AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045294


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045294 MtrunA17_Chr4g0061561 95.752 306 10 3 1 304 2 306 0.0 581
MsaG045294 MtrunA17_Chr3g0086961 44.022 368 126 16 4 297 3 364 5.94e-59 193
MsaG045294 MtrunA17_Chr7g0219461 61.017 118 45 1 1 117 1 118 1.93e-43 152
MsaG045294 MtrunA17_Chr6g0454781 71.429 84 24 0 38 121 31 114 1.63e-37 136
MsaG045294 MtrunA17_Chr8g0376571 69.863 73 22 0 46 118 51 123 2.89e-28 114
MsaG045294 MtrunA17_Chr8g0360441 52.809 89 42 0 29 117 27 115 2.78e-22 96.3
MsaG045294 MtrunA17_Chr4g0040921 54.321 81 37 0 49 129 15 95 4.22e-22 94.7
MsaG045294 MtrunA17_Chr2g0325171 61.765 68 26 0 50 117 18 85 2.34e-21 92.4
MsaG045294 MtrunA17_Chr2g0316391 58.824 68 28 0 50 117 14 81 2.39e-20 89.0
MsaG045294 MtrunA17_Chr1g0205131 50.000 60 30 0 55 114 108 167 1.35e-12 67.8
MsaG045294 MtrunA17_Chr7g0220931 49.123 57 29 0 58 114 122 178 2.85e-12 66.6
MsaG045294 MtrunA17_Chr6g0456881 48.276 58 30 0 57 114 127 184 4.43e-12 66.2
MsaG045294 MtrunA17_Chr4g0062691 48.276 58 30 0 57 114 133 190 6.20e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045294 AT5G60970.1 80.682 88 16 1 39 125 45 132 5.46e-46 159
MsaG045294 AT3G02150.1 43.062 209 101 7 10 200 28 236 3.59e-42 147
MsaG045294 AT3G02150.2 43.062 209 101 7 10 200 28 236 1.42e-41 147
MsaG045294 AT5G08070.2 50.676 148 64 4 21 168 2 140 1.68e-40 142
MsaG045294 AT5G08070.1 50.676 148 64 4 21 168 2 140 1.68e-40 142
MsaG045294 AT1G30210.2 55.752 113 44 3 8 118 4 112 3.84e-30 116
MsaG045294 AT1G30210.1 55.752 113 44 3 8 118 4 112 3.84e-30 116
MsaG045294 AT4G18390.1 68.493 73 23 0 46 118 32 104 3.33e-28 112
MsaG045294 AT4G18390.2 68.493 73 23 0 46 118 32 104 3.33e-28 112
MsaG045294 AT1G53230.1 57.500 80 31 1 50 126 43 122 2.54e-23 99.4
MsaG045294 AT1G53230.2 57.500 80 31 1 50 126 43 122 2.54e-23 99.4
MsaG045294 AT3G15030.2 61.765 68 26 0 50 117 39 106 3.85e-22 96.3
MsaG045294 AT3G15030.4 61.765 68 26 0 50 117 39 106 3.85e-22 96.3
MsaG045294 AT3G15030.3 61.765 68 26 0 50 117 39 106 3.85e-22 96.3
MsaG045294 AT3G15030.1 61.765 68 26 0 50 117 39 106 3.85e-22 96.3
MsaG045294 AT2G31070.1 60.606 66 26 0 49 114 22 87 2.39e-20 90.5
MsaG045294 AT1G68800.1 46.269 67 36 0 48 114 104 170 1.12e-14 73.9
MsaG045294 AT1G68800.2 46.269 67 36 0 48 114 104 170 1.27e-14 73.9
MsaG045294 AT1G67260.2 41.429 70 41 0 45 114 76 145 6.24e-14 72.0
MsaG045294 AT1G67260.3 41.429 70 41 0 45 114 76 145 6.24e-14 72.0
MsaG045294 AT1G67260.1 41.429 70 41 0 45 114 76 145 7.73e-14 71.6
MsaG045294 AT3G18550.1 39.024 82 49 1 33 114 126 206 2.21e-12 67.8
MsaG045294 AT3G18550.4 39.024 82 49 1 33 114 126 206 2.21e-12 67.8
MsaG045294 AT3G18550.2 39.024 82 49 1 33 114 126 206 2.25e-12 67.8
MsaG045294 AT3G18550.3 39.024 82 49 1 33 114 126 206 2.89e-12 67.4

