AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045220 MtrunA17_Chr4g0060841 97.191 356 10 0 1 356 1 356 0.0 716
MsaG045220 MtrunA17_Chr3g0134451 32.735 223 124 7 1 217 1 203 7.18e-29 114
MsaG045220 MtrunA17_Chr5g0417661 70.149 67 20 0 1 67 56 122 3.41e-25 99.0
MsaG045220 MtrunA17_Chr5g0417671 82.353 51 9 0 59 109 26 76 6.92e-22 88.6
MsaG045220 MtrunA17_Chr5g0425191 62.295 61 23 0 1 61 1 61 3.21e-21 87.4
MsaG045220 MtrunA17_Chr4g0044071 63.934 61 22 0 1 61 1 61 1.35e-20 85.9
MsaG045220 MtrunA17_Chr5g0427401 34.302 172 79 5 1 169 1 141 5.37e-18 82.0
MsaG045220 MtrunA17_Chr1g0159661 39.423 104 63 0 1 104 1 104 4.59e-17 78.2
MsaG045220 MtrunA17_Chr3g0125241 62.264 53 20 0 1 53 26 78 4.98e-16 75.9
MsaG045220 MtrunA17_Chr1g0173161 58.491 53 22 0 1 53 1 53 1.23e-15 71.6
MsaG045220 MtrunA17_Chr5g0412461 48.571 70 34 1 1 70 1 68 5.75e-15 73.6
MsaG045220 MtrunA17_Chr7g0246431 31.319 182 95 5 1 180 1 154 5.97e-15 73.2
MsaG045220 MtrunA17_Chr5g0412271 48.571 70 34 1 1 70 1 68 6.32e-15 73.2
MsaG045220 MtrunA17_Chr4g0020221 46.479 71 36 1 1 71 1 69 6.72e-15 73.9
MsaG045220 MtrunA17_Chr2g0279821 46.479 71 36 1 1 71 1 69 8.11e-15 73.2
MsaG045220 MtrunA17_Chr3g0142191 56.604 53 23 0 1 53 1 53 8.50e-15 73.2
MsaG045220 MtrunA17_Chr2g0308251 54.717 53 24 0 1 53 1 53 8.72e-15 69.3
MsaG045220 MtrunA17_Chr2g0284911 45.070 71 37 1 1 71 16 84 9.19e-15 73.6
MsaG045220 MtrunA17_Chr5g0420081 45.570 79 42 1 1 79 1 78 9.80e-15 72.8
MsaG045220 MtrunA17_Chr5g0427921 50.943 53 26 0 1 53 1 53 1.48e-14 67.8
MsaG045220 MtrunA17_Chr8g0366231 43.038 79 44 1 1 79 1 78 1.55e-14 72.4
MsaG045220 MtrunA17_Chr1g0164791 45.070 71 39 0 1 71 1 71 1.74e-14 72.0
MsaG045220 MtrunA17_Chr3g0097101 54.717 53 24 0 1 53 16 68 2.18e-14 72.0
MsaG045220 MtrunA17_Chr4g0061691 43.038 79 44 1 1 79 1 78 2.58e-14 71.6
MsaG045220 MtrunA17_Chr3g0077501 54.717 53 24 0 1 53 1 53 2.71e-14 71.2
MsaG045220 MtrunA17_Chr4g0049901 48.571 70 34 1 1 70 27 94 4.05e-14 71.2
MsaG045220 MtrunA17_Chr8g0380021 52.830 53 25 0 1 53 17 69 6.69e-14 70.9
MsaG045220 MtrunA17_Chr4g0049541 45.946 74 38 1 1 74 1 72 6.73e-14 70.5
MsaG045220 MtrunA17_Chr5g0411691 43.662 71 38 1 1 71 1 69 7.85e-14 70.5
MsaG045220 MtrunA17_Chr7g0246381 54.717 53 24 0 1 53 1 53 8.00e-14 65.9
MsaG045220 MtrunA17_Chr1g0204111 46.575 73 37 1 1 73 17 87 1.13e-13 70.1
MsaG045220 MtrunA17_Chr1g0208561 29.762 168 104 6 2 167 9 164 1.62e-13 69.3
MsaG045220 MtrunA17_Chr5g0404951 38.710 124 71 4 1 120 1 123 1.88e-13 69.3
MsaG045220 MtrunA17_Chr8g0377561 50.943 53 26 0 1 53 1 53 1.90e-13 65.9
MsaG045220 MtrunA17_Chr1g0175611 38.961 77 45 1 1 77 1 75 2.46e-13 67.4
MsaG045220 MtrunA17_Chr3g0089141 29.762 168 104 6 2 167 9 164 3.29e-13 68.6
MsaG045220 MtrunA17_Chr4g0061671 50.943 53 26 0 1 53 1 53 4.12e-13 67.4
