AlfalfaGEDB | MsaG044890

M. sativa ssp. caerulea / MsaG044890

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009966 GO:0009967 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1900457 GO:1900459 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03514.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044890	XP_024638226.1	90.869	679	8	3	1	627	1	677	0.0	1245

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044890	sp\|Q9CAN3\|SCL28_ARATH	50.857	700	210	22	1	612	1	654	0.0	590

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044890	tr\|A0A072URL3\|A0A072URL3_MEDTR	90.869	679	8	3	1	627	1	677	0.0	1245

TFs/TRs:

Gene ID	Type	Classification
MsaG044890	TF	GRAS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG001936	MsaG044890	0.801713	1.325904e-48	1.863494e-46
MsaG003646	MsaG044890	0.818242	3.786514e-52	7.952666e-50
MsaG004339	MsaG044890	0.861091	2.580728e-63	2.091488e-60
MsaG004466	MsaG044890	0.826723	4.133509e-54	1.090673e-51
MsaG006131	MsaG044890	0.818700	2.984706e-52	6.343611e-50
MsaG006186	MsaG044890	0.829784	7.618540e-55	2.191232e-52
MsaG006312	MsaG044890	0.805390	2.310616e-49	3.534875e-47
MsaG006544	MsaG044890	0.812181	8.288308e-51	1.493018e-48
MsaG006831	MsaG044890	0.831833	2.408838e-55	7.350569e-53
MsaG009270	MsaG044890	0.802023	1.146033e-48	1.622058e-46
MsaG010316	MsaG044890	0.835700	2.629044e-56	8.992512e-54
MsaG010544	MsaG044890	0.803192	6.594373e-49	9.587608e-47
MsaG011119	MsaG044890	0.849501	5.900694e-60	3.144428e-57
MsaG012161	MsaG044890	0.822586	3.857915e-53	9.088837e-51
MsaG012360	MsaG044890	0.813818	3.642152e-51	6.834683e-49
MsaG044267	MsaG044890	0.800212	2.677645e-48	3.637629e-46
MsaG044277	MsaG044890	0.802311	1.000406e-48	1.425373e-46
MsaG044890	MsaG045542	0.816628	8.707157e-52	1.754228e-49
MsaG044890	MsaG045647	0.806000	1.722934e-49	2.673640e-47
MsaG044890	MsaG046035	0.804215	4.053841e-49	6.035151e-47
MsaG044890	MsaG047065	0.815680	1.414674e-51	2.782242e-49
MsaG044890	MsaG001319	0.830134	6.262866e-55	1.819527e-52
MsaG044890	MsaG010803	0.860299	4.474977e-63	3.520026e-60
MsaG044890	MsaG033667	0.829519	8.831897e-55	2.521022e-52
MsaG044890	MsaG036611	0.803281	6.321528e-49	9.209727e-47
MsaG044890	MsaG036557	0.852093	1.108396e-60	6.460182e-58
MsaG044890	MsaG043567	0.816918	7.500966e-52	1.522641e-49
MsaG012887	MsaG044890	0.800200	2.693422e-48	3.658017e-46
MsaG013633	MsaG044890	0.813028	5.422312e-51	9.975802e-49
MsaG014101	MsaG044890	0.837283	1.044297e-56	3.746656e-54
MsaG014232	MsaG044890	0.842344	5.093777e-58	2.141710e-55
MsaG014630	MsaG044890	0.802334	9.898062e-49	1.411002e-46
MsaG014642	MsaG044890	0.802983	7.283142e-49	1.053774e-46
MsaG016687	MsaG044890	0.800133	2.778271e-48	3.767653e-46
MsaG017919	MsaG044890	0.815882	1.276057e-51	2.522463e-49
MsaG018539	MsaG044890	0.825273	9.103418e-54	2.307172e-51
MsaG019211	MsaG044890	0.802803	7.930163e-49	1.142632e-46
MsaG019641	MsaG044890	0.810800	1.648718e-50	2.871014e-48
MsaG019669	MsaG044890	0.844469	1.388144e-58	6.250243e-56
MsaG019945	MsaG044890	0.800018	2.931926e-48	3.965820e-46
MsaG020042	MsaG044890	0.820750	1.020452e-52	2.289248e-50
