AlfalfaGEDB | MsaG044734

M. sativa ssp. caerulea / MsaG044734

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044734	XP_024634520.2	93.443	122	8	0	1	122	1	122	1.36e-66	235
MsaG044734	XP_024634520.2	93.818	275	17	0	117	391	480	754	4.94e-175	516

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044734	sp\|Q9ZVC9\|FRS3_ARATH	33.533	167	108	2	117	283	466	629	7.25e-24	107

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044734	tr\|A0A2Z6P1T9\|A0A2Z6P1T9_TRISU	71.273	275	77	2	117	390	462	735	1.32e-132	407
MsaG044734	tr\|A0A2Z6P1T9\|A0A2Z6P1T9_TRISU	77.320	97	22	0	26	122	8	104	7.95e-42	167

TFs/TRs:

Gene ID	Type	Classification
MsaG044734	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000537	MsaG044734	0.812422	7.348886e-51	1.331728e-48
MsaG001145	MsaG044734	0.809993	2.456738e-50	4.194761e-48
MsaG003310	MsaG044734	0.803877	4.762199e-49	7.034182e-47
MsaG004400	MsaG044734	0.829783	7.621426e-55	2.192039e-52
MsaG005460	MsaG044734	0.804444	3.634298e-49	5.439153e-47
MsaG006463	MsaG044734	0.844956	1.027490e-58	4.702038e-56
MsaG006566	MsaG044734	0.800155	2.749875e-48	3.730991e-46
MsaG007434	MsaG044734	0.807243	9.443136e-50	1.509066e-47
MsaG007447	MsaG044734	0.801407	1.531427e-48	2.137438e-46
MsaG008106	MsaG044734	0.843424	2.636328e-58	1.147552e-55
MsaG011559	MsaG044734	0.817750	4.883178e-52	1.012666e-49
MsaG042502	MsaG044734	0.837361	9.976459e-57	3.587896e-54
MsaG044734	MsaG044915	0.806211	1.555710e-49	2.426495e-47
MsaG044734	MsaG045667	0.818525	3.267629e-52	6.913607e-50
MsaG044734	MsaG001347	0.852564	8.152519e-61	4.829968e-58
MsaG044734	MsaG002643	0.830496	5.113599e-55	1.500959e-52
MsaG044734	MsaG008262	0.815392	1.638628e-51	3.199241e-49
MsaG044734	MsaG014987	0.818237	3.794799e-52	7.969303e-50
MsaG044734	MsaG012407	0.804071	4.341676e-49	6.442321e-47
MsaG044734	MsaG026151	0.810647	1.778470e-50	3.085421e-48
MsaG044734	MsaG031429	0.817742	4.903736e-52	1.016714e-49
MsaG044734	MsaG036864	0.841143	1.053064e-57	4.261545e-55
MsaG017407	MsaG044734	0.801095	1.772054e-48	2.455863e-46
MsaG017884	MsaG044734	0.825866	6.596406e-54	1.699585e-51
MsaG018442	MsaG044734	0.807415	8.686346e-50	1.393865e-47
MsaG018493	MsaG044734	0.801609	1.392550e-48	1.952553e-46
MsaG019301	MsaG044734	0.847020	2.839672e-59	1.391487e-56
MsaG019325	MsaG044734	0.842246	5.404097e-58	2.264940e-55
MsaG019355	MsaG044734	0.818881	2.715433e-52	5.798770e-50
MsaG021666	MsaG044734	0.800653	2.179626e-48	2.990876e-46
MsaG027688	MsaG044734	0.804172	4.138477e-49	6.154883e-47
MsaG027689	MsaG044734	0.805275	2.441172e-49	3.724640e-47
MsaG028645	MsaG044734	0.800376	2.480549e-48	3.382461e-46
MsaG028984	MsaG044734	0.804468	3.594022e-49	5.381813e-47
MsaG032638	MsaG044734	0.817042	7.039587e-52	1.433549e-49
MsaG033977	MsaG044734	0.818444	3.408329e-52	7.196046e-50
MsaG039607	MsaG044734	0.816943	7.405190e-52	1.504152e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044734	MtrunA17_Chr5g0448761	29.412	204	138	2	117	320	512	709	7.06e-25	107
MsaG044734	MtrunA17_Chr5g0448511	29.787	188	132	0	117	304	284	471	1.04e-23	102
MsaG044734	MtrunA17_Chr5g0440451	37.931	116	72	0	117	232	491	606	1.25e-22	100
MsaG044734	MtrunA17_Chr3g0118611	35.338	133	86	0	115	247	386	518	6.38e-21	95.1
