AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045161

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045161 MtrunA17_Chr4g0060031 94.755 286 10 3 4 285 53 337 0.0 538
MsaG045161 MtrunA17_Chr5g0415941 48.630 292 129 9 4 283 42 324 3.79e-73 227
MsaG045161 MtrunA17_Chr8g0362751 41.522 289 113 8 4 285 47 286 3.62e-52 172
MsaG045161 MtrunA17_Chr6g0450991 45.374 227 92 6 3 226 31 228 2.28e-46 157
MsaG045161 MtrunA17_Chr5g0416391 53.846 143 56 3 17 150 45 186 1.60e-38 136
MsaG045161 MtrunA17_Chr8g0362151 54.815 135 53 3 24 150 52 186 2.75e-38 136
MsaG045161 MtrunA17_Chr3g0123691 58.559 111 46 0 37 147 51 161 1.40e-36 132
MsaG045161 MtrunA17_Chr6g0453021 45.399 163 77 3 1 155 54 212 4.46e-36 130
MsaG045161 MtrunA17_Chr3g0119291 62.857 105 39 0 45 149 58 162 9.34e-36 129
MsaG045161 MtrunA17_Chr8g0381741 60.784 102 40 0 45 146 53 154 2.96e-35 129
MsaG045161 MtrunA17_Chr7g0217111 43.684 190 93 6 32 208 64 252 8.37e-34 124
MsaG045161 MtrunA17_Chr7g0265521 48.148 135 50 4 45 179 62 176 4.20e-30 113
MsaG045161 MtrunA17_Chr1g0179581 57.426 101 35 2 35 135 18 110 3.37e-29 107
MsaG045161 MtrunA17_Chr3g0127511 55.789 95 42 0 48 142 40 134 1.38e-28 109
MsaG045161 MtrunA17_Chr8g0346251 47.778 90 47 0 53 142 48 137 1.20e-24 99.4
MsaG045161 MtrunA17_Chr5g0403701 48.544 103 46 1 46 148 53 148 9.00e-22 90.5
MsaG045161 MtrunA17_Chr5g0403691 51.923 104 48 2 46 148 53 155 1.96e-21 90.1
MsaG045161 MtrunA17_Chr4g0055851 50.926 108 44 2 31 138 107 205 6.41e-21 90.1
MsaG045161 MtrunA17_Chr5g0399491 44.118 136 57 3 3 138 105 221 4.37e-19 85.1
MsaG045161 MtrunA17_Chr1g0175651 46.341 123 55 3 20 138 124 239 9.99e-19 84.3
MsaG045161 MtrunA17_Chr2g0308611 50.000 90 45 0 44 133 198 287 5.00e-18 82.8
MsaG045161 MtrunA17_Chr7g0258831 47.115 104 53 1 30 133 146 247 5.74e-18 82.0
MsaG045161 MtrunA17_Chr8g0335131 51.613 93 38 2 46 138 146 231 2.05e-17 80.5
MsaG045161 MtrunA17_Chr4g0054421 46.154 104 49 1 35 138 116 212 3.08e-17 79.7
MsaG045161 MtrunA17_Chr1g0151971 42.727 110 52 1 24 133 119 217 2.52e-16 77.4
MsaG045161 MtrunA17_Chr5g0400781 44.792 96 53 0 38 133 115 210 9.81e-16 75.5
MsaG045161 MtrunA17_Chr3g0135331 48.611 72 37 0 46 117 145 216 2.24e-14 72.0
MsaG045161 MtrunA17_Chr4g0044091 45.161 93 44 1 46 138 65 150 2.43e-14 70.1
MsaG045161 MtrunA17_Chr4g0071411 44.444 90 43 1 49 138 91 173 4.49e-14 69.7
MsaG045161 MtrunA17_Chr2g0299031 40.909 88 52 0 46 133 82 169 1.38e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045161 AT3G01470.1 44.954 218 88 6 4 218 33 221 3.87e-44 151
MsaG045161 AT4G40060.1 42.308 234 115 7 21 251 31 247 2.34e-40 142
MsaG045161 AT3G01220.1 58.621 116 44 1 44 159 85 196 1.16e-36 132
