AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045135

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045135 MtrunA17_Chr4g0059701 92.517 294 11 5 1 290 1 287 1.44e-175 486
MsaG045135 MtrunA17_Chr5g0415191 56.757 185 47 5 6 175 19 185 9.04e-50 167
MsaG045135 MtrunA17_Chr4g0055931 57.746 142 52 4 18 155 25 162 2.24e-47 161
MsaG045135 MtrunA17_Chr2g0331031 43.684 190 67 5 3 167 15 189 8.67e-43 150
MsaG045135 MtrunA17_Chr3g0092621 78.571 84 17 1 88 170 84 167 1.45e-39 136
MsaG045135 MtrunA17_Chr6g0488471 51.020 147 56 4 13 152 24 161 6.72e-38 135
MsaG045135 MtrunA17_Chr3g0104071 61.702 94 32 1 76 165 135 228 1.54e-34 127
MsaG045135 MtrunA17_Chr8g0389241 73.239 71 18 1 82 152 99 168 3.55e-34 125
MsaG045135 MtrunA17_Chr4g0062741 71.622 74 21 0 81 154 131 204 5.03e-34 125
MsaG045135 MtrunA17_Chr1g0162081 51.261 119 53 1 81 194 115 233 5.93e-34 125
MsaG045135 MtrunA17_Chr1g0210471 59.223 103 40 2 81 181 117 219 1.51e-33 122
MsaG045135 MtrunA17_Chr5g0429521 65.854 82 28 0 77 158 121 202 1.06e-32 122
MsaG045135 MtrunA17_Chr7g0241761 49.138 116 56 1 76 188 128 243 5.95e-32 120
MsaG045135 MtrunA17_Chr5g0436501 61.176 85 31 1 76 158 113 197 5.21e-30 114
MsaG045135 MtrunA17_Chr6g0452791 81.356 59 11 0 88 146 80 138 2.15e-27 104
MsaG045135 MtrunA17_Chr3g0135671 53.933 89 38 1 70 155 73 161 2.91e-27 105
MsaG045135 MtrunA17_Chr5g0409841 32.500 160 78 4 48 177 27 186 6.06e-24 97.1
MsaG045135 MtrunA17_Chr5g0409831 45.631 103 49 3 70 166 60 161 7.62e-22 91.7
MsaG045135 MtrunA17_Chr5g0440731 44.944 89 43 2 82 164 99 187 3.81e-20 88.2
MsaG045135 MtrunA17_Chr5g0440741 48.148 81 36 2 90 164 102 182 1.29e-19 86.7
MsaG045135 MtrunA17_Chr8g0365011 40.164 122 51 4 90 189 94 215 8.47e-17 78.6
MsaG045135 MtrunA17_Chr8g0364731 34.940 166 74 5 97 229 10 174 3.87e-16 75.1
MsaG045135 MtrunA17_Chr1g0192061 51.429 70 30 2 90 156 63 131 3.03e-15 72.4
MsaG045135 MtrunA17_Chr5g0432921 36.752 117 64 4 59 173 86 194 1.51e-14 71.2
MsaG045135 MtrunA17_Chr3g0113121 48.611 72 30 1 90 154 97 168 2.55e-13 67.8
MsaG045135 MtrunA17_Chr5g0432941 43.023 86 41 3 90 173 115 194 5.22e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045135 AT1G74840.1 56.579 228 78 8 8 229 16 228 3.24e-60 192
MsaG045135 AT1G74840.2 63.542 192 60 6 8 194 16 202 1.22e-59 190
MsaG045135 AT1G19000.2 55.769 208 69 5 1 194 4 202 2.60e-53 176
MsaG045135 AT1G19000.1 55.769 208 69 5 1 194 4 202 2.60e-53 176
MsaG045135 AT5G47390.1 54.658 161 63 3 18 173 25 180 7.96e-52 174
MsaG045135 AT1G70000.1 61.988 171 43 6 5 168 23 178 2.92e-50 167
