AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045178

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045178 MtrunA17_Chr4g0060271 96.272 617 18 2 1 616 1 613 0.0 1217
MsaG045178 MtrunA17_Chr7g0216891 40.071 564 263 19 68 599 38 558 5.57e-96 305
MsaG045178 MtrunA17_Chr2g0322391 34.298 484 219 17 59 527 157 556 6.72e-56 199
MsaG045178 MtrunA17_Chr3g0099641 36.158 354 152 9 64 402 87 381 3.61e-52 186
MsaG045178 MtrunA17_Chr4g0051181 35.940 537 260 22 1 526 10 473 4.04e-51 184
MsaG045178 MtrunA17_Chr2g0288871 35.417 480 196 16 70 527 143 530 2.62e-49 180
MsaG045178 MtrunA17_Chr4g0075201 37.798 336 154 8 70 405 120 400 6.22e-49 178
MsaG045178 MtrunA17_Chr7g0270831 71.845 103 29 0 233 335 190 292 3.32e-47 171
MsaG045178 MtrunA17_Chr2g0302391 49.143 175 77 3 225 399 181 343 1.84e-43 161
MsaG045178 MtrunA17_Chr4g0025061 51.948 154 61 3 244 397 345 485 8.87e-42 160
MsaG045178 MtrunA17_Chr4g0025051 63.810 105 38 0 244 348 320 424 1.40e-40 156
MsaG045178 MtrunA17_Chr4g0024391 28.283 495 240 16 82 527 12 440 1.43e-37 145
MsaG045178 MtrunA17_Chr4g0001361 48.214 112 53 3 242 351 143 251 3.78e-25 106
MsaG045178 MtrunA17_Chr1g0150281 50.000 94 47 0 242 335 123 216 9.45e-25 104
MsaG045178 MtrunA17_Chr1g0150271 45.918 98 51 1 240 335 80 177 1.22e-24 103
MsaG045178 MtrunA17_Chr3g0136961 63.333 60 22 0 258 317 137 196 2.21e-24 103
MsaG045178 MtrunA17_Chr5g0432161 45.946 111 52 3 213 319 374 480 3.70e-24 107
MsaG045178 MtrunA17_Chr3g0106491 52.564 78 36 1 242 319 348 424 1.34e-23 104
MsaG045178 MtrunA17_Chr3g0106491 49.206 63 30 2 259 321 198 258 3.30e-13 72.4
MsaG045178 MtrunA17_Chr5g0445311 34.637 179 99 4 157 335 279 439 7.75e-23 102
MsaG045178 MtrunA17_Chr5g0445311 50.820 61 28 2 259 319 200 258 1.28e-11 67.4
MsaG045178 MtrunA17_Chr4g0004911 50.633 79 38 1 242 320 319 396 9.28e-23 102
MsaG045178 MtrunA17_Chr4g0004911 48.333 60 30 1 260 319 185 243 7.10e-13 71.2
MsaG045178 MtrunA17_Chr7g0243891 52.564 78 36 1 242 319 357 433 1.57e-22 101
MsaG045178 MtrunA17_Chr7g0243891 51.613 62 28 2 260 321 217 276 4.38e-12 68.9
MsaG045178 MtrunA17_Chr3g0101131 51.899 79 37 1 241 319 397 474 3.32e-22 100
MsaG045178 MtrunA17_Chr3g0101131 49.180 61 29 2 259 319 243 301 2.08e-11 67.0
MsaG045178 MtrunA17_Chr2g0333851 43.478 92 41 1 239 319 138 229 3.52e-22 97.8
MsaG045178 MtrunA17_Chr5g0418191 36.842 171 101 3 182 350 245 410 3.53e-22 99.8
MsaG045178 MtrunA17_Chr5g0418191 50.746 67 30 3 253 319 146 209 1.18e-13 73.6
MsaG045178 MtrunA17_Chr7g0218081 42.478 113 60 2 226 334 373 484 5.24e-22 100
MsaG045178 MtrunA17_Chr7g0218081 42.222 90 49 3 230 319 200 286 3.33e-12 69.3
MsaG045178 MtrunA17_Chr3g0079691 46.392 97 47 2 227 319 459 554 8.70e-22 100
MsaG045178 MtrunA17_Chr3g0079691 39.000 100 56 4 220 319 244 338 2.83e-12 69.7
MsaG045178 MtrunA17_Chr4g0042831 50.000 80 39 1 244 323 263 341 2.18e-21 96.7
MsaG045178 MtrunA17_Chr4g0042831 30.909 165 101 5 259 421 145 298 6.40e-12 67.8
MsaG045178 MtrunA17_Chr7g0263531 50.649 77 37 1 247 323 349 424 2.61e-21 97.1
MsaG045178 MtrunA17_Chr7g0263531 45.679 81 41 3 254 334 185 262 1.61e-13 73.2
MsaG045178 MtrunA17_Chr7g0249311 50.000 78 38 1 242 319 375 451 3.59e-21 98.2
