AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044830 MtrunA17_Chr4g0056521 99.083 218 2 0 1 218 1 218 6.85e-164 450
MsaG044830 MtrunA17_Chr4g0011841 71.782 202 49 6 20 218 25 221 1.04e-90 265
MsaG044830 MtrunA17_Chr2g0322221 49.268 205 89 6 8 208 2 195 5.92e-56 176
MsaG044830 MtrunA17_Chr4g0063931 53.631 179 64 5 7 181 2 165 2.62e-55 174
MsaG044830 MtrunA17_Chr4g0025361 49.254 201 77 7 9 208 1 177 6.02e-50 160
MsaG044830 MtrunA17_Chr5g0413381 50.932 161 65 5 18 174 12 162 9.52e-46 150
MsaG044830 MtrunA17_Chr5g0419871 43.713 167 74 4 9 173 7 155 4.93e-34 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044830 AT2G45190.1 61.468 218 72 7 10 218 15 229 2.50e-82 245
MsaG044830 AT4G00180.1 61.778 225 63 9 12 216 17 238 2.15e-75 228
MsaG044830 AT4G00180.2 60.476 210 60 9 27 216 1 207 5.39e-64 197
MsaG044830 AT2G26580.2 56.970 165 53 4 17 181 8 154 2.19e-57 179
MsaG044830 AT2G26580.1 56.970 165 53 4 17 181 8 154 2.19e-57 179
MsaG044830 AT1G08465.1 51.031 194 77 5 17 208 7 184 3.08e-54 172
MsaG044830 AT2G45190.2 57.738 168 59 7 60 218 1 165 3.16e-54 171
MsaG044830 AT1G23420.1 46.286 175 89 4 6 177 8 180 1.64e-37 130
MsaG044830 AT1G23420.2 46.286 175 89 4 6 177 39 211 8.45e-37 129
MsaG044830 AT1G69180.1 45.283 159 69 3 15 173 16 156 2.59e-34 121