MsaG045220 MtrunA17_Chr4g0057151 56.604 53 23 0 1 53 1 53 4.31e-13 67.8
MsaG045220 MtrunA17_Chr7g0220111 31.343 201 109 8 1 184 1 189 4.69e-13 68.2
MsaG045220 MtrunA17_Chr8g0351051 52.830 53 25 0 1 53 1 53 5.00e-13 68.2
MsaG045220 MtrunA17_Chr5g0420091 52.830 53 25 0 1 53 1 53 7.24e-13 67.0
MsaG045220 MtrunA17_Chr8g0355091 28.977 176 103 7 2 167 9 172 7.51e-13 67.4
MsaG045220 MtrunA17_Chr8g0379021 51.923 52 25 0 2 53 12 63 9.92e-13 65.9
MsaG045220 MtrunA17_Chr1g0166611 37.363 91 51 2 16 104 20 106 1.27e-12 64.7
MsaG045220 MtrunA17_Chr8g0351041 50.943 53 26 0 1 53 1 53 1.38e-12 65.1
MsaG045220 MtrunA17_Chr8g0357771 30.247 162 80 5 1 156 1 135 1.77e-12 65.5
MsaG045220 MtrunA17_Chr8g0351031 50.943 53 26 0 1 53 1 53 1.86e-12 66.2
MsaG045220 MtrunA17_Chr6g0455511 50.943 53 26 0 1 53 1 53 1.92e-12 66.6
MsaG045220 MtrunA17_Chr7g0220121 28.977 176 113 4 1 169 1 171 2.06e-12 66.6
MsaG045220 MtrunA17_Chr4g0052081 53.704 54 25 0 1 54 1 54 2.54e-12 64.7
MsaG045220 MtrunA17_Chr3g0122251 50.943 53 26 0 1 53 1 53 2.57e-12 66.2
MsaG045220 MtrunA17_Chr1g0208581 28.571 168 106 6 2 167 9 164 3.11e-12 65.5
MsaG045220 MtrunA17_Chr6g0474371 43.243 74 39 2 1 73 1 72 3.11e-12 65.1
MsaG045220 MtrunA17_Chr2g0333981 50.943 53 26 0 1 53 11 63 3.67e-12 64.3
MsaG045220 MtrunA17_Chr4g0018421 41.333 75 43 1 1 75 1 74 4.02e-12 64.7
MsaG045220 MtrunA17_Chr3g0099411 33.884 121 73 5 2 119 9 125 5.00e-12 64.7
MsaG045220 MtrunA17_Chr4g0074101 40.260 77 44 1 1 77 1 75 8.00e-12 63.9
MsaG045220 MtrunA17_Chr1g0175601 24.725 182 110 3 1 179 1 158 8.74e-12 63.2
MsaG045220 MtrunA17_Chr1g0206721 33.884 121 73 5 2 119 9 125 9.14e-12 64.3
MsaG045220 MtrunA17_Chr3g0099421 39.326 89 49 3 2 89 34 118 1.50e-11 63.9
MsaG045220 MtrunA17_Chr1g0180591 37.662 77 46 1 1 77 1 75 1.99e-11 62.4
MsaG045220 MtrunA17_Chr8g0376821 46.296 54 29 0 1 54 1 54 2.52e-11 62.0
MsaG045220 MtrunA17_Chr5g0419601 39.744 78 45 1 1 78 8 83 2.59e-11 63.2
MsaG045220 MtrunA17_Chr8g0356071 30.075 133 66 3 1 133 1 106 2.65e-11 61.6
MsaG045220 MtrunA17_Chr1g0206701 38.202 89 50 3 2 89 9 93 2.71e-11 63.2
MsaG045220 MtrunA17_Chr2g0317481 38.043 92 54 2 2 93 9 97 2.76e-11 62.8
MsaG045220 MtrunA17_Chr2g0317451 38.043 92 54 2 2 93 9 97 2.90e-11 62.8
MsaG045220 MtrunA17_Chr2g0292791 38.202 89 50 3 2 89 9 93 4.27e-11 62.4
MsaG045220 MtrunA17_Chr1g0187361 28.934 197 115 8 2 195 9 183 4.27e-11 62.4
MsaG045220 MtrunA17_Chr4g0052061 48.148 54 28 0 1 54 1 54 5.37e-11 60.5
MsaG045220 MtrunA17_Chr1g0169761 46.296 54 29 0 1 54 1 54 5.46e-11 60.8
MsaG045220 MtrunA17_Chr2g0325971 40.000 75 43 1 2 76 9 81 6.35e-11 61.6
MsaG045220 MtrunA17_Chr5g0422941 42.857 77 41 2 2 77 9 83 7.37e-11 61.6
MsaG045220 MtrunA17_Chr1g0188671 42.857 77 41 2 2 77 47 121 8.80e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045220 AT1G18750.4 52.824 301 123 6 1 296 1 287 1.31e-97 295