MsaG044734	MtrunA17_Chr1g0202341	36.111	108	69	0	124	231	503	610	5.09e-17	83.2
MsaG044734	MtrunA17_Chr3g0080351	28.139	231	146	5	94	321	490	703	2.02e-14	75.1
MsaG044734	MtrunA17_Chr1g0207551	32.787	122	81	1	118	239	478	598	1.15e-13	72.8
MsaG044734	MtrunA17_Chr2g0287061	29.630	162	108	4	124	283	594	751	3.25e-13	71.2
MsaG044734	MtrunA17_Chr2g0289081	54.902	51	23	0	37	87	64	114	3.34e-12	63.5
MsaG044734	MtrunA17_Chr2g0329031	33.929	112	72	2	118	229	451	560	9.81e-12	66.6
MsaG044734	MtrunA17_Chr2g0291581	30.328	122	78	3	117	231	501	622	2.97e-11	65.1
MsaG044734	MtrunA17_Chr8g0363841	27.861	201	126	6	127	315	473	666	3.91e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044734	AT2G27110.2	33.533	167	108	2	117	283	466	629	7.37e-25	107
MsaG044734	AT2G27110.1	33.533	167	108	2	117	283	466	629	7.37e-25	107
MsaG044734	AT2G27110.3	34.706	170	102	4	117	283	321	484	9.67e-25	106
MsaG044734	AT4G38180.1	37.607	117	73	0	117	233	521	637	2.07e-22	99.8
MsaG044734	AT4G38170.1	37.069	116	73	0	114	229	283	398	6.79e-19	89.0
MsaG044734	AT4G19990.6	35.246	122	77	2	118	238	446	566	2.38e-15	78.6
MsaG044734	AT4G19990.2	35.246	122	77	2	118	238	446	566	2.38e-15	78.6
MsaG044734	AT4G19990.3	35.246	122	77	2	118	238	446	566	2.83e-15	78.2
MsaG044734	AT4G19990.4	35.246	122	77	2	118	238	446	566	2.83e-15	78.2
MsaG044734	AT4G19990.7	35.246	122	77	2	118	238	460	580	2.96e-15	78.2
MsaG044734	AT4G19990.5	35.246	122	77	2	118	238	446	566	3.01e-15	78.2
MsaG044734	AT4G19990.1	35.246	122	77	2	118	238	389	509	3.40e-15	77.8
MsaG044734	AT3G06250.2	30.625	160	108	2	124	283	598	754	5.03e-15	77.4
MsaG044734	AT3G06250.1	30.625	160	108	2	124	283	598	754	5.03e-15	77.4
MsaG044734	AT3G06250.3	30.625	160	108	2	124	283	598	754	5.03e-15	77.4
MsaG044734	AT2G32250.2	26.590	173	121	2	124	296	448	614	3.45e-14	74.7
MsaG044734	AT2G32250.4	26.590	173	121	2	124	296	448	614	3.45e-14	74.7
MsaG044734	AT2G32250.6	26.590	173	121	2	124	296	448	614	3.62e-14	74.7
MsaG044734	AT2G32250.1	26.590	173	121	2	124	296	448	614	3.62e-14	74.7
MsaG044734	AT2G32250.3	26.590	173	121	2	124	296	448	614	3.62e-14	74.7
MsaG044734	AT2G32250.5	26.590	173	121	2	124	296	448	614	4.17e-14	74.7
MsaG044734	AT2G32250.8	26.590	173	121	2	124	296	407	573	4.87e-14	74.3
MsaG044734	AT2G32250.7	26.590	173	121	2	124	296	407	573	5.38e-14	74.3
MsaG044734	AT1G52520.1	33.071	127	76	3	113	231	514	639	1.53e-13	72.8
MsaG044734	AT5G18960.2	29.375	160	110	2	124	283	622	778	1.68e-13	72.8
MsaG044734	AT5G18960.1	29.375	160	110	2	124	283	622	778	1.68e-13	72.8
MsaG044734	AT4G15090.1	32.787	122	81	1	112	233	492	612	8.40e-13	70.5
MsaG044734	AT4G15090.5	32.787	122	81	1	112	233	492	612	8.40e-13	70.5
MsaG044734	AT4G15090.3	32.787	122	81	1	112	233	492	612	8.40e-13	70.5
MsaG044734	AT4G15090.2	32.787	122	81	1	112	233	492	612	8.40e-13	70.5
MsaG044734	AT4G15090.4	32.787	122	81	1	112	233	492	612	8.40e-13	70.5
MsaG044734	AT3G22170.2	32.143	112	75	1	118	229	507	617	1.97e-12	69.3
MsaG044734	AT3G22170.1	32.143	112	75	1	118	229	507	617	1.97e-12	69.3
MsaG044734	AT1G76320.2	32.143	112	73	1	118	229	435	543	7.16e-12	67.4
MsaG044734	AT1G76320.1	32.143	112	73	1	118	229	435	543	7.36e-12	67.4
MsaG044734	AT1G76320.4	32.143	112	73	1	118	229	435	543	7.36e-12	67.4
MsaG044734	AT1G76320.3	32.143	112	73	1	118	229	435	543	8.49e-12	67.4

