AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG044948

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044948 MtrunA17_Chr4g0058071 97.796 363 7 1 1 363 1 362 0.0 744
MsaG044948 MtrunA17_Chr3g0116501 58.621 203 81 2 87 289 1 200 1.57e-86 260
MsaG044948 MtrunA17_Chr5g0401281 45.973 298 154 7 18 313 68 360 1.56e-84 261
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG044948 AT3G48360.1 61.515 330 114 6 15 332 32 360 2.03e-133 385
MsaG044948 AT5G63160.1 62.540 315 108 5 14 319 22 335 2.64e-130 377
MsaG044948 AT3G48360.2 63.636 286 92 5 15 289 32 316 3.39e-120 351
MsaG044948 AT5G63160.2 63.732 284 94 4 14 289 22 304 3.16e-119 347
MsaG044948 AT5G63160.3 64.634 246 79 4 81 319 6 250 8.10e-104 307
MsaG044948 AT3G48360.3 62.745 255 85 4 87 332 1 254 1.59e-103 305
MsaG044948 AT5G67480.1 45.364 302 159 6 18 317 61 358 7.24e-83 257
MsaG044948 AT5G67480.3 45.364 302 159 6 18 317 61 358 7.24e-83 257
MsaG044948 AT5G67480.2 45.364 302 159 6 18 317 72 369 9.61e-83 257
MsaG044948 AT4G37610.1 42.904 303 164 8 18 317 56 352 6.86e-76 239
MsaG044948 AT1G05690.1 41.528 301 170 5 18 317 55 350 1.82e-74 235
MsaG044948 AT1G55760.1 33.835 133 79 4 18 147 165 291 6.35e-13 69.3
MsaG044948 AT3G56230.1 24.845 161 120 1 25 184 120 280 2.11e-12 67.4

Find 97 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAAGGAACGCCGTGAATT+TGG 0.203367 8:-66804079 None:intergenic
CTTGCAATGTTCCTCTTTGC+AGG 0.245278 8:+66804993 MsaT044948.1:CDS
CCTTGCTAGTAGTTTCCATT+TGG 0.271352 8:-66807197 None:intergenic
GTGAATTTGGATTATTCTTT+CGG 0.293435 8:-66804066 None:intergenic
ATGAATAAAGACATCATATT+CGG 0.296060 8:-66803844 None:intergenic
TTCTGAGGCGATGGAGTGTT+TGG 0.329039 8:+66804748 MsaT044948.1:CDS
ACTTGATATCCTCCGCTTCA+TGG 0.330110 8:+66804497 MsaT044948.1:CDS
GACCTCCATTGGTGTGGTTT+GGG 0.335478 8:-66803814 None:intergenic
AGAGGGTGAAGGGAGGGTGC+TGG 0.344939 8:+66804906 MsaT044948.1:CDS
CTTGCTAGTAGTTTCCATTT+GGG 0.348881 8:-66807196 None:intergenic
AATCGTGGGATCAGATGTTT+TGG 0.358065 8:+66807301 MsaT044948.1:CDS
CGACCTCCATTGGTGTGGTT+TGG 0.364602 8:-66803815 None:intergenic
TGCAAGAATCATCAGTTTGT+TGG 0.365276 8:-66804976 None:intergenic
TCTGATCCCACGATTGTCTA+TGG 0.399851 8:-66807293 None:intergenic
TCAATAATGTTCTCGAAAAC+AGG 0.405232 8:-66804020 None:intergenic
CAGTTGCACGGAGGATGAAA+TGG 0.408781 8:+66804227 MsaT044948.1:intron
ACCAGGATGCTTGTGTGGGC+TGG 0.439380 8:-66803888 None:intergenic
TTCAAGCACAGGATCATGTT+TGG 0.441776 8:-66804475 None:intergenic
