AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045075

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045075 MtrunA17_Chr4g0059371 96.133 181 7 0 1 181 1 181 4.97e-131 364
MsaG045075 MtrunA17_Chr1g0152431 67.429 175 56 1 3 176 2 176 2.34e-89 259
MsaG045075 MtrunA17_Chr3g0113051 54.945 182 77 3 3 181 2 181 3.72e-69 208
MsaG045075 MtrunA17_Chr8g0386501 50.838 179 81 2 3 181 2 173 4.86e-62 191
MsaG045075 MtrunA17_Chr8g0386501 53.521 71 33 0 2 72 93 163 5.87e-20 83.2
MsaG045075 MtrunA17_Chr8g0349391 50.279 179 76 2 3 177 2 171 4.53e-60 186
MsaG045075 MtrunA17_Chr7g0247111 49.133 173 81 2 3 173 2 169 4.97e-58 181
MsaG045075 MtrunA17_Chr7g0247111 43.038 79 45 0 4 82 99 177 1.80e-15 71.2
MsaG045075 MtrunA17_Chr7g0247111 43.056 72 41 0 103 174 3 74 3.52e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045075 AT1G10200.1 69.143 175 53 1 3 176 2 176 1.40e-90 263
MsaG045075 AT3G55770.3 56.111 180 76 2 3 181 2 179 1.66e-70 212
MsaG045075 AT3G55770.5 56.111 180 76 2 3 181 2 179 1.66e-70 212
MsaG045075 AT3G55770.2 56.111 180 76 2 3 181 2 179 1.66e-70 212
MsaG045075 AT3G55770.1 56.111 180 76 2 3 181 2 179 1.66e-70 212
MsaG045075 AT2G39900.1 53.073 179 84 0 3 181 2 180 3.42e-69 209
MsaG045075 AT3G61230.1 53.179 173 78 1 1 173 1 170 6.19e-67 204
MsaG045075 AT3G61230.1 40.659 91 54 0 4 94 100 190 1.20e-17 77.4
MsaG045075 AT3G61230.1 44.444 72 40 0 103 174 4 75 6.48e-16 72.8
MsaG045075 AT2G45800.1 53.488 172 75 2 3 173 2 169 4.83e-63 194
MsaG045075 AT2G45800.1 46.512 86 46 0 4 89 99 184 9.23e-18 78.2
MsaG045075 AT2G45800.1 45.833 72 39 0 103 174 3 74 1.42e-16 74.7
MsaG045075 AT1G01780.1 52.047 171 77 2 3 173 2 167 5.44e-63 193
MsaG045075 AT1G01780.1 47.222 72 38 0 103 174 3 74 3.84e-17 75.9
MsaG045075 AT3G55770.7 47.196 214 76 3 3 181 2 213 7.38e-63 194
MsaG045075 AT1G10200.2 65.909 132 44 1 46 176 3 134 1.42e-62 190
MsaG045075 AT1G10200.2 47.222 72 38 0 4 75 61 132 2.95e-16 72.4
MsaG045075 AT3G55770.4 58.197 122 48 2 3 123 2 121 1.30e-43 142
MsaG045075 AT3G55770.4 47.222 72 38 0 103 174 3 74 3.35e-18 77.0
MsaG045075 AT2G45800.2 50.000 140 64 3 35 173 4 138 9.73e-43 141
MsaG045075 AT2G45800.2 46.512 86 46 0 4 89 68 153 3.39e-18 78.6
MsaG045075 AT3G55770.6 55.738 122 51 2 3 123 2 121 1.42e-40 134
MsaG045075 AT3G55770.6 47.222 72 38 0 103 174 3 74 2.03e-18 77.4

