AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044729

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044729 MtrunA17_Chr4g0055441 90.385 156 15 0 1 156 1 156 1.05e-105 298
MsaG044729 MtrunA17_Chr5g0439111 64.925 134 42 1 2 130 9 142 5.77e-52 165
MsaG044729 MtrunA17_Chr6g0452661 48.387 186 54 3 2 146 9 193 4.22e-50 160
MsaG044729 MtrunA17_Chr6g0452691 51.656 151 72 1 2 151 9 159 4.83e-50 158
MsaG044729 MtrunA17_Chr6g0452671 51.974 152 61 4 2 143 11 160 9.24e-46 148
MsaG044729 MtrunA17_Chr6g0449311 67.059 85 25 1 30 111 1 85 5.83e-34 116
MsaG044729 MtrunA17_Chr8g0342351 52.427 103 48 1 2 104 12 113 2.47e-30 110
MsaG044729 MtrunA17_Chr4g0059901 48.113 106 54 1 2 107 10 114 9.31e-29 106
MsaG044729 MtrunA17_Chr2g0330201 47.170 106 55 1 2 107 19 123 1.03e-27 104
MsaG044729 MtrunA17_Chr8g0353981 52.500 80 37 1 3 82 8 86 8.77e-27 100
MsaG044729 MtrunA17_Chr7g0225111 38.889 108 65 1 3 110 6 112 1.84e-26 96.7
MsaG044729 MtrunA17_Chr4g0058651 37.008 127 79 1 3 129 12 137 8.77e-26 97.1
MsaG044729 MtrunA17_Chr5g0402611 38.636 132 76 3 3 130 9 139 1.10e-25 99.4
MsaG044729 MtrunA17_Chr2g0311601 36.937 111 69 1 3 113 14 123 3.51e-25 94.4
MsaG044729 MtrunA17_Chr4g0043421 32.143 140 93 2 3 141 16 154 6.84e-25 94.4
MsaG044729 MtrunA17_Chr3g0115431 43.137 102 57 1 3 104 10 110 9.07e-25 95.1
MsaG044729 MtrunA17_Chr3g0117121 43.158 95 53 1 3 97 11 104 1.07e-24 94.4
MsaG044729 MtrunA17_Chr7g0227511 42.105 95 54 1 3 97 12 105 1.10e-24 93.6
MsaG044729 MtrunA17_Chr3g0097221 42.157 102 58 1 3 104 8 108 2.23e-23 92.8
MsaG044729 MtrunA17_Chr6g0449641 42.105 95 54 1 3 97 10 103 5.25e-23 88.6
MsaG044729 MtrunA17_Chr5g0444351 35.294 102 65 1 3 104 6 106 3.07e-22 86.7
MsaG044729 MtrunA17_Chr8g0374591 41.176 102 59 1 3 104 9 109 3.18e-22 86.3
MsaG044729 MtrunA17_Chr4g0040831 41.000 100 58 1 2 101 15 113 3.53e-22 87.8
MsaG044729 MtrunA17_Chr8g0374601 43.137 102 57 1 3 104 27 127 3.57e-22 89.7
MsaG044729 MtrunA17_Chr3g0106101 35.897 117 73 2 3 119 8 122 1.18e-21 85.9
MsaG044729 MtrunA17_Chr5g0437571 39.326 89 53 1 3 91 7 94 1.33e-21 85.5
MsaG044729 MtrunA17_Chr6g0449651 40.000 95 56 1 3 97 68 161 1.44e-21 86.3
MsaG044729 MtrunA17_Chr4g0030001 44.211 95 47 3 3 96 25 114 1.54e-21 85.9
MsaG044729 MtrunA17_Chr5g0438091 36.735 98 61 1 3 100 40 136 1.69e-21 85.9
