AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045131


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045131 MtrunA17_Chr4g0059631 78.498 293 25 2 1 293 1 255 3.28e-164 456
MsaG045131 MtrunA17_Chr4g0022641 47.507 341 115 6 1 293 1 325 6.88e-94 283
MsaG045131 MtrunA17_Chr1g0212471 62.338 154 56 1 1 152 1 154 6.51e-62 194
MsaG045131 MtrunA17_Chr2g0303981 50.000 162 78 3 3 162 5 165 4.74e-47 156
MsaG045131 MtrunA17_Chr1g0212151 42.581 155 86 1 1 152 1 155 5.34e-36 132
MsaG045131 MtrunA17_Chr6g0457851 38.710 155 92 1 1 152 11 165 6.30e-36 132
MsaG045131 MtrunA17_Chr6g0449851 39.744 156 91 1 1 153 1 156 8.25e-35 128
MsaG045131 MtrunA17_Chr6g0449861 37.419 155 94 1 1 152 9 163 5.50e-30 116
MsaG045131 MtrunA17_Chr3g0107381 32.353 136 86 4 1 135 1 131 4.19e-15 72.8
MsaG045131 MtrunA17_Chr2g0283941 26.452 155 103 3 2 148 3 154 2.55e-13 68.2
MsaG045131 MtrunA17_Chr5g0432691 32.075 106 68 3 1 105 1 103 1.72e-11 61.6
MsaG045131 MtrunA17_Chr3g0107461 30.252 119 75 4 1 114 1 116 9.10e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045131 AT5G55690.1 28.402 169 110 2 1 165 1 162 7.04e-16 76.3
MsaG045131 AT5G55690.2 28.402 169 110 2 1 165 1 162 7.04e-16 76.3
MsaG045131 AT1G22590.2 35.849 106 64 3 1 105 1 103 7.74e-11 60.1

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACACTTGTAAGGTCTTC+TGG 0.270047 8:-68959388 None:intergenic
TCCTGAGATATTTGATGTTA+AGG 0.348371 8:+68959453 MsaT045131.1:CDS
GCACTTTGGATGTATTTCAT+AGG 0.357011 8:-68959226 None:intergenic
GTCAACCTGATGAGTTTGTT+TGG 0.385592 8:+68960049 MsaT045131.1:CDS
AGCCCACTAAGTTGGTTTGA+TGG 0.416723 8:-68959748 None:intergenic
ACCATCTCACTTGTATAATC+AGG 0.418843 8:-68959799 None:intergenic
GATCAACAAGTTGACTAAGT+TGG 0.431119 8:-68959978 None:intergenic
ACAATCTCACTCATATAATC+AGG 0.433010 8:-68959910 None:intergenic
GCAAGGAGTTTACACTTGTA+AGG 0.440354 8:-68959396 None:intergenic
AGTTGGTTTGATGGTGAACT+AGG 0.459766 8:-68959739 None:intergenic
AGTTGGTTTGTTGATGAACT+TGG 0.473151 8:-68959961 None:intergenic
CTAAAATGTGGATGCCAAGT+TGG 0.479466 8:-68959553 None:intergenic
TATTTCCAAACAAACTCATC+AGG 0.484604 8:-68960054 None:intergenic
TAAAATGTGGATGCCAAGTT+GGG 0.491000 8:-68959552 None:intergenic
GCTTGCTTTATTGTGTATGA+TGG 0.491871 8:+68959334 MsaT045131.1:CDS
TATAATCAGGATTAGCAACT+TGG 0.493452 8:-68959897 None:intergenic
TCATCATCAAACCAACTTAG+TGG 0.501564 8:+68959856 MsaT045131.1:CDS
TCCTTAACATCAAATATCTC+AGG 0.501707 8:-68959454 None:intergenic
TCTCCACAAGCCCACTAAGT+TGG 0.502348 8:-68959756 None:intergenic
CTTTGAAAATAAGATAGAAA+AGG 0.507369 8:+68959480 MsaT045131.1:CDS
TCTCCACAAGCCCACTAAGT+TGG 0.508925 8:-68959867 None:intergenic
TTGTAAGAAGTCTGGAGCAA+AGG 0.512867 8:+68959312 MsaT045131.1:CDS
AACTTGTTGATCTCGATGAC+TGG 0.514836 8:+68959989 MsaT045131.1:CDS
TCACCATCAAACCAACTTAG+TGG 0.519319 8:+68959745 MsaT045131.1:CDS
ATTGTGGATTTCAATAGTCT+TGG 0.530083 8:+68959928 MsaT045131.1:CDS
AGTTGGTTTGATGATGAACT+AGG 0.530891 8:-68959850 None:intergenic
AAACCAACTTAGTGGGCTTG+TGG 0.552156 8:+68959753 MsaT045131.1:CDS
AAACCAACTTAGTGGGCTTG+TGG 0.552156 8:+68959864 MsaT045131.1:CDS
CATCATCAAACCAACTTAGT+GGG 0.554520 8:+68959857 MsaT045131.1:CDS
AGGTCTTCTGGCCATGTCGT+AGG 0.557005 8:-68959376 None:intergenic
TGATTATATGAGTGAGATTG+TGG 0.565961 8:+68959912 MsaT045131.1:CDS
TCATCCAAGTTACTAAAATG+TGG 0.567439 8:-68959565 None:intergenic
ATGGTGGATTTCACAAGTCT+TGG 0.570027 8:+68959817 MsaT045131.1:CDS
ACTTTCAAACAAAGAAAGCA+TGG 0.571291 8:+68959265 MsaT045131.1:CDS
TCAGTAAGATCTACCCAACT+TGG 0.575658 8:+68959539 MsaT045131.1:CDS
TATAATCAGGATCAACAACT+TGG 0.576059 8:-68959786 None:intergenic
CACCATCAAACCAACTTAGT+GGG 0.576425 8:+68959746 MsaT045131.1:CDS
AGTGGGCTTGTGGAGAGGAA+TGG 0.577682 8:+68959763 MsaT045131.1:CDS
ATTCTGATACTCTTGAAGCA+AGG 0.580425 8:-68959413 None:intergenic
AACTTAGTGGGCTTGTGGAG+AGG 0.584533 8:+68959758 MsaT045131.1:CDS
TGGTTTGTTGATGAACTTGG+AGG 0.590987 8:-68959958 None:intergenic
TGATTATACAAGTGAGATGG+TGG 0.591946 8:+68959801 MsaT045131.1:CDS
ACAAATAGAAACTAGCACCT+TGG 0.593239 8:+68959672 MsaT045131.1:CDS
TGGTTTGATGGTGAACTAGG+AGG 0.598871 8:-68959736 None:intergenic
AATCATCTCACATATATCCA+AGG 0.609547 8:-68959689 None:intergenic
TGGATAAATCAACTAGATGA+TGG 0.621105 8:+68960009 MsaT045131.1:CDS
AAACAAAGATTAAGAAAGTG+CGG 0.626571 8:+68959506 MsaT045131.1:CDS
TGGTTTGATGATGAACTAGG+AGG 0.662339 8:-68959847 None:intergenic
TCCTGATTATACAAGTGAGA+TGG 0.678910 8:+68959798 MsaT045131.1:CDS
ATAATATTGAACCTACGACA+TGG 0.717032 8:+68959365 MsaT045131.1:CDS
AAGTTGGGTAGATCTTACTG+AGG 0.750378 8:-68959537 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 68959208 68960089 68959208 ID=MsaG045131
Chr8 mRNA 68959208 68960089 68959208 ID=MsaT045131.1;Parent=MsaG045131
Chr8 exon 68959208 68960089 68959208 ID=MsaT045131.1.exon1;Parent=MsaT045131.1
Chr8 CDS 68959208 68960089 68959208 ID=cds.MsaT045131.1;Parent=MsaT045131.1
Gene Sequence

