AlfalfaGEDB | MsaG045088

M. sativa ssp. caerulea / MsaG045088

KEGG: K09391
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006355 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032875 GO:0032876 GO:0034641 GO:0034645 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045786 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051252 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1903506 GO:2000104 GO:2000112 GO:2000113 GO:2001141
PFAM: PF02319.23

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045088	AES91128.2	98.324	358	5	1	1	357	1	358	0.0	727

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045088	sp\|Q8LSZ4\|E2FE_ARATH	54.469	358	133	11	12	355	29	370	2.02e-109	328

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045088	tr\|G7JNS6\|G7JNS6_MEDTR	98.324	358	5	1	1	357	1	358	0.0	727

TFs/TRs:

Gene ID	Type	Classification
MsaG045088	TF	E2F-DP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG006940	MsaG045088	0.800106	2.813640e-48	3.813283e-46
MsaG011077	MsaG045088	0.810353	2.056535e-50	3.542453e-48
MsaG045087	MsaG045088	0.977575	2.227390e-143	4.361739e-137
MsaG013574	MsaG045088	0.809514	3.111831e-50	5.252302e-48
MsaG028998	MsaG045088	0.804651	3.291516e-49	4.949857e-47
MsaG036173	MsaG045088	0.809494	3.142012e-50	5.300806e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045088	MtrunA17_Chr4g0059551	98.039	357	6	1	1	356	1	357	0.0	724
MsaG045088	MtrunA17_Chr4g0025881	46.269	67	36	0	17	83	138	204	3.53e-12	67.4
MsaG045088	MtrunA17_Chr3g0142581	46.377	69	37	0	14	82	99	167	1.12e-11	65.5
MsaG045088	MtrunA17_Chr1g0177351	44.776	67	37	0	17	83	142	208	2.67e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG045088	AT3G48160.1	55.000	360	130	12	12	356	29	371	2.16e-112	332
MsaG045088	AT3G48160.2	54.469	358	133	11	12	355	29	370	2.06e-110	328
MsaG045088	AT3G01330.1	47.813	343	150	5	13	355	17	330	1.38e-95	288
MsaG045088	AT5G14960.1	41.761	352	169	9	13	354	9	334	8.10e-80	248
MsaG045088	AT5G14960.2	37.915	211	114	6	154	354	38	241	2.86e-34	127
MsaG045088	AT5G22220.2	44.776	67	37	0	17	83	129	195	4.67e-11	64.3
MsaG045088	AT5G22220.3	44.776	67	37	0	17	83	129	195	4.99e-11	64.3