MsaG045135 AT1G70000.2 61.988 171 43 6 5 168 23 178 2.92e-50 167
MsaG045135 AT3G16350.1 44.550 211 87 7 18 207 49 250 1.20e-39 142
MsaG045135 AT5G58900.1 51.304 115 50 1 76 190 129 237 3.29e-34 126
MsaG045135 AT1G49010.1 72.973 74 20 0 81 154 127 200 4.03e-34 126
MsaG045135 AT2G38090.1 58.763 97 40 0 65 161 118 214 7.22e-34 125
MsaG045135 AT5G04760.1 65.116 86 29 1 77 161 88 173 1.50e-33 122
MsaG045135 AT5G61620.1 39.362 188 83 6 4 177 22 192 4.55e-33 124
MsaG045135 AT5G08520.1 70.270 74 22 0 81 154 111 184 8.93e-33 122
MsaG045135 AT5G56840.1 57.282 103 32 3 63 164 73 164 6.15e-32 118
MsaG045135 AT5G01200.1 64.865 74 26 0 81 154 139 212 2.62e-29 112
MsaG045135 AT5G05790.2 61.250 80 31 0 75 154 118 197 2.83e-29 112
MsaG045135 AT5G05790.1 61.250 80 31 0 75 154 118 197 2.83e-29 112
MsaG045135 AT3G11280.2 61.250 80 31 0 75 154 114 193 3.43e-29 112
MsaG045135 AT3G11280.1 61.250 80 31 0 75 154 114 193 3.43e-29 112
MsaG045135 AT3G10580.2 60.000 70 26 1 84 153 91 158 4.96e-23 95.5
MsaG045135 AT3G10580.1 60.000 70 26 1 84 153 91 158 8.03e-23 95.9
MsaG045135 AT4G09450.1 50.000 94 46 1 69 161 71 164 1.16e-22 93.2
MsaG045135 AT5G23650.1 58.333 72 30 0 82 153 114 185 2.38e-22 95.5
MsaG045135 AT3G10590.1 46.512 86 45 1 76 161 99 183 3.41e-19 84.0

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGTTCGCGGTCGCGGTTTC+TGG 0.130907 8:-69010681 None:intergenic
AACTCATATACTGACATAAA+AGG 0.288173 8:-69011655 None:intergenic
ATGCCATGTTTCAATAATAA+TGG 0.319591 8:+69011916 MsaT045135.1:CDS
ATCAGACACAATGGAAGCTT+TGG 0.326101 8:-69011791 None:intergenic
GGAAGAGCACAAGCTGTTCT+TGG 0.356599 8:+69010824 MsaT045135.1:CDS
TTGACCTGTTCTTCCTCCAT+TGG 0.357105 8:-69011571 None:intergenic
AATTGAATAGATTGTTGGTT+TGG 0.361654 8:-69011744 None:intergenic
GTCATCGGCGGAGGCGTAAC+CGG 0.383661 8:-69010639 None:intergenic
CTCGTTTACGTTCGCGGTCG+CGG 0.408987 8:-69010688 None:intergenic
TGGAACCTCGTTTACGTTCG+CGG 0.412917 8:-69010694 None:intergenic
AATTGAATTGAATAGATTGT+TGG 0.420883 8:-69011749 None:intergenic
TTGGTTGGATTGCAGAAAGT+TGG 0.429183 8:+69010843 MsaT045135.1:CDS
TTTATGTCAGTATATGAGTT+GGG 0.444220 8:+69011658 MsaT045135.1:CDS
ACCGCAGACTCCACCGTCTC+CGG 0.449707 8:+69010470 MsaT045135.1:CDS
AATTCCAATGGAGGAAGAAC+AGG 0.463202 8:+69011567 MsaT045135.1:CDS
TCAGACACAATGGAAGCTTT+GGG 0.470464 8:-69011790 None:intergenic
AGAAAGTTGGAAAAGGTGAT+TGG 0.477943 8:+69010856 MsaT045135.1:CDS
TGGAGGGTCAATGGATGAAC+TGG 0.479134 8:-69011827 None:intergenic
GGAAAGTGTTGGAGGGTCAA+TGG 0.479524 8:-69011836 None:intergenic
