AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG045396

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045396 MtrunA17_Chr4g0062881 93.217 457 31 0 307 763 1 457 0.0 874
MsaG045396 MtrunA17_Chr1g0171711 38.433 804 405 22 22 763 11 786 3.58e-146 447
MsaG045396 MtrunA17_Chr1g0148791 50.472 424 174 9 375 763 480 902 1.48e-120 384
MsaG045396 MtrunA17_Chr1g0148791 37.344 241 134 7 142 375 182 412 5.29e-29 124
MsaG045396 MtrunA17_Chr1g0148791 44.872 78 42 1 22 98 11 88 2.37e-15 80.5
MsaG045396 MtrunA17_Chr4g0062891 89.474 190 20 0 23 212 22 211 8.07e-119 356
MsaG045396 MtrunA17_Chr3g0138311 49.061 426 175 10 375 763 477 897 4.25e-118 377
MsaG045396 MtrunA17_Chr3g0138311 36.100 241 140 7 142 375 171 404 7.98e-29 123
MsaG045396 MtrunA17_Chr7g0237271 33.583 801 440 25 22 763 17 784 3.14e-117 372
MsaG045396 MtrunA17_Chr7g0237241 33.543 796 438 26 22 763 17 775 1.90e-115 367
MsaG045396 MtrunA17_Chr2g0282401 41.275 298 164 6 471 763 497 788 4.51e-65 231
MsaG045396 MtrunA17_Chr4g0047051 35.889 287 177 6 481 763 25 308 4.56e-47 169
MsaG045396 MtrunA17_Chr4g0047091 35.417 288 175 8 482 763 84 366 1.03e-40 153
MsaG045396 MtrunA17_Chr4g0047071 33.333 285 172 8 492 763 3 282 1.11e-36 139
MsaG045396 MtrunA17_Chr4g0047061 35.887 248 146 8 522 763 90 330 2.15e-35 137
MsaG045396 MtrunA17_Chr4g0047101 37.118 229 120 8 492 712 32 244 3.15e-31 123
MsaG045396 MtrunA17_Chr3g0093681 28.369 282 184 9 492 762 278 552 6.58e-22 100
MsaG045396 MtrunA17_Chr5g0405851 29.470 302 178 12 488 762 156 449 7.04e-22 99.8
MsaG045396 MtrunA17_Chr1g0172501 28.369 282 184 9 492 762 278 552 1.71e-21 99.4
MsaG045396 MtrunA17_Chr6g0448921 26.613 248 172 6 483 724 13 256 4.63e-18 89.0
MsaG045396 MtrunA17_Chr4g0047111 32.886 149 71 5 567 712 5 127 4.54e-17 79.0
MsaG045396 MtrunA17_Chr3g0136971 28.899 218 141 7 486 697 419 628 9.00e-17 84.7
MsaG045396 MtrunA17_Chr1g0185501 23.427 286 204 10 489 762 30 312 1.62e-14 75.5
MsaG045396 MtrunA17_Chr2g0281451 23.715 253 183 6 478 724 7 255 3.28e-13 73.6
MsaG045396 MtrunA17_Chr2g0281451 25.131 191 137 4 492 678 106 294 1.67e-11 68.2
MsaG045396 MtrunA17_Chr4g0067151 25.764 229 152 6 516 729 15 240 6.02e-13 70.9
MsaG045396 MtrunA17_Chr4g0067151 27.136 199 131 6 494 681 86 281 9.66e-13 70.1
MsaG045396 MtrunA17_Chr4g0067151 25.980 204 139 6 486 678 120 322 8.78e-12 67.4
MsaG045396 MtrunA17_Chr8g0392701 26.829 246 158 11 500 730 30 268 4.13e-12 68.2
MsaG045396 MtrunA17_Chr2g0308391 22.443 352 230 12 445 763 91 432 5.19e-12 68.9
MsaG045396 MtrunA17_Chr4g0035611 22.183 284 181 10 489 737 54 332 2.81e-11 67.4
MsaG045396 MtrunA17_Chr1g0168391 24.865 185 131 5 500 677 238 421 2.83e-11 66.6
MsaG045396 MtrunA17_Chr2g0295791 23.551 276 195 8 501 763 185 457 7.79e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG045396 AT2G32700.6 36.567 804 419 25 22 763 11 785 1.68e-132 412
MsaG045396 AT2G32700.2 36.476 806 419 26 22 763 11 787 1.14e-130 407
MsaG045396 AT2G32700.5 36.476 806 419 26 22 763 11 787 1.14e-130 407
