AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045104


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045104 MtrunA17_Chr4g0059631 78.840 293 24 2 1 293 1 255 1.65e-166 462
MsaG045104 MtrunA17_Chr4g0022641 48.094 341 113 6 1 293 1 325 9.97e-96 287
MsaG045104 MtrunA17_Chr1g0212471 62.338 154 56 1 1 152 1 154 2.48e-62 195
MsaG045104 MtrunA17_Chr2g0303981 50.000 162 78 3 3 162 5 165 2.59e-47 157
MsaG045104 MtrunA17_Chr6g0457851 38.710 155 92 1 1 152 11 165 1.59e-35 130
MsaG045104 MtrunA17_Chr1g0212151 41.935 155 87 1 1 152 1 155 3.57e-35 129
MsaG045104 MtrunA17_Chr6g0449851 39.744 156 91 1 1 153 1 156 2.03e-34 127
MsaG045104 MtrunA17_Chr6g0449861 37.419 155 94 1 1 152 9 163 1.21e-29 115
MsaG045104 MtrunA17_Chr3g0107381 31.618 136 87 4 1 135 1 131 2.60e-14 70.9
MsaG045104 MtrunA17_Chr2g0283941 25.806 155 104 3 2 148 3 154 1.62e-12 65.9
MsaG045104 MtrunA17_Chr5g0432691 31.132 106 69 3 1 105 1 103 9.64e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045104 AT5G55690.1 28.402 169 110 2 1 165 1 162 4.62e-16 77.0
MsaG045104 AT5G55690.2 28.402 169 110 2 1 165 1 162 4.62e-16 77.0

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACACTTGTAAGGTCTTC+TGG 0.270047 8:+68512976 None:intergenic
TCCTGAGATATTTGATGTTA+AGG 0.348371 8:-68512911 MsaT045104.1:CDS
GCACTTTGGATGTATTTCAT+AGG 0.357011 8:+68513138 None:intergenic
GTCAACCTGATCAGTTTGTT+TGG 0.365476 8:-68512315 MsaT045104.1:CDS
AGCCCACTAAGTTGGTTTGA+TGG 0.416723 8:+68512616 None:intergenic
ACCATCTCACTTGTATAATC+AGG 0.418843 8:+68512565 None:intergenic
GATCAACAAGTTGACTAAGT+TGG 0.422061 8:+68512386 None:intergenic
CTCCACAAGCCCACTAAGTT+GGG 0.432529 8:+68512498 None:intergenic
ACAATCTCACTCATATAATC+AGG 0.433010 8:+68512454 None:intergenic
GCAAGGAGTTTACACTTGTA+AGG 0.440354 8:+68512968 None:intergenic
AGTTGGTTTGCTGATGAACT+TGG 0.448642 8:+68512403 None:intergenic
AGTTGGTTTGATGGTGAACT+AGG 0.459766 8:+68512625 None:intergenic
TCTCCACAAGCCCACTAAGT+TGG 0.464358 8:+68512497 None:intergenic
TCTTTCCAAACAAACTGATC+AGG 0.470431 8:+68512310 None:intergenic
CTAAAATGTGGATGCCAAGT+TGG 0.479466 8:+68512811 None:intergenic
TAAAATGTGGATGCCAAGTT+GGG 0.491000 8:+68512812 None:intergenic
GCTTGCTTTATTGTGTATGA+TGG 0.491871 8:-68513030 MsaT045104.1:CDS
TCATCATCAACCCAACTTAG+TGG 0.494369 8:-68512508 MsaT045104.1:CDS
TCCTTAACATCAAATATCTC+AGG 0.501707 8:+68512910 None:intergenic
TCTCCACAAGCCCACTAAGT+TGG 0.502348 8:+68512608 None:intergenic
CTTTGAAAATAAGACAGAAA+AGG 0.506269 8:-68512884 MsaT045104.1:CDS
TTGTAAGAAGTCTGGAGCAA+AGG 0.512867 8:-68513052 MsaT045104.1:CDS
AACTTGTTGATCTTGATGAC+TGG 0.515181 8:-68512375 MsaT045104.1:CDS
TCACCATCAAACCAACTTAG+TGG 0.519319 8:-68512619 MsaT045104.1:CDS
ATTGTGGATTTCACTAGTCT+TGG 0.527589 8:-68512436 MsaT045104.1:CDS
CATCATCAACCCAACTTAGT+GGG 0.531094 8:-68512507 MsaT045104.1:CDS
AACCCAACTTAGTGGGCTTG+TGG 0.535603 8:-68512500 MsaT045104.1:CDS
TATAATCAGGATTAACAACT+TGG 0.538229 8:+68512467 None:intergenic
AAACCAACTTAGTGGGCTTG+TGG 0.552156 8:-68512611 MsaT045104.1:CDS
AGGTCTTCTGGCCATGTCGT+AGG 0.557005 8:+68512988 None:intergenic
TGATTATATGAGTGAGATTG+TGG 0.565961 8:-68512452 MsaT045104.1:CDS
TCATCCAAGTTACTAAAATG+TGG 0.567439 8:+68512799 None:intergenic
ATGGTGGATTTCACAAGTCT+TGG 0.570027 8:-68512547 MsaT045104.1:CDS
TGGTTTGCTGATGAACTTGG+AGG 0.570787 8:+68512406 None:intergenic
ACTTTCAAACAAAGAAAGCA+TGG 0.571291 8:-68513099 MsaT045104.1:CDS
TCAGTAAGATCTACCCAACT+TGG 0.575658 8:-68512825 MsaT045104.1:CDS
TATAATCAGGATCAACAACT+TGG 0.576059 8:+68512578 None:intergenic
CACCATCAAACCAACTTAGT+GGG 0.576425 8:-68512618 MsaT045104.1:CDS
AGTGGGCTTGTGGAGAGGAA+TGG 0.577682 8:-68512601 MsaT045104.1:CDS
ATTCTGATACTCTTGAAGCA+AGG 0.580425 8:+68512951 None:intergenic
AACTTAGTGGGCTTGTGGAG+AGG 0.584533 8:-68512606 MsaT045104.1:CDS
TGATTATACAAGTGAGATGG+TGG 0.591946 8:-68512563 MsaT045104.1:CDS
ACAAATAGAAACTAGCACCT+TGG 0.593239 8:-68512692 MsaT045104.1:CDS
AGTTGGGTTGATGATGAACT+AGG 0.593899 8:+68512514 None:intergenic
TGGTTTGATGGTGAACTAGG+AGG 0.598871 8:+68512628 None:intergenic
AATCATCTCACATATATCCA+AGG 0.609547 8:+68512675 None:intergenic
TGGATAAATCAATTAGATGA+CGG 0.620408 8:-68512355 MsaT045104.1:CDS
AAACAAAGATTAAGAAAGTG+CGG 0.626571 8:-68512858 MsaT045104.1:CDS
TCCTGATTATACAAGTGAGA+TGG 0.678910 8:-68512566 MsaT045104.1:CDS
TGGGTTGATGATGAACTAGG+AGG 0.698401 8:+68512517 None:intergenic
ATAATATTGAACCTACGACA+TGG 0.717032 8:-68512999 MsaT045104.1:CDS
AAGTTGGGTAGATCTTACTG+AGG 0.750378 8:+68512827 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 68512297 68513178 68512297 ID=MsaG045104
Chr8 mRNA 68512297 68513178 68512297 ID=MsaT045104.1;Parent=MsaG045104
Chr8 exon 68512297 68513178 68512297 ID=MsaT045104.1.exon1;Parent=MsaT045104.1
Chr8 CDS 68512297 68513178 68512297 ID=cds.MsaT045104.1;Parent=MsaT045104.1
Gene Sequence

