AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045375


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045375 MtrunA17_Chr4g0062691 97.436 390 6 4 1 388 1 388 0.0 768
MsaG045375 MtrunA17_Chr6g0456881 41.176 170 85 3 110 266 105 272 1.39e-28 115
MsaG045375 MtrunA17_Chr7g0220931 66.129 62 21 0 133 194 122 183 3.29e-21 94.0
MsaG045375 MtrunA17_Chr1g0205131 55.696 79 33 1 119 195 95 173 1.27e-19 89.7
MsaG045375 MtrunA17_Chr8g0376571 35.329 167 83 6 127 268 57 223 3.07e-17 83.2
MsaG045375 MtrunA17_Chr8g0360441 45.918 98 51 2 109 205 31 127 4.82e-17 82.4
MsaG045375 MtrunA17_Chr7g0219461 32.035 231 129 9 132 358 58 264 1.08e-16 80.5
MsaG045375 MtrunA17_Chr2g0316391 48.718 78 39 1 113 190 3 79 3.16e-15 75.5
MsaG045375 MtrunA17_Chr6g0454781 58.621 58 24 0 132 189 50 107 4.81e-15 75.5
MsaG045375 MtrunA17_Chr4g0040921 50.000 64 32 0 127 190 18 81 7.16e-15 75.1
MsaG045375 MtrunA17_Chr3g0086961 33.099 142 87 2 132 268 58 196 1.61e-14 74.3
MsaG045375 MtrunA17_Chr2g0325171 49.206 63 32 0 127 189 20 82 1.95e-14 73.6
MsaG045375 MtrunA17_Chr4g0061561 48.276 58 30 0 132 189 58 115 4.30e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045375 AT3G18550.1 31.579 304 160 9 1 263 5 301 3.25e-25 106
MsaG045375 AT3G18550.4 31.579 304 160 9 1 263 5 301 3.25e-25 106
MsaG045375 AT3G18550.3 32.131 305 157 11 1 263 5 301 4.26e-25 106
MsaG045375 AT3G18550.2 36.538 208 109 5 1 190 5 207 7.85e-25 105
MsaG045375 AT1G67260.2 51.546 97 39 1 108 196 56 152 1.19e-23 101
MsaG045375 AT1G67260.3 51.546 97 39 1 108 196 56 152 1.19e-23 101
MsaG045375 AT1G67260.1 51.546 97 39 1 108 196 56 152 1.44e-23 100
MsaG045375 AT1G68800.2 65.217 69 23 1 122 189 102 170 2.05e-22 97.8
MsaG045375 AT1G68800.1 65.217 69 23 1 122 189 102 170 2.29e-22 97.4
MsaG045375 AT4G18390.1 35.519 183 78 6 127 293 38 196 1.05e-19 90.1
MsaG045375 AT4G18390.2 35.519 183 78 6 127 293 38 196 1.05e-19 90.1
MsaG045375 AT3G15030.2 44.898 98 53 1 94 190 7 104 3.83e-17 83.2
MsaG045375 AT3G15030.4 44.898 98 53 1 94 190 7 104 3.83e-17 83.2
MsaG045375 AT3G15030.3 44.898 98 53 1 94 190 7 104 3.83e-17 83.2
MsaG045375 AT3G15030.1 44.898 98 53 1 94 190 7 104 3.83e-17 83.2
MsaG045375 AT1G53230.1 49.383 81 40 1 112 191 29 109 5.95e-16 79.3
MsaG045375 AT1G53230.2 49.383 81 40 1 112 191 29 109 5.95e-16 79.3
MsaG045375 AT1G30210.2 36.879 141 65 3 127 267 46 162 8.12e-15 75.1
MsaG045375 AT1G30210.1 36.879 141 65 3 127 267 46 162 8.12e-15 75.1
MsaG045375 AT2G31070.1 45.000 80 43 1 111 189 8 87 5.28e-14 73.2
MsaG045375 AT3G02150.1 53.448 58 27 0 132 189 75 132 2.19e-13 70.5
MsaG045375 AT3G02150.2 53.448 58 27 0 132 189 75 132 4.09e-13 70.5
MsaG045375 AT5G60970.1 50.000 58 29 0 132 189 64 121 9.95e-13 69.3
MsaG045375 AT5G08070.2 48.276 58 30 0 132 189 34 91 1.72e-11 64.3
MsaG045375 AT5G08070.1 48.276 58 30 0 132 189 34 91 1.72e-11 64.3

