AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045222


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045222 MtrunA17_Chr1g0203051 52.514 179 75 2 1 177 1 171 6.25e-51 182
MsaG045222 MtrunA17_Chr1g0203051 50.667 75 37 0 177 251 547 621 1.70e-16 82.0
MsaG045222 MtrunA17_Chr8g0389811 47.863 117 59 1 61 175 58 174 3.78e-34 127
MsaG045222 MtrunA17_Chr2g0331371 42.105 114 64 1 68 179 36 149 1.31e-23 98.2
MsaG045222 MtrunA17_Chr6g0473421 37.984 129 73 3 35 160 6 130 2.01e-23 95.9
MsaG045222 MtrunA17_Chr1g0171741 34.503 171 103 5 11 177 59 224 2.11e-20 90.5
MsaG045222 MtrunA17_Chr1g0172931 41.818 110 62 1 60 167 49 158 1.83e-19 85.1
MsaG045222 MtrunA17_Chr2g0308841 42.529 87 50 0 177 263 140 226 5.42e-15 74.7
MsaG045222 MtrunA17_Chr2g0287651 36.364 110 68 1 70 177 58 167 8.03e-14 73.9
MsaG045222 MtrunA17_Chr7g0228051 50.000 68 31 1 32 96 18 85 1.82e-11 60.1
MsaG045222 MtrunA17_Chr1g0203721 29.218 243 137 11 49 266 393 625 8.78e-11 64.3
MsaG045222 MtrunA17_Chr5g0448511 26.562 192 137 2 192 379 325 516 9.24e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045222 AT2G27110.2 35.000 100 56 2 185 278 503 599 2.87e-11 66.2
MsaG045222 AT2G27110.1 35.000 100 56 2 185 278 503 599 2.87e-11 66.2
MsaG045222 AT2G27110.3 35.000 100 56 2 185 278 358 454 3.23e-11 65.9