GAGCACAAGCTGTTCTTGGT+TGG 0.483963 8:+69010828 MsaT045135.1:CDS
CCAATGGAAGAACTCACTCT+TGG 0.488703 8:+69011697 MsaT045135.1:CDS
AGGAACGGCGTCATCGGCGG+AGG 0.501551 8:-69010648 None:intergenic
TCCGGCGAATTCATGCTCTT+CGG 0.514098 8:+69010488 MsaT045135.1:CDS
CCAAGAGTGAGTTCTTCCAT+TGG 0.515535 8:-69011697 None:intergenic
GAGCATGAATTCGCCGGAGA+CGG 0.519327 8:-69010483 None:intergenic
TTTCCTTCTCGTCCGATCAA+AGG 0.521674 8:+69011866 MsaT045135.1:CDS
GACCCTCCAACACTTTCCCT+CGG 0.522607 8:+69011841 MsaT045135.1:CDS
TTGGGATCTGAAACAACAAA+AGG 0.525424 8:-69011772 None:intergenic
TGGATCAGGGATTCCGTGGA+CGG 0.525462 8:+69010800 MsaT045135.1:intron
TGATGATGTCTTCCTTTGAT+CGG 0.527840 8:-69011878 None:intergenic
ATGATGTTGTTGTTGTCTTG+AGG 0.532951 8:-69010581 None:intergenic
GGTGGTGATATCAAAGAGAC+TGG 0.540239 8:-69010988 None:intergenic
TTGTGCTCTTCCTCCGTCCA+CGG 0.546086 8:-69010813 None:intergenic
GGATTGCAGAAAGTTGGAAA+AGG 0.553347 8:+69010849 MsaT045135.1:CDS
AGTCCGAGGGAAAGTGTTGG+AGG 0.562456 8:-69011844 None:intergenic
TTCGCGGTCGCGGTTTCTGG+CGG 0.563265 8:-69010678 None:intergenic
CATGAATTCGCCGGAGACGG+TGG 0.565729 8:-69010480 None:intergenic
TCAGGTATCTGCAATTCCAA+TGG 0.575185 8:+69011555 MsaT045135.1:intron
CTTCCTTTGATCGGACGAGA+AGG 0.575650 8:-69011869 None:intergenic
GCCGGAGACGGTGGAGTCTG+CGG 0.589898 8:-69010471 None:intergenic
TTGAGGAACGGCGTCATCGG+CGG 0.592610 8:-69010651 None:intergenic
TATCAAAGAGACTGGATCTA+CGG 0.592657 8:-69010980 None:intergenic
CGTTAATGAGAGTACTCCAA+TGG 0.593695 8:+69011681 MsaT045135.1:CDS
TCCGAAGAGCATGAATTCGC+CGG 0.594213 8:-69010489 None:intergenic
AGACAACAACAACATCATCA+AGG 0.604820 8:+69010586 MsaT045135.1:CDS
TCTCCATTATTATTGAAACA+TGG 0.609269 8:-69011919 None:intergenic
AACAAAGACGTTATCACCGC+CGG 0.612643 8:+69010620 MsaT045135.1:CDS
GTGATTGGAGAGGTATCTCA+AGG 0.614661 8:+69010871 MsaT045135.1:CDS
AATGGATGAACTGGAACTAG+AGG 0.623042 8:-69011818 None:intergenic
GAAGAACTCACTCTTGGACA+AGG 0.624406 8:+69011703 MsaT045135.1:CDS
ATCGGCGGAGGCGTAACCGG+CGG 0.626944 8:-69010636 None:intergenic
CAGAAAAGTTACCGTGTCGG+TGG 0.629241 8:-69011006 None:intergenic
GGCGATTGAGATTACTTCGG+CGG 0.629813 8:-69010953 None:intergenic
CGCGACCGCGAACGTAAACG+AGG 0.635630 8:+69010689 MsaT045135.1:CDS
CTTTGATATCACCACCGACA+CGG 0.638033 8:+69010995 MsaT045135.1:CDS
GTCCGAGGGAAAGTGTTGGA+GGG 0.638496 8:-69011843 None:intergenic
GAAAGTCCGAGGGAAAGTGT+TGG 0.638503 8:-69011847 None:intergenic
TGCCAGAAAAGTTACCGTGT+CGG 0.638678 8:-69011009 None:intergenic
CGGACGAGAAGGAAAGTCCG+AGG 0.639721 8:-69011858 None:intergenic