CGCCAGGCTCTGCGATGCAC+CGG 0.448288 8:+66804374 MsaT044948.1:CDS
CAGACAATTTCAATTGAGAA+TGG 0.449145 8:+66807150 MsaT044948.1:intron
TGAATAAAGACATCATATTC+GGG 0.450560 8:-66803843 None:intergenic
TTATTAACCTCAACATGGTA+TGG 0.459852 8:-66804806 None:intergenic
TTGAACCGCGTTGAAGTGAT+TGG 0.469408 8:-66804427 None:intergenic
TGAGAATGGAGCAAGAGAAA+AGG 0.470643 8:+66807164 MsaT044948.1:CDS
GTCTTTATTCATACTTCTGA+TGG 0.471148 8:+66803855 MsaT044948.1:CDS
GATGAAAGCGGTTACGGCGG+TGG 0.476910 8:-66804099 None:intergenic
GTAGCCGATGAAAGCGGTTA+CGG 0.477754 8:-66804105 None:intergenic
ATCCGGTGCATCGCAGAGCC+TGG 0.478068 8:-66804376 None:intergenic
AGCAAATTCTCCACCTGTCA+AGG 0.481574 8:+66804845 MsaT044948.1:CDS
AAAGGAAAGATGATCCCAAA+TGG 0.490170 8:+66807182 MsaT044948.1:CDS
CAGAAGCTGAAGCCCTTGAC+AGG 0.502805 8:-66804858 None:intergenic
AACTAACCAGGATGCTTGTG+TGG 0.507204 8:-66803893 None:intergenic
CATCGCAGAGCCTGGCGAGT+TGG 0.510849 8:-66804368 None:intergenic
TGCGGAACTTTCTGAGGCGA+TGG 0.528118 8:+66804739 MsaT044948.1:CDS
TCGGCTACCTCTACTCATCC+AGG 0.528548 8:+66804120 MsaT044948.1:CDS
TATAGAGGAGAGAGAAATCG+AGG 0.529791 8:+66804690 MsaT044948.1:intron
GAAAGAATAATCCAAATTCA+CGG 0.535992 8:+66804068 MsaT044948.1:CDS
TCCAGCCCACACAAGCATCC+TGG 0.542058 8:+66803887 MsaT044948.1:CDS
CTCAAACAAAGATGCATCAA+AGG 0.544591 8:+66804303 MsaT044948.1:CDS
TCGGGTAAGACGACCTCCAT+TGG 0.548298 8:-66803825 None:intergenic
GTGTTTGGAGCATATATGTA+CGG 0.548333 8:+66804763 MsaT044948.1:CDS
GAGGATGAAATGGACAAATA+CGG 0.550313 8:+66804237 MsaT044948.1:CDS
ACCGCCGTAACCGCTTTCAT+CGG 0.550954 8:+66804101 MsaT044948.1:CDS
ACACGTGAGATAGTGCCAAC+AGG 0.553618 8:-66804266 None:intergenic
GGGTGAAGGGAGGGTGCTGG+AGG 0.555902 8:+66804909 MsaT044948.1:CDS
ACATGCAAGAAGAGGGTGAA+GGG 0.559984 8:+66804896 MsaT044948.1:CDS
ATGGAAACTACTAGCAAGGA+AGG 0.562140 8:+66807201 MsaT044948.1:CDS
TGGATGTACTCCAACTCGCC+AGG 0.562979 8:+66804358 MsaT044948.1:CDS
CGCTTTCGTCCATGAAGCGG+AGG 0.563948 8:-66804506 None:intergenic
ATGCATCTTTGTTTGAGTTG+CGG 0.567744 8:-66804297 None:intergenic
TTTCGCTTTCGTCCATGAAG+CGG 0.570395 8:-66804509 None:intergenic
AAAGCGGTTACGGCGGTGGA+AGG 0.571422 8:-66804095 None:intergenic
GCATCAAAGGTCTTGTTCAA+CGG 0.574251 8:+66804316 MsaT044948.1:CDS
CAAATACGGAATCCACCTGT+TGG 0.576636 8:+66804251 MsaT044948.1:CDS
AAGGAAGGTTGCCTCAGCTA+AGG 0.578016 8:+66807216 MsaT044948.1:CDS
GCAAGAAGAGGGTGAAGGGA+GGG 0.583602 8:+66804900 MsaT044948.1:CDS
CCAAATGGAAACTACTAGCA+AGG 0.585999 8:+66807197 MsaT044948.1:CDS
TTGGAGCATATATGTACGGA+AGG 0.591498 8:+66804767 MsaT044948.1:CDS
