AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045299 MtrunA17_Chr4g0061651 96.383 470 17 0 1 470 1 470 0.0 939
MsaG045299 MtrunA17_Chr2g0308321 62.272 493 147 11 12 470 52 539 9.93e-173 496
MsaG045299 MtrunA17_Chr5g0420051 49.315 219 88 8 56 264 111 316 3.24e-51 179
MsaG045299 MtrunA17_Chr3g0132091 54.348 138 42 4 75 198 18 148 2.42e-38 142
MsaG045299 MtrunA17_Chr7g0224821 35.331 317 179 8 75 382 26 325 6.09e-38 142
MsaG045299 MtrunA17_Chr8g0386271 38.194 288 132 11 75 339 45 309 8.32e-37 139
MsaG045299 MtrunA17_Chr7g0231911 75.676 74 18 0 123 196 149 222 5.53e-34 130
MsaG045299 MtrunA17_Chr3g0122411 50.769 130 57 2 72 198 115 240 6.54e-34 132
MsaG045299 MtrunA17_Chr7g0258471 69.136 81 25 0 117 197 136 216 1.95e-33 130
MsaG045299 MtrunA17_Chr8g0334671 73.684 76 20 0 123 198 187 262 3.50e-33 128
MsaG045299 MtrunA17_Chr4g0046991 72.973 74 20 0 123 196 73 146 4.47e-33 122
MsaG045299 MtrunA17_Chr2g0282521 70.886 79 23 0 118 196 59 137 2.49e-32 120
MsaG045299 MtrunA17_Chr8g0360361 70.270 74 22 0 123 196 65 138 2.18e-31 119
MsaG045299 MtrunA17_Chr2g0302791 45.946 148 69 3 58 196 67 212 1.86e-30 125
MsaG045299 MtrunA17_Chr7g0270541 69.737 76 23 0 121 196 143 218 2.03e-30 125
MsaG045299 MtrunA17_Chr1g0162871 62.366 93 31 1 110 198 87 179 1.18e-29 123
MsaG045299 MtrunA17_Chr1g0194121 69.737 76 23 0 121 196 145 220 1.43e-29 122
MsaG045299 MtrunA17_Chr8g0377421 57.471 87 35 1 122 206 131 217 1.50e-27 112
MsaG045299 MtrunA17_Chr8g0377401 61.644 73 28 0 123 195 162 234 4.13e-25 103
MsaG045299 MtrunA17_Chr2g0286661 52.703 74 35 0 123 196 103 176 7.66e-21 95.9
MsaG045299 MtrunA17_Chr1g0175031 68.085 47 15 0 123 169 74 120 1.77e-15 73.2
MsaG045299 MtrunA17_Chr2g0317041 65.957 47 16 0 123 169 99 145 1.86e-15 73.9
MsaG045299 MtrunA17_Chr8g0377431 55.556 54 24 0 123 176 151 204 8.66e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045299 AT1G69170.1 64.286 126 32 3 75 197 83 198 9.01e-45 162
MsaG045299 AT5G50570.1 46.067 178 84 4 123 288 101 278 2.54e-37 140
MsaG045299 AT5G50570.2 46.067 178 84 4 123 288 101 278 2.54e-37 140
MsaG045299 AT5G50670.1 46.067 178 84 4 123 288 101 278 2.54e-37 140
MsaG045299 AT5G50670.2 46.067 178 84 4 123 288 101 278 2.54e-37 140
MsaG045299 AT1G27370.6 68.966 87 27 0 123 209 176 262 2.34e-36 137
MsaG045299 AT1G27370.1 68.966 87 27 0 123 209 176 262 6.29e-36 137
MsaG045299 AT1G27370.5 68.966 87 27 0 123 209 176 262 6.29e-36 137
MsaG045299 AT1G27370.3 68.966 87 27 0 123 209 176 262 6.29e-36 137
MsaG045299 AT1G27370.2 68.966 87 27 0 123 209 176 262 6.29e-36 137
MsaG045299 AT1G27370.4 68.966 87 27 0 123 209 176 262 6.29e-36 137