GACGGCGATTGAGATTACTT+CGG 0.641222 8:-69010956 None:intergenic
GGTATCTGCAATTCCAATGG+AGG 0.648622 8:+69011558 MsaT045135.1:intron
AGAGGTGATATCAGACACAA+TGG 0.652354 8:-69011800 None:intergenic
GGACGAGAAGGAAAGTCCGA+GGG 0.654533 8:-69011857 None:intergenic
GTTGGAAAAGGTGATTGGAG+AGG 0.659248 8:+69010861 MsaT045135.1:CDS
GGAGACGGTGGAGTCTGCGG+TGG 0.660956 8:-69010468 None:intergenic
ATCAGGGATTCCGTGGACGG+AGG 0.665588 8:+69010803 MsaT045135.1:intron
GAAAACAAGAACACCTACGC+AGG 0.669459 8:+69010902 MsaT045135.1:CDS
AGAGACTGGATCTACGGCGA+CGG 0.684123 8:-69010974 None:intergenic
GCATGACTTGCAACCTGCGT+AGG 0.689624 8:-69010915 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 69010452 69011963 69010452 ID=MsaG045135
Chr8 mRNA 69010452 69011963 69010452 ID=MsaT045135.1;Parent=MsaG045135
Chr8 exon 69010452 69010710 69010452 ID=MsaT045135.1.exon1;Parent=MsaT045135.1
Chr8 CDS 69010452 69010710 69010452 ID=cds.MsaT045135.1;Parent=MsaT045135.1
Chr8 exon 69010808 69011016 69010808 ID=MsaT045135.1.exon2;Parent=MsaT045135.1
Chr8 CDS 69010808 69011016 69010808 ID=cds.MsaT045135.1;Parent=MsaT045135.1
Chr8 exon 69011559 69011963 69011559 ID=MsaT045135.1.exon3;Parent=MsaT045135.1
Chr8 CDS 69011559 69011963 69011559 ID=cds.MsaT045135.1;Parent=MsaT045135.1
Gene Sequence

>MsaT045135.1

ATGTCTTCTGCTTCAACCACCGCAGACTCCACCGTCTCCGGCGAATTCATGCTCTTCGGAGTTAGAGTCGTCGTTGATTCTATGAGAAAGAGTGTCAGTATGAACAATCTCTCACAATACGAACAACAACCTCAAGACAACAACAACATCATCAAGGATGATAATATCAACAAAGACGTTATCACCGCCGGTTACGCCTCCGCCGATGACGCCGTTCCTCAAAACTCCGCCAGAAACCGCGACCGCGAACGTAAACGAGGGATTCCGTGGACGGAGGAAGAGCACAAGCTGTTCTTGGTTGGATTGCAGAAAGTTGGAAAAGGTGATTGGAGAGGTATCTCAAGGAACTATGTGAAAACAAGAACACCTACGCAGGTTGCAAGTCATGCTCAAAAGTACTTTCTCCGCCGAAGTAATCTCAATCGCCGTCGCCGTAGATCCAGTCTCTTTGATATCACCACCGACACGGTATCTGCAATTCCAATGGAGGAAGAACAGGTCAAGAATCAAGATAATGTTTCACAGTGCCCTGCAGCCCCCGAAGCTAGAAAAATCAATGGATTTCCTTTTATGTCAGTATATGAGTTGGGCGTTAATGAGAGTACTCCAATGGAAGAACTCACTCTTGGACAAGGAAATGTGAAACACAATTTCCAAACCAACAATCTATTCAATTCAATTCCTTTTGTTGTTTCAGATCCCAAAGCTTCCATTGTGTCTGATATCACCTCTAGTTCCAGTTCATCCATTGACCCTCCAACACTTTCCCTCGGACTTTCCTTCTCGTCCGATCAAAGGAAGACATCATCAACACATTTACATGCAATGCCATGTTTCAATAATAATGGAGATAACATCATAAGTGTTGCTTAA
Protein sequence

>MsaT045135.1

MSSASTTADSTVSGEFMLFGVRVVVDSMRKSVSMNNLSQYEQQPQDNNNIIKDDNINKDVITAGYASADDAVPQNSARNRDRERKRGIPWTEEEHKLFLVGLQKVGKGDWRGISRNYVKTRTPTQVASHAQKYFLRRSNLNRRRRRSSLFDITTDTVSAIPMEEEQVKNQDNVSQCPAAPEARKINGFPFMSVYELGVNESTPMEELTLGQGNVKHNFQTNNLFNSIPFVVSDPKASIVSDITSSSSSSIDPPTLSLGLSFSSDQRKTSSTHLHAMPCFNNNGDNIISVA*