AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045310 MtrunA17_Chr4g0061761 97.516 322 6 1 1 322 1 320 0.0 643
MsaG045310 MtrunA17_Chr2g0308011 63.000 100 37 0 40 139 37 136 3.61e-34 124
MsaG045310 MtrunA17_Chr2g0328021 63.043 92 25 1 40 131 62 144 5.78e-34 125
MsaG045310 MtrunA17_Chr4g0047001 59.048 105 41 2 20 123 34 137 1.74e-33 127
MsaG045310 MtrunA17_Chr8g0374251 82.759 58 10 0 36 93 64 121 1.28e-31 121
MsaG045310 MtrunA17_Chr8g0341671 80.000 65 12 1 37 100 17 81 1.71e-31 117
MsaG045310 MtrunA17_Chr4g0031411 67.606 71 23 0 37 107 72 142 2.85e-31 120
MsaG045310 MtrunA17_Chr4g0010151 40.361 166 78 3 19 170 54 212 1.16e-30 119
MsaG045310 MtrunA17_Chr7g0237921 47.581 124 47 2 27 150 63 168 1.98e-30 117
MsaG045310 MtrunA17_Chr8g0374111 60.759 79 31 0 37 115 26 104 6.13e-30 114
MsaG045310 MtrunA17_Chr8g0367161 70.833 72 18 1 39 107 31 102 6.63e-30 116
MsaG045310 MtrunA17_Chr7g0223311 82.456 57 10 0 41 97 78 134 1.14e-29 116
MsaG045310 MtrunA17_Chr5g0411951 83.333 54 9 0 37 90 40 93 1.51e-29 115
MsaG045310 MtrunA17_Chr3g0127201 64.865 74 20 1 23 96 16 83 1.69e-29 114
MsaG045310 MtrunA17_Chr4g0030051 70.833 72 15 1 20 91 27 92 2.55e-29 113
MsaG045310 MtrunA17_Chr3g0117601 56.000 100 38 1 23 122 50 143 5.84e-29 113
MsaG045310 MtrunA17_Chr2g0326001 66.667 81 24 1 37 114 37 117 6.19e-29 112
MsaG045310 MtrunA17_Chr2g0282441 86.275 51 7 0 41 91 26 76 2.14e-28 112
MsaG045310 MtrunA17_Chr8g0346551 53.191 94 36 1 21 114 13 98 3.64e-28 110
MsaG045310 MtrunA17_Chr3g0126201 74.194 62 16 0 42 103 53 114 5.83e-28 110
MsaG045310 MtrunA17_Chr1g0177251 77.966 59 13 0 36 94 8 66 3.81e-27 107
MsaG045310 MtrunA17_Chr1g0188851 72.414 58 16 0 37 94 40 97 6.34e-27 107
MsaG045310 MtrunA17_Chr2g0282051 70.769 65 18 1 30 94 36 99 2.56e-26 105
MsaG045310 MtrunA17_Chr8g0355261 68.966 58 18 0 38 95 116 173 3.04e-26 107
MsaG045310 MtrunA17_Chr7g0217581 71.186 59 17 0 38 96 230 288 3.67e-26 108
MsaG045310 MtrunA17_Chr2g0282511 84.000 50 8 0 39 88 44 93 4.68e-26 105
MsaG045310 MtrunA17_Chr2g0283811 68.421 57 18 0 37 93 44 100 9.10e-26 100
MsaG045310 MtrunA17_Chr7g0254161 46.316 95 51 0 38 132 99 193 1.15e-25 106
MsaG045310 MtrunA17_Chr6g0453321 67.797 59 19 0 38 96 134 192 2.08e-25 105
MsaG045310 MtrunA17_Chr4g0042481 67.857 56 18 0 38 93 120 175 1.51e-24 103
MsaG045310 MtrunA17_Chr3g0092111 58.904 73 23 1 31 96 120 192 2.74e-24 102