>MsaT045104.1

ATGGGTCGCGCAAAAATACCTATGAAATACATCCAAAGTGCGAAATCTCGCAAAATAACTTTCAAACAAAGAAAGCATGGATTAGAAAAGAAAATTTCTGAATTTTGTAAGAAGTCTGGAGCAAAGGCTTGCTTTATTGTGTATGATGGTGATCATAATAATATTGAACCTACGACATGGCCAGAAGACCTTACAAGTGTAAACTCCTTGCTTCAAGAGTATCAGAATCAAAAGATTGAGAAAACTCCTGAGATATTTGATGTTAAGGACTACTTTGAAAATAAGACAGAAAAGGTTGAAACAAAGATTAAGAAAGTGCGGAGAGATATCCTCAGTAAGATCTACCCAACTTGGCATCCACATTTTAGTAACTTGGATGAGAATCAACTCAGAGACTTCATTGCTACAATAAATGCTAAGATTCAAGCTTGCAATCACAAAATTAGCATGTTGAAAAATATGCAACAAATAGAAACTAGCACCTTGGATATATGTGAGATGATTGATTTTTCTGATCTTGGTGATTTTCCTCCTAGTTCACCATCAAACCAACTTAGTGGGCTTGTGGAGAGGAATGGCCAAGTTGTTGATCCTGATTATACAAGTGAGATGGTGGATTTCACAAGTCTTGGTGATTTGCCTCCTAGTTCATCATCAACCCAACTTAGTGGGCTTGTGGAGATGAGTGACCAAGTTGTTAATCCTGATTATATGAGTGAGATTGTGGATTTCACTAGTCTTGGTGATTTGCCTCCAAGTTCATCAGCAAACCAACTTAGTCAACTTGTTGATCTTGATGACTGGATAAATCAATTAGATGACGGTGTTTTGTGCTGGGATTGTCAACCTGATCAGTTTGTTTGGAAAGATCTTTCTTTTTAA

Protein sequence

>MsaT045104.1

MGRAKIPMKYIQSAKSRKITFKQRKHGLEKKISEFCKKSGAKACFIVYDGDHNNIEPTTWPEDLTSVNSLLQEYQNQKIEKTPEIFDVKDYFENKTEKVETKIKKVRRDILSKIYPTWHPHFSNLDENQLRDFIATINAKIQACNHKISMLKNMQQIETSTLDICEMIDFSDLGDFPPSSPSNQLSGLVERNGQVVDPDYTSEMVDFTSLGDLPPSSSSTQLSGLVEMSDQVVNPDYMSEIVDFTSLGDLPPSSSANQLSQLVDLDDWINQLDDGVLCWDCQPDQFVWKDLSF*