AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045326


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045326 MtrunA17_Chr4g0061921 99.415 342 2 0 1 342 1 342 0.0 706
MsaG045326 MtrunA17_Chr3g0087171 27.742 310 183 9 18 326 17 286 1.12e-26 110
MsaG045326 MtrunA17_Chr8g0351491 24.281 313 169 12 26 332 14 264 2.55e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045326 AT5G08160.1 83.285 347 53 2 1 342 1 347 0.0 606
MsaG045326 AT5G08160.2 78.134 343 42 2 1 342 1 311 0.0 548
MsaG045326 AT2G32850.1 29.073 313 180 11 27 337 26 298 2.17e-27 113
MsaG045326 AT2G32850.2 29.073 313 180 11 27 337 26 298 2.44e-27 113
MsaG045326 AT1G12680.1 25.581 301 165 12 32 332 111 352 3.82e-12 67.4

Find 100 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGGCTTTCATTGTTT+TGG 0.155584 8:+71358381 None:intergenic
CTGGCCATACTGATGGATTT+TGG 0.264286 8:-71357849 MsaT045326.1:CDS
GGTGGAATAGTGTTCCTTCT+TGG 0.288284 8:+71358366 None:intergenic
CTTGTTATTTCCAGTTCATT+TGG 0.299548 8:-71358425 MsaT045326.1:CDS
CAGATGTTCTTCAAATATTT+CGG 0.304963 8:-71358342 MsaT045326.1:CDS
CCGGGGCCTCATAGCAGCTT+GGG 0.324444 8:+71356114 None:intergenic
GAGCTAATCTTCTTCCTTGC+GGG 0.327554 8:+71357816 None:intergenic
TCAAGAAGTGGGAGGAGATT+GGG 0.348271 8:+71358609 None:intergenic
CATTTACAATAGCCAGTTGC+AGG 0.356717 8:+71356215 None:intergenic
CAACTGGAGTGCCTCTGATC+TGG 0.364109 8:+71357794 None:intergenic
ATCAGAGGCACTCCAGTTGC+AGG 0.377493 8:-71357790 MsaT045326.1:intron
TGGATCTTATGCTATGAAAA+AGG 0.382382 8:-71358703 MsaT045326.1:CDS
CTTGGGCAATCCCACAGCTC+AGG 0.394165 8:+71357289 None:intergenic
TGCTTACGTTTATCTCGTTA+AGG 0.398078 8:-71359808 MsaT045326.1:CDS
GGATAATGATATCGTCTATC+CGG 0.398152 8:+71356095 None:intergenic
GAAATAACAAGTACGCTTCA+TGG 0.400033 8:+71358437 None:intergenic
ACGAACTGATGAAGAGCTTC+TGG 0.403819 8:+71356154 None:intergenic
ACGAGCAGCTGGAGTTGGTC+AGG 0.404228 8:-71358665 MsaT045326.1:CDS
TCCGGGGCCTCATAGCAGCT+TGG 0.408265 8:+71356113 None:intergenic
CACCTTCTCCGAGCTGCCTC+AGG 0.422367 8:+71359835 None:intergenic
ATCAAGAAGTGGGAGGAGAT+TGG 0.424760 8:+71358608 None:intergenic
TGAACGGAGGTGGTGATGTT+TGG 0.427535 8:-71359881 MsaT045326.1:CDS
TTGACATCATTGTGAGCATA+AGG 0.430332 8:+71357915 None:intergenic
ATCATATAATGCATTTAAAC+CGG 0.431617 8:+71359908 None:intergenic
TCATGCAATCATATCTGTTA+AGG 0.433133 8:-71358586 MsaT045326.1:intron
CCACCGGTAGCAGAGTCATT+AGG 0.436423 8:+71359780 None:intergenic
CTCCGAGCTGCCTCAGGATG+CGG 0.439204 8:+71359841 None:intergenic
CGTCTATATCAGCATGGCTT+GGG 0.443772 8:+71357272 None:intergenic
AATCCCACAGCTCAGGGGCT+CGG 0.444654 8:+71357296 None:intergenic
AAGAATGAATCACCTGCAAC+TGG 0.444808 8:+71357778 None:intergenic
GTCTGGTGGAAGCCTGCAAC+TGG 0.446847 8:-71356227 MsaT045326.1:CDS