MsaG021109	MsaG044890	0.807039	1.042144e-49	1.657455e-47
MsaG022433	MsaG044890	0.800868	1.971317e-48	2.718056e-46
MsaG024000	MsaG044890	0.845222	8.713093e-59	4.021708e-56
MsaG027021	MsaG044890	0.804742	3.152007e-49	4.750129e-47
MsaG027217	MsaG044890	0.831925	2.287551e-55	6.998940e-53
MsaG032127	MsaG044890	0.821160	8.220978e-53	1.864517e-50
MsaG033936	MsaG044890	0.800306	2.563764e-48	3.490289e-46
MsaG033931	MsaG044890	0.802653	8.512471e-49	1.222366e-46
MsaG033932	MsaG044890	0.854002	3.169843e-61	1.976291e-58
MsaG034399	MsaG044890	0.807150	9.878796e-50	1.575247e-47
MsaG035157	MsaG044890	0.804516	3.511444e-49	5.264130e-47
MsaG035658	MsaG044890	0.827047	3.460663e-54	9.214034e-52
MsaG037261	MsaG044890	0.811639	1.086412e-50	1.931077e-48
MsaG037339	MsaG044890	0.824802	1.174524e-53	2.938549e-51
MsaG039483	MsaG044890	0.804881	2.949706e-49	4.459633e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044890	MtrunA17_Chr4g0057291	90.869	679	8	3	1	627	1	677	0.0	1245
MsaG044890	MtrunA17_Chr7g0224271	42.761	297	159	5	316	611	386	672	2.20e-72	246
MsaG044890	MtrunA17_Chr2g0306641	41.275	298	158	7	326	614	200	489	1.11e-63	218
MsaG044890	MtrunA17_Chr8g0376381	39.661	295	159	10	326	611	237	521	2.96e-56	199
MsaG044890	MtrunA17_Chr7g0245601	38.538	301	172	8	314	613	505	793	3.10e-53	195
MsaG044890	MtrunA17_Chr5g0445331	35.276	326	183	8	297	611	223	531	2.12e-51	186
MsaG044890	MtrunA17_Chr3g0125811	39.216	306	164	13	318	611	274	569	8.17e-51	185
MsaG044890	MtrunA17_Chr4g0038761	38.870	301	166	10	320	616	160	446	3.04e-50	181
MsaG044890	MtrunA17_Chr4g0057891	35.404	322	190	11	299	611	207	519	9.93e-50	181
MsaG044890	MtrunA17_Chr3g0110971	35.762	302	169	7	328	611	292	586	1.30e-49	182
MsaG044890	MtrunA17_Chr1g0200401	36.646	322	169	12	315	611	147	458	4.00e-49	177
MsaG044890	MtrunA17_Chr1g0159461	37.662	308	170	12	316	611	294	591	4.29e-49	180
MsaG044890	MtrunA17_Chr5g0447691	36.393	305	178	9	316	611	246	543	1.66e-47	175
MsaG044890	MtrunA17_Chr8g0357601	34.853	307	185	8	311	611	254	551	2.76e-47	175
MsaG044890	MtrunA17_Chr1g0183641	36.184	304	182	7	311	612	184	477	3.17e-47	173
MsaG044890	MtrunA17_Chr2g0319091	34.810	316	180	10	310	611	275	578	3.77e-47	174
MsaG044890	MtrunA17_Chr3g0101141	33.974	312	178	9	315	611	243	541	1.04e-43	164
MsaG044890	MtrunA17_Chr4g0076091	33.115	305	188	7	316	611	245	542	4.56e-43	162
MsaG044890	MtrunA17_Chr4g0039661	33.333	306	185	10	315	611	259	554	9.48e-43	162
MsaG044890	MtrunA17_Chr2g0328371	32.143	252	163	5	318	563	2	251	1.95e-41	150
MsaG044890	MtrunA17_Chr2g0290241	31.475	305	193	8	316	611	127	424	2.75e-40	152
MsaG044890	MtrunA17_Chr2g0328391	32.248	307	190	9	318	613	371	670	4.36e-40	155
MsaG044890	MtrunA17_Chr3g0085681	27.297	381	211	7	284	611	68	435	5.19e-39	149
MsaG044890	MtrunA17_Chr2g0328341	28.431	306	204	7	316	613	341	639	1.61e-38	150
MsaG044890	MtrunA17_Chr3g0114841	33.654	312	183	11	313	612	201	500	6.20e-38	147
MsaG044890	MtrunA17_Chr4g0038851	28.099	363	194	8	310	611	112	468	4.55e-37	144
MsaG044890	MtrunA17_Chr3g0085361	27.874	348	191	5	316	611	10	349	1.56e-36	140