MsaG045396 AT2G32700.1 36.476 806 419 26 22 763 11 787 1.14e-130 407
MsaG045396 AT2G32700.4 36.476 806 419 26 22 763 11 787 1.14e-130 407
MsaG045396 AT2G32700.3 36.476 806 419 26 22 763 11 787 1.14e-130 407
MsaG045396 AT2G32700.7 36.186 818 424 26 22 763 11 806 3.10e-130 407
MsaG045396 AT4G32551.1 49.517 414 180 7 378 763 519 931 1.26e-122 390
MsaG045396 AT4G32551.1 36.111 252 147 8 126 371 195 438 4.78e-29 124
MsaG045396 AT4G32551.1 46.154 78 41 1 22 98 11 88 6.35e-16 82.8
MsaG045396 AT4G32551.2 49.517 414 180 7 378 763 557 969 5.90e-122 389
MsaG045396 AT4G32551.2 31.724 290 146 9 126 371 195 476 6.96e-24 108
MsaG045396 AT4G32551.2 46.154 78 41 1 22 98 11 88 7.20e-16 82.8
MsaG045396 AT2G41500.1 29.787 282 180 9 492 762 261 535 2.75e-22 102
MsaG045396 AT2G41500.2 29.787 282 180 9 492 762 261 535 2.75e-22 102
MsaG045396 AT1G61210.1 25.869 259 179 7 472 724 4 255 4.84e-18 89.7
MsaG045396 AT1G61210.3 25.869 259 179 7 472 724 4 255 5.00e-18 89.7
MsaG045396 AT1G61210.2 25.869 259 179 7 472 724 4 255 6.20e-18 89.4
MsaG045396 AT5G23430.3 27.490 251 166 7 483 724 13 256 2.22e-17 87.4
MsaG045396 AT5G23430.2 27.490 251 166 7 483 724 13 256 2.22e-17 87.4
MsaG045396 AT5G23430.1 27.490 251 166 7 483 724 13 256 2.26e-17 87.4
MsaG045396 AT5G25150.2 29.864 221 141 7 483 697 415 627 2.26e-17 87.0
MsaG045396 AT5G25150.1 29.864 221 141 7 483 697 415 627 2.28e-17 87.0
MsaG045396 AT5G08390.1 26.693 251 168 7 483 724 13 256 1.31e-16 85.1
MsaG045396 AT3G49660.1 24.658 292 196 12 489 762 27 312 1.23e-15 79.3
MsaG045396 AT3G49660.1 24.125 257 175 9 523 763 19 271 2.08e-13 72.4
MsaG045396 AT1G11160.1 23.552 259 185 7 472 724 4 255 4.74e-15 80.1
MsaG045396 AT1G11160.1 25.521 192 137 4 491 678 105 294 5.50e-12 70.1
MsaG045396 AT1G11160.2 23.552 259 185 7 472 724 4 255 6.45e-15 79.7
MsaG045396 AT1G11160.2 25.521 192 137 4 491 678 105 294 6.58e-12 69.7
MsaG045396 AT1G11160.4 23.552 259 185 7 472 724 4 255 7.08e-15 79.3
MsaG045396 AT1G11160.4 25.521 192 137 4 491 678 105 294 6.31e-12 70.1
MsaG045396 AT5G60940.1 24.262 305 197 11 489 763 126 426 2.68e-14 76.3
MsaG045396 AT4G02730.1 23.529 289 203 9 489 763 46 330 4.43e-14 74.7
MsaG045396 AT5G60940.2 24.262 305 197 11 489 763 34 334 6.28e-14 74.3
MsaG045396 AT5G13480.1 23.297 279 199 9 494 762 131 404 1.67e-13 74.7
MsaG045396 AT5G13480.4 23.297 279 199 9 494 762 119 392 1.90e-13 74.3
MsaG045396 AT5G13480.3 23.297 279 199 9 494 762 119 392 2.20e-13 74.3
MsaG045396 AT5G13480.2 23.297 279 199 9 494 762 142 415 2.79e-13 73.9
MsaG045396 AT2G33340.2 25.668 187 129 4 500 677 236 421 1.40e-12 71.2
MsaG045396 AT2G33340.1 25.668 187 129 4 500 677 236 421 1.40e-12 71.2
MsaG045396 AT2G33340.3 25.668 187 129 4 500 677 236 421 1.50e-12 71.2
MsaG045396 AT1G11160.3 25.789 190 135 4 493 678 65 252 5.55e-12 70.1
MsaG045396 AT5G08560.1 23.904 251 175 8 524 762 270 516 1.33e-11 68.6
MsaG045396 AT5G08560.1 25.000 292 198 13 486 762 274 559 7.41e-11 65.9
MsaG045396 AT5G08560.2 23.904 251 175 8 524 762 270 516 1.33e-11 68.6