MsaG044729 MtrunA17_Chr5g0437401 33.846 130 77 3 2 127 11 135 1.69e-21 85.5
MsaG044729 MtrunA17_Chr1g0200201 38.053 113 67 2 3 113 73 184 9.13e-21 84.7
MsaG044729 MtrunA17_Chr3g0113891 39.175 97 54 2 2 98 14 105 4.71e-20 82.0
MsaG044729 MtrunA17_Chr5g0432451 34.426 122 79 1 3 124 44 164 5.02e-20 82.8
MsaG044729 MtrunA17_Chr3g0129071 37.500 104 63 2 3 105 48 150 1.38e-19 82.0
MsaG044729 MtrunA17_Chr3g0114061 37.234 94 58 1 3 96 9 101 1.65e-19 79.7
MsaG044729 MtrunA17_Chr8g0351721 36.522 115 71 2 3 116 19 132 4.16e-18 77.4
MsaG044729 MtrunA17_Chr8g0351701 37.864 103 62 2 3 104 24 125 4.77e-18 77.8
MsaG044729 MtrunA17_Chr7g0260971 37.500 104 63 2 3 105 15 117 7.29e-18 77.0
MsaG044729 MtrunA17_Chr8g0374581 34.400 125 79 3 3 125 9 132 3.91e-16 72.4
MsaG044729 MtrunA17_Chr6g0460951 35.294 102 64 2 3 103 41 141 1.62e-15 70.9
MsaG044729 MtrunA17_Chr6g0460941 38.202 89 53 2 3 90 8 95 2.46e-15 67.4
MsaG044729 MtrunA17_Chr4g0046201 35.556 90 57 1 2 91 6 94 2.60e-15 70.9
MsaG044729 MtrunA17_Chr7g0245871 36.000 100 62 2 3 101 28 126 8.97e-15 69.3
MsaG044729 MtrunA17_Chr2g0326041 54.167 48 22 0 3 50 34 81 1.10e-14 65.5
MsaG044729 MtrunA17_Chr5g0435641 30.769 104 70 2 2 104 7 109 1.31e-14 67.8
MsaG044729 MtrunA17_Chr1g0184281 35.354 99 62 2 3 100 16 113 1.85e-14 67.8
MsaG044729 MtrunA17_Chr8g0337271 29.452 146 96 4 1 140 9 153 5.59e-14 66.2
MsaG044729 MtrunA17_Chr1g0184271 31.313 99 66 2 3 100 13 110 3.05e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044729 AT3G47870.1 52.941 102 47 1 2 103 36 136 1.41e-30 112
MsaG044729 AT3G13850.1 48.544 103 52 1 2 104 36 137 9.55e-28 104
MsaG044729 AT3G26660.1 41.176 102 59 1 3 104 6 106 7.70e-26 95.5
MsaG044729 AT3G26620.1 41.176 102 59 1 3 104 6 106 1.16e-25 95.1
MsaG044729 AT5G63090.4 41.748 103 59 1 3 105 12 113 1.92e-24 93.6
MsaG044729 AT5G63090.3 41.748 103 59 1 3 105 12 113 1.92e-24 93.6
MsaG044729 AT5G63090.2 41.748 103 59 1 3 105 12 113 1.92e-24 93.6
MsaG044729 AT5G63090.1 41.748 103 59 1 3 105 12 113 1.92e-24 93.6
MsaG044729 AT1G65620.1 47.500 80 41 1 3 82 10 88 9.58e-24 92.4
MsaG044729 AT1G65620.4 47.500 80 41 1 3 82 10 88 9.58e-24 92.4
MsaG044729 AT1G65620.3 47.500 80 41 1 3 82 10 88 9.58e-24 92.4
MsaG044729 AT1G65620.5 47.500 80 41 1 3 82 10 88 9.58e-24 92.4
MsaG044729 AT1G65620.2 47.500 80 41 1 3 82 10 88 9.58e-24 92.4