MsaG045178 MtrunA17_Chr7g0249311 48.485 66 32 2 257 322 177 240 3.14e-11 66.6
MsaG045178 MtrunA17_Chr7g0249301 52.703 74 34 1 246 319 279 351 4.52e-21 95.5
MsaG045178 MtrunA17_Chr7g0249301 49.206 63 30 2 257 319 60 120 2.70e-11 65.9
MsaG045178 MtrunA17_Chr1g0194481 40.000 125 69 4 226 348 128 248 1.07e-20 93.6
MsaG045178 MtrunA17_Chr7g0270191 39.167 120 60 3 226 337 104 218 1.99e-20 92.0
MsaG045178 MtrunA17_Chr4g0074001 51.250 80 38 1 241 320 341 419 4.24e-20 94.0
MsaG045178 MtrunA17_Chr5g0419241 77.778 45 10 0 243 287 61 105 4.83e-20 85.9
MsaG045178 MtrunA17_Chr4g0051881 39.423 104 62 1 246 349 165 267 7.25e-20 91.3
MsaG045178 MtrunA17_Chr8g0335811 46.988 83 43 1 235 317 130 211 7.86e-20 89.0
MsaG045178 MtrunA17_Chr5g0447701 45.882 85 45 1 244 328 237 320 1.59e-19 91.3
MsaG045178 MtrunA17_Chr2g0294231 48.052 77 39 1 243 319 187 262 3.58e-19 89.7
MsaG045178 MtrunA17_Chr3g0129361 47.368 76 39 1 242 317 127 201 4.16e-19 86.7
MsaG045178 MtrunA17_Chr4g0068541 47.436 78 40 1 243 320 192 268 7.72e-19 89.4
MsaG045178 MtrunA17_Chr1g0150801 49.315 73 36 1 244 316 87 158 8.59e-19 84.0
MsaG045178 MtrunA17_Chr5g0401821 55.224 67 28 1 254 320 196 260 1.85e-18 85.9
MsaG045178 MtrunA17_Chr5g0443131 48.649 74 37 1 246 319 144 216 2.28e-18 86.3
MsaG045178 MtrunA17_Chr4g0053281 57.627 59 25 0 260 318 267 325 2.56e-18 86.7
MsaG045178 MtrunA17_Chr8g0379461 55.385 65 29 0 259 323 76 140 2.96e-18 84.7
MsaG045178 MtrunA17_Chr5g0418781 38.462 117 64 3 221 336 92 201 2.97e-18 85.5
MsaG045178 MtrunA17_Chr1g0188941 43.590 78 43 1 242 319 34 110 3.16e-18 80.9
MsaG045178 MtrunA17_Chr7g0270051 52.703 74 32 1 259 332 53 123 3.48e-18 85.1
MsaG045178 MtrunA17_Chr7g0224801 48.571 70 35 1 249 318 83 151 6.20e-18 81.6
MsaG045178 MtrunA17_Chr2g0319961 49.367 79 40 0 250 328 71 149 1.22e-17 83.2
MsaG045178 MtrunA17_Chr8g0383351 46.753 77 39 2 242 317 142 217 1.24e-17 82.8
MsaG045178 MtrunA17_Chr8g0383251 42.857 77 43 1 246 322 95 170 1.36e-17 81.3
MsaG045178 MtrunA17_Chr2g0289181 41.818 110 53 3 247 347 4 111 3.91e-17 81.3
MsaG045178 MtrunA17_Chr3g0129321 46.753 77 40 1 246 322 105 180 4.83e-17 80.1
MsaG045178 MtrunA17_Chr1g0184691 53.448 58 27 0 260 317 294 351 9.09e-17 82.4
MsaG045178 MtrunA17_Chr7g0228941 48.571 70 33 2 248 316 42 109 9.13e-17 77.0
MsaG045178 MtrunA17_Chr1g0147881 52.542 59 28 0 260 318 263 321 1.00e-16 82.0
MsaG045178 MtrunA17_Chr2g0284321 53.333 60 28 0 260 319 36 95 1.36e-16 79.3
MsaG045178 MtrunA17_Chr8g0386241 34.615 104 64 2 225 325 66 168 1.91e-16 77.8
MsaG045178 MtrunA17_Chr7g0248721 50.000 74 35 2 243 316 125 196 3.24e-16 78.2
MsaG045178 MtrunA17_Chr3g0139021 40.870 115 48 4 220 316 259 371 3.76e-16 80.9
MsaG045178 MtrunA17_Chr2g0293481 46.667 75 40 0 260 334 268 342 4.91e-16 81.3
MsaG045178 MtrunA17_Chr6g0485431 47.368 76 38 2 246 320 141 215 1.17e-15 79.0
MsaG045178 MtrunA17_Chr7g0238921 58.621 58 23 1 259 316 124 180 4.01e-15 76.6
MsaG045178 MtrunA17_Chr8g0348311 47.887 71 37 0 259 329 155 225 6.95e-15 76.3
MsaG045178 MtrunA17_Chr4g0071521 50.000 64 30 1 254 317 242 303 7.57e-15 75.9
MsaG045178 MtrunA17_Chr7g0248691 39.560 91 52 1 242 329 134 224 1.27e-14 75.5
MsaG045178 MtrunA17_Chr8g0387501 42.466 73 42 0 257 329 127 199 5.42e-14 72.8