MsaG045220 AT1G18750.1 52.824 301 123 6 1 296 1 287 1.89e-97 295
MsaG045220 AT1G18750.3 47.605 334 123 7 1 296 1 320 1.21e-90 278
MsaG045220 AT2G03060.3 51.930 285 113 5 1 276 1 270 3.07e-89 273
MsaG045220 AT2G03060.7 51.930 285 113 5 1 276 1 270 3.07e-89 273
MsaG045220 AT2G03060.1 51.930 285 113 5 1 276 1 270 3.07e-89 273
MsaG045220 AT2G03060.2 51.930 285 113 5 1 276 1 270 3.17e-89 273
MsaG045220 AT1G69540.2 38.947 380 186 13 1 355 1 359 1.25e-70 225
MsaG045220 AT1G69540.1 43.322 307 144 8 1 292 1 292 1.69e-70 224
MsaG045220 AT1G18750.2 46.275 255 118 6 47 296 1 241 2.56e-66 213
MsaG045220 AT2G03060.6 43.627 204 91 5 82 276 24 212 1.70e-42 150
MsaG045220 AT2G03060.5 43.627 204 91 5 82 276 24 212 1.70e-42 150
MsaG045220 AT2G03060.4 43.627 204 91 5 82 276 24 212 1.70e-42 150
MsaG045220 AT1G18750.5 36.923 195 104 6 107 296 5 185 3.24e-29 114
MsaG045220 AT1G77980.1 36.735 196 108 6 1 181 1 195 1.85e-28 113
MsaG045220 AT1G18750.6 36.508 189 101 6 113 296 23 197 4.69e-27 108
MsaG045220 AT1G22130.1 33.500 200 118 5 1 186 1 199 1.06e-25 105
MsaG045220 AT1G77950.3 34.031 191 101 6 1 171 1 186 4.53e-24 99.8
MsaG045220 AT1G77950.2 34.031 191 101 6 1 171 1 186 4.53e-24 99.8
MsaG045220 AT1G77950.1 34.031 191 101 6 1 171 1 186 4.53e-24 99.8
MsaG045220 AT1G77950.4 34.031 191 101 6 1 171 1 186 4.53e-24 99.8
MsaG045220 AT1G69540.3 33.333 174 106 4 96 260 2 174 1.93e-21 92.4
MsaG045220 AT2G26320.1 47.561 82 43 0 1 82 17 98 4.32e-19 82.0
MsaG045220 AT2G03710.1 36.145 166 71 6 1 159 1 138 1.22e-16 79.3
MsaG045220 AT2G03710.2 36.145 166 71 6 1 159 1 138 1.26e-16 79.3
MsaG045220 AT4G24540.1 32.787 183 91 6 1 180 1 154 4.43e-16 77.0
MsaG045220 AT2G03710.3 36.145 166 71 6 1 159 1 138 4.59e-16 75.9
MsaG045220 AT5G13790.1 38.384 99 57 2 1 97 1 97 6.76e-16 77.4
MsaG045220 AT5G23260.3 47.887 71 37 0 1 71 1 71 1.87e-15 75.5
MsaG045220 AT5G23260.2 47.887 71 37 0 1 71 1 71 2.15e-15 75.5
MsaG045220 AT5G23260.4 47.887 71 37 0 1 71 17 87 2.71e-15 75.5
MsaG045220 AT5G23260.1 47.887 71 37 0 1 71 17 87 2.92e-15 75.5
MsaG045220 AT3G57390.3 42.353 85 47 1 1 85 1 83 3.31e-15 74.7
MsaG045220 AT3G57390.1 42.353 85 47 1 1 85 1 83 4.13e-15 74.7
MsaG045220 AT5G13790.2 43.056 72 39 1 1 72 1 70 5.56e-15 74.7
MsaG045220 AT3G57230.2 44.444 72 38 1 1 72 1 70 7.35e-15 71.6
MsaG045220 AT4G37435.1 26.531 196 108 6 1 178 1 178 9.85e-15 72.4
MsaG045220 AT2G22540.3 32.927 164 84 6 1 164 1 138 1.77e-14 72.8
MsaG045220 AT2G22540.1 32.927 164 84 6 1 164 1 138 1.77e-14 72.8
MsaG045220 AT1G26310.1 43.038 79 44 1 1 79 1 78 2.15e-14 72.8
MsaG045220 AT2G14210.1 43.590 78 43 1 1 78 1 77 2.20e-14 72.4
MsaG045220 AT2G14210.2 43.590 78 43 1 1 78 1 77 2.35e-14 72.4
MsaG045220 AT3G57230.1 44.444 72 38 1 1 72 1 70 2.46e-14 72.4