MsaG045161 AT2G22430.1 63.636 99 36 0 44 142 60 158 1.30e-36 132
MsaG045161 AT5G15150.1 65.263 95 33 0 44 138 113 207 2.44e-36 132
MsaG045161 AT1G69780.1 54.918 122 50 1 34 150 73 194 6.85e-36 130
MsaG045161 AT5G65310.2 62.626 99 37 0 44 142 52 150 8.73e-36 130
MsaG045161 AT5G65310.1 62.626 99 37 0 44 142 70 168 1.06e-35 130
MsaG045161 AT1G26960.1 48.872 133 67 1 10 141 34 166 1.39e-31 118
MsaG045161 AT1G27045.1 47.445 137 65 1 22 151 37 173 6.44e-31 115
MsaG045161 AT1G27045.5 53.846 117 53 1 34 149 12 128 1.89e-30 113
MsaG045161 AT1G27045.3 53.846 117 53 1 34 149 12 128 1.89e-30 113
MsaG045161 AT1G27045.2 53.846 117 53 1 34 149 4 120 2.19e-30 112
MsaG045161 AT1G27045.4 49.194 124 56 1 22 138 37 160 3.49e-29 112
MsaG045161 AT2G46680.1 46.903 113 60 0 37 149 23 135 4.64e-28 108
MsaG045161 AT2G18550.1 48.120 133 54 3 16 148 39 156 5.30e-28 107
MsaG045161 AT3G61890.1 51.485 101 48 1 49 149 33 132 2.56e-27 106
MsaG045161 AT2G46680.2 51.020 98 48 0 37 134 23 120 1.15e-26 105
MsaG045161 AT4G36740.2 50.485 103 44 1 46 148 56 151 1.43e-25 101
MsaG045161 AT4G36740.1 50.485 103 44 1 46 148 55 150 1.44e-25 101
MsaG045161 AT2G36610.1 40.909 154 65 5 18 154 34 178 1.92e-25 100
MsaG045161 AT5G03790.1 45.528 123 54 3 46 162 77 192 2.41e-25 101
MsaG045161 AT5G03790.2 53.763 93 36 1 46 138 77 162 3.56e-25 99.8
MsaG045161 AT5G66700.1 48.454 97 43 1 46 142 71 160 9.04e-23 94.4
MsaG045161 AT5G06710.1 48.624 109 40 2 30 138 183 275 1.41e-18 84.7
MsaG045161 AT3G60390.1 46.281 121 54 3 18 138 138 247 1.51e-18 84.3
MsaG045161 AT5G53980.1 46.667 90 48 0 46 135 11 100 4.14e-18 79.7
MsaG045161 AT5G47370.1 48.077 104 47 1 35 138 119 215 1.15e-17 81.3
MsaG045161 AT2G44910.1 51.613 93 38 1 46 138 163 248 1.59e-17 81.6
MsaG045161 AT4G16780.1 45.133 113 47 2 26 138 117 214 1.76e-17 80.9
MsaG045161 AT4G17460.1 47.619 105 47 2 35 138 123 220 6.12e-17 79.3
MsaG045161 AT2G22800.1 43.750 96 54 0 38 133 105 200 1.65e-15 75.1
MsaG045161 AT2G01430.1 42.478 113 51 3 31 138 121 224 9.37e-15 73.2
MsaG045161 AT4G37790.1 43.810 105 52 2 34 138 114 211 2.03e-14 72.0
MsaG045161 AT5G06710.4 41.837 98 46 2 30 125 183 271 8.79e-14 70.5
MsaG045161 AT2G01430.2 46.575 73 32 2 31 98 121 191 6.53e-12 63.9
MsaG045161 AT1G70920.1 43.011 93 46 1 46 138 69 154 2.06e-11 62.4
MsaG045161 AT1G70920.2 43.011 93 46 1 46 138 39 124 2.14e-11 62.0

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCTCAACAATTGACAATT+TGG 0.241624 8:+69454042 None:intergenic
TTGAATAGGTTCAAGATCTA+TGG 0.281332 8:-69454958 MsaT045161.1:intron
TGAGAGACGCCGTTTCTTCT+CGG 0.306253 8:+69454827 None:intergenic
ATCATCAAGCTCTTTGGCCT+TGG 0.324405 8:-69453760 MsaT045161.1:CDS
