AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044795


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044795 MtrunA17_Chr4g0056091 92.523 321 18 4 1 321 1 315 0.0 551
MsaG044795 MtrunA17_Chr7g0231181 40.984 366 160 12 1 321 1 355 2.09e-66 212
MsaG044795 MtrunA17_Chr5g0399261 43.034 323 103 14 1 306 5 263 1.29e-59 192
MsaG044795 MtrunA17_Chr4g0012691 33.696 368 162 9 1 309 2 346 1.09e-42 151
MsaG044795 MtrunA17_Chr4g0018761 26.948 308 158 12 6 308 9 254 7.35e-18 81.6
MsaG044795 MtrunA17_Chr4g0061301 48.387 62 28 2 253 314 436 493 4.58e-11 63.5
MsaG044795 MtrunA17_Chr1g0152661 27.919 197 103 6 133 307 14 193 5.77e-11 61.6
MsaG044795 MtrunA17_Chr4g0043071 50.000 60 28 1 252 311 441 498 6.67e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044795 AT3G60580.1 35.736 333 152 11 1 318 1 286 7.08e-46 157
MsaG044795 AT1G02030.1 36.036 333 128 11 2 318 3 266 1.81e-41 145
MsaG044795 AT2G45120.1 34.302 344 170 11 1 320 1 312 3.88e-39 140
MsaG044795 AT4G16610.1 39.306 173 83 5 3 174 4 155 4.69e-24 97.8
MsaG044795 AT1G26610.1 46.575 73 36 1 239 311 386 455 1.23e-12 68.6
MsaG044795 AT5G61470.1 43.902 82 36 3 206 277 218 299 3.27e-11 63.5
MsaG044795 AT3G53600.1 36.364 110 55 5 214 312 51 156 4.09e-11 61.2

Find 48 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCTGTTGTAGAAAATTC+TGG 0.230051 8:-64935783 None:intergenic
CCAAACACCTTAAAACAAAA+TGG 0.311153 8:-64936174 None:intergenic
TCACGTACAAAAGAAAATTC+AGG 0.344635 8:-64935643 None:intergenic
CACATAGCAACATCTTCTTC+AGG 0.374474 8:-64935889 None:intergenic
GGCACCATGAAACTCGGCTT+TGG 0.378686 8:-64935808 None:intergenic
TCTTTGAATCTAGCAGCAAT+AGG 0.389886 8:-64936282 None:intergenic
GAAACCAAAGCCGAGTTTCA+TGG 0.392923 8:+64935804 MsaT044795.1:CDS
AAGCTTTGTTCAAGGACGTT+TGG 0.415966 8:+64935430 MsaT044795.1:CDS
TCCGATGATGAGAAAGAATC+AGG 0.418255 8:-64935529 None:intergenic
TAGATCGTTGTTGACGAGTC+GGG 0.431866 8:-64935720 None:intergenic
TGATGCTTTCGCGAGACAAA+TGG 0.443203 8:+64935917 MsaT044795.1:CDS
ACCTGATTCTTTCTCATCAT+CGG 0.450240 8:+64935528 MsaT044795.1:CDS
TTGTTGTCGTCGTTGTTGTT+TGG 0.451043 8:-64935757 None:intergenic
GTGAATTGTGATGGAGTTGT+TGG 0.452696 8:-64935829 None:intergenic
GTTTCGATCTTCAAGAGCAT+TGG 0.459910 8:+64936071 MsaT044795.1:CDS
TTTCGATCTTCAAGAGCATT+GGG 0.469548 8:+64936072 MsaT044795.1:CDS
TTTGGTTCTGGTCAAGCACT+TGG 0.469573 8:+64936192 MsaT044795.1:CDS
CTCGTCAACAACGATCTAAA+CGG 0.483916 8:+64935725 MsaT044795.1:CDS
GGTTCTGGTCAAGCACTTGG+TGG 0.484093 8:+64936195 MsaT044795.1:CDS
TGAAGAAGAAGAGGGATCGG+TGG 0.488736 8:+64935978 MsaT044795.1:CDS
TCATCTTCTTCAAGTGGAGC+TGG 0.500458 8:-64936327 None:intergenic
ACAAATGCAAGCTTTGTTCA+AGG 0.502681 8:+64935422 MsaT044795.1:CDS
GCGAAGAATGGTAATGGTAA+TGG 0.505680 8:+64936126 MsaT044795.1:CDS
ACATAGCAACATCTTCTTCA+GGG 0.525812 8:-64935888 None:intergenic
AAGTTATTGAAACGAGTTCG+TGG 0.531632 8:+64936018 MsaT044795.1:CDS
GCAGTGGAAGTTGATGAAGA+AGG 0.533864 8:-64936243 None:intergenic
TGTCGCGATGAAGCGAAGAA+TGG 0.548591 8:+64936114 MsaT044795.1:CDS
TCTTGTTCAGTGAATTGTGA+TGG 0.549593 8:-64935838 None:intergenic
ATTAATATTAACATTAGCAG+TGG 0.549679 8:-64936259 None:intergenic
CAGTGGAAGTTGATGAAGAA+GGG 0.550853 8:-64936242 None:intergenic
TTTGGCAATGGAAGAGCACT+TGG 0.550999 8:+64935448 MsaT044795.1:CDS
GTTGTTGGCACCATGAAACT+CGG 0.554243 8:-64935814 None:intergenic
TTAGATCGTTGTTGACGAGT+CGG 0.558622 8:-64935721 None:intergenic
GGAACAAGATGAAGAAGAAG+AGG 0.558902 8:+64935969 MsaT044795.1:CDS
AGATCATCATCTTCTTCAAG+TGG 0.559232 8:-64936333 None:intergenic
GGCAATGGAAGAGCACTTGG+TGG 0.563472 8:+64935451 MsaT044795.1:CDS
TGTTCAAGGACGTTTGGCAA+TGG 0.572618 8:+64935436 MsaT044795.1:CDS
GATGAAGCGAAGAATGGTAA+TGG 0.574456 8:+64936120 MsaT044795.1:CDS
AGCACTTGGTGGACACATGA+AGG 0.581864 8:+64935462 MsaT044795.1:CDS
AGATGAAGAAGAAGAGGGAT+CGG 0.594748 8:+64935975 MsaT044795.1:CDS
AGTTATTGAAACGAGTTCGT+GGG 0.596995 8:+64936019 MsaT044795.1:CDS
ATATCAGAAACTGAACTCAC+AGG 0.618063 8:-64935862 None:intergenic
CATAGCAACATCTTCTTCAG+GGG 0.618873 8:-64935887 None:intergenic
GAACAAGATGAAGAAGAAGA+GGG 0.633530 8:+64935970 MsaT044795.1:CDS
AGCATTTGTGTGAAAACTGT+GGG 0.639279 8:+64936043 MsaT044795.1:CDS
ATCGGTGGAGAAAATATCGA+AGG 0.639870 8:+64935993 MsaT044795.1:CDS
GATGAACAACATCAACAATG+TGG 0.658258 8:+64935948 MsaT044795.1:CDS
AAGCATTTGTGTGAAAACTG+TGG 0.714401 8:+64936042 MsaT044795.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 64935412 64936377 64935412 ID=MsaG044795
Chr8 mRNA 64935412 64936377 64935412 ID=MsaT044795.1;Parent=MsaG044795
Chr8 exon 64935412 64936377 64935412 ID=MsaT044795.1.exon1;Parent=MsaT044795.1
Chr8 CDS 64935412 64936377 64935412 ID=cds.MsaT044795.1;Parent=MsaT044795.1
Gene Sequence