MsaG045220 AT3G57230.4 44.444 72 38 1 1 72 1 70 2.46e-14 72.4
MsaG045220 AT2G45650.1 30.000 190 114 6 1 180 1 181 2.75e-14 72.4
MsaG045220 AT4G37940.3 45.070 71 37 1 1 71 1 69 3.11e-14 70.5
MsaG045220 AT4G37940.4 45.070 71 37 1 1 71 1 69 3.88e-14 71.2
MsaG045220 AT4G37940.1 45.070 71 37 1 1 71 1 69 4.18e-14 71.2
MsaG045220 AT5G51870.2 45.333 75 39 1 1 75 1 73 4.25e-14 70.1
MsaG045220 AT1G69120.2 43.038 79 44 1 1 79 1 78 5.60e-14 71.2
MsaG045220 AT4G09960.2 43.662 71 38 1 1 71 1 69 5.80e-14 70.9
MsaG045220 AT2G45660.2 46.479 71 36 1 1 71 1 69 6.40e-14 69.3
MsaG045220 AT2G22540.2 52.830 53 25 0 1 53 1 53 7.38e-14 70.9
MsaG045220 AT1G69120.1 43.038 79 44 1 1 79 1 78 7.41e-14 71.2
MsaG045220 AT4G09960.1 43.662 71 38 1 1 71 1 69 8.21e-14 70.5
MsaG045220 AT5G51870.1 45.333 75 39 1 1 75 1 73 8.82e-14 70.1
MsaG045220 AT4G09960.6 43.662 71 38 1 1 71 2 70 8.84e-14 70.5
MsaG045220 AT4G09960.5 43.662 71 38 1 1 71 11 79 1.03e-13 70.5
MsaG045220 AT2G45660.1 46.479 71 36 1 1 71 1 69 1.05e-13 70.1
MsaG045220 AT1G71692.1 42.500 80 45 1 1 80 1 79 1.10e-13 69.7
MsaG045220 AT5G51870.5 45.333 75 39 1 1 75 24 96 1.19e-13 69.3
MsaG045220 AT3G58780.2 52.830 53 25 0 1 53 16 68 1.22e-13 70.5
MsaG045220 AT3G58780.1 52.830 53 25 0 1 53 16 68 1.22e-13 70.5
MsaG045220 AT2G42830.1 52.830 53 25 0 1 53 16 68 1.34e-13 70.1
MsaG045220 AT2G42830.2 52.830 53 25 0 1 53 16 68 1.45e-13 70.1
MsaG045220 AT5G51870.4 45.333 75 39 1 1 75 1 73 1.45e-13 69.7
MsaG045220 AT3G58780.5 52.830 53 25 0 1 53 22 74 1.48e-13 70.1
MsaG045220 AT4G09960.3 43.662 71 38 1 1 71 27 95 1.70e-13 70.1
MsaG045220 AT5G51870.3 45.333 75 39 1 1 75 11 83 1.78e-13 69.7
MsaG045220 AT3G58780.3 52.830 53 25 0 1 53 16 68 2.04e-13 70.1
MsaG045220 AT4G18960.1 53.846 52 24 0 2 53 18 69 2.23e-13 69.7
MsaG045220 AT5G20240.2 36.782 87 55 0 1 87 1 87 2.36e-13 67.4
MsaG045220 AT2G22630.1 43.750 80 41 2 1 78 1 78 2.64e-13 68.9
MsaG045220 AT2G22630.2 43.750 80 41 2 1 78 1 78 2.64e-13 68.9
MsaG045220 AT3G58780.4 52.830 53 25 0 1 53 16 68 3.02e-13 69.7
MsaG045220 AT5G20240.1 50.943 53 26 0 1 53 1 53 4.25e-13 68.2
MsaG045220 AT3G61120.1 56.604 53 23 0 1 53 1 53 4.27e-13 68.9
MsaG045220 AT5G62165.14 40.789 76 43 1 1 76 1 74 4.42e-13 65.5
MsaG045220 AT5G60910.1 45.070 71 38 1 1 71 1 70 4.57e-13 68.6
MsaG045220 AT4G11880.2 28.638 213 114 8 1 213 1 175 4.98e-13 67.4
MsaG045220 AT4G11880.3 28.638 213 114 8 1 213 1 175 4.98e-13 67.4
MsaG045220 AT4G09960.4 52.830 53 25 0 1 53 96 148 5.16e-13 69.7
MsaG045220 AT5G10140.2 52.830 53 25 0 1 53 1 53 5.37e-13 67.0
MsaG045220 AT3G54340.1 31.731 104 71 0 1 104 1 104 5.60e-13 68.2
MsaG045220 AT5G10140.1 52.830 53 25 0 1 53 1 53 6.07e-13 67.4
MsaG045220 AT3G30260.1 40.845 71 41 1 1 71 1 70 6.18e-13 68.6
MsaG045220 AT5G10140.3 52.830 53 25 0 1 53 1 53 6.33e-13 67.0