AGCCACGGCAAGTTGCTATT+TGG 0.343826 8:-69454710 MsaT045161.1:CDS
TTCAGCAAGGAGTGACATAT+TGG 0.347228 8:-69453987 MsaT045161.1:CDS
TGTCTTCTTCATCTTGTGAT+AGG 0.364063 8:+69453882 None:intergenic
CATGATAATTCATCAAGTTA+TGG 0.374938 8:-69453800 MsaT045161.1:CDS
AAGATCATCATCAAGCTCTT+TGG 0.376736 8:-69453766 MsaT045161.1:CDS
ACCAAGGTCTTTAGCTAGCT+TGG 0.382164 8:+69454737 None:intergenic
GGATTTGATCTTGACGAAAA+CGG 0.405214 8:-69454882 MsaT045161.1:CDS
ACCAAAGGTTTATTCATTGT+TGG 0.412212 8:+69454091 None:intergenic
ATAATCCTTCTCAAGCTGTT+TGG 0.413395 8:+69454659 None:intergenic
TGAAACAAAGGAATTCTTGC+AGG 0.414573 8:-69454116 MsaT045161.1:CDS
ACCAACAATGAATAAACCTT+TGG 0.422093 8:-69454092 MsaT045161.1:CDS
AAGGAAGGAAAAGGTGCAAC+AGG 0.438515 8:-69454917 MsaT045161.1:CDS
ACCTTCTCAAAGAGAAAGAT+AGG 0.463134 8:-69454590 MsaT045161.1:CDS
CGAAAACGGCGAGGATGAGA+TGG 0.475286 8:-69454868 MsaT045161.1:CDS
AGGTGCAACAGGTCTTTCTT+CGG 0.489691 8:-69454906 MsaT045161.1:CDS
TTTGGTTGATTCAGTTTCTG+AGG 0.492605 8:-69454074 MsaT045161.1:CDS
GGTAGCAAGCCTAACTGAAA+AGG 0.493946 8:-69454185 MsaT045161.1:CDS
ACCTATCTTTCTCTTTGAGA+AGG 0.495244 8:+69454589 None:intergenic
AAGACCTTGGTTTGCAGCCA+CGG 0.500948 8:-69454725 MsaT045161.1:CDS
ATCCAAATTGTCAATTGTTG+AGG 0.508800 8:-69454044 MsaT045161.1:CDS
TTCATCAATCCGAGAAGAAA+CGG 0.510950 8:-69454836 MsaT045161.1:CDS
TTGGTTGATTCAGTTTCTGA+GGG 0.528561 8:-69454073 MsaT045161.1:CDS
TCTTGAGAAAAGCTTTGAAG+AGG 0.530902 8:-69454787 MsaT045161.1:CDS
GAAAGATAGGTTACAATCTG+AGG 0.533848 8:-69454577 MsaT045161.1:intron
AAGATGTTCAAGGAAGGAAA+AGG 0.536023 8:-69454926 MsaT045161.1:CDS
TTCAAAACCGTCGTGCAAGA+TGG 0.537462 8:-69454686 MsaT045161.1:CDS
ATTTGAACATGAATATCAAT+CGG 0.545738 8:-69453906 MsaT045161.1:CDS
ACCAAGCTAGCTAAAGACCT+TGG 0.547629 8:-69454738 MsaT045161.1:CDS
GATTCAGTTTCTGAGGGTGA+AGG 0.550989 8:-69454067 MsaT045161.1:CDS
ATATTCATGTTCAAATACAT+AGG 0.565080 8:+69453913 None:intergenic
TGCAACAGGTCTTTCTTCGG+AGG 0.566461 8:-69454903 MsaT045161.1:CDS
ACTGATGGAGTGTTAGAGAC+AGG 0.581498 8:-69453944 MsaT045161.1:CDS
GACAGCCCACGTTACACTGA+TGG 0.581501 8:-69453959 MsaT045161.1:CDS
CTTGCCGTGGCTGCAAACCA+AGG 0.588928 8:+69454721 None:intergenic
GAAGAAACGGCGTCTCTCAG+TGG 0.590083 8:-69454823 MsaT045161.1:CDS
TTGATGAATTATCATGTGGC+GGG 0.592126 8:+69453807 None:intergenic
CTTGATGAATTATCATGTGG+CGG 0.594488 8:+69453806 None:intergenic
ATCAAGTTATGGATTTCAAG+AGG 0.600284 8:-69453789 MsaT045161.1:CDS
TGAATTATCATGTGGCGGGT+CGG 0.601580 8:+69453811 None:intergenic
ACAAGGTGAAAGTGAAACAA+AGG 0.601903 8:-69454128 MsaT045161.1:CDS
TTGAAGATATTAGTTCAGCA+AGG 0.615993 8:-69454000 MsaT045161.1:CDS