TTGTAAATGCTCAGGTCAAA+TGG 0.451429 8:-71356201 MsaT045326.1:CDS
TTGGGCAATCCCACAGCTCA+GGG 0.451475 8:+71357290 None:intergenic
TTATTTCCAGTTCATTTGGA+TGG 0.458073 8:-71358421 MsaT045326.1:CDS
CAGAAAAGGACAACCACCAC+TGG 0.459960 8:-71357868 MsaT045326.1:CDS
GAATATGCACTTGGAGAGTC+TGG 0.460612 8:-71356244 MsaT045326.1:CDS
AGGACTGACGTTTGGTCACT+AGG 0.464707 8:-71357247 MsaT045326.1:CDS
CTTTCCGAGCCCCTGAGCTG+TGG 0.468523 8:-71357300 MsaT045326.1:CDS
AATGTTCCATCCAAATGAAC+TGG 0.469154 8:+71358415 None:intergenic
CATTCAGAACAACGAGCAGC+TGG 0.475734 8:-71358676 MsaT045326.1:CDS
ACTGCCAAAATCCATCAGTA+TGG 0.479140 8:+71357845 None:intergenic
CAAGAAGTGGGAGGAGATTG+GGG 0.480551 8:+71358610 None:intergenic
CCCAAGCTGCTATGAGGCCC+CGG 0.483526 8:-71356114 MsaT045326.1:CDS
GCTCAGGTCAAATGGCCAGC+TGG 0.486173 8:-71356193 MsaT045326.1:CDS
TCGTCTATATCAGCATGGCT+TGG 0.489078 8:+71357271 None:intergenic
AAAATGGGTTGCTCATTCTC+CGG 0.492235 8:-71359927 MsaT045326.1:CDS
TTTCCGAGCCCCTGAGCTGT+GGG 0.494385 8:-71357299 MsaT045326.1:CDS
TGAAGAGCTTCTGGATATGA+AGG 0.497130 8:+71356163 None:intergenic
GAACAACGAGCAGCTGGAGT+TGG 0.499688 8:-71358670 MsaT045326.1:CDS
TAGACGAGAGGACTGACGTT+TGG 0.506638 8:-71357255 MsaT045326.1:CDS
TTCAGCCCCAAGCTGCTATG+AGG 0.508302 8:-71356120 MsaT045326.1:CDS
TGTTCTTCAAATATTTCGGC+AGG 0.511807 8:-71358338 MsaT045326.1:intron
GTTCTCATAACACACAGAAA+AGG 0.516035 8:-71357882 MsaT045326.1:CDS
ACCACCACTGGCCATACTGA+TGG 0.518489 8:-71357856 MsaT045326.1:CDS
ACTGGCTATTGTAAATGCTC+AGG 0.518915 8:-71356209 MsaT045326.1:CDS
CTCTTCATCAGTTCGTGTCA+TGG 0.519184 8:-71356147 MsaT045326.1:CDS
GATAATGATATCGTCTATCC+GGG 0.519411 8:+71356096 None:intergenic
AGCCTACTCAAGAAACATCT+TGG 0.522960 8:-71358462 MsaT045326.1:CDS
ATCCTGAGGCAGCTCGGAGA+AGG 0.524053 8:-71359837 MsaT045326.1:CDS
TTCCGCATCCTGAGGCAGCT+CGG 0.538095 8:-71359843 MsaT045326.1:CDS
GGAGCTAATCTTCTTCCTTG+CGG 0.539998 8:+71357815 None:intergenic
CAAAACAATGAAAGCCAAGA+AGG 0.541448 8:-71358380 MsaT045326.1:CDS
CACAGCTCAGGGGCTCGGAA+AGG 0.548026 8:+71357301 None:intergenic
TCTCCAAGTGCATATTCAAA+CGG 0.554376 8:+71356250 None:intergenic
TATGAAGGTTTAAGTCCAGC+TGG 0.554831 8:+71356178 None:intergenic
GCATTATATGATTCTGTGAA+CGG 0.557472 8:-71359897 MsaT045326.1:CDS
CTGAGGCAGCTCGGAGAAGG+TGG 0.559462 8:-71359834 MsaT045326.1:CDS
TCAACTCAACAGCTTTGTGC+AGG 0.560174 8:-71357966 MsaT045326.1:intron
AAATAACAAGTACGCTTCAT+GGG 0.562791 8:+71358438 None:intergenic
CGGGGCCTCATAGCAGCTTG+GGG 0.569578 8:+71356115 None:intergenic
AAAGACTCTTCTCATCTCGC+AGG 0.571736 8:-71359741 MsaT045326.1:intron
TCTCCGTTTGAATATGCACT+TGG 0.573973 8:-71356253 MsaT045326.1:CDS
CGAGCAGCTGGAGTTGGTCA+GGG 0.581004 8:-71358664 MsaT045326.1:CDS
TATGATTCTGTGAACGGAGG+TGG 0.581095 8:-71359891 MsaT045326.1:CDS
