AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045090 MtrunA17_Chr4g0059571 96.262 214 3 2 1 209 1 214 7.09e-144 399
MsaG045090 MtrunA17_Chr3g0118211 72.381 210 47 4 1 209 1 200 2.01e-98 284
MsaG045090 MtrunA17_Chr5g0414931 72.028 143 36 1 22 160 15 157 7.03e-65 200
MsaG045090 MtrunA17_Chr1g0170741 70.213 141 39 1 18 155 3 143 1.58e-63 195
MsaG045090 MtrunA17_Chr7g0256551 55.556 162 63 2 18 170 4 165 1.17e-56 179
MsaG045090 MtrunA17_Chr8g0352871 55.422 166 64 2 18 174 4 168 3.36e-55 173
MsaG045090 MtrunA17_Chr3g0082861 52.695 167 62 2 21 174 2 164 1.39e-51 164
MsaG045090 MtrunA17_Chr3g0128691 59.091 132 50 2 24 152 19 149 8.50e-49 156
MsaG045090 MtrunA17_Chr3g0124861 51.678 149 53 3 22 152 21 168 1.15e-44 148
MsaG045090 MtrunA17_Chr4g0021731 55.682 88 39 0 13 100 1 88 3.41e-25 94.4
MsaG045090 MtrunA17_Chr8g0345901 29.771 131 83 1 25 155 38 159 3.04e-15 73.6
MsaG045090 MtrunA17_Chr3g0123131 31.206 141 82 5 17 157 14 139 1.61e-14 71.6
MsaG045090 MtrunA17_Chr3g0127941 30.000 130 82 1 23 152 56 176 2.03e-14 71.2
MsaG045090 MtrunA17_Chr7g0275891 33.846 130 75 3 23 152 9 127 2.51e-14 70.5
MsaG045090 MtrunA17_Chr7g0275401 33.077 130 75 3 23 152 9 126 8.70e-14 68.6
MsaG045090 MtrunA17_Chr8g0376881 29.139 151 92 4 8 158 17 152 2.01e-13 68.2
MsaG045090 MtrunA17_Chr7g0276361 26.582 158 107 2 23 180 14 162 4.97e-13 67.4
MsaG045090 MtrunA17_Chr4g0030271 30.769 130 81 1 23 152 89 209 5.81e-13 67.0
MsaG045090 MtrunA17_Chr1g0149851 31.538 130 77 2 23 152 9 126 8.37e-13 66.6
MsaG045090 MtrunA17_Chr1g0149841 30.769 130 78 2 23 152 9 126 1.73e-12 65.5
MsaG045090 MtrunA17_Chr1g0181811 30.000 130 82 2 23 152 85 205 3.84e-12 64.7
MsaG045090 MtrunA17_Chr4g0061021 28.571 112 71 1 41 152 51 153 8.94e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045090 AT3G48100.1 73.548 155 40 1 23 176 25 179 1.88e-76 228
MsaG045090 AT5G62920.1 73.684 152 40 0 23 174 25 176 8.95e-74 221
MsaG045090 AT1G74890.1 72.973 148 37 2 22 166 17 164 8.52e-70 212
MsaG045090 AT1G59940.1 58.291 199 73 3 13 202 23 220 9.00e-69 210
MsaG045090 AT1G19050.1 64.671 167 46 3 3 163 2 161 1.91e-68 208
MsaG045090 AT1G10470.3 72.857 140 38 0 13 152 10 149 1.54e-67 207
MsaG045090 AT1G59940.2 58.291 199 73 3 13 202 115 312 2.22e-67 209
MsaG045090 AT1G10470.1 72.857 140 38 0 13 152 24 163 2.64e-67 207
MsaG045090 AT3G57040.2 59.333 150 51 2 18 157 4 153 2.95e-55 176
MsaG045090 AT3G57040.1 59.333 150 51 2 18 157 4 153 2.95e-55 176
MsaG045090 AT2G41310.1 60.417 144 49 1 21 156 7 150 5.25e-55 175
MsaG045090 AT2G40670.1 61.940 134 48 1 22 152 28 161 2.05e-54 171
MsaG045090 AT3G56380.1 62.121 132 49 1 22 152 19 150 5.73e-54 170
MsaG045090 AT2G40670.2 61.481 135 48 2 22 152 28 162 1.22e-52 167
MsaG045090 AT3G56380.2 61.654 133 49 2 22 152 19 151 3.68e-52 165
MsaG045090 AT1G10470.2 67.692 65 20 1 100 163 35 99 5.10e-22 89.0
MsaG045090 AT2G07440.1 64.286 56 20 0 101 156 9 64 2.45e-16 72.8
MsaG045090 AT1G67710.1 32.258 155 90 5 13 164 1 143 1.67e-15 74.7
MsaG045090 AT5G24470.1 30.075 133 84 1 23 155 50 173 2.28e-15 74.3
MsaG045090 AT5G07210.2 28.571 161 102 3 22 182 15 162 7.48e-15 72.8
MsaG045090 AT2G46790.3 28.462 130 84 1 23 152 37 157 1.54e-13 68.9
MsaG045090 AT5G60100.7 32.308 130 79 2 23 152 64 184 1.91e-13 68.6
MsaG045090 AT5G60100.6 32.308 130 79 2 23 152 64 184 1.91e-13 68.6
MsaG045090 AT5G60100.4 32.308 130 79 2 23 152 64 184 1.91e-13 68.6
MsaG045090 AT2G46790.1 28.462 130 84 1 23 152 37 157 2.13e-13 68.6
MsaG045090 AT5G60100.2 32.308 130 79 2 23 152 64 184 2.36e-13 68.6
MsaG045090 AT5G60100.5 32.308 130 79 2 23 152 64 184 3.11e-13 68.2
MsaG045090 AT5G60100.3 32.308 130 79 2 23 152 64 184 3.11e-13 68.2
MsaG045090 AT5G60100.1 32.308 130 79 2 23 152 64 184 3.11e-13 68.2
MsaG045090 AT2G25180.1 29.286 140 84 4 13 152 10 134 6.55e-13 67.4
MsaG045090 AT5G02810.1 29.231 130 83 2 23 152 78 198 1.42e-11 63.5
MsaG045090 AT3G16857.1 29.078 141 85 4 12 152 29 154 8.37e-11 61.2
MsaG045090 AT3G16857.2 29.078 141 85 4 12 152 29 154 8.51e-11 61.2

