Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044790 | AES90901.1 | 92.570 | 323 | 16 | 5 | 1 | 323 | 1 | 315 | 0.0 | 576 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044790 | sp|Q9M202|ZAT9_ARATH | 35.799 | 338 | 147 | 12 | 1 | 320 | 1 | 286 | 3.01e-46 | 161 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044790 | tr|G7JIV8|G7JIV8_MEDTR | 92.570 | 323 | 16 | 5 | 1 | 323 | 1 | 315 | 0.0 | 576 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG044790 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG044790 | MsaG046618 | 0.805702 | 1.988083e-49 | 3.063748e-47 |
| MsaG044790 | MsaG037387 | 0.810514 | 1.899726e-50 | 3.285126e-48 |
| MsaG016346 | MsaG044790 | 0.803375 | 6.046599e-49 | 8.828283e-47 |
| MsaG027329 | MsaG044790 | 0.800137 | 2.773352e-48 | 3.761302e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044790 | MtrunA17_Chr4g0056091 | 92.570 | 323 | 16 | 5 | 1 | 323 | 1 | 315 | 0.0 | 550 |
| MsaG044790 | MtrunA17_Chr7g0231181 | 40.761 | 368 | 160 | 12 | 1 | 323 | 1 | 355 | 2.85e-66 | 212 |
| MsaG044790 | MtrunA17_Chr5g0399261 | 42.769 | 325 | 103 | 14 | 1 | 308 | 5 | 263 | 8.14e-59 | 190 |
| MsaG044790 | MtrunA17_Chr4g0012691 | 33.967 | 368 | 163 | 9 | 1 | 311 | 2 | 346 | 2.16e-42 | 150 |
| MsaG044790 | MtrunA17_Chr4g0018761 | 26.774 | 310 | 158 | 12 | 6 | 310 | 9 | 254 | 1.55e-17 | 80.9 |
| MsaG044790 | MtrunA17_Chr4g0043071 | 50.000 | 62 | 27 | 1 | 252 | 313 | 441 | 498 | 3.30e-11 | 63.9 |
| MsaG044790 | MtrunA17_Chr1g0207451 | 31.548 | 168 | 75 | 6 | 161 | 313 | 57 | 199 | 5.15e-11 | 62.0 |
| MsaG044790 | MtrunA17_Chr4g0061301 | 50.000 | 64 | 26 | 2 | 253 | 316 | 436 | 493 | 5.31e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG044790 | AT3G60580.1 | 35.799 | 338 | 147 | 12 | 1 | 320 | 1 | 286 | 3.06e-47 | 161 |
| MsaG044790 | AT1G02030.1 | 35.522 | 335 | 129 | 11 | 2 | 320 | 3 | 266 | 3.81e-41 | 144 |
| MsaG044790 | AT2G45120.1 | 34.971 | 346 | 167 | 11 | 1 | 322 | 1 | 312 | 1.77e-40 | 144 |
| MsaG044790 | AT4G16610.1 | 39.306 | 173 | 83 | 5 | 3 | 174 | 4 | 155 | 2.75e-24 | 98.6 |
| MsaG044790 | AT1G26610.1 | 48.000 | 75 | 34 | 2 | 239 | 313 | 386 | 455 | 3.27e-12 | 67.4 |
| MsaG044790 | AT5G61470.1 | 43.902 | 82 | 36 | 3 | 206 | 277 | 218 | 299 | 3.30e-11 | 63.5 |
Find 48 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTGTTGTAGAAAATTC+TGG | 0.230051 | 8:-64884699 | None:intergenic |
| CCAAACACCTTAAAACAAAA+TGG | 0.311153 | 8:-64885090 | None:intergenic |
| TCACGTACAAAAGAAAATTC+AGG | 0.344635 | 8:-64884559 | None:intergenic |
| CACATAGCAACATCTTCTTC+AGG | 0.374474 | 8:-64884805 | None:intergenic |
| GGCACCATGAAACTCGGCTT+TGG | 0.378686 | 8:-64884724 | None:intergenic |
| TCTTTGAATCTAGCAGCAAT+AGG | 0.389886 | 8:-64885204 | None:intergenic |
| GAAACCAAAGCCGAGTTTCA+TGG | 0.392923 | 8:+64884720 | MsaT044790.1:CDS |
| AAGCTTTGTTCAAGGACGTT+TGG | 0.415966 | 8:+64884346 | MsaT044790.1:CDS |
| TCCGATGATGAGAAAGAATC+AGG | 0.418255 | 8:-64884445 | None:intergenic |
| TAGATCGTTGTTGACGAGTC+GGG | 0.431866 | 8:-64884636 | None:intergenic |
| TGATGCTTTCGCGAGACAAA+TGG | 0.443203 | 8:+64884833 | MsaT044790.1:CDS |