MsaG044890	MtrunA17_Chr2g0328381	28.247	308	202	8	316	613	344	642	2.19e-36	144
MsaG044890	MtrunA17_Chr4g0031711	30.000	310	199	9	316	614	433	735	3.21e-36	144
MsaG044890	MtrunA17_Chr4g0031721	30.293	307	199	8	316	613	385	685	3.23e-36	144
MsaG044890	MtrunA17_Chr4g0031701	29.831	295	191	7	329	613	339	627	1.29e-35	142
MsaG044890	MtrunA17_Chr4g0031731	30.405	296	188	8	329	613	339	627	8.32e-35	139
MsaG044890	MtrunA17_Chr5g0424131	32.803	314	186	11	316	612	194	499	3.46e-34	136
MsaG044890	MtrunA17_Chr7g0238891	27.541	305	206	6	318	613	443	741	1.75e-33	136
MsaG044890	MtrunA17_Chr2g0294461	30.333	300	194	8	320	611	292	584	1.04e-32	133
MsaG044890	MtrunA17_Chr7g0270031	28.956	297	192	8	323	611	268	553	1.59e-32	132
MsaG044890	MtrunA17_Chr5g0401131	28.329	353	227	11	280	613	146	491	1.65e-32	131
MsaG044890	MtrunA17_Chr7g0236851	34.221	263	160	10	354	611	402	656	1.45e-31	130
MsaG044890	MtrunA17_Chr4g0031741	26.384	307	212	7	316	613	350	651	1.53e-31	130
MsaG044890	MtrunA17_Chr8g0383981	30.671	313	183	13	317	615	207	499	1.86e-31	128
MsaG044890	MtrunA17_Chr1g0194621	26.298	289	203	7	326	611	236	517	4.23e-31	127
MsaG044890	MtrunA17_Chr2g0328401	28.185	259	173	6	311	559	351	606	1.24e-30	127
MsaG044890	MtrunA17_Chr2g0294471	29.553	291	189	9	329	611	293	575	1.73e-30	126
MsaG044890	MtrunA17_Chr2g0328411	25.490	306	211	7	316	611	355	653	3.02e-30	126
MsaG044890	MtrunA17_Chr2g0328431	25.649	308	212	8	316	613	355	655	1.06e-28	121
MsaG044890	MtrunA17_Chr6g0468561	32.222	270	162	10	354	611	401	661	8.61e-26	112
MsaG044890	MtrunA17_Chr5g0396491	34.286	140	92	0	310	449	120	259	1.31e-23	104
MsaG044890	MtrunA17_Chr5g0396491	33.333	153	95	5	460	611	331	477	4.80e-19	90.5
MsaG044890	MtrunA17_Chr2g0328361	24.918	305	183	7	320	613	347	616	1.44e-23	105
MsaG044890	MtrunA17_Chr2g0329591	25.633	316	192	7	324	613	154	452	2.16e-23	103
MsaG044890	MtrunA17_Chr4g0012071	26.557	305	195	10	318	611	264	550	8.03e-23	102
MsaG044890	MtrunA17_Chr5g0401421	24.390	328	200	10	327	613	127	447	5.16e-22	99.4
MsaG044890	MtrunA17_Chr7g0230771	26.333	300	201	8	318	611	445	730	1.45e-20	96.3
MsaG044890	MtrunA17_Chr7g0230731	25.753	299	204	6	318	611	442	727	4.57e-20	94.7
MsaG044890	MtrunA17_Chr8g0373561	28.526	312	195	12	311	611	246	540	5.70e-17	84.3
MsaG044890	MtrunA17_Chr4g0053901	22.832	346	213	12	314	613	132	469	7.23e-15	77.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG044890	AT1G63100.2	50.857	700	210	22	1	612	1	654	0.0	590
MsaG044890	AT1G63100.1	48.857	700	199	20	1	612	1	629	0.0	560
MsaG044890	AT1G66350.1	40.210	286	157	9	329	611	231	505	3.07e-57	201
MsaG044890	AT1G14920.1	39.116	294	161	10	326	611	245	528	4.65e-55	196
MsaG044890	AT3G54220.1	35.826	321	195	6	293	613	342	651	1.35e-54	197
MsaG044890	AT2G01570.1	39.116	294	161	9	326	611	297	580	3.82e-54	194
MsaG044890	AT3G03450.1	37.586	290	166	9	329	611	263	544	4.13e-52	188
MsaG044890	AT1G55580.1	39.934	303	160	9	324	611	149	444	4.14e-51	183
MsaG044890	AT5G41920.1	38.235	306	167	12	311	611	111	399	1.37e-49	178
MsaG044890	AT5G17490.1	36.806	288	169	9	329	611	236	515	8.85e-49	178
MsaG044890	AT1G50420.1	31.117	376	190	9	296	611	112	478	6.21e-47	172