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTCTCGTTGATGTAAATT+TGG	0.181383	8:+64155546	MsaT044734.1:CDS
CTTCCTCCGACAAGCAATTT+TGG	0.188112	8:-64155960	None:intergenic
TAGTTTGATTTGTGTCTTCT+TGG	0.191519	8:-64154214	None:intergenic
TTTACAATTCATTTGCAAAA+AGG	0.228560	8:+64154087	MsaT044734.1:CDS
TGCATCCTTCTTAATATCTT+TGG	0.239826	8:-64155766	None:intergenic
AGCAAAGGTATTAGTTGATT+TGG	0.254141	8:+64155801	MsaT044734.1:CDS
GGAGTTTCTCCTTTGGAATC+TGG	0.287253	8:+64155925	MsaT044734.1:CDS
GGATTTCTAATAGTCAATTC+TGG	0.312090	8:-64155991	None:intergenic
GGTAAATTTCAAGATGAGAT+TGG	0.337382	8:+64155433	MsaT044734.1:CDS
ATTAAATAGTGAACCTTGTA+AGG	0.338372	8:+64154028	MsaT044734.1:CDS
CATACAAAGACAGCTCTCTT+TGG	0.340587	8:-64154143	None:intergenic
ATCTCGAGCATTGTAGTTGC+TGG	0.348302	8:-64155521	None:intergenic
TTTAATTCGTCACCAACATT+AGG	0.350028	8:-64154011	None:intergenic
CAGTGATGGAGTTTCTCCTT+TGG	0.356600	8:+64155918	MsaT044734.1:CDS
TCCAACGCTGCAAAATAAAA+TGG	0.360936	8:-64155675	None:intergenic
GAAACTTGATAGACATAGTT+AGG	0.397106	8:-64155499	None:intergenic
GATATTGTGCAGGCATATAC+TGG	0.399955	8:+64155615	MsaT044734.1:CDS
GTAAATTTCAAGATGAGATT+GGG	0.418089	8:+64155434	MsaT044734.1:CDS
AAGTGTTCTACTTCTCGAAC+TGG	0.426697	8:+64154242	MsaT044734.1:CDS
CCACGCAATGCACAAACAAA+AGG	0.435072	8:+64156012	MsaT044734.1:CDS
AATTCATTATATCTGATTTG+AGG	0.445916	8:+64155848	MsaT044734.1:CDS
CGCTGCAAAATAAAATGGTC+AGG	0.446582	8:-64155670	None:intergenic
GGAATTCAAATCTGTGGAAA+AGG	0.451124	8:+64154055	MsaT044734.1:CDS
TGTCATGAGTGTGGTGAATC+TGG	0.451337	8:+64156132	MsaT044734.1:CDS
TTGAATTCCATCCCCTTACA+AGG	0.484462	8:-64154041	None:intergenic
TTAGCTGCTGCTCCTAATGT+TGG	0.490364	8:+64153999	MsaT044734.1:CDS
ATTTGATTTCTTCATTGCAA+TGG	0.501662	8:-64155887	None:intergenic
GCCACACTTGTTGTCAAAAG+AGG	0.505706	8:+64154272	MsaT044734.1:CDS