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCATGATGAAATCTTTC+AGG 0.203021 8:-68341825 None:intergenic
TTCACTAATTTGCAGGTGTT+TGG 0.354372 8:+68341747 MsaT045090.1:intron
TTGATGGAGGAATCACCTTC+AGG 0.429557 8:+68340183 MsaT045090.1:CDS
ATGACGGATTATTCCATGCC+TGG 0.447268 8:+68340590 MsaT045090.1:CDS
TATTCCATGCCTGGGATGAC+AGG 0.450141 8:+68340599 MsaT045090.1:CDS
GAGGAATCACCTTCAGGTGC+AGG 0.470142 8:+68340189 MsaT045090.1:CDS
CTTACATCTGACAATTTGAC+TGG 0.476820 8:-68341799 None:intergenic
GATGGAGAGAACAGTGATGT+TGG 0.500241 8:+68340440 MsaT045090.1:CDS
CAGTTTGTTATGGCTGCCGC+CGG 0.507838 8:+68340141 None:intergenic
TTGAAGATTTCTTCATGCAA+AGG 0.508242 8:+68340273 MsaT045090.1:CDS
GAAGGTTAATTTGATAATGA+CGG 0.519870 8:+68340574 MsaT045090.1:CDS
ACTAATTTGCAGGTGTTTGG+AGG 0.520826 8:+68341750 MsaT045090.1:intron
ATTTGCAGGTGTTTGGAGGA+AGG 0.521653 8:+68341754 MsaT045090.1:intron
AATTCATATCCTGTCATCCC+AGG 0.536780 8:-68340608 None:intergenic
GATAATGTTGATGGCGAAAG+TGG 0.540401 8:-68341976 None:intergenic
TGAATTACTCAAGAAAATCA+AGG 0.543151 8:+68340625 MsaT045090.1:CDS
TGCAATTCCCCTGCACCTGA+AGG 0.564770 8:-68340198 None:intergenic
TGACGGATTATTCCATGCCT+GGG 0.567665 8:+68340591 MsaT045090.1:CDS
AGGAATCACCTTCAGGTGCA+GGG 0.570190 8:+68340190 MsaT045090.1:CDS
ATATCCTGTCATCCCAGGCA+TGG 0.578908 8:-68340603 None:intergenic
TCAGACGACATGATCACCAC+TGG 0.582337 8:-68340813 None:intergenic
ATTCCTCCATCAAATTCGAC+CGG 0.585814 8:-68340173 None:intergenic
GTTATGGTGGCAGGGTGTGA+AGG 0.590843 8:+68340556 MsaT045090.1:intron
CTGAAAGATTTCATCATGAG+AGG 0.595036 8:+68341826 MsaT045090.1:CDS
CAGGTGTTTGGAGGAAGGAG+CGG 0.597585 8:+68341759 MsaT045090.1:intron
CATCATGAGAGGTGAAGTGA+AGG 0.602332 8:+68341837 MsaT045090.1:CDS
GGATCACATGAATGACGCAT+TGG 0.604562 8:-68341934 None:intergenic
ATTCGACCGGAAAATCTCGC+CGG 0.617787 8:-68340160 None:intergenic
GAGAATGTAGTTGATAGAGA+TGG 0.621435 8:-68341910 None:intergenic
GTGTTTGGAGGAAGGAGCGG+AGG 0.624937 8:+68341762 MsaT045090.1:CDS
TTTCCGGTCGAATTTGATGG+AGG 0.631772 8:+68340170 MsaT045090.1:CDS
GGAATCACCTTCAGGTGCAG+GGG 0.635571 8:+68340191 MsaT045090.1:CDS
CGACCGGAAAATCTCGCCGG+CGG 0.669618 8:-68340157 None:intergenic
ATGAGAGGTGAAGTGAAGGA+TGG 0.680662 8:+68341841 MsaT045090.1:CDS
AGTGGTGAGAACACTGACGA+TGG 0.682583 8:-68341958 None:intergenic
GGTGAGAACACTGACGATGG+CGG 0.683927 8:-68341955 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 68340150 68342020 68340150 ID=MsaG045090
Chr8 mRNA 68340150 68342020 68340150 ID=MsaT045090.1;Parent=MsaG045090
Chr8 exon 68340150 68340294 68340150 ID=MsaT045090.1.exon1;Parent=MsaT045090.1
Chr8 CDS 68340150 68340294 68340150 ID=cds.MsaT045090.1;Parent=MsaT045090.1
Chr8 exon 68340393 68340469 68340393 ID=MsaT045090.1.exon2;Parent=MsaT045090.1
Chr8 CDS 68340393 68340469 68340393 ID=cds.MsaT045090.1;Parent=MsaT045090.1
Chr8 exon 68340569 68340646 68340569 ID=MsaT045090.1.exon3;Parent=MsaT045090.1
Chr8 CDS 68340569 68340646 68340569 ID=cds.MsaT045090.1;Parent=MsaT045090.1
Chr8 exon 68340789 68340859 68340789 ID=MsaT045090.1.exon4;Parent=MsaT045090.1
Chr8 CDS 68340789 68340859 68340789 ID=cds.MsaT045090.1;Parent=MsaT045090.1
Chr8 exon 68341762 68342020 68341762 ID=MsaT045090.1.exon5;Parent=MsaT045090.1
Chr8 CDS 68341762 68342020 68341762 ID=cds.MsaT045090.1;Parent=MsaT045090.1
Gene Sequence