MsaG045310 MtrunA17_Chr6g0460731 46.000 100 50 1 38 137 94 189 4.84e-24 100
MsaG045310 MtrunA17_Chr5g0417571 67.797 59 19 0 38 96 136 194 4.91e-24 100
MsaG045310 MtrunA17_Chr6g0460721 63.158 57 21 0 38 94 87 143 2.79e-23 98.2
MsaG045310 MtrunA17_Chr5g0417601 63.077 65 23 1 30 93 129 193 3.75e-23 98.6
MsaG045310 MtrunA17_Chr5g0417551 66.102 59 20 0 38 96 125 183 6.08e-23 97.8
MsaG045310 MtrunA17_Chr5g0417561 66.102 59 20 0 38 96 117 175 1.12e-22 97.1
MsaG045310 MtrunA17_Chr4g0047761 35.714 168 79 3 38 203 70 210 6.01e-16 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045310 AT5G60850.1 42.857 322 114 12 23 322 34 307 5.70e-55 181
MsaG045310 AT1G51700.1 76.712 73 16 1 37 108 30 102 2.13e-33 122
MsaG045310 AT5G65590.1 75.758 66 16 0 36 101 37 102 2.06e-32 122
MsaG045310 AT5G60200.1 67.949 78 19 1 39 116 54 125 3.45e-32 120
MsaG045310 AT5G66940.1 56.989 93 34 1 36 122 28 120 1.90e-31 118
MsaG045310 AT3G45610.1 66.234 77 19 1 37 113 37 106 4.52e-31 117
MsaG045310 AT2G37590.1 68.116 69 22 0 27 95 76 144 1.10e-30 118
MsaG045310 AT2G28510.1 42.778 180 79 5 35 202 43 210 3.40e-30 116
MsaG045310 AT1G07640.1 79.310 58 12 0 40 97 21 78 4.08e-30 115
MsaG045310 AT1G07640.2 79.310 58 12 0 40 97 77 134 5.47e-30 116
MsaG045310 AT1G07640.3 79.310 58 12 0 40 97 85 142 6.91e-30 116
MsaG045310 AT2G28810.2 83.019 53 9 0 39 91 76 128 1.45e-29 115
MsaG045310 AT3G52440.1 71.053 76 17 2 27 102 14 84 1.50e-29 113
MsaG045310 AT3G61850.2 65.000 80 20 1 24 103 54 125 1.54e-29 114
MsaG045310 AT3G61850.3 65.000 80 20 1 24 103 54 125 1.54e-29 114
MsaG045310 AT4G38000.1 63.750 80 20 2 20 90 12 91 1.59e-29 113
MsaG045310 AT3G61850.1 65.000 80 20 1 24 103 66 137 1.60e-29 114
MsaG045310 AT3G61850.5 65.000 80 20 1 24 103 54 125 2.12e-29 114
MsaG045310 AT2G28810.1 83.019 53 9 0 39 91 93 145 2.17e-29 115
MsaG045310 AT3G52440.2 71.053 76 17 2 27 102 33 103 2.20e-29 113
MsaG045310 AT3G61850.4 65.000 80 20 1 24 103 66 137 2.49e-29 114
MsaG045310 AT3G21270.1 88.679 53 6 0 40 92 29 81 3.54e-29 111
MsaG045310 AT3G50410.1 77.049 61 14 0 37 97 27 87 3.75e-29 112
MsaG045310 AT3G55370.1 69.118 68 21 0 35 102 71 138 5.23e-29 114
MsaG045310 AT3G55370.2 69.118 68 21 0 35 102 71 138 6.14e-29 114
MsaG045310 AT3G55370.3 69.118 68 21 0 35 102 116 183 8.90e-29 114
MsaG045310 AT1G28310.1 77.966 59 13 0 40 98 27 85 9.44e-29 113