MsaG045178 MtrunA17_Chr5g0432071 42.308 78 43 2 259 336 104 179 5.70e-14 71.2
MsaG045178 MtrunA17_Chr1g0202461 35.455 110 66 2 225 329 222 331 1.13e-13 73.2
MsaG045178 MtrunA17_Chr1g0189391 38.298 94 54 3 227 317 63 155 1.31e-12 69.3
MsaG045178 MtrunA17_Chr8g0387981 35.172 145 73 5 179 314 57 189 1.36e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045178 AT5G15130.1 42.799 493 198 18 63 527 28 464 1.44e-88 285
MsaG045178 AT5G15130.2 42.887 478 189 18 78 527 1 422 7.83e-85 275
MsaG045178 AT1G18860.1 38.421 570 235 19 7 527 8 510 1.95e-79 261
MsaG045178 AT1G69810.1 38.788 330 168 9 68 394 23 321 3.01e-57 198
MsaG045178 AT1G68150.1 81.633 98 18 0 243 340 219 316 4.10e-53 186
MsaG045178 AT4G04450.2 37.921 356 152 12 67 394 8 322 8.65e-53 187
MsaG045178 AT4G04450.1 38.202 356 151 12 67 394 102 416 5.79e-52 187
MsaG045178 AT1G62300.1 42.808 292 120 7 243 531 296 543 3.83e-46 171
MsaG045178 AT1G69810.2 38.776 294 146 9 104 394 1 263 4.45e-46 166
MsaG045178 AT4G01720.1 77.778 81 18 0 239 319 219 299 2.28e-39 151
MsaG045178 AT4G31800.2 54.321 81 37 0 257 337 109 189 2.65e-26 108
MsaG045178 AT4G31800.1 54.321 81 37 0 257 337 174 254 4.61e-26 109
MsaG045178 AT2G25000.2 65.079 63 22 0 257 319 101 163 8.36e-25 103
MsaG045178 AT2G25000.4 65.079 63 22 0 257 319 101 163 8.36e-25 103
MsaG045178 AT2G25000.3 65.079 63 22 0 257 319 123 185 1.54e-24 103
MsaG045178 AT2G25000.1 65.079 63 22 0 257 319 144 206 1.75e-24 103
MsaG045178 AT1G80840.1 61.538 65 25 0 255 319 142 206 2.71e-24 103
MsaG045178 AT2G38470.1 47.778 90 46 1 242 331 345 433 1.60e-23 105
MsaG045178 AT2G38470.1 51.667 60 27 2 260 319 185 242 9.57e-13 71.2
MsaG045178 AT1G29860.1 36.364 154 85 4 243 396 120 260 2.06e-23 100
MsaG045178 AT5G56270.1 54.545 77 34 1 243 319 471 546 4.09e-23 104
MsaG045178 AT5G56270.1 51.667 60 27 2 260 319 274 331 9.20e-12 68.6
MsaG045178 AT4G26640.5 44.898 98 53 1 227 324 173 269 5.24e-23 102
MsaG045178 AT4G26640.5 39.773 88 49 3 251 336 25 110 1.12e-11 67.4
MsaG045178 AT4G26640.6 44.898 98 53 1 227 324 173 269 5.24e-23 102
MsaG045178 AT4G26640.6 39.773 88 49 3 251 336 25 110 1.12e-11 67.4
MsaG045178 AT4G26640.4 44.898 98 53 1 227 324 173 269 5.24e-23 102
MsaG045178 AT4G26640.4 39.773 88 49 3 251 336 25 110 1.12e-11 67.4
MsaG045178 AT4G26640.3 44.898 98 53 1 227 324 173 269 5.24e-23 102
MsaG045178 AT4G26640.3 39.773 88 49 3 251 336 25 110 1.12e-11 67.4
MsaG045178 AT4G26640.7 44.898 98 53 1 227 324 309 405 1.24e-22 102
MsaG045178 AT4G26640.7 46.479 71 34 3 251 319 161 229 1.70e-11 67.4
MsaG045178 AT4G26640.1 44.898 98 53 1 227 324 277 373 1.33e-22 102
MsaG045178 AT4G26640.1 46.479 71 34 3 251 319 129 197 1.78e-11 67.4
MsaG045178 AT4G26640.2 44.898 98 53 1 227 324 349 445 2.00e-22 102
MsaG045178 AT4G26640.2 46.479 71 34 3 251 319 201 269 2.03e-11 67.4
MsaG045178 AT2G03340.1 41.667 108 62 1 217 324 373 479 3.01e-22 101
MsaG045178 AT2G03340.1 50.746 67 30 3 253 319 245 308 3.95e-14 75.9
MsaG045178 AT4G30935.1 44.954 109 57 2 212 320 286 391 6.34e-22 99.8
MsaG045178 AT1G13960.1 44.792 96 46 2 230 319 374 468 8.56e-22 99.8
MsaG045178 AT1G13960.1 49.254 67 31 3 253 319 224 287 6.27e-13 72.0