>MsaT045090.1

ATGGCTGCCGCCGGCGAGATTTTCCGGTCGAATTTGATGGAGGAATCACCTTCAGGTGCAGGGGAATTGCATGTGCTTGCTGTTGATGATAGTCTTGTTGATCGTAAAGTGATTGAACGTTTGTTGAAGATTTCTTCATGCAAAGTTACGGTTGTTGAGAGTGCAAGAAGAGCTCTCCAGTTTTTGGGATTGGATGGAGAGAACAGTGATGTTGGATTTGATGGTGTGAAGGTTAATTTGATAATGACGGATTATTCCATGCCTGGGATGACAGGATATGAATTACTCAAGAAAATCAAGGAATCTTCTGTTTTTAGAGAGATTCCAGTGGTGATCATGTCGTCTGAGAATGTTTTGACTCGAATTGATAGGTGTTTGGAGGAAGGAGCGGAGGATTTTTTGTTGAAGCCAGTCAAATTGTCAGATGTAAGACGCCTGAAAGATTTCATCATGAGAGGTGAAGTGAAGGATGGTGAGAAATGTTCTCTCAAAAGAATGCGATCATCAGATGATTGTAGTCCATCTCTATCAACTACATTCTCACCAATGCGTCATTCATGTGATCCGCCATCGTCAGTGTTCTCACCACTTTCGCCATCAACATTATCCTCTAAAAAATCTAGATTGTGA

Protein sequence

>MsaT045090.1

MAAAGEIFRSNLMEESPSGAGELHVLAVDDSLVDRKVIERLLKISSCKVTVVESARRALQFLGLDGENSDVGFDGVKVNLIMTDYSMPGMTGYELLKKIKESSVFREIPVVIMSSENVLTRIDRCLEEGAEDFLLKPVKLSDVRRLKDFIMRGEVKDGEKCSLKRMRSSDDCSPSLSTTFSPMRHSCDPPSSVFSPLSPSTLSSKKSRL*