MsaG045310 AT5G02460.1 75.000 60 15 0 35 94 90 149 1.05e-28 114
MsaG045310 AT4G21050.1 55.882 102 38 2 42 137 26 126 1.08e-28 110
MsaG045310 AT1G28310.2 77.966 59 13 0 40 98 51 109 1.54e-28 112
MsaG045310 AT4G24060.1 63.514 74 21 1 21 94 40 107 3.76e-28 112
MsaG045310 AT1G21340.1 80.357 56 11 0 42 97 40 95 8.39e-28 109
MsaG045310 AT5G62940.1 69.565 69 15 1 22 90 61 123 9.50e-28 111
MsaG045310 AT4G00940.1 64.103 78 28 0 37 114 65 142 3.50e-26 105
MsaG045310 AT4G00940.3 64.103 78 28 0 37 114 65 142 3.50e-26 105
MsaG045310 AT4G00940.2 64.103 78 28 0 37 114 65 142 3.50e-26 105
MsaG045310 AT1G29160.1 71.429 56 16 0 38 93 60 115 1.99e-25 100
MsaG045310 AT2G34140.1 71.429 56 16 0 38 93 56 111 2.24e-25 100
MsaG045310 AT3G47500.1 69.492 59 18 0 38 96 108 166 2.54e-25 105
MsaG045310 AT1G64620.1 61.194 67 20 1 23 89 38 98 4.16e-25 103
MsaG045310 AT2G46590.1 65.152 66 15 1 22 87 58 115 6.66e-25 103
MsaG045310 AT2G46590.2 65.152 66 15 1 22 87 70 127 7.26e-25 103
MsaG045310 AT5G39660.3 69.643 56 17 0 38 93 136 191 1.21e-24 103
MsaG045310 AT5G39660.1 69.643 56 17 0 38 93 136 191 1.21e-24 103
MsaG045310 AT5G39660.2 69.643 56 17 0 38 93 136 191 1.21e-24 103
MsaG045310 AT5G62430.1 64.407 59 21 0 38 96 52 110 3.73e-24 100
MsaG045310 AT1G69570.1 69.643 56 17 0 38 93 130 185 6.09e-24 101
MsaG045310 AT1G47655.1 74.510 51 13 0 38 88 27 77 6.31e-24 97.4
MsaG045310 AT1G26790.1 66.071 56 19 0 38 93 109 164 4.08e-23 98.6
MsaG045310 AT4G21040.1 42.069 145 67 3 42 169 27 171 4.68e-23 95.9
MsaG045310 AT4G21080.1 68.966 58 17 1 42 98 27 84 1.45e-21 92.0
MsaG045310 AT4G21030.1 55.882 68 27 1 42 109 23 87 5.04e-16 75.5

Find 96 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTAGAGCGTTTGGATTTC+CGG 0.179446 8:+71150546 None:intergenic
TTCTGGTCCTGGATCGTTGC+TGG 0.251043 8:-71150057 MsaT045310.1:CDS
GTTATTGTAGTAACAGAATT+TGG 0.266870 8:+71150664 None:intergenic
TCCCCGTCGGTGGCGGTTGC+CGG 0.275837 8:-71150565 MsaT045310.1:CDS
GGTTCAGGTTATTGTTGTTT+TGG 0.289348 8:+71150726 None:intergenic
GAGGACATTTCATGACTTCT+TGG 0.303639 8:+71150705 None:intergenic
AAAACAAGCAACGGTGGATT+TGG 0.307667 8:-71150002 MsaT045310.1:CDS
GAGACATTGCCGTTTGGTTT+TGG 0.311028 8:-71150257 MsaT045310.1:CDS
CGGTAACGCTCATCAGCTTT+TGG 0.328939 8:+71150159 None:intergenic
ACGGTAATGTTAACGTTAAC+GGG 0.351619 8:-71150214 MsaT045310.1:CDS
CAAAAGCTGATGAGCGTTAC+CGG 0.354847 8:-71150158 MsaT045310.1:CDS
