AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045317

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045317 MtrunA17_Chr7g0225661 35.714 126 66 3 79 200 55 169 4.03e-17 76.3
MsaG045317 MtrunA17_Chr8g0341311 36.364 121 62 3 84 200 50 159 5.06e-17 75.9
MsaG045317 MtrunA17_Chr7g0220571 42.342 111 60 1 73 179 52 162 3.99e-16 73.6
MsaG045317 MtrunA17_Chr6g0456061 41.441 111 60 1 76 181 57 167 5.62e-16 73.2
MsaG045317 MtrunA17_Chr4g0035414 35.833 120 64 3 93 199 16 135 6.76e-13 63.9
MsaG045317 MtrunA17_Chr5g0400901 36.232 138 71 6 73 202 27 155 1.57e-12 63.2
MsaG045317 MtrunA17_Chr3g0144941 37.255 102 54 2 93 184 16 117 8.60e-12 61.2
MsaG045317 MtrunA17_Chr1g0194681 30.769 117 74 1 84 193 7 123 4.04e-11 59.3
MsaG045317 MtrunA17_Chr4g0054291 33.333 129 72 4 50 172 23 143 7.14e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045317 AT5G60830.1 54.118 85 39 0 102 186 69 153 4.27e-22 90.1
MsaG045317 AT5G38800.1 41.667 96 54 1 86 179 52 147 2.62e-18 79.0
MsaG045317 AT3G30530.1 41.176 102 60 0 82 183 59 160 7.15e-18 78.2
MsaG045317 AT1G13600.1 42.105 95 50 1 90 179 67 161 1.50e-16 75.1
MsaG045317 AT5G15830.1 36.036 111 65 1 92 196 56 166 1.28e-14 69.7
MsaG045317 AT2G04038.1 40.426 94 50 1 92 179 56 149 8.42e-14 67.0
MsaG045317 AT2G18160.1 34.211 114 68 1 90 196 17 130 4.24e-12 62.8
MsaG045317 AT1G75390.1 34.959 123 65 3 82 189 11 133 3.45e-11 60.1
MsaG045317 AT3G62420.1 33.898 118 69 3 87 196 8 124 4.04e-11 59.3