MsaG045396 AT5G08560.2 25.000 292 198 13 486 762 274 559 7.41e-11 65.9
MsaG045396 AT5G08560.3 23.904 251 175 8 524 762 270 516 1.33e-11 68.6
MsaG045396 AT5G08560.3 25.000 292 198 13 486 762 274 559 7.41e-11 65.9
MsaG045396 AT5G67320.1 27.778 162 104 4 488 637 450 610 5.14e-11 66.6
MsaG045396 AT2G43770.1 20.280 286 204 11 494 761 61 340 7.87e-11 64.7

Find 181 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTCTTCGGACATTTAC+AGG 0.170744 8:-72325432 MsaT045396.1:CDS
GCTCTTCAAGCGTAGATTTC+AGG 0.213054 8:+72326301 None:intergenic
CACCATAGGAGCATTGTTTA+TGG 0.233609 8:+72330303 None:intergenic
CTTCCGGTCAACTTGACTTT+TGG 0.234717 8:+72327372 None:intergenic
CCTGCTCAGCTGCAGCAATA+TGG 0.235792 8:-72330367 MsaT045396.1:CDS
AGTACCATGAACACTGTAAA+TGG 0.257174 8:+72329631 None:intergenic
TATTTGTTCTTGTGATAATA+AGG 0.266795 8:-72325361 MsaT045396.1:CDS
AAGTCCAGATGAGGCAATTC+AGG 0.270725 8:+72327552 None:intergenic
TCAGTGTGCTGGCACTCTTC+TGG 0.277434 8:-72324755 MsaT045396.1:CDS
GCGATTTCATACGCTTTATC+AGG 0.280157 8:+72332049 None:intergenic
GAACAAATGTAATACCTTAA+AGG 0.280380 8:+72330186 None:intergenic
GCTTGTCCATAATCAAAATA+TGG 0.283609 8:-72327652 MsaT045396.1:CDS
TTGTGCAGGTGTTACAATAT+TGG 0.283850 8:+72327584 None:intergenic
TGTGCAGGTGTTACAATATT+GGG 0.283894 8:+72327585 None:intergenic
CTGCTCAGCTGCAGCAATAT+GGG 0.293474 8:-72330366 MsaT045396.1:CDS
CCATATTGCTGCAGCTGAGC+AGG 0.294656 8:+72330367 None:intergenic
GATGGAAAACTATTTGTTAC+TGG 0.314915 8:-72326496 MsaT045396.1:CDS
TACTGGTGGTCGTGATAAAA+AGG 0.316571 8:-72326479 MsaT045396.1:intron
TTGCAAATGGTAGTTGTATT+GGG 0.320162 8:-72325320 MsaT045396.1:CDS
TTTCCCTGAATTGCCTCATC+TGG 0.320474 8:-72327556 MsaT045396.1:CDS
CAGGTTGAAGACTCCCCTTC+AGG 0.322856 8:-72330230 MsaT045396.1:CDS
ATAACTGGTGGACAGCCTTC+AGG 0.329589 8:-72330007 MsaT045396.1:CDS
TGTTATTTGGCAGGCTCATA+TGG 0.330746 8:-72326830 MsaT045396.1:intron
ATTGCAAATGGTAGTTGTAT+TGG 0.340157 8:-72325321 MsaT045396.1:CDS
CTCCTATGGTGAGGCAGAAC+CGG 0.346361 8:-72330291 MsaT045396.1:CDS
ATTGTAACACCTGCACAAAT+TGG 0.348653 8:-72327579 MsaT045396.1:CDS
TAATTCAGTCGCTTATGCTC+TGG 0.350259 8:-72324480 MsaT045396.1:intron
GTGAGATTTCAACCTGGTTT+GGG 0.350453 8:-72325181 MsaT045396.1:CDS
CAAATAAGGCCACGTTTACT+TGG 0.352863 8:+72325378 None:intergenic
TCTCAAGAGCAACGGATCTT+TGG 0.353331 8:-72326803 MsaT045396.1:CDS
TATTATCACAAGAACAAATA+AGG 0.354896 8:+72325364 None:intergenic
GCACTCTTCTGGAAATTATC+TGG 0.356961 8:-72324744 MsaT045396.1:CDS
CCGATAAAACTGTAAAGGTT+TGG 0.363852 8:-72326215 MsaT045396.1:CDS
GGTGAGATTTCAACCTGGTT+TGG 0.364221 8:-72325182 MsaT045396.1:CDS
GAAGGCTGTCCACCAGTTAT+TGG 0.366035 8:+72330010 None:intergenic
TTTATCAGGTTCATCGCATT+TGG 0.370622 8:+72332063 None:intergenic
GGCAATGTTGAACAGAATAT+TGG 0.370914 8:-72331328 MsaT045396.1:intron
CTTGGTCAACTCGTCCATTA+TGG 0.371045 8:+72326740 None:intergenic
AATCAAAACCAGCAACTTCC+AGG 0.378139 8:-72329814 MsaT045396.1:CDS
CTTGTAACCCTGGAAGTTGC+TGG 0.378969 8:+72329806 None:intergenic
AACACAATGGGATCATCTTC+TGG 0.380898 8:-72327295 MsaT045396.1:CDS