MsaG045299 AT1G27370.7 68.966 87 27 0 123 209 176 262 6.29e-36 137
MsaG045299 AT1G27360.2 46.429 168 65 4 53 209 108 261 4.81e-35 135
MsaG045299 AT1G27360.1 46.429 168 65 4 53 209 108 261 4.81e-35 135
MsaG045299 AT1G27360.3 46.429 168 65 4 53 209 108 261 4.81e-35 135
MsaG045299 AT1G27360.4 46.429 168 65 4 53 209 108 261 4.81e-35 135
MsaG045299 AT5G43270.3 46.199 171 68 6 48 209 94 249 7.24e-34 132
MsaG045299 AT5G43270.1 46.199 171 68 6 48 209 94 249 7.24e-34 132
MsaG045299 AT5G43270.2 46.199 171 68 6 48 209 94 249 7.24e-34 132
MsaG045299 AT2G33810.1 67.089 79 26 0 118 196 49 127 6.19e-33 122
MsaG045299 AT3G57920.1 70.588 85 25 0 114 198 50 134 1.34e-32 127
MsaG045299 AT2G42200.1 75.000 76 19 0 123 198 74 149 1.81e-32 127
MsaG045299 AT1G02065.1 72.727 77 21 0 120 196 185 261 2.08e-32 126
MsaG045299 AT2G47070.1 73.684 76 20 0 121 196 104 179 2.81e-31 128
MsaG045299 AT3G60030.1 72.973 74 20 0 123 196 127 200 4.79e-31 127
MsaG045299 AT1G53160.2 51.667 120 48 3 123 232 54 173 5.03e-31 118
MsaG045299 AT1G53160.1 51.667 120 48 3 123 232 54 173 5.03e-31 118
MsaG045299 AT1G20980.1 62.000 100 35 1 110 206 104 203 8.55e-31 127
MsaG045299 AT1G76580.2 72.368 76 21 0 123 198 114 189 3.32e-30 124
MsaG045299 AT1G76580.3 72.368 76 21 0 123 198 114 189 3.49e-30 124
MsaG045299 AT1G53160.3 62.069 87 31 1 123 207 54 140 4.43e-30 115
MsaG045299 AT1G76580.1 72.368 76 21 0 123 198 114 189 6.71e-30 124
MsaG045299 AT3G15270.1 63.636 77 28 0 123 199 63 139 1.04e-28 112
MsaG045299 AT5G18830.2 54.023 87 39 1 112 197 127 213 2.09e-22 101
MsaG045299 AT5G18830.3 54.023 87 39 1 112 197 127 213 2.24e-22 101
MsaG045299 AT5G18830.1 54.023 87 39 1 112 197 127 213 2.35e-22 100
MsaG045299 AT2G47070.2 76.000 50 12 0 147 196 1 50 5.05e-18 87.4
MsaG045299 AT1G02065.2 64.706 51 18 0 120 170 185 235 2.49e-16 79.0

Find 97 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCATATTGTCAAGTTTA+TGG 0.148069 8:-71009171 MsaT045299.1:CDS
AGGTCTTCCAAAGTGGTTTC+TGG 0.202370 8:-71007658 MsaT045299.1:CDS
GGCGTTCACTCTACAAATTC+TGG 0.217076 8:-71008236 MsaT045299.1:CDS
CTGCTGGTGGCTTGGTTCCC+TGG 0.251209 8:+71007699 None:intergenic
GGACTGGCTTGAAGTGTTAT+TGG 0.259748 8:+71007328 None:intergenic
AGTTATATTTCTGAAGAAAA+AGG 0.280021 8:-71009516 MsaT045299.1:CDS
CACAAACTTTATGCCTCTTA+TGG 0.303462 8:+71009115 None:intergenic
CAAAATAGGTGCTGCTCCTT+TGG 0.306496 8:+71009245 None:intergenic
CCAAATTACTTTAAGTAATC+CGG 0.320661 8:+71006949 None:intergenic
GGGAGACTGTTTCAGCCATT+TGG 0.327396 8:-71008215 MsaT045299.1:CDS
TATGGCCTGCAAGTCGTTTA+CGG 0.342741 8:+71008282 None:intergenic
TCAGATGATTTCAATTTCAT+TGG 0.348900 8:-71009450 MsaT045299.1:CDS
CCAAGCATTCAAGGATTATC+AGG 0.351371 8:-71007392 MsaT045299.1:CDS