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGGTTCATCATAAAATT+TGG 0.162315 8:+65394680 None:intergenic
AATCATTTGGTGCATTTATA+TGG 0.220470 8:+65394663 None:intergenic
CCAGAAGGTTATGATGATTA+GGG 0.334302 8:+65394857 None:intergenic
ATAAAATTTGGAGCTGGGTT+TGG 0.340235 8:+65394692 None:intergenic
GCTTCCCTGTGGGTTATATC+AGG 0.345088 8:+65393434 None:intergenic
CCCTAATCATCATAACCTTC+TGG 0.353222 8:-65394857 MsaT044830.1:intron
ACCAGAAGGTTATGATGATT+AGG 0.354968 8:+65394856 None:intergenic
GTCTTCTTCATAGTCTGATC+AGG 0.388325 8:+65392570 None:intergenic
ATAGAGATTGATGCATTAGT+AGG 0.390762 8:+65392326 None:intergenic
CACTTCCCACACATTCACTT+TGG 0.432093 8:-65392600 MsaT044830.1:CDS
TGTCCCAAATGAAACTGATT+GGG 0.435332 8:+65394892 None:intergenic
TTCAACCACAACATTATCCT+TGG 0.449974 8:-65395200 MsaT044830.1:CDS
GTGTCCCAAATGAAACTGAT+TGG 0.468609 8:+65394891 None:intergenic
AAAGGAGGATCATACATTCT+TGG 0.469847 8:+65393397 None:intergenic
AGTAATGCACGCATGTTGAC+TGG 0.472196 8:+65394925 None:intergenic
TTCATCATAAAATTTGGAGC+TGG 0.473136 8:+65394686 None:intergenic
GGAGGCATAGAGAAATCATT+TGG 0.480020 8:+65394650 None:intergenic
TCACGTCTGTTTATGATTGG+TGG 0.480315 8:+65394596 None:intergenic
TCATCATAAAATTTGGAGCT+GGG 0.482099 8:+65394687 None:intergenic
ATCTCACGTCTGTTTATGAT+TGG 0.489279 8:+65394593 None:intergenic
ATTCTTGGCAGCTGCGCTGA+AGG 0.503305 8:+65393412 None:intergenic
CAAAGTGAATGTGTGGGAAG+TGG 0.524635 8:+65392601 None:intergenic
CTGTTTATGATTGGTGGTCG+TGG 0.531680 8:+65394602 None:intergenic
TGCGCTGAAGGCTTCCCTGT+GGG 0.539083 8:+65393424 None:intergenic
CAGATTCTGATGAAAGATGC+GGG 0.541160 8:-65392381 MsaT044830.1:CDS
AACAAAGTTGCTCGGAAGGA+GGG 0.547749 8:+65395170 None:intergenic
GGAAGGAGGGAGGTGGTCCA+AGG 0.549892 8:+65395183 None:intergenic
GAAGACAAATGTGTGCCAGC+AGG 0.553012 8:-65392553 MsaT044830.1:intron
CTGCGCTGAAGGCTTCCCTG+TGG 0.555021 8:+65393423 None:intergenic
GATGTTTCATACAGCAAGGA+TGG 0.571365 8:+65395123 None:intergenic
TAGAGATTGATGCATTAGTA+GGG 0.577948 8:+65392327 None:intergenic
GGATAATGTTGTGGTTGAAG+AGG 0.579637 8:+65395204 None:intergenic
GTCTTGAACAAGCTTGTGCA+AGG 0.580384 8:+65394985 None:intergenic
TAACAAAGTTGCTCGGAAGG+AGG 0.582448 8:+65395169 None:intergenic
GTTGCTCGGAAGGAGGGAGG+TGG 0.583306 8:+65395176 None:intergenic
GATTGACAGACTTAATGCGT+TGG 0.585391 8:+65393456 None:intergenic
ACATAACAAAGTTGCTCGGA+AGG 0.586092 8:+65395166 None:intergenic
AAAGTGAATGTGTGGGAAGT+GGG 0.594462 8:+65392602 None:intergenic
CTCATCAGCTACAGTCCTAG+TGG 0.598065 8:+65394628 None:intergenic
AGCAAGGATGGTGTCACAGA+TGG 0.603099 8:+65395135 None:intergenic
TCAATCCTGATATAACCCAC+AGG 0.603889 8:-65393439 MsaT044830.1:CDS
TCATCAGCTACAGTCCTAGT+GGG 0.606377 8:+65394629 None:intergenic
ATTTCTCTATGCCTCCCACT+AGG 0.608537 8:-65394643 MsaT044830.1:CDS
TCAGATTCTGATGAAAGATG+CGG 0.615278 8:-65392382 MsaT044830.1:CDS
GTGGACATAACAAAGTTGCT+CGG 0.624197 8:+65395162 None:intergenic
ATGAGACCAAAGTGAATGTG+TGG 0.636661 8:+65392594 None:intergenic
GTCCCAAATGAAACTGATTG+GGG 0.640416 8:+65394893 None:intergenic
CAATCCTGATATAACCCACA+GGG 0.644329 8:-65393438 MsaT044830.1:CDS
CAGAAGGTTATGATGATTAG+GGG 0.656738 8:+65394858 None:intergenic
AAAGTTGCTCGGAAGGAGGG+AGG 0.666622 8:+65395173 None:intergenic
ATGAAGCGATTGTATGCTGA+GGG 0.673566 8:+65393625 None:intergenic
TCAGCTACAGTCCTAGTGGG+AGG 0.678197 8:+65394632 None:intergenic
TGGTGTCACAGATGGTGCAG+TGG 0.683501 8:+65395143 None:intergenic
AAGACTGTTACTGTGAGATG+CGG 0.688688 8:-65394967 MsaT044830.1:CDS
TGAGACCAAAGTGAATGTGT+GGG 0.708380 8:+65392595 None:intergenic
GTGGTCCAAGGATAATGTTG+TGG 0.713676 8:+65395195 None:intergenic
GATGAAGCGATTGTATGCTG+AGG 0.735800 8:+65393624 None:intergenic
TCCCAAATGAAACTGATTGG+GGG 0.774348 8:+65394894 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 65392341 65395233 65392341 ID=MsaG044830
Chr8 mRNA 65392341 65395233 65392341 ID=MsaT044830.1;Parent=MsaG044830
Chr8 exon 65392341 65392415 65392341 ID=MsaT044830.1.exon7;Parent=MsaT044830.1
Chr8 CDS 65392341 65392415 65392341 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Chr8 exon 65392554 65392628 65392554 ID=MsaT044830.1.exon6;Parent=MsaT044830.1
Chr8 CDS 65392554 65392628 65392554 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Chr8 exon 65393412 65393487 65393412 ID=MsaT044830.1.exon5;Parent=MsaT044830.1
Chr8 CDS 65393412 65393487 65393412 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Chr8 exon 65393622 65393670 65393622 ID=MsaT044830.1.exon4;Parent=MsaT044830.1
Chr8 CDS 65393622 65393670 65393622 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Chr8 exon 65394600 65394723 65394600 ID=MsaT044830.1.exon3;Parent=MsaT044830.1
Chr8 CDS 65394600 65394723 65394600 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Chr8 exon 65394858 65395016 65394858 ID=MsaT044830.1.exon2;Parent=MsaT044830.1
Chr8 CDS 65394858 65395016 65394858 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Chr8 exon 65395135 65395233 65395135 ID=MsaT044830.1.exon1;Parent=MsaT044830.1
Chr8 CDS 65395135 65395233 65395135 ID=cds.MsaT044830.1;Parent=MsaT044830.1
Gene Sequence