TGGGCAATCCCACAGCTCAG+GGG 0.583786 8:+71357291 None:intergenic
GCTGCTCGTTGTTCTGAATG+AGG 0.586598 8:+71358679 None:intergenic
GTCCAAGATGTTTCTTGAGT+AGG 0.586917 8:+71358460 None:intergenic
GCTCCTAATGACTCTGCTAC+CGG 0.588613 8:-71359783 MsaT045326.1:CDS
GAGCTTATTAGCGAGACCAC+CGG 0.590187 8:+71359764 None:intergenic
ATGATTGCATGATCAAGAAG+TGG 0.592500 8:+71358597 None:intergenic
TGTGTTATGAGAACATTACC+AGG 0.596220 8:+71357891 None:intergenic
GCTCACAATGATGTCAAACC+TGG 0.597206 8:-71357909 MsaT045326.1:CDS
TTGGCAGTGCTCGTCCCGCA+AGG 0.600540 8:-71357830 MsaT045326.1:CDS
TCCTCTCGTCTATATCAGCA+TGG 0.609546 8:+71357266 None:intergenic
TCCATCAGTATGGCCAGTGG+TGG 0.622023 8:+71357855 None:intergenic
TGATTGCATGATCAAGAAGT+GGG 0.623676 8:+71358598 None:intergenic
GAAGATTAGCTCCAGATCAG+AGG 0.625557 8:-71357805 MsaT045326.1:CDS
ACATCATTGTGAGCATAAGG+AGG 0.631567 8:+71357918 None:intergenic
TTGCAATCAACTTATCAACA+TGG 0.641794 8:+71356074 None:intergenic
TATTTGAAGAACATCTGAGG+TGG 0.650365 8:+71358348 None:intergenic
GCCATGCTGATATAGACGAG+AGG 0.653519 8:-71357267 MsaT045326.1:CDS
AGAATCGATTCCGCATCCTG+AGG 0.654930 8:-71359851 MsaT045326.1:CDS
CCTAATGACTCTGCTACCGG+TGG 0.657390 8:-71359780 MsaT045326.1:CDS
AAATATTTGAAGAACATCTG+AGG 0.664166 8:+71358345 None:intergenic
GCAGCTGGAGTTGGTCAGGG+AGG 0.666966 8:-71358661 MsaT045326.1:CDS
TATGCACTTGGAGAGTCTGG+TGG 0.682888 8:-71356241 MsaT045326.1:CDS
TTGCATGATCAAGAAGTGGG+AGG 0.694089 8:+71358601 None:intergenic
AAATCCATCAGTATGGCCAG+TGG 0.697664 8:+71357852 None:intergenic
ATAATGATATCGTCTATCCG+GGG 0.742304 8:+71356097 None:intergenic
TTATATGATTCTGTGAACGG+AGG 0.782200 8:-71359894 MsaT045326.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 71356060 71359946 71356060 ID=MsaG045326
Chr8 mRNA 71356060 71359946 71356060 ID=MsaT045326.1;Parent=MsaG045326
Chr8 exon 71356060 71356285 71356060 ID=MsaT045326.1.exon6;Parent=MsaT045326.1
Chr8 CDS 71356060 71356285 71356060 ID=cds.MsaT045326.1;Parent=MsaT045326.1
Chr8 exon 71357226 71357350 71357226 ID=MsaT045326.1.exon5;Parent=MsaT045326.1
Chr8 CDS 71357226 71357350 71357226 ID=cds.MsaT045326.1;Parent=MsaT045326.1
Chr8 exon 71357791 71357976 71357791 ID=MsaT045326.1.exon4;Parent=MsaT045326.1
Chr8 CDS 71357791 71357976 71357791 ID=cds.MsaT045326.1;Parent=MsaT045326.1
Chr8 exon 71358339 71358482 71358339 ID=MsaT045326.1.exon3;Parent=MsaT045326.1
Chr8 CDS 71358339 71358482 71358339 ID=cds.MsaT045326.1;Parent=MsaT045326.1
Chr8 exon 71358587 71358729 71358587 ID=MsaT045326.1.exon2;Parent=MsaT045326.1
Chr8 CDS 71358587 71358729 71358587 ID=cds.MsaT045326.1;Parent=MsaT045326.1
Chr8 exon 71359742 71359946 71359742 ID=MsaT045326.1.exon1;Parent=MsaT045326.1
Chr8 CDS 71359742 71359946 71359742 ID=cds.MsaT045326.1;Parent=MsaT045326.1
Gene Sequence