Find 92 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTAAATGTCAACCGTTCT+TGG 0.283454 8:-70190292 None:intergenic
CAACTAAACGATCCTGAAAA+TGG 0.285819 8:-70188794 None:intergenic
CCTCTGAACCTTCCGCATTA+AGG 0.302211 8:-70188472 None:intergenic
CGTTCCATCACAAGACATTC+TGG 0.328402 8:-70190398 None:intergenic
GCTTAATGATTCCAATGTTT+CGG 0.343715 8:-70190606 None:intergenic
TGCAAAAGATTCGAGACATT+TGG 0.377751 8:+70190320 MsaT045222.1:CDS
GATTCCATCTTTAGCACGTT+TGG 0.386420 8:-70190426 None:intergenic
GACATTTACAGTTCGTTGTA+TGG 0.387972 8:+70188678 MsaT045222.1:CDS
CATCCACATATGCCACATTT+CGG 0.392359 8:-70190770 None:intergenic
TTTGTCATAGAGAAGGTTAC+AGG 0.397074 8:+70188919 MsaT045222.1:CDS
AGGGTATTTGAAAACATTGT+AGG 0.400513 8:-70190237 None:intergenic
TATACACAAAACCATGGAAA+TGG 0.430787 8:+70190571 MsaT045222.1:CDS
ATGATGAAGACTCGGAAGAT+TGG 0.445167 8:+70190879 MsaT045222.1:CDS
AAAGCCGTGTTGTTAAGAAC+AGG 0.447436 8:+70188868 MsaT045222.1:CDS
TAAGCAGTTTAGGCCCGTAT+TGG 0.451250 8:+70190160 MsaT045222.1:intron
ACCAGTTATGTCGCTTACAT+TGG 0.451744 8:-70188441 None:intergenic
GATGTTCGCAACTGATGAAC+CGG 0.455923 8:+70191087 MsaT045222.1:CDS
ACCCTAGGGATGATATTCAT+TGG 0.456636 8:+70190255 MsaT045222.1:CDS
CCCAATGTAAGCGACATAAC+TGG 0.464104 8:+70188440 MsaT045222.1:CDS
CCAGTTATGTCGCTTACATT+GGG 0.465368 8:-70188440 None:intergenic
TCACATATCAATGATGATCA+AGG 0.468748 8:+70190854 MsaT045222.1:CDS
TCTTCATCTCGTAATGTAAG+TGG 0.469724 8:+70190728 MsaT045222.1:CDS
GAGAGCAGAAGGCAACTAGT+AGG 0.491224 8:+70188970 MsaT045222.1:CDS
AACTAGTAGGTGTGGTTGCA+TGG 0.492607 8:+70188983 MsaT045222.1:CDS
AAACATTGGAATCATTAAGC+AGG 0.500216 8:+70190609 MsaT045222.1:CDS
ACATTTACAGTTCGTTGTAT+GGG 0.501983 8:+70188679 MsaT045222.1:CDS
CATAGAGAAGGTTACAGGGT+TGG 0.507010 8:+70188924 MsaT045222.1:CDS
CCGACTAGATCTCTCCAATA+CGG 0.511443 8:-70190174 None:intergenic
CATGGACAATAGCTATTGTT+CGG 0.512713 8:+70188620 MsaT045222.1:CDS
CCTTAAAATTAATTCTGTCA+AGG 0.525060 8:-70188743 None:intergenic
TGGACCAAACGTGCTAAAGA+TGG 0.531683 8:+70190422 MsaT045222.1:CDS
TGCGGAAGGTTCAGAGGTAG+AGG 0.537083 8:+70188478 MsaT045222.1:CDS
GCAACTGATGAACCGGAGAA+TGG 0.538478 8:+70191094 MsaT045222.1:CDS
ACCTTTGTTTGTCATAGAGA+AGG 0.542041 8:+70188912 MsaT045222.1:CDS
AAGCGGAGTTCGCCGAAATG+TGG 0.543560 8:+70190758 MsaT045222.1:CDS
TCGCCGAAATGTGGCATATG+TGG 0.547405 8:+70190767 MsaT045222.1:CDS
GTGCAACTTTGACGATGAAA+CGG 0.547511 8:+70188701 MsaT045222.1:CDS
TCCACACTTCAACACGGCAT+TGG 0.549156 8:-70190537 None:intergenic
TCTGTCACTGAACCGACCAC+GGG 0.550131 8:-70190650 None:intergenic
ATGTGGCATATGTGGATGTA+AGG 0.551576 8:+70190775 MsaT045222.1:CDS
TTCTGTCACTGAACCGACCA+CGG 0.554234 8:-70190651 None:intergenic
CGATAGGAAAAGAGAGCAGA+AGG 0.554726 8:+70188959 MsaT045222.1:CDS
AATTTGTACGGTTGTGTACG+TGG 0.562187 8:+70188831 MsaT045222.1:CDS
CTTCGTAGGCGACAAGGCTG+CGG 0.566588 8:-70190804 None:intergenic
AACATTGGAATCATTAAGCA+GGG 0.567060 8:+70190610 MsaT045222.1:CDS
CCGCAACGGCTTAAGAAAAG+AGG 0.575682 8:+70190689 MsaT045222.1:CDS
CAACGGCTTAAGAAAAGAGG+TGG 0.576611 8:+70190692 MsaT045222.1:CDS
ATCCAATGAATATCATCCCT+AGG 0.577004 8:-70190257 None:intergenic
ACATACGTAGTTACAAATGC+AGG 0.577104 8:-70190485 None:intergenic
GGAGAGTGATGTATGTTCCT+CGG 0.577170 8:+70188641 MsaT045222.1:CDS
TGAAACGGCGGTTGATTGCA+TGG 0.577332 8:+70188716 MsaT045222.1:CDS
GGTCATCATACACCATTCTC+CGG 0.578756 8:-70191106 None:intergenic