MsaG045220 AT5G10140.4 52.830 53 25 0 1 53 1 53 6.35e-13 67.0
MsaG045220 AT4G22950.3 27.072 181 105 3 1 181 1 154 1.19e-12 67.0
MsaG045220 AT4G22950.1 27.072 181 105 3 1 181 1 154 1.19e-12 67.0
MsaG045220 AT5G62165.17 29.282 181 102 4 1 181 1 155 1.41e-12 65.5
MsaG045220 AT5G62165.12 29.282 181 102 4 1 181 1 155 1.41e-12 65.5
MsaG045220 AT5G62165.11 29.282 181 102 4 1 181 1 155 1.41e-12 65.5
MsaG045220 AT5G62165.3 29.282 181 102 4 1 181 1 155 1.51e-12 66.6
MsaG045220 AT5G62165.1 29.282 181 102 4 1 181 1 155 1.51e-12 66.6
MsaG045220 AT5G62165.2 29.282 181 102 4 1 181 1 155 1.51e-12 66.6
MsaG045220 AT4G11880.4 52.830 53 25 0 1 53 1 53 2.47e-12 66.2
MsaG045220 AT4G11880.1 52.830 53 25 0 1 53 1 53 2.47e-12 66.2
MsaG045220 AT1G24260.3 50.943 53 26 0 1 53 1 53 2.51e-12 66.2
MsaG045220 AT1G24260.1 50.943 53 26 0 1 53 1 53 3.02e-12 66.2
MsaG045220 AT1G24260.2 50.943 53 26 0 1 53 1 53 3.06e-12 66.2
MsaG045220 AT5G62165.6 40.789 76 43 1 1 76 1 74 3.08e-12 64.3
MsaG045220 AT5G62165.9 40.789 76 43 1 1 76 1 74 3.08e-12 64.3
MsaG045220 AT5G62165.7 40.789 76 43 1 1 76 1 74 3.08e-12 64.3
MsaG045220 AT5G62165.8 40.789 76 43 1 1 76 1 74 3.08e-12 64.3
MsaG045220 AT5G62165.13 40.789 76 43 1 1 76 1 74 3.08e-12 64.3
MsaG045220 AT5G15800.1 50.943 53 26 0 1 53 1 53 3.59e-12 66.2
MsaG045220 AT5G62165.10 40.789 76 43 1 1 76 1 74 4.26e-12 65.1
MsaG045220 AT5G62165.4 40.789 76 43 1 1 76 1 74 4.26e-12 65.1
MsaG045220 AT5G15800.2 50.943 53 26 0 1 53 1 53 4.40e-12 66.2
MsaG045220 AT3G02310.2 50.943 53 26 0 1 53 1 53 5.11e-12 64.7
MsaG045220 AT4G22950.2 27.072 181 105 3 1 181 1 154 5.18e-12 64.3
MsaG045220 AT5G51860.1 28.723 188 98 6 1 168 1 172 6.46e-12 64.7
MsaG045220 AT5G51860.2 42.466 73 40 1 1 73 1 71 6.65e-12 64.7
MsaG045220 AT3G02310.1 50.943 53 26 0 1 53 1 53 7.35e-12 65.1
MsaG045220 AT5G65050.5 34.211 152 84 5 1 147 1 141 7.68e-12 63.9
MsaG045220 AT5G65050.2 43.662 71 39 1 1 71 1 70 9.04e-12 63.5
MsaG045220 AT2G34440.1 46.296 54 29 0 1 54 1 54 1.59e-11 62.8
MsaG045220 AT5G65050.3 43.662 71 39 1 1 71 1 70 1.82e-11 63.2
MsaG045220 AT5G65050.1 43.662 71 39 1 1 71 1 70 1.95e-11 62.8
MsaG045220 AT5G65070.4 47.170 53 28 0 1 53 1 53 2.50e-11 61.6
MsaG045220 AT3G66656.1 46.296 54 29 0 1 54 1 54 4.03e-11 61.6
MsaG045220 AT1G01530.1 49.057 53 27 0 1 53 6 58 4.26e-11 63.2
MsaG045220 AT5G65070.1 47.170 53 28 0 1 53 1 53 4.51e-11 62.0
MsaG045220 AT4G36590.1 43.662 71 38 1 2 72 7 75 5.35e-11 62.8
MsaG045220 AT5G65070.5 47.170 53 28 0 1 53 1 53 7.61e-11 61.6
MsaG045220 AT5G65070.3 47.170 53 28 0 1 53 1 53 8.00e-11 62.0
MsaG045220 AT5G65070.2 47.170 53 28 0 1 53 1 53 8.69e-11 62.0

Find 82 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCCACATGTCACTATTT+TGG 0.102186 8:+70110151 MsaT045220.1:CDS
TAAATTGAAGATAAAGAAAT+TGG 0.209657 8:+70107716 MsaT045220.1:CDS