Find 64 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATATCATCAGGATAATT+AGG 0.171473 8:+71940843 None:intergenic
GAGAAGAAATAGTACTAATA+AGG 0.277919 8:-71940298 MsaT045375.1:CDS
GAGAAAGGAGGAAGAGGAAA+GGG 0.310050 8:+71940879 None:intergenic
TTGCTTTCAAAGGAACAAAA+AGG 0.336731 8:+71940939 None:intergenic
CATGTTGTTGGAGGAAGATT+TGG 0.344830 8:+71940802 None:intergenic
TTTGGTCTTCAAGATATGTT+AGG 0.353164 8:-71940519 MsaT045375.1:CDS
AGAAAGGAGGAAGAGGAAAG+GGG 0.382698 8:+71940880 None:intergenic
AAGGTGTTTGATCTCAAGTT+TGG 0.394081 8:+71940452 None:intergenic
TGCTAGGTTATTTCCATTGA+GGG 0.401156 8:+71940977 None:intergenic
CAATATCATCAGGATAATTA+GGG 0.427407 8:+71940844 None:intergenic
AGAGAAAGGAGGAAGAGGAA+AGG 0.429249 8:+71940878 None:intergenic
AATTTGAACTTACTTGTTGA+AGG 0.433582 8:+71939905 None:intergenic
GCTTTGATTGTTGAGTTTGA+AGG 0.434606 8:+71940708 None:intergenic
CTTCCTGCAGCTGCTGGTAA+AGG 0.436015 8:-71940672 MsaT045375.1:CDS
TCAAGCTTGGATAATGAAGT+TGG 0.437838 8:-71940360 MsaT045375.1:CDS
TTGTTATTCATCATCACAAC+AGG 0.440557 8:+71940753 None:intergenic
TCAAAACTGCGAAAGGACTA+AGG 0.450167 8:-71940584 MsaT045375.1:CDS
CTTTGTTCATTATGAGCAAA+TGG 0.450640 8:-71939960 MsaT045375.1:CDS
TTGAGAGAATTGAACATCAT+AGG 0.458304 8:+71939931 None:intergenic
GGAAATCAACATCATGTTGT+TGG 0.463207 8:+71940790 None:intergenic
CAAGAGAAAGGGCAAGAGAA+AGG 0.468363 8:-71940233 MsaT045375.1:CDS
AGATTCTTCCACACTTTCAA+AGG 0.468610 8:+71940113 None:intergenic
TTTCAAAGGGGTTCCAAATG+AGG 0.468951 8:+71940127 None:intergenic
GATTTGGTGGCTGTCTTGAA+AGG 0.472274 8:+71940818 None:intergenic
GAAAGGAGGAAGAGGAAAGG+GGG 0.475237 8:+71940881 None:intergenic
GGGAATTGAAAGAAAGAGAA+AGG 0.476940 8:+71940864 None:intergenic
AAGAGTGCATTCAATTCTAT+AGG 0.477613 8:-71940267 MsaT045375.1:CDS
AATCTTCTTCCTGCAGCTGC+TGG 0.488028 8:-71940678 MsaT045375.1:CDS
GATTCTTCCACACTTTCAAA+GGG 0.488241 8:+71940114 None:intergenic
TCAACAAACAGTTCCCTCAA+TGG 0.498770 8:-71940990 MsaT045375.1:CDS
AAGAAAGAGAAAGGAGGAAG+AGG 0.499947 8:+71940873 None:intergenic
CAACAGGTGGATGATGAAGT+TGG 0.500835 8:+71940769 None:intergenic
GTTTGATCTCAAGTTTGGAT+TGG 0.508056 8:+71940457 None:intergenic
TGAAAGGGATCAATATCATC+AGG 0.511469 8:+71940834 None:intergenic
GTTGTTGGAGGAAGATTTGG+TGG 0.511656 8:+71940805 None:intergenic