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCTAAACGGCGAATTTAT+TGG 0.202116 8:-70944545 None:intergenic
GAAATAAATTACCATGATTA+TGG 0.231308 8:-70944277 None:intergenic
ACTCTATCTTTATCACAATT+TGG 0.233467 8:+70944251 MsaT045294.1:CDS
CGTGACACTCTCACTATTCT+TGG 0.252002 8:-70943834 None:intergenic
CTTGAAGGATCCAATTTATA+TGG 0.254937 8:-70944227 None:intergenic
TACTACCTGGTTGCTGAAAT+AGG 0.258592 8:-70944085 None:intergenic
CTACCATAACTACCCATTTC+TGG 0.267833 8:-70944618 None:intergenic
AGCAAAGTTTGCACAATTAG+AGG 0.305586 8:+70943882 MsaT045294.1:CDS
TGATGTAGCTATTGCTTTGT+TGG 0.307657 8:-70943787 None:intergenic
GTGAGAGTGTCACGTTCTTT+AGG 0.317533 8:+70943843 MsaT045294.1:CDS
GATGATAATAACACCAGAAA+TGG 0.330153 8:+70944605 MsaT045294.1:CDS
AATTCACTACTTTCCATTGA+TGG 0.341892 8:-70944449 None:intergenic
GATTAAGAGACCGTAGGATT+AGG 0.348209 8:+70943904 MsaT045294.1:CDS
TGTGAATTACCAGATGAATT+AGG 0.362279 8:-70944365 None:intergenic
ACTACCTGGTTGCTGAAATA+GGG 0.363637 8:-70944084 None:intergenic
AATACTAATTATTCATCAAA+TGG 0.403938 8:+70944194 MsaT045294.1:CDS
CTAGTACTTATTTGATAAGA+AGG 0.404054 8:-70944299 None:intergenic
TTTATTGGAAGATGAATAGT+TGG 0.406511 8:-70944530 None:intergenic
AGAAGCTGAAAGTTCAGAAA+AGG 0.433467 8:+70944160 MsaT045294.1:CDS
TACTCTGAATGCTCCATCAA+TGG 0.441615 8:+70944436 MsaT045294.1:CDS
GTTCAAGATTTGAACATGGT+TGG 0.449373 8:-70944483 None:intergenic
TATGGTATGAATCAAACTAA+AGG 0.451228 8:+70944134 MsaT045294.1:CDS
TTGACAGTACCTAATTCATC+TGG 0.458238 8:+70944356 MsaT045294.1:CDS
AACCTAGCAAAGTTGTTGAA+TGG 0.462940 8:+70943985 MsaT045294.1:CDS
AGTGTTGGATGGCGTCTAAA+CGG 0.463809 8:-70944557 None:intergenic
ATCATCTGAATCAAGAAGCT+TGG 0.472188 8:-70944588 None:intergenic
TGAACATGGTTGGATTGTCT+TGG 0.473682 8:-70944473 None:intergenic
GGATCCAATTTATATGGAAA+AGG 0.478861 8:-70944221 None:intergenic
ATGATAATAACACCAGAAAT+GGG 0.506853 8:+70944606 MsaT045294.1:CDS
ATGAAAGAACCATTACTTGA+GGG 0.508573 8:-70944506 None:intergenic
AGAAGGTGAACATGGAAGAA+TGG 0.508923 8:+70943749 MsaT045294.1:CDS
TGTTGTTGAGTGAAACTTTG+AGG 0.510713 8:-70944059 None:intergenic
GATAAAGATAGAGTACTTGA+AGG 0.512410 8:-70944242 None:intergenic
AACCATTCAACAACTTTGCT+AGG 0.525009 8:-70943987 None:intergenic
GAAGCTGAAAGTTCAGAAAA+GGG 0.530447 8:+70944161 MsaT045294.1:CDS
AGCTTGGAACTAAACAGTGT+TGG 0.544286 8:-70944572 None:intergenic
TATGATCTTCAAGACAGACT+TGG 0.562288 8:+70943954 MsaT045294.1:CDS
ATCTTGAACCCCTCAAGTAA+TGG 0.568276 8:+70944497 MsaT045294.1:CDS
AGAGTGTCACGTTCTTTAGG+AGG 0.573587 8:+70943846 MsaT045294.1:CDS
CAATTTGGAAGCCATAATCA+TGG 0.577888 8:+70944266 MsaT045294.1:CDS
TCATATAGCTGAATTGCTGT+TGG 0.578184 8:-70943936 None:intergenic
AATGAAAGAACCATTACTTG+AGG 0.584582 8:-70944507 None:intergenic
ACAAAACAAGAAGGTGAACA+TGG 0.590894 8:+70943741 MsaT045294.1:CDS
AAAGAAGCAACAAAACAAGA+AGG 0.599775 8:+70943732 MsaT045294.1:CDS
GTACAGACAACCTAATCCTA+CGG 0.604529 8:-70943914 None:intergenic
TCTTATCAAATAAGTACTAG+TGG 0.608228 8:+70944302 MsaT045294.1:CDS
TGAAAGAACCATTACTTGAG+GGG 0.615132 8:-70944505 None:intergenic
GATGGCAATGGCATAACAGA+AGG 0.624451 8:-70944401 None:intergenic
TGGAACTAAACAGTGTTGGA+TGG 0.631286 8:-70944568 None:intergenic
AAACTTTGAGGAATATCAAG+TGG 0.636206 8:-70944047 None:intergenic
CAATAGCTACATCAAGACAA+TGG 0.639730 8:+70943796 MsaT045294.1:CDS
CTTTGAGGAATATCAAGTGG+TGG 0.640320 8:-70944044 None:intergenic
CAAACCCTATTTCAGCAACC+AGG 0.642234 8:+70944080 MsaT045294.1:CDS
TTAGAGGATTAAGAGACCGT+AGG 0.643012 8:+70943898 MsaT045294.1:CDS
AGGGGTTCAAGATTTGAACA+TGG 0.658690 8:-70944487 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 70943702 70944631 70943702 ID=MsaG045294
Chr8 mRNA 70943702 70944631 70943702 ID=MsaT045294.1;Parent=MsaG045294
Chr8 exon 70943702 70944631 70943702 ID=MsaT045294.1.exon1;Parent=MsaT045294.1
Chr8 CDS 70943702 70944631 70943702 ID=cds.MsaT045294.1;Parent=MsaT045294.1
Gene Sequence

