AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045212


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045212 MtrunA17_Chr4g0060761 72.941 255 28 5 30 246 22 273 4.23e-119 340
MsaG045212 MtrunA17_Chr3g0092141 53.757 173 67 4 82 245 5 173 9.44e-53 169
MsaG045212 MtrunA17_Chr3g0127001 53.704 108 41 2 133 240 159 257 3.33e-30 113
MsaG045212 MtrunA17_Chr1g0182631 48.276 116 51 1 125 240 124 230 4.35e-28 107
MsaG045212 MtrunA17_Chr5g0406901 35.802 162 89 3 85 242 169 319 3.36e-23 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045212 AT1G18790.1 65.217 115 39 1 125 238 125 239 3.04e-47 158
MsaG045212 AT1G74480.1 40.928 237 120 6 16 234 8 242 2.84e-44 151
MsaG045212 AT5G66990.1 58.678 121 48 2 122 240 153 273 1.95e-41 143
MsaG045212 AT5G53040.1 55.856 111 40 1 128 238 148 249 8.52e-34 122
MsaG045212 AT4G35590.2 34.667 150 89 3 92 235 157 303 4.02e-19 85.1
MsaG045212 AT4G35590.1 34.667 150 89 3 92 235 207 353 7.00e-19 85.1
MsaG045212 AT5G16100.2 41.176 85 48 1 125 207 351 435 1.09e-13 70.5

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTGCAGCTTGTGTTAT+TGG 0.135392 8:-70069858 None:intergenic
TGACTTACATGTTAGGAAAT+TGG 0.240258 8:-70069241 None:intergenic
GTTGATGGATATCGAGAAAT+TGG 0.245834 8:+70070151 MsaT045212.1:CDS
AATTGGCGGTTATATGAAAA+TGG 0.251270 8:-70069224 None:intergenic
TGATCTTGCAATTGTATATC+TGG 0.302877 8:-70070203 None:intergenic
AACCTTGATCAATAATATTC+AGG 0.325902 8:+70069968 MsaT045212.1:CDS
ACAATGTTGTCTAAGCCTTT+TGG 0.326924 8:-70070228 None:intergenic
TTACAAGAAAAGAAAGCTTA+TGG 0.338403 8:+70070262 MsaT045212.1:CDS
CTGCAGACGACTATGAAATT+TGG 0.367816 8:+70069646 MsaT045212.1:intron
ACTTAGTGTTGAAAAGTTGA+TGG 0.379342 8:+70070136 MsaT045212.1:CDS
TATTATTGATCAAGGTTTGC+AGG 0.383508 8:-70069962 None:intergenic
ACCCATACACAATGATGGTT+TGG 0.385554 8:+70069386 MsaT045212.1:CDS
TTCCAAACCATCATTGTGTA+TGG 0.398088 8:-70069388 None:intergenic
ACACAATGATGGTTTGGAAC+TGG 0.400074 8:+70069392 MsaT045212.1:CDS
AGTTTCATGACTTACATGTT+AGG 0.403480 8:-70069248 None:intergenic
TACCTGAATATTATTGATCA+AGG 0.420609 8:-70069970 None:intergenic
TATGGTATGGAGAAAGGATT+TGG 0.421835 8:+70069669 MsaT045212.1:CDS
TGATGATAACCTTCCAGCCT+TGG 0.426088 8:-70069477 None:intergenic
TGAAATTAACAAAGAGGTTG+TGG 0.431311 8:+70069749 MsaT045212.1:CDS
GGCATTGGTTCAATGTATAG+AGG 0.437542 8:-70069456 None:intergenic
ATTTGGTATGGTATGGAGAA+AGG 0.442668 8:+70069663 MsaT045212.1:CDS
TGTATAGAGGGTGATGATGA+GGG 0.456423 8:-70069443 None:intergenic
AGAGTATGCCACTTACTTGT+TGG 0.466970 8:+70069174 MsaT045212.1:CDS
CATTGAACCAATGCCAAGGC+TGG 0.480155 8:+70069464 MsaT045212.1:CDS
CTATGAAATTTGGTATGGTA+TGG 0.481213 8:+70069656 MsaT045212.1:CDS
AGAGGAAATGAAGCTGGTGG+AGG 0.485504 8:+70070175 MsaT045212.1:CDS
GTTGCAGGAGCTAGGGAAAG+AGG 0.496134 8:+70070106 MsaT045212.1:intron
TGGATTTGGTTGCAGGAGCT+AGG 0.503102 8:+70070098 MsaT045212.1:intron
GGAGTGGAGAGGATTAGTGA+TGG 0.512454 8:+70069786 MsaT045212.1:CDS
GGATTTGGTTGCAGGAGCTA+GGG 0.512821 8:+70070099 MsaT045212.1:intron
GGAGGTAAAGCTAGAAGGAG+TGG 0.514082 8:+70069770 MsaT045212.1:CDS
ATGTATAGAGGGTGATGATG+AGG 0.517675 8:-70069444 None:intergenic
GTTGTGGAGGTAAAGCTAGA+AGG 0.522008 8:+70069765 MsaT045212.1:CDS
TAAAAGCTCCAACAAGTAAG+TGG 0.525627 8:-70069182 None:intergenic
TAACCTTCCAGCCTTGGCAT+TGG 0.526844 8:-70069471 None:intergenic
TATTCACCCATACACAATGA+TGG 0.527857 8:+70069381 MsaT045212.1:CDS
GGATATCGAGAAATTGGAAG+AGG 0.537710 8:+70070157 MsaT045212.1:CDS
GACGACTATGAAATTTGGTA+TGG 0.546215 8:+70069651 MsaT045212.1:intron
CTTACATGTTAGGAAATTGG+CGG 0.547072 8:-70069238 None:intergenic
ATTGGAAGAGGAAATGAAGC+TGG 0.556172 8:+70070169 MsaT045212.1:CDS
AATTGCAAGATCATACCAAA+AGG 0.561321 8:+70070213 MsaT045212.1:CDS
TAAAGCTAGAAGGAGTGGAG+AGG 0.578999 8:+70069775 MsaT045212.1:CDS
GAACCAATGCCAAGGCTGGA+AGG 0.579018 8:+70069468 MsaT045212.1:CDS
GTATAGAGGGTGATGATGAG+GGG 0.582910 8:-70069442 None:intergenic
TCCAAACCATCATTGTGTAT+GGG 0.593952 8:-70069387 None:intergenic
GCATTGGTTCAATGTATAGA+GGG 0.595733 8:-70069455 None:intergenic
TGGAAATGAAATTAACAAAG+AGG 0.597270 8:+70069743 MsaT045212.1:CDS
AGCTGCAAGAGAACTGAATG+TGG 0.601755 8:+70069869 MsaT045212.1:CDS
GTAGGGAGTTGGGTATTCGT+AGG 0.615855 8:+70069916 MsaT045212.1:CDS
AATTAACAAAGAGGTTGTGG+AGG 0.641037 8:+70069752 MsaT045212.1:CDS
GCTGCAAGAGAACTGAATGT+GGG 0.643443 8:+70069870 MsaT045212.1:CDS
GGGAGTTGGGTATTCGTAGG+TGG 0.654663 8:+70069919 MsaT045212.1:CDS
GTAACACAAAGAATTTGTCA+AGG 0.655690 8:+70069808 MsaT045212.1:CDS
GGAAGAGGAAATGAAGCTGG+TGG 0.660598 8:+70070172 MsaT045212.1:CDS
CAGGAGCTAGGGAAAGAGGA+TGG 0.660990 8:+70070110 MsaT045212.1:intron
TATACATTGAACCAATGCCA+AGG 0.667387 8:+70069460 MsaT045212.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 70069170 70070304 70069170 ID=MsaG045212
Chr8 mRNA 70069170 70070304 70069170 ID=MsaT045212.1;Parent=MsaG045212
Chr8 exon 70069170 70069255 70069170 ID=MsaT045212.1.exon1;Parent=MsaT045212.1
Chr8 CDS 70069170 70069255 70069170 ID=cds.MsaT045212.1;Parent=MsaT045212.1
Chr8 exon 70069365 70069489 70069365 ID=MsaT045212.1.exon2;Parent=MsaT045212.1
Chr8 CDS 70069365 70069489 70069365 ID=cds.MsaT045212.1;Parent=MsaT045212.1
Chr8 exon 70069652 70069989 70069652 ID=MsaT045212.1.exon3;Parent=MsaT045212.1
Chr8 CDS 70069652 70069989 70069652 ID=cds.MsaT045212.1;Parent=MsaT045212.1
Chr8 exon 70070113 70070304 70070113 ID=MsaT045212.1.exon4;Parent=MsaT045212.1
Chr8 CDS 70070113 70070304 70070113 ID=cds.MsaT045212.1;Parent=MsaT045212.1
Gene Sequence

