 MtrunA17_Chr6g0458391 29.167 192 130 6 15 204 20 207 1.03e-14 75.1
MsaG044650 MtrunA17_Chr2g0276841 30.282 142 92 4 93 228 76 216 5.80e-13 69.7
MsaG044650 MtrunA17_Chr1g0198761 34.109 129 73 5 99 216 59 186 9.71e-13 68.9
MsaG044650 MtrunA17_Chr7g0228051 51.471 68 30 1 32 96 18 85 2.71e-12 62.0
MsaG044650 MtrunA17_Chr2g0290391 29.323 133 91 2 99 228 63 195 3.52e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044650 AT5G28530.5 31.200 125 81 3 99 219 67 190 2.54e-14 74.3
MsaG044650 AT5G28530.4 31.200 125 81 3 99 219 67 190 2.77e-14 73.9
MsaG044650 AT5G28530.1 31.200 125 81 3 99 219 67 190 2.99e-14 73.9
MsaG044650 AT5G28530.3 31.200 125 81 3 99 219 67 190 3.12e-14 73.9
MsaG044650 AT5G28530.2 31.200 125 81 3 99 219 67 190 3.12e-14 73.9
MsaG044650 AT3G59470.3 30.058 173 103 6 70 227 47 216 4.35e-13 68.6
MsaG044650 AT3G59470.1 30.058 173 103 6 70 227 47 216 4.35e-13 68.6
MsaG044650 AT3G59470.2 30.058 173 103 6 70 227 59 228 4.65e-13 68.6
MsaG044650 AT1G10240.2 31.200 125 79 4 98 216 59 182 1.51e-12 68.9
MsaG044650 AT1G10240.1 31.200 125 79 4 98 216 59 182 1.51e-12 68.9
MsaG044650 AT3G06250.2 43.636 55 31 0 233 287 485 539 2.66e-11 65.1
MsaG044650 AT3G06250.1 43.636 55 31 0 233 287 485 539 2.66e-11 65.1
MsaG044650 AT3G06250.3 43.636 55 31 0 233 287 485 539 2.66e-11 65.1

