AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044767

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044767 MtrunA17_Chr4g0055851 93.929 280 8 3 1 271 1 280 0.0 527
MsaG044767 MtrunA17_Chr5g0399491 71.141 298 52 9 2 270 1 293 2.12e-134 381
MsaG044767 MtrunA17_Chr8g0335131 57.237 304 86 11 3 264 1 302 1.65e-88 265
MsaG044767 MtrunA17_Chr7g0258831 65.054 186 55 2 50 226 89 273 8.06e-73 225
MsaG044767 MtrunA17_Chr1g0175651 60.000 205 64 5 49 241 82 280 2.19e-69 216
MsaG044767 MtrunA17_Chr2g0308611 74.000 150 35 2 91 237 176 324 9.68e-68 213
MsaG044767 MtrunA17_Chr4g0054421 75.573 131 31 1 95 225 110 239 5.13e-63 199
MsaG044767 MtrunA17_Chr5g0400781 71.774 124 34 1 103 226 114 236 2.01e-58 187
MsaG044767 MtrunA17_Chr1g0151971 61.151 139 44 3 111 240 129 266 2.34e-52 172
MsaG044767 MtrunA17_Chr4g0044091 62.097 124 42 1 106 224 59 182 1.50e-46 154
MsaG044767 MtrunA17_Chr2g0299031 45.714 175 95 0 68 242 38 212 1.14e-42 145
MsaG044767 MtrunA17_Chr3g0135331 50.888 169 76 3 103 271 136 297 4.34e-41 143
MsaG044767 MtrunA17_Chr4g0071411 58.333 120 47 1 114 230 90 209 9.01e-39 135
MsaG044767 MtrunA17_Chr7g0217111 39.241 158 87 2 53 201 16 173 1.99e-23 96.3
MsaG044767 MtrunA17_Chr8g0362151 40.496 121 69 1 81 198 43 163 3.10e-21 90.5
MsaG044767 MtrunA17_Chr4g0060031 47.748 111 51 1 94 197 77 187 1.30e-20 89.7
MsaG044767 MtrunA17_Chr6g0453021 48.000 100 51 1 101 199 84 183 1.84e-20 88.2
MsaG044767 MtrunA17_Chr6g0450991 42.149 121 69 1 108 228 63 182 3.01e-20 87.8
MsaG044767 MtrunA17_Chr3g0119291 46.667 105 49 1 102 199 49 153 3.77e-20 87.0
MsaG044767 MtrunA17_Chr8g0381741 50.505 99 42 1 108 199 50 148 1.45e-19 86.3
MsaG044767 MtrunA17_Chr5g0416391 45.000 100 54 1 100 199 66 164 5.91e-19 84.3
MsaG044767 MtrunA17_Chr5g0415941 49.000 100 43 2 112 204 87 185 6.02e-19 84.7
MsaG044767 MtrunA17_Chr7g0265521 45.455 88 48 0 112 199 63 150 2.05e-18 81.6
MsaG044767 MtrunA17_Chr3g0123691 41.406 128 67 2 104 223 52 179 2.64e-18 82.8
MsaG044767 MtrunA17_Chr1g0179581 46.988 83 44 0 112 194 28 110 1.33e-17 76.6
MsaG044767 MtrunA17_Chr8g0362751 46.809 94 43 1 112 198 89 182 3.00e-17 79.7
MsaG044767 MtrunA17_Chr3g0127511 44.086 93 45 1 115 200 41 133 8.05e-14 69.3
MsaG044767 MtrunA17_Chr5g0410711 33.333 141 93 1 57 197 38 177 9.15e-14 71.2