AGGATATCAAGTTCAAGCAC+AGG 0.591612 8:-66804486 None:intergenic
GGAAGCATAGAGAGGAGCAA+GGG 0.591806 8:+66804711 MsaT044948.1:CDS
ATACAACGGATACGGAGATC+CGG 0.598941 8:-66804393 None:intergenic
GCAAATTCTCCACCTGTCAA+GGG 0.602836 8:+66804846 MsaT044948.1:CDS
GTTGTATGCGGAACTTTCTG+AGG 0.605270 8:+66804733 MsaT044948.1:CDS
GTAGCTTCATACAACGGATA+CGG 0.605346 8:-66804401 None:intergenic
GAATACCCAAACCACACCAA+TGG 0.607266 8:+66803809 MsaT044948.1:CDS
TCGGAGCTGCGTTGTTTACG+CGG 0.613669 8:-66804047 None:intergenic
AAGCTGAAGCCCTTGACAGG+TGG 0.613763 8:-66804855 None:intergenic
AGGAAGCATAGAGAGGAGCA+AGG 0.616562 8:+66804710 MsaT044948.1:CDS
TAAACAACTAACCTGCAAAG+AGG 0.620623 8:-66805004 None:intergenic
TGCAAGAAGAGGGTGAAGGG+AGG 0.622160 8:+66804899 MsaT044948.1:CDS
CAGGTGGAGAATTTGCTACA+CGG 0.624063 8:-66804839 None:intergenic
CGTTGGACCATACCATGTTG+AGG 0.625619 8:+66804799 MsaT044948.1:CDS
TACCCAAACCACACCAATGG+AGG 0.626720 8:+66803812 MsaT044948.1:CDS
TAAGACGACCTCCATTGGTG+TGG 0.627752 8:-66803820 None:intergenic
CTACAAACCTGGATGAGTAG+AGG 0.636623 8:-66804127 None:intergenic
ATATTGTAACAATAATGTCT+CGG 0.638704 8:+66807333 MsaT044948.1:CDS
GCTGGAGGTGCAAACGTATG+TGG 0.642359 8:+66804924 MsaT044948.1:CDS
TTTCTTTATTAACCTCAACA+TGG 0.646312 8:-66804811 None:intergenic
GAAATCGAGGAAGCATAGAG+AGG 0.652687 8:+66804703 MsaT044948.1:CDS
GCACAGGATCATGTTTGGTG+AGG 0.659496 8:-66804470 None:intergenic
GAGTGACCATAGACAATCGT+GGG 0.660262 8:+66807287 MsaT044948.1:CDS
GCCGATGAAAGCGGTTACGG+CGG 0.661788 8:-66804102 None:intergenic
ACGGAAGGGTGCACCGACGT+TGG 0.666831 8:+66804782 MsaT044948.1:CDS
CGTGAGATAGTGCCAACAGG+TGG 0.667055 8:-66804263 None:intergenic
GGAAAACATAACCTTAGCTG+AGG 0.673989 8:-66807227 None:intergenic
GTAGAGGTAGCCGATGAAAG+CGG 0.674697 8:-66804111 None:intergenic
AACATGCAAGAAGAGGGTGA+AGG 0.677915 8:+66804895 MsaT044948.1:CDS
TGGAGCATATATGTACGGAA+GGG 0.681205 8:+66804768 MsaT044948.1:CDS
AGAGGAGCAAGGGTTGTATG+CGG 0.686478 8:+66804721 MsaT044948.1:CDS
TGGTGAGTAGCTTCATACAA+CGG 0.689964 8:-66804407 None:intergenic
ACTAACCAGGATGCTTGTGT+GGG 0.692654 8:-66803892 None:intergenic
CTCGAAAACAGGAGATACCG+AGG 0.700797 8:-66804009 None:intergenic
AGAGTGACCATAGACAATCG+TGG 0.708473 8:+66807286 MsaT044948.1:CDS
ACGGTTAACATCAGAAAACG+TGG 0.721632 8:+66804335 MsaT044948.1:CDS
AACATGGTATGGTCCAACGT+CGG 0.721753 8:-66804795 None:intergenic
GTTAACATCAGAAAACGTGG+TGG 0.747454 8:+66804338 MsaT044948.1:CDS