ACCATAGGAGCATTGTTTAT+GGG 0.382702 8:+72330304 None:intergenic
ACGGCAGCAACTTATGTTTC+AGG 0.387564 8:-72327733 MsaT045396.1:CDS
CATATTGCTGCAGCTGAGCA+GGG 0.392189 8:+72330368 None:intergenic
CGATAAAACTGTAAAGGTTT+GGG 0.397292 8:-72326214 MsaT045396.1:CDS
TGCAGGCTGGTCATTCTCTT+CGG 0.397318 8:-72325443 MsaT045396.1:intron
GTTTGCAGTTCCAGAGCTAT+TGG 0.397684 8:+72327315 None:intergenic
ACCAGCATTATCTTACCTTA+AGG 0.398546 8:-72331108 MsaT045396.1:intron
AAGGTTGGTTGATACCTTAT+GGG 0.400769 8:+72327521 None:intergenic
GGATCCATTTACAGTGTTCA+TGG 0.401695 8:-72329635 MsaT045396.1:intron
ACTTGGCAACAGAATGGTTT+CGG 0.403735 8:-72327235 MsaT045396.1:CDS
CCATCAAAACCACTGACAAA+TGG 0.409375 8:+72327690 None:intergenic
CAGGGCTCTGCAATATGAAC+TGG 0.414323 8:+72330386 None:intergenic
GGCATCTGTTTGTAATGATC+TGG 0.417806 8:-72324723 MsaT045396.1:CDS
AGCCAAAATCTTACTGCTAA+AGG 0.419763 8:+72328662 None:intergenic
GAGTTGCAGATTTAAATTGC+AGG 0.422031 8:-72325101 MsaT045396.1:intron
CTGCCGTGGTGAAAATTGAT+TGG 0.433947 8:+72327749 None:intergenic
AACGAAATAGCACCAATAAC+TGG 0.436676 8:-72330022 MsaT045396.1:CDS
CTTGCTGATTCAGACCATAA+TGG 0.437173 8:-72326754 MsaT045396.1:CDS
ATGATAGGACTTTCAAGATC+TGG 0.438220 8:-72324357 MsaT045396.1:CDS
AATTCAGTCGCTTATGCTCT+GGG 0.438597 8:-72324479 MsaT045396.1:intron
TTTACAAGAGAAACTTCAAC+AGG 0.441555 8:-72329842 MsaT045396.1:CDS
ATAATAAGGAGATAAGATAT+TGG 0.444385 8:-72325347 MsaT045396.1:CDS
ATGAGATTCAGGCTTCTCTA+TGG 0.444675 8:-72326341 MsaT045396.1:intron
CTCTGGGCCATTGGATCTGA+TGG 0.445049 8:-72324695 MsaT045396.1:CDS
CCATTGGATCTGATGGCAGC+GGG 0.448766 8:-72324688 MsaT045396.1:CDS
GCTTATGCTCTGGGACTTTG+AGG 0.454130 8:-72324470 MsaT045396.1:CDS
TGCTCAGCTGCAGCAATATG+GGG 0.454725 8:-72330365 MsaT045396.1:CDS
TTGGCAGTATCAGATGTTAC+TGG 0.456091 8:-72324400 MsaT045396.1:CDS
TAATGATCTGGTTAAACTCT+GGG 0.456770 8:-72324711 MsaT045396.1:CDS
TCCCATAAACAATGCTCCTA+TGG 0.458377 8:-72330305 MsaT045396.1:CDS
GCCTTGTTCGTTCAGTGTGC+TGG 0.458741 8:-72324766 MsaT045396.1:CDS
TAACTGGTGGACAGCCTTCA+GGG 0.459341 8:-72330006 MsaT045396.1:intron
AACAAGGCACAATCCTCGTT+TGG 0.460063 8:+72330049 None:intergenic
CTGGTTAAACTCTGGGCCAT+TGG 0.460194 8:-72324704 MsaT045396.1:CDS
GTAATGATCTGGTTAAACTC+TGG 0.461731 8:-72324712 MsaT045396.1:CDS
TGCACTCATGCCACTGTGCA+TGG 0.464962 8:+72329688 None:intergenic
TGATTATGGACAAGCAAACT+TGG 0.465215 8:+72327660 None:intergenic
AACCACCTACAGAATTTGAT+TGG 0.466272 8:+72327613 None:intergenic
ATAATCAAAATATGGTTCCT+TGG 0.467781 8:-72327644 MsaT045396.1:CDS
TCAGGTCTTGCCAATAGCTC+TGG 0.469604 8:-72327325 MsaT045396.1:CDS
CGAAAAGTAGGATCAAGTTC+AGG 0.470472 8:-72327343 MsaT045396.1:CDS
TCAAAATATTGTAGCTAGGA+TGG 0.471613 8:+72324612 None:intergenic
TGCAGACACGACTGCCAACT+TGG 0.472292 8:-72327252 MsaT045396.1:CDS
GGCAGTCGTGTCTGCATAGT+TGG 0.473371 8:+72327259 None:intergenic
TTCTGTTTGCAGACAAACAT+TGG 0.483931 8:-72329881 MsaT045396.1:intron
GCAAGAATATTTCATGATGA+AGG 0.484394 8:-72331349 MsaT045396.1:CDS