MsaG044890	AT5G66770.1	33.981	309	184	10	315	611	283	583	2.13e-46	173
MsaG044890	AT5G48150.1	35.831	307	177	11	316	611	192	489	1.45e-44	166
MsaG044890	AT5G48150.4	35.831	307	177	11	316	611	192	489	1.45e-44	166
MsaG044890	AT5G48150.2	35.831	307	177	11	316	611	192	489	1.45e-44	166
MsaG044890	AT5G48150.3	35.831	307	177	11	316	611	192	489	1.45e-44	166
MsaG044890	AT4G17230.1	35.179	307	183	8	316	613	227	526	1.61e-42	161
MsaG044890	AT4G17230.2	35.179	307	183	8	316	613	227	526	1.61e-42	161
MsaG044890	AT2G04890.1	35.000	300	182	10	316	611	122	412	1.97e-42	158
MsaG044890	AT1G50600.3	35.621	306	179	10	316	611	228	525	2.09e-42	160
MsaG044890	AT1G50600.1	35.621	306	179	10	316	611	299	596	4.69e-42	161
MsaG044890	AT1G50600.2	35.621	306	179	10	316	611	303	600	5.90e-42	160
MsaG044890	AT3G50650.1	33.968	315	180	12	315	611	237	541	3.37e-41	157
MsaG044890	AT1G21450.1	34.853	307	183	9	315	611	293	592	5.73e-41	157
MsaG044890	AT2G29065.1	32.372	312	189	11	316	613	326	629	3.63e-38	150
MsaG044890	AT2G29060.2	31.511	311	195	10	316	614	353	657	4.49e-36	144
MsaG044890	AT2G29060.1	31.511	311	195	10	316	614	390	694	5.71e-36	144
MsaG044890	AT1G07530.1	30.519	308	198	9	316	613	465	766	4.60e-35	142
MsaG044890	AT1G07520.2	32.226	301	180	14	328	613	239	530	1.05e-34	139
MsaG044890	AT1G07520.4	32.226	301	180	14	328	613	239	530	1.05e-34	139
MsaG044890	AT1G07520.1	32.226	301	180	14	328	613	403	694	2.60e-34	139
MsaG044890	AT1G07520.3	32.226	301	180	14	328	613	404	695	2.61e-34	139
MsaG044890	AT4G08250.1	29.560	318	196	11	309	611	172	476	4.22e-33	133
MsaG044890	AT5G59450.1	26.774	310	212	7	316	615	297	601	1.42e-32	133
MsaG044890	AT3G46600.2	28.339	307	205	7	316	613	72	372	3.62e-32	128
MsaG044890	AT4G37650.1	29.355	310	192	9	324	613	228	530	4.21e-32	131
MsaG044890	AT3G46600.1	28.339	307	205	7	316	613	280	580	6.50e-32	131
MsaG044890	AT3G46600.3	28.339	307	205	7	316	613	248	548	1.83e-31	129
MsaG044890	AT2G37650.2	27.476	313	196	9	317	613	405	702	1.25e-29	125
MsaG044890	AT2G37650.1	27.476	313	196	9	317	613	417	714	1.55e-29	124
MsaG044890	AT5G52510.1	29.283	321	193	10	316	611	328	639	2.60e-27	117
MsaG044890	AT3G60630.1	27.597	308	196	12	318	611	327	621	7.03e-24	107
MsaG044890	AT4G00150.1	29.000	300	188	11	318	611	273	553	5.78e-23	103
MsaG044890	AT2G45160.1	27.302	315	185	11	318	611	347	638	1.47e-21	99.8
MsaG044890	AT3G49950.1	26.730	318	203	10	317	614	103	410	1.87e-20	94.7

Find 156 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTTCTCTTTGAATTGTTC+TGG	0.202054	8:-66143272	MsaT044890.1:CDS
ACTGGTTGTGTTGATGCTAT+TGG	0.221753	8:-66142918	MsaT044890.1:CDS
GTAACAAAGATCACCAATTT+TGG	0.231391	8:-66143164	MsaT044890.1:CDS
AGATCAAGGATGAGGAGTTT+TGG	0.247813	8:-66143505	MsaT044890.1:CDS
AGTTTCAGTTTGGCACAATT+TGG	0.253074	8:-66143356	MsaT044890.1:CDS
TTGGAGAGGCACCAGAGTTT+TGG	0.253860	8:-66142035	MsaT044890.1:CDS
CTCTCTACACAGTATCTTCT+TGG	0.270369	8:-66141839	MsaT044890.1:CDS
TGTCACCAACTCACAGATTA+AGG	0.272810	8:-66143769	MsaT044890.1:CDS
CTGCTGTTGTTCTTGACTTC+AGG	0.279004	8:+66143596	None:intergenic
CTGGTTGTGTTGATGCTATT+GGG	0.293519	8:-66142917	MsaT044890.1:intron