>MsaT045131.1

ATGGGTCGCGCAAAAATACCTATGAAATACATCCAAAGTGCGAAATCTCGCAAAATAACTTTCAAACAAAGAAAGCATGGATTAGAAAAGAAAATTTCTGAATTTTGTAAGAAGTCTGGAGCAAAGGCTTGCTTTATTGTGTATGATGGTGATCATAATAATATTGAACCTACGACATGGCCAGAAGACCTTACAAGTGTAAACTCCTTGCTTCAAGAGTATCAGAATCAAAAGATTGAGAAAACTCCTGAGATATTTGATGTTAAGGACTACTTTGAAAATAAGATAGAAAAGGTTGAAACAAAGATTAAGAAAGTGCGGAGAGATATCCTCAGTAAGATCTACCCAACTTGGCATCCACATTTTAGTAACTTGGATGAGAATCAACTCAGAGACTTCATTGCTACAATAAATGCTAAGATTCAAGCTTGCAATCACAAAATTAGCATGTTGAAAAATATGCAACAAATAGAAACTAGCACCTTGGATATATGTGAGATGATTGATTTTTCTGATCTTGGTGATTTTCCTCCTAGTTCACCATCAAACCAACTTAGTGGGCTTGTGGAGAGGAATGGCCAAGTTGTTGATCCTGATTATACAAGTGAGATGGTGGATTTCACAAGTCTTGGTGATTTGCCTCCTAGTTCATCATCAAACCAACTTAGTGGGCTTGTGGAGATGAGTGACCAAGTTGCTAATCCTGATTATATGAGTGAGATTGTGGATTTCAATAGTCTTGGTGATTTGCCTCCAAGTTCATCAACAAACCAACTTAGTCAACTTGTTGATCTCGATGACTGGATAAATCAACTAGATGATGGTGTTTTGTATTGGGATTGTCAACCTGATGAGTTTGTTTGGAAATATCTTGCTTTTTAA

Protein sequence

>MsaT045131.1

MGRAKIPMKYIQSAKSRKITFKQRKHGLEKKISEFCKKSGAKACFIVYDGDHNNIEPTTWPEDLTSVNSLLQEYQNQKIEKTPEIFDVKDYFENKIEKVETKIKKVRRDILSKIYPTWHPHFSNLDENQLRDFIATINAKIQACNHKISMLKNMQQIETSTLDICEMIDFSDLGDFPPSSPSNQLSGLVERNGQVVDPDYTSEMVDFTSLGDLPPSSSSNQLSGLVEMSDQVANPDYMSEIVDFNSLGDLPPSSSTNQLSQLVDLDDWINQLDDGVLYWDCQPDEFVWKYLAF*