MsaG045178 AT1G13960.2 44.792 96 46 2 230 319 221 315 1.39e-21 97.4
MsaG045178 AT1G13960.2 49.254 67 31 3 253 319 71 134 7.17e-13 70.9
MsaG045178 AT5G07100.2 51.282 78 37 1 242 319 124 200 1.48e-21 94.0
MsaG045178 AT5G07100.2 42.105 76 43 1 257 332 22 96 4.10e-12 66.6
MsaG045178 AT5G07100.4 51.282 78 37 1 242 319 124 200 1.48e-21 94.0
MsaG045178 AT5G07100.4 42.105 76 43 1 257 332 22 96 4.10e-12 66.6
MsaG045178 AT5G07100.5 51.282 78 37 1 242 319 124 200 1.48e-21 94.0
MsaG045178 AT5G07100.5 42.105 76 43 1 257 332 22 96 4.10e-12 66.6
MsaG045178 AT5G07100.3 51.282 78 37 1 242 319 124 200 1.48e-21 94.0
MsaG045178 AT5G07100.3 42.105 76 43 1 257 332 22 96 4.10e-12 66.6
MsaG045178 AT5G07100.1 51.282 78 37 1 242 319 217 293 2.01e-21 95.9
MsaG045178 AT5G07100.1 42.105 76 43 1 257 332 115 189 8.82e-12 67.0
MsaG045178 AT2G04880.2 52.000 75 35 1 245 319 269 342 3.11e-21 97.4
MsaG045178 AT2G04880.2 41.237 97 52 4 257 353 109 200 8.81e-13 71.2
MsaG045178 AT5G26170.1 50.685 73 35 1 245 317 99 170 3.22e-21 91.7
MsaG045178 AT5G41570.1 43.299 97 54 1 220 316 59 154 3.27e-21 92.0
MsaG045178 AT4G26440.1 35.028 177 98 5 210 386 326 485 3.84e-21 98.2
MsaG045178 AT4G26440.1 49.254 67 31 3 253 319 173 236 1.68e-11 67.4
MsaG045178 AT2G04880.1 52.000 75 35 1 245 319 293 366 5.09e-21 97.1
MsaG045178 AT2G04880.1 45.588 68 35 2 257 324 109 174 1.74e-12 70.5
MsaG045178 AT3G01080.2 51.351 74 35 1 246 319 331 403 2.23e-20 94.7
MsaG045178 AT3G01080.1 51.351 74 35 1 246 319 293 365 2.35e-20 94.4
MsaG045178 AT2G30250.1 42.857 98 52 2 225 319 291 387 2.57e-20 94.0
MsaG045178 AT2G44745.1 44.186 86 47 1 231 316 117 201 2.87e-20 90.1
MsaG045178 AT2G47260.1 43.810 105 54 2 239 339 154 257 2.90e-20 92.8
MsaG045178 AT5G49520.1 46.835 79 41 1 243 321 205 282 3.37e-20 93.6
MsaG045178 AT4G18170.1 36.090 133 78 3 222 354 141 266 6.17e-20 91.7
MsaG045178 AT5G46350.1 46.591 88 45 2 246 332 170 256 9.77e-20 91.3
MsaG045178 AT1G64000.1 52.113 71 33 1 246 316 101 170 3.44e-19 86.7
MsaG045178 AT5G28650.1 62.712 59 22 0 264 322 267 325 5.45e-19 89.0
MsaG045178 AT1G69310.4 42.574 101 51 2 216 316 110 203 6.91e-19 87.8
MsaG045178 AT1G69310.2 42.574 101 51 2 216 316 110 203 6.91e-19 87.8
MsaG045178 AT1G69310.1 42.574 101 51 2 216 316 110 203 6.91e-19 87.8
MsaG045178 AT5G13080.1 47.945 73 37 1 246 318 54 125 8.05e-19 84.0
MsaG045178 AT5G64810.1 45.000 80 43 1 237 316 88 166 1.15e-18 85.1
MsaG045178 AT1G55600.1 44.872 78 42 1 242 319 290 366 1.44e-18 89.4
MsaG045178 AT4G24240.1 52.778 72 32 1 254 325 278 347 1.48e-18 88.2
MsaG045178 AT3G04670.1 59.322 59 24 0 264 322 267 325 1.94e-18 87.4
MsaG045178 AT2G23320.1 59.322 59 24 0 260 318 241 299 2.78e-18 86.7
MsaG045178 AT1G29280.1 47.297 74 39 0 250 323 65 138 5.03e-18 84.7
MsaG045178 AT2G46130.1 51.351 74 35 1 243 316 14 86 6.54e-18 80.1
MsaG045178 AT4G39410.1 40.476 84 49 1 234 317 198 280 8.14e-18 85.1
MsaG045178 AT3G62340.1 48.193 83 42 1 238 320 97 178 8.15e-18 84.7
MsaG045178 AT2G30590.1 46.988 83 36 1 242 316 288 370 2.58e-17 84.7
MsaG045178 AT2G37260.2 56.923 65 27 1 257 321 265 328 3.21e-17 84.0
MsaG045178 AT2G37260.2 44.444 99 52 3 260 358 84 179 7.41e-17 82.8
MsaG045178 AT2G37260.3 56.923 65 27 1 257 321 267 330 3.47e-17 84.0