TGGTCTTCCATCTTGCACGA+CGG 0.616539 8:+69454679 None:intergenic
GAAGACCAAACAGCTTGAGA+AGG 0.620125 8:-69454664 MsaT045161.1:CDS
CAAGAGGGAAAATTCAAACA+AGG 0.630062 8:-69454145 MsaT045161.1:CDS
AACACTCCATCAGTGTAACG+TGG 0.635850 8:+69453953 None:intergenic
TAACTTGATGAATTATCATG+TGG 0.637883 8:+69453803 None:intergenic
AACCAAATAGCAACTTGCCG+TGG 0.641928 8:+69454708 None:intergenic
CATATCATTTAATAAGACCA+AGG 0.657999 8:+69453743 None:intergenic
ACTTGCAAGAGAGAAACAAG+AGG 0.662555 8:-69454161 MsaT045161.1:CDS
TCAGAAACTGAATCAACCAA+AGG 0.671154 8:+69454076 None:intergenic
TGATCTTGACGAAAACGGCG+AGG 0.684795 8:-69454877 MsaT045161.1:CDS
CTTGCAAGAGAGAAACAAGA+GGG 0.688671 8:-69454160 MsaT045161.1:CDS
ACACTCCATCAGTGTAACGT+GGG 0.696078 8:+69453954 None:intergenic
ACAACAAGCTTGAACCAGAG+AGG 0.699556 8:-69454764 MsaT045161.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 69453751 69455646 69453751 ID=MsaG045161
Chr8 mRNA 69453751 69455646 69453751 ID=MsaT045161.1;Parent=MsaG045161
Chr8 exon 69453751 69454206 69453751 ID=MsaT045161.1.exon3;Parent=MsaT045161.1
Chr8 CDS 69453751 69454206 69453751 ID=cds.MsaT045161.1;Parent=MsaT045161.1
Chr8 exon 69454578 69454972 69454578 ID=MsaT045161.1.exon2;Parent=MsaT045161.1
Chr8 CDS 69454578 69454972 69454578 ID=cds.MsaT045161.1;Parent=MsaT045161.1
Chr8 exon 69455640 69455646 69455640 ID=MsaT045161.1.exon1;Parent=MsaT045161.1
Chr8 CDS 69455640 69455646 69455640 ID=cds.MsaT045161.1;Parent=MsaT045161.1
Gene Sequence

>MsaT045161.1

ATGGCACGTTCAAGATCTATGGTGAGTTTTGAAGATGTTCAAGGAAGGAAAAGGTGCAACAGGTCTTTCTTCGGAGGATTTGATCTTGACGAAAACGGCGAGGATGAGATGGATGAGTACTTTCATCAATCCGAGAAGAAACGGCGTCTCTCAGTGGATCAAGTTCAGTTTCTTGAGAAAAGCTTTGAAGAGGACAACAAGCTTGAACCAGAGAGGAAAACCAAGCTAGCTAAAGACCTTGGTTTGCAGCCACGGCAAGTTGCTATTTGGTTTCAAAACCGTCGTGCAAGATGGAAGACCAAACAGCTTGAGAAGGATTATGATTCTCTTAATGATAGTTATGAGTCTCTTAAGACAGATTATGACAACCTTCTCAAAGAGAAAGATAGGTTACAATCTGAGGTAGCAAGCCTAACTGAAAAGGTACTTGCAAGAGAGAAACAAGAGGGAAAATTCAAACAAGGTGAAAGTGAAACAAAGGAATTCTTGCAGGAACCAACAATGAATAAACCTTTGGTTGATTCAGTTTCTGAGGGTGAAGGATCCAAATTGTCAATTGTTGAGGCTTCTAATAACAATAACAAACTTGAAGATATTAGTTCAGCAAGGAGTGACATATTGGATTGTGACAGCCCACGTTACACTGATGGAGTGTTAGAGACAGGTGATTCTTCCTATGTATTTGAACATGAATATCAATCGGACCTATCACAAGATGAAGAAGACAATTTATTGCCTCCTTACATCTTTACAAAACTTGAAGATGTGAATTACTCCGACCCGCCACATGATAATTCATCAAGTTATGGATTTCAAGAGGAAGATCATCATCAAGCTCTTTGGCCTTGGTCTTATTAA
Protein sequence

>MsaT045161.1

MARSRSMVSFEDVQGRKRCNRSFFGGFDLDENGEDEMDEYFHQSEKKRRLSVDQVQFLEKSFEEDNKLEPERKTKLAKDLGLQPRQVAIWFQNRRARWKTKQLEKDYDSLNDSYESLKTDYDNLLKEKDRLQSEVASLTEKVLAREKQEGKFKQGESETKEFLQEPTMNKPLVDSVSEGEGSKLSIVEASNNNNKLEDISSARSDILDCDSPRYTDGVLETGDSSYVFEHEYQSDLSQDEEDNLLPPYIFTKLEDVNYSDPPHDNSSSYGFQEEDHHQALWPWSY*