CGACTAGATCTCTCCAATAC+GGG 0.579381 8:-70190173 None:intergenic
TCCAATGAATATCATCCCTA+GGG 0.582068 8:-70190256 None:intergenic
CACATATCAATGATGATCAA+GGG 0.585150 8:+70190855 MsaT045222.1:CDS
CAGAAGGCAACTAGTAGGTG+TGG 0.587238 8:+70188975 MsaT045222.1:CDS
GTACACGTTGACGCTGGTAG+CGG 0.591900 8:+70189017 MsaT045222.1:CDS
TTGACGCTGGTAGCGGTCGA+TGG 0.592302 8:+70189024 MsaT045222.1:CDS
TAAATATATACACAAAACCA+TGG 0.595738 8:+70190565 MsaT045222.1:CDS
CCTTAATGCGGAAGGTTCAG+AGG 0.595778 8:+70188472 MsaT045222.1:CDS
CGTGTTGTTAAGAACAGGGA+TGG 0.599865 8:+70188873 MsaT045222.1:CDS
TTGTCATAGAGAAGGTTACA+GGG 0.601328 8:+70188920 MsaT045222.1:CDS
GTTGATGAATTGCCCGTGGT+CGG 0.602393 8:+70190638 MsaT045222.1:CDS
CATCCCTGTTCTTAACAACA+CGG 0.608258 8:-70188872 None:intergenic
GCCAATGCCGTGTTGAAGTG+TGG 0.608683 8:+70190536 MsaT045222.1:CDS
TAAAGTCTTCGTAGGCGACA+AGG 0.612256 8:-70190810 None:intergenic
GGATGCAACCTTAATGCGGA+AGG 0.614546 8:+70188464 MsaT045222.1:CDS
AAAGTTTAGGTAATCCGCAA+CGG 0.615547 8:+70190675 MsaT045222.1:CDS
TCTAGTCGGTGCAAAGTCGA+AGG 0.615809 8:+70190188 MsaT045222.1:CDS
ACCTTCTCTATGACAAACAA+AGG 0.615884 8:-70188913 None:intergenic
CGTTGAGCGATGTAAGAGGA+TGG 0.618826 8:+70190514 MsaT045222.1:CDS
TGTTCGTTGAGCGATGTAAG+AGG 0.622081 8:+70190510 MsaT045222.1:CDS
ATGTACATTAAAGTCTTCGT+AGG 0.624035 8:-70190818 None:intergenic
TTGGAAGTAATGCTGCCGAT+AGG 0.626637 8:+70188943 MsaT045222.1:CDS
TAGTTACAAATGCAGGATCA+CGG 0.636049 8:-70190478 None:intergenic
GTCTTCGACATCTTCATCCG+AGG 0.643998 8:-70188658 None:intergenic
AATGTAAGCGACATAACTGG+TGG 0.647899 8:+70188443 MsaT045222.1:CDS
GGAAGATTGGAACACACCCA+TGG 0.654797 8:+70190892 MsaT045222.1:CDS
TCAAGGGTATGATGAAGACT+CGG 0.658393 8:+70190871 MsaT045222.1:CDS
AGGATCTCCACACTTCAACA+CGG 0.660448 8:-70190543 None:intergenic
ATTCAAGTACACGTTGACGC+TGG 0.666323 8:+70189011 MsaT045222.1:CDS
ACTGCCAGAATGTCTTGTGA+TGG 0.666806 8:+70190394 MsaT045222.1:CDS
TGGTGGATGCAACCTTAATG+CGG 0.674046 8:+70188460 MsaT045222.1:CDS
AAGCCGTGTTGTTAAGAACA+GGG 0.681295 8:+70188869 MsaT045222.1:CDS
GTCGAAGGTGATGTGCGCAG+TGG 0.682048 8:+70190203 MsaT045222.1:CDS
ACATGTAAGATATGTTCACA+AGG 0.686637 8:-70190347 None:intergenic
CCGTATTGGAGAGATCTAGT+CGG 0.690217 8:+70190174 MsaT045222.1:CDS
TGAAGTTGATGAATTGCCCG+TGG 0.692394 8:+70190634 MsaT045222.1:CDS
CAACTTTGACGATGAAACGG+CGG 0.704083 8:+70188704 MsaT045222.1:CDS
AATGTCTTGTGATGGAACGA+TGG 0.705741 8:+70190402 MsaT045222.1:CDS
ACATTGGAATCATTAAGCAG+GGG 0.706809 8:+70190611 MsaT045222.1:CDS
TAGGGTCGGATGAAATCATG+CGG 0.716997 8:+70188769 MsaT045222.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 70188404 70191123 70188404 ID=MsaG045222
Chr8 mRNA 70188404 70191123 70188404 ID=MsaT045222.1;Parent=MsaG045222
Chr8 exon 70188404 70188499 70188404 ID=MsaT045222.1.exon1;Parent=MsaT045222.1
Chr8 CDS 70188404 70188499 70188404 ID=cds.MsaT045222.1;Parent=MsaT045222.1
Chr8 exon 70188618 70189045 70188618 ID=MsaT045222.1.exon2;Parent=MsaT045222.1
Chr8 CDS 70188618 70189045 70188618 ID=cds.MsaT045222.1;Parent=MsaT045222.1
Chr8 exon 70190172 70190913 70190172 ID=MsaT045222.1.exon3;Parent=MsaT045222.1
Chr8 CDS 70190172 70190913 70190172 ID=cds.MsaT045222.1;Parent=MsaT045222.1
Chr8 exon 70191070 70191123 70191070 ID=MsaT045222.1.exon4;Parent=MsaT045222.1
Chr8 CDS 70191070 70191123 70191070 ID=cds.MsaT045222.1;Parent=MsaT045222.1
Gene Sequence