TGAACAATTTCACCAATCCA+TGG 0.514462 8:-71939793 MsaT045375.1:CDS
TCAAAGCAATACAATGAAGA+AGG 0.515193 8:-71940162 MsaT045375.1:CDS
ATGCTAGGTTATTTCCATTG+AGG 0.517513 8:+71940976 None:intergenic
TACTGAAGGAGTTTCAAGCT+TGG 0.530351 8:-71940373 MsaT045375.1:CDS
TGAAGTTGAGTTGCTTTCAA+AGG 0.532484 8:+71940929 None:intergenic
TTGCCTTTACCAGCAGCTGC+AGG 0.547785 8:+71940669 None:intergenic
GAAAGTGTGGAAGAATCTGC+AGG 0.550929 8:-71940108 MsaT045375.1:CDS
TTGGAACCCCTTTGAAAGTG+TGG 0.551418 8:-71940121 MsaT045375.1:CDS
ATTTGGTGGCTGTCTTGAAA+GGG 0.555885 8:+71940819 None:intergenic
GTGATTTAGAGGAAAATGCT+AGG 0.566051 8:+71940961 None:intergenic
CAAAACTGCGAAAGGACTAA+GGG 0.567037 8:-71940583 MsaT045375.1:CDS
AATCAACATCATGTTGTTGG+AGG 0.575800 8:+71940793 None:intergenic
TCAACTTCTGAATGTACTGA+AGG 0.578330 8:-71940387 MsaT045375.1:CDS
CTGCGAAAGGACTAAGGGAT+CGG 0.582838 8:-71940578 MsaT045375.1:CDS
AATCAGCTTCACATGATCAA+AGG 0.583940 8:+71940050 None:intergenic
AGCAAGATCAAAACTGCGAA+AGG 0.587894 8:-71940591 MsaT045375.1:CDS
AAGCAAGCAAAACAGTGGAC+TGG 0.594986 8:-71940488 MsaT045375.1:CDS
GGTACACATCAGAGTACTCA+AGG 0.596783 8:-71940087 MsaT045375.1:CDS
TTATTCATCATCACAACAGG+TGG 0.603901 8:+71940756 None:intergenic
TGTCACTTGAAGTTGCAAAG+AGG 0.614161 8:-71940545 MsaT045375.1:CDS
AAGGGCAAGAGAAAGGACAA+AGG 0.615637 8:-71940226 MsaT045375.1:CDS
AATTGAAAGAAAGAGAAAGG+AGG 0.616668 8:+71940867 None:intergenic
AGCTTCACATGATCAAAGGA+AGG 0.617475 8:+71940054 None:intergenic
TGGTAAAGGCAAAACTGTAG+TGG 0.630471 8:-71940658 MsaT045375.1:CDS
GGTAGCATGTTAATTAGATG+CGG 0.636554 8:+71939745 None:intergenic
AGAGAATTGAACATCATAGG+TGG 0.646080 8:+71939934 None:intergenic
ATTCTTCCACACTTTCAAAG+GGG 0.682878 8:+71940115 None:intergenic
TCATGTGAAGCTGATTGATG+AGG 0.717288 8:-71940043 MsaT045375.1:CDS
ACAGTACTGATCATCATCAG+CGG 0.729484 8:-71940731 MsaT045375.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 71939758 71941018 71939758 ID=MsaG045375
Chr8 mRNA 71939758 71941018 71939758 ID=MsaT045375.1;Parent=MsaG045375
Chr8 exon 71939758 71939823 71939758 ID=MsaT045375.1.exon2;Parent=MsaT045375.1
Chr8 CDS 71939758 71939823 71939758 ID=cds.MsaT045375.1;Parent=MsaT045375.1
Chr8 exon 71939918 71941018 71939918 ID=MsaT045375.1.exon1;Parent=MsaT045375.1
Chr8 CDS 71939918 71941018 71939918 ID=cds.MsaT045375.1;Parent=MsaT045375.1
Gene Sequence