GCTTCATGTTCTGCTTTCAT+AGG 0.354620 8:+71007632 None:intergenic
TCCATCTGTAGGATTTAATT+CGG 0.357577 8:+71007196 None:intergenic
TTATTGTCTGGTACCAATAT+AGG 0.364521 8:+71007161 None:intergenic
GTTCCAAGCAAGAAACTTGA+TGG 0.403736 8:-71009423 MsaT045299.1:CDS
TGAGTGCACTCTCACTCTCT+TGG 0.404810 8:+71009197 None:intergenic
CATACCGCACCAAGCATTCA+AGG 0.405157 8:-71007401 MsaT045299.1:CDS
CAAGGATCTTAGTTCTTGTA+AGG 0.406648 8:-71009142 MsaT045299.1:CDS
TGATATCGTGAACTTTGAAA+TGG 0.410198 8:-71007138 MsaT045299.1:CDS
GACTCACAAGAAGACAAAAT+AGG 0.410388 8:+71009231 None:intergenic
TCTTGCAGATATCAGATTCC+AGG 0.416127 8:-71007717 MsaT045299.1:intron
AATTCCCTTTGATAACCCTT+TGG 0.423487 8:-71007300 MsaT045299.1:CDS
CAGAACATGAAGCTGGCTTT+AGG 0.424799 8:-71007622 MsaT045299.1:CDS
TGTTGCATGCAATGGAATCT+TGG 0.426426 8:-71009539 None:intergenic
GAGTGCACTCTCACTCTCTT+GGG 0.430597 8:+71009198 None:intergenic
TTACCATCAAGTTTCTTGCT+TGG 0.431934 8:+71009420 None:intergenic
AGTTGCCGTAAACGACTTGC+AGG 0.435658 8:-71008287 MsaT045299.1:CDS
CCTGATAATCCTTGAATGCT+TGG 0.438418 8:+71007392 None:intergenic
AAGTCATCTCAGTCCTATAT+TGG 0.441537 8:-71007174 MsaT045299.1:CDS
ATTCCATTCACAATGACTAT+AGG 0.443748 8:+71009075 None:intergenic
CAAAGGGAATTTCCAAGGAC+TGG 0.449402 8:+71007312 None:intergenic
TTGATTCTTCTTGCACTCTC+CGG 0.459359 8:-71006968 MsaT045299.1:CDS
TCTCTCATTGATTTGAAGCT+AGG 0.463051 8:-71009312 MsaT045299.1:CDS
CACAATGACTATAGGAGTCT+TGG 0.465991 8:+71009083 None:intergenic
TAACACTTCAAGCCAGTCCT+TGG 0.468157 8:-71007324 MsaT045299.1:CDS
TCTTCTGTGCCTGTCACCAA+TGG 0.476821 8:-71007593 MsaT045299.1:CDS
GCATCATAGACAATCATTGC+AGG 0.480777 8:-71007003 MsaT045299.1:CDS
GCGTTCACTCTACAAATTCT+GGG 0.481125 8:-71008235 MsaT045299.1:CDS
TTCACGATATCATTATTGTC+TGG 0.484373 8:+71007149 None:intergenic
CATTCACAAAATCTGAATCA+TGG 0.487792 8:+71007102 None:intergenic
CTAGGAAGATTTGATGATCA+TGG 0.493709 8:-71009294 MsaT045299.1:CDS
GCCATTGGCTACACCTCCTT+AGG 0.499701 8:-71007446 MsaT045299.1:CDS
ATTGAAGTCTTCGCTTCCTA+AGG 0.501723 8:+71007430 None:intergenic
TATATATTATTACCACCAAA+TGG 0.502996 8:+71008200 None:intergenic
AAAAGATGCTGCTGGTGGCT+TGG 0.511710 8:+71007691 None:intergenic
TAGACATGGCTATGGCTGCT+TGG 0.512721 8:+71007272 None:intergenic
TGTGGTGGAATCCAATGGTT+GGG 0.513935 8:-71009337 MsaT045299.1:CDS
ACTCCTATAGTCATTGTGAA+TGG 0.516933 8:-71009078 MsaT045299.1:CDS
TTGCTGTCTGAGTTTGATGA+TGG 0.518570 8:-71008317 MsaT045299.1:CDS
TCCGAATTAAATCCTACAGA+TGG 0.523648 8:-71007197 MsaT045299.1:CDS
AATGAGAGAATCCCAACCAT+TGG 0.523865 8:+71009326 None:intergenic
AAACCACTTTGGAAGACCTC+TGG 0.529651 8:+71007662 None:intergenic
AGCCATTATGCTCATGCCAT+TGG 0.530681 8:-71007461 MsaT045299.1:CDS