ACACCGTTGATCATGGTTAC+TGG 0.364180 8:-71149920 MsaT045310.1:CDS
GACTTATTCTCCGGCGCCTC+CGG 0.380062 8:+71150473 None:intergenic
GTAACAGAATTTGGTGTTGA+TGG 0.387408 8:+71150673 None:intergenic
TTCTTCTCCGGCGGTGGTGT+TGG 0.390782 8:+71150383 None:intergenic
TAACCATGATCAACGGTGTT+AGG 0.392627 8:+71149924 None:intergenic
AACGGTAATGTTAACGTTAA+CGG 0.394532 8:-71150215 MsaT045310.1:CDS
ATAGGTGCTGGTGCTGGTGG+GGG 0.397991 8:-71150086 MsaT045310.1:CDS
AATATAGGTGCTGGTGCTGG+TGG 0.411942 8:-71150089 MsaT045310.1:CDS
GGCGCCTCCGGTGGAGTTGT+CGG 0.413936 8:+71150485 None:intergenic
TATAGGTGCTGGTGCTGGTG+GGG 0.419125 8:-71150087 MsaT045310.1:CDS
CAACGGTGGATTTGGATCGT+TGG 0.423030 8:-71149994 MsaT045310.1:CDS
ATTTCATGACTTCTTGGTTC+AGG 0.424145 8:+71150711 None:intergenic
ATATAGGTGCTGGTGCTGGT+GGG 0.429106 8:-71150088 MsaT045310.1:CDS
CACCGCAGCGGCGGTGAGGC+TGG 0.433116 8:+71150424 None:intergenic
CGCCACCGACGGGGACGTTA+CGG 0.435705 8:+71150573 None:intergenic
AAACGGCAATGTCTCGTTAG+AGG 0.437377 8:+71150265 None:intergenic
GGTTCCTCCGGCGCCTCCGA+TGG 0.440934 8:+71150793 None:intergenic
GCCTCCGCCACCGAAGAATC+TGG 0.443448 8:+71150772 None:intergenic
AGACGCATTGAAGTTGTTAC+CGG 0.443650 8:-71150114 MsaT045310.1:CDS
GTGGATTTGGATCGTTGGAT+TGG 0.444872 8:-71149989 MsaT045310.1:CDS
CTTCCTAACACCGTTGATCA+TGG 0.449712 8:-71149927 MsaT045310.1:CDS
TCGGTGGCGGAGGCGGTGAT+CGG 0.459343 8:-71150763 MsaT045310.1:CDS
TTCCGGCAACCGCCACCGAC+GGG 0.461760 8:+71150563 None:intergenic
CAGCCTCACCGCCGCTGCGG+TGG 0.464633 8:-71150423 MsaT045310.1:CDS
AGATTCTTCGGTGGCGGAGG+CGG 0.473172 8:-71150770 MsaT045310.1:CDS
TCACACTCAATGGTCTGATC+AGG 0.473669 8:-71149895 MsaT045310.1:CDS
GCCGGAGGAACCAGATTCTT+CGG 0.474219 8:-71150782 MsaT045310.1:CDS
AAGTTGTTACCGGAGAATAT+AGG 0.476958 8:-71150104 MsaT045310.1:CDS
TAACGTTAACATTACCGTTA+CGG 0.477277 8:+71150219 None:intergenic
GATTCACTTGAACAAGGAAC+AGG 0.481996 8:-71150338 MsaT045310.1:CDS
CGTAACGTCCCCGTCGGTGG+CGG 0.482120 8:-71150572 MsaT045310.1:CDS
GGAACTCTCACTGCTGGAAT+GGG 0.482309 8:+71150445 None:intergenic
TGGAACTCTCACTGCTGGAA+TGG 0.493236 8:+71150444 None:intergenic
TCTAACGAGACATTGCCGTT+TGG 0.499608 8:-71150263 MsaT045310.1:CDS
ACCAGATTCTTCGGTGGCGG+AGG 0.500004 8:-71150773 MsaT045310.1:CDS
GACAACTCCACCGGAGGCGC+CGG 0.504458 8:-71150483 MsaT045310.1:CDS
ACCGAAGAATCTGGTTCCTC+CGG 0.507560 8:+71150781 None:intergenic