Find 84 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATCTGGGAAAAGTTGAATT+AGG	0.171110	8:+68316222	None:intergenic
AAAGACGATGTTCGTTTAAT+TGG	0.211016	8:-68316968	MsaT045088.1:CDS
CACCCTGACTTCCAGTGGTT+TGG	0.211662	8:+68316289	None:intergenic
GGCAATTCCTGTTGCTTTAC+AGG	0.216414	8:-68316609	MsaT045088.1:CDS
TTCAGGTGGCTAGGCTTAAA+AGG	0.224076	8:-68314650	MsaT045088.1:CDS
GTAATAATGAATACCTAATT+TGG	0.256592	8:+68316928	None:intergenic
ATTCAAAACAAAACGATTCT+AGG	0.280464	8:-68314598	MsaT045088.1:CDS
TTTCCAGGTATACTGATTCT+TGG	0.296038	8:+68316640	None:intergenic
AGTATATTACCTTCTCAATA+AGG	0.302257	8:+68314832	None:intergenic
GACGACGCTGCCGCCAAATT+AGG	0.309438	8:-68316941	MsaT045088.1:intron
ACCCTGACTTCCAGTGGTTT+GGG	0.311184	8:+68316290	None:intergenic
ATCTGGGAAAAGTTGAATTA+GGG	0.323897	8:+68316223	None:intergenic
TACCTTCTCAATAAGGTTCA+TGG	0.335894	8:+68314839	None:intergenic
AATTGCCGCAAAACCTTTCC+AGG	0.340573	8:+68316625	None:intergenic
TGTTGCTTTACAGGAGCTTA+AGG	0.369256	8:-68316600	MsaT045088.1:intron
ATTCTCAGAGCTTCCAGCTT+TGG	0.369624	8:+68314382	None:intergenic
ATCCCAAACCACTGGAAGTC+AGG	0.370120	8:-68316292	MsaT045088.1:CDS
ATTCTGACTTCCAGTGGGTT+GGG	0.370825	8:+68316257	None:intergenic
ACTTCAGCTAACTATCAATT+TGG	0.373900	8:-68314428	MsaT045088.1:CDS
CATTCTGACTTCCAGTGGGT+TGG	0.380743	8:+68316256	None:intergenic
GAACAAGTCTGTTCTATTCC+TGG	0.392480	8:+68314535	None:intergenic
ATGAAAATGCTTAATTGCCT+TGG	0.399936	8:+68314297	None:intergenic
TCAATGTTCTCGAAAGCATC+GGG	0.403367	8:-68316796	MsaT045088.1:CDS
AAGTGAAGCAGGTGCATGAC+TGG	0.407996	8:-68314358	MsaT045088.1:CDS
AACAAAACGATTCTAGGAAA+AGG	0.426966	8:-68314592	MsaT045088.1:CDS
GCACATTTGCAATATCATAA+AGG	0.434271	8:+68314868	None:intergenic
GATTCTAGGAAAAGGGCGTT+TGG	0.443366	8:-68314584	MsaT045088.1:CDS
TTAAGCTCCTGTAAAGCAAC+AGG	0.443519	8:+68316602	None:intergenic
ATACAAGAAAACCAGCATTC+AGG	0.443643	8:-68314667	MsaT045088.1:CDS
AAATGATAGTGGGCTATGTC+TGG	0.446224	8:-68314477	MsaT045088.1:CDS
CTGGCCTTGCAGCAAAAGTT+AGG	0.448266	8:-68314893	MsaT045088.1:intron
AAGACAATGGAAAATGATAG+TGG	0.463413	8:-68314488	MsaT045088.1:CDS
GATACAAAGGCAGGAGCAAA+AGG	0.470114	8:+68314404	None:intergenic
AAGCCTAGCCACCTGAATGC+TGG	0.489317	8:+68314656	None:intergenic
CTCTGAGAATAAAGTGAAGC+AGG	0.496045	8:-68314369	MsaT045088.1:CDS
ACAAAACGATTCTAGGAAAA+GGG	0.498407	8:-68314591	MsaT045088.1:CDS
ACACTTACTTGGTGCATAAG+AGG	0.500577	8:+68317092	None:intergenic
CCTGCCTTTGTATCCAAAGC+TGG	0.506957	8:-68314395	MsaT045088.1:CDS
GAATAGAACAGACTTGTTCA+TGG	0.511403	8:-68314531	MsaT045088.1:CDS
ACAACCTTGCTGCATCATCA+AGG	0.524403	8:+68315036	None:intergenic
AAGCCAAGAATCAGTATACC+TGG	0.526713	8:-68316643	MsaT045088.1:CDS
ACTTGGTGCATAAGAGGCCG+AGG	0.537885	8:+68317098	None:intergenic
AATCTCAATTCTTCACAAGA+AGG	0.539424	8:-68316454	MsaT045088.1:CDS
GTCAATGTTCTCGAAAGCAT+CGG	0.541833	8:-68316797	MsaT045088.1:CDS
TCACAAACATAAGCTTTGCC+AGG	0.545422	8:-68314553	MsaT045088.1:CDS
AAGAATCAGTATACCTGGAA+AGG	0.546799	8:-68316638	MsaT045088.1:CDS
GATGCAGCAAGGTTGTTGCT+TGG	0.550005	8:-68315029	MsaT045088.1:CDS
CCAGCTTTGGATACAAAGGC+AGG	0.550221	8:+68314395	None:intergenic
TCGTAAGTGTGGTGTCGAGA+AGG	0.550230	8:+68317136	None:intergenic
ATCGGAGGAAGAAGCAGCCA+TGG	0.550392	8:+68317162	None:intergenic
ATTGTCATTCTGACTTCCAG+TGG	0.553060	8:+68316251	None:intergenic
CTCCATGAACCTTATTGAGA+AGG	0.554046	8:-68314841	MsaT045088.1:intron
AAACCAGCATTCAGGTGGCT+AGG	0.559723	8:-68314659	MsaT045088.1:CDS
TTGTCATTCTGACTTCCAGT+GGG	0.561775	8:+68316252	None:intergenic
TCAGGTGGCTAGGCTTAAAA+GGG	0.561931	8:-68314649	MsaT045088.1:CDS