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAAGGTTGAACAAGTCTA+AGG 0.271390 8:-68136662 None:intergenic
CTTGTTCAACCTTATCAAAT+TGG 0.314326 8:+68136669 None:intergenic
CTTTAAGATGACTGGCAGTT+TGG 0.324339 8:-68138021 MsaT045075.1:CDS
TTTGAGATGGCATCATTTGC+AGG 0.338265 8:-68139858 None:intergenic
CATGTGAAAAGAAAGTTTAC+TGG 0.388914 8:-68139815 MsaT045075.1:CDS
TTGAAACCTTGTTATATGCT+TGG 0.394784 8:+68137735 None:intergenic
TATGGTGATGTCTACAATAA+AGG 0.395154 8:+68136723 None:intergenic
TCATGTGCCACATAGTTTGA+TGG 0.445617 8:+68136752 None:intergenic
TCTGTTTGAAGATCTGAGTA+TGG 0.453506 8:+68136705 None:intergenic
TAATTTCAGCCAATTTGATA+AGG 0.454495 8:-68136678 MsaT045075.1:CDS
CTTACCATAAGACTTGCTTC+AGG 0.455370 8:-68136802 MsaT045075.1:CDS
AGTTGAAAGATCAACTGATC+AGG 0.484362 8:-68137846 MsaT045075.1:intron
GATCAACTCTTTAAGATGAC+TGG 0.501872 8:-68138029 MsaT045075.1:CDS
AACAGTGTACCCAATTGAAA+AGG 0.511293 8:-68137669 MsaT045075.1:intron
TTAAAGAGTTGATCAAAATG+AGG 0.517865 8:+68138038 None:intergenic
TGCACCTGAAGCAAGTCTTA+TGG 0.519828 8:+68136798 None:intergenic
TACTCAGATCTTCAAACAGA+AGG 0.531322 8:-68136702 MsaT045075.1:CDS
CTTCAGATGCCACCATTGCA+AGG 0.542591 8:-68139748 MsaT045075.1:CDS
TGAGTGTGCCCTTGCAATGG+TGG 0.554362 8:+68139739 None:intergenic
ACTTGCTTCAGGTGCACACA+TGG 0.555007 8:-68136791 MsaT045075.1:CDS
TTCAGATGCCACCATTGCAA+GGG 0.577839 8:-68139747 MsaT045075.1:CDS
ATAAGGTTGAACAAGTCTAA+GGG 0.583079 8:-68136661 None:intergenic
CCTTGAGTGTGCCCTTGCAA+TGG 0.588637 8:+68139736 None:intergenic
ATGTGAAAAGAAAGTTTACT+GGG 0.603879 8:-68139814 MsaT045075.1:CDS
ACTCAGATCTTCAAACAGAA+GGG 0.607367 8:-68136701 MsaT045075.1:CDS
AATCAGTCCATCAAACTATG+TGG 0.634185 8:-68136759 MsaT045075.1:CDS
CCATTGCAAGGGCACACTCA+AGG 0.651170 8:-68139736 MsaT045075.1:intron
TGCTTCAGGTGCACACATGG+AGG 0.682276 8:-68136788 MsaT045075.1:CDS
ACAGGTCCAAGCATATAACA+AGG 0.699921 8:-68137741 MsaT045075.1:intron
TGAAAAGAAAGTTTACTGGG+TGG 0.703991 8:-68139811 MsaT045075.1:CDS
GGCATCTGAAGCAAGACTTG+TGG 0.735982 8:+68139760 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 68136664 68139874 68136664 ID=MsaG045075
Chr8 mRNA 68136664 68139874 68136664 ID=MsaT045075.1;Parent=MsaG045075
Chr8 exon 68136664 68136843 68136664 ID=MsaT045075.1.exon5;Parent=MsaT045075.1
Chr8 CDS 68136664 68136843 68136664 ID=cds.MsaT045075.1;Parent=MsaT045075.1
Chr8 exon 68137670 68137759 68137670 ID=MsaT045075.1.exon4;Parent=MsaT045075.1
Chr8 CDS 68137670 68137759 68137670 ID=cds.MsaT045075.1;Parent=MsaT045075.1
Chr8 exon 68137847 68137887 68137847 ID=MsaT045075.1.exon3;Parent=MsaT045075.1
Chr8 CDS 68137847 68137887 68137847 ID=cds.MsaT045075.1;Parent=MsaT045075.1
Chr8 exon 68138006 68138102 68138006 ID=MsaT045075.1.exon2;Parent=MsaT045075.1
Chr8 CDS 68138006 68138102 68138006 ID=cds.MsaT045075.1;Parent=MsaT045075.1
Chr8 exon 68139737 68139874 68139737 ID=MsaT045075.1.exon1;Parent=MsaT045075.1
Chr8 CDS 68139737 68139874 68139737 ID=cds.MsaT045075.1;Parent=MsaT045075.1
Gene Sequence

>MsaT045075.1

ATGGCATCATTTGCAGGCACTACACAGAAATGTAATACATGTGAAAAGAAAGTTTACTGGGTGGAACAGCTCACTGCTGATAACAAAGTGTACCACAAGTCTTGCTTCAGATGCCACCATTGCAAGGGCACACTCAAGCTGAGTAACTATTGTTCTTTTGAGGGTGTTCTATACTGTAAGCCTCATTTTGATCAACTCTTTAAGATGACTGGCAGTTTGGACAAAAGTTTTGAAGGTATTGCAAGAATTTATAGAGTTGAAAGATCAACTGATCAGGTCCAAGCATATAACAAGGTTTCAAGATTTTTTGCTGGAACTCAAGAAAAATGTGTTGGTTGCAAGAAAACAGTGTACCCAATTGAAAAGGTAGCTGTTGATGGAGAATCTTACCATAAGACTTGCTTCAGGTGCACACATGGAGGTTGCATAATCAGTCCATCAAACTATGTGGCACATGAACATCGCCTTTATTGTAGACATCACCATACTCAGATCTTCAAACAGAAGGGTAATTTCAGCCAATTTGATAAGGTTGAACAAGTCTAA
Protein sequence

>MsaT045075.1

MASFAGTTQKCNTCEKKVYWVEQLTADNKVYHKSCFRCHHCKGTLKLSNYCSFEGVLYCKPHFDQLFKMTGSLDKSFEGIARIYRVERSTDQVQAYNKVSRFFAGTQEKCVGCKKTVYPIEKVAVDGESYHKTCFRCTHGGCIISPSNYVAHEHRLYCRHHHTQIFKQKGNFSQFDKVEQV*