CCATGGTCCAGCAACGATCC+AGG 0.515665 8:+71150050 None:intergenic
CAACATTCCATCGGAGGCGC+CGG 0.516567 8:-71150800 MsaT045310.1:CDS
GAGGCTGGAACTCTCACTGC+TGG 0.519133 8:+71150439 None:intergenic
TTTCCGGCAACCGCCACCGA+CGG 0.522246 8:+71150562 None:intergenic
TGCCGACGTTACTGGACTAA+AGG 0.527907 8:-71150605 MsaT045310.1:CDS
CTCCGTAACGTCCCCGTCGG+TGG 0.530524 8:-71150575 MsaT045310.1:CDS
TTATTCTCCGGCGCCTCCGG+TGG 0.532938 8:+71150476 None:intergenic
CAGCCACCGCAGCGGCGGTG+AGG 0.533594 8:+71150420 None:intergenic
ATGATGCGTCGTCGTTTCAG+TGG 0.549551 8:-71150187 MsaT045310.1:CDS
TCGTTGGATTGGAATGGTGA+TGG 0.550292 8:-71149978 MsaT045310.1:CDS
CATTCCATCGGAGGCGCCGG+AGG 0.561089 8:-71150797 MsaT045310.1:CDS
TTTGGATCGTTGGATTGGAA+TGG 0.568406 8:-71149984 MsaT045310.1:CDS
TGGGAGTTAGACTTATTCTC+CGG 0.569296 8:+71150464 None:intergenic
TTCAGAACCAACACCACCGC+CGG 0.573448 8:-71150390 MsaT045310.1:CDS
GTTACTGGAATCACACTCAA+TGG 0.574806 8:-71149905 MsaT045310.1:CDS
TTACCGGAGAATATAGGTGC+TGG 0.577924 8:-71150098 MsaT045310.1:CDS
CGGTAAAACAGTGGAATCTG+AGG 0.581795 8:+71150505 None:intergenic
GTCCTCCGTAACGTCCCCGT+CGG 0.588295 8:-71150578 MsaT045310.1:CDS
TGTTTCAGCCACCGCAGCGG+CGG 0.589914 8:+71150415 None:intergenic
GAGAATATAGGTGCTGGTGC+TGG 0.591108 8:-71150092 MsaT045310.1:CDS
TTCCAGCCTCACCGCCGCTG+CGG 0.594936 8:-71150426 MsaT045310.1:CDS
GGAACCAGATTCTTCGGTGG+CGG 0.595461 8:-71150776 MsaT045310.1:CDS
GCACCAGCACCTATATTCTC+CGG 0.599864 8:+71150095 None:intergenic
AGAAGTGACGAGGCTGAGAG+AGG 0.604907 8:+71150636 None:intergenic
GAACAAGGAACAGGTGATTG+TGG 0.605175 8:-71150329 MsaT045310.1:CDS
TCCGGCAACCGCCACCGACG+GGG 0.606442 8:+71150564 None:intergenic
TTACCGATACCAAAACCAAA+CGG 0.611130 8:+71150248 None:intergenic
CTGCAGAGTAAAACAAGCAA+CGG 0.614794 8:-71150011 MsaT045310.1:CDS
CCTGGATCGTTGCTGGACCA+TGG 0.617986 8:-71150050 MsaT045310.1:CDS
GAGAATGATTCACTTGAACA+AGG 0.621864 8:-71150344 MsaT045310.1:CDS
CAGAGTAAAACAAGCAACGG+TGG 0.633363 8:-71150008 MsaT045310.1:CDS
GAGATACAACATTCCATCGG+AGG 0.634291 8:-71150806 MsaT045310.1:CDS
GCAAAGAGAAATCAATACCA+TGG 0.638840 8:+71150033 None:intergenic
AACTGTTTCAGCCACCGCAG+CGG 0.652527 8:+71150412 None:intergenic
ATCGGTAACGATAACCGTAA+CGG 0.654058 8:-71150233 MsaT045310.1:CDS
TAAAACAGTGGAATCTGAGG+AGG 0.660488 8:+71150508 None:intergenic
TTTACCGACAACTCCACCGG+AGG 0.660654 8:-71150489 MsaT045310.1:CDS