>MsaT045294.1

ATGATAGAAAGTTCAAGTAAAGATTATAACAAAGAAGCAACAAAACAAGAAGGTGAACATGGAAGAATGGAATCAGAGAAATTCTCCAACAAAGCAATAGCTACATCAAGACAATGGACATCATTAAGAAATCCAAGAATAGTGAGAGTGTCACGTTCTTTAGGAGGAAAAGACAGACACAGCAAAGTTTGCACAATTAGAGGATTAAGAGACCGTAGGATTAGGTTGTCTGTACCAACAGCAATTCAGCTATATGATCTTCAAGACAGACTTGGTTTAAGTCAACCTAGCAAAGTTGTTGAATGGTTAATTGAAGCAACTCAATCTGATATTGATAAATTACCACCACTTGATATTCCTCAAAGTTTCACTCAACAACAAACCCTATTTCAGCAACCAGGTAGTACTTGTTTTTCTCTTGGAGGTGGTTTTTATGGTATGAATCAAACTAAAGGTAAAGAAGCTGAAAGTTCAGAAAAGGGTAATTATTATAATACTAATTATTCATCAAATGGTTTTCCTTTTCCATATAAATTGGATCCTTCAAGTACTCTATCTTTATCACAATTTGGAAGCCATAATCATGGTAATTTATTTCCTTCTTATCAAATAAGTACTAGTGGCAGTGATATGCAATTTTCATCATCTTCAAATTTGACAGTACCTAATTCATCTGGTAATTCACAGTTACTTTTGTGTCCTTCTGTTATGCCATTGCCATCACTTTTTACTAGTACTCTGAATGCTCCATCAATGGAAAGTAGTGAATTTCCAAGACAATCCAACCATGTTCAAATCTTGAACCCCTCAAGTAATGGTTCTTTCATTCCAACTATTCATCTTCCAATAAATTCGCCGTTTAGACGCCATCCAACACTGTTTAGTTCCAAGCTTCTTGATTCAGATGATAATAACACCAGAAATGGGTAG

Protein sequence

>MsaT045294.1

MIESSSKDYNKEATKQEGEHGRMESEKFSNKAIATSRQWTSLRNPRIVRVSRSLGGKDRHSKVCTIRGLRDRRIRLSVPTAIQLYDLQDRLGLSQPSKVVEWLIEATQSDIDKLPPLDIPQSFTQQQTLFQQPGSTCFSLGGGFYGMNQTKGKEAESSEKGNYYNTNYSSNGFPFPYKLDPSSTLSLSQFGSHNHGNLFPSYQISTSGSDMQFSSSSNLTVPNSSGNSQLLLCPSVMPLPSLFTSTLNAPSMESSEFPRQSNHVQILNPSSNGSFIPTIHLPINSPFRRHPTLFSSKLLDSDDNNTRNG*