AAATAGAGCTAAGAGGAAAA+AGG	0.307901	8:-66143420	MsaT044890.1:CDS
GCTTGCAGAACATGGATCTT+TGG	0.333074	8:-66143456	MsaT044890.1:CDS
CAATCCTACAATTGTTGTTA+TGG	0.340164	8:-66142249	MsaT044890.1:CDS
TGGACACCGGTTGACGTTGC+TGG	0.348429	8:-66141819	MsaT044890.1:CDS
CATGAAGAGGGTAATGGTTC+GGG	0.351755	8:-66143032	MsaT044890.1:CDS
TGCCTTTGTCTAACAATCCT+TGG	0.358700	8:-66143199	MsaT044890.1:CDS
AATACATCTTCAACAGAAAC+TGG	0.362646	8:+66141937	None:intergenic
TCAGTTCCTTCATGCTGATA+AGG	0.365523	8:+66143005	None:intergenic
CGATGTGAAATGAAGAAACT+TGG	0.373450	8:+66142658	None:intergenic
TCTGCCATAACAACAATTGT+AGG	0.376086	8:+66142245	None:intergenic
GTGGCTACCCTCACCAAAAT+TGG	0.379531	8:+66143151	None:intergenic
AATTTGGAGCTGGAAACTTT+TGG	0.382164	8:-66143340	MsaT044890.1:CDS
TGAAAGGGAAAGTTTCAGTT+TGG	0.386013	8:-66143366	MsaT044890.1:CDS
AATCTCTGTGTGCTCATTGA+TGG	0.390998	8:+66143704	None:intergenic
GGAGCAACAGCACTTACTTT+GGG	0.396486	8:-66141876	MsaT044890.1:CDS
GATGCATGGGAGTCACTGAT+AGG	0.410356	8:-66141974	MsaT044890.1:CDS
ATCAATGAGCACACAGAGAT+TGG	0.410565	8:-66143702	MsaT044890.1:CDS
GACTTGAAGATGTAAGGCTT+TGG	0.411643	8:-66142391	MsaT044890.1:CDS
TGTTCCAAAGTTTAGCTTCA+AGG	0.419556	8:-66142547	MsaT044890.1:CDS
AGCAACAACTGCTCAGACAT+TGG	0.422074	8:-66143531	MsaT044890.1:CDS
TCTTCAAGTCTGTCGACTAC+TGG	0.429762	8:+66142404	None:intergenic
TCGAACATGGCTTGGAGGAT+TGG	0.430304	8:+66142523	None:intergenic
ACATGAAGAGGGTAATGGTT+CGG	0.431411	8:-66143033	MsaT044890.1:CDS
TTCGCAGCAGCCGTTGAGGC+CGG	0.436828	8:-66143639	MsaT044890.1:CDS
AGGAGCAACAGCACTTACTT+TGG	0.437760	8:-66141877	MsaT044890.1:CDS
AGAGTCAGAGTTTGAGCCTA+AGG	0.440522	8:-66143076	MsaT044890.1:CDS
AAATGAAGAAACTTGGGAAT+TGG	0.443639	8:+66142665	None:intergenic
GCAACAACTGCTCAGACATT+GGG	0.444891	8:-66143530	MsaT044890.1:CDS
AGATGTTAGCTAGGGAGATA+AGG	0.446179	8:-66142088	MsaT044890.1:CDS
AAGCAAGAGCTGAATGAAAC+CGG	0.462075	8:-66142479	MsaT044890.1:CDS
TGGCCCTTGAAGCTAAACTT+TGG	0.467104	8:+66142543	None:intergenic
CCAGAACAATTCAAAGAGAA+AGG	0.469406	8:+66143272	None:intergenic
GAGCTTCCAGCAACGTCAAC+CGG	0.470552	8:+66141813	None:intergenic
GTTCTCTGCAGCAGAACATT+CGG	0.471882	8:-66143565	MsaT044890.1:CDS
TTGTTCTGGAGATGAGGAAA+GGG	0.476638	8:-66143258	MsaT044890.1:CDS
GAAACCGGAGACAGACTTGC+TGG	0.481646	8:-66142464	MsaT044890.1:CDS
CATAACAACAATTGTAGGAT+TGG	0.483005	8:+66142250	None:intergenic
AGATAGAGGAGATGTTAGCT+AGG	0.487005	8:-66142097	MsaT044890.1:CDS
CCAAGCCATGTTCGAATCAC+GGG	0.489720	8:-66142515	MsaT044890.1:CDS
TGATCACAGTGGCCTTCCAC+TGG	0.491665	8:-66142138	MsaT044890.1:CDS
TCAGACATTGGGAGAGATCA+AGG	0.493655	8:-66143519	MsaT044890.1:CDS
CCATGTTCGAATCACGGGTG+TGG	0.493749	8:-66142510	MsaT044890.1:CDS
TCCGATGAGGATCAAGATAG+AGG	0.495969	8:-66142111	MsaT044890.1:CDS
TGCATTGAGGCTTCTAAACC+AGG	0.497162	8:-66142693	MsaT044890.1:CDS
CACAGTATCTTCTTGGACAC+CGG	0.497435	8:-66141832	MsaT044890.1:CDS
CATGTTCGAATCACGGGTGT+GGG	0.498098	8:-66142509	MsaT044890.1:CDS
TCGACAGACTTGAAGATGTA+AGG	0.499051	8:-66142397	MsaT044890.1:CDS
ATGCATTCTCATTCAACCTT+AGG	0.503609	8:+66143060	None:intergenic