| ACCTGATTCTTTCTCATCAT+CGG | 0.450240 | 8:+64884444 | MsaT044790.1:CDS |
| TTGTTGTCGTCGTTGTTGTT+TGG | 0.451043 | 8:-64884673 | None:intergenic |
| GTGAATTGTGATGGAGTTGT+TGG | 0.452696 | 8:-64884745 | None:intergenic |
| GTTTCGATCTTCAAGAGCAT+TGG | 0.459910 | 8:+64884987 | MsaT044790.1:CDS |
| TTTCGATCTTCAAGAGCATT+GGG | 0.469548 | 8:+64884988 | MsaT044790.1:CDS |
| TTTGGTTCTGGTCAAGCACT+TGG | 0.469573 | 8:+64885108 | MsaT044790.1:CDS |
| CTCGTCAACAACGATCTAAA+CGG | 0.483916 | 8:+64884641 | MsaT044790.1:CDS |
| GGTTCTGGTCAAGCACTTGG+TGG | 0.484093 | 8:+64885111 | MsaT044790.1:CDS |
| TGAAGAAGAAGAGGGATCGG+TGG | 0.488736 | 8:+64884894 | MsaT044790.1:CDS |
| TCATCTTCTTCAAGTGGAGC+TGG | 0.500458 | 8:-64885249 | None:intergenic |
| ACAAATGCAAGCTTTGTTCA+AGG | 0.502681 | 8:+64884338 | MsaT044790.1:CDS |
| GCGAAGAATGGTAATGGTAA+TGG | 0.505680 | 8:+64885042 | MsaT044790.1:CDS |
| ACATAGCAACATCTTCTTCA+GGG | 0.525812 | 8:-64884804 | None:intergenic |
| AAGTTATTGAAACGAGTTCG+TGG | 0.531632 | 8:+64884934 | MsaT044790.1:CDS |
| GCAGTGGAAGTTGATGAAGA+AGG | 0.533864 | 8:-64885159 | None:intergenic |
| TGTCGCGATGAAGCGAAGAA+TGG | 0.548591 | 8:+64885030 | MsaT044790.1:CDS |
| TCTTGTTCAGTGAATTGTGA+TGG | 0.549593 | 8:-64884754 | None:intergenic |
| CAGTGGAAGTTGATGAAGAA+GGG | 0.550853 | 8:-64885158 | None:intergenic |
| TTTGGCAATGGAAGAGCACT+TGG | 0.550999 | 8:+64884364 | MsaT044790.1:CDS |
| GTTGTTGGCACCATGAAACT+CGG | 0.554243 | 8:-64884730 | None:intergenic |
| TTAGATCGTTGTTGACGAGT+CGG | 0.558622 | 8:-64884637 | None:intergenic |
| GGAACAAGATGAAGAAGAAG+AGG | 0.558902 | 8:+64884885 | MsaT044790.1:CDS |
| AGATCATCATCTTCTTCAAG+TGG | 0.559232 | 8:-64885255 | None:intergenic |
| GGCAATGGAAGAGCACTTGG+TGG | 0.563472 | 8:+64884367 | MsaT044790.1:CDS |
| TGTTCAAGGACGTTTGGCAA+TGG | 0.572618 | 8:+64884352 | MsaT044790.1:CDS |
| GATGAAGCGAAGAATGGTAA+TGG | 0.574456 | 8:+64885036 | MsaT044790.1:CDS |
| AGCACTTGGTGGACACATGA+AGG | 0.581864 | 8:+64884378 | MsaT044790.1:CDS |
| ATTAACATTAACATTAGCAG+TGG | 0.584695 | 8:-64885175 | None:intergenic |
| AGATGAAGAAGAAGAGGGAT+CGG | 0.594748 | 8:+64884891 | MsaT044790.1:CDS |
| AGTTATTGAAACGAGTTCGT+GGG | 0.596995 | 8:+64884935 | MsaT044790.1:CDS |
| GTATCAGAAACTGAACTCAC+AGG | 0.612015 | 8:-64884778 | None:intergenic |
| CATAGCAACATCTTCTTCAG+GGG | 0.618873 | 8:-64884803 | None:intergenic |
| GAACAAGATGAAGAAGAAGA+GGG | 0.633530 | 8:+64884886 | MsaT044790.1:CDS |
| AGCATTTGTGTGAAAACTGT+GGG | 0.639279 | 8:+64884959 | MsaT044790.1:CDS |
| ATCGGTGGAGAAAATATCGA+AGG | 0.639870 | 8:+64884909 | MsaT044790.1:CDS |
| GATGAACAACATCAACAATG+TGG | 0.658258 | 8:+64884864 | MsaT044790.1:CDS |
| AAGCATTTGTGTGAAAACTG+TGG | 0.714401 | 8:+64884958 | MsaT044790.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 64884328 | 64885299 | 64884328 | ID=MsaG044790 |
| Chr8 | mRNA | 64884328 | 64885299 | 64884328 | ID=MsaT044790.1;Parent=MsaG044790 |
| Chr8 | exon | 64884328 | 64885299 | 64884328 | ID=MsaT044790.1.exon1;Parent=MsaT044790.1 |
| Chr8 | CDS | 64884328 | 64885299 | 64884328 | ID=cds.MsaT044790.1;Parent=MsaT044790.1 |
| Gene Sequence |
| Protein sequence |
