AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044701

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044701 MtrunA17_Chr8g0374601 78.261 69 15 0 1 69 19 87 1.92e-33 116
MsaG044701 MtrunA17_Chr8g0374591 65.217 69 24 0 1 69 1 69 4.74e-26 93.2
MsaG044701 MtrunA17_Chr8g0374581 54.054 74 33 1 1 73 1 74 3.87e-20 80.5
MsaG044701 MtrunA17_Chr3g0097221 54.098 61 28 0 9 69 8 68 2.17e-17 73.9
MsaG044701 MtrunA17_Chr5g0402611 44.928 69 38 0 1 69 1 69 5.68e-16 70.5
MsaG044701 MtrunA17_Chr3g0115431 47.692 65 34 0 9 73 10 74 2.24e-15 67.8
MsaG044701 MtrunA17_Chr5g0437571 49.180 61 31 0 9 69 7 67 1.03e-13 62.4
MsaG044701 MtrunA17_Chr4g0055441 44.444 63 34 1 8 69 2 64 1.34e-13 62.0
MsaG044701 MtrunA17_Chr5g0444351 39.326 89 39 1 5 78 2 90 1.46e-13 61.6
MsaG044701 MtrunA17_Chr8g0353981 45.902 61 33 0 9 69 8 68 2.15e-13 62.8
MsaG044701 MtrunA17_Chr7g0227511 45.902 61 33 0 9 69 12 72 2.31e-13 61.6
MsaG044701 MtrunA17_Chr4g0058651 44.615 65 36 0 9 73 12 76 2.40e-13 62.0
MsaG044701 MtrunA17_Chr3g0106101 44.262 61 34 0 9 69 8 68 3.72e-13 61.2
MsaG044701 MtrunA17_Chr3g0117121 45.902 61 33 0 9 69 11 71 6.44e-13 60.8
MsaG044701 MtrunA17_Chr3g0114061 42.029 69 40 0 1 69 1 69 6.97e-13 60.1
MsaG044701 MtrunA17_Chr6g0449651 41.538 65 38 0 9 73 68 132 2.05e-12 59.7
MsaG044701 MtrunA17_Chr6g0460941 43.548 62 34 1 9 69 8 69 2.35e-12 57.4
MsaG044701 MtrunA17_Chr7g0225111 42.188 64 37 0 5 68 2 65 3.28e-12 57.4
MsaG044701 MtrunA17_Chr6g0449641 42.623 61 35 0 9 69 10 70 8.12e-12 57.0
MsaG044701 MtrunA17_Chr2g0311601 44.262 61 34 0 9 69 14 74 1.42e-11 56.6
MsaG044701 MtrunA17_Chr4g0043421 44.262 61 34 0 9 69 16 76 1.85e-11 56.6
MsaG044701 MtrunA17_Chr4g0040831 34.328 67 44 0 3 69 10 76 4.16e-11 56.2
MsaG044701 MtrunA17_Chr5g0438091 44.262 61 34 0 9 69 40 100 5.64e-11 55.8
MsaG044701 MtrunA17_Chr3g0129071 45.312 64 35 0 6 69 45 108 6.75e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044701 AT3G26660.1 46.774 62 33 0 7 68 4 65 1.76e-15 66.2
MsaG044701 AT3G26620.1 46.774 62 33 0 7 68 4 65 2.29e-15 65.9
MsaG044701 AT1G65620.1 49.180 61 31 0 9 69 10 70 1.34e-14 65.9
MsaG044701 AT1G65620.4 49.180 61 31 0 9 69 10 70 1.34e-14 65.9
MsaG044701 AT1G65620.3 49.180 61 31 0 9 69 10 70 1.34e-14 65.9
MsaG044701 AT1G65620.5 49.180 61 31 0 9 69 10 70 1.34e-14 65.9
MsaG044701 AT1G65620.2 49.180 61 31 0 9 69 10 70 1.34e-14 65.9
MsaG044701 AT2G30130.1 49.180 61 31 0 9 69 9 69 7.49e-14 63.5
MsaG044701 AT2G23660.2 50.000 60 30 0 9 68 6 65 9.34e-14 64.7
MsaG044701 AT2G23660.3 50.000 60 30 0 9 68 6 65 9.34e-14 64.7
MsaG044701 AT2G23660.1 50.000 60 30 0 9 68 6 65 9.34e-14 64.7
MsaG044701 AT5G66870.1 47.541 61 32 0 9 69 8 68 1.86e-13 63.9
MsaG044701 AT3G27650.1 45.902 61 33 0 9 69 40 100 2.56e-13 61.6
MsaG044701 AT1G31320.1 44.262 61 34 0 9 69 14 74 1.59e-12 59.7
MsaG044701 AT1G72980.1 39.344 61 37 0 9 69 14 74 3.13e-12 59.7
MsaG044701 AT2G28500.1 41.538 65 38 0 5 69 52 116 3.31e-12 59.7
MsaG044701 AT1G07900.1 40.580 69 41 0 5 73 30 98 3.93e-12 58.9
MsaG044701 AT5G63090.4 44.262 61 34 0 9 69 12 72 4.21e-12 58.9
MsaG044701 AT5G63090.3 44.262 61 34 0 9 69 12 72 4.21e-12 58.9
MsaG044701 AT5G63090.2 44.262 61 34 0 9 69 12 72 4.21e-12 58.9
MsaG044701 AT5G63090.1 44.262 61 34 0 9 69 12 72 4.21e-12 58.9
MsaG044701 AT2G40470.2 45.312 64 35 0 6 69 18 81 2.38e-11 57.0
MsaG044701 AT2G40470.1 45.312 64 35 0 6 69 43 106 3.42e-11 57.0
MsaG044701 AT1G16530.1 43.548 62 34 1 9 69 15 76 3.74e-11 56.2
MsaG044701 AT3G11090.1 40.000 65 39 0 9 73 12 76 8.26e-11 55.1