ACTCACCAATCACTTCAACG+CGG 0.835800 8:+66804422 MsaT044948.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 66803801 66807378 66803801 ID=MsaG044948
Chr8 mRNA 66803801 66807378 66803801 ID=MsaT044948.1;Parent=MsaG044948
Chr8 exon 66803801 66803908 66803801 ID=MsaT044948.1.exon1;Parent=MsaT044948.1
Chr8 CDS 66803801 66803908 66803801 ID=cds.MsaT044948.1;Parent=MsaT044948.1
Chr8 exon 66804008 66804141 66804008 ID=MsaT044948.1.exon2;Parent=MsaT044948.1
Chr8 CDS 66804008 66804141 66804008 ID=cds.MsaT044948.1;Parent=MsaT044948.1
Chr8 exon 66804230 66804533 66804230 ID=MsaT044948.1.exon3;Parent=MsaT044948.1
Chr8 CDS 66804230 66804533 66804230 ID=cds.MsaT044948.1;Parent=MsaT044948.1
Chr8 exon 66804695 66805014 66804695 ID=MsaT044948.1.exon4;Parent=MsaT044948.1
Chr8 CDS 66804695 66805014 66804695 ID=cds.MsaT044948.1;Parent=MsaT044948.1
Chr8 exon 66807153 66807378 66807153 ID=MsaT044948.1.exon5;Parent=MsaT044948.1
Chr8 CDS 66807153 66807378 66807153 ID=cds.MsaT044948.1;Parent=MsaT044948.1
Gene Sequence

>MsaT044948.1

ATGGAAATGAATACCCAAACCACACCAATGGAGGTCGTCTTACCCGAATATGATGTCTTTATTCATACTTCTGATGGCACACGCATTCCAGCCCACACAAGCATCCTGGCCTCGGTATCTCCTGTTTTCGAGAACATTATTGATCAACCGCGTAAACAACGCAGCTCCGAAAGAATAATCCAAATTCACGGCGTTCCTTCCACCGCCGTAACCGCTTTCATCGGCTACCTCTACTCATCCAGTTGCACGGAGGATGAAATGGACAAATACGGAATCCACCTGTTGGCACTATCTCACGTGTACAATGTGCCGCAACTCAAACAAAGATGCATCAAAGGTCTTGTTCAACGGTTAACATCAGAAAACGTGGTGGATGTACTCCAACTCGCCAGGCTCTGCGATGCACCGGATCTCCGTATCCGTTGTATGAAGCTACTCACCAATCACTTCAACGCGGTTCAAAAAACTGAAGGGTGGAAGTTCCTCACCAAACATGATCCTGTGCTTGAACTTGATATCCTCCGCTTCATGGACGAAAGCGAAACAAGGAGAGAGAAATCGAGGAAGCATAGAGAGGAGCAAGGGTTGTATGCGGAACTTTCTGAGGCGATGGAGTGTTTGGAGCATATATGTACGGAAGGGTGCACCGACGTTGGACCATACCATGTTGAGGTTAATAAAGAAAAGAAACCGTGTAGCAAATTCTCCACCTGTCAAGGGCTTCAGCTTCTGATTCGTCATTTTGCAACATGCAAGAAGAGGGTGAAGGGAGGGTGCTGGAGGTGCAAACGTATGTGGCAGCTGTTTAGATTGCATTCGTATGTTTGCCAACAAACTGATGATTCTTGCAATGTTCCTCTTTGCAGACAATTTCAATTGAGAATGGAGCAAGAGAAAAGGAAAGATGATCCCAAATGGAAACTACTAGCAAGGAAGGTTGCCTCAGCTAAGGTTATGTTTTCCTTGTCTCTCCCTAAGAGAAAAAGAGATGAAGAAATGAGAGTGACCATAGACAATCGTGGGATCAGATGTTTTGGTTACAATGAATATTGTAACAATAATGTCTCGGACAATCGATGTGTTTCATGCTGA
Protein sequence

>MsaT044948.1

MEMNTQTTPMEVVLPEYDVFIHTSDGTRIPAHTSILASVSPVFENIIDQPRKQRSSERIIQIHGVPSTAVTAFIGYLYSSSCTEDEMDKYGIHLLALSHVYNVPQLKQRCIKGLVQRLTSENVVDVLQLARLCDAPDLRIRCMKLLTNHFNAVQKTEGWKFLTKHDPVLELDILRFMDESETRREKSRKHREEQGLYAELSEAMECLEHICTEGCTDVGPYHVEVNKEKKPCSKFSTCQGLQLLIRHFATCKKRVKGGCWRCKRMWQLFRLHSYVCQQTDDSCNVPLCRQFQLRMEQEKRKDDPKWKLLARKVASAKVMFSLSLPKRKRDEEMRVTIDNRGIRCFGYNEYCNNNVSDNRCVSC*