MsaG044767 MtrunA17_Chr8g0346251 42.683 82 47 0 116 197 45 126 2.61e-13 67.8
MsaG044767 MtrunA17_Chr5g0403691 42.342 111 62 1 98 206 39 149 3.93e-13 67.0
MsaG044767 MtrunA17_Chr5g0403701 42.202 109 58 1 98 206 39 142 5.60e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044767 AT4G16780.1 61.034 290 83 6 2 270 1 281 2.03e-102 300
MsaG044767 AT5G47370.1 59.797 296 52 13 2 259 1 267 3.71e-96 284
MsaG044767 AT4G17460.1 57.095 296 83 10 2 270 1 279 4.08e-95 281
MsaG044767 AT3G60390.1 63.750 240 59 7 48 264 75 309 1.56e-88 266
MsaG044767 AT2G44910.1 62.780 223 48 5 27 226 67 277 9.73e-82 249
MsaG044767 AT5G06710.1 59.091 198 63 4 72 254 135 329 2.02e-68 215
MsaG044767 AT4G37790.1 76.423 123 28 1 103 225 117 238 2.75e-61 195
MsaG044767 AT2G22800.1 71.875 128 32 2 101 225 99 225 2.43e-58 187
MsaG044767 AT2G01430.1 52.326 172 65 4 73 233 95 260 5.27e-46 155
MsaG044767 AT1G70920.2 57.480 127 46 1 100 226 27 145 4.82e-37 129
MsaG044767 AT1G70920.1 57.480 127 43 2 103 226 57 175 5.28e-37 130
MsaG044767 AT5G06710.4 56.075 107 32 2 72 163 135 241 2.80e-27 107
MsaG044767 AT1G69780.1 44.444 126 65 1 79 199 47 172 1.31e-23 97.4
MsaG044767 AT2G01430.2 50.505 99 38 2 73 163 95 190 7.07e-21 88.6
MsaG044767 AT5G65310.2 34.759 187 103 5 90 269 33 207 6.41e-20 87.4
MsaG044767 AT5G65310.1 34.759 187 103 5 90 269 51 225 7.07e-20 87.8
MsaG044767 AT1G26960.1 46.875 96 51 0 104 199 63 158 8.23e-20 86.7
MsaG044767 AT3G01470.1 47.312 93 42 1 112 197 68 160 6.36e-18 81.6
MsaG044767 AT3G01220.1 42.857 119 60 4 90 201 63 180 1.94e-17 80.5
MsaG044767 AT5G15150.1 38.710 124 68 2 77 199 86 202 2.87e-17 80.5
MsaG044767 AT5G03790.1 46.067 89 48 0 112 200 77 165 2.95e-17 79.0
MsaG044767 AT4G40060.1 45.361 97 46 1 110 199 57 153 1.05e-16 78.6
MsaG044767 AT2G22430.1 46.875 96 47 1 110 201 60 155 2.09e-16 77.8
MsaG044767 AT1G27045.4 38.849 139 66 3 75 200 31 163 2.73e-16 76.6
MsaG044767 AT1G27045.1 43.119 109 50 2 75 177 31 133 3.78e-16 75.9
MsaG044767 AT1G27045.2 57.576 66 28 0 112 177 17 82 3.97e-16 74.7
MsaG044767 AT1G27045.5 57.576 66 28 0 112 177 25 90 4.06e-16 74.7
MsaG044767 AT1G27045.3 57.576 66 28 0 112 177 25 90 4.06e-16 74.7
MsaG044767 AT2G46680.2 36.508 126 71 1 115 240 35 151 7.61e-16 75.5
MsaG044767 AT2G46680.1 43.158 95 50 1 115 205 35 129 8.64e-16 75.5