MsaG045178 AT2G37260.3 44.444 99 52 3 260 358 86 181 8.07e-17 82.8
MsaG045178 AT2G37260.1 56.923 65 27 1 257 321 347 410 6.87e-17 84.0
MsaG045178 AT2G37260.1 44.444 99 52 3 260 358 166 261 1.81e-16 82.4
MsaG045178 AT3G01970.1 47.297 74 38 1 245 318 51 123 9.79e-17 78.2
MsaG045178 AT4G31550.1 35.088 171 81 7 169 316 140 303 1.83e-16 81.3
MsaG045178 AT4G31550.2 46.154 78 35 2 239 316 232 302 2.89e-16 80.9
MsaG045178 AT2G24570.1 39.806 103 51 3 239 341 230 321 4.35e-16 80.1
MsaG045178 AT4G23550.1 44.156 77 43 0 258 334 139 215 2.83e-15 77.4
MsaG045178 AT1G30650.1 49.254 67 34 0 260 326 218 284 3.43e-15 78.6
MsaG045178 AT3G58710.1 52.308 65 31 0 259 323 71 135 4.17e-15 76.3
MsaG045178 AT3G58710.2 52.308 65 31 0 259 323 70 134 4.31e-15 76.3
MsaG045178 AT4G01250.1 46.479 71 38 0 259 329 128 198 4.71e-15 76.6
MsaG045178 AT2G34830.1 49.254 67 34 0 260 326 216 282 8.84e-15 77.4
MsaG045178 AT2G34830.2 49.254 67 34 0 260 326 225 291 1.09e-14 77.0
MsaG045178 AT4G31800.3 67.500 40 13 0 257 296 174 213 1.32e-14 74.3
MsaG045178 AT5G52830.1 48.571 70 36 0 260 329 166 235 2.64e-14 75.1
MsaG045178 AT1G69310.3 39.604 101 50 3 216 316 110 199 5.36e-14 73.2
MsaG045178 AT2G21900.1 45.161 62 33 1 258 319 108 168 7.72e-14 71.2
MsaG045178 AT2G46130.2 47.297 74 34 2 243 316 14 82 5.02e-13 66.2
MsaG045178 AT5G43290.1 51.724 58 27 1 259 316 114 170 5.19e-13 70.1
MsaG045178 AT5G24110.1 46.575 73 36 2 244 314 99 170 1.77e-12 68.9
MsaG045178 AT5G45050.4 34.483 116 74 1 211 326 995 1108 7.71e-12 69.3
MsaG045178 AT5G45050.2 35.780 109 68 1 211 319 1106 1212 7.75e-12 69.3
MsaG045178 AT5G45050.1 35.780 109 68 1 211 319 1134 1240 7.86e-12 69.3

Find 118 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTGGACTTGGAATATT+TGG 0.129902 8:-69643912 None:intergenic
CCATATATGGAGTGCAATTC+TGG 0.170613 8:+69641294 None:intergenic
GCAGCACTTTCTTCACTTAT+TGG 0.177197 8:+69645307 MsaT045178.1:CDS
CAATGCATGGGATTTGATTC+AGG 0.243163 8:-69645043 None:intergenic
ATAAATAATCACCAATTAAT+TGG 0.256352 8:-69645593 None:intergenic
TCATCAAGTTCTAAAGTAAA+TGG 0.266329 8:+69645430 MsaT045178.1:CDS
GGAAATGCAATTTAACAATA+TGG 0.281542 8:+69643632 MsaT045178.1:CDS
ATTCTGCATTTATTTCTTCA+TGG 0.288137 8:-69643701 None:intergenic
TTGTTCCTATTATTACTATA+TGG 0.291383 8:-69645103 None:intergenic
GAAATGGGCTGAGATGTTTC+AGG 0.315609 8:+69645387 MsaT045178.1:CDS
CATTTCCAGTGCTCGGTATT+CGG 0.345477 8:-69643616 None:intergenic
CAAACCCTAGCTTTCTTTGC+AGG 0.346131 8:-69644047 None:intergenic
ACAACTTCATCATCCTTTGT+TGG 0.363180 8:-69643951 None:intergenic
ATCAACCATATAGTAATAAT+AGG 0.373148 8:+69645098 MsaT045178.1:CDS
TGAGACAACAAAAGCTCAAA+TGG 0.387526 8:+69643539 MsaT045178.1:CDS
GCTAAAGCTGATTGAAAAGT+TGG 0.388661 8:-69645283 None:intergenic
GTCTTATTGAGTGTCTTACT+TGG 0.404266 8:-69643987 None:intergenic
GGATGCCAATGGAGAAAGTA+TGG 0.406659 8:+69644381 MsaT045178.1:CDS
CTTATTGGAAACACTAATCA+AGG 0.409882 8:+69645322 MsaT045178.1:CDS
CCATTCTGTGTATTGTTCAC+TGG 0.411705 8:-69645484 None:intergenic
CAAACCAACAACAATCTTCA+TGG 0.413700 8:+69644803 MsaT045178.1:CDS
TTTGTCCATACTTTCTCCAT+TGG 0.414359 8:-69644386 None:intergenic
ATCATGTCCGGTTAGAAAAC+AGG 0.417854 8:+69644464 MsaT045178.1:CDS