>MsaT045212.1

ATGGAGAGTATGCCACTTACTTGTTGGAGCTTTTACAATGACATAGATGAAAAGCCATTTTCATATAACCGCCAATTTCCTAACATTGGAATCATTAGTGAGTATTCACCCATACACAATGATGGTTTGGAACTGGATTTTGATTCTCTTATGAAGTGTAATTCCCCTCATCATCACCCTCTATACATTGAACCAATGCCAAGGCTGGAAGACGACTATGAAATTTGGTATGGTATGGAGAAAGGATTTGGTGACTTGAGCGAAATGATCGATGATTATGCAGCGTTTGATTCTGAAAAAAGTGGAAATGAAATTAACAAAGAGGTTGTGGAGGTAAAGCTAGAAGGAGTGGAGAGGATTAGTGATGGTAACACAAAGAATTTGTCAAGGAAGACCATATCTCAGTATTTTTATATGCCAATAACACAAGCTGCAAGAGAACTGAATGTGGGCCTCACACATTTGAAAAAACGGTGTAGGGAGTTGGGTATTCGTAGGTGGCCCCATAGAAAACTCATGAGCCTGCAAACCTTGATCAATAATATTCAGGAGCTAGGGAAAGAGGATGGTCTACTTAGTGTTGAAAAGTTGATGGATATCGAGAAATTGGAAGAGGAAATGAAGCTGGTGGAGGAGATGCCAGATATACAATTGCAAGATCATACCAAAAGGCTTAGACAACATTGTTTTAAGGCCAATTACAAGAAAAGAAAGCTTATGGATCAGCTGATCATGTTTTAA

Protein sequence

>MsaT045212.1

MESMPLTCWSFYNDIDEKPFSYNRQFPNIGIISEYSPIHNDGLELDFDSLMKCNSPHHHPLYIEPMPRLEDDYEIWYGMEKGFGDLSEMIDDYAAFDSEKSGNEINKEVVEVKLEGVERISDGNTKNLSRKTISQYFYMPITQAARELNVGLTHLKKRCRELGIRRWPHRKLMSLQTLINNIQELGKEDGLLSVEKLMDIEKLEEEMKLVEEMPDIQLQDHTKRLRQHCFKANYKKRKLMDQLIMF*