MsaG044767 AT5G03790.2 43.956 91 51 0 112 202 77 167 1.37e-14 70.9
MsaG044767 AT2G36610.1 38.776 98 52 1 111 200 70 167 4.72e-14 69.3
MsaG044767 AT4G36740.1 37.624 101 63 0 98 198 41 141 1.53e-13 68.6
MsaG044767 AT4G36740.2 37.624 101 63 0 98 198 42 142 1.54e-13 68.6
MsaG044767 AT3G61890.1 42.169 83 48 0 115 197 33 115 3.72e-13 67.8
MsaG044767 AT2G18550.1 36.885 122 74 1 112 230 61 182 7.94e-13 66.6
MsaG044767 AT5G53980.1 43.678 87 49 0 112 198 11 97 2.28e-11 61.2

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGATTAATTACGGGATTA+AGG 0.204975 8:+64555827 None:intergenic
CAAACCTAGCTCTTCTGTTT+TGG 0.228150 8:+64555661 None:intergenic
AAAATCTAACGGAGGAAAAT+AGG 0.298194 8:-64555507 MsaT044767.1:CDS
CCCCTGCTTCTTCTTCCTCT+TGG 0.308178 8:+64556034 None:intergenic
TCCATCCAAACTTTCACTTC+AGG 0.343155 8:-64556354 MsaT044767.1:intron
CTGCTGCTCCAGTCACAAAT+AGG 0.366833 8:-64555279 MsaT044767.1:CDS
CATCCTCGGGCTGTGCCTAT+AGG 0.371086 8:-64555313 MsaT044767.1:CDS
TAGATTAATTACGGGATTAA+GGG 0.388703 8:+64555828 None:intergenic
ATCTAACCGTCACCATTCTA+TGG 0.389555 8:-64555344 MsaT044767.1:CDS
GCATACCTGAAGTGAAAGTT+TGG 0.401576 8:+64556349 None:intergenic
GCACAGCCCGAGGATGGGCT+TGG 0.401813 8:+64555320 None:intergenic
TTTCTTCAAGAATAGCTGAT+TGG 0.405030 8:+64555869 None:intergenic
AGCACAGTTTCAAGTGTTAG+TGG 0.413647 8:-64555997 MsaT044767.1:CDS
GCAGAAGCCCATGGGCCTAT+AGG 0.413881 8:+64555298 None:intergenic
ACACTTGAAACTGTGCTGTT+TGG 0.414647 8:+64556003 None:intergenic
CTTTATGATGATGGTTGAAA+AGG 0.420788 8:-64556439 None:intergenic
CACAGCCCGAGGATGGGCTT+GGG 0.426678 8:+64555321 None:intergenic
TACGAGGAATCGACGTGAAT+CGG 0.436608 8:-64556080 MsaT044767.1:CDS
ACATAAGAGCTTTATGATGA+TGG 0.446727 8:-64556448 None:intergenic
GCATCAACACAAGCATTAGA+TGG 0.449100 8:+64555367 None:intergenic
TGGCATTCAATAGCTGTAGA+TGG 0.450684 8:+64556054 None:intergenic
AACCAGACTTCTACTTGTCT+TGG 0.452242 8:+64555684 None:intergenic
CTATGGCCCAAGCCCATCCT+CGG 0.455411 8:-64555327 MsaT044767.1:CDS
GGCTGTGCCTATAGGCCCAT+GGG 0.462021 8:-64555305 MsaT044767.1:CDS
TAAAACGCCGTGATCATTGA+CGG 0.467131 8:+64555242 None:intergenic
GGGCTGTGCCTATAGGCCCA+TGG 0.472141 8:-64555306 MsaT044767.1:CDS
TCCTCGTACAGATCGAAACT+TGG 0.477132 8:-64556133 MsaT044767.1:intron