>MsaT045375.1

ATGTATTCAACAAACAGTTCCCTCAATGGAAATAACCTAGCATTTTCCTCTAAATCACCTTTTTGTTCCTTTGAAAGCAACTCAACTTCATCAACAGATATTGAAAACAGTCATTCCCCCTTTCCTCTTCCTCCTTTCTCTTTCTTTCAATTCCCTAATTATCCTGATGATATTGATCCCTTTCAAGACAGCCACCAAATCTTCCTCCAACAACATGATGTTGATTTCCAACTTCATCATCCACCTGTTGTGATGATGAATAACAACAGTACTGATCATCATCAGCGGCCTTCAAACTCAACAATCAAAGCTAACAACAATCTTCTTCCTGCAGCTGCTGGTAAAGGCAAAACTGTAGTGGATCAGCAGATCCAAAGAAAAAGATCTAGCAAGAGAGACCGACACAGCAAGATCAAAACTGCGAAAGGACTAAGGGATCGGAGAATGAGATTGTCACTTGAAGTTGCAAAGAGGTTTTTTGGTCTTCAAGATATGTTAGGCTTTGAAAAAGCAAGCAAAACAGTGGACTGGTTGCTCAACCAATCCAAACTTGAGATCAAACACCTTGCAAGAGAGAAAAATGTTAATTTTCCTACTAAAAGTGCATCTTCAACTTCTGAATGTACTGAAGGAGTTTCAAGCTTGGATAATGAAGTTGGAAATCAAGAAGAACCACAACAACAAAAACTTGTTATGAAGAGAAGAAATAGTACTAATAAGGTTTGTAGAAAGAGTGCATTCAATTCTATAGGTAGAGAAAAAGCAAGAGAAAGGGCAAGAGAAAGGACAAAGGAAAAAAAGAAGGCTAGAACATCACTAGTTGATGAATCAAATTCAAAGCAATACAATGAAGAAGGAATAAAAACTAACCTCATTTGGAACCCCTTTGAAAGTGTGGAAGAATCTGCAGGTACACATCAGAGTACTCAAGGTGTGAATCATCCTTCCTTTGATCATGTGAAGCTGATTGATGAGGCTGAAGTAGAAACAAGCTACTCTCAAAAAGCTAAGGAACAACATTCAGATGAAGATAATTCTTTGTTCATTATGAGCAAATGGAGTCCACCTATGATGTTCAATTCTCTCAATAACCTTCAACAACATCAATTTGAACAATTTCACCAATCCATGGAAAAAAATTGGGAGGCATACAACAACCGCATCTAA

Protein sequence

>MsaT045375.1

MYSTNSSLNGNNLAFSSKSPFCSFESNSTSSTDIENSHSPFPLPPFSFFQFPNYPDDIDPFQDSHQIFLQQHDVDFQLHHPPVVMMNNNSTDHHQRPSNSTIKANNNLLPAAAGKGKTVVDQQIQRKRSSKRDRHSKIKTAKGLRDRRMRLSLEVAKRFFGLQDMLGFEKASKTVDWLLNQSKLEIKHLAREKNVNFPTKSASSTSECTEGVSSLDNEVGNQEEPQQQKLVMKRRNSTNKVCRKSAFNSIGREKARERARERTKEKKKARTSLVDESNSKQYNEEGIKTNLIWNPFESVEESAGTHQSTQGVNHPSFDHVKLIDEAEVETSYSQKAKEQHSDEDNSLFIMSKWSPPMMFNSLNNLQQHQFEQFHQSMEKNWEAYNNRI*