GTTCCAAAGTTTAGCTTCAA+GGG	0.504136	8:-66142546	MsaT044890.1:CDS
GAAGGAACTGAAGTGGAGAC+AGG	0.505317	8:-66142993	MsaT044890.1:CDS
ATCGAATGAGATGCTGTTGA+GGG	0.506115	8:-66142639	MsaT044890.1:CDS
CCCGTGATTCGAACATGGCT+TGG	0.506282	8:+66142515	None:intergenic
TTGAAGAGGCTTGCAGAACA+TGG	0.509151	8:-66143464	MsaT044890.1:CDS
TTCTCCAATTCCCAAAACTC+TGG	0.510256	8:+66142024	None:intergenic
CAACAGCACTTACTTTGGGT+TGG	0.512066	8:-66141872	MsaT044890.1:CDS
TCCTCTATCTTGATCCTCAT+CGG	0.513315	8:+66142110	None:intergenic
CATCGAATGAGATGCTGTTG+AGG	0.514579	8:-66142640	MsaT044890.1:CDS
AGTTTGGCACAATTTGGAGC+TGG	0.514639	8:-66143350	MsaT044890.1:CDS
GTCTGAGCAGTTGTTGCTAA+AGG	0.515399	8:+66143536	None:intergenic
GGTGCAGAAATCACTTCAGC+AGG	0.516593	8:+66143224	None:intergenic
GCAGCAGCCGTTGAGGCCGG+TGG	0.517483	8:-66143636	MsaT044890.1:CDS
GCATGGAGGGTTCAGATGCA+TGG	0.518052	8:-66141988	MsaT044890.1:CDS
CATGATGATGTTGATGTTGG+TGG	0.518083	8:+66143132	None:intergenic
ACTTGGGAATTGGTGTAACC+TGG	0.522388	8:+66142675	None:intergenic
AGGAGAAGAGGACGAAGAAG+AGG	0.523774	8:-66142973	MsaT044890.1:CDS
TTTGAATTGTTCTGGAGATG+AGG	0.524360	8:-66143264	MsaT044890.1:CDS
CAGTGTTCACAAGCAGGAGC+AGG	0.525223	8:-66141901	MsaT044890.1:CDS
TTGCTTGCGAAGGAAGGGAC+AGG	0.525446	8:-66142058	MsaT044890.1:CDS
CAACATCATCATGTCAATGA+AGG	0.526407	8:-66143122	MsaT044890.1:CDS
ATTTACCTTGTAGCTTCCCT+CGG	0.526979	8:-66143679	MsaT044890.1:CDS
AGGGTGGGAGGAGTTTGAAG+AGG	0.527118	8:-66143478	MsaT044890.1:CDS
GACTACTGGATGAAAATCAA+AGG	0.528709	8:+66142418	None:intergenic
CAGCAGCCGTTGAGGCCGGT+GGG	0.533021	8:-66143635	MsaT044890.1:CDS
GTTACAGCAGATTCAAGCCA+AGG	0.534387	8:+66143182	None:intergenic
ACATTGTTGCTTGCGAAGGA+AGG	0.535871	8:-66142064	MsaT044890.1:CDS
CTGACTCTCTGATGAACCAT+TGG	0.536463	8:+66143091	None:intergenic
AGGAACATTGTTGCTTGCGA+AGG	0.538101	8:-66142068	MsaT044890.1:CDS
TCCAAGCCATGTTCGAATCA+CGG	0.544215	8:-66142516	MsaT044890.1:CDS
CATCAACACAACCAGTAAGC+AGG	0.548431	8:+66142925	None:intergenic
GTGATTCGAACATGGCTTGG+AGG	0.550236	8:+66142518	None:intergenic
TAATTACAGTGTTCACAAGC+AGG	0.552347	8:-66141907	MsaT044890.1:CDS
TCACTCCTTAATCTGTGAGT+TGG	0.553015	8:+66143764	None:intergenic
AAAACCAGCAAGTCTGTCTC+CGG	0.554388	8:+66142460	None:intergenic
CATTGTTGCTTGCGAAGGAA+GGG	0.554638	8:-66142063	MsaT044890.1:CDS
ATTGTTCTGGAGATGAGGAA+AGG	0.554859	8:-66143259	MsaT044890.1:CDS
CATGGAGGGTTCAGATGCAT+GGG	0.555361	8:-66141987	MsaT044890.1:CDS
AGAAAGATGATAGTGGAGCA+TGG	0.555422	8:-66142005	MsaT044890.1:CDS
GGAACACAATGAGGCGAGGT+TGG	0.556078	8:-66142213	MsaT044890.1:CDS
GCTTTGGATGCTTCATGTGA+AGG	0.557282	8:-66142375	MsaT044890.1:CDS
GATAGAGGAGATGTTAGCTA+GGG	0.557387	8:-66142096	MsaT044890.1:CDS
AAAGGGAAGGTTCAATGCCT+CGG	0.557666	8:+66142436	None:intergenic
ATTGGGAGAGATCAAGGATG+AGG	0.561558	8:-66143513	MsaT044890.1:CDS
TCAGAACATGAAGAGGGTAA+TGG	0.563710	8:-66143038	MsaT044890.1:CDS
TCATCGGACTCTCCAGTGGA+AGG	0.563830	8:+66142126	None:intergenic
GCTAAACTTTGGAACAAGCT+AGG	0.565024	8:+66142554	None:intergenic
AAACCATAGACGATGTTGGC+TGG	0.565074	8:-66143812	None:intergenic
GAATTGGAGAAAGATGATAG+TGG	0.568946	8:-66142012	MsaT044890.1:CDS