ACTTATGTTTCAGGCACAGC+AGG 0.484632 8:-72327724 MsaT045396.1:CDS
AAACCATTCTGTTGCCAAGT+TGG 0.494388 8:+72327238 None:intergenic
GCCATTGGATCTGATGGCAG+CGG 0.497982 8:-72324689 MsaT045396.1:CDS
TCCTATGGTGAGGCAGAACC+GGG 0.500407 8:-72330290 MsaT045396.1:CDS
TGCATCAACACAGAATCAAT+TGG 0.506774 8:-72327206 MsaT045396.1:intron
TCTGGAACTGCAAACACAAT+GGG 0.508959 8:-72327307 MsaT045396.1:CDS
ATCAGATGTTACTGGATTGG+TGG 0.509104 8:-72324392 MsaT045396.1:CDS
TAATGAGATCAAGCATGGAT+TGG 0.510535 8:-72326557 MsaT045396.1:CDS
TGGCCTCAGTTAGTCATGAT+AGG 0.512839 8:-72324372 MsaT045396.1:CDS
GATACAAGCTTGTCGTGTGC+AGG 0.517006 8:+72324427 None:intergenic
TGTTGTCACTTCTCCTCGGA+TGG 0.520645 8:-72326514 MsaT045396.1:CDS
CTGCCAACTTGGCAACAGAA+TGG 0.522284 8:-72327241 MsaT045396.1:CDS
AGATATTGGAATATTGCAAA+TGG 0.522398 8:-72325333 MsaT045396.1:CDS
TTGTAAAATGCTAAGTGCAC+TGG 0.525997 8:+72329858 None:intergenic
CCCGCTGCCATCAGATCCAA+TGG 0.526518 8:+72324688 None:intergenic
TCATTTAATGAGATCAAGCA+TGG 0.527559 8:-72326562 MsaT045396.1:CDS
ATTTCAACCTGGTTTGGGAA+AGG 0.527606 8:-72325176 MsaT045396.1:CDS
TAGCAACATAGAACATGCTT+GGG 0.529135 8:+72326247 None:intergenic
TCAGGGAAACCAATTTGTGC+AGG 0.529698 8:+72327570 None:intergenic
CAAGAGCAACGGATCTTTGG+CGG 0.529755 8:-72326800 MsaT045396.1:CDS
GCAAGAACCTTTCCCAAACC+AGG 0.531493 8:+72325169 None:intergenic
CATACAATTGATTCTTCATG+AGG 0.532585 8:+72331377 None:intergenic
GTTGTTCTCTCTTCGAGTGG+TGG 0.533117 8:-72331181 MsaT045396.1:CDS
AGTCCAGATGAGGCAATTCA+GGG 0.534232 8:+72327553 None:intergenic
ATGATCAAAATATTGTAGCT+AGG 0.535188 8:+72324608 None:intergenic
CTCAGCCGATAAAACTGTAA+AGG 0.537151 8:-72326220 MsaT045396.1:CDS
CATCAAAAGCATTTCCCTGA+AGG 0.539630 8:+72330215 None:intergenic
GTAGCAACATAGAACATGCT+TGG 0.539825 8:+72326246 None:intergenic
TGCTATTTCGTTCAACAACA+AGG 0.543891 8:+72330033 None:intergenic
CTCGTTTGGAAGCTGACCGC+GGG 0.545244 8:+72330063 None:intergenic
CCGGGTTACTTCTAATACAA+TGG 0.545781 8:-72330272 MsaT045396.1:CDS
AATGGCAACAAAACTAAATG+AGG 0.546151 8:-72330254 MsaT045396.1:CDS
GGACTTAGATATGACCCATA+AGG 0.547552 8:-72327535 MsaT045396.1:intron
CCATTGTATTAGAAGTAACC+CGG 0.548960 8:+72330272 None:intergenic
CGACAAGCTTGTATCTGCAT+TGG 0.551855 8:-72324419 MsaT045396.1:CDS
TCTCAAGCTGTTGTTTCAGA+AGG 0.560493 8:-72329656 MsaT045396.1:CDS
TTTGATTGGCCATCGTTCCA+AGG 0.560547 8:+72327627 None:intergenic
GTCTGCATAGTTGGTGTAGA+GGG 0.561086 8:+72327268 None:intergenic
CTTCCAAAAGTCAAGTTGAC+CGG 0.561136 8:-72327375 MsaT045396.1:CDS
TGACTGTTGTCACTTCTCCT+CGG 0.561258 8:-72326518 MsaT045396.1:CDS
AGGTTGAAGACTCCCCTTCA+GGG 0.566579 8:-72330229 MsaT045396.1:CDS
GGTAGCGACTTGACTCTGAA+AGG 0.568593 8:-72328689 MsaT045396.1:CDS
GTAGCGACTTGACTCTGAAA+GGG 0.570038 8:-72328688 MsaT045396.1:CDS
ATCAAAAGCATTTCCCTGAA+GGG 0.572579 8:+72330216 None:intergenic
CTCTGGAACTGCAAACACAA+TGG 0.574440 8:-72327308 MsaT045396.1:CDS
TTATCAGGGTGGTGTAACTC+AGG 0.575396 8:-72325203 MsaT045396.1:intron
CTGGTTGTTCTCTCTTCGAG+TGG 0.576679 8:-72331184 MsaT045396.1:CDS
CAACAAAACTAAATGAGGAC+AGG 0.577019 8:-72330249 MsaT045396.1:CDS