MsaG044729 AT5G66870.1 40.196 102 60 1 3 104 8 108 1.29e-23 94.4
MsaG044729 AT3G27650.1 39.423 104 61 2 3 105 40 142 1.29e-22 88.2
MsaG044729 AT1G31320.1 42.529 87 49 1 3 89 14 99 1.36e-22 88.6
MsaG044729 AT1G72980.1 36.000 150 89 3 2 151 13 155 1.56e-22 89.4
MsaG044729 AT1G07900.1 43.617 94 48 2 3 96 34 122 2.66e-22 88.2
MsaG044729 AT2G23660.2 42.553 94 53 1 3 96 6 98 1.55e-21 88.6
MsaG044729 AT2G23660.3 42.553 94 53 1 3 96 6 98 1.55e-21 88.6
MsaG044729 AT2G23660.1 42.553 94 53 1 3 96 6 98 1.55e-21 88.6
MsaG044729 AT1G16530.1 40.741 108 55 3 3 102 15 121 3.58e-21 84.7
MsaG044729 AT2G28500.1 42.553 94 49 2 3 96 56 144 4.89e-21 85.9
MsaG044729 AT3G11090.1 39.175 97 54 2 2 98 11 102 6.53e-21 84.0
MsaG044729 AT2G30130.1 36.441 118 71 3 3 120 9 122 1.00e-20 84.3
MsaG044729 AT3G50510.1 34.746 118 75 2 3 119 13 129 1.05e-20 84.3
MsaG044729 AT3G50510.2 34.746 118 75 2 3 119 13 129 1.05e-20 84.3
MsaG044729 AT5G35900.1 36.275 102 64 1 3 104 6 106 1.47e-20 84.0
MsaG044729 AT2G40470.2 37.383 107 66 1 3 109 21 126 3.36e-20 83.2
MsaG044729 AT2G40470.1 37.383 107 66 1 3 109 46 151 6.41e-20 82.8
MsaG044729 AT2G30340.2 34.454 119 77 1 3 121 54 171 6.48e-20 83.6
MsaG044729 AT2G30340.1 34.454 119 77 1 3 121 53 170 6.80e-20 83.6
MsaG044729 AT1G06280.1 39.326 89 53 1 2 90 24 111 5.98e-18 77.4
MsaG044729 AT5G15060.1 41.667 84 47 2 3 85 18 100 3.45e-17 73.9
MsaG044729 AT2G19820.1 36.264 91 56 1 3 91 13 103 2.26e-15 68.6
MsaG044729 AT4G00220.1 36.634 101 62 2 5 104 20 119 2.64e-15 70.9
MsaG044729 AT2G45420.1 35.644 101 63 2 5 104 40 139 3.58e-15 70.9
MsaG044729 AT2G42430.1 40.741 81 46 2 3 82 16 95 1.85e-14 68.6
MsaG044729 AT5G06080.1 30.263 152 92 5 2 148 7 149 2.73e-14 67.0
MsaG044729 AT4G00210.1 32.031 128 78 3 3 122 12 138 5.95e-14 67.0
MsaG044729 AT3G58190.1 31.429 140 78 4 3 139 12 136 9.01e-14 66.6
MsaG044729 AT3G03760.1 34.314 102 65 2 3 103 52 152 1.58e-13 66.6
MsaG044729 AT2G45410.1 34.545 110 70 2 5 113 19 127 1.76e-13 65.1
MsaG044729 AT2G31310.1 34.091 88 56 2 3 89 8 94 3.34e-12 61.6
MsaG044729 AT2G42440.1 34.694 98 59 3 3 96 8 104 1.32e-11 60.8
MsaG044729 AT4G22700.1 28.704 108 76 1 3 110 6 112 9.91e-11 57.8

Find 23 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGTACCAATTGGAGTATT+TGG 0.132307 8:-64091492 None:intergenic
TGAGCCTTGGCGAAGATTTC+TGG 0.170855 8:-64091210 None:intergenic