>MsaT044830.1

ATGTCGTCCTCTTCAACCACAACATTATCCTTGGACCACCTCCCTCCTTCCGAGCAACTTTGTTATGTCCACTGCACCATCTGTGACACCATCCTTGCTGTGAGTGTTCCTTGCACAAGCTTGTTCAAGACTGTTACTGTGAGATGCGGTCATTGCACTAATCTGCTTCCAGTCAACATGCGTGCATTACTTCTGCCATCCCCCAATCAGTTTCATTTGGGACACTCTTTCTTCTCCCCTAATCATCATAACCTTCTGGAGGAGATGCCAAACCCAGCTCCAAATTTTATGATGAACCATATAAATGCACCAAATGATTTCTCTATGCCTCCCACTAGGACTGTAGCTGATGAGCTTCCACGACCACCAATCATAAACAGACCTCCAGAGAAAAGACAGAGAGTTCCCTCAGCATACAATCGCTTCATCAAGGACGAGATCCAACGCATTAAGTCTGTCAATCCTGATATAACCCACAGGGAAGCCTTCAGCGCAGCTGCCAAGAATTGGGCCCACTTCCCACACATTCACTTTGGTCTCATGCCTGATCAGACTATGAAGAAGACAAATGTGTGCCAGCAGGAAGGTGATGATCAGATTCTGATGAAAGATGCGGGATTTTATGCTTCTGCTAATCTTGGTGTTTCGCCCTACTAA
Protein sequence

>MsaT044830.1

MSSSSTTTLSLDHLPPSEQLCYVHCTICDTILAVSVPCTSLFKTVTVRCGHCTNLLPVNMRALLLPSPNQFHLGHSFFSPNHHNLLEEMPNPAPNFMMNHINAPNDFSMPPTRTVADELPRPPIINRPPEKRQRVPSAYNRFIKDEIQRIKSVNPDITHREAFSAAAKNWAHFPHIHFGLMPDQTMKKTNVCQQEGDDQILMKDAGFYASANLGVSPY*