ACCTTAAGGTAAGATAATGC+TGG 0.580228 8:+72331107 None:intergenic
CGCTTGAAGAGCACACTCAA+CGG 0.586936 8:-72326290 MsaT045396.1:CDS
TTTACGATTACTTGTAACCC+TGG 0.588248 8:+72329796 None:intergenic
GAATTGTTTATGGCCAAACA+AGG 0.589606 8:-72331146 MsaT045396.1:CDS
CTGAACGAACAAGGCTCATG+TGG 0.591408 8:+72324774 None:intergenic
TTAGTTAATCCATTTGTCAG+TGG 0.592463 8:-72327699 MsaT045396.1:CDS
AAGATCCGTTGCTCTTGAGA+TGG 0.593548 8:+72326806 None:intergenic
AGTATCAGATGTTACTGGAT+TGG 0.595920 8:-72324395 MsaT045396.1:CDS
AATATGGTTCCTTGGAACGA+TGG 0.597683 8:-72327636 MsaT045396.1:CDS
ATTCTCTTCGGACATTTACA+GGG 0.599684 8:-72325431 MsaT045396.1:CDS
ACCCGGTTCTGCCTCACCAT+AGG 0.601611 8:+72330289 None:intergenic
GCCAGCACACTGAACGAACA+AGG 0.603185 8:+72324765 None:intergenic
AGGTGTTACAATATTGGGAA+TGG 0.603227 8:+72327590 None:intergenic
ATCGCATTTGGATTGAGACA+TGG 0.604361 8:+72332075 None:intergenic
TCAAAAGCATTTCCCTGAAG+GGG 0.605318 8:+72330217 None:intergenic
GGAAAACTATTTGTTACTGG+TGG 0.605813 8:-72326493 MsaT045396.1:CDS
ATCAAAACCAGCAACTTCCA+GGG 0.612502 8:-72329813 MsaT045396.1:CDS
GTTTATGGGACAGAGAACAT+TGG 0.619165 8:+72330318 None:intergenic
GGCCAATCAAATTCTGTAGG+TGG 0.625840 8:-72327615 MsaT045396.1:CDS
CCGCGGTCAGCTTCCAAACG+AGG 0.626261 8:-72330062 MsaT045396.1:CDS
TGTCTGCATAGTTGGTGTAG+AGG 0.626946 8:+72327267 None:intergenic
GATGGCCAATCAAATTCTGT+AGG 0.638312 8:-72327618 MsaT045396.1:CDS
CATTGGATCTGATGGCAGCG+GGG 0.639120 8:-72324687 MsaT045396.1:CDS
ATGGACCATCTCAAGAGCAA+CGG 0.642745 8:-72326811 MsaT045396.1:CDS
AATTGATTGGAAGATTGTGA+AGG 0.646797 8:+72327762 None:intergenic
GCGACTTGACTCTGAAAGGG+TGG 0.648057 8:-72328685 MsaT045396.1:CDS
ATGATTAGTAGGCACCCTGA+AGG 0.649924 8:+72329992 None:intergenic
GTCATATCTAAGTCCAGATG+AGG 0.650884 8:+72327543 None:intergenic
GAAATAGCACCAATAACTGG+TGG 0.658266 8:-72330019 MsaT045396.1:CDS
GGGATTGGATGAGCATCATG+CGG 0.660736 8:-72327814 MsaT045396.1:CDS
TAAACAATGCTCCTATGGTG+AGG 0.661726 8:-72330300 MsaT045396.1:CDS
TGAAACATAAGTTGCTGCCG+TGG 0.662848 8:+72327735 None:intergenic
CCTCGTTTGGAAGCTGACCG+CGG 0.663224 8:+72330062 None:intergenic
TTTGATCATTTGCTGCAGTG+AGG 0.663983 8:-72324594 MsaT045396.1:intron
AAATTTAGGCTCAAGAAATG+CGG 0.670041 8:-72330435 MsaT045396.1:intron
TATGGGGCTGAAACTCAAGA+AGG 0.670598 8:-72330349 MsaT045396.1:CDS
ACTCAGGTGAGATTTCAACC+TGG 0.671694 8:-72325187 MsaT045396.1:CDS
AGTCCTATCATGACTAACTG+AGG 0.678070 8:+72324369 None:intergenic
CACTCGAAGAGAGAACAACC+AGG 0.686015 8:+72331185 None:intergenic
GACTTTCATCCAAGTAAACG+TGG 0.686140 8:-72325387 MsaT045396.1:CDS
GGTGTAGTTATGGATGCACC+TGG 0.690437 8:-72331203 MsaT045396.1:intron
CATAATGGACGAGTTGACCA+AGG 0.691350 8:-72326739 MsaT045396.1:intron
GTGGCATGAGTGCAATGATG+AGG 0.712221 8:-72329679 MsaT045396.1:CDS
CTGAATCAGGCCATGCACAG+TGG 0.713979 8:-72329698 MsaT045396.1:intron
ATAATGAATGCTTATACCCG+CGG 0.714622 8:-72330079 MsaT045396.1:CDS
GTAGCTAGGATGGAAAACGC+AGG 0.717458 8:+72324622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr8 gene 72324351 72332095 72324351 ID=MsaG045396