TGACATGATGATGTTGATGT+TGG	0.569622	8:+66143129	None:intergenic
ACTACTGGATGAAAATCAAA+GGG	0.570042	8:+66142419	None:intergenic
CCACACCCGTGATTCGAACA+TGG	0.572154	8:+66142510	None:intergenic
TGAAGTGGAGACAGGAGAAG+AGG	0.572761	8:-66142985	MsaT044890.1:CDS
CGCGAAGAACCGTCTTTCCG+AGG	0.572785	8:+66143662	None:intergenic
TGTAGCTTCCCTCGGAAAGA+CGG	0.573619	8:-66143671	MsaT044890.1:CDS
AGATGATAGTGGAGCATGGA+GGG	0.575789	8:-66142001	MsaT044890.1:CDS
GGCAATGAATGAATTCAAGC+TGG	0.576427	8:+66141774	None:intergenic
TCTTTCCGAGGGAAGCTACA+AGG	0.578020	8:+66143674	None:intergenic
AAGACACTTCATGATGGTAG+TGG	0.578818	8:-66142311	MsaT044890.1:CDS
GATGTGAAATGAAGAAACTT+GGG	0.580703	8:+66142659	None:intergenic
TCAGCATGAAGGAACTGAAG+TGG	0.582854	8:-66143000	MsaT044890.1:CDS
TAGGATTGGTGCTACGAATG+AGG	0.584060	8:+66142264	None:intergenic
GATATCCCCACCGGCCTCAA+CGG	0.585808	8:+66143629	None:intergenic
ATCCTCATCGGACTCTCCAG+TGG	0.587086	8:+66142122	None:intergenic
ATGCATGGGAGTCACTGATA+GGG	0.590517	8:-66141973	MsaT044890.1:CDS
AGCCAAGGATTGTTAGACAA+AGG	0.592331	8:+66143197	None:intergenic
AGCAGCCGTTGAGGCCGGTG+GGG	0.594224	8:-66143634	MsaT044890.1:CDS
TGTTCGAATCACGGGTGTGG+GGG	0.596570	8:-66142507	MsaT044890.1:CDS
ATGTTCGAATCACGGGTGTG+GGG	0.598829	8:-66142508	MsaT044890.1:CDS
GCAAGAAGCGGAACACAATG+AGG	0.609256	8:-66142222	MsaT044890.1:CDS
CTGGATGAAAATCAAAGGGA+AGG	0.609858	8:+66142423	None:intergenic
CGCGTTCGCAGCAGCCGTTG+AGG	0.611606	8:-66143643	MsaT044890.1:CDS
GGGAATCCTTATCAGCATGA+AGG	0.614596	8:-66143011	MsaT044890.1:CDS
TTGCGAAGGAAGGGACAGGT+TGG	0.618359	8:-66142054	MsaT044890.1:CDS
GAATGCATCAGAACATGAAG+AGG	0.618408	8:-66143045	MsaT044890.1:CDS
AATGAAGGTTCAGTCTCCAA+TGG	0.621452	8:-66143107	MsaT044890.1:CDS
ACACTTCATGATGGTAGTGG+AGG	0.624635	8:-66142308	MsaT044890.1:CDS
CAAGAAGATACTGTGTAGAG+AGG	0.631657	8:+66141840	None:intergenic
ATGAAACTGTCACTGCATTG+AGG	0.631836	8:-66142706	MsaT044890.1:CDS
GCGAAGAACCGTCTTTCCGA+GGG	0.631984	8:+66143663	None:intergenic
AAGGAAGGGACAGGTTGGAG+AGG	0.635039	8:-66142049	MsaT044890.1:CDS
TTCTCCACTGATATCCCCAC+CGG	0.635563	8:+66143620	None:intergenic
ATCGACTTCGACATAAAGCA+AGG	0.635920	8:-66142587	MsaT044890.1:CDS
AAGATGATAGTGGAGCATGG+AGG	0.636506	8:-66142002	MsaT044890.1:CDS
GTTCACAAGCAGGAGCAGGA+AGG	0.645596	8:-66141897	MsaT044890.1:CDS
AATGCATCAGAACATGAAGA+GGG	0.650132	8:-66143044	MsaT044890.1:CDS
CTTCACAAGACACTTCATGA+TGG	0.664343	8:-66142317	MsaT044890.1:CDS
TGAGCAGTTGTTGCTAAAGG+CGG	0.665701	8:+66143539	None:intergenic
GGATTGTTAGACAAAGGCAA+AGG	0.667360	8:+66143203	None:intergenic
ATGAAGAGGGTAATGGTTCG+GGG	0.669794	8:-66143031	MsaT044890.1:CDS
TGTTATGGCAGAGCAAGAAG+CGG	0.671377	8:-66142234	MsaT044890.1:CDS
ACATAAAGCAAGGACTACAA+TGG	0.673543	8:-66142577	MsaT044890.1:CDS
AAGCGGAACACAATGAGGCG+AGG	0.682806	8:-66142217	MsaT044890.1:CDS
GAGGCCGGTGGGGATATCAG+TGG	0.691692	8:-66143624	MsaT044890.1:CDS
TTCCACTGGAGAGTCCGATG+AGG	0.697132	8:-66142124	MsaT044890.1:CDS
TTTGATTCGATTGATCACAG+TGG	0.727954	8:-66142149	MsaT044890.1:CDS
GACGAAGAAGAGGAGCATCG+CGG	0.735685	8:-66142963	MsaT044890.1:CDS