TCATTTGCATGTAAAATTGT+GGG 0.480628 8:+64555440 None:intergenic
TTGGCACTTGCAAAGCAACT+TGG 0.482027 8:-64555714 MsaT044767.1:CDS
GGGCACATAGTAAGAGTAGT+TGG 0.484138 8:+64555412 None:intergenic
CTATAGGCACAGCCCGAGGA+TGG 0.486518 8:+64555314 None:intergenic
GTCCAAGACAAGTAGAAGTC+TGG 0.488744 8:-64555686 MsaT044767.1:CDS
TGCCAAGAGGAAGAAGAAGC+AGG 0.500256 8:-64556036 MsaT044767.1:CDS
GATCATGACGTGGAAAATAG+AGG 0.505863 8:-64555952 MsaT044767.1:CDS
GTGAAAGTTTGGATGGAAGA+TGG 0.511827 8:+64556360 None:intergenic
ATGGTTGAAAAGGAAGACTT+GGG 0.522362 8:-64556429 MsaT044767.1:CDS
TTGATTGTGAAGTGTTGAAA+AGG 0.523538 8:-64555537 MsaT044767.1:CDS
GATGGGCTTGGGCCATAGAA+TGG 0.526314 8:+64555332 None:intergenic
ACCTGAAGTGAAAGTTTGGA+TGG 0.531944 8:+64556353 None:intergenic
TATAGGCACAGCCCGAGGAT+GGG 0.537378 8:+64555315 None:intergenic
TATGGCCCAAGCCCATCCTC+GGG 0.537951 8:-64555326 MsaT044767.1:CDS
GTCATTTGCATGTAAAATTG+TGG 0.542757 8:+64555439 None:intergenic
TGTGAAAGAAGTTCAAGCTA+AGG 0.543115 8:+64556400 None:intergenic
AAGAAGACGCTGAAACTGCT+AGG 0.546613 8:-64555915 MsaT044767.1:CDS
ACTGGAGCAGCAGAAGCCCA+TGG 0.547767 8:+64555289 None:intergenic
GATGGTTGAAAAGGAAGACT+TGG 0.551331 8:-64556430 MsaT044767.1:CDS
GCCAAGAGGAAGAAGAAGCA+GGG 0.554990 8:-64556035 MsaT044767.1:CDS
GAAACATGCAGAGGAGAGAG+TGG 0.568617 8:-64556111 MsaT044767.1:CDS
TCAAACATCCTATTTGTGAC+TGG 0.572768 8:+64555271 None:intergenic
GGTTCCAAAACAGAAGAGCT+AGG 0.575953 8:-64555665 MsaT044767.1:intron
ACAGCCACACGTTCACAAGA+AGG 0.580372 8:+64555391 None:intergenic
CTGGAGCAGCAGAAGCCCAT+GGG 0.581463 8:+64555290 None:intergenic
CACATAGTAAGAGTAGTTGG+TGG 0.588373 8:+64555415 None:intergenic
CATTTGCATGTAAAATTGTG+GGG 0.595232 8:+64555441 None:intergenic
CAGCCACACGTTCACAAGAA+GGG 0.595325 8:+64555392 None:intergenic
GGAAAATAGGAGACTACAGA+AGG 0.611602 8:-64555494 MsaT044767.1:CDS
CTTGGGCCATAGAATGGTGA+CGG 0.617650 8:+64555338 None:intergenic
CCAAGAGGAAGAAGAAGCAG+GGG 0.620587 8:-64556034 MsaT044767.1:CDS
GTGCCCTTCTTGTGAACGTG+TGG 0.623014 8:-64555395 MsaT044767.1:CDS
AAGGTGCTGTGAAAATCTAA+CGG 0.636807 8:-64555518 MsaT044767.1:CDS
TACAGCTATTGAATGCCAAG+AGG 0.669451 8:-64556049 MsaT044767.1:CDS
GGGCCTATAGGCACAGCCCG+AGG 0.674672 8:+64555310 None:intergenic
CGAAACTTGGAAACATGCAG+AGG 0.684060 8:-64556120 MsaT044767.1:CDS