AGCCAATGGCATGAGCATAA+TGG 0.532501 8:+71007459 None:intergenic
TAGGTCTCCAGAAACCACTT+TGG 0.532795 8:+71007651 None:intergenic
CTTGTAAGGACTACCATAAG+AGG 0.534199 8:-71009128 MsaT045299.1:CDS
TTTCAAGCTTTCAAATTCTG+TGG 0.537070 8:-71009355 MsaT045299.1:CDS
TTGCATCATCTTCACAAGAA+AGG 0.541164 8:+71007069 None:intergenic
TTATGCTGTAGACATGGCTA+TGG 0.541912 8:+71007264 None:intergenic
AATGTTGCCACATCCACTAC+AGG 0.543311 8:-71007506 MsaT045299.1:CDS
ATAACCAAAGGGTTATCAAA+GGG 0.544827 8:+71007296 None:intergenic
CAAGCTTTCAAATTCTGTGG+TGG 0.545205 8:-71009352 MsaT045299.1:CDS
GTTATCAAAGGGAATTTCCA+AGG 0.547080 8:+71007307 None:intergenic
AGACTGTTTCAGCCATTTGG+TGG 0.551343 8:-71008212 MsaT045299.1:intron
GGATAGAGAAAAGATGCTGC+TGG 0.557359 8:+71007683 None:intergenic
AATAACCAAAGGGTTATCAA+AGG 0.561243 8:+71007295 None:intergenic
TGGCTGCTTGGAATAACCAA+AGG 0.562021 8:+71007284 None:intergenic
CTGTGGTGGAATCCAATGGT+TGG 0.564108 8:-71009338 MsaT045299.1:CDS
ATGAAAGCAGAACATGAAGC+TGG 0.566946 8:-71007629 MsaT045299.1:CDS
GGCTGCTTGGAATAACCAAA+GGG 0.567623 8:+71007285 None:intergenic
GAAGTCTTCGCTTCCTAAGG+AGG 0.569996 8:+71007433 None:intergenic
GTGAACTTTGAAATGGAAGA+CGG 0.572384 8:-71007131 MsaT045299.1:CDS
CTTACTGCCTGTAGTGGATG+TGG 0.574967 8:+71007499 None:intergenic
TGAACTTTGAAATGGAAGAC+GGG 0.575100 8:-71007130 MsaT045299.1:CDS
ACTGAACTTACTGCCTGTAG+TGG 0.586429 8:+71007493 None:intergenic
CTGAGATGACTTCCATCTGT+AGG 0.587562 8:+71007185 None:intergenic
TCTTGATTGAAGATGTCCAT+TGG 0.588804 8:+71007577 None:intergenic
TCTATCAGACACTCGGCTTG+AGG 0.591247 8:+71007226 None:intergenic
TGAAGATGTCCATTGGTGAC+AGG 0.591626 8:+71007584 None:intergenic
CTTGCAGATATCAGATTCCA+GGG 0.591867 8:-71007716 MsaT045299.1:intron
TGCACTCTCACTCTCTTGGG+AGG 0.598164 8:+71009201 None:intergenic
TCCTAAGGAGGTGTAGCCAA+TGG 0.598734 8:+71007445 None:intergenic
TATCCAGAGGTCTTCCAAAG+TGG 0.601895 8:-71007665 MsaT045299.1:CDS
TTGTGAATGGAATTGAGCAG+AGG 0.621516 8:-71009065 MsaT045299.1:CDS
AATTCTGTGGTGGAATCCAA+TGG 0.627306 8:-71009342 MsaT045299.1:CDS
TGGCAATGCTTCAATTGATG+CGG 0.627501 8:-71009274 MsaT045299.1:CDS
TAAGAGGCATAAAGTTTGTG+AGG 0.628037 8:-71009112 MsaT045299.1:CDS
CGACAGTTGTAGTAAATCGA+TGG 0.628450 8:+71007046 None:intergenic
GTGATCTTATGCTGTAGACA+TGG 0.639068 8:+71007258 None:intergenic
ATAAAAGTCTATCAGACACT+CGG 0.644463 8:+71007219 None:intergenic
TCAAGTTTATGGATGTAACA+AGG 0.656151 8:-71009160 MsaT045299.1:CDS
TTTGTAGAGTGAACGCCTTG+TGG 0.665425 8:+71008242 None:intergenic
TAGAGAAAAGATGCTGCTGG+TGG 0.692709 8:+71007686 None:intergenic
TAATCCTTGAATGCTTGGTG+CGG 0.726498 8:+71007397 None:intergenic