>MsaT045222.1

ATGGATATTCATGAAATTCTAGAAGTTTTCGGAATTCCCAATGTAAGCGACATAACTGGTGGATGCAACCTTAATGCGGAAGGTTCAGAGGTAGAGGACATGGACAATAGCTATTGTTCGGAGAGTGATGTATGTTCCTCGGATGAAGATGTCGAAGACATTTACAGTTCGTTGTATGGGTGCAACTTTGACGATGAAACGGCGGTTGATTGCATGGCTGACCTTGACAGAATTAATTTTAAGGAAATAGGGTCGGATGAAATCATGCGGTACCATTTTCAGGATCGTTTAGTTGCATTCAAGTTTTACAATTTGTACGGTTGTGTACGTGGATTTTCCGGTAGAAAAAGCCGTGTTGTTAAGAACAGGGATGGCGATGTTGTTCAACAAACCTTTGTTTGTCATAGAGAAGGTTACAGGGTTGGAAGTAATGCTGCCGATAGGAAAAGAGAGCAGAAGGCAACTAGTAGGTGTGGTTGCATGGCAAAAATTCAAGTACACGTTGACGCTGGTAGCGGTCGATGGCCCGTATTGGAGAGATCTAGTCGGTGCAAAGTCGAAGGTGATGTGCGCAGTGGTTCCATTTTTACCTACAATGTTTTCAAATACCCTAGGGATGATATTCATTGGATCGTGACATTTTGCCAAGAACGGTTGACATTTAAATGTTCATGCAAAAGATTCGAGACATTTGGTATTCCTTGTGAACATATCTTACATGTTCTAGTGCATTTGAACATTGTTGTACTGCCAGAATGTCTTGTGATGGAACGATGGACCAAACGTGCTAAAGATGGAATCAATGCTCAAAATGCTAATAGTTCAAGTCACCGTGATCCTGCATTTGTAACTACGTATGTGATGTTCGTTGAGCGATGTAAGAGGATGGCCAATGCCGTGTTGAAGTGTGGAGATCCTAAATATATACACAAAACCATGGAAATGGTTGAAAAACACACCGAAACATTGGAATCATTAAGCAGGGGTGAAGTTGATGAATTGCCCGTGGTCGGTTCAGTGACAGAAAAAAGTTTAGGTAATCCGCAACGGCTTAAGAAAAGAGGTGGAGCAGCAGCTGCGTCTTCATCTCGTAATGTAAGTGGAAATAAAAAGCGGAGTTCGCCGAAATGTGGCATATGTGGATGTAAGGTCACAACCGCAGCCTTGTCGCCTACGAAGACTTTAATGTACATACGTAATCATTCATCACATATCAATGATGATCAAGGGTATGATGAAGACTCGGAAGATTGGAACACACCCATGTTTGATGTTGCGTTGAAGATGTTCGCAACTGATGAACCGGAGAATGGTGTATGA

Protein sequence

>MsaT045222.1

MDIHEILEVFGIPNVSDITGGCNLNAEGSEVEDMDNSYCSESDVCSSDEDVEDIYSSLYGCNFDDETAVDCMADLDRINFKEIGSDEIMRYHFQDRLVAFKFYNLYGCVRGFSGRKSRVVKNRDGDVVQQTFVCHREGYRVGSNAADRKREQKATSRCGCMAKIQVHVDAGSGRWPVLERSSRCKVEGDVRSGSIFTYNVFKYPRDDIHWIVTFCQERLTFKCSCKRFETFGIPCEHILHVLVHLNIVVLPECLVMERWTKRAKDGINAQNANSSSHRDPAFVTTYVMFVERCKRMANAVLKCGDPKYIHKTMEMVEKHTETLESLSRGEVDELPVVGSVTEKSLGNPQRLKKRGGAAAASSSRNVSGNKKRSSPKCGICGCKVTTAALSPTKTLMYIRNHSSHINDDQGYDEDSEDWNTPMFDVALKMFATDEPENGV*