GATTCACGTCGATTCCTCGT+AGG 0.688742 8:+64556082 None:intergenic
GACAAAGCTGAAGCAAACAG+AGG 0.702705 8:-64555560 MsaT044767.1:CDS
GTGCTGTGAAAATCTAACGG+AGG 0.721195 8:-64555515 MsaT044767.1:CDS
GAGAGAAGAAGATCATGACG+TGG 0.729981 8:-64555962 MsaT044767.1:CDS
GAGAGTGGAAAATACCTACG+AGG 0.764552 8:-64556096 MsaT044767.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 64555255 64556457 64555255 ID=MsaG044767
Chr8 mRNA 64555255 64556457 64555255 ID=MsaT044767.1;Parent=MsaG044767
Chr8 exon 64555255 64555582 64555255 ID=MsaT044767.1.exon4;Parent=MsaT044767.1
Chr8 CDS 64555255 64555582 64555255 ID=cds.MsaT044767.1;Parent=MsaT044767.1
Chr8 exon 64555666 64555745 64555666 ID=MsaT044767.1.exon3;Parent=MsaT044767.1
Chr8 CDS 64555666 64555745 64555666 ID=cds.MsaT044767.1;Parent=MsaT044767.1
Chr8 exon 64555840 64556144 64555840 ID=MsaT044767.1.exon2;Parent=MsaT044767.1
Chr8 CDS 64555840 64556144 64555840 ID=cds.MsaT044767.1;Parent=MsaT044767.1
Chr8 exon 64556355 64556457 64556355 ID=MsaT044767.1.exon1;Parent=MsaT044767.1
Chr8 CDS 64556355 64556457 64556355 ID=cds.MsaT044767.1;Parent=MsaT044767.1
Gene Sequence

>MsaT044767.1

ATGATGATGGTTGAAAAGGAAGACTTGGGTTTGAGCCTTAGCTTGAACTTCTTTCACAATAACTCTTCTCACAAACCATCTTCCATCCAAACTTTCACTTCAGATCGAAACTTGGAAACATGCAGAGGAGAGAGTGGAAAATACCTACGAGGAATCGACGTGAATCGGTTACCATCTACAGCTATTGAATGCCAAGAGGAAGAAGAAGCAGGGGTTTCATCTCCAAACAGCACAGTTTCAAGTGTTAGTGGAAAAAGAAGCTTGAGAGAAGAAGATCATGACGTGGAAAATAGAGGAAATATCAGTGATGAAGAAGACGCTGAAACTGCTAGGAAAAAACTTAGACTCTCTAAAGACCAATCAGCTATTCTTGAAGAAACTTTCAAAGAACACAATACCCTTAATCCCAAGCAAAAGTTGGCACTTGCAAAGCAACTTGGTCTTCGTCCAAGACAAGTAGAAGTCTGGTTCCAAAACAGAAGAGCTAGGACAAAGCTGAAGCAAACAGAGGTTGATTGTGAAGTGTTGAAAAGGTGCTGTGAAAATCTAACGGAGGAAAATAGGAGACTACAGAAGGAAGTTCAAGAGCTTAGAGCACTGAAACTTTCCCCACAATTTTACATGCAAATGACTCCACCAACTACTCTTACTATGTGCCCTTCTTGTGAACGTGTGGCTGTTCCATCTAATGCTTGTGTTGATGCATCTAACCGTCACCATTCTATGGCCCAAGCCCATCCTCGGGCTGTGCCTATAGGCCCATGGGCTTCTGCTGCTCCAGTCACAAATAGGATGTTTGATGTTTTCCGTCAATGA
Protein sequence

>MsaT044767.1

MMMVEKEDLGLSLSLNFFHNNSSHKPSSIQTFTSDRNLETCRGESGKYLRGIDVNRLPSTAIECQEEEEAGVSSPNSTVSSVSGKRSLREEDHDVENRGNISDEEDAETARKKLRLSKDQSAILEETFKEHNTLNPKQKLALAKQLGLRPRQVEVWFQNRRARTKLKQTEVDCEVLKRCCENLTEENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPSNACVDASNRHHSMAQAHPRAVPIGPWASAAPVTNRMFDVFRQ*