>MsaT045326.1

ATGGGTTGCTCATTCTCCGGTTTAAATGCATTATATGATTCTGTGAACGGAGGTGGTGATGTTTGGATCAACGAGAATCGATTCCGCATCCTGAGGCAGCTCGGAGAAGGTGGCTTTGCTTACGTTTATCTCGTTAAGGAAGCTCCTAATGACTCTGCTACCGGTGGTCTCGCTAATAAGCTCAAAGACTCTTCTCATCTCGCAGATGATGGATCTTATGCTATGAAAAAGGTCCTCATTCAGAACAACGAGCAGCTGGAGTTGGTCAGGGAGGAAGATCGCGTCTCTTCACTGTTTAGTCACCCCAATCTCCTCCCACTTCTTGATCATGCAATCATATCTGTTAAGCCTACTCAAGAAACATCTTGGACCCATGAAGCGTACTTGTTATTTCCAGTTCATTTGGATGGAACATTGCTAGACAATGCCAAAACAATGAAAGCCAAGAAGGAACACTATTCCACCTCAGATGTTCTTCAAATATTTCGGCAGCTTTGTGCAGGACTAAAGCACATGCACAATTTAGATCCTCCTTATGCTCACAATGATGTCAAACCTGGTAATGTTCTCATAACACACAGAAAAGGACAACCACCACTGGCCATACTGATGGATTTTGGCAGTGCTCGTCCCGCAAGGAAGAAGATTAGCTCCAGATCAGAGGCACTCCAGTTGCAGGAATGGGCATCTGAACATGTCTCTGCTCCTTTCCGAGCCCCTGAGCTGTGGGATTGCCCAAGCCATGCTGATATAGACGAGAGGACTGACGTTTGGTCACTAGGATGCACATTATATGCAATAATGTATGGGGTATCTCCGTTTGAATATGCACTTGGAGAGTCTGGTGGAAGCCTGCAACTGGCTATTGTAAATGCTCAGGTCAAATGGCCAGCTGGACTTAAACCTTCATATCCAGAAGCTCTTCATCAGTTCGTGTCATGGATGCTTCAGCCCCAAGCTGCTATGAGGCCCCGGATAGACGATATCATTATCCATGTTGATAAGTTGATTGCAAAGTTCTCTCAATGA

Protein sequence

>MsaT045326.1

MGCSFSGLNALYDSVNGGGDVWINENRFRILRQLGEGGFAYVYLVKEAPNDSATGGLANKLKDSSHLADDGSYAMKKVLIQNNEQLELVREEDRVSSLFSHPNLLPLLDHAIISVKPTQETSWTHEAYLLFPVHLDGTLLDNAKTMKAKKEHYSTSDVLQIFRQLCAGLKHMHNLDPPYAHNDVKPGNVLITHRKGQPPLAILMDFGSARPARKKISSRSEALQLQEWASEHVSAPFRAPELWDCPSHADIDERTDVWSLGCTLYAIMYGVSPFEYALGESGGSLQLAIVNAQVKWPAGLKPSYPEALHQFVSWMLQPQAAMRPRIDDIIIHVDKLIAKFSQ*