CAAAATTGTAGATTCAAAAG+TGG	0.511466	8:+64156063	MsaT044734.1:CDS
GTCTTTGTATGTTGTAATGA+AGG	0.517879	8:+64154155	MsaT044734.1:CDS
TGATTTGAGGCGCACTCGTA+TGG	0.520232	8:+64155861	MsaT044734.1:CDS
TAGTGAACCTTGTAAGGGGA+TGG	0.531310	8:+64154034	MsaT044734.1:CDS
CACGCAATGCACAAACAAAA+GGG	0.538817	8:+64156013	MsaT044734.1:CDS
TGTCAACCAAAATTGCTTGT+CGG	0.544243	8:+64155954	MsaT044734.1:CDS
TCAAATTTAGCTATCAGTGA+TGG	0.553213	8:+64155904	MsaT044734.1:CDS
TTAAATAGTGAACCTTGTAA+GGG	0.561628	8:+64154029	MsaT044734.1:CDS
GCACGCCAAAGATATTAAGA+AGG	0.572798	8:+64155761	MsaT044734.1:CDS
GTCTACAGGGTCAAAACTTG+AGG	0.581238	8:+64155375	MsaT044734.1:intron
GGGGATGGAATTCAAATCTG+TGG	0.582477	8:+64154049	MsaT044734.1:CDS
ACAAACAAAAGGGAGAAAGA+AGG	0.601052	8:+64156023	MsaT044734.1:CDS
GCATGCTCAACAAGAAGCAA+AGG	0.605686	8:+64155786	MsaT044734.1:CDS
TTCCACATTTGTTGACTGTG+TGG	0.606600	8:-64153958	None:intergenic
GTGCATTGGTCAATTATGGA+TGG	0.620199	8:+64153918	None:intergenic
AGCCACACAGTCAACAAATG+TGG	0.622019	8:+64153956	MsaT044734.1:CDS
ACAAAAGGGAGAAAGAAGGA+TGG	0.625355	8:+64156027	MsaT044734.1:CDS
TAAATAGTGAACCTTGTAAG+GGG	0.625905	8:+64154030	MsaT044734.1:CDS
GACACACAGATTGCAAAATG+TGG	0.629854	8:+64155568	MsaT044734.1:CDS
CAACCAAAATTGCTTGTCGG+AGG	0.633822	8:+64155957	MsaT044734.1:CDS
AGAAAAGCATGTCATGAGTG+TGG	0.709074	8:+64156123	MsaT044734.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	64153933	64156197	64153933	ID=MsaG044734
Chr8	mRNA	64153933	64156197	64153933	ID=MsaT044734.1;Parent=MsaG044734
Chr8	exon	64153933	64154293	64153933	ID=MsaT044734.1.exon1;Parent=MsaT044734.1
Chr8	CDS	64153933	64154293	64153933	ID=cds.MsaT044734.1;Parent=MsaT044734.1
Chr8	exon	64155383	64156197	64155383	ID=MsaT044734.1.exon2;Parent=MsaT044734.1
Chr8	CDS	64155383	64156197	64155383	ID=cds.MsaT044734.1;Parent=MsaT044734.1