GGAAGAGTTGTCACTTGGAT+TGG 0.421095 8:+69643842 MsaT045178.1:CDS
CATCTGGTAGATAAAAGTTT+AGG 0.422150 8:-69644826 None:intergenic
AACCTAAGCACTTTAAACTT+TGG 0.427632 8:+69645130 MsaT045178.1:CDS
GATGATGTTGAAGAATTTGA+TGG 0.429432 8:-69644938 None:intergenic
CACAGTTGCACCATCATGTC+CGG 0.429469 8:+69644452 MsaT045178.1:CDS
AAGAAGGGTATCTTGGTAGT+TGG 0.437752 8:-69645000 None:intergenic
ATTTGAGTCAGAAAATGAAA+TGG 0.439076 8:+69645371 MsaT045178.1:CDS
TTTGAGTAGTAGCATAATTG+AGG 0.451637 8:-69645075 None:intergenic
TTGTTGTTGGTTTGTGCTGA+TGG 0.452268 8:-69644794 None:intergenic
GGTTCATCAACCTCCAACTC+TGG 0.460772 8:+69644764 MsaT045178.1:CDS
CATATATGGAGTGCAATTCT+GGG 0.463692 8:+69641295 None:intergenic
TTGGGCGAGGATATTGTGCT+TGG 0.464830 8:-69643266 None:intergenic
GCCGCATCGATGCTATTATC+AGG 0.467031 8:+69644743 MsaT045178.1:CDS
ATGATAGTGCAGGGTTGGAT+AGG 0.467366 8:-69644871 None:intergenic
AGAGTGGAAGAAGGGTATCT+TGG 0.467928 8:-69645007 None:intergenic
AAACTCCTGCAAAGAAAGCT+AGG 0.468281 8:+69644042 MsaT045178.1:CDS
GATCTTTCAGTATTAGCAAT+GGG 0.469342 8:-69643230 None:intergenic
TTTGAGTCAGAAAATGAAAT+GGG 0.477793 8:+69645372 MsaT045178.1:CDS
CGATGCGGCCGCGGAAGTTG+TGG 0.478396 8:-69644729 None:intergenic
GATCTTGTTTCCCTCAGCCT+TGG 0.480636 8:+69643726 MsaT045178.1:CDS
GTGAGGTCAAGAGTGATAGT+TGG 0.491969 8:-69644908 None:intergenic
AACAAGGAAGAGTTGTCACT+TGG 0.493048 8:+69643837 MsaT045178.1:CDS
TTCTCAAAACAGATGAATGA+TGG 0.497069 8:+69644360 MsaT045178.1:intron
AAATTTGAAACATCAAAATC+AGG 0.497604 8:+69643870 MsaT045178.1:CDS
TACCAAAGTTTAAAGTGCTT+AGG 0.497709 8:-69645132 None:intergenic
TAAATTGCATTTCCAGTGCT+CGG 0.498132 8:-69643623 None:intergenic
TCAGAACTATTCACTGGACT+TGG 0.498507 8:-69643921 None:intergenic
CTAAAGCTAAGAGTGGAAGA+AGG 0.503500 8:-69645016 None:intergenic
CGATAGTAAGCTCGAGGGCA+AGG 0.506033 8:-69644426 None:intergenic
TTCAAGTTTCTTGAACAAAA+CGG 0.507107 8:+69645459 MsaT045178.1:CDS
AGTTCTAAAGTAAATGGTTG+TGG 0.510261 8:+69645436 MsaT045178.1:CDS
TTTACTTTAGAACTTGATGA+AGG 0.512151 8:-69645427 None:intergenic
TAGGCCATGAAGATTGTTGT+TGG 0.513416 8:-69644807 None:intergenic
GAGTGCAATTCTGGGAGATC+TGG 0.518269 8:+69641303 MsaT045178.1:CDS
TTATTGAGTGTCTTACTTGG+TGG 0.519260 8:-69643984 None:intergenic
TTGAGATGATAGTGCAGGGT+TGG 0.519761 8:-69644876 None:intergenic
CTGAATCAAATCCCATGCAT+TGG 0.522577 8:+69645044 MsaT045178.1:CDS
GCCATGGCTGTTGCTGAAAG+TGG 0.522889 8:-69644704 None:intergenic
CGCGGAAGTTGTGGAAGCCA+TGG 0.523187 8:-69644720 None:intergenic
AAAAGTGCTTCTGCATCTCC+TGG 0.524615 8:+69645550 MsaT045178.1:CDS
AAGAGAAGTGAGCATAGTAC+TGG 0.527731 8:+69641354 MsaT045178.1:CDS
AATTTGATGGGTTTGAAGTG+AGG 0.528347 8:-69644925 None:intergenic
GGTGCAGAAAATGGCAAAGA+AGG 0.529278 8:-69645526 None:intergenic
CTTGAATTGAATCTAACAAA+GGG 0.532209 8:-69644962 None:intergenic
TAGGAACTCTTCCAAGGCTG+AGG 0.532985 8:-69643737 None:intergenic
ACAACTTCAGAACTATTCAC+TGG 0.533241 8:-69643927 None:intergenic
TCCACTTTCAGCAACAGCCA+TGG 0.540367 8:+69644703 MsaT045178.1:CDS
TCAAACATTTCACAACATGT+AGG 0.542220 8:+69645211 MsaT045178.1:CDS
TTCTGAAGTTGTTCCAACAA+AGG 0.546847 8:+69643938 MsaT045178.1:CDS
GGGAATCAAAATCAAAGTGC+AGG 0.547602 8:+69645343 MsaT045178.1:CDS