>MsaT044795.1

ATGGAAAGACACAAATGCAAGCTTTGTTCAAGGACGTTTGGCAATGGAAGAGCACTTGGTGGACACATGAAGGCTCATCTTGCTATTGCTAAGTCTCAACAGAAACAATTCACTCAACCTGATTCTTTCTCATCATCGGAATCAGAACAAGAAGAAGAAGAAGAAGAAGAAAAAACTTTGATTAATTATGTTTTGAGAGAGAATCCCAAGAAAAGTCTCAAAGTTGCAGATCCTGAATTTTCTTTTGTACGTGAAACTGAGTCTGTTATTGTTCAAGATAGAGAAAGTGAGACTGAGTCAAAGAACAACCCGACTCGTCAACAACGATCTAAACGGAACAGAAAACCAAACAACAACGACGACAACAACAACCAGAATTTTCTACAACAGAAGAAACCAAAGCCGAGTTTCATGGTGCCAACAACTCCATCACAATTCACTGAACAAGAACCTGTGAGTTCAGTTTCTGATATTTCCCCTGAAGAAGATGTTGCTATGTGTCTCATGATGCTTTCGCGAGACAAATGGAGTAGAAAGATGAACAACATCAACAATGTGGAACAAGATGAAGAAGAAGAGGGATCGGTGGAGAAAATATCGAAGGTGAAGTTATTGAAACGAGTTCGTGGGAAGCATTTGTGTGAAAACTGTGGGAAGACGTTTCGATCTTCAAGAGCATTGGGAAGTCATAGAAGTATTTGTTGTCGCGATGAAGCGAAGAATGGTAATGGTAATGGTAAAGATGATAAGATTTTTGAATGTCCATTTTGTTTTAAGGTGTTTGGTTCTGGTCAAGCACTTGGTGGTCACAAGAGATCTCATCTCATGATCCCTTCTTCATCAACTTCCACTGCTAATGTTAATATTAATCCTATTGCTGCTAGATTCAAAGAGAGTTTCATAGATCTCAATATGCCAGCTCCACTTGAAGAAGATGATGATCTTAGTGTTGTTTCTGATGCCTAA

Protein sequence

>MsaT044795.1

MERHKCKLCSRTFGNGRALGGHMKAHLAIAKSQQKQFTQPDSFSSSESEQEEEEEEEKTLINYVLRENPKKSLKVADPEFSFVRETESVIVQDRESETESKNNPTRQQRSKRNRKPNNNDDNNNQNFLQQKKPKPSFMVPTTPSQFTEQEPVSSVSDISPEEDVAMCLMMLSRDKWSRKMNNINNVEQDEEEEGSVEKISKVKLLKRVRGKHLCENCGKTFRSSRALGSHRSICCRDEAKNGNGNGKDDKIFECPFCFKVFGSGQALGGHKRSHLMIPSSSTSTANVNINPIAARFKESFIDLNMPAPLEEDDDLSVVSDA*