Find 71 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAAATCTACCACGGATA+TGG 0.198700 8:-63189369 None:intergenic
CAACTAAACGATCCTGAAAA+TGG 0.285819 8:-63188265 None:intergenic
CCTCTGAACCTTCCGCATTA+AGG 0.302211 8:-63187943 None:intergenic
GATTTGTTGGTTGCAGAGTT+TGG 0.306543 8:+63189286 MsaT044650.1:CDS
CTGATACTTCCCATCCAAAA+AGG 0.344955 8:-63188552 None:intergenic
CCCAATGTAAGCGACATAAC+TGG 0.364706 8:+63187911 MsaT044650.1:CDS
ATTCCTACGTCACGCATCTA+TGG 0.368017 8:+63188646 MsaT044650.1:CDS
ATTTGTTGGTTGCAGAGTTT+GGG 0.383219 8:+63189287 MsaT044650.1:CDS
TTTGTCATAGAGAAGGTTAC+AGG 0.397074 8:+63188390 MsaT044650.1:CDS
CCCAGTTATGTCGCTTACAT+TGG 0.430950 8:-63187912 None:intergenic
TGAAACTATGTGGGATTTGT+TGG 0.439762 8:+63189273 MsaT044650.1:CDS
AAAGCCGTGTTGTTAAGAAC+AGG 0.447436 8:+63188339 MsaT044650.1:CDS
AAGTTCAAACGGTGTATGTT+CGG 0.449433 8:+63189229 MsaT044650.1:intron
GACATGTACAGTTCGTTGTA+TGG 0.455744 8:+63188149 MsaT044650.1:CDS
ATAAAATCTACCACGGATAT+GGG 0.460719 8:-63189368 None:intergenic
CCAGTTATGTCGCTTACATT+GGG 0.461886 8:-63187911 None:intergenic
TTGGGTTGGAAAATAATAAG+TGG 0.477062 8:+63189305 MsaT044650.1:CDS
CCAATGTAAGCGACATAACT+GGG 0.477737 8:+63187912 MsaT044650.1:CDS
GAGAGCAGAAGGCAACTAGT+AGG 0.491224 8:+63188441 MsaT044650.1:CDS
TTGAAGAGTTTGAAACTATG+TGG 0.492334 8:+63189263 MsaT044650.1:CDS
AACTAGTAGGTGTGGTTGCA+TGG 0.492607 8:+63188454 MsaT044650.1:CDS
CATGGACAATAGCTCTTGTT+CGG 0.498690 8:+63188091 MsaT044650.1:CDS
CATAGAGAAGGTTACAGGGT+TGG 0.507010 8:+63188395 MsaT044650.1:CDS
TGAAGAGTTTGAAACTATGT+GGG 0.510185 8:+63189264 MsaT044650.1:CDS
AATGAAAATGATGAGAAAGC+TGG 0.519700 8:+63188622 MsaT044650.1:CDS
TCGTGAGCTGTATGAGAAGC+GGG 0.523534 8:+63189330 MsaT044650.1:CDS
CCTTAAAATTAATTCTGTCA+AGG 0.525060 8:-63188214 None:intergenic
AATTTGTCCCGAAGTTCAAA+CGG 0.526146 8:+63189218 MsaT044650.1:intron
TGCGGAAGGTTCAGAGGTAG+AGG 0.537083 8:+63187949 MsaT044650.1:CDS
ACCTTTGTTTGTCATAGAGA+AGG 0.542041 8:+63188383 MsaT044650.1:CDS
GTGCAACTTTGACGATGAAA+CGG 0.547511 8:+63188172 MsaT044650.1:CDS
TCTGTCACTGAACCGACCAC+GGG 0.550131 8:-63189944 None:intergenic
ACATGTACAGTTCGTTGTAT+GGG 0.550841 8:+63188150 MsaT044650.1:CDS
TCGCTCATATTCCTATGTGC+AGG 0.551020 8:-63188584 None:intergenic
TTCTGTCACTGAACCGACCA+CGG 0.554234 8:-63189945 None:intergenic
CGATAGGAAAAGAGAGCAGA+AGG 0.554726 8:+63188430 MsaT044650.1:CDS
GTTGGTTGCAGAGTTTGGGT+TGG 0.554814 8:+63189291 MsaT044650.1:CDS
AATTTGTACGGTTGTGTACG+TGG 0.562187 8:+63188302 MsaT044650.1:CDS
CCGCAACGGCTTAAGAAAAG+AGG 0.575682 8:+63189983 MsaT044650.1:CDS
GGAGAGTGATGTATGTTCCT+CGG 0.577170 8:+63188112 MsaT044650.1:CDS
TGAAACGGCGGTTGATTGCA+TGG 0.577332 8:+63188187 MsaT044650.1:CDS
TGTATGAGAAGCGGGAGCAA+TGG 0.585526 8:+63189338 MsaT044650.1:CDS
CAGAAGGCAACTAGTAGGTG+TGG 0.587238 8:+63188446 MsaT044650.1:CDS
GTACACGTTGACGCTGGTAG+CGG 0.591900 8:+63188488 MsaT044650.1:CDS
TTGACGCTGGTAGCGGTCGA+TGG 0.592302 8:+63188495 MsaT044650.1:CDS
CCTTAATGCGGAAGGTTCAG+AGG 0.595778 8:+63187943 MsaT044650.1:CDS
TTCGTGAGCTGTATGAGAAG+CGG 0.599823 8:+63189329 MsaT044650.1:CDS
CGTGTTGTTAAGAACAGGGA+TGG 0.599865 8:+63188344 MsaT044650.1:CDS
TTGTCATAGAGAAGGTTACA+GGG 0.601328 8:+63188391 MsaT044650.1:CDS
GTTGATGAATTGCCCGTGGT+CGG 0.602393 8:+63189932 MsaT044650.1:CDS
CATCCCTGTTCTTAACAACA+CGG 0.608258 8:-63188343 None:intergenic
CAACGGCTTAAGAAAAGAGG+TGG 0.608660 8:+63189986 MsaT044650.1:CDS
GGATGCAACCTTAATGCGGA+AGG 0.614546 8:+63187935 MsaT044650.1:CDS
AGGGAATGCTACCTGCACAT+AGG 0.615340 8:+63188573 MsaT044650.1:CDS
AAAGTTTAGGTAATCCGCAA+CGG 0.615547 8:+63189969 MsaT044650.1:CDS
ACCTTCTCTATGACAAACAA+AGG 0.615884 8:-63188384 None:intergenic
TTGGAAGTAATGCTGCCGAT+AGG 0.626637 8:+63188414 MsaT044650.1:CDS
TACATTACGATGAAGACGCA+TGG 0.635500 8:-63190016 None:intergenic
AATCCATAGATGCGTGACGT+AGG 0.635928 8:-63188649 None:intergenic
TGGTCAACTGCCCATATCCG+TGG 0.650014 8:+63189358 MsaT044650.1:CDS
GTCTTCGACATCTTCATCCG+AGG 0.652680 8:-63188129 None:intergenic
CTGGTTTCAGAACAACCTCA+AGG 0.660159 8:+63189392 MsaT044650.1:CDS
ATTCAAGTACACGTTGACGC+TGG 0.666323 8:+63188482 MsaT044650.1:CDS
AACCAGCATAAAATCTACCA+CGG 0.675988 8:-63189375 None:intergenic
TTAAGAAAAGAGGTGGAGAG+CGG 0.677086 8:+63189993 MsaT044650.1:CDS
CAATGTAAGCGACATAACTG+GGG 0.679402 8:+63187913 MsaT044650.1:CDS
AAGCCGTGTTGTTAAGAACA+GGG 0.681295 8:+63188340 MsaT044650.1:CDS
TGAAGTTGATGAATTGCCCG+TGG 0.692394 8:+63189928 MsaT044650.1:CDS
CAACTTTGACGATGAAACGG+CGG 0.704083 8:+63188175 MsaT044650.1:CDS
AATGTAAGCGACATAACTGG+GGG 0.709527 8:+63187914 MsaT044650.1:CDS
TAGGGTCGGATGAAATCATG+CGG 0.716997 8:+63188240 MsaT044650.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 63187875 63190039 63187875 ID=MsaG044650
Chr8 mRNA 63187875 63190039 63187875 ID=MsaT044650.1;Parent=MsaG044650
Chr8 exon 63187875 63187970 63187875 ID=MsaT044650.1.exon1;Parent=MsaT044650.1
Chr8 CDS 63187875 63187970 63187875 ID=cds.MsaT044650.1;Parent=MsaT044650.1
Chr8 exon 63188089 63188688 63188089 ID=MsaT044650.1.exon2;Parent=MsaT044650.1
Chr8 CDS 63188089 63188688 63188089 ID=cds.MsaT044650.1;Parent=MsaT044650.1
Chr8 exon 63189232 63189413 63189232 ID=MsaT044650.1.exon3;Parent=MsaT044650.1
Chr8 CDS 63189232 63189413 63189232 ID=cds.MsaT044650.1;Parent=MsaT044650.1
Chr8 exon 63189925 63190039 63189925 ID=MsaT044650.1.exon4;Parent=MsaT044650.1
Chr8 CDS 63189925 63190039 63189925 ID=cds.MsaT044650.1;Parent=MsaT044650.1
Gene Sequence