TGTGAAGGAACATGAAACTG+TGG	0.739109	8:-66142360	MsaT044890.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	66141788	66143822	66141788	ID=MsaG044890
Chr8	mRNA	66141788	66143822	66141788	ID=MsaT044890.1;Parent=MsaG044890
Chr8	exon	66141788	66142766	66141788	ID=MsaT044890.1.exon2;Parent=MsaT044890.1
Chr8	CDS	66141788	66142766	66141788	ID=cds.MsaT044890.1;Parent=MsaT044890.1
Chr8	exon	66142918	66143822	66142918	ID=MsaT044890.1.exon1;Parent=MsaT044890.1
Chr8	CDS	66142918	66143822	66142918	ID=cds.MsaT044890.1;Parent=MsaT044890.1

Gene Sequence

>MsaT044890.1

ATGTTGGCTGGTTGTTCTAGCTCGACATTGTTGTCACCAACTCACAGATTAAGGAGTGAAGCATCAGCACAGTTTCAAGCTTGTCATTTTCAGCTACCATCAATGAGCACACAGAGATTGGATTTACCTTGTAGCTTCCCTCGGAAAGACGGTTCTTCGCGTTCGCAGCAGCCGTTGAGGCCGGTGGGGATATCAGTGGAGAAGCCTGAAGTCAAGAACAACAGCAGTAGCACTTGTTCTCTGCAGCAGAACATTCGGTTACCGCCTTTAGCAACAACTGCTCAGACATTGGGAGAGATCAAGGATGAGGAGTTTTGGGAGAAGGGTGGGAGGAGTTTGAAGAGGCTTGCAGAACATGGATCTTTGGATGAGTCTTTTGCAAATAGAGCTAAGAGGAAAAAGGGTAATGGTGGTGATGAGAATTTTGATGAATTTGAAAGGGAAAGTTTCAGTTTGGCACAATTTGGAGCTGGAAACTTTTGGTCTGTTCACAGTTTTGGAGGGTTTAATAGTAATAGCTCTTCGATTCCTTTCTCTTTGAATTGTTCTGGAGATGAGGAAAGGGTTTGTTTTGCTCCTGCTGAAGTGATTTCTGCACCTTTGCCTTTGTCTAACAATCCTTGGCTTGAATCTGCTGTAACAAAGATCACCAATTTTGGTGAGGGTAGCCACCAACATCAACATCATCATGTCAATGAAGGTTCAGTCTCCAATGGTTCATCAGAGAGTCAGAGTTTGAGCCTAAGGTTGAATGAGAATGCATCAGAACATGAAGAGGGTAATGGTTCGGGGAATCCTTATCAGCATGAAGGAACTGAAGTGGAGACAGGAGAAGAGGACGAAGAAGAGGAGCATCGCGGTTTTGAGCTTTTGAGCCTGCTTACTGGTTGTGTTGATGCTATTGGCGCTACTTCTCGTGATTTTGACAGGGTGGTGGATGATGATGAAACTGTCACTGCATTGAGGCTTCTAAACCAGGTTACACCAATTCCCAAGTTTCTTCATTTCACATCGAATGAGATGCTGTTGAGGGCTTTTGAAGGGAAAGAAAGGGTTCACATTATCGACTTCGACATAAAGCAAGGACTACAATGGCCTAGCTTGTTCCAAAGTTTAGCTTCAAGGGCCAATCCTCCAAGCCATGTTCGAATCACGGGTGTGGGGGAATCGAAGCAAGAGCTGAATGAAACCGGAGACAGACTTGCTGGTTTTGCCGAGGCATTGAACCTTCCCTTTGATTTTCATCCAGTAGTCGACAGACTTGAAGATGTAAGGCTTTGGATGCTTCATGTGAAGGAACATGAAACTGTGGCTGTGAACTGTGTTTTGCAGCTTCACAAGACACTTCATGATGGTAGTGGAGGAGCATTGAGAGACTTTTTGGGCCTCATTCGTAGCACCAATCCTACAATTGTTGTTATGGCAGAGCAAGAAGCGGAACACAATGAGGCGAGGTTGGACTCAAGAGTCTGCAATTCATTGAAATATTACTCAGCGCTGTTTGATTCGATTGATCACAGTGGCCTTCCACTGGAGAGTCCGATGAGGATCAAGATAGAGGAGATGTTAGCTAGGGAGATAAGGAACATTGTTGCTTGCGAAGGAAGGGACAGGTTGGAGAGGCACCAGAGTTTTGGGAATTGGAGAAAGATGATAGTGGAGCATGGAGGGTTCAGATGCATGGGAGTCACTGATAGGGAACTGATTCAGAGCCAGTTTCTGTTGAAGATGTATTCTTGTGATAATTACAGTGTTCACAAGCAGGAGCAGGAAGGAGCAACAGCACTTACTTTGGGTTGGCTTGATCAACCTCTCTACACAGTATCTTCTTGGACACCGGTTGACGTTGCTGGAAGCTCATCCTCTTTTTCTCAGCCAGCTTGA

Protein sequence

>MsaT044890.1

MLAGCSSSTLLSPTHRLRSEASAQFQACHFQLPSMSTQRLDLPCSFPRKDGSSRSQQPLRPVGISVEKPEVKNNSSSTCSLQQNIRLPPLATTAQTLGEIKDEEFWEKGGRSLKRLAEHGSLDESFANRAKRKKGNGGDENFDEFERESFSLAQFGAGNFWSVHSFGGFNSNSSSIPFSLNCSGDEERVCFAPAEVISAPLPLSNNPWLESAVTKITNFGEGSHQHQHHHVNEGSVSNGSSESQSLSLRLNENASEHEEGNGSGNPYQHEGTEVETGEEDEEEEHRGFELLSLLTGCVDAIGATSRDFDRVVDDDETVTALRLLNQVTPIPKFLHFTSNEMLLRAFEGKERVHIIDFDIKQGLQWPSLFQSLASRANPPSHVRITGVGESKQELNETGDRLAGFAEALNLPFDFHPVVDRLEDVRLWMLHVKEHETVAVNCVLQLHKTLHDGSGGALRDFLGLIRSTNPTIVVMAEQEAEHNEARLDSRVCNSLKYYSALFDSIDHSGLPLESPMRIKIEEMLAREIRNIVACEGRDRLERHQSFGNWRKMIVEHGGFRCMGVTDRELIQSQFLLKMYSCDNYSVHKQEQEGATALTLGWLDQPLYTVSSWTPVDVAGSSSSFSQPA*