GTTCTAATTGAGCAGGAAAA+TGG 0.237423 8:+70108384 MsaT045220.1:intron
CCAAGCTATTCTGGTTATAC+TGG 0.263985 8:+70110104 MsaT045220.1:CDS
TGCACATTTAAGTAAGGATT+TGG 0.267580 8:-70110212 None:intergenic
CCAAAATCACTATTTGAAAA+TGG 0.281681 8:+70110353 MsaT045220.1:CDS
TTGAAAATGGACACCAGTTC+TGG 0.282537 8:+70110366 MsaT045220.1:CDS
AACCAAAGTTTATGTCATTC+AGG 0.285801 8:+70109812 MsaT045220.1:CDS
CCCTTTCTTGTGGGCTAAGT+TGG 0.290811 8:-70108285 None:intergenic
TTGTAGACCAGACATCATTA+AGG 0.306825 8:-70109728 None:intergenic
ATTTCACTCCCAAGCTATTC+TGG 0.316741 8:+70110095 MsaT045220.1:CDS
GGTACCTTGAATGAGTTAAA+TGG 0.322272 8:+70110185 MsaT045220.1:CDS
GCCCACAAGAAAGGGCTAAA+AGG 0.346530 8:+70108293 MsaT045220.1:CDS
CGCATTCAAGTGACATTAGT+TGG 0.358372 8:-70109591 None:intergenic
TGTAAAGATACAAGATTTCC+TGG 0.370430 8:+70108571 MsaT045220.1:CDS
CCTTTCTTGTGGGCTAAGTT+GGG 0.379085 8:-70108284 None:intergenic
AGGAAAGCCTACGTTATTAC+AGG 0.394098 8:+70107857 MsaT045220.1:CDS
CACTGTTGCACATTTAAGTA+AGG 0.404816 8:-70110218 None:intergenic
AAGGGCAAAGTCATCCCTTC+TGG 0.405073 8:-70109709 None:intergenic
TCTCAGTTATTGGAGTAATC+TGG 0.414099 8:+70109343 MsaT045220.1:intron
ACTTTGCCCTTAATGATGTC+TGG 0.425266 8:+70109721 MsaT045220.1:CDS
ATCATGATCCAATTTCTTGA+AGG 0.431577 8:-70108549 None:intergenic
CCCAACTTAGCCCACAAGAA+AGG 0.433847 8:+70108284 MsaT045220.1:CDS
CTTGGAACATCTTAGACAAA+TGG 0.437086 8:+70109379 MsaT045220.1:CDS
GTTCACTAGGTAACATCAAC+TGG 0.439721 8:-70109792 None:intergenic
TGTAGACCAGACATCATTAA+GGG 0.442310 8:-70109727 None:intergenic
TTGATTTGCTATTGATTGGT+TGG 0.442413 8:-70110292 None:intergenic
CACCTGAATGACATAAACTT+TGG 0.443645 8:-70109814 None:intergenic
CAATCGATTGTCTATACAAA+AGG 0.445203 8:+70109424 MsaT045220.1:CDS
GACAAATGGAAGACTCGCTC+AGG 0.455975 8:+70109393 MsaT045220.1:CDS
TTATTTGATTTGCTATTGAT+TGG 0.458324 8:-70110296 None:intergenic
TTCACTTCCTCAAGATCTTG+TGG 0.459942 8:-70110263 None:intergenic
GATCATAATTGTTATTAACT+TGG 0.470376 8:-70110328 None:intergenic
AAGAATCTCAACCTTTGTCT+TGG 0.471192 8:+70109749 MsaT045220.1:CDS
TACAAGATTTCCTGGGTTCA+AGG 0.476006 8:+70108579 MsaT045220.1:CDS
ATTTCCATTTAACTCATTCA+AGG 0.477610 8:-70110189 None:intergenic
TTATACTGGGAACTGCAAAC+TGG 0.478109 8:+70110118 MsaT045220.1:CDS
TGGGAACTGCAAACTGGAGG+TGG 0.480619 8:+70110124 MsaT045220.1:CDS
AGATTCTCACTTGATGACTT+AGG 0.481817 8:-70108808 None:intergenic
CAGTATAACCAGAATAGCTT+GGG 0.488834 8:-70110103 None:intergenic
TCCTCAAGATCTTGTGGAGG+AGG 0.493605 8:-70110257 None:intergenic
CCAACTTAGCCCACAAGAAA+GGG 0.507371 8:+70108285 MsaT045220.1:CDS
CCAGTATAACCAGAATAGCT+TGG 0.510057 8:-70110104 None:intergenic
CATTTGTAGAATTCCAGAAC+TGG 0.522572 8:-70110379 None:intergenic
GTCACTTGAATGCGTTAACC+AGG 0.524046 8:+70109600 MsaT045220.1:CDS
CAGTTCTGGAATTCTACAAA+TGG 0.526268 8:+70110380 MsaT045220.1:CDS