Chr8 mRNA 72324351 72332095 72324351 ID=MsaT045396.1;Parent=MsaG045396
Chr8 exon 72324351 72324494 72324351 ID=MsaT045396.1.exon17;Parent=MsaT045396.1
Chr8 CDS 72324351 72324494 72324351 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72324595 72324798 72324595 ID=MsaT045396.1.exon16;Parent=MsaT045396.1
Chr8 CDS 72324595 72324798 72324595 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72325102 72325218 72325102 ID=MsaT045396.1.exon15;Parent=MsaT045396.1
Chr8 CDS 72325102 72325218 72325102 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72325311 72325460 72325311 ID=MsaT045396.1.exon14;Parent=MsaT045396.1
Chr8 CDS 72325311 72325460 72325311 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72326203 72326352 72326203 ID=MsaT045396.1.exon13;Parent=MsaT045396.1
Chr8 CDS 72326203 72326352 72326203 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72326480 72326589 72326480 ID=MsaT045396.1.exon12;Parent=MsaT045396.1
Chr8 CDS 72326480 72326589 72326480 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72326740 72326839 72326740 ID=MsaT045396.1.exon11;Parent=MsaT045396.1
Chr8 CDS 72326740 72326839 72326740 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72327207 72327398 72327207 ID=MsaT045396.1.exon10;Parent=MsaT045396.1
Chr8 CDS 72327207 72327398 72327207 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72327536 72327835 72327536 ID=MsaT045396.1.exon9;Parent=MsaT045396.1
Chr8 CDS 72327536 72327835 72327536 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72328676 72328725 72328676 ID=MsaT045396.1.exon8;Parent=MsaT045396.1
Chr8 CDS 72328676 72328725 72328676 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72329636 72329711 72329636 ID=MsaT045396.1.exon7;Parent=MsaT045396.1
Chr8 CDS 72329636 72329711 72329636 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72329807 72329891 72329807 ID=MsaT045396.1.exon6;Parent=MsaT045396.1
Chr8 CDS 72329807 72329891 72329807 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72330007 72330101 72330007 ID=MsaT045396.1.exon5;Parent=MsaT045396.1
Chr8 CDS 72330007 72330101 72330007 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72330201 72330449 72330201 ID=MsaT045396.1.exon4;Parent=MsaT045396.1
Chr8 CDS 72330201 72330449 72330201 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72331109 72331218 72331109 ID=MsaT045396.1.exon3;Parent=MsaT045396.1
Chr8 CDS 72331109 72331218 72331109 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72331329 72331423 72331329 ID=MsaT045396.1.exon2;Parent=MsaT045396.1
Chr8 CDS 72331329 72331423 72331329 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Chr8 exon 72332031 72332095 72332031 ID=MsaT045396.1.exon1;Parent=MsaT045396.1
Chr8 CDS 72332031 72332095 72332031 ID=cds.MsaT045396.1;Parent=MsaT045396.1
Gene Sequence

>MsaT045396.1