GTTCAGGTGTTGAGAGAAGA+CGG	0.579277	8:-68316832	MsaT045088.1:intron
TCCCAAACCACTGGAAGTCA+GGG	0.581818	8:-68316291	MsaT045088.1:CDS
TGATTCACCCTGACTTCCAG+TGG	0.587552	8:+68316284	None:intergenic
ATAATGAATACCTAATTTGG+CGG	0.587773	8:+68316931	None:intergenic
GCTTCCAGCTTTGGATACAA+AGG	0.589316	8:+68314391	None:intergenic
GGTATCGGATGAAGAGGATG+AGG	0.589877	8:-68316325	MsaT045088.1:CDS
AAAAGGGAAGACATTGAATG+AGG	0.592429	8:-68314633	MsaT045088.1:CDS
AGTGAAGCAGGTGCATGACT+GGG	0.592595	8:-68314357	MsaT045088.1:CDS
GATTTAGGTATCGGATGAAG+AGG	0.593110	8:-68316331	MsaT045088.1:intron
TTTGTTTACGATCGTAAGTG+TGG	0.596230	8:+68317125	None:intergenic
AATAGAACAGACTTGTTCAT+GGG	0.597678	8:-68314530	MsaT045088.1:CDS
GAATCATTATCCCAACCCAC+TGG	0.603753	8:-68316267	MsaT045088.1:CDS
CAACCTTGCTGCATCATCAA+GGG	0.604844	8:+68315037	None:intergenic
CTCAATTCTTCACAAGAAGG+TGG	0.607030	8:-68316451	MsaT045088.1:CDS
CTTGGTGCATAAGAGGCCGA+GGG	0.608163	8:+68317099	None:intergenic
AGACAATGGAAAATGATAGT+GGG	0.608266	8:-68314487	MsaT045088.1:CDS
GAATTGTTATCCCAAACCAC+TGG	0.620803	8:-68316300	MsaT045088.1:CDS
CTTTAAGAAGCAAAAGACAA+TGG	0.627305	8:-68314501	MsaT045088.1:CDS
TAAGTGTGGTGTCGAGAAGG+AGG	0.633826	8:+68317139	None:intergenic
GTGGTGTCGAGAAGGAGGAT+CGG	0.634729	8:+68317144	None:intergenic
AGGTGGCAATGAAGATGTGA+AGG	0.635108	8:-68316434	MsaT045088.1:intron
GTGTCGAGAAGGAGGATCGG+AGG	0.637595	8:+68317147	None:intergenic
CAAGAAAACCAGCATTCAGG+TGG	0.647844	8:-68314664	MsaT045088.1:CDS
CTTCTCAATAAGGTTCATGG+AGG	0.657302	8:+68314842	None:intergenic
TGCCCTCAGTATCAAAACCA+AGG	0.661299	8:-68314314	MsaT045088.1:CDS
AGAACAGACTTGTTCATGGG+TGG	0.672206	8:-68314527	MsaT045088.1:CDS
TGTCTGGAAGATGTGAAACA+AGG	0.683639	8:-68314461	MsaT045088.1:CDS
CAATGTTCTCGAAAGCATCG+GGG	0.717872	8:-68316795	MsaT045088.1:intron
TATCCCTTGATGATGCAGCA+AGG	0.728015	8:-68315040	MsaT045088.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	68314307	68317182	68314307	ID=MsaG045088
Chr8	mRNA	68314307	68317182	68314307	ID=MsaT045088.1;Parent=MsaG045088
Chr8	exon	68314307	68314702	68314307	ID=MsaT045088.1.exon10;Parent=MsaT045088.1
Chr8	CDS	68314307	68314702	68314307	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68314842	68314903	68314842	ID=MsaT045088.1.exon9;Parent=MsaT045088.1
Chr8	CDS	68314842	68314903	68314842	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68315000	68315072	68315000	ID=MsaT045088.1.exon8;Parent=MsaT045088.1
Chr8	CDS	68315000	68315072	68315000	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68315186	68315250	68315186	ID=MsaT045088.1.exon7;Parent=MsaT045088.1
Chr8	CDS	68315186	68315250	68315186	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68316211	68316346	68316211	ID=MsaT045088.1.exon6;Parent=MsaT045088.1
Chr8	CDS	68316211	68316346	68316211	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68316435	68316494	68316435	ID=MsaT045088.1.exon5;Parent=MsaT045088.1
Chr8	CDS	68316435	68316494	68316435	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68316601	68316678	68316601	ID=MsaT045088.1.exon4;Parent=MsaT045088.1
Chr8	CDS	68316601	68316678	68316601	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68316796	68316848	68316796	ID=MsaT045088.1.exon3;Parent=MsaT045088.1
Chr8	CDS	68316796	68316848	68316796	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68316942	68317009	68316942	ID=MsaT045088.1.exon2;Parent=MsaT045088.1
Chr8	CDS	68316942	68317009	68316942	ID=cds.MsaT045088.1;Parent=MsaT045088.1
Chr8	exon	68317100	68317182	68317100	ID=MsaT045088.1.exon1;Parent=MsaT045088.1
Chr8	CDS	68317100	68317182	68317100	ID=cds.MsaT045088.1;Parent=MsaT045088.1