>MsaT044650.1

ATGGATATTCATGAAATTCTAGAAATTTTCGGAATTCCCAATGTAAGCGACATAACTGGGGGATGCAACCTTAATGCGGAAGGTTCAGAGGTAGAGGACATGGACAATAGCTCTTGTTCGGAGAGTGATGTATGTTCCTCGGATGAAGATGTCGAAGACATGTACAGTTCGTTGTATGGGTGCAACTTTGACGATGAAACGGCGGTTGATTGCATGGCTGACCTTGACAGAATTAATTTTAAGGAAATAGGGTCGGATGAAATCATGCGGTACCATTTTCAGGATCGTTTAGTTGCATTCAAGTTTTACAATTTGTACGGTTGTGTACGTGGATTTTCCGGTAGAAAAAGCCGTGTTGTTAAGAACAGGGATGGCGATGTTGTTCAACAAACCTTTGTTTGTCATAGAGAAGGTTACAGGGTTGGAAGTAATGCTGCCGATAGGAAAAGAGAGCAGAAGGCAACTAGTAGGTGTGGTTGCATGGCAAAAATTCAAGTACACGTTGACGCTGGTAGCGGTCGATGGTACATAAAATCTGTTGACGATATTCACAATCACACCTTTTTGGATGGGAAGTATCAGGGAATGCTACCTGCACATAGGAATATGAGCGATTATGACAAATACCAAATGAAAATGATGAGAAAGCTGGCATTCCTACGTCACGCATCTATGGATTTTTTGCTTCACAGGCTGTTCAAACGGTGTATGTTCGGTGATTTTGATATTGAAGAGTTTGAAACTATGTGGGATTTGTTGGTTGCAGAGTTTGGGTTGGAAAATAATAAGTGGATTCGTGAGCTGTATGAGAAGCGGGAGCAATGGTCAACTGCCCATATCCGTGGTAGATTTTATGCTGGTTTCAGAACAACCTCAAGGGGTGAAGTTGATGAATTGCCCGTGGTCGGTTCAGTGACAGAAAAAAGTTTAGGTAATCCGCAACGGCTTAAGAAAAGAGGTGGAGAGCGGCCATGCGTCTTCATCGTAATGTAA
Protein sequence

>MsaT044650.1

MDIHEILEIFGIPNVSDITGGCNLNAEGSEVEDMDNSSCSESDVCSSDEDVEDMYSSLYGCNFDDETAVDCMADLDRINFKEIGSDEIMRYHFQDRLVAFKFYNLYGCVRGFSGRKSRVVKNRDGDVVQQTFVCHREGYRVGSNAADRKREQKATSRCGCMAKIQVHVDAGSGRWYIKSVDDIHNHTFLDGKYQGMLPAHRNMSDYDKYQMKMMRKLAFLRHASMDFLLHRLFKRCMFGDFDIEEFETMWDLLVAEFGLENNKWIRELYEKREQWSTAHIRGRFYAGFRTTSRGEVDELPVVGSVTEKSLGNPQRLKKRGGERPCVFIVM*