CGCCTTTAGTCCAGTAACGT+CGG 0.661444 8:+71150603 None:intergenic
GATGGTGGTGCAGATCATCA+AGG 0.664273 8:-71149960 MsaT045310.1:CDS
CACCGACGGGGACGTTACGG+AGG 0.667570 8:+71150576 None:intergenic
CAGGAGATACAACATTCCAT+CGG 0.667929 8:-71150809 MsaT045310.1:CDS
TGGAGTTGTCGGTAAAACAG+TGG 0.668985 8:+71150496 None:intergenic
GGAGGAACCAGATTCTTCGG+TGG 0.672184 8:-71150779 MsaT045310.1:CDS
ATTCCAGTAACCATGATCAA+CGG 0.674918 8:+71149917 None:intergenic
CCGGAGAAGAAAATCAACAA+AGG 0.680379 8:-71150371 MsaT045310.1:CDS
TTGGATTGGAATGGTGATGG+TGG 0.685796 8:-71149975 MsaT045310.1:CDS
CGACGTTACTGGACTAAAGG+CGG 0.694270 8:-71150602 MsaT045310.1:CDS
GTGTTGATGGAGTCACAGCG+AGG 0.726737 8:+71150686 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 71149866 71150834 71149866 ID=MsaG045310
Chr8 mRNA 71149866 71150834 71149866 ID=MsaT045310.1;Parent=MsaG045310
Chr8 exon 71149866 71150834 71149866 ID=MsaT045310.1.exon1;Parent=MsaT045310.1
Chr8 CDS 71149866 71150834 71149866 ID=cds.MsaT045310.1;Parent=MsaT045310.1
Gene Sequence

>MsaT045310.1

ATGCAGGAGATACAACATTCCATCGGAGGCGCCGGAGGAACCAGATTCTTCGGTGGCGGAGGCGGTGATCGGCGTCTCAGACCCAACCAAAACAACAATAACCTGAACCAAGAAGTCATGAAATGTCCTCGCTGTGACTCCATCAACACCAAATTCTGTTACTACAATAACTACAACCTCTCTCAGCCTCGTCACTTCTGCAAAAACTGCCGACGTTACTGGACTAAAGGCGGTGTCCTCCGTAACGTCCCCGTCGGTGGCGGTTGCCGGAAATCCAAACGCTCTAGCAAACCAAAAAAATCTTCCTCCTCAGATTCCACTGTTTTACCGACAACTCCACCGGAGGCGCCGGAGAATAAGTCTAACTCCCATTCCAGCAGTGAGAGTTCCAGCCTCACCGCCGCTGCGGTGGCTGAAACAGTTTCAGAACCAACACCACCGCCGGAGAAGAAAATCAACAAAGGTTTTGAGAATGATTCACTTGAACAAGGAACAGGTGATTGTGGTATATTCTCAGAGATCGCAACTTTCACAAGCTTGATCACTTCCTCTAACGAGACATTGCCGTTTGGTTTTGGTATCGGTAACGATAACCGTAACGGTAATGTTAACGTTAACGGGTTTAATGATGCGTCGTCGTTTCAGTGGCAAAACCAAAAGCTGATGAGCGTTACCGGCGATCAAAAACATCATGAAGAAGACGCATTGAAGTTGTTACCGGAGAATATAGGTGCTGGTGCTGGTGGGGGTGGTTCTTCTGGTCCTGGATCGTTGCTGGACCATGGTATTGATTTCTCTTTGCTGCAGAGTAAAACAAGCAACGGTGGATTTGGATCGTTGGATTGGAATGGTGATGGTGGTGCAGATCATCAAGGTTTGTTTGATCTTCCTAACACCGTTGATCATGGTTACTGGAATCACACTCAATGGTCTGATCAGGATCATTCTTCTCTCTTTCATCTTCCATGA

Protein sequence

>MsaT045310.1

MQEIQHSIGGAGGTRFFGGGGGDRRLRPNQNNNNLNQEVMKCPRCDSINTKFCYYNNYNLSQPRHFCKNCRRYWTKGGVLRNVPVGGGCRKSKRSSKPKKSSSSDSTVLPTTPPEAPENKSNSHSSSESSSLTAAAVAETVSEPTPPPEKKINKGFENDSLEQGTGDCGIFSEIATFTSLITSSNETLPFGFGIGNDNRNGNVNVNGFNDASSFQWQNQKLMSVTGDQKHHEEDALKLLPENIGAGAGGGGSSGPGSLLDHGIDFSLLQSKTSNGGFGSLDWNGDGGADHQGLFDLPNTVDHGYWNHTQWSDQDHSSLFHLP*