Gene Sequence

>MsaT045088.1

ATGGCTGCTTCTTCCTCCGATCCTCCTTCTCGACACCACACTTACGATCGTAAACAAAAATCCCTCGGCCTCTTATGCACCAATTTCTTGAGCTTGTATAACAAAGACGATGTTCGTTTAATTGGTCTCGACGACGCTGCCGCCAAATTAGGTGTTGAGAGAAGACGGATCTATGATATTGTCAATGTTCTCGAAAGCATCGGGGTTCTTGCAAGAAAAGCCAAGAATCAGTATACCTGGAAAGGTTTTGCGGCAATTCCTGTTGCTTTACAGGAGCTTAAGGAAGAGGGTTTATGGCAGAATCTCAATTCTTCACAAGAAGGTGGCAATGAAGATGTGAAGGTATCGGATGAAGAGGATGAGGACGAATTGTTATCCCAAACCACTGGAAGTCAGGGTGAATCATTATCCCAACCCACTGGAAGTCAGAATGACAATCTAAACCCTAATTCAACTTTTCCCAGATCTTTGAAAAATGACAGAAGGGAAAAATCTCTGGCGCTGCTTACTCAGAATTTTGTCAAGCTCTTTGTCTGTTCTAACCTGGAAATGATATCCCTTGATGATGCAGCAAGGTTGTTGCTTGGAGATGCATATAATTCATCAACAATGAGAACAAAAGTTAGGCGCCTTTATGATATTGCAAATGTGCTAACCTCCATGAACCTTATTGAGAAGACCCATACCACAGATACAAGAAAACCAGCATTCAGGTGGCTAGGCTTAAAAGGGAAGACATTGAATGAGGCATCACTTTACAATTCAAAACAAAACGATTCTAGGAAAAGGGCGTTTGGAAATGATGTCACAAACATAAGCTTTGCCAGGAATAGAACAGACTTGTTCATGGGTGGTGACTTTAAGAAGCAAAAGACAATGGAAAATGATAGTGGGCTATGTCTGGAAGATGTGAAACAAGGCATAAAACAGACTTCAGCTAACTATCAATTTGGTCCTTTTGCTCCTGCCTTTGTATCCAAAGCTGGAAGCTCTGAGAATAAAGTGAAGCAGGTGCATGACTGGGAGAGTCTCGCTACTGAACATTGCCCTCAGTATCAAAACCAAGGCAATTAA

Protein sequence

>MsaT045088.1

MAASSSDPPSRHHTYDRKQKSLGLLCTNFLSLYNKDDVRLIGLDDAAAKLGVERRRIYDIVNVLESIGVLARKAKNQYTWKGFAAIPVALQELKEEGLWQNLNSSQEGGNEDVKVSDEEDEDELLSQTTGSQGESLSQPTGSQNDNLNPNSTFPRSLKNDRREKSLALLTQNFVKLFVCSNLEMISLDDAARLLLGDAYNSSTMRTKVRRLYDIANVLTSMNLIEKTHTTDTRKPAFRWLGLKGKTLNEASLYNSKQNDSRKRAFGNDVTNISFARNRTDLFMGGDFKKQKTMENDSGLCLEDVKQGIKQTSANYQFGPFAPAFVSKAGSSENKVKQVHDWESLATEHCPQYQNQGN*