GAGCGTCGAAGAAAACCACA+AGG 0.736819 8:-71008257 MsaT045299.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 71006959 71009550 71006959 ID=MsaG045299
Chr8 mRNA 71006959 71009550 71006959 ID=MsaT045299.1;Parent=MsaG045299
Chr8 exon 71006959 71007731 71006959 ID=MsaT045299.1.exon3;Parent=MsaT045299.1
Chr8 CDS 71006959 71007731 71006959 ID=cds.MsaT045299.1;Parent=MsaT045299.1
Chr8 exon 71008213 71008346 71008213 ID=MsaT045299.1.exon2;Parent=MsaT045299.1
Chr8 CDS 71008213 71008346 71008213 ID=cds.MsaT045299.1;Parent=MsaT045299.1
Chr8 exon 71009045 71009550 71009045 ID=MsaT045299.1.exon1;Parent=MsaT045299.1
Chr8 CDS 71009045 71009550 71009045 ID=cds.MsaT045299.1;Parent=MsaT045299.1
Gene Sequence

>MsaT045299.1

ATGGAATCTTGGAGTTATATTTCTGAAGAAAAAGGTTCTGAAGAATTAGGCTTTTGTGAAATGTTTGGGAAACAATATTCAGATGATTTCAATTTCATTGGAAGTGTTCCAAGCAAGAAACTTGATGGTAAATTTGATGACCCAAGTGAGTTTTCTGTAAAAGGGGACACAAATTTCAAGCTTTCAAATTCTGTGGTGGAATCCAATGGTTGGGATTCTCTCATTGATTTGAAGCTAGGAAGATTTGATGATCATGGCAATGCTTCAATTGATGCGGCATTTTCCAAAGGAGCAGCACCTATTTTGTCTTCTTGTGAGTCTTCAACACCTCCCAAGAGAGTGAGAGTGCACTCAATGACTGCATATTGTCAAGTTTATGGATGTAACAAGGATCTTAGTTCTTGTAAGGACTACCATAAGAGGCATAAAGTTTGTGAGGTTCACTCCAAGACTCCTATAGTCATTGTGAATGGAATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTTCATTTGCTGTCTGAGTTTGATGATGGTAAGCGCAGTTGCCGTAAACGACTTGCAGGCCATAATGAGCGTCGAAGAAAACCACAAGGCGTTCACTCTACAAATTCTGGGAGACTGTTTCAGCCATTTGGTGATATCAGATTCCAGGGAACCAAGCCACCAGCAGCATCTTTTCTCTATCCAGAGGTCTTCCAAAGTGGTTTCTGGAGACCTATGAAAGCAGAACATGAAGCTGGCTTTAGGCATTTTTCTTCTGTGCCTGTCACCAATGGACATCTTCAATCAAGATCTCCGTTTCCTTCGTACAACGAAAAACAATTTCCCTTTCTTCATGAAAATGTTGCCACATCCACTACAGGCAGTAAGTTCAGTGAAAACAATAGCCATTATGCTCATGCCATTGGCTACACCTCCTTAGGAAGCGAAGACTTCAATGTTTTTCATACCGCACCAAGCATTCAAGGATTATCAGGAATTTCAGACTCTTGTGCTCTCTCTCTTCTGTCATCTCAGTCCAATAACACTTCAAGCCAGTCCTTGGAAATTCCCTTTGATAACCCTTTGGTTATTCCAAGCAGCCATAGCCATGTCTACAGCATAAGATCACAAGCGACTTCCTCAAGCCGAGTGTCTGATAGACTTTTATCCGAATTAAATCCTACAGATGGAAGTCATCTCAGTCCTATATTGGTACCAGACAATAATGATATCGTGAACTTTGAAATGGAAGACGGGATCTTCCATGATTCAGATTTTGTGAATGTGAAAGACTGCCTTTCTTGTGAAGATGATGCAACCATCGATTTACTACAACTGTCGTCGCAGCTTCAACGAGTTGAGCATCATAGACAATCATTGCAGGTGAAGAAAGAGTTTGATTCTTCTTGCACTCTCCGGATTACTTAA
Protein sequence

>MsaT045299.1

MESWSYISEEKGSEELGFCEMFGKQYSDDFNFIGSVPSKKLDGKFDDPSEFSVKGDTNFKLSNSVVESNGWDSLIDLKLGRFDDHGNASIDAAFSKGAAPILSSCESSTPPKRVRVHSMTAYCQVYGCNKDLSSCKDYHKRHKVCEVHSKTPIVIVNGIEQRFCQQCSRFHLLSEFDDGKRSCRKRLAGHNERRRKPQGVHSTNSGRLFQPFGDIRFQGTKPPAASFLYPEVFQSGFWRPMKAEHEAGFRHFSSVPVTNGHLQSRSPFPSYNEKQFPFLHENVATSTTGSKFSENNSHYAHAIGYTSLGSEDFNVFHTAPSIQGLSGISDSCALSLLSSQSNNTSSQSLEIPFDNPLVIPSSHSHVYSIRSQATSSSRVSDRLLSELNPTDGSHLSPILVPDNNDIVNFEMEDGIFHDSDFVNVKDCLSCEDDATIDLLQLSSQLQRVEHHRQSLQVKKEFDSSCTLRIT*