GAGACAACAAAAGCTCAAAT+GGG 0.551564 8:+69643540 MsaT045178.1:CDS
CCAGTGAACAATACACAGAA+TGG 0.554001 8:+69645484 MsaT045178.1:CDS
ACTGAAAGATCAATTGAGGC+TGG 0.554571 8:+69643242 MsaT045178.1:CDS
AAATCAGGTAGCACAACTGA+AGG 0.555466 8:+69643885 MsaT045178.1:CDS
TGATCTTTCAGTATTAGCAA+TGG 0.556800 8:-69643231 None:intergenic
GAGGAAGCTATTCCAATGCA+TGG 0.564369 8:-69645056 None:intergenic
ATGATGAAGTTGTAGAGACT+TGG 0.570390 8:+69643961 MsaT045178.1:CDS
AGGAAGCTATTCCAATGCAT+GGG 0.575316 8:-69645055 None:intergenic
TGGCTGTTGCTGAAAGTGGA+AGG 0.576476 8:-69644700 None:intergenic
AATGACCGAATACCGAGCAC+TGG 0.578776 8:+69643611 MsaT045178.1:CDS
AGGAGAACTAAAGCTAAGAG+TGG 0.580510 8:-69645023 None:intergenic
TGATGGAGATAGTGAACTCA+AGG 0.581059 8:+69641326 MsaT045178.1:CDS
TAAAGCTAAGAGTGGAAGAA+GGG 0.581456 8:-69645015 None:intergenic
ATGATGTTGAAGAATTTGAT+GGG 0.583175 8:-69644937 None:intergenic
TTGCTGAAAGTGGAAGGCTG+TGG 0.584244 8:-69644694 None:intergenic
TGTTACTAGGAACTCTTCCA+AGG 0.584869 8:-69643743 None:intergenic
TAATACTGAAAGATCAATTG+AGG 0.589489 8:+69643238 MsaT045178.1:CDS
AACAAAAGCTCAAATGGGTG+AGG 0.591231 8:+69643545 MsaT045178.1:CDS
TAATAGCATCGATGCGGCCG+CGG 0.591990 8:-69644738 None:intergenic
CATGGCTTCCACAACTTCCG+CGG 0.593483 8:+69644721 MsaT045178.1:CDS
AACTCCTGCAAAGAAAGCTA+GGG 0.594594 8:+69644043 MsaT045178.1:CDS
GAATGTTGAGATGATAGTGC+AGG 0.594784 8:-69644881 None:intergenic
GCTTGAATTGAATCTAACAA+AGG 0.596663 8:-69644963 None:intergenic
TGGCATTGAACCAGAGTTGG+AGG 0.598647 8:-69644774 None:intergenic
AGGAACTCTTCCAAGGCTGA+GGG 0.600114 8:-69643736 None:intergenic
TCAGGTACAAAGATGTGTTG+AGG 0.608124 8:+69644631 MsaT045178.1:intron
TAAGACAATGAGAGATGCTG+AGG 0.621356 8:+69644004 MsaT045178.1:CDS
AGGTGAGAAAAGAAAATCAA+CGG 0.624151 8:+69643565 MsaT045178.1:CDS
TGATGGCATTGAACCAGAGT+TGG 0.628021 8:-69644777 None:intergenic
TTCTAATCACAACATACGAA+GGG 0.629312 8:+69644663 MsaT045178.1:CDS
TAGACAAGTATTTACCGTTG+GGG 0.632603 8:-69644090 None:intergenic
GTGCAACGATAGTAAGCTCG+AGG 0.633302 8:-69644432 None:intergenic
TTATTGGAAACACTAATCAA+GGG 0.641347 8:+69645323 MsaT045178.1:CDS
ATTCTAATCACAACATACGA+AGG 0.641504 8:+69644662 MsaT045178.1:CDS
AATCAGGTAGCACAACTGAA+GGG 0.644837 8:+69643886 MsaT045178.1:CDS
AGATGAATGATGGATGCCAA+TGG 0.647635 8:+69644370 MsaT045178.1:intron
AGCAAGATGTGACACCCCAA+CGG 0.667652 8:+69644076 MsaT045178.1:CDS
CTTTGGTAGACAAAACACAA+TGG 0.673621 8:+69645147 MsaT045178.1:CDS
TTGTCACTATTATCACCACC+AGG 0.684962 8:-69645568 None:intergenic
TGCAACGATAGTAAGCTCGA+GGG 0.697067 8:-69644431 None:intergenic
AGTGCTTCTGCATCTCCTGG+TGG 0.699273 8:+69645553 MsaT045178.1:CDS
ACCTGATAATAGCATCGATG+CGG 0.704481 8:-69644744 None:intergenic
TAATGATGAAGAATTCAACA+AGG 0.709467 8:+69643821 MsaT045178.1:CDS
AATGTTGAGATGATAGTGCA+GGG 0.717220 8:-69644880 None:intergenic
AATTCTGGGAGATCTGGTGA+TGG 0.720675 8:+69641309 MsaT045178.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 69641300 69645600 69641300 ID=MsaG045178
Chr8 mRNA 69641300 69645600 69641300 ID=MsaT045178.1;Parent=MsaG045178
Chr8 exon 69641300 69641422 69641300 ID=MsaT045178.1.exon1;Parent=MsaT045178.1
Chr8 CDS 69641300 69641422 69641300 ID=cds.MsaT045178.1;Parent=MsaT045178.1