TCTGGAGAAGATCAGTAACT+TGG 0.527978 8:+70109361 MsaT045220.1:CDS
ACTTATTCGAAGCGGAAAAG+TGG 0.527998 8:+70107762 MsaT045220.1:CDS
AAAACTACATGCTTACCCTA+TGG 0.529353 8:-70110439 None:intergenic
ATTGCTATGTATAACGAGAA+TGG 0.533675 8:+70110413 MsaT045220.1:CDS
CAAGCTATTCTGGTTATACT+GGG 0.535943 8:+70110105 MsaT045220.1:CDS
CCTTTGTCTTGGCTTCTAAG+TGG 0.542337 8:+70109760 MsaT045220.1:CDS
AAGTGAGAATCTTACAAGCT+CGG 0.547873 8:+70108819 MsaT045220.1:CDS
CCCTCCTCCACAAGATCTTG+AGG 0.562025 8:+70110256 MsaT045220.1:CDS
ATAACGAGAATGGTTACCAT+AGG 0.564017 8:+70110423 MsaT045220.1:CDS
ATTGATTCATAGTTACCAGA+AGG 0.565773 8:+70109694 MsaT045220.1:intron
CTGTTGGAGAAAACATGAGA+AGG 0.576749 8:-70107836 None:intergenic
AATTCTACAAATGGCTCTTG+TGG 0.581991 8:+70110389 MsaT045220.1:CDS
CTTATTCGAAGCGGAAAAGT+GGG 0.585417 8:+70107763 MsaT045220.1:CDS
CAATCACTTACCTTGAACCC+AGG 0.589742 8:-70108589 None:intergenic
CGTTCTCCCTGTAATAACGT+AGG 0.597125 8:-70107864 None:intergenic
GTAAAGATACAAGATTTCCT+GGG 0.597719 8:+70108572 MsaT045220.1:CDS
TAACGAGAATGGTTACCATA+GGG 0.597927 8:+70110424 MsaT045220.1:CDS
CCTCAAGATCTTGTGGAGGA+GGG 0.600496 8:-70110256 None:intergenic
GACATAAACTTTGGTTCACT+AGG 0.613858 8:-70109805 None:intergenic
CAATAGCAAATCAAATAACG+TGG 0.614557 8:+70110301 MsaT045220.1:CDS
ACTTCCTCAAGATCTTGTGG+AGG 0.620137 8:-70110260 None:intergenic
GGGAACTGCAAACTGGAGGT+GGG 0.623032 8:+70110125 MsaT045220.1:CDS
AATAACGTAGGCTTTCCTGT+TGG 0.623309 8:-70107852 None:intergenic
TTGATTCATAGTTACCAGAA+GGG 0.636080 8:+70109695 MsaT045220.1:intron
CAGAAATACAACAGAGACTG+AGG 0.637183 8:+70108846 MsaT045220.1:CDS
ATAGCTTGGGAGTGAAATGT+CGG 0.637360 8:-70110090 None:intergenic
ATATTGCAGCAGTCAAACAG+TGG 0.648674 8:+70108678 MsaT045220.1:intron
TACTGGGAACTGCAAACTGG+AGG 0.648889 8:+70110121 MsaT045220.1:CDS
ACAAATGGAAGACTCGCTCA+GGG 0.649235 8:+70109394 MsaT045220.1:CDS
TGACCCAAAATAGTGACATG+TGG 0.657881 8:-70110155 None:intergenic
GCTTCGAATAAGTCACATGT+CGG 0.667125 8:-70107752 None:intergenic
GGAAAGCCTACGTTATTACA+GGG 0.679926 8:+70107858 MsaT045220.1:CDS
GACATGTGACTTATTCGAAG+CGG 0.683369 8:+70107754 MsaT045220.1:CDS
CCACTTAGAAGCCAAGACAA+AGG 0.694579 8:-70109760 None:intergenic
TTGCAGCAGTCAAACAGTGG+AGG 0.697141 8:+70108681 MsaT045220.1:intron
CGTTATTACAGGGAGAACGA+AGG 0.718082 8:+70107868 MsaT045220.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 70107705 70110457 70107705 ID=MsaG045220
Chr8 mRNA 70107705 70110457 70107705 ID=MsaT045220.1;Parent=MsaG045220
Chr8 exon 70107705 70107889 70107705 ID=MsaT045220.1.exon1;Parent=MsaT045220.1
Chr8 CDS 70107705 70107889 70107705 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70108255 70108314 70108255 ID=MsaT045220.1.exon2;Parent=MsaT045220.1
Chr8 CDS 70108255 70108314 70108255 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70108399 70108417 70108399 ID=MsaT045220.1.exon3;Parent=MsaT045220.1
