Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045150 | XP_013457865.1 | 99.142 | 233 | 2 | 0 | 1 | 233 | 67 | 299 | 1.61e-169 | 479 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045150 | sp|Q9STS6|LBD27_ARATH | 30.417 | 240 | 119 | 8 | 1 | 196 | 93 | 328 | 9.82e-16 | 78.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045150 | tr|A0A072URD2|A0A072URD2_MEDTR | 99.142 | 233 | 2 | 0 | 1 | 233 | 67 | 299 | 7.67e-170 | 479 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG045150 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045150 | MtrunA17_Chr4g0059901 | 98.995 | 199 | 2 | 0 | 1 | 199 | 67 | 265 | 2.42e-147 | 411 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG045150 | AT3G47870.1 | 30.417 | 240 | 119 | 8 | 1 | 196 | 93 | 328 | 9.98e-17 | 78.2 |
Find 41 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8:-69230639 | MsaT045150.1:CDS |
| GTGACTTCACAGTTAGAATT+AGG | 0.247178 | 8:-69230469 | MsaT045150.1:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8:-69230528 | MsaT045150.1:CDS |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8:-69230579 | MsaT045150.1:CDS |
| AGCAATGCAACCTCATAAAT+TGG | 0.298388 | 8:-69230209 | MsaT045150.1:CDS |
| GCAATCATACATTTATTCTA+AGG | 0.300515 | 8:-69230089 | MsaT045150.1:CDS |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8:-69230638 | MsaT045150.1:CDS |
| GGGCACTGAAGCCACAAATT+AGG | 0.358880 | 8:+69230259 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8:+69230155 | None:intergenic |
| CTGCTATTGTTATTTGTGTA+AGG | 0.395645 | 8:+69230238 | None:intergenic |
| TTGTTATAAGTCTGTGGTTG+TGG | 0.430309 | 8:+69230400 | None:intergenic |
| CATCATGAGGTAACTGTTGT+TGG | 0.444844 | 8:+69230494 | None:intergenic |
| ATGCTACAAATCCTAATTTG+TGG | 0.446980 | 8:-69230270 | MsaT045150.1:CDS |
| AAAGAGTGCGAGCGCATTGA+AGG | 0.447294 | 8:+69230432 | None:intergenic |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8:-69230597 | MsaT045150.1:CDS |
| TCTGAGGTAACCAATTTATG+AGG | 0.490694 | 8:+69230199 | None:intergenic |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8:-69230572 | MsaT045150.1:CDS |
| CAATAACTCCTCCCTCTACA+TGG | 0.493365 | 8:-69230305 | MsaT045150.1:CDS |
| TGCTATTGTTATTTGTGTAA+GGG | 0.508900 | 8:+69230239 | None:intergenic |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8:+69230651 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8:-69230296 | MsaT045150.1:CDS |
| GTAATCCACACTATTGCTGT+TGG | 0.537932 | 8:+69230327 | None:intergenic |
| ATCATACATTTATTCTAAGG+AGG | 0.554320 | 8:-69230086 | MsaT045150.1:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8:+69229680 | None:intergenic |
| TTATTGTTGTTATAAGTCTG+TGG | 0.569885 | 8:+69230394 | None:intergenic |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8:+69230607 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8:+69230333 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8:+69230169 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8:-69230619 | MsaT045150.1:CDS |
| TTCACAGTTAGAATTAGGAA+TGG | 0.586596 | 8:-69230464 | MsaT045150.1:CDS |
| ATCCTTTGAATCCATGTAGA+GGG | 0.590065 | 8:+69230294 | None:intergenic |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8:-69230332 | MsaT045150.1:CDS |
| CATCCTTTGAATCCATGTAG+AGG | 0.632560 | 8:+69230293 | None:intergenic |
| CCACTATCATCTGTCCAACA+AGG | 0.637452 | 8:-69230169 | MsaT045150.1:CDS |
| TGATAGTGGCTGCGACTCTG+AGG | 0.643276 | 8:+69230183 | None:intergenic |
| CTAAGGAGGCTTATGAATCG+AGG | 0.657710 | 8:-69230072 | MsaT045150.1:intron |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8:+69229675 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8:+69230297 | None:intergenic |
| TGTGAAGTCACATCATCATG+AGG | 0.679884 | 8:+69230481 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8:-69230608 | MsaT045150.1:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8:+69230358 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 69229684 | 69230671 | 69229684 | ID=MsaG045150 |
| Chr8 | mRNA | 69229684 | 69230671 | 69229684 | ID=MsaT045150.1;Parent=MsaG045150 |
| Chr8 | exon | 69229684 | 69229786 | 69229684 | ID=MsaT045150.1.exon2;Parent=MsaT045150.1 |
| Chr8 | CDS | 69229684 | 69229786 | 69229684 | ID=cds.MsaT045150.1;Parent=MsaT045150.1 |
| Chr8 | exon | 69230073 | 69230671 | 69230073 | ID=MsaT045150.1.exon1;Parent=MsaT045150.1 |
| Chr8 | CDS | 69230073 | 69230671 | 69230073 | ID=cds.MsaT045150.1;Parent=MsaT045150.1 |
| Gene Sequence |
| Protein sequence |