Gene Sequence

>MsaT044734.1

ATGGATGGTAATGTTTTTGAAGAAGCCACACAGTCAACAAATGTGGAAATATCTGAAGAAGATATTTTAGCTGCTGCTCCTAATGTTGGTGACGAATTAAATAGTGAACCTTGTAAGGGGATGGAATTCAAATCTGTGGAAAAGGTTAGAGAATTTTACAATTCATTTGCAAAAAGGATGGGTTTTGGAGTACGTGTTCGCTCAACTAAACCAAAGAGAGCTGTCTTTGTATGTTGTAATGAAGGTCATCATATAGTGAAAAGTTCAATAAACAAAGAAATCCAAGAAGACACAAATCAAACTAAAAGAAAGTGTTCTACTTCTCGAACTGGTTGTCTAGCCACACTTGTTGTCAAAAGAGGGTCAAAACTTGAGGAGCACGCTGCATCTATTTATTCGAGAAACATTTTTGGTAAATTTCAAGATGAGATTGGGAAGATCTACAAATTTGCAAAAAAGAAGATTAGCAAAGATGGACCTAACTATGTCTATCAAGTTTCCAGCAACTACAATGCTCGAGATATGTTTCTCGTTGATGTAAATTTGGACACACAGATTGCAAAATGTGGATGTCAATTATATGAGTTTTTGGGGATATTGTGCAGGCATATACTGGTAATTTTTCAAGCAAAAGGTATCACTCAAATTCCTGACCATTTTATTTTGCAGCGTTGGACTAAAAATGCTAACAAAAGCATTGAAGTTTGTGATACGACGAGTAATTTTGATGGTCAAAATAGCACGCCAAAGATATTAAGAAGGATGCATGCTCAACAAGAAGCAAAGGTATTAGTTGATTTGGCTGAAGAGTCTGATGAGATATACAAATTCATTATATCTGATTTGAGGCGCACTCGTATGGCAGCCATTGCAATGAAGAAATCAAATTTAGCTATCAGTGATGGAGTTTCTCCTTTGGAATCTGGCCAAAATGTCAACCAAAATTGCTTGTCGGAGGAAGAAAGTGAACCAGAATTGACTATTAGAAATCCACGCAATGCACAAACAAAAGGGAGAAAGAAGGATGGACAGAAAAACACTCAAAATTGTAGATTCAAAAGTGGAATTGAACTGTCACATGCTCAACAAGTAGCAGTCAAAAGAAAAGCATGTCATGAGTGTGGTGAATCTGGTCACAACAGCAGAACTTGCAAGAAAAGAAATCAAAATGATTAA

Protein sequence

>MsaT044734.1

MDGNVFEEATQSTNVEISEEDILAAAPNVGDELNSEPCKGMEFKSVEKVREFYNSFAKRMGFGVRVRSTKPKRAVFVCCNEGHHIVKSSINKEIQEDTNQTKRKCSTSRTGCLATLVVKRGSKLEEHAASIYSRNIFGKFQDEIGKIYKFAKKKISKDGPNYVYQVSSNYNARDMFLVDVNLDTQIAKCGCQLYEFLGILCRHILVIFQAKGITQIPDHFILQRWTKNANKSIEVCDTTSNFDGQNSTPKILRRMHAQQEAKVLVDLAEESDEIYKFIISDLRRTRMAAIAMKKSNLAISDGVSPLESGQNVNQNCLSEEESEPELTIRNPRNAQTKGRKKDGQKNTQNCRFKSGIELSHAQQVAVKRKACHECGESGHNSRTCKKRNQND*