Chr8 CDS 70108399 70108417 70108399 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70108536 70108600 70108536 ID=MsaT045220.1.exon4;Parent=MsaT045220.1
Chr8 CDS 70108536 70108600 70108536 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70108687 70108702 70108687 ID=MsaT045220.1.exon5;Parent=MsaT045220.1
Chr8 CDS 70108687 70108702 70108687 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70108806 70108867 70108806 ID=MsaT045220.1.exon6;Parent=MsaT045220.1
Chr8 CDS 70108806 70108867 70108806 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70109349 70109445 70109349 ID=MsaT045220.1.exon7;Parent=MsaT045220.1
Chr8 CDS 70109349 70109445 70109349 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70109574 70109621 70109574 ID=MsaT045220.1.exon8;Parent=MsaT045220.1
Chr8 CDS 70109574 70109621 70109574 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70109706 70109833 70109706 ID=MsaT045220.1.exon9;Parent=MsaT045220.1
Chr8 CDS 70109706 70109833 70109706 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Chr8 exon 70110058 70110457 70110058 ID=MsaT045220.1.exon10;Parent=MsaT045220.1
Chr8 CDS 70110058 70110457 70110058 ID=cds.MsaT045220.1;Parent=MsaT045220.1
Gene Sequence

>MsaT045220.1

ATGGGGAGAGTTAAATTGAAGATAAAGAAATTGGAGAGCACAAGCAACCGACATGTGACTTATTCGAAGCGGAAAAGTGGGATCTTGAAGAAAGCAAAAGAGTTATCAATTCTATGTGATATAGATATTCTCCTTCTCATGTTTTCTCCAACAGGAAAGCCTACGTTATTACAGGGAGAACGAAGCAATATGGAGGAGATCATTGCAAAATTTGCCCAACTTAGCCCACAAGAAAGGGCTAAAAGGAAAATGGAAAGTCTTGAAGCACTGAAAAAAACCTTCAAGAAATTGGATCATGATGTAAAGATACAAGATTTCCTGGGTTCAAGCAGTCAAACAGTGGAGGACCTAAGTCATCAAGTGAGAATCTTACAAGCTCGGCTCACAGAAATACAACAGAGACTGAGTTATTGGAGTAATCTGGAGAAGATCAGTAACTTGGAACATCTTAGACAAATGGAAGACTCGCTCAGGGAATCAATCAATCGATTGTCTATACAAAAGGAAAATCTCGAAAAGCACCAACTAATGTCACTTGAATGCGTTAACCAGTTACCAGAAGGGATGACTTTGCCCTTAATGATGTCTGGTCTACAAGAATCTCAACCTTTGTCTTGGCTTCTAAGTGGTGATAATCACCAGTTGATGTTACCTAGTGAACCAAAGTTTATGTCATTCAGTGACAATGTCAATAGAGATGTTGAGTGCTCAACCGACATTTCACTCCCAAGCTATTCTGGTTATACTGGGAACTGCAAACTGGAGGTGGGAAGCTCTCCACATGTCACTATTTTGGGTCAGGGAGGTGGTACCTTGAATGAGTTAAATGGAAATCCAAATCCTTACTTAAATGTGCAACAGTGTCAGCAGTTTGCATACCCTCCTCCACAAGATCTTGAGGAAGTGAAACATCACCAACCAATCAATAGCAAATCAAATAACGTGGACTACCAAGTTAATAACAATTATGATCTGCCAAAATCACTATTTGAAAATGGACACCAGTTCTGGAATTCTACAAATGGCTCTTGTGGTATTGCTATGTATAACGAGAATGGTTACCATAGGGTAAGCATGTAG
Protein sequence

>MsaT045220.1

MGRVKLKIKKLESTSNRHVTYSKRKSGILKKAKELSILCDIDILLLMFSPTGKPTLLQGERSNMEEIIAKFAQLSPQERAKRKMESLEALKKTFKKLDHDVKIQDFLGSSSQTVEDLSHQVRILQARLTEIQQRLSYWSNLEKISNLEHLRQMEDSLRESINRLSIQKENLEKHQLMSLECVNQLPEGMTLPLMMSGLQESQPLSWLLSGDNHQLMLPSEPKFMSFSDNVNRDVECSTDISLPSYSGYTGNCKLEVGSSPHVTILGQGGGTLNELNGNPNPYLNVQQCQQFAYPPPQDLEEVKHHQPINSKSNNVDYQVNNNYDLPKSLFENGHQFWNSTNGSCGIAMYNENGYHRVSM*