Chr8 exon 69643224 69643298 69643224 ID=MsaT045178.1.exon2;Parent=MsaT045178.1
Chr8 CDS 69643224 69643298 69643224 ID=cds.MsaT045178.1;Parent=MsaT045178.1
Chr8 exon 69643525 69644097 69643525 ID=MsaT045178.1.exon3;Parent=MsaT045178.1
Chr8 CDS 69643525 69644097 69643525 ID=cds.MsaT045178.1;Parent=MsaT045178.1
Chr8 exon 69644372 69644485 69644372 ID=MsaT045178.1.exon4;Parent=MsaT045178.1
Chr8 CDS 69644372 69644485 69644372 ID=cds.MsaT045178.1;Parent=MsaT045178.1
Chr8 exon 69644635 69645600 69644635 ID=MsaT045178.1.exon5;Parent=MsaT045178.1
Chr8 CDS 69644635 69645600 69644635 ID=cds.MsaT045178.1;Parent=MsaT045178.1
Gene Sequence

>MsaT045178.1

ATGGAGTGCAATTCTGGGAGATCTGGTGATGGAGATAGTGAACTCAAGGAAGATAAGAGAAGTGAGCATAGTACTGGTGATGATCATGAAAGTCATCATCAAAAAAAGGAGACTATTGTTGAGGAAGCACCCATTGCTAATACTGAAAGATCAATTGAGGCTGGTCCAAGCACAATATCCTCGCCCAAAAATGATAAGGTTGATGATCAGCTTGAGACAACAAAAGCTCAAATGGGTGAGGTGAGAAAAGAAAATCAACGGTTAAAGATGTGCTTGAACAAAATAATGACCGAATACCGAGCACTGGAAATGCAATTTAACAATATGGTTAAGCAAGAAACAAAAAAGAGCAATGTTGATCACAATGATAATAACCATGAAGAAATAAATGCAGAATCAGATCTTGTTTCCCTCAGCCTTGGAAGAGTTCCTAGTAACAATATTCCAAAAAATGATCAAGAGAAAGTTAACAAAGTGTCTAAACTAGCATTGAATAATGATGAAGAATTCAACAAGGAAGAGTTGTCACTTGGATTGGAATGTAAATTTGAAACATCAAAATCAGGTAGCACAACTGAAGGGTTACCAAATATTCCAAGTCCAGTGAATAGTTCTGAAGTTGTTCCAACAAAGGATGATGAAGTTGTAGAGACTTGGCCACCAAGTAAGACACTCAATAAGACAATGAGAGATGCTGAGGAAGAAGTTGCTCAACAAACTCCTGCAAAGAAAGCTAGGGTTTGTGTTAGAGCAAGATGTGACACCCCAACGATGAATGATGGATGCCAATGGAGAAAGTATGGACAAAAAATTGCAAAGGGAAATCCTTGCCCTCGAGCTTACTATCGTTGCACAGTTGCACCATCATGTCCGGTTAGAAAACAGGTACAAAGATGTGTTGAGGACATGTCTATTCTAATCACAACATACGAAGGGACACACAACCACAGCCTTCCACTTTCAGCAACAGCCATGGCTTCCACAACTTCCGCGGCCGCATCGATGCTATTATCAGGTTCATCAACCTCCAACTCTGGTTCAATGCCATCAGCACAAACCAACAACAATCTTCATGGCCTAAACTTTTATCTACCAGATGGTTCAAAATCAAATCAATTATACCTATCCAACCCTGCACTATCATCTCAACATTCACATCCAACTATCACTCTTGACCTCACTTCAAACCCATCAAATTCTTCAACATCATCGCCCTTTGTTAGATTCAATTCAAGCTACAACAACAATAACCAACTACCAAGATACCCTTCTTCCACTCTTAGCTTTAGTTCTCCTGAATCAAATCCCATGCATTGGAATAGCTTCCTCAATTATGCTACTACTCAAAATCAACCATATAGTAATAATAGGAACAATAATAACCTAAGCACTTTAAACTTTGGTAGACAAAACACAATGGAAAGTATATACCAAACCTACATGCAAAAAAACAACAATAGTTCAAACATTTCACAACATGTAGGTTTACAAGATAGTACTATATCTGCAGCAACAAAAGCTATCACAGCAGATCCAACTTTTCAATCAGCTTTAGCTGCAGCACTTTCTTCACTTATTGGAAACACTAATCAAGGGAATCAAAATCAAAGTGCAGGAGAAAATTTGAGTCAGAAAATGAAATGGGCTGAGATGTTTCAGGTTTCTTCTACTTCATTGCCTTCATCAAGTTCTAAAGTAAATGGTTGTGGTTCAAGTTTCTTGAACAAAACGGCACCAGTGAACAATACACAGAATGGAAGTTTGATGTTGTTATCTCCTTCTTTGCCATTTTCTGCACCTAAAAGTGCTTCTGCATCTCCTGGTGGTGATAATAGTGACAATACCAATTAA
Protein sequence

>MsaT045178.1

MECNSGRSGDGDSELKEDKRSEHSTGDDHESHHQKKETIVEEAPIANTERSIEAGPSTISSPKNDKVDDQLETTKAQMGEVRKENQRLKMCLNKIMTEYRALEMQFNNMVKQETKKSNVDHNDNNHEEINAESDLVSLSLGRVPSNNIPKNDQEKVNKVSKLALNNDEEFNKEELSLGLECKFETSKSGSTTEGLPNIPSPVNSSEVVPTKDDEVVETWPPSKTLNKTMRDAEEEVAQQTPAKKARVCVRARCDTPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTVAPSCPVRKQVQRCVEDMSILITTYEGTHNHSLPLSATAMASTTSAAASMLLSGSSTSNSGSMPSAQTNNNLHGLNFYLPDGSKSNQLYLSNPALSSQHSHPTITLDLTSNPSNSSTSSPFVRFNSSYNNNNQLPRYPSSTLSFSSPESNPMHWNSFLNYATTQNQPYSNNRNNNNLSTLNFGRQNTMESIYQTYMQKNNNSSNISQHVGLQDSTISAATKAITADPTFQSALAAALSSLIGNTNQGNQNQSAGENLSQKMKWAEMFQVSSTSLPSSSSKVNGCGSSFLNKTAPVNNTQNGSLMLLSPSLPFSAPKSASASPGGDNSDNTN*