Find 16 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAGGTACACTCTGTGTT+TGG 0.360225 8:+63674842 MsaT044701.1:CDS
AGGTTGTTGGGTGGAATGTT+AGG 0.398338 8:-63674809 None:intergenic
ACATGTTATTCTTCAGCAAC+AGG 0.412870 8:-63676629 None:intergenic
GACGGAGATGCTTGCACGCT+GGG 0.417388 8:-63674757 None:intergenic
ATGAAATGTTGCAGGTTGTT+GGG 0.430961 8:-63674821 None:intergenic
TGAACAAGCTATTGAATCCT+TGG 0.435375 8:+63674913 MsaT044701.1:CDS
GATGAAATGTTGCAGGTTGT+TGG 0.445023 8:-63674822 None:intergenic
GTACCTTGATGAAATGTTGC+AGG 0.448404 8:-63674829 None:intergenic
CGACGGAGATGCTTGCACGC+TGG 0.511634 8:-63674758 None:intergenic
CAGCGTGCAAGCATCTCCGT+CGG 0.548053 8:+63674759 MsaT044701.1:CDS
AGCGTGCAAGCATCTCCGTC+GGG 0.559116 8:+63674760 MsaT044701.1:CDS
AAATGTTGCAGGTTGTTGGG+TGG 0.580347 8:-63674818 None:intergenic
TATTATTAGTGGTAGCAATG+AGG 0.599767 8:+63675896 MsaT044701.1:intron
TGGGTGGAATGTTAGGAGCA+AGG 0.620513 8:-63674802 None:intergenic
CAACCTGCAACATTTCATCA+AGG 0.635459 8:+63674826 MsaT044701.1:CDS
AACTTGAGTCACACTCCCGA+CGG 0.656492 8:-63674775 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 63674731 63676646 63674731 ID=MsaG044701
Chr8 mRNA 63674731 63676646 63674731 ID=MsaT044701.1;Parent=MsaG044701
Chr8 exon 63674731 63674936 63674731 ID=MsaT044701.1.exon1;Parent=MsaT044701.1
Chr8 CDS 63674731 63674936 63674731 ID=cds.MsaT044701.1;Parent=MsaT044701.1
Chr8 exon 63675905 63675917 63675905 ID=MsaT044701.1.exon2;Parent=MsaT044701.1
Chr8 CDS 63675905 63675917 63675905 ID=cds.MsaT044701.1;Parent=MsaT044701.1
Chr8 exon 63676605 63676646 63676605 ID=MsaT044701.1.exon3;Parent=MsaT044701.1
Chr8 CDS 63676605 63676646 63676605 ID=cds.MsaT044701.1;Parent=MsaT044701.1
Gene Sequence

>MsaT044701.1

ATGGCAAGTGCGATAATTCGTCTGTGCCCAGCGTGCAAGCATCTCCGTCGGGAGTGTGACTCAAGTTGTGACCTTGCTCCTAACATTCCACCCAACAACCTGCAACATTTCATCAAGGTACACTCTGTGTTTGGAAAGAACAATGTGTCGAAGATGTTGAAAAAACTTGATGTTTCACATCATGAACAAGCTATTGAATCCTTGGTTGGTAGCAATGAGAAGATGTATCAGCAAACATTGTTGCCTGTTGCTGAAGAATAA
Protein sequence

>MsaT044701.1

MASAIIRLCPACKHLRRECDSSCDLAPNIPPNNLQHFIKVHSVFGKNNVSKMLKKLDVSHHEQAIESLVGSNEKMYQQTLLPVAEE*