GGCGAAGATTTCTGGTTTAT+TGG 0.184703 8:-64091202 None:intergenic
GGTTTATTGGCTGGAAAATA+AGG 0.277668 8:-64091189 None:intergenic
TAAGGAGCTAAAGCGCAATT+TGG 0.321462 8:-64091171 None:intergenic
GCCAAGGCTCATCGTTTGTT+TGG 0.369225 8:+64091222 MsaT044729.1:CDS
GTATTCAAATTTGTACCAAT+TGG 0.401383 8:-64091501 None:intergenic
AAGATTTCTGGTTTATTGGC+TGG 0.417871 8:-64091198 None:intergenic
CGTGCAAGATTCCCTGCTAC+TGG 0.446694 8:+64091336 MsaT044729.1:CDS
GGAAAATGAGAACTAGTAGA+AGG 0.455012 8:-64091471 None:intergenic
TTCCCTGCTACTGGATGTCT+TGG 0.464849 8:+64091345 MsaT044729.1:CDS
ACAAAGGAAGCACATTTGAA+TGG 0.476409 8:+64091540 MsaT044729.1:CDS
CTTTAGCATCTTCACCATGT+TGG 0.486560 8:-64091250 None:intergenic
ACACCAAGACATCCAGTAGC+AGG 0.506521 8:-64091348 None:intergenic
CAATGGTGGTGTTGATCCAC+AGG 0.514297 8:+64091566 MsaT044729.1:CDS
CTTGGTGTTATTCATACATA+CGG 0.518659 8:+64091363 MsaT044729.1:CDS
ACCAAACAAACGATGAGCCT+TGG 0.526005 8:-64091223 None:intergenic
TTTGTTTGGTGTTTCCAACA+TGG 0.590911 8:+64091236 MsaT044729.1:CDS
CACCAAGACATCCAGTAGCA+GGG 0.629659 8:-64091347 None:intergenic
TAAACCAGAAATCTTCGCCA+AGG 0.633091 8:+64091206 MsaT044729.1:CDS
GCACATTTGAATGGTGACAA+TGG 0.675011 8:+64091549 MsaT044729.1:CDS
CATTTGAATGGTGACAATGG+TGG 0.751872 8:+64091552 MsaT044729.1:CDS
AATGGTGGTGTTGATCCACA+GGG 0.779286 8:+64091567 MsaT044729.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 64091129 64091599 64091129 ID=MsaG044729
Chr8 mRNA 64091129 64091599 64091129 ID=MsaT044729.1;Parent=MsaG044729
Chr8 exon 64091129 64091599 64091129 ID=MsaT044729.1.exon1;Parent=MsaT044729.1
Chr8 CDS 64091129 64091599 64091129 ID=cds.MsaT044729.1;Parent=MsaT044729.1
Gene Sequence

>MsaT044729.1

ATGGCTTGTGCAGCATGCAAATATCAAAGAAGAAAGTGTTCTCCAAATTGCGCTTTAGCTCCTTATTTTCCAGCCAATAAACCAGAAATCTTCGCCAAGGCTCATCGTTTGTTTGGTGTTTCCAACATGGTGAAGATGCTAAAGAATATTGATGATGATCATCTAAAAGATGAAGCAATGAAATCAATTATTTTTGAGTCGGAAATGCGTGCAAGATTCCCTGCTACTGGATGTCTTGGTGTTATTCATACATACGGAGATGAGTTAAAGAAGTCTCTTGAAGAGTTAGATCATGTAAGAAATCTACTAGCTTATTGTAAACAACATAATCTTTCTTCTGCACCTTCTACTAGTTCTCATTTTCCAAATACTCCAATTGGTACAAATTTGAATACAAGTTTTATGGATTCAACAAAGGAAGCACATTTGAATGGTGACAATGGTGGTGTTGATCCACAGGGAAAACCTTAA
Protein sequence

>MsaT044729.1

MACAACKYQRRKCSPNCALAPYFPANKPEIFAKAHRLFGVSNMVKMLKNIDDDHLKDEAMKSIIFESEMRARFPATGCLGVIHTYGDELKKSLEELDHVRNLLAYCKQHNLSSAPSTSSHFPNTPIGTNLNTSFMDSTKEAHLNGDNGGVDPQGKP*