Find 54 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGTTCATGATGGGTTTC+TGG 0.068192 8:-71299639 None:intergenic
CCAACAAGTAAATGATCTTT+TGG 0.211603 8:-71299996 None:intergenic
ATGAGTTGGGAGAAGATTTA+TGG 0.268120 8:-71299798 None:intergenic
CCAAAAGATCATTTACTTGT+TGG 0.311300 8:+71299996 MsaT045317.1:CDS
GTGAGGTATACCAACATTTC+CGG 0.334411 8:-71300109 None:intergenic
TGAGTTCATGATGGGTTTCT+GGG 0.383025 8:-71299638 None:intergenic
AGCGATACTATCTACTTTGT+TGG 0.386310 8:+71300080 MsaT045317.1:CDS
TTTATGGTCTGGAATTTGAA+TGG 0.416953 8:-71299782 None:intergenic
CGGAAGCATAGGGTACTTCA+TGG 0.433114 8:-71299585 None:intergenic
TTGGGAGAAGATTTATGGTC+TGG 0.437689 8:-71299793 None:intergenic
TTGCAGGCTTGAGTTCATGA+TGG 0.445548 8:-71299647 None:intergenic
GTTACTGTTTGATGATGAGT+TGG 0.446587 8:-71299812 None:intergenic
TGAAGCTGGGAGTAGTGAAA+TGG 0.452683 8:-71299736 None:intergenic
TTATGGTCTGGAATTTGAAT+GGG 0.454968 8:-71299781 None:intergenic
TTCATGATGGGTTTCTGGGT+TGG 0.465026 8:-71299634 None:intergenic
TGCAGGCTTGAGTTCATGAT+GGG 0.472548 8:-71299646 None:intergenic
TTGATGATCGAAAGAAGAAA+AGG 0.480340 8:+71299863 MsaT045317.1:CDS
AGACGAGTTGAAAGGTTGGC+TGG 0.483172 8:-71300150 None:intergenic
TGTCGTTAGAGAGGTGAAGC+TGG 0.483487 8:-71299750 None:intergenic
ACCCATCTGTATCCATCTCA+AGG 0.506932 8:+71299700 MsaT045317.1:CDS
CTACTCAAAATCGCTATGCT+CGG 0.512535 8:-71300193 None:intergenic
TCTACTTTGTTGGTTACGAC+CGG 0.517483 8:+71300090 MsaT045317.1:CDS
GTACTGTATCACGGAAGCAT+AGG 0.517499 8:-71299596 None:intergenic
TACTCAAAATCGCTATGCTC+GGG 0.518651 8:-71300192 None:intergenic
TTACTGTTTGATGATGAGTT+GGG 0.525916 8:-71299811 None:intergenic
ATTGATGGTTCAGCGCCTGC+AGG 0.527456 8:-71299969 None:intergenic
AGGTTGGCTGGGAAGCCACT+AGG 0.529008 8:-71300138 None:intergenic
ATGGGTTTCTGGGTTGGAGA+AGG 0.532216 8:-71299628 None:intergenic
GATACAGATGGGTCAAAGAA+TGG 0.534656 8:-71299690 None:intergenic
CTGGGAAGCCACTAGGGATG+TGG 0.538645 8:-71300131 None:intergenic
TTTCTGGGTTGGAGAAGGAT+AGG 0.544788 8:-71299623 None:intergenic
GTATCAAGTGAATCACCTGC+AGG 0.567327 8:+71299954 MsaT045317.1:CDS
ATATCATGTAAACCTTGAGA+TGG 0.573761 8:-71299712 None:intergenic
TCAAACAGTAACAACAACTC+TGG 0.574952 8:+71299823 MsaT045317.1:CDS
GTCGTTAGAGAGGTGAAGCT+GGG 0.575441 8:-71299749 None:intergenic
GACGAGTTGAAAGGTTGGCT+GGG 0.575767 8:-71300149 None:intergenic
AACCTTGAGATGGATACAGA+TGG 0.576394 8:-71299702 None:intergenic
TACTGTATCACGGAAGCATA+GGG 0.579135 8:-71299595 None:intergenic
GGTTGGCTGGGAAGCCACTA+GGG 0.587937 8:-71300137 None:intergenic
TTGTAACTAGACGAGTTGAA+AGG 0.591340 8:-71300158 None:intergenic
TTGGTTACGACCGGAAATGT+TGG 0.596096 8:+71300099 MsaT045317.1:CDS
GACTGTCGCTGTCGTTAGAG+AGG 0.603830 8:-71299759 None:intergenic
CAACAACTCTGGTAAAGCAA+AGG 0.613348 8:+71299834 MsaT045317.1:CDS
GGTGATTCACTTGATACCAC+AGG 0.615500 8:-71299948 None:intergenic
AACTAGACGAGTTGAAAGGT+TGG 0.615858 8:-71300154 None:intergenic
ACCTTGAGATGGATACAGAT+GGG 0.616103 8:-71299701 None:intergenic
AGATAGTATCGCTTGAAGAG+AGG 0.616741 8:-71300070 None:intergenic
ATAGGAAAGGTACTGTATCA+CGG 0.627119 8:-71299605 None:intergenic
CTAACAGAGAATCTGCTCGT+CGG 0.675721 8:+71299893 MsaT045317.1:CDS
AGAGATGGTACGCAATGACG+AGG 0.676375 8:+71299558 None:intergenic
ATACCTCACCACATCCCTAG+TGG 0.679014 8:+71300123 MsaT045317.1:CDS
AGCAGCGAATTGAAGTCCTG+TGG 0.703639 8:+71299932 MsaT045317.1:CDS
AAGCCACTAGGGATGTGGTG+AGG 0.721984 8:-71300126 None:intergenic
GGGTTGGAGAAGGATAGGAA+AGG 0.730828 8:-71299618 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 71299562 71300215 71299562 ID=MsaG045317
Chr8 mRNA 71299562 71300215 71299562 ID=MsaT045317.1;Parent=MsaG045317
Chr8 exon 71299562 71300215 71299562 ID=MsaT045317.1.exon1;Parent=MsaT045317.1
Chr8 CDS 71299562 71300215 71299562 ID=cds.MsaT045317.1;Parent=MsaT045317.1
Gene Sequence

>MsaT045317.1

ATGGTACGCAATGACGAGGAACTCCATGAAGTACCCTATGCTTCCGTGATACAGTACCTTTCCTATCCTTCTCCAACCCAGAAACCCATCATGAACTCAAGCCTGCAAAACCTGAAAAATGGAGAAGGCCATTCTTTGACCCATCTGTATCCATCTCAAGGTTTACATGATATTCCATTTCACTACTCCCAGCTTCACCTCTCTAACGACAGCGACAGTCCCATTCAAATTCCAGACCATAAATCTTCTCCCAACTCATCATCAAACAGTAACAACAACTCTGGTAAAGCAAAGGATCTCCTTGATGATCGAAAGAAGAAAAGGATGTTCTCTAACAGAGAATCTGCTCGTCGGTCGCGAATGAGAAAGAAGCAGCGAATTGAAGTCCTGTGGTATCAAGTGAATCACCTGCAGGCGCTGAACCATCAATTGTCCCAAAAGATCATTTACTTGTTGGAATGTAACCAACTAATCAACCAACAAAATGCTCAACTTAAAGACAAAGTTTCCTCTCTTCAAGCGATACTATCTACTTTGTTGGTTACGACCGGAAATGTTGGTATACCTCACCACATCCCTAGTGGCTTCCCAGCCAACCTTTCAACTCGTCTAGTTACAAACTTACAATTTCCCGAGCATAGCGATTTTGAGTAG
Protein sequence

>MsaT045317.1

MVRNDEELHEVPYASVIQYLSYPSPTQKPIMNSSLQNLKNGEGHSLTHLYPSQGLHDIPFHYSQLHLSNDSDSPIQIPDHKSSPNSSSNSNNNSGKAKDLLDDRKKKRMFSNRESARRSRMRKKQRIEVLWYQVNHLQALNHQLSQKIIYLLECNQLINQQNAQLKDKVSSLQAILSTLLVTTGNVGIPHHIPSGFPANLSTRLVTNLQFPEHSDFE*