ATGTCTCAATCCAAATGCGATGAACCTGATAAAGCGTATGAAATCGCTGAACTCATCGCCATTTTGTTAGATGCTTTTATCTACGATTACCTCATGAAGAATCAATTGTATGAAACTGCAAGAATATTTCATGATGAAGGCAATGTTGAACAGAATATTGTTATGGATGCACCTGGTTGTTCTCTCTTCGAGTGGTGGTCTGTTTTCTGGGAATTGTTTATGGCCAAACAAGGTTTTTCACTCTCAGAACCAGCATTATCTTACCTTAAGGCTCAAGAAATGCGGAAGCAAGAAGAAGAGATAGAAAAAATCCAGTTCATATTGCAGAGCCCTGCTCAGCTGCAGCAATATGGGGCTGAAACTCAAGAAGGTGTTAATGCCAATGTTCTCTGTCCCATAAACAATGCTCCTATGGTGAGGCAGAACCGGGTTACTTCTAATACAATGGCAACAAAACTAAATGAGGACAGGTTGAAGACTCCCCTTCAGGGAAATGCTTTTGATGACAGGACCTTTAAGATAATGAATGCTTATACCCGCGGTCAGCTTCCAAACGAGGATTGTGCCTTGTTGTTGAACGAAATAGCACCAATAACTGGTGGACAGCCTTCAGGACAAACATTGGCCAGTGCACTTAGCATTTTACAAGAGAAACTTCAACAGGATCAAAATCAAAACCAGCAACTTCCAGGGTTACAAGCCATGCACAGTGGCATGAGTGCAATGATGAGGTCTCAAGCTGTTGTTTCAGAAGGATCCATTTACAGTGTTCATGGTTCAAATCAAGGTGGTAGCGACTTGACTCTGAAAGGGTGGCCTTTAGCAGGATTGGATGAGCATCATGCGGAACTTCTTCATAAGCATAATTTGATTCTGCCTTCACAATCTTCCAATCAATTTTCACCACGGCAGCAACTTATGTTTCAGGCACAGCAGGATTTAGTTAATCCATTTGTCAGTGGTTTTGATGGCAAAAGACCAAGTTTGCTTGTCCATAATCAAAATATGGTTCCTTGGAACGATGGCCAATCAAATTCTGTAGGTGGTTCCATTCCCAATATTGTAACACCTGCACAAATTGGTTTCCCTGAATTGCCTCATCTGGACTTAGATATGACCCATAAGGCTTCCAAAAGTCAAGTTGACCGGAAGCAAAAACGAAAAGTAGGATCAAGTTCAGGTCTTGCCAATAGCTCTGGAACTGCAAACACAATGGGATCATCTTCTGGTTCACCCTCTACACCAACTATGCAGACACGACTGCCAACTTGGCAACAGAATGGTTTCGGCTCACTTGCATCAACACAGAATCAATTGGCTCATATGGACCATCTCAAGAGCAACGGATCTTTGGCGGACAATTTTGAGTTACTCTCATCTCTTGCTGATTCAGACCATAATGGACGAGTTGACCAAGGATTCTCATTTAATGAGATCAAGCATGGATTGGCTAGTTCAGATAAAGTTGACTGTTGTCACTTCTCCTCGGATGGAAAACTATTTGTTACTGGTGGTCGTGATAAAAAGGCTTCTCTATGGTGCACAAAATTGTTCAACCTGAAATCTACGCTTGAAGAGCACACTCAACGGATAACTGATGTTAGATTTAGCCCAAGCATGTTCTATGTTGCTACGTCCTCAGCCGATAAAACTGTAAAGGTTTGGGATGTTAATAATGCTGGTCATTCTCTTCGGACATTTACAGGGAATACAGCTGTGCTGTCACTAGACTTTCATCCAAGTAAACGTGGCCTTATTTGTTCTTGTGATAATAAGGAGATAAGATATTGGAATATTGCAAATGGTAGTTGTATTGGGATTTTCAAGGGTGGTGTAACTCAGGTGAGATTTCAACCTGGTTTGGGAAAGGTTCTTGCAGCAGCAGTAAATAATCAAATACTCATGTTTGACGCAGAAACTCTGAGTTGCAGATTTAAATTGCAGGGCCACATGAGCCTTGTTCGTTCAGTGTGCTGGCACTCTTCTGGAAATTATCTGGCATCTGTTTGTAATGATCTGGTTAAACTCTGGGCCATTGGATCTGATGGCAGCGGGGCATGTATTACGCAAGTGAATGCAGCTGAGAGCAAATTCAAGACCTGCGTTTTCCATCCTAGCTACAATATTTTGATCATTTGCTGCAGTGAGTCGCTTATGCTCTGGGACTTTGAGGAGAGCAAAAAACTTATTGTGCCTGCACACGACAAGCTTGTATCTGCATTGGCAGTATCAGATGTTACTGGATTGGTGGCCTCAGTTAGTCATGATAGGACTTTCAAGATCTGGAAGTGA
Protein sequence

>MsaT045396.1

MSQSKCDEPDKAYEIAELIAILLDAFIYDYLMKNQLYETARIFHDEGNVEQNIVMDAPGCSLFEWWSVFWELFMAKQGFSLSEPALSYLKAQEMRKQEEEIEKIQFILQSPAQLQQYGAETQEGVNANVLCPINNAPMVRQNRVTSNTMATKLNEDRLKTPLQGNAFDDRTFKIMNAYTRGQLPNEDCALLLNEIAPITGGQPSGQTLASALSILQEKLQQDQNQNQQLPGLQAMHSGMSAMMRSQAVVSEGSIYSVHGSNQGGSDLTLKGWPLAGLDEHHAELLHKHNLILPSQSSNQFSPRQQLMFQAQQDLVNPFVSGFDGKRPSLLVHNQNMVPWNDGQSNSVGGSIPNIVTPAQIGFPELPHLDLDMTHKASKSQVDRKQKRKVGSSSGLANSSGTANTMGSSSGSPSTPTMQTRLPTWQQNGFGSLASTQNQLAHMDHLKSNGSLADNFELLSSLADSDHNGRVDQGFSFNEIKHGLASSDKVDCCHFSSDGKLFVTGGRDKKASLWCTKLFNLKSTLEEHTQRITDVRFSPSMFYVATSSADKTVKVWDVNNAGHSLRTFTGNTAVLSLDFHPSKRGLICSCDNKEIRYWNIANGSCIGIFKGGVTQVRFQPGLGKVLAAAVNNQILMFDAETLSCRFKLQGHMSLVRSVCWHSSGNYLASVCNDLVKLWAIGSDGSGACITQVNAAESKFKTCVFHPSYNILIICCSESLMLWDFEESKKLIVPAHDKLVSALAVSDVTGLVASVSHDRTFKIWK*