Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044697 | KEH26852.1 | 52.941 | 68 | 32 | 0 | 15 | 82 | 77 | 144 | 1.12e-13 | 75.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044697 | tr|A0A072UB72|A0A072UB72_MEDTR | 52.941 | 68 | 32 | 0 | 15 | 82 | 77 | 144 | 5.35e-14 | 75.5 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG044697 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAACTTATCACTTCAAAT+AGG | 0.238418 | 8:-63653009 | MsaT044697.1:CDS |
| TCACCTAAGTATTTCTCATT+TGG | 0.308885 | 8:+63652983 | None:intergenic |
| CAAACTTATCACTTCAAATA+GGG | 0.313955 | 8:-63653008 | MsaT044697.1:CDS |
| TCTACGGTCCTTCACGAACT+TGG | 0.378944 | 8:+63652952 | None:intergenic |
| ACAGGAGAGCATTACTTTGC+AGG | 0.412579 | 8:-63653059 | MsaT044697.1:CDS |
| TAAGTTTGCACTCATATTCT+CGG | 0.417716 | 8:+63653023 | None:intergenic |
| ACCTCCACAAAGAGACGATT+GGG | 0.451863 | 8:+63652887 | None:intergenic |
| AGAAATACTTAGGTGAAGAT+TGG | 0.462766 | 8:-63652976 | MsaT044697.1:CDS |
| TCCTCCCAATCGTCTCTTTG+TGG | 0.465471 | 8:-63652891 | MsaT044697.1:CDS |
| AGGTTGTCGACGTCGTCGTT+AGG | 0.468959 | 8:-63652868 | MsaT044697.1:CDS |
| TATCACCTTTCCTTAGTCTA+CGG | 0.505505 | 8:+63652936 | None:intergenic |
| GGTCCAAATGAGAAATACTT+AGG | 0.515401 | 8:-63652986 | MsaT044697.1:CDS |
| TCGTGAAGGACCGTAGACTA+AGG | 0.541590 | 8:-63652946 | MsaT044697.1:CDS |
| AACCTCCACAAAGAGACGAT+TGG | 0.547571 | 8:+63652886 | None:intergenic |
| CAACAAGAGATGTCAAGTAA+TGG | 0.551729 | 8:-63653214 | None:intergenic |
| CTACGGTCCTTCACGAACTT+GGG | 0.555110 | 8:+63652953 | None:intergenic |
| TCCCAATCGTCTCTTTGTGG+AGG | 0.560244 | 8:-63652888 | MsaT044697.1:CDS |
| TCTATCAGCCTTGCAGACAC+AGG | 0.595045 | 8:-63653077 | MsaT044697.1:CDS |
| AAACTTATCACTTCAAATAG+GGG | 0.599416 | 8:-63653007 | MsaT044697.1:CDS |
| ATTTGTTTAGCACTTCCGAT+AGG | 0.611879 | 8:-63653103 | MsaT044697.1:intron |
| AATGCTCTCCTGTGTCTGCA+AGG | 0.616315 | 8:+63653069 | None:intergenic |
| AAGGCTGATAGATATCCTAT+CGG | 0.658005 | 8:+63653088 | None:intergenic |
| TCCACAAAGAGACGATTGGG+AGG | 0.661851 | 8:+63652890 | None:intergenic |
| AAGGACCGTAGACTAAGGAA+AGG | 0.676015 | 8:-63652941 | MsaT044697.1:CDS |
| AGATTGGCCCAAGTTCGTGA+AGG | 0.684958 | 8:-63652960 | MsaT044697.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 63652859 | 63653227 | 63652859 | ID=MsaG044697 |
| Chr8 | mRNA | 63652859 | 63653227 | 63652859 | ID=MsaT044697.1;Parent=MsaG044697 |
| Chr8 | exon | 63652859 | 63653115 | 63652859 | ID=MsaT044697.1.exon2;Parent=MsaT044697.1 |
| Chr8 | CDS | 63652859 | 63653115 | 63652859 | ID=cds.MsaT044697.1;Parent=MsaT044697.1 |
| Chr8 | exon | 63653215 | 63653227 | 63653215 | ID=MsaT044697.1.exon1;Parent=MsaT044697.1 |
| Chr8 | CDS | 63653215 | 63653227 | 63653215 | ID=cds.MsaT044697.1;Parent=MsaT044697